Structured Review

Agilent technologies high sensitivity dna
High Sensitivity Dna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 98/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/high sensitivity dna/product/Agilent technologies
Average 98 stars, based on 4 article reviews
Price from $9.99 to $1999.99
high sensitivity dna - by Bioz Stars, 2020-04
98/100 stars

Images

Related Articles

Clone Assay:

Article Title: Maternal and zygotic gene regulatory effects of endogenous RNAi pathways
Article Snippet: Both directly cloned and RppH-treated libraries were prepared with a starting amount of 200ng and amplified in 16 PCR cycles. .. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).

Amplification:

Article Title: Maternal and zygotic gene regulatory effects of endogenous RNAi pathways
Article Snippet: Amplified libraries were purified by running an 8% TBE gel and size-selected for 18–40 nts. .. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).

Article Title: GADD45 promotes locus-specific DNA demethylation and 2C cycling in embryonic stem cells
Article Snippet: Libraries were prepared with a starting amount of 2.36 ng of fragmented cDNA and were amplified in 11 PCR cycles. .. Libraries were profiled in a high-sensitivity DNA on a 2100 Bioanalyzer (Agilent Technologies) and quantified using the Qubit dsDNA HS assay kit in a Qubit 2.0 fluorometer (Life Technologies).

Article Title: Cooperation of Dnmt3a R878H with Nras G12D promotes leukemogenesis in knock-in mice: a pilot study
Article Snippet: Finally, PCR amplification was carried out and the PCR products were purified by VAHTSTM DNA Clean Beads. .. The obtained library was then checked using Agilent High Sensitivity DNA Reagent with a 2100 Bioanalyzer, and 200-bp paired-end sequencing was carried out on an Illumina HiSeq.

Synthesized:

Article Title: Cooperation of Dnmt3a R878H with Nras G12D promotes leukemogenesis in knock-in mice: a pilot study
Article Snippet: Using mRNA as template, a single chain cDNA was synthesized by six base random primers, followed by two strand cDNA synthesis reaction, then VAHTSTM DNA Clean Beads was used to purify double chain cDNA. .. The obtained library was then checked using Agilent High Sensitivity DNA Reagent with a 2100 Bioanalyzer, and 200-bp paired-end sequencing was carried out on an Illumina HiSeq.

Derivative Assay:

Article Title: Role for the transcriptional activator ZRF1 in early metastatic events in breast cancer progression and endocrine resistance
Article Snippet: RNA sequencing For each replicate, RNA was isolated from 7 days old spheroids derived from control and shZRF1 MCF7 cells using Direct-zol RNA MiniPrep kit (Zymo Research). .. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).

Ligation:

Article Title: Microevolution of Anthrax from a Young Ancestor (M.A.Y.A.) Suggests a Soil-Borne Life Cycle of Bacillus anthracis
Article Snippet: .. The 2100 Bioanalyzer (Agilent Technologies) was used for quality control after end-repair, size selection and adapter ligation following the instructions of Agilent High Sensitivity DNA Quick Start Guide (Agilent Technologies). .. DNA concentrations of the libraries were measured using the Qubit dsDNA HS Assay Kit (Life Technologies) and the DNA was diluted to 44.2 pM.

Cell Culture:

Article Title: GADD45 promotes locus-specific DNA demethylation and 2C cycling in embryonic stem cells
Article Snippet: Libraries were profiled in a high-sensitivity DNA on a 2100 Bioanalyzer (Agilent Technologies) and quantified using the Qubit dsDNA HS assay kit in a Qubit 2.0 fluorometer (Life Technologies). .. For the in vitro development assay, mouse preimplantation embryos were collected as described above and then cultured in EmbryoMax human tubal fluid medium (Millipore) for 48 h at 37°C and 5% CO2 .

Generated:

Article Title: DNA analysis of low- and high-density fractions defines heterogeneous subpopulations of small extracellular vesicles based on their DNA cargo and topology
Article Snippet: DNA isolation and quantification Small EV samples dissolved in 200 µL of PBS solution generated by iodixanol density gradient fractionation, underwent DNA extraction and analysis. .. After DNA isolation, samples were eluted in 200 µL of DEPC water, and DNA size and concentrations were analysed using the Bioanalyzer 2100 instrument with High Sensitivity DNA and DNA 7500 kits (Agilent Technologies Inc., Palo Alto, CA, USA) according to the manufacturer´s protocols.

Polymerase Chain Reaction:

Article Title: Maternal and zygotic gene regulatory effects of endogenous RNAi pathways
Article Snippet: Both directly cloned and RppH-treated libraries were prepared with a starting amount of 200ng and amplified in 16 PCR cycles. .. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).

Article Title: GADD45 promotes locus-specific DNA demethylation and 2C cycling in embryonic stem cells
Article Snippet: Libraries were prepared with a starting amount of 2.36 ng of fragmented cDNA and were amplified in 11 PCR cycles. .. Libraries were profiled in a high-sensitivity DNA on a 2100 Bioanalyzer (Agilent Technologies) and quantified using the Qubit dsDNA HS assay kit in a Qubit 2.0 fluorometer (Life Technologies).

