high quality taq polymerases qiagen taq dna polymerase  (Qiagen)


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    Name:
    Taq DNA Polymerase
    Description:
    Qiagen Taq DNA Polymerase, 250U, 5U/L, 10 min. at 97C; 60 min. at 94C Half-life, 2 to 4 kb/min. at 72C Extension Rate, Genomic DNA and cDNA Sample, PCR Amplification, Exonuclease Enzyme Activity, Minimal Optimization, Ideal for Standard and Specialized PCR Applications, Includes Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25mM MgCl2
    Catalog Number:
    201203
    Price:
    None
    Score:
    85
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    Structured Review

    Qiagen high quality taq polymerases qiagen taq dna polymerase
    Qiagen Taq DNA Polymerase, 250U, 5U/L, 10 min. at 97C; 60 min. at 94C Half-life, 2 to 4 kb/min. at 72C Extension Rate, Genomic DNA and cDNA Sample, PCR Amplification, Exonuclease Enzyme Activity, Minimal Optimization, Ideal for Standard and Specialized PCR Applications, Includes Taq DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25mM MgCl2
    https://www.bioz.com/result/high quality taq polymerases qiagen taq dna polymerase/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high quality taq polymerases qiagen taq dna polymerase - by Bioz Stars, 2019-12
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    Related Articles

    Clone Assay:

    Article Title: Ancestral Gene Organization in the Mitochondrial Genome of Thyridosmylus langii (McLachlan, 1870) (Neuroptera: Osmylidae) and Implications for Lacewing Evolution
    Article Snippet: Paragraph title: PCR amplification, cloning and sequencing ... Short PCRs were conducted using Qiagen Taq DNA polymerase (Qiagen, Beijing, China) with the following cycling conditions: 5 min at 94°C, followed by 35 cycles of 50 s at 94°C, 30 s at 40–55°C, and 1–2 min at 72°C.

    Article Title: Members of a Large Retroposon Family Are Determinants of Post-Transcriptional Gene Expression in Leishmania
    Article Snippet: The LUC -chimeric mRNAs transcribed from this vector are processed in Leishmania using sequences within the alpha-tubulin intergenic region cloned at the 5′-end. .. The full-length 3′UTR of LmjF36.3810 and LmjF08.1270 transcripts from the termination codon to 434 bp beyond the poly(A) site in the case of LmjF36.3810, and to 84 bp beyond the poly(A) site in the case of LmjF08.1270, or the LmSIDER2 element or the 3′UTR lacking LmSIDER2, were amplified by PCR using Taq DNA polymerase (Qiagen, http://www.qiagen.com/ ) and primers with inserted BamHI or PstI restriction sites (see ).

    Article Title: Knockdown resistance in Anopheles vagus, An. sinensis, An. paraliae and An. peditaeniatus populations of the Mekong region
    Article Snippet: The resulting PCR product was cloned by use of the Original TA cloning kit according to the manufacturer's instructions (Invitrogen, Carlsbad, California). .. All AS-PCR assays were performed in a 50 μl reaction mixture containing: 1 × Qiagen PCR buffer, 1 × Q solution, 0.5 mM MgCl2 , 200 μM dNTP's, 400 nM of the outer forward and reverse primer, 500 nM of the inner resistant and sensitive primer (Figure ), 1 unit Taq DNA polymerase (Qiagen, Hilden, Germany) and 1 μl template.

    Amplification:

    Article Title: Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Allelic Variants Relate to Shifts in Faecal Microbiota of Cystic Fibrosis Patients
    Article Snippet: Paragraph title: PCR Amplification of Bacterial Gene Coding for 16S rRNA ... PCR was performed with Taq DNA-polymerase (Hot Star Taq Plus, QIAGEN).

    Article Title: Burkholderia cenocepacia conditional growth mutant library created by random promoter replacement of essential genes
    Article Snippet: Conjugation into B. cenocepacia K56-2 was accomplished by triparental mating (Craig et al. ) with E. coli DH5α carrying the helper plasmid pRK2013 (Figurski and Helinski ). .. DNA was amplified using a PTC-221 DNA engine (MJ Research, Waltham, MA) or an Eppendorf Mastercycler ep gradient S thermal cycler with either Taq DNA polymerase (Qiagen, Hilden, Germany) or Phusion High-Fidelity PCR Kit (New England Biolabs). .. Amplification conditions were optimized for each primer pair.

    Article Title: Ancestral Gene Organization in the Mitochondrial Genome of Thyridosmylus langii (McLachlan, 1870) (Neuroptera: Osmylidae) and Implications for Lacewing Evolution
    Article Snippet: Paragraph title: PCR amplification, cloning and sequencing ... Short PCRs were conducted using Qiagen Taq DNA polymerase (Qiagen, Beijing, China) with the following cycling conditions: 5 min at 94°C, followed by 35 cycles of 50 s at 94°C, 30 s at 40–55°C, and 1–2 min at 72°C.

    Article Title: Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans
    Article Snippet: Extracted genomic DNA was quantified using a NanoDrop ND1000 spectrophotometer (Peqlab; Erlangen, Germany). .. PCR amplification of genomic DNA was carried out using the HotMasterMix 2.5x from 5 PRIME (Hamburg, Germany) according to the manufacturer’s protocol or the Taq DNA polymerase from Qiagen (Hilden, Germany) in reaction buffer containing 200 μM (each) deoxynucleotide triphosphates (dNTPs), 1 μM (each) oligonucleotide primers and ca. .. 10 – 25 ng of genomic DNA in a final volume of 20 μl.

