hilic column  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hilic column
    Hilic Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic column/product/Thermo Fisher
    Average 93 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    hilic column - by Bioz Stars, 2020-07
    93/100 stars

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    Related Articles

    Hydrophilic Interaction Liquid Chromatography:

    Article Title: Plasma Ceramides as Prognostic Biomarkers and Their Arterial and Myocardial Tissue Correlates in Acute Myocardial Infarction
    Article Snippet: .. Extracted lipids were separated on an HILIC column (Thermo Fisher Scientific, Waltham, Massachusetts) (100 × 2.1 mm; particle size 2.6 μm) using an Agilent 1260 LC system coupled to an Agilent 6430 triple quadrupole mass spectrometer (Agilent Technologies, Santa Clara, California). .. Briefly, the HILIC column was equilibrated with 100% mobile phase A (acetonitrile/water [95:5] with 10 mmol/l ammonium acetate, pH 8.0), increasing to 20% mobile phase B (acetonitrile/water [50:50] with 10 mmol/l ammonium acetate, pH 8.0) in 5 min, then held for 5 min.

    Article Title: Metabolites of organophosphate esters in urine from the United States: Concentrations, temporal variability, and exposure assessment
    Article Snippet: .. Eleven OPE metabolites and 9 internal standards were separated by a HILIC column (100 mm × 2.1 mm, 5 μm; Thermo, Waltham, MA, U.S.) connected to a Betasil C18 guard column (20 mm × 2.1 mm, 5 μm; Thermo). .. The mobile phase consisted of HPLC-grade water (10 mM ammonium acetate): methanol (2:3 v/v) (A) and acetonitrile (B), used at a flow rate of 200 μL/min.

    Article Title: Plasma Ceramides as Prognostic Biomarkers and Their Arterial and Myocardial Tissue Correlates in Acute Myocardial Infarction
    Article Snippet: .. Briefly, the HILIC column was equilibrated with 100% mobile phase A (acetonitrile/water [95:5] with 10 mmol/l ammonium acetate, pH 8.0), increasing to 20% mobile phase B (acetonitrile/water [50:50] with 10 mmol/l ammonium acetate, pH 8.0) in 5 min, then held for 5 min. ..

    Article Title: Analysis of Stable Isotope Assisted Metabolomics Data Acquired by High Resolution Mass Spectrometry
    Article Snippet: .. The HILIC column was a Thermo Accucore HILIC column (100×3 mm i.d., 2.6 μm, part number: 17526-103030). .. The RP column was a Waters ACQUITY UPLC HSS T3 column (150×2.1 mm i.d., 1.8 μm, part number: 186003540).

    Article Title: The Power of LC-MS Based Multiomics: Exploring Adipogenic Differentiation of Human Mesenchymal Stem/Stromal Cells
    Article Snippet: .. The RP separation was carried out during the equilibration of the HILIC column with a total run time of 32 min. High-resolution mass spectrometry was conducted on a high field Thermo Scientific™ Q Exactive HF™ quadrupole-Orbitrap mass spectrometer equipped with an electrospray source. ..

    Article Title: Analytes related to erythrocyte metabolism are reliable biomarkers for preanalytical error due to delayed plasma processing in metabolomics studies
    Article Snippet: .. For aliquots 2–4, chromatographic separation was achieved using an ACQUITY UPLC (Waters) equipped with a Waters BEH C18 (aliquot 2 and 3) or HILIC column (aliquot 4) followed by analysis with a QExactive high resolution mass spectrometer (Thermo-Finnigan). ..

    Article Title: Analysis of Stable Isotope Assisted Metabolomics Data Acquired by High Resolution Mass Spectrometry
    Article Snippet: .. The HILIC column was operated as follows: mobile phase A was 10 mM ammonium acetate (pH adjusted to 3.25 with acetate) and mobile phase B was acetonitrile. ..

    Mass Spectrometry:

    Article Title: Plasma Ceramides as Prognostic Biomarkers and Their Arterial and Myocardial Tissue Correlates in Acute Myocardial Infarction
    Article Snippet: .. Extracted lipids were separated on an HILIC column (Thermo Fisher Scientific, Waltham, Massachusetts) (100 × 2.1 mm; particle size 2.6 μm) using an Agilent 1260 LC system coupled to an Agilent 6430 triple quadrupole mass spectrometer (Agilent Technologies, Santa Clara, California). .. Briefly, the HILIC column was equilibrated with 100% mobile phase A (acetonitrile/water [95:5] with 10 mmol/l ammonium acetate, pH 8.0), increasing to 20% mobile phase B (acetonitrile/water [50:50] with 10 mmol/l ammonium acetate, pH 8.0) in 5 min, then held for 5 min.

    Article Title: The Power of LC-MS Based Multiomics: Exploring Adipogenic Differentiation of Human Mesenchymal Stem/Stromal Cells
    Article Snippet: .. The RP separation was carried out during the equilibration of the HILIC column with a total run time of 32 min. High-resolution mass spectrometry was conducted on a high field Thermo Scientific™ Q Exactive HF™ quadrupole-Orbitrap mass spectrometer equipped with an electrospray source. ..

    Article Title: Analytes related to erythrocyte metabolism are reliable biomarkers for preanalytical error due to delayed plasma processing in metabolomics studies
    Article Snippet: .. For aliquots 2–4, chromatographic separation was achieved using an ACQUITY UPLC (Waters) equipped with a Waters BEH C18 (aliquot 2 and 3) or HILIC column (aliquot 4) followed by analysis with a QExactive high resolution mass spectrometer (Thermo-Finnigan). ..