Article Title: Cooperation of Dnmt3a R878H with Nras G12D promotes leukemogenesis in knock-in mice: a pilot study
Article Snippet: Finally, PCR amplification was carried out and the PCR products were purified by VAHTSTM DNA Clean Beads. .. The obtained library was then checked using Agilent High Sensitivity DNA Reagent with a 2100 Bioanalyzer, and 200-bp paired-end sequencing was carried out on an Illumina HiSeq.

Article Title: Microevolution of Anthrax from a Young Ancestor (M.A.Y.A.) Suggests a Soil-Borne Life Cycle of Bacillus anthracis
Article Snippet: The 2100 Bioanalyzer (Agilent Technologies) was used for quality control after end-repair, size selection and adapter ligation following the instructions of Agilent High Sensitivity DNA Quick Start Guide (Agilent Technologies). .. Emulsion PCR and enrichment of template positive Ion Sphere Particles were performed according to the Ion PGM Template OT2 400 Kit (Life Technologies).

Article Title: RNA-Seq and iTRAQ Reveal the Dwarfing Mechanism of Dwarf Polish Wheat (Triticum polonicum L.)
Article Snippet: .. The PCR products were purified using the AMPure XP bead system and quantified using the Agilent high sensitivity DNA (Agilent Technologies, CA, USA) on the Agilent 2100 bioanalyzer system. .. Finally, the four-coded samples were clustered by the cBot cluster generation system using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina) according to the user manual, and then sequenced using the 100 bp protocol on an Illumina Hiseq 2000 platform.

Sonication:

Article Title: Microevolution of Anthrax from a Young Ancestor (M.A.Y.A.) Suggests a Soil-Borne Life Cycle of Bacillus anthracis
Article Snippet: For this, library preparation was performed according to the Ion Xpress Plus Fragment Library Preparation Guide (Life Technologies) using a sonication method involving the Bioruptor system UCD-200 (Life Technologies) and subsequent end-repair (Life Technologies). .. The 2100 Bioanalyzer (Agilent Technologies) was used for quality control after end-repair, size selection and adapter ligation following the instructions of Agilent High Sensitivity DNA Quick Start Guide (Agilent Technologies).

DNA Extraction:

Article Title: DNA analysis of low- and high-density fractions defines heterogeneous subpopulations of small extracellular vesicles based on their DNA cargo and topology
Article Snippet: .. After DNA isolation, samples were eluted in 200 µL of DEPC water, and DNA size and concentrations were analysed using the Bioanalyzer 2100 instrument with High Sensitivity DNA and DNA 7500 kits (Agilent Technologies Inc., Palo Alto, CA, USA) according to the manufacturer´s protocols. .. The dsDNA High Sensitivity and ssDNA Assay Kits (Thermo Fisher Scientific) were also used to quantify ss/ds EV-associated DNA using the Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, California, CA, USA).

RNA Sequencing Assay:

Article Title: Maternal and zygotic gene regulatory effects of endogenous RNAi pathways
Article Snippet: Paragraph title: RppH treatment and library preparation for small RNA sequencing ... Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).

Article Title: Quantifying post-transcriptional regulation in the development of Drosophila melanogaster
Article Snippet: Paragraph title: RNA-Sequencing ... Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).

Article Title: Role for the transcriptional activator ZRF1 in early metastatic events in breast cancer progression and endocrine resistance
Article Snippet: Paragraph title: RNA sequencing ... Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).

Article Title: Cooperation of Dnmt3a R878H with Nras G12D promotes leukemogenesis in knock-in mice: a pilot study
Article Snippet: Paragraph title: RNA-seq ... The obtained library was then checked using Agilent High Sensitivity DNA Reagent with a 2100 Bioanalyzer, and 200-bp paired-end sequencing was carried out on an Illumina HiSeq.

Magnetic Beads:

Article Title: RNA-Seq and iTRAQ Reveal the Dwarfing Mechanism of Dwarf Polish Wheat (Triticum polonicum L.)
Article Snippet: Library construction and sequencing mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Life Technologies, CA, USA) and transcribed to cDNA using random oligonucleotides with M-MuLV Rease Transcriptase (RNase H- ) (TaKaRa) and DNA polymerase I and RNase H (TaKaRa). .. The PCR products were purified using the AMPure XP bead system and quantified using the Agilent high sensitivity DNA (Agilent Technologies, CA, USA) on the Agilent 2100 bioanalyzer system.

Isolation:

Article Title: GADD45 promotes locus-specific DNA demethylation and 2C cycling in embryonic stem cells
Article Snippet: Paragraph title: Isolation and culture of preimplantation embryos ... Libraries were profiled in a high-sensitivity DNA on a 2100 Bioanalyzer (Agilent Technologies) and quantified using the Qubit dsDNA HS assay kit in a Qubit 2.0 fluorometer (Life Technologies).

Article Title: Role for the transcriptional activator ZRF1 in early metastatic events in breast cancer progression and endocrine resistance
Article Snippet: RNA sequencing For each replicate, RNA was isolated from 7 days old spheroids derived from control and shZRF1 MCF7 cells using Direct-zol RNA MiniPrep kit (Zymo Research). .. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).