    Article Title: Identification of Key Uric Acid Synthesis Pathway in a Unique Mutant Silkworm Bombyx mori Model of Parkinson's Disease
    Article Snippet: Protein concentrations were determined using a Bradford assay kit (Pierce, Rockford, IL, USA), according to the manufacturer’s instructions. .. Total RNA from 500 brains of day 3–5 fifth instar larvae were treated with DNase and processed for cDNA synthesis using primers of 12 to 18 oligo(dT) and SuperScript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA). cDNA was amplified by PCR using Taq DNA polymerase (Qiagen) with the primers for tyrosine hydroxylase (TH; 5′-ACACAACAGTGGTTCAGTCG-3′ and 5′-AGGAGTCTCGCAATGTACAC-3′ ), actin ( 5′-TATCGCCGACAGGATGCAGAAGGA-3′ and 5′-TAGAAGCACTTGCGGTGAACGATG-3′ ) and DJ-1 ( 5′-CATTTGTGCTGCTTCCATAGCGTT-3′ and 5′-CATTCCCTTTTCGACTTGATCGGC-3′ ), as described previously . .. RT-PCR for B. mori actin was used as a positive loading control.

    Article Title: Identification of More Than Two Paternal Haplotypes of the Ovine Fatty Acid-Binding Protein 4 (FABP4) Gene in Half-Sib Families: Evidence of Intragenic Meiotic Recombination
    Article Snippet: Briefly, region 1 (exon 2 - intron 2) was amplified using the PCR primers 5′-tgtgggctttgctaccag-3′ and 5′-taaatgggagacaattcacc-3′, while region 2 (exon 3 - intron 3) was amplified using the primers 5′-acttagatgaaggtgctctg-3′ and 5′- ctcaggactaaacaactcatg-3′. .. Amplifications were performed in a 15-µL reaction containing the DNA on one 1.2 mm punch of FTA card, 0.25 µM of each paired primer set, 150 µM dNTPs (Bioline, London, UK), 2.5 mM Mg2+ , 0.5 U Taq DNA polymerase (Qiagen, Hilden, Germany) and 1× the reaction buffer supplied with the enzyme.

    Article Title: The invasive New Guinea flatworm Platydemus manokwari in France, the first record for Europe: time for action is now
    Article Snippet: A fragment of 424 bp of COI gene was amplified with the primers COI-ASmit1 (forward 5′-TTTTTTGGGCATCCTGAGGTTTAT-3′) and COI-ASmit2 (reverse 5′-TAAAGAAAGAACATAATGAAAATG-3′) ( ). .. The PCR reaction was performed in 20 µl, containing 1 ng of DNA, 1× CoralLoad PCR buffer, 3 Mm MgCl2, 66 µM of each dNTP, 0.15 µM of each primer, and 0.5 units of Taq DNA polymerase (Qiagen).

    Article Title: Silencing and Un-silencing of Tetracycline-Controlled Genes in Neurons
    Article Snippet: Paragraph title: Sodium bisulphite conversion, PCR amplification and sequencing ... PCRs were performed on MJ Research thermocyclers (Waltham, Massachusetts, United States) in a final volume of 25 µl containing 1× PCR Buffer, 1 U /Taq/ DNA polymerase (Qiagen), 200 µM dNTPs, 12.5 pmol each of forward and reverse primers, and 7 ng of bisulphite-treated genomic DNA.

    Article Title: Faithful transcription initiation from a mitochondrial promoter in transgenic plastids
    Article Snippet: 5′ termini of in vivo synthesized transcripts were determined employing a 5′-RACE technique combined with tobacco acid pyrophosphatase (TAP) treatment as described by Kühn et al. ( ) with the following modifications: The gene-specific primer used for reverse transcription was Pa (5′-CTGTTGTGCCCAGTCATAG-3′). .. The products of reverse transcription (RT) were amplified in a first PCR step by using 4 µl of the RT reaction, 5 pmol of both the adapter-specific forward primer P1a (5′-CGAATTCCTGTAGAACGAACACTAGAAG-3′) and the gene-specific reverse primer Pb (5′-ATAGTTTGTGGAGAATTTCGCTTCC-3′), 200 µM of each dNTP and 0.5 U of Taq DNA polymerase (Qiagen) in 25 µl of the appropriate buffer. .. Amplification conditions comprised an initial denaturation step at 94°C for 1 min followed by 35 cycles of 95°C for 20 s, 58°C for 20 s and 72°C for 2 min and a final extension step at 72°C for 10 min. A 1 µl aliquot of the first PCR reaction was used as template for subsequent nested PCRs set up essentially as the first PCR in a volume of 50 µl with 10 pmol of both the gene-specific primer Pc (5′-CTGTGTTAAGCATAGGGCCTAACTAGC′3′) and the adapter-specific primer P1a.

    Article Title: Members of a Large Retroposon Family Are Determinants of Post-Transcriptional Gene Expression in Leishmania
    Article Snippet: The different LUC -chimeric constructs listed in were made as follows. .. The full-length 3′UTR of LmjF36.3810 and LmjF08.1270 transcripts from the termination codon to 434 bp beyond the poly(A) site in the case of LmjF36.3810, and to 84 bp beyond the poly(A) site in the case of LmjF08.1270, or the LmSIDER2 element or the 3′UTR lacking LmSIDER2, were amplified by PCR using Taq DNA polymerase (Qiagen, http://www.qiagen.com/ ) and primers with inserted BamHI or PstI restriction sites (see ). .. PCR products were cloned into vector pCR2.1 (Invitrogen, http://www.invitrogen.com/ ), digested with BamHI or PstI (New England Biolabs, http://www.neb.com/ ) and subcloned into the BamHI site downstream of the LUC gene in vector pSPYNEOαLUC [ ].