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    Thermo Fisher accucore 150 amide hilic lc column
    Chromatogram of dextran ladder (a), N -glycan profile of NIST mAb prepared with GlycanAssure HyPerformance APTS kit and analyzed on <t>Accucore</t> 150 Amide <t>HILIC</t> LC Column and Vanquish Horizon UHPLC equipped with FLD zoomed out (b) and zoomed in views (c).
    Accucore 150 Amide Hilic Lc Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accucore 150 amide hilic lc column/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    accucore 150 amide hilic lc column - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    93
    Thermo Fisher acclaim hilic 10
    HPLC-UV chromatograms acquired during the time showing the disappearance of GANT61 and the formation of GANT61-D. Sample: GANT61 in CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v) (1 mg/ml), column: Acclaim <t>HILIC-10,</t> 3 μm (150 × 4.6 mm I.D.), mobile phase: CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v), flow-rate: 1.0 ml/min, detection: UV at 254 nm. (A) t = 0 min; (B) t = 30 min; (C) t = 24 h. The ESI-MS (pos.) spectrum of GANT61-D is included in the box for unequivocal identification.
    Acclaim Hilic 10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acclaim hilic 10/product/Thermo Fisher
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    acclaim hilic 10 - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Chromatogram of dextran ladder (a), N -glycan profile of NIST mAb prepared with GlycanAssure HyPerformance APTS kit and analyzed on Accucore 150 Amide HILIC LC Column and Vanquish Horizon UHPLC equipped with FLD zoomed out (b) and zoomed in views (c).

    Journal: BioMed Research International

    Article Title: Development of Two Analytical Methods Based on Reverse Phase Chromatographic and SDS-PAGE Gel for Assessment of Deglycosylation Yield in N-Glycan Mapping

    doi: 10.1155/2018/3909674

    Figure Lengend Snippet: Chromatogram of dextran ladder (a), N -glycan profile of NIST mAb prepared with GlycanAssure HyPerformance APTS kit and analyzed on Accucore 150 Amide HILIC LC Column and Vanquish Horizon UHPLC equipped with FLD zoomed out (b) and zoomed in views (c).

    Article Snippet: UHPLC-HILIC for Analysis of N -Glycan Mapping Labeled N -glycans were separated with Thermo Scientific Accucore 150 Amide HILIC LC Column (2.6 μ m, 150 A, 2.1 × 150 mm) at 50°C with Thermo Scientific Vanquish Horizon UHPLC System equipped with 2 μ l flow cell FLD at 455 nm and 500 nm excitation and emission wavelength, respectively, at high power lamp mode and 10 Hz of data collection rate.

    Techniques: Hydrophilic Interaction Liquid Chromatography

    HPLC-UV chromatograms acquired during the time showing the disappearance of GANT61 and the formation of GANT61-D. Sample: GANT61 in CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v) (1 mg/ml), column: Acclaim HILIC-10, 3 μm (150 × 4.6 mm I.D.), mobile phase: CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v), flow-rate: 1.0 ml/min, detection: UV at 254 nm. (A) t = 0 min; (B) t = 30 min; (C) t = 24 h. The ESI-MS (pos.) spectrum of GANT61-D is included in the box for unequivocal identification.

    Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

    Article Title: Chemical, computational and functional insights into the chemical stability of the Hedgehog pathway inhibitor GANT61

    doi: 10.1080/14756366.2017.1419221

    Figure Lengend Snippet: HPLC-UV chromatograms acquired during the time showing the disappearance of GANT61 and the formation of GANT61-D. Sample: GANT61 in CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v) (1 mg/ml), column: Acclaim HILIC-10, 3 μm (150 × 4.6 mm I.D.), mobile phase: CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v), flow-rate: 1.0 ml/min, detection: UV at 254 nm. (A) t = 0 min; (B) t = 30 min; (C) t = 24 h. The ESI-MS (pos.) spectrum of GANT61-D is included in the box for unequivocal identification.

    Article Snippet: The column used was the Acclaim HILIC-10, 3 µm (150 × 4.6 mm I.D.), purchased from Thermo Scientific (Waltham, MA).

    Techniques: High Performance Liquid Chromatography, Hydrophilic Interaction Liquid Chromatography, Flow Cytometry, Mass Spectrometry

    HPLC-UV chromatograms acquired during the time showing the disappearance of GANT61 and the formation of GANT61-D. Sample: GANT61 in CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v) (1 mg/ml), column: Acclaim HILIC-10, 3 μm (150 × 4.6 mm I.D.), mobile phase: CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v), flow-rate: 1.0 ml/min, detection: UV at 254 nm. (A) t = 0 min; (B) t = 30 min; (C) t = 24 h. The ESI-MS (pos.) spectrum of GANT61-D is included in the box for unequivocal identification.

    Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

    Article Title: Chemical, computational and functional insights into the chemical stability of the Hedgehog pathway inhibitor GANT61

    doi: 10.1080/14756366.2017.1419221

    Figure Lengend Snippet: HPLC-UV chromatograms acquired during the time showing the disappearance of GANT61 and the formation of GANT61-D. Sample: GANT61 in CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v) (1 mg/ml), column: Acclaim HILIC-10, 3 μm (150 × 4.6 mm I.D.), mobile phase: CH 3 CN/100 mM NH 4 OAc (pH 4.5) = 95/5 (v/v), flow-rate: 1.0 ml/min, detection: UV at 254 nm. (A) t = 0 min; (B) t = 30 min; (C) t = 24 h. The ESI-MS (pos.) spectrum of GANT61-D is included in the box for unequivocal identification.

    Article Snippet: The column used was the Acclaim HILIC-10, 3 µm (150 × 4.6 mm I.D.), purchased from Thermo Scientific (Waltham, MA).

    Techniques: High Performance Liquid Chromatography, Hydrophilic Interaction Liquid Chromatography, Flow Cytometry, Mass Spectrometry