Article Title: DNA analysis of low- and high-density fractions defines heterogeneous subpopulations of small extracellular vesicles based on their DNA cargo and topology
Article Snippet: DNA was isolated from all DNase-treated and non-treated sEV fractions using the QIAamp DNA Mini kit (Qiagen) following the manufacturer’s instructions. .. After DNA isolation, samples were eluted in 200 µL of DEPC water, and DNA size and concentrations were analysed using the Bioanalyzer 2100 instrument with High Sensitivity DNA and DNA 7500 kits (Agilent Technologies Inc., Palo Alto, CA, USA) according to the manufacturer´s protocols.

Purification:

Article Title: Maternal and zygotic gene regulatory effects of endogenous RNAi pathways
Article Snippet: Amplified libraries were purified by running an 8% TBE gel and size-selected for 18–40 nts. .. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).

Article Title: Cooperation of Dnmt3a R878H with Nras G12D promotes leukemogenesis in knock-in mice: a pilot study
Article Snippet: Finally, PCR amplification was carried out and the PCR products were purified by VAHTSTM DNA Clean Beads. .. The obtained library was then checked using Agilent High Sensitivity DNA Reagent with a 2100 Bioanalyzer, and 200-bp paired-end sequencing was carried out on an Illumina HiSeq.

Article Title: RNA-Seq and iTRAQ Reveal the Dwarfing Mechanism of Dwarf Polish Wheat (Triticum polonicum L.)
Article Snippet: .. The PCR products were purified using the AMPure XP bead system and quantified using the Agilent high sensitivity DNA (Agilent Technologies, CA, USA) on the Agilent 2100 bioanalyzer system. .. Finally, the four-coded samples were clustered by the cBot cluster generation system using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina) according to the user manual, and then sequenced using the 100 bp protocol on an Illumina Hiseq 2000 platform.

Sequencing:

Article Title: GADD45 promotes locus-specific DNA demethylation and 2C cycling in embryonic stem cells
Article Snippet: Embryos were collected in M2 medium (Sigma) supplemented with 0.3 mg/mL hyaluronidase (Sigma), washed twice with PBS, and directly transferred into lysis buffer (Smart Seq version 4 ultralow input RNA kit for sequencing, Takara). .. Libraries were profiled in a high-sensitivity DNA on a 2100 Bioanalyzer (Agilent Technologies) and quantified using the Qubit dsDNA HS assay kit in a Qubit 2.0 fluorometer (Life Technologies).

Article Title: Cooperation of Dnmt3a R878H with Nras G12D promotes leukemogenesis in knock-in mice: a pilot study
Article Snippet: .. The obtained library was then checked using Agilent High Sensitivity DNA Reagent with a 2100 Bioanalyzer, and 200-bp paired-end sequencing was carried out on an Illumina HiSeq. .. Quantitative real-time RT-PCR The cDNA was synthesized used M-MLV reverse transcriptase (Invitrogen).

Article Title: Microevolution of Anthrax from a Young Ancestor (M.A.Y.A.) Suggests a Soil-Borne Life Cycle of Bacillus anthracis
Article Snippet: Paragraph title: Ion Torrent next generation whole genome sequencing ... The 2100 Bioanalyzer (Agilent Technologies) was used for quality control after end-repair, size selection and adapter ligation following the instructions of Agilent High Sensitivity DNA Quick Start Guide (Agilent Technologies).

Article Title: Maternal and zygotic gene regulatory effects of endogenous RNAi pathways
Article Snippet: Paragraph title: Library preparation for mRNA sequencing ... Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).

Article Title: RNA-Seq and iTRAQ Reveal the Dwarfing Mechanism of Dwarf Polish Wheat (Triticum polonicum L.)
Article Snippet: Paragraph title: Library construction and sequencing ... The PCR products were purified using the AMPure XP bead system and quantified using the Agilent high sensitivity DNA (Agilent Technologies, CA, USA) on the Agilent 2100 bioanalyzer system.

Chromatin Immunoprecipitation:

Article Title: Microevolution of Anthrax from a Young Ancestor (M.A.Y.A.) Suggests a Soil-Borne Life Cycle of Bacillus anthracis
Article Snippet: The 2100 Bioanalyzer (Agilent Technologies) was used for quality control after end-repair, size selection and adapter ligation following the instructions of Agilent High Sensitivity DNA Quick Start Guide (Agilent Technologies). .. Chip loading was carried out according to the manual using Ion 318 Chip Kit v2 (Life Technologies) in combination with Ion PGM Sequencing 400 Kit-chemistry (Life Technologies).

Selection:

Article Title: Microevolution of Anthrax from a Young Ancestor (M.A.Y.A.) Suggests a Soil-Borne Life Cycle of Bacillus anthracis
Article Snippet: .. The 2100 Bioanalyzer (Agilent Technologies) was used for quality control after end-repair, size selection and adapter ligation following the instructions of Agilent High Sensitivity DNA Quick Start Guide (Agilent Technologies). .. DNA concentrations of the libraries were measured using the Qubit dsDNA HS Assay Kit (Life Technologies) and the DNA was diluted to 44.2 pM.

Sample Prep:

Article Title: Quantifying post-transcriptional regulation in the development of Drosophila melanogaster
Article Snippet: RNA-Sequencing NGS library prep was performed with Illumina’s TruSeq stranded mRNA LT Sample Prep Kit following Illumina’s standard protocol (Part #15031047 Rev. .. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).

