high quality taq polymerase  (Qiagen)

 
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    Name:
    HotStar HiFidelity Polymerase Kit
    Description:
    For highly sensitive and reliable high fidelity hot start PCR Kit contents Qiagen HotStar HiFidelity Polymerase Kit 100U Single Genomic DNA and cDNA Sample PCR Amplification Proofreading Reaction type 3 5 Exonuclease Enzyme Activity A addition Enzyme Activity Ideal for Cloning Mutation Analysis TA or UA cloning Procedures For Highly Sensitive and Reliable High fidelity Hot start PCR Includes HotStar HiFidelity DNA Polymerase 5x HotStar HiFidelity PCR Buffer incl dNTPs 5x Q Solution 25mM MgSO4 RNase free Water Benefits High sensitivity and specificity Unique UA TA cloning feature Fidelity 10 fold higher than Taq DNA Polymerase Room temperature setup and fast enzyme activation
    Catalog Number:
    202602
    Price:
    134
    Category:
    HotStar HiFidelity Polymerase Kit
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    Structured Review

    Qiagen high quality taq polymerase
    HotStar HiFidelity Polymerase Kit
    For highly sensitive and reliable high fidelity hot start PCR Kit contents Qiagen HotStar HiFidelity Polymerase Kit 100U Single Genomic DNA and cDNA Sample PCR Amplification Proofreading Reaction type 3 5 Exonuclease Enzyme Activity A addition Enzyme Activity Ideal for Cloning Mutation Analysis TA or UA cloning Procedures For Highly Sensitive and Reliable High fidelity Hot start PCR Includes HotStar HiFidelity DNA Polymerase 5x HotStar HiFidelity PCR Buffer incl dNTPs 5x Q Solution 25mM MgSO4 RNase free Water Benefits High sensitivity and specificity Unique UA TA cloning feature Fidelity 10 fold higher than Taq DNA Polymerase Room temperature setup and fast enzyme activation
    https://www.bioz.com/result/high quality taq polymerase/product/Qiagen
    Average 86 stars, based on 38823 article reviews
    Price from $9.99 to $1999.99
    high quality taq polymerase - by Bioz Stars, 2020-09
    86/100 stars

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    Related Articles

    Sequencing:

    Article Title: Phosphorylation of Sterol Regulatory Element Binding Protein-1a by Protein Kinase A (PKA) Regulates Transcriptional Activity
    Article Snippet: .. The coding sequence was amplified by PCR from rat hepatocyte cDNA using HotStar HiFidelity Taq Polymerase kit (Qiagen). ..

    Article Title: A Transposon in Comt Generates mRNA Variants and Causes Widespread Expression and Behavioral Differences among Mice
    Article Snippet: .. DNA amplification was performed using the HotStar HiFidelity Polymerase kit (Qiagen) and 10 ng of genomic DNA for each strain followed by sequencing of the PCR products with each custom primer using an A3130XL Genetic Analyzer (Applied Biosystems). ..

    Polymerase Chain Reaction:

    Article Title: Phosphorylation of Sterol Regulatory Element Binding Protein-1a by Protein Kinase A (PKA) Regulates Transcriptional Activity
    Article Snippet: .. The coding sequence was amplified by PCR from rat hepatocyte cDNA using HotStar HiFidelity Taq Polymerase kit (Qiagen). ..

    Article Title: ZFP191 is required by oligodendrocytes for CNS myelination
    Article Snippet: .. Full-length Zfp191 was amplified from wild-type mouse cDNA using the HotStar HiFidelity PCR kit (Qiagen). .. The primers used for the full-length sequence were GTCCCGAGCCAGAGGAGTA and CACAACATTCAAAAGTTTTTCTGC.

    Article Title: A Transposon in Comt Generates mRNA Variants and Causes Widespread Expression and Behavioral Differences among Mice
    Article Snippet: .. DNA amplification was performed using the HotStar HiFidelity Polymerase kit (Qiagen) and 10 ng of genomic DNA for each strain followed by sequencing of the PCR products with each custom primer using an A3130XL Genetic Analyzer (Applied Biosystems). ..