    Article Title: Impairment of germline transmission after blastocyst injection with murine embryonic stem cells cultured with mouse hepatitis virus and mouse minute virus
    Article Snippet: For PCR analysis, 1 μg (μl) cDNA was used for amplification. .. PCR was performed in a total volume of 20 μl using Taq DNA polymerase (Qiagen) for 35 cycles in a thermocycler (Biometra, Biomedizinische Analytik GmbH, Göttingen, Germany).

    Article Title: A specific insertion of a solo-LTR characterizes the Y-chromosome of Bryonia dioica (Cucurbitaceae)
    Article Snippet: Cycle conditions for the second PCR: 5 cycles of 25 s at 94°C, 3 min at 72°C, 20 cycles of 25 s at 94°C, 3 min at 67°C for amplification and 67°C for 10 min after the final cycle. .. All PCR mixtures contained 1.25 Units Taq DNA polymerase (QIAGEN GmbH, Hilden, Germany [Qiagen]), 1× Taq buffer, 15 mM MgCl2 , 10 mM of each dNTP and 10 pmol of each primer in a total volume of 50 μl.

    Article Title: Knockdown resistance in Anopheles vagus, An. sinensis, An. paraliae and An. peditaeniatus populations of the Mekong region
    Article Snippet: All AS-PCR assays were performed in a 50 μl reaction mixture containing: 1 × Qiagen PCR buffer, 1 × Q solution, 0.5 mM MgCl2 , 200 μM dNTP's, 400 nM of the outer forward and reverse primer, 500 nM of the inner resistant and sensitive primer (Figure ), 1 unit Taq DNA polymerase (Qiagen, Hilden, Germany) and 1 μl template. .. The cycling conditions described above for the amplification of the DIIS6 region were used.

    Filtration:

    Article Title: A specific insertion of a solo-LTR characterizes the Y-chromosome of Bryonia dioica (Cucurbitaceae)
    Article Snippet: All PCR mixtures contained 1.25 Units Taq DNA polymerase (QIAGEN GmbH, Hilden, Germany [Qiagen]), 1× Taq buffer, 15 mM MgCl2 , 10 mM of each dNTP and 10 pmol of each primer in a total volume of 50 μl. .. Amplicons were purified for sequencing and sequencing reactions were performed with the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, California [ABI]).

    Positive Control:

    Article Title: Knockdown resistance in Anopheles vagus, An. sinensis, An. paraliae and An. peditaeniatus populations of the Mekong region
    Article Snippet: All AS-PCR assays were performed in a 50 μl reaction mixture containing: 1 × Qiagen PCR buffer, 1 × Q solution, 0.5 mM MgCl2 , 200 μM dNTP's, 400 nM of the outer forward and reverse primer, 500 nM of the inner resistant and sensitive primer (Figure ), 1 unit Taq DNA polymerase (Qiagen, Hilden, Germany) and 1 μl template. .. The amplification products were electrophoresed on a 3% mixed agarose gel (1.5% agarose and 1.5% small fragment agarose) and visualised under UV light after ethidium bromide staining.

    Synthesized:

    Article Title: Faithful transcription initiation from a mitochondrial promoter in transgenic plastids
    Article Snippet: Paragraph title: 5′-end mapping of in vivo and in vitro -synthesized RNAs ... The products of reverse transcription (RT) were amplified in a first PCR step by using 4 µl of the RT reaction, 5 pmol of both the adapter-specific forward primer P1a (5′-CGAATTCCTGTAGAACGAACACTAGAAG-3′) and the gene-specific reverse primer Pb (5′-ATAGTTTGTGGAGAATTTCGCTTCC-3′), 200 µM of each dNTP and 0.5 U of Taq DNA polymerase (Qiagen) in 25 µl of the appropriate buffer.

    Article Title: Impairment of germline transmission after blastocyst injection with murine embryonic stem cells cultured with mouse hepatitis virus and mouse minute virus
    Article Snippet: Immediately thereafter, an RNase inhibitor (MBI Fermentas, St. Leon-Rot, Germany) was added to the RT mixture at a concentration of 10 U/μl. cDNA was synthesized using 12 μl RNA and the Omniscript Reverse Transcriptase kit (Qiagen). .. PCR was performed in a total volume of 20 μl using Taq DNA polymerase (Qiagen) for 35 cycles in a thermocycler (Biometra, Biomedizinische Analytik GmbH, Göttingen, Germany).

    TA Cloning:

    Article Title: Knockdown resistance in Anopheles vagus, An. sinensis, An. paraliae and An. peditaeniatus populations of the Mekong region
    Article Snippet: The resulting PCR product was cloned by use of the Original TA cloning kit according to the manufacturer's instructions (Invitrogen, Carlsbad, California). .. All AS-PCR assays were performed in a 50 μl reaction mixture containing: 1 × Qiagen PCR buffer, 1 × Q solution, 0.5 mM MgCl2 , 200 μM dNTP's, 400 nM of the outer forward and reverse primer, 500 nM of the inner resistant and sensitive primer (Figure ), 1 unit Taq DNA polymerase (Qiagen, Hilden, Germany) and 1 μl template.

