Structured Review

Waters Corporation hilic separation
UPC 2 and UPLC separation of a Cer standard mixture. Positive ion mode chromatograms as shown for UPC 2 C18 (A.), UPC 2 <t>BEH</t> (B.), UPLC <t>HILIC</t> (C.), and UPLC reverse phase C18 (D.). The Cer standard is labeled above each chromatographic peak. The Cer standard mixture included Cer(d18:1/16:0), Cer(d18:1/18:0), Cer(d18:1/18:1), Cer(d18:1/20:0), Cer(d18:1/22:0), Cer(d18:1/24:0), and Cer(d18:1/24:1).
Hilic Separation, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 89/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hilic separation/product/Waters Corporation
Average 89 stars, based on 25 article reviews
Price from $9.99 to $1999.99
hilic separation - by Bioz Stars, 2020-07
89/100 stars

Images

1) Product Images from "UltraPerformance Convergence Chromatography-High Resolution Tandem Mass Spectrometry for Lipid Biomarker Profiling and Identification"

Article Title: UltraPerformance Convergence Chromatography-High Resolution Tandem Mass Spectrometry for Lipid Biomarker Profiling and Identification

Journal: Biomedical chromatography : BMC

doi: 10.1002/bmc.3822

UPC 2 and UPLC separation of a Cer standard mixture. Positive ion mode chromatograms as shown for UPC 2 C18 (A.), UPC 2 BEH (B.), UPLC HILIC (C.), and UPLC reverse phase C18 (D.). The Cer standard is labeled above each chromatographic peak. The Cer standard mixture included Cer(d18:1/16:0), Cer(d18:1/18:0), Cer(d18:1/18:1), Cer(d18:1/20:0), Cer(d18:1/22:0), Cer(d18:1/24:0), and Cer(d18:1/24:1).
Figure Legend Snippet: UPC 2 and UPLC separation of a Cer standard mixture. Positive ion mode chromatograms as shown for UPC 2 C18 (A.), UPC 2 BEH (B.), UPLC HILIC (C.), and UPLC reverse phase C18 (D.). The Cer standard is labeled above each chromatographic peak. The Cer standard mixture included Cer(d18:1/16:0), Cer(d18:1/18:0), Cer(d18:1/18:1), Cer(d18:1/20:0), Cer(d18:1/22:0), Cer(d18:1/24:0), and Cer(d18:1/24:1).

Techniques Used: Hydrophilic Interaction Liquid Chromatography, Labeling

2) Product Images from "The early diagnosis and monitoring of squamous cell carcinoma via saliva metabolomics"

Article Title: The early diagnosis and monitoring of squamous cell carcinoma via saliva metabolomics

Journal: Scientific Reports

doi: 10.1038/srep06802

The scores plot of the OPLS-DA model of the UPLC-MS date from control group, OSCC I–II group, and OSCC III–IV group. (A) RPLC in positive mode; (B) RPLC in negative mode; (C) HILIC in positive mode; (D) HILIC in negative mode.
Figure Legend Snippet: The scores plot of the OPLS-DA model of the UPLC-MS date from control group, OSCC I–II group, and OSCC III–IV group. (A) RPLC in positive mode; (B) RPLC in negative mode; (C) HILIC in positive mode; (D) HILIC in negative mode.

Techniques Used: Mass Spectrometry, Hydrophilic Interaction Liquid Chromatography

3) Product Images from "Single Spheroid Metabolomics: Optimizing Sample Preparation of Three-Dimensional Multicellular Tumor Spheroids"

Article Title: Single Spheroid Metabolomics: Optimizing Sample Preparation of Three-Dimensional Multicellular Tumor Spheroids

Journal: Metabolites

doi: 10.3390/metabo9120304

Heatmap displaying absolute amounts of metabolites normalized to total protein content (pmol/µg) of samples from single 3D MTS. Spheroids were treated for 24 h with either 200 µM KP1339, 20 µM oxaliplatin (OxPt), medium (CntrlOxPt) or medium with 0.5% DMSO (CntrlKP1339). Numbers in sample names refer to independent biological replicates. Extraction with cold methanol and internal standardization, measurement by HILIC high-resolution Orbitrap MS.
Figure Legend Snippet: Heatmap displaying absolute amounts of metabolites normalized to total protein content (pmol/µg) of samples from single 3D MTS. Spheroids were treated for 24 h with either 200 µM KP1339, 20 µM oxaliplatin (OxPt), medium (CntrlOxPt) or medium with 0.5% DMSO (CntrlKP1339). Numbers in sample names refer to independent biological replicates. Extraction with cold methanol and internal standardization, measurement by HILIC high-resolution Orbitrap MS.