    Article Title: Identification of single nucleotide polymorphisms in Toll-like receptor candidate genes associated with tuberculosis infection in water buffalo (Bubalus bubalis)
    Article Snippet: .. PCR reactions were performed with the HotStar HiFidelity Polymerase Kit (Qiagen), and the reaction mixture included 50 ng of genomic DNA and 0.5 μM of each primer (forward and reverse) in a final volume of 25 μl. .. Amplicons were purified (QiaQuick purification kit, Qiagen), bi-directionally sequenced using the Big Dye Terminator cycle sequencing kit v.1.1 or v.3.1 (Life Technologies) and purified using the DyeEx spin kit (Qiagen).

    Amplification:

    Article Title: Phosphorylation of Sterol Regulatory Element Binding Protein-1a by Protein Kinase A (PKA) Regulates Transcriptional Activity
    Article Snippet: .. The coding sequence was amplified by PCR from rat hepatocyte cDNA using HotStar HiFidelity Taq Polymerase kit (Qiagen). ..

    Article Title: ZFP191 is required by oligodendrocytes for CNS myelination
    Article Snippet: .. Full-length Zfp191 was amplified from wild-type mouse cDNA using the HotStar HiFidelity PCR kit (Qiagen). .. The primers used for the full-length sequence were GTCCCGAGCCAGAGGAGTA and CACAACATTCAAAAGTTTTTCTGC.

    Article Title: A Transposon in Comt Generates mRNA Variants and Causes Widespread Expression and Behavioral Differences among Mice
    Article Snippet: .. DNA amplification was performed using the HotStar HiFidelity Polymerase kit (Qiagen) and 10 ng of genomic DNA for each strain followed by sequencing of the PCR products with each custom primer using an A3130XL Genetic Analyzer (Applied Biosystems). ..

    Article Title: Transcriptional changes in response to X chromosome dosage in the mouse: implications for X inactivation and the molecular basis of Turner Syndrome
    Article Snippet: .. Typically 50 ng of DNA and 100 ng of cDNA were amplified using the HotStar HiFidelity Polymerase Kit (Qiagen) in a 10 μl reaction volume comprising 0.3 μM of each primer. .. PCR conditions were the following: 15 min pre-incubation step at 94°C, 35 cycles of denaturation at 94°C for 30 sec, annealing for 45 sec at the respective AT for each primer pair and extension at 72°C for 45 sec, followed by a final extension step at 72°C for 10 min.

    Mutagenesis:

    Article Title: LIV-1 ZIP Ectodomain Shedding in Prion-Infected Mice Resembles Cellular Response to Transition Metal Starvation
    Article Snippet: .. The HotStar HiFidelity polymerase (Qiagen, Valencia, CA, USA) was used for site-directed mutagenesis. .. A KLH-conjugated peptide derived from mouse ZIP10 (CNHDHSEQYEHNR) was synthesized and injected into Charles River Laboratories SPF rabbits (Division of Comparative Medicine, University of Toronto).

    DNA Ligation:

    Article Title: Seipin Is a Discrete Homooligomer †
    Article Snippet: .. Molecular biology enzymes were purchased from New England Biolabs, the rapid DNA ligation kit was from Roche, and the HotStar HiFidelity polymerase kit was from Qiagen. .. Sephacryl S-500 high resolution was from GE Healthcare, and dextran from Leuconostoc mesenteroides (MW 5–40 MDa) was from Sigma.

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  • Bioz Stars
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  • 86
    Qiagen high quality taq polymerase
    High Quality Taq Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high quality taq polymerase/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high quality taq polymerase - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    99
    Qiagen high quality taq polymerases qiagen taq dna polymerase
    High Quality Taq Polymerases Qiagen Taq Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high quality taq polymerases qiagen taq dna polymerase/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high quality taq polymerases qiagen taq dna polymerase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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