    Construct:

    Article Title: Members of a Large Retroposon Family Are Determinants of Post-Transcriptional Gene Expression in Leishmania
    Article Snippet: The different LUC -chimeric constructs listed in were made as follows. .. The full-length 3′UTR of LmjF36.3810 and LmjF08.1270 transcripts from the termination codon to 434 bp beyond the poly(A) site in the case of LmjF36.3810, and to 84 bp beyond the poly(A) site in the case of LmjF08.1270, or the LmSIDER2 element or the 3′UTR lacking LmSIDER2, were amplified by PCR using Taq DNA polymerase (Qiagen, http://www.qiagen.com/ ) and primers with inserted BamHI or PstI restriction sites (see ).

    Article Title: First insights into the genetic diversity of Mycobacterium tuberculosis isolates from HIV-infected Mexican patients and mutations causing multidrug resistance
    Article Snippet: Bacteria were resuspended in 200 μl milli-Q water, boiled for 10 min, and cooled on ice or 5 min. Supernatant from bacterial lysates (2 μl) was added to MIRU-PCR mix (0.1 μl of HotStart Taq DNA polymerase (0.5 U) (Qiagen) with 4 μl of Q-solution, 0.5 mM each dATP, dCTP, dGTP, dTTP, 2 μl of PCR buffer, variable concentrations of each primer, and 1.5 mM MgCl2 ) in 20 μl final volume. .. Bacteria were resuspended in 200 μl milli-Q water, boiled for 10 min, and cooled on ice or 5 min. Supernatant from bacterial lysates (2 μl) was added to MIRU-PCR mix (0.1 μl of HotStart Taq DNA polymerase (0.5 U) (Qiagen) with 4 μl of Q-solution, 0.5 mM each dATP, dCTP, dGTP, dTTP, 2 μl of PCR buffer, variable concentrations of each primer, and 1.5 mM MgCl2 ) in 20 μl final volume.

    SYBR Green Assay:

    Article Title: Fractalkine (CX3CL1), GM-CSF and VEGF-a levels are reduced by statins in adult patients
    Article Snippet: Total RNA was reverse transcribed using SuperScript III Reverse Transcriptase with oligo-dT primers (Invitrogen). .. Real-time PCR analysis was performed using SYBR green and Taq DNA polymerase (Qiagen) on a Bio-Rad CFX96 Connect Real-Time PCR Detection System. .. PCR primers for CX3CL1, GM-CSF, ICAM, VCAM, CX3CR1, and VEGF-A were obtained from Qiagen.

    Incubation:

    Article Title: Fractalkine (CX3CL1), GM-CSF and VEGF-a levels are reduced by statins in adult patients
    Article Snippet: Where indicated, cells were incubated for 18 hours in human LDL cholesterol (100 μg/mL; Biomedical Technologies, Stoughton, MA, USA) and/or atorvastatin (100 nM; Sigma Scientific). .. Real-time PCR analysis was performed using SYBR green and Taq DNA polymerase (Qiagen) on a Bio-Rad CFX96 Connect Real-Time PCR Detection System.

    Stripping Membranes:

    Article Title: Quality not Quantity: The Role of Marine Natural Products in Drug Discovery and Reverse Chemical Proteomics
    Article Snippet: Taq DNA polymerase and QIAquick PCR purification kits were obtained from QIAGEN (USA). .. Taq DNA polymerase and QIAquick PCR purification kits were obtained from QIAGEN (USA).

    Expressing:

    Article Title: Fractalkine (CX3CL1), GM-CSF and VEGF-a levels are reduced by statins in adult patients
    Article Snippet: Real-time PCR analysis was performed using SYBR green and Taq DNA polymerase (Qiagen) on a Bio-Rad CFX96 Connect Real-Time PCR Detection System. .. PCR primers for CX3CL1, GM-CSF, ICAM, VCAM, CX3CR1, and VEGF-A were obtained from Qiagen.

    Article Title: Members of a Large Retroposon Family Are Determinants of Post-Transcriptional Gene Expression in Leishmania
    Article Snippet: The expression vector pSPYNEOαLUC was described previously [ ] and is referred to as LUC -control in the present study. .. The full-length 3′UTR of LmjF36.3810 and LmjF08.1270 transcripts from the termination codon to 434 bp beyond the poly(A) site in the case of LmjF36.3810, and to 84 bp beyond the poly(A) site in the case of LmjF08.1270, or the LmSIDER2 element or the 3′UTR lacking LmSIDER2, were amplified by PCR using Taq DNA polymerase (Qiagen, http://www.qiagen.com/ ) and primers with inserted BamHI or PstI restriction sites (see ).

    Article Title: The serine protease inhibitor serpinE2 is a novel target of ERK signaling involved in human colorectal tumorigenesis
    Article Snippet: Reverse transcription and PCR were performed using AMV-RT (Roche Diagnostics, Canada) and Taq DNA polymerase (Qiagen, Canada) according to the manufacturer's instructions. .. Real-time PCR analysis (QPCR) was performed using a Light-Cycler apparatus (Roche Diagnostic, Canada) as previously described [ ].