Article Title: GADD45 promotes locus-specific DNA demethylation and 2C cycling in embryonic stem cells
Article Snippet: Sample preparation was done according to the manufacturer's recommendations. cDNA was amplified using 11 PCR cycles. .. Libraries were profiled in a high-sensitivity DNA on a 2100 Bioanalyzer (Agilent Technologies) and quantified using the Qubit dsDNA HS assay kit in a Qubit 2.0 fluorometer (Life Technologies).

Article Title: Role for the transcriptional activator ZRF1 in early metastatic events in breast cancer progression and endocrine resistance
Article Snippet: NGS library preparation was performed with Illumina’s TruSeq stranded RNA LT Sample Prep Kit following Illumina’s standard protocol (Part # 15031048 Rev. .. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).

Article Title: Maternal and zygotic gene regulatory effects of endogenous RNAi pathways
Article Snippet: Library preparation for mRNA sequencing NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illumina’s standard protocol (Part # 15031047 Rev. .. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).

In Vitro:

Article Title: GADD45 promotes locus-specific DNA demethylation and 2C cycling in embryonic stem cells
Article Snippet: Libraries were profiled in a high-sensitivity DNA on a 2100 Bioanalyzer (Agilent Technologies) and quantified using the Qubit dsDNA HS assay kit in a Qubit 2.0 fluorometer (Life Technologies). .. For the in vitro development assay, mouse preimplantation embryos were collected as described above and then cultured in EmbryoMax human tubal fluid medium (Millipore) for 48 h at 37°C and 5% CO2 .

Next-Generation Sequencing:

Article Title: Maternal and zygotic gene regulatory effects of endogenous RNAi pathways
Article Snippet: NGS library prep was performed with NEXTflex Small RNA-Seq Kit V3 following Step A to Step G of Bioo Scientific`s standard protocol (V16.06). .. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).

Article Title: Quantifying post-transcriptional regulation in the development of Drosophila melanogaster
Article Snippet: RNA-Sequencing NGS library prep was performed with Illumina’s TruSeq stranded mRNA LT Sample Prep Kit following Illumina’s standard protocol (Part #15031047 Rev. .. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).

Article Title: GADD45 promotes locus-specific DNA demethylation and 2C cycling in embryonic stem cells
Article Snippet: NGS library preparation was performed using NuGEN's Ovation ultralow system V2 1-96 (2014). .. Libraries were profiled in a high-sensitivity DNA on a 2100 Bioanalyzer (Agilent Technologies) and quantified using the Qubit dsDNA HS assay kit in a Qubit 2.0 fluorometer (Life Technologies).

Article Title: Role for the transcriptional activator ZRF1 in early metastatic events in breast cancer progression and endocrine resistance
Article Snippet: NGS library preparation was performed with Illumina’s TruSeq stranded RNA LT Sample Prep Kit following Illumina’s standard protocol (Part # 15031048 Rev. .. Libraries were profiled in a High Sensitivity DNA on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies).

Fractionation:

Article Title: DNA analysis of low- and high-density fractions defines heterogeneous subpopulations of small extracellular vesicles based on their DNA cargo and topology
Article Snippet: DNA isolation and quantification Small EV samples dissolved in 200 µL of PBS solution generated by iodixanol density gradient fractionation, underwent DNA extraction and analysis. .. After DNA isolation, samples were eluted in 200 µL of DEPC water, and DNA size and concentrations were analysed using the Bioanalyzer 2100 instrument with High Sensitivity DNA and DNA 7500 kits (Agilent Technologies Inc., Palo Alto, CA, USA) according to the manufacturer´s protocols.

Lysis:

