high resolution nano hplc tandem mass spectrometry analysis  (Thermo Fisher)


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    Thermo Fisher high resolution nano hplc tandem mass spectrometry analysis
    Scheme of label-free quantitative proteomics. Synchronized animals at L4 stage were either fed E. Coli OP50 (uninfected controls) or P. aeruginosa PA14 (infected samples) for 4 hr, collected, and lysed. Total proteins were extracted and digested with trypsin. Peptides from control or infected samples were subject to <t>nano-HPLC</t> tandem MS analysis. Quantification is based on the comparison of peak intensity of same peptides in different samples.
    High Resolution Nano Hplc Tandem Mass Spectrometry Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high resolution nano hplc tandem mass spectrometry analysis/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high resolution nano hplc tandem mass spectrometry analysis - by Bioz Stars, 2020-04
    91/100 stars

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    1) Product Images from "Neuronal GPCR OCTR-1 regulates innate immunity by controlling protein synthesis in Caenorhabditis elegans"

    Article Title: Neuronal GPCR OCTR-1 regulates innate immunity by controlling protein synthesis in Caenorhabditis elegans

    Journal: Scientific Reports

    doi: 10.1038/srep36832

    Scheme of label-free quantitative proteomics. Synchronized animals at L4 stage were either fed E. Coli OP50 (uninfected controls) or P. aeruginosa PA14 (infected samples) for 4 hr, collected, and lysed. Total proteins were extracted and digested with trypsin. Peptides from control or infected samples were subject to nano-HPLC tandem MS analysis. Quantification is based on the comparison of peak intensity of same peptides in different samples.
    Figure Legend Snippet: Scheme of label-free quantitative proteomics. Synchronized animals at L4 stage were either fed E. Coli OP50 (uninfected controls) or P. aeruginosa PA14 (infected samples) for 4 hr, collected, and lysed. Total proteins were extracted and digested with trypsin. Peptides from control or infected samples were subject to nano-HPLC tandem MS analysis. Quantification is based on the comparison of peak intensity of same peptides in different samples.

    Techniques Used: Infection, High Performance Liquid Chromatography, Mass Spectrometry

    Related Articles

    Isolation:

    Article Title: Neuronal GPCR OCTR-1 regulates innate immunity by controlling protein synthesis in Caenorhabditis elegans
    Article Snippet: High resolution nano-HPLC tandem mass spectrometry analysis The peptide samples were subjected to Thermo Scientific Orbitrap Fusion Tribrid with an Easy-nLC 1000 ultra-high pressure LC on a Thermo Scientific PepMap 100 C18 column (2 μm, 50 μm × 15 cm). .. The run was completed by holding a 97% B for 10 min. MS1 data was acquired on an Orbitrap Fusion mass spectrometry using a full scan method according to the following parameters: scan range 400–1500 m/z, Orbitrap resolution 120,000; AGC target 400,000; and maximum injection time of 50 ms. MS2 data were collected using the following parameters: rapid scan rate, HCD collision energy 35%, 1.6 m/z isolation window, AGC 2,000 and maximum injection time of 50 ms. MS2 precursors were selected for a 3 s cycle.

    Injection:

    Article Title: Neuronal GPCR OCTR-1 regulates innate immunity by controlling protein synthesis in Caenorhabditis elegans
    Article Snippet: High resolution nano-HPLC tandem mass spectrometry analysis The peptide samples were subjected to Thermo Scientific Orbitrap Fusion Tribrid with an Easy-nLC 1000 ultra-high pressure LC on a Thermo Scientific PepMap 100 C18 column (2 μm, 50 μm × 15 cm). .. The run was completed by holding a 97% B for 10 min. MS1 data was acquired on an Orbitrap Fusion mass spectrometry using a full scan method according to the following parameters: scan range 400–1500 m/z, Orbitrap resolution 120,000; AGC target 400,000; and maximum injection time of 50 ms. MS2 data were collected using the following parameters: rapid scan rate, HCD collision energy 35%, 1.6 m/z isolation window, AGC 2,000 and maximum injection time of 50 ms. MS2 precursors were selected for a 3 s cycle.

    Mass Spectrometry:

    Article Title: Neuronal GPCR OCTR-1 regulates innate immunity by controlling protein synthesis in Caenorhabditis elegans
    Article Snippet: .. High resolution nano-HPLC tandem mass spectrometry analysis The peptide samples were subjected to Thermo Scientific Orbitrap Fusion Tribrid with an Easy-nLC 1000 ultra-high pressure LC on a Thermo Scientific PepMap 100 C18 column (2 μm, 50 μm × 15 cm). ..

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  • 91
    Thermo Fisher high resolution nano hplc tandem mass spectrometry analysis
    Scheme of label-free quantitative proteomics. Synchronized animals at L4 stage were either fed E. Coli OP50 (uninfected controls) or P. aeruginosa PA14 (infected samples) for 4 hr, collected, and lysed. Total proteins were extracted and digested with trypsin. Peptides from control or infected samples were subject to <t>nano-HPLC</t> tandem MS analysis. Quantification is based on the comparison of peak intensity of same peptides in different samples.
    High Resolution Nano Hplc Tandem Mass Spectrometry Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high resolution nano hplc tandem mass spectrometry analysis/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high resolution nano hplc tandem mass spectrometry analysis - by Bioz Stars, 2020-04
    91/100 stars
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    Scheme of label-free quantitative proteomics. Synchronized animals at L4 stage were either fed E. Coli OP50 (uninfected controls) or P. aeruginosa PA14 (infected samples) for 4 hr, collected, and lysed. Total proteins were extracted and digested with trypsin. Peptides from control or infected samples were subject to nano-HPLC tandem MS analysis. Quantification is based on the comparison of peak intensity of same peptides in different samples.

    Journal: Scientific Reports

    Article Title: Neuronal GPCR OCTR-1 regulates innate immunity by controlling protein synthesis in Caenorhabditis elegans

    doi: 10.1038/srep36832

    Figure Lengend Snippet: Scheme of label-free quantitative proteomics. Synchronized animals at L4 stage were either fed E. Coli OP50 (uninfected controls) or P. aeruginosa PA14 (infected samples) for 4 hr, collected, and lysed. Total proteins were extracted and digested with trypsin. Peptides from control or infected samples were subject to nano-HPLC tandem MS analysis. Quantification is based on the comparison of peak intensity of same peptides in different samples.

    Article Snippet: High resolution nano-HPLC tandem mass spectrometry analysis The peptide samples were subjected to Thermo Scientific Orbitrap Fusion Tribrid with an Easy-nLC 1000 ultra-high pressure LC on a Thermo Scientific PepMap 100 C18 column (2 μm, 50 μm × 15 cm).

    Techniques: Infection, High Performance Liquid Chromatography, Mass Spectrometry