    Touchdown PCR:

    Article Title: Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Allelic Variants Relate to Shifts in Faecal Microbiota of Cystic Fibrosis Patients
    Article Snippet: PCR was performed with Taq DNA-polymerase (Hot Star Taq Plus, QIAGEN). .. PCR was performed with Taq DNA-polymerase (Hot Star Taq Plus, QIAGEN).

    Modification:

    Article Title: Silencing and Un-silencing of Tetracycline-Controlled Genes in Neurons
    Article Snippet: Since methylated cytosines have the same base-pairing characteristics as unmethylated cytosines, DNA is chemically modified to distinguish between the two species. .. PCRs were performed on MJ Research thermocyclers (Waltham, Massachusetts, United States) in a final volume of 25 µl containing 1× PCR Buffer, 1 U /Taq/ DNA polymerase (Qiagen), 200 µM dNTPs, 12.5 pmol each of forward and reverse primers, and 7 ng of bisulphite-treated genomic DNA.

    Article Title: Y-chromosome phylogeny in the evolutionary net of chamois (genus Rupicapra)
    Article Snippet: DNA from bones or teeth was extracted by a method modified from Catanneo et al. [ ] as described [ ]. .. Reactions were performed in a final volume of 20 μl containing 2 μl (≈ 40-70 ng) DNA, 0.5 mM of each primer, 1x PCR Buffer, 200 mM of each dNTP, 2.5 mM MgCl2 and 1 U of Taq DNA polymerase (Qiagen, Hilden, Germany).

    Transformation Assay:

    Article Title: Burkholderia cenocepacia conditional growth mutant library created by random promoter replacement of essential genes
    Article Snippet: Escherichia coli SY327 cells were transformed using the Z-competent buffer kit protocol (Zymo Research, Irvine, CA). .. DNA was amplified using a PTC-221 DNA engine (MJ Research, Waltham, MA) or an Eppendorf Mastercycler ep gradient S thermal cycler with either Taq DNA polymerase (Qiagen, Hilden, Germany) or Phusion High-Fidelity PCR Kit (New England Biolabs).

    Conjugation Assay:

    Article Title: Burkholderia cenocepacia conditional growth mutant library created by random promoter replacement of essential genes
    Article Snippet: Conjugation into B. cenocepacia K56-2 was accomplished by triparental mating (Craig et al. ) with E. coli DH5α carrying the helper plasmid pRK2013 (Figurski and Helinski ). .. DNA was amplified using a PTC-221 DNA engine (MJ Research, Waltham, MA) or an Eppendorf Mastercycler ep gradient S thermal cycler with either Taq DNA polymerase (Qiagen, Hilden, Germany) or Phusion High-Fidelity PCR Kit (New England Biolabs).

    Transfection:

    Article Title: Members of a Large Retroposon Family Are Determinants of Post-Transcriptional Gene Expression in Leishmania
    Article Snippet: Paragraph title: Plasmid construction and transfections. ... The full-length 3′UTR of LmjF36.3810 and LmjF08.1270 transcripts from the termination codon to 434 bp beyond the poly(A) site in the case of LmjF36.3810, and to 84 bp beyond the poly(A) site in the case of LmjF08.1270, or the LmSIDER2 element or the 3′UTR lacking LmSIDER2, were amplified by PCR using Taq DNA polymerase (Qiagen, http://www.qiagen.com/ ) and primers with inserted BamHI or PstI restriction sites (see ).

    Methylation:

    Article Title: Silencing and Un-silencing of Tetracycline-Controlled Genes in Neurons
    Article Snippet: Since methylated cytosines have the same base-pairing characteristics as unmethylated cytosines, DNA is chemically modified to distinguish between the two species. .. PCRs were performed on MJ Research thermocyclers (Waltham, Massachusetts, United States) in a final volume of 25 µl containing 1× PCR Buffer, 1 U /Taq/ DNA polymerase (Qiagen), 200 µM dNTPs, 12.5 pmol each of forward and reverse primers, and 7 ng of bisulphite-treated genomic DNA.

    Cell Culture:

    Article Title: The serine protease inhibitor serpinE2 is a novel target of ERK signaling involved in human colorectal tumorigenesis
    Article Snippet: Total RNA was extracted from cultured cell lines or human colorectal adenoma or tumors and their respective adjacent healthy mucosa using the RNeasy mini kit (Qiagen, Canada) using gDNA Eliminator spin columns or an on-column DNAse I digestion step (human samples). .. Reverse transcription and PCR were performed using AMV-RT (Roche Diagnostics, Canada) and Taq DNA polymerase (Qiagen, Canada) according to the manufacturer's instructions.

    Polymerase Chain Reaction:

    Article Title: Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Allelic Variants Relate to Shifts in Faecal Microbiota of Cystic Fibrosis Patients
    Article Snippet: Universal primers GCclamp-U968 ( 5′-CGC CCG GGG CGC GCC CCG GGC GGG GCG GGG GCA CGG GGG GAA CGC GAA GAA CCT TAC-3′ ) and L1401 (5′-GCG TGT GTA CAA GAC CC-3′) were used to amplify the V6–V8 region of bacterial gene coding for 16S rRNA . .. PCR was performed with Taq DNA-polymerase (Hot Star Taq Plus, QIAGEN). .. PCR reaction (25 µL) contained 1× buffer per PCR, 2.5 mM MgCl2 , 200 µM for each dNTP, 0.5 µM of GCclamp-U968 and L1401 primers, 1.25 U of Taq polymerase and 100 ng of total DNA.