Article Title: GADD45 promotes locus-specific DNA demethylation and 2C cycling in embryonic stem cells
Article Snippet: Embryos were collected in M2 medium (Sigma) supplemented with 0.3 mg/mL hyaluronidase (Sigma), washed twice with PBS, and directly transferred into lysis buffer (Smart Seq version 4 ultralow input RNA kit for sequencing, Takara). .. Libraries were profiled in a high-sensitivity DNA on a 2100 Bioanalyzer (Agilent Technologies) and quantified using the Qubit dsDNA HS assay kit in a Qubit 2.0 fluorometer (Life Technologies).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Agilent technologies high sensitivity dna chip
    ORF37’s DNase activity is required for efficient packaging of viral <t>DNA</t> and nuclear egress. (A) <t>qPCR</t> analysis of BamHI-digested total and encapsidated (DNase I-resistant) DNAs from 72-hpi 293L/wt and 293L/Q129H cells. When normalized to the total viral DNA, DNA encapsidation in 293L/Q129H cells was significantly reduced compared to the wt control; ***, P
    High Sensitivity Dna Chip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 522 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high sensitivity dna chip/product/Agilent technologies
    Average 99 stars, based on 522 article reviews
    Price from $9.99 to $1999.99
    high sensitivity dna chip - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Agilent technologies high sensitivity dna kit
    Amplification and cloning of sg mRNA leader–body junctions generated from additional functional TRSs located in the genomic region between identified TRS5 and TRS6. ( A ) Diagram indicating the positions of the primers used and the estimated size for the amplified leader–body junction sequences (thick black line). The white open box represents ORF5, and the short black box represents the leader sequence in transcribed sg mRNAs. ( B ) MA104 cells were either mock-infected (M) or infected with SHFVic at an MOI of 1. At 24 hpi, total intracellular <t>RNA</t> was extracted and subjected to RT-PCR, and the products were separated on a 2% <t>DNA</t> gel. The band with the size estimated for the leader–body junction in sg mRNA5 produced from the known TRS5 is indicated by an arrow. PCR bands with sizes estimated for the leader–body junctions of ∼1.7-kb sg mRNAs are indicated by a bracket. L, ladder. ( C ) The bracketed region of the gel was excised, and the DNA was extracted and cloned into a TA vector. Forty colonies were randomly selected and subjected to restriction digestion, and the inserts were separated by gel electrophoresis. The results from 10 representative clones are shown. L, ladder. ( D ) Diagram showing the locations of the known and previously unreported body TRSs. The TRSs are indicated by black vertical bars. The previously unreported functional TRSs are within a dotted line box. The ORFs encoded by the individual sg mRNAs are indicated by white open boxes. 5-C, ORF5-C-68aa; L, leader region.
    High Sensitivity Dna Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 371 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high sensitivity dna kit/product/Agilent technologies
    Average 99 stars, based on 371 article reviews
    Price from $9.99 to $1999.99
    high sensitivity dna kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    96
    Agilent technologies high sensitivity dna bioanalyzer kit
    Schematic of the study. (A) )] were harvested by trypsinization and resuspended in medium, as indicated. Cell samples were lysed, generating crude nuclei preparations. Next, the transposition reaction was performed in the presence of Tn5 transposase, which cuts and ligates adapters at <t>DNA</t> regions of increased accessibility. The quality of the transposed DNA fragment libraries was assessed using a <t>Bioanalyzer.</t> Libraries were sequenced, mapped to the genome, and processed bioinformatically, and accessible genomic regions were detected (peak calling). (B) Single-cell (SC) RNA-seq [gel bead-in-emulsion (GEM) Drop-seq] workflow. Following treatment with GnRH or vehicle, cultured LβT2 cells were harvested by trypsinization and resuspended in a PBS BSA-containing buffer. Cell samples were loaded on a microfluidic chip, combined with reagents and barcoded gel beads to form Gel beads in Emulsion (GEMs), and then mixed with oil. The resulting emulsions were collected and reverse transcribed in SC droplets. Oil was removed, and barcoded cDNA was amplified, purified, and subject to quality control (QC) assessment. Amplified cDNA was incorporated into libraries, which were subsequently subject to QC evaluation using Kapa, Bioanalyzer, and Qubit. Libraries were pooled for sequencing and demultiplexed for subsequent analyses.
    High Sensitivity Dna Bioanalyzer Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high sensitivity dna bioanalyzer kit/product/Agilent technologies
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    high sensitivity dna bioanalyzer kit - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    99
    Agilent technologies high sensitivity dna analysis kit
    Variant overlap between <t>DNA</t> from PBMCs vs. <t>FFPE</t> tissue. Overlap of variants called by GATK in the FFPE (red circles) and PBMC (green circles) samples from the four patients included in the matched sample study. a – d show Patients1–4, respectively
    High Sensitivity Dna Analysis Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high sensitivity dna analysis kit/product/Agilent technologies
    Average 99 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    high sensitivity dna analysis kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    ORF37’s DNase activity is required for efficient packaging of viral DNA and nuclear egress. (A) qPCR analysis of BamHI-digested total and encapsidated (DNase I-resistant) DNAs from 72-hpi 293L/wt and 293L/Q129H cells. When normalized to the total viral DNA, DNA encapsidation in 293L/Q129H cells was significantly reduced compared to the wt control; ***, P

    Journal: Journal of Virology

    Article Title: The DNase Activity of Kaposi’s Sarcoma-Associated Herpesvirus SOX Protein Serves an Important Role in Viral Genome Processing during Lytic Replication

    doi: 10.1128/JVI.01983-18

    Figure Lengend Snippet: ORF37’s DNase activity is required for efficient packaging of viral DNA and nuclear egress. (A) qPCR analysis of BamHI-digested total and encapsidated (DNase I-resistant) DNAs from 72-hpi 293L/wt and 293L/Q129H cells. When normalized to the total viral DNA, DNA encapsidation in 293L/Q129H cells was significantly reduced compared to the wt control; ***, P

    Article Snippet: The ChIP-Seq libraries were validated using a Bioanalyzer 2100 with a high-sensitivity DNA ChIP (Agilent) and quantified by qPCR (Kappa kit).