Techniques Used: Hydrophilic Interaction Liquid Chromatography, Mass Spectrometry

Related Articles

Hydrophilic Interaction Liquid Chromatography:

Article Title: Single Spheroid Metabolomics: Optimizing Sample Preparation of Three-Dimensional Multicellular Tumor Spheroids
Article Snippet: .. We used high resolution Orbitrap MS coupled to Vanquish UPLC and with a HILIC separation, as described elsewhere [ ]. .. MS-data were acquired with positive–negative switching and the extracted ion chromatograms were evaluated with Thermo Trace Finder, with the help of external calibration and the internal standard, absolute amounts were calculated in pmol.

Article Title: Metabolic profiling of femoral muscle from rats at different periods of time after death
Article Snippet: .. For HILIC separation, the samples were analyzed using a 2.1-mm × 100-mm ACQUITY UPLC BEH 1.7-μm column (Waters, Ireland). ..

Article Title: The early diagnosis and monitoring of squamous cell carcinoma via saliva metabolomics
Article Snippet: .. HILIC separation was performed on ACQUITY UPLC™ BEH Amide column (100 mm × 2.1 mm i.d., 1.7 μm, Waters, Milford, USA). ..

Article Title: Mineral and Phytochemical Profiles and Antioxidant Activity of Herbal Material from Two Temperate Astragalus Species
Article Snippet: .. HILIC separation was performed on an Acquity UPLC BEH HILIC column (2.1 × 100 mm, 1.7 μ m, Waters). .. Data were acquired and processed using MassHunter software (Agilent).

Article Title: A rapid hydrophilic interaction liquid chromatographic determination of glimepiride in pharmaceutical formulations
Article Snippet: .. The analytical method comprised a fast ultrasound-assisted extraction with acetonitrile as a solvent followed by HILIC separation and quantification using a Waters Spherisorb S5 NH2 hydrophilic column with a mobile phase consisting of acetonitrile and aqueous acetate buffer (5.0 mM). .. The retention time of glimepiride increased slightly with decrease of mobile phase pH value from 6.8 to 5.8 and of acetonitrile content from 60% to 40%, indicating that both hydrophilic, ionic, and hydrophobic interactions were involved in the HILIC retention and elution mechanisms.

Article Title: In-column bonded phase polymerization for improved packing uniformity
Article Snippet: .. HILIC separation of ribonuclease B was performed using a Waters Acquity UPLC I-Class system (Milford, MA) with UV absorbance detection. ..

Article Title: UltraPerformance Convergence Chromatography-High Resolution Tandem Mass Spectrometry for Lipid Biomarker Profiling and Identification
Article Snippet: .. The HILIC separation was achieved using a BEH HILIC (1.7 μm; 2.1 × 100 mm) column (Waters, Milford, MA). ..

Article Title: Proteomics integrated with metabolomics: analysis of the internal causes of nutrient changes in alfalfa at different growth stages
Article Snippet: .. For HILIC separation, samples were analysed using a 2.1 × 100 mm ACQUITY UPLC BEH 1.7 μm column (Waters, Ireland). ..

Mass Spectrometry:

Article Title: Single Spheroid Metabolomics: Optimizing Sample Preparation of Three-Dimensional Multicellular Tumor Spheroids
Article Snippet: .. We used high resolution Orbitrap MS coupled to Vanquish UPLC and with a HILIC separation, as described elsewhere [ ]. .. MS-data were acquired with positive–negative switching and the extracted ion chromatograms were evaluated with Thermo Trace Finder, with the help of external calibration and the internal standard, absolute amounts were calculated in pmol.