    Article Title: Burkholderia cenocepacia conditional growth mutant library created by random promoter replacement of essential genes
    Article Snippet: Conjugation into B. cenocepacia K56-2 was accomplished by triparental mating (Craig et al. ) with E. coli DH5α carrying the helper plasmid pRK2013 (Figurski and Helinski ). .. DNA was amplified using a PTC-221 DNA engine (MJ Research, Waltham, MA) or an Eppendorf Mastercycler ep gradient S thermal cycler with either Taq DNA polymerase (Qiagen, Hilden, Germany) or Phusion High-Fidelity PCR Kit (New England Biolabs). .. Amplification conditions were optimized for each primer pair.

    Article Title: Ancestral Gene Organization in the Mitochondrial Genome of Thyridosmylus langii (McLachlan, 1870) (Neuroptera: Osmylidae) and Implications for Lacewing Evolution
    Article Snippet: Paragraph title: PCR amplification, cloning and sequencing ... Short PCRs were conducted using Qiagen Taq DNA polymerase (Qiagen, Beijing, China) with the following cycling conditions: 5 min at 94°C, followed by 35 cycles of 50 s at 94°C, 30 s at 40–55°C, and 1–2 min at 72°C.

    Article Title: Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans
    Article Snippet: Extracted genomic DNA was quantified using a NanoDrop ND1000 spectrophotometer (Peqlab; Erlangen, Germany). .. PCR amplification of genomic DNA was carried out using the HotMasterMix 2.5x from 5 PRIME (Hamburg, Germany) according to the manufacturer’s protocol or the Taq DNA polymerase from Qiagen (Hilden, Germany) in reaction buffer containing 200 μM (each) deoxynucleotide triphosphates (dNTPs), 1 μM (each) oligonucleotide primers and ca. .. 10 – 25 ng of genomic DNA in a final volume of 20 μl.

    Article Title: Identification of Key Uric Acid Synthesis Pathway in a Unique Mutant Silkworm Bombyx mori Model of Parkinson's Disease
    Article Snippet: Protein concentrations were determined using a Bradford assay kit (Pierce, Rockford, IL, USA), according to the manufacturer’s instructions. .. Total RNA from 500 brains of day 3–5 fifth instar larvae were treated with DNase and processed for cDNA synthesis using primers of 12 to 18 oligo(dT) and SuperScript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA). cDNA was amplified by PCR using Taq DNA polymerase (Qiagen) with the primers for tyrosine hydroxylase (TH; 5′-ACACAACAGTGGTTCAGTCG-3′ and 5′-AGGAGTCTCGCAATGTACAC-3′ ), actin ( 5′-TATCGCCGACAGGATGCAGAAGGA-3′ and 5′-TAGAAGCACTTGCGGTGAACGATG-3′ ) and DJ-1 ( 5′-CATTTGTGCTGCTTCCATAGCGTT-3′ and 5′-CATTCCCTTTTCGACTTGATCGGC-3′ ), as described previously . .. RT-PCR for B. mori actin was used as a positive loading control.

    Article Title: Quality not Quantity: The Role of Marine Natural Products in Drug Discovery and Reverse Chemical Proteomics
    Article Snippet: Oligonucleotides (primers) were obtained from Sigma-Genosys (Australia). .. Taq DNA polymerase and QIAquick PCR purification kits were obtained from QIAGEN (USA). .. Hin fI restriction endonuclease and NEB buffer 2 were obtained from New England Biolabs Inc. (USA).

    Article Title: Identification of More Than Two Paternal Haplotypes of the Ovine Fatty Acid-Binding Protein 4 (FABP4) Gene in Half-Sib Families: Evidence of Intragenic Meiotic Recombination
    Article Snippet: Briefly, region 1 (exon 2 - intron 2) was amplified using the PCR primers 5′-tgtgggctttgctaccag-3′ and 5′-taaatgggagacaattcacc-3′, while region 2 (exon 3 - intron 3) was amplified using the primers 5′-acttagatgaaggtgctctg-3′ and 5′- ctcaggactaaacaactcatg-3′. .. Amplifications were performed in a 15-µL reaction containing the DNA on one 1.2 mm punch of FTA card, 0.25 µM of each paired primer set, 150 µM dNTPs (Bioline, London, UK), 2.5 mM Mg2+ , 0.5 U Taq DNA polymerase (Qiagen, Hilden, Germany) and 1× the reaction buffer supplied with the enzyme.

    Article Title: The invasive New Guinea flatworm Platydemus manokwari in France, the first record for Europe: time for action is now
    Article Snippet: A fragment of 424 bp of COI gene was amplified with the primers COI-ASmit1 (forward 5′-TTTTTTGGGCATCCTGAGGTTTAT-3′) and COI-ASmit2 (reverse 5′-TAAAGAAAGAACATAATGAAAATG-3′) ( ). .. The PCR reaction was performed in 20 µl, containing 1 ng of DNA, 1× CoralLoad PCR buffer, 3 Mm MgCl2, 66 µM of each dNTP, 0.15 µM of each primer, and 0.5 units of Taq DNA polymerase (Qiagen). .. The amplification protocol was: 4′ at 94°C, followed by 40 cycles of 94°C for 30″, 48°C for 40″, 72°C for 50″, with a final extension at 72°C for 7′.