    Techniques: Activity Assay, Real-time Polymerase Chain Reaction

    Generation of ORF37-DNase-inactivated (Q129H) recombinant KSHV (the Q129H mutant) via homologous recombination. (A) Schematic diagram showing generation of the Q129H mutant, where amino acid residue 129 of ORF37 was point mutated to histidine by homologous recombination and GalK-Kan r counterselection. Insertion of the galK -Kan r cassette was confirmed by BamHI restriction fragment analysis and Southern hybridization with a GalK-specific probe, indicated in red (Probe). (B) EtBr-stained gel and Southern blot of BamHI-digested BAC16-wt (lane 2), an intermediate containing a galK -Kan r cassette (lane 3), and the Q129H mutant (lane 4). The Southern blot with a 32 P-labeled GalK probe showed the expected 2.6-kb band in the intermediate (lane 3). (C, a ) DNA sequencing of BAC Q129H confirmed the desired mutation in the ORF37 gene. ( b ) High-throughput sequencing reads of BAC Q129H confirmed mutation in the ORF37 gene. (D) GFP expression levels from HEK293L cells stably transfected with BAC16-wt or the Q129H DNAs post-hygromycin selection were imaged directly from the fixed cells by fluorescence microscopy. The green images show the expression of GFP, confirming the presence of BAC16-wt or the Q129H mutant, and the gray images are the corresponding bright-field images. (E) Comparison of expression levels of LANA, RTA, and ORF37 via Western blot analysis of uninduced and 48-hpi 293L/wt and 293L/Q129H cells confirmed the persistence and reactivation of both viruses. Staining of the same blot with GAPDH-specific antibody served as a loading control. (F, a ) Analysis of the host shutoff activity of the Q129H mutant using RNAs extracted from uninduced and 48-hpi 293L/wt and 293L/Q129H cells. RNA was resolved and subjected to Northern blotting and hybridization with a 32 P-labeled GFP probe, which confirmed that the Q129H mutant retained its ability to degrade the host mRNA. ( b ) Immunofluorescence analysis for RTA expression in 293L/wt and 293L/Q129H cells indicated almost all the cells underwent lytic reaction at 48 hpi. DIC, differential interference contrast. (G) Relative quantification of the viral mRNA levels from 48-hpi 293L/Q129H cells compared to the 293L/wt control using a custom real-time qPCR analysis of the KSHV genes in a 96-well format showed almost identical levels of all viral transcripts. The fold changes for the individual KSHV genes were calculated using the ΔΔ C T method normalized to GAPDH and relative to BAC16-wt, defined as 1.

    Journal: Journal of Virology

    Article Title: The DNase Activity of Kaposi’s Sarcoma-Associated Herpesvirus SOX Protein Serves an Important Role in Viral Genome Processing during Lytic Replication

    doi: 10.1128/JVI.01983-18

    Figure Lengend Snippet: Generation of ORF37-DNase-inactivated (Q129H) recombinant KSHV (the Q129H mutant) via homologous recombination. (A) Schematic diagram showing generation of the Q129H mutant, where amino acid residue 129 of ORF37 was point mutated to histidine by homologous recombination and GalK-Kan r counterselection. Insertion of the galK -Kan r cassette was confirmed by BamHI restriction fragment analysis and Southern hybridization with a GalK-specific probe, indicated in red (Probe). (B) EtBr-stained gel and Southern blot of BamHI-digested BAC16-wt (lane 2), an intermediate containing a galK -Kan r cassette (lane 3), and the Q129H mutant (lane 4). The Southern blot with a 32 P-labeled GalK probe showed the expected 2.6-kb band in the intermediate (lane 3). (C, a ) DNA sequencing of BAC Q129H confirmed the desired mutation in the ORF37 gene. ( b ) High-throughput sequencing reads of BAC Q129H confirmed mutation in the ORF37 gene. (D) GFP expression levels from HEK293L cells stably transfected with BAC16-wt or the Q129H DNAs post-hygromycin selection were imaged directly from the fixed cells by fluorescence microscopy. The green images show the expression of GFP, confirming the presence of BAC16-wt or the Q129H mutant, and the gray images are the corresponding bright-field images. (E) Comparison of expression levels of LANA, RTA, and ORF37 via Western blot analysis of uninduced and 48-hpi 293L/wt and 293L/Q129H cells confirmed the persistence and reactivation of both viruses. Staining of the same blot with GAPDH-specific antibody served as a loading control. (F, a ) Analysis of the host shutoff activity of the Q129H mutant using RNAs extracted from uninduced and 48-hpi 293L/wt and 293L/Q129H cells. RNA was resolved and subjected to Northern blotting and hybridization with a 32 P-labeled GFP probe, which confirmed that the Q129H mutant retained its ability to degrade the host mRNA. ( b ) Immunofluorescence analysis for RTA expression in 293L/wt and 293L/Q129H cells indicated almost all the cells underwent lytic reaction at 48 hpi. DIC, differential interference contrast. (G) Relative quantification of the viral mRNA levels from 48-hpi 293L/Q129H cells compared to the 293L/wt control using a custom real-time qPCR analysis of the KSHV genes in a 96-well format showed almost identical levels of all viral transcripts. The fold changes for the individual KSHV genes were calculated using the ΔΔ C T method normalized to GAPDH and relative to BAC16-wt, defined as 1.

    Article Snippet: The ChIP-Seq libraries were validated using a Bioanalyzer 2100 with a high-sensitivity DNA ChIP (Agilent) and quantified by qPCR (Kappa kit).