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    Waters Corporation hilic separation
    UPC 2 and UPLC separation of a Cer standard mixture. Positive ion mode chromatograms as shown for UPC 2 C18 (A.), UPC 2 <t>BEH</t> (B.), UPLC <t>HILIC</t> (C.), and UPLC reverse phase C18 (D.). The Cer standard is labeled above each chromatographic peak. The Cer standard mixture included Cer(d18:1/16:0), Cer(d18:1/18:0), Cer(d18:1/18:1), Cer(d18:1/20:0), Cer(d18:1/22:0), Cer(d18:1/24:0), and Cer(d18:1/24:1).
    Hilic Separation, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 89/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic separation/product/Waters Corporation
    Average 89 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    hilic separation - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    Image Search Results


    UPC 2 and UPLC separation of a Cer standard mixture. Positive ion mode chromatograms as shown for UPC 2 C18 (A.), UPC 2 BEH (B.), UPLC HILIC (C.), and UPLC reverse phase C18 (D.). The Cer standard is labeled above each chromatographic peak. The Cer standard mixture included Cer(d18:1/16:0), Cer(d18:1/18:0), Cer(d18:1/18:1), Cer(d18:1/20:0), Cer(d18:1/22:0), Cer(d18:1/24:0), and Cer(d18:1/24:1).

    Journal: Biomedical chromatography : BMC

    Article Title: UltraPerformance Convergence Chromatography-High Resolution Tandem Mass Spectrometry for Lipid Biomarker Profiling and Identification

    doi: 10.1002/bmc.3822

    Figure Lengend Snippet: UPC 2 and UPLC separation of a Cer standard mixture. Positive ion mode chromatograms as shown for UPC 2 C18 (A.), UPC 2 BEH (B.), UPLC HILIC (C.), and UPLC reverse phase C18 (D.). The Cer standard is labeled above each chromatographic peak. The Cer standard mixture included Cer(d18:1/16:0), Cer(d18:1/18:0), Cer(d18:1/18:1), Cer(d18:1/20:0), Cer(d18:1/22:0), Cer(d18:1/24:0), and Cer(d18:1/24:1).

    Article Snippet: The HILIC separation was achieved using a BEH HILIC (1.7 μm; 2.1 × 100 mm) column (Waters, Milford, MA).

    Techniques: Hydrophilic Interaction Liquid Chromatography, Labeling

    The scores plot of the OPLS-DA model of the UPLC-MS date from control group, OSCC I–II group, and OSCC III–IV group. (A) RPLC in positive mode; (B) RPLC in negative mode; (C) HILIC in positive mode; (D) HILIC in negative mode.

    Journal: Scientific Reports

    Article Title: The early diagnosis and monitoring of squamous cell carcinoma via saliva metabolomics

    doi: 10.1038/srep06802

    Figure Lengend Snippet: The scores plot of the OPLS-DA model of the UPLC-MS date from control group, OSCC I–II group, and OSCC III–IV group. (A) RPLC in positive mode; (B) RPLC in negative mode; (C) HILIC in positive mode; (D) HILIC in negative mode.

    Article Snippet: HILIC separation was performed on ACQUITY UPLC™ BEH Amide column (100 mm × 2.1 mm i.d., 1.7 μm, Waters, Milford, USA).

    Techniques: Mass Spectrometry, Hydrophilic Interaction Liquid Chromatography

    Heatmap displaying absolute amounts of metabolites normalized to total protein content (pmol/µg) of samples from single 3D MTS. Spheroids were treated for 24 h with either 200 µM KP1339, 20 µM oxaliplatin (OxPt), medium (CntrlOxPt) or medium with 0.5% DMSO (CntrlKP1339). Numbers in sample names refer to independent biological replicates. Extraction with cold methanol and internal standardization, measurement by HILIC high-resolution Orbitrap MS.

    Journal: Metabolites

    Article Title: Single Spheroid Metabolomics: Optimizing Sample Preparation of Three-Dimensional Multicellular Tumor Spheroids

    doi: 10.3390/metabo9120304

    Figure Lengend Snippet: Heatmap displaying absolute amounts of metabolites normalized to total protein content (pmol/µg) of samples from single 3D MTS. Spheroids were treated for 24 h with either 200 µM KP1339, 20 µM oxaliplatin (OxPt), medium (CntrlOxPt) or medium with 0.5% DMSO (CntrlKP1339). Numbers in sample names refer to independent biological replicates. Extraction with cold methanol and internal standardization, measurement by HILIC high-resolution Orbitrap MS.

    Article Snippet: We used high resolution Orbitrap MS coupled to Vanquish UPLC and with a HILIC separation, as described elsewhere [ ].

    Techniques: Hydrophilic Interaction Liquid Chromatography, Mass Spectrometry