    Article Title: Silencing and Un-silencing of Tetracycline-Controlled Genes in Neurons
    Article Snippet: In a subsequent PCR uracil is replicated as thymine. .. PCRs were performed on MJ Research thermocyclers (Waltham, Massachusetts, United States) in a final volume of 25 µl containing 1× PCR Buffer, 1 U /Taq/ DNA polymerase (Qiagen), 200 µM dNTPs, 12.5 pmol each of forward and reverse primers, and 7 ng of bisulphite-treated genomic DNA. .. The amplification conditions were 95°C for 15 min and 40 cycles of 95°C for 1 min, 55°C for 45 sec and 72°C for 1 min and a final extension step of 10 min at 72°C.

    Article Title: Faithful transcription initiation from a mitochondrial promoter in transgenic plastids
    Article Snippet: 5′ termini of in vivo synthesized transcripts were determined employing a 5′-RACE technique combined with tobacco acid pyrophosphatase (TAP) treatment as described by Kühn et al. ( ) with the following modifications: The gene-specific primer used for reverse transcription was Pa (5′-CTGTTGTGCCCAGTCATAG-3′). .. The products of reverse transcription (RT) were amplified in a first PCR step by using 4 µl of the RT reaction, 5 pmol of both the adapter-specific forward primer P1a (5′-CGAATTCCTGTAGAACGAACACTAGAAG-3′) and the gene-specific reverse primer Pb (5′-ATAGTTTGTGGAGAATTTCGCTTCC-3′), 200 µM of each dNTP and 0.5 U of Taq DNA polymerase (Qiagen) in 25 µl of the appropriate buffer. .. Amplification conditions comprised an initial denaturation step at 94°C for 1 min followed by 35 cycles of 95°C for 20 s, 58°C for 20 s and 72°C for 2 min and a final extension step at 72°C for 10 min. A 1 µl aliquot of the first PCR reaction was used as template for subsequent nested PCRs set up essentially as the first PCR in a volume of 50 µl with 10 pmol of both the gene-specific primer Pc (5′-CTGTGTTAAGCATAGGGCCTAACTAGC′3′) and the adapter-specific primer P1a.

    Article Title: Computational and Experimental Analysis of Redundancy in the Central Metabolism of Geobacter sulfurreducens
    Article Snippet: Genomic DNA was purified using the MasterPure Complete DNA & RNA purification kit (Epicentre Technologies) PCR product purification and gel extraction were carried out using the PCR purification kit and the Qiaquick gel extraction kit (Qiagen). .. All PCR amplifications were done using Taq DNA polymerase (Qiagen). .. Cell-free extracts were prepared from 100 ml mid-log cultures.

    Article Title: Members of a Large Retroposon Family Are Determinants of Post-Transcriptional Gene Expression in Leishmania
    Article Snippet: The different LUC -chimeric constructs listed in were made as follows. .. The full-length 3′UTR of LmjF36.3810 and LmjF08.1270 transcripts from the termination codon to 434 bp beyond the poly(A) site in the case of LmjF36.3810, and to 84 bp beyond the poly(A) site in the case of LmjF08.1270, or the LmSIDER2 element or the 3′UTR lacking LmSIDER2, were amplified by PCR using Taq DNA polymerase (Qiagen, http://www.qiagen.com/ ) and primers with inserted BamHI or PstI restriction sites (see ). .. PCR products were cloned into vector pCR2.1 (Invitrogen, http://www.invitrogen.com/ ), digested with BamHI or PstI (New England Biolabs, http://www.neb.com/ ) and subcloned into the BamHI site downstream of the LUC gene in vector pSPYNEOαLUC [ ].

    Article Title: Impairment of germline transmission after blastocyst injection with murine embryonic stem cells cultured with mouse hepatitis virus and mouse minute virus
    Article Snippet: A double-distilled water sample was used as a negative control in both the RT and the PCR steps. .. PCR was performed in a total volume of 20 μl using Taq DNA polymerase (Qiagen) for 35 cycles in a thermocycler (Biometra, Biomedizinische Analytik GmbH, Göttingen, Germany). .. The last cycle was followed by a 10-min extension period at 72°C.

    Article Title: The serine protease inhibitor serpinE2 is a novel target of ERK signaling involved in human colorectal tumorigenesis
    Article Snippet: Total RNA was extracted from cultured cell lines or human colorectal adenoma or tumors and their respective adjacent healthy mucosa using the RNeasy mini kit (Qiagen, Canada) using gDNA Eliminator spin columns or an on-column DNAse I digestion step (human samples). .. Reverse transcription and PCR were performed using AMV-RT (Roche Diagnostics, Canada) and Taq DNA polymerase (Qiagen, Canada) according to the manufacturer's instructions. .. Real-time PCR analysis (QPCR) was performed using a Light-Cycler apparatus (Roche Diagnostic, Canada) as previously described [ ].

    Article Title: A specific insertion of a solo-LTR characterizes the Y-chromosome of Bryonia dioica (Cucurbitaceae)
    Article Snippet: Cycle conditions for the second PCR: 5 cycles of 25 s at 94°C, 3 min at 72°C, 20 cycles of 25 s at 94°C, 3 min at 67°C for amplification and 67°C for 10 min after the final cycle. .. All PCR mixtures contained 1.25 Units Taq DNA polymerase (QIAGEN GmbH, Hilden, Germany [Qiagen]), 1× Taq buffer, 15 mM MgCl2 , 10 mM of each dNTP and 10 pmol of each primer in a total volume of 50 μl. .. Gene-specific primers (GSP) are listed in Table .

    Article Title: Knockdown resistance in Anopheles vagus, An. sinensis, An. paraliae and An. peditaeniatus populations of the Mekong region
    Article Snippet: The AS-PCR assays were optimized by running genomic DNA templates that had been previously genotyped by DNA sequencing. .. All AS-PCR assays were performed in a 50 μl reaction mixture containing: 1 × Qiagen PCR buffer, 1 × Q solution, 0.5 mM MgCl2 , 200 μM dNTP's, 400 nM of the outer forward and reverse primer, 500 nM of the inner resistant and sensitive primer (Figure ), 1 unit Taq DNA polymerase (Qiagen, Hilden, Germany) and 1 μl template. .. The cycling conditions described above for the amplification of the DIIS6 region were used.

    Article Title: Y-chromosome phylogeny in the evolutionary net of chamois (genus Rupicapra)
    Article Snippet: SE47/SE48 primers amplify one DNA fragment for females (indicating the presence of X-chromosome) and two for males (indicating the presence of a X and a Y chromosome). .. Reactions were performed in a final volume of 20 μl containing 2 μl (≈ 40-70 ng) DNA, 0.5 mM of each primer, 1x PCR Buffer, 200 mM of each dNTP, 2.5 mM MgCl2 and 1 U of Taq DNA polymerase (Qiagen, Hilden, Germany). .. Amplification was carried out in PE GeneAmp PCR 9700 thermal cycler (Applied Biosystems, Foster City, CA) with an initial step of 5 min at 94°C, 35 cycles of 30 s at 94°C, 30 s at 60°C and 1 min at 72°C, followed by 5 min at 72°C.

    Article Title: Autosomal dominant hereditary spastic paraplegia: Novel mutations in the REEP1 gene (SPG31)
    Article Snippet: Screening of the entire coding region of the REEP1 gene (seven exons including the flanking intronic sequence, at least 40 bp from each end) was performed using polymerase chain reaction (PCR) with consecutive DHPLC analysis. .. PCR reactions for exon 1 were performed in a total volume of 10 μl, containing 60 ng DNA, 10 mmol of each dNTP DEAZA (Roche, Mannheim, Germany), 3 mmol MgCl2 (Qiagen, Hilden, Germany) 10 pmol of each primer, 0.25 U Taq polymerase Hotstar Taq Plus (Qiagen, Hilden, Germany), DMSO 5% and 10 × buffer (contains 15 mM MgCl2 ); (Qiagen, Hilden, Germany). .. PCR reactions for exons 2 to 7 were performed in a total volume of 12.5 μl, containing 100 ng DNA, 2.5 mmol of each dNTP, 2 mmol MgCl2 , 10 pmol of each primer, 0.5 U Taq polymerase (Genecraft, Lüdinghausen, Germany) and 10 × buffer BioTherm without MgCl2 (Genecraft, Lüdinghausen, Germany).

    Article Title: First insights into the genetic diversity of Mycobacterium tuberculosis isolates from HIV-infected Mexican patients and mutations causing multidrug resistance
    Article Snippet: MIRU-VNTR typing was performed as described previously [ ]. .. Bacteria were resuspended in 200 μl milli-Q water, boiled for 10 min, and cooled on ice or 5 min. Supernatant from bacterial lysates (2 μl) was added to MIRU-PCR mix (0.1 μl of HotStart Taq DNA polymerase (0.5 U) (Qiagen) with 4 μl of Q-solution, 0.5 mM each dATP, dCTP, dGTP, dTTP, 2 μl of PCR buffer, variable concentrations of each primer, and 1.5 mM MgCl2 ) in 20 μl final volume. .. The oligonucleotides used corresponded to the flanking regions of the 12 polymorphic MIRU-VNTR loci identified in the M. tuberculosis H37Rv genome as described by Supply et al [ ].

    DNA Sequencing:

    Article Title: Burkholderia cenocepacia conditional growth mutant library created by random promoter replacement of essential genes
    Article Snippet: DNA was amplified using a PTC-221 DNA engine (MJ Research, Waltham, MA) or an Eppendorf Mastercycler ep gradient S thermal cycler with either Taq DNA polymerase (Qiagen, Hilden, Germany) or Phusion High-Fidelity PCR Kit (New England Biolabs). .. PCR products and plasmids were purified using QIAquick PCR Purification Kit (Qiagen) and QIAprep Spin Miniprep Kit (Qiagen), respectively.

    Article Title: Knockdown resistance in Anopheles vagus, An. sinensis, An. paraliae and An. peditaeniatus populations of the Mekong region
    Article Snippet: The AS-PCR assays were optimized by running genomic DNA templates that had been previously genotyped by DNA sequencing. .. All AS-PCR assays were performed in a 50 μl reaction mixture containing: 1 × Qiagen PCR buffer, 1 × Q solution, 0.5 mM MgCl2 , 200 μM dNTP's, 400 nM of the outer forward and reverse primer, 500 nM of the inner resistant and sensitive primer (Figure ), 1 unit Taq DNA polymerase (Qiagen, Hilden, Germany) and 1 μl template.

    Sequencing:

    Article Title: Ancestral Gene Organization in the Mitochondrial Genome of Thyridosmylus langii (McLachlan, 1870) (Neuroptera: Osmylidae) and Implications for Lacewing Evolution
    Article Snippet: Paragraph title: PCR amplification, cloning and sequencing ... Short PCRs were conducted using Qiagen