    Techniques: Recombinant, Mutagenesis, Homologous Recombination, Hybridization, Staining, Southern Blot, Labeling, DNA Sequencing, BAC Assay, Next-Generation Sequencing, Expressing, Stable Transfection, Transfection, Selection, Fluorescence, Microscopy, Western Blot, Activity Assay, Northern Blot, Immunofluorescence, Real-time Polymerase Chain Reaction

    DNase activity of ORF37 did not affect latent/lytic KSHV DNA synthesis but rather virion production. (A and B) Comparison of intracellular KSHV genome copies in latent/uninduced and 48-hpi 293L/wt and 293L/Q129H cells, as analyzed by qPCR. The values were normalized to GAPDH and calculated relative to BAC16-wt, defined as 1. Equal numbers of KSHV genome copies were maintained during the latent phase; however, only a slight decrease in KSHV genome copies was observed during the lytic phase of virus replication. (C) Detection of extracellular KSHV progeny virion production in the supernatants of 5-dpi 293L/wt and 293L/Q129H cells revealed a 10-fold decrease in virus production in 293L/Q129H supernatants compared to wt supernatants. (D and E) HEK293L cells infected with 293L/wt and 293L/Q129H virus supernatants were assayed by flow cytometry 2 days postinfection. The side scatter (SSC) versus forward scatter (FSC) plot was used to identify GFP-positive cells/signals. (F) Mean value of at least three independent experiments from the values determined in panel E. The error bars represent standard deviations of the mean from the results of three different experiments; ***, P

    Journal: Journal of Virology

    Article Title: The DNase Activity of Kaposi’s Sarcoma-Associated Herpesvirus SOX Protein Serves an Important Role in Viral Genome Processing during Lytic Replication

    doi: 10.1128/JVI.01983-18

    Figure Lengend Snippet: DNase activity of ORF37 did not affect latent/lytic KSHV DNA synthesis but rather virion production. (A and B) Comparison of intracellular KSHV genome copies in latent/uninduced and 48-hpi 293L/wt and 293L/Q129H cells, as analyzed by qPCR. The values were normalized to GAPDH and calculated relative to BAC16-wt, defined as 1. Equal numbers of KSHV genome copies were maintained during the latent phase; however, only a slight decrease in KSHV genome copies was observed during the lytic phase of virus replication. (C) Detection of extracellular KSHV progeny virion production in the supernatants of 5-dpi 293L/wt and 293L/Q129H cells revealed a 10-fold decrease in virus production in 293L/Q129H supernatants compared to wt supernatants. (D and E) HEK293L cells infected with 293L/wt and 293L/Q129H virus supernatants were assayed by flow cytometry 2 days postinfection. The side scatter (SSC) versus forward scatter (FSC) plot was used to identify GFP-positive cells/signals. (F) Mean value of at least three independent experiments from the values determined in panel E. The error bars represent standard deviations of the mean from the results of three different experiments; ***, P

    Article Snippet: The ChIP-Seq libraries were validated using a Bioanalyzer 2100 with a high-sensitivity DNA ChIP (Agilent) and quantified by qPCR (Kappa kit).

    Techniques: Activity Assay, DNA Synthesis, Real-time Polymerase Chain Reaction, Infection, Flow Cytometry, Cytometry

    Amplification and cloning of sg mRNA leader–body junctions generated from additional functional TRSs located in the genomic region between identified TRS5 and TRS6. ( A ) Diagram indicating the positions of the primers used and the estimated size for the amplified leader–body junction sequences (thick black line). The white open box represents ORF5, and the short black box represents the leader sequence in transcribed sg mRNAs. ( B ) MA104 cells were either mock-infected (M) or infected with SHFVic at an MOI of 1. At 24 hpi, total intracellular RNA was extracted and subjected to RT-PCR, and the products were separated on a 2% DNA gel. The band with the size estimated for the leader–body junction in sg mRNA5 produced from the known TRS5 is indicated by an arrow. PCR bands with sizes estimated for the leader–body junctions of ∼1.7-kb sg mRNAs are indicated by a bracket. L, ladder. ( C ) The bracketed region of the gel was excised, and the DNA was extracted and cloned into a TA vector. Forty colonies were randomly selected and subjected to restriction digestion, and the inserts were separated by gel electrophoresis. The results from 10 representative clones are shown. L, ladder. ( D ) Diagram showing the locations of the known and previously unreported body TRSs. The TRSs are indicated by black vertical bars. The previously unreported functional TRSs are within a dotted line box. The ORFs encoded by the individual sg mRNAs are indicated by white open boxes. 5-C, ORF5-C-68aa; L, leader region.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Expanded subgenomic mRNA transcriptome and coding capacity of a nidovirus

    doi: 10.1073/pnas.1706696114

    Figure Lengend Snippet: Amplification and cloning of sg mRNA leader–body junctions generated from additional functional TRSs located in the genomic region between identified TRS5 and TRS6. ( A ) Diagram indicating the positions of the primers used and the estimated size for the amplified leader–body junction sequences (thick black line). The white open box represents ORF5, and the short black box represents the leader sequence in transcribed sg mRNAs. ( B ) MA104 cells were either mock-infected (M) or infected with SHFVic at an MOI of 1. At 24 hpi, total intracellular RNA was extracted and subjected to RT-PCR, and the products were separated on a 2% DNA gel. The band with the size estimated for the leader–body junction in sg mRNA5 produced from the known TRS5 is indicated by an arrow. PCR bands with sizes estimated for the leader–body junctions of ∼1.7-kb sg mRNAs are indicated by a bracket. L, ladder. ( C ) The bracketed region of the gel was excised, and the DNA was extracted and cloned into a TA vector. Forty colonies were randomly selected and subjected to restriction digestion, and the inserts were separated by gel electrophoresis. The results from 10 representative clones are shown. L, ladder. ( D ) Diagram showing the locations of the known and previously unreported body TRSs. The TRSs are indicated by black vertical bars. The previously unreported functional TRSs are within a dotted line box. The ORFs encoded by the individual sg mRNAs are indicated by white open boxes. 5-C, ORF5-C-68aa; L, leader region.

    Article Snippet: The final dsDNA library was validated with an Agilent High Sensitivity DNA Kit (Agilent Technologies) and subjected to RNA-seq using Illumina HiSeq (paired-end reads, 100-bp read length) with one sample per lane.

    Techniques: Amplification, Clone Assay, Generated, Functional Assay, Sequencing, Infection, Reverse Transcription Polymerase Chain Reaction, Produced, Polymerase Chain Reaction, Plasmid Preparation, Nucleic Acid Electrophoresis

    Schematic of the study. (A) )] were harvested by trypsinization and resuspended in medium, as indicated. Cell samples were lysed, generating crude nuclei preparations. Next, the transposition reaction was performed in the presence of Tn5 transposase, which cuts and ligates adapters at DNA regions of increased accessibility. The quality of the transposed DNA fragment libraries was assessed using a Bioanalyzer. Libraries were sequenced, mapped to the genome, and processed bioinformatically, and accessible genomic regions were detected (peak calling). (B) Single-cell (SC) RNA-seq [gel bead-in-emulsion (GEM) Drop-seq] workflow. Following treatment with GnRH or vehicle, cultured LβT2 cells were harvested by trypsinization and resuspended in a PBS BSA-containing buffer. Cell samples were loaded on a microfluidic chip, combined with reagents and barcoded gel beads to form Gel beads in Emulsion (GEMs), and then mixed with oil. The resulting emulsions were collected and reverse transcribed in SC droplets. Oil was removed, and barcoded cDNA was amplified, purified, and subject to quality control (QC) assessment. Amplified cDNA was incorporated into libraries, which were subsequently subject to QC evaluation using Kapa, Bioanalyzer, and Qubit. Libraries were pooled for sequencing and demultiplexed for subsequent analyses.

    Journal: Frontiers in Endocrinology

    Article Title: Regulatory Architecture of the LβT2 Gonadotrope Cell Underlying the Response to Gonadotropin-Releasing Hormone

    doi: 10.3389/fendo.2018.00034

    Figure Lengend Snippet: Schematic of the study. (A) )] were harvested by trypsinization and resuspended in medium, as indicated. Cell samples were lysed, generating crude nuclei preparations. Next, the transposition reaction was performed in the presence of Tn5 transposase, which cuts and ligates adapters at DNA regions of increased accessibility. The quality of the transposed DNA fragment libraries was assessed using a Bioanalyzer. Libraries were sequenced, mapped to the genome, and processed bioinformatically, and accessible genomic regions were detected (peak calling). (B) Single-cell (SC) RNA-seq [gel bead-in-emulsion (GEM) Drop-seq] workflow. Following treatment with GnRH or vehicle, cultured LβT2 cells were harvested by trypsinization and resuspended in a PBS BSA-containing buffer. Cell samples were loaded on a microfluidic chip, combined with reagents and barcoded gel beads to form Gel beads in Emulsion (GEMs), and then mixed with oil. The resulting emulsions were collected and reverse transcribed in SC droplets. Oil was removed, and barcoded cDNA was amplified, purified, and subject to quality control (QC) assessment. Amplified cDNA was incorporated into libraries, which were subsequently subject to QC evaluation using Kapa, Bioanalyzer, and Qubit. Libraries were pooled for sequencing and demultiplexed for subsequent analyses.

    Article Snippet: Library QC and quantification were assessed using Nanodrop, Qubit (fluorometric quantitation, ThermoFisher Scientific), Kapa (quantification, Kapa Biosystems), High-Sensitivity DNA Bioanalyzer kit (Agilent), and qPCR of selected genes.

    Techniques: RNA Sequencing Assay, Cell Culture, Chromatin Immunoprecipitation, Amplification, Purification, Sequencing

    Variant overlap between DNA from PBMCs vs. FFPE tissue. Overlap of variants called by GATK in the FFPE (red circles) and PBMC (green circles) samples from the four patients included in the matched sample study. a – d show Patients1–4, respectively

    Journal: NPJ Genomic Medicine

    Article Title: A robust targeted sequencing approach for low input and variable quality DNA from clinical samples

    doi: 10.1038/s41525-017-0041-4

    Figure Lengend Snippet: Variant overlap between DNA from PBMCs vs. FFPE tissue. Overlap of variants called by GATK in the FFPE (red circles) and PBMC (green circles) samples from the four patients included in the matched sample study. a – d show Patients1–4, respectively

    Article Snippet: To obtain an idea of the degree of fragmentation in FFPE-derived gDNA samples prior to sonication, HD200 and two clinical FFPE samples were analyzed by a High Sensitivity DNA analysis kit on a Bioanalyzer 2100 (Agilent, Santa Clara, CA) (Supplementary Figure ).

    Techniques: Variant Assay, Formalin-fixed Paraffin-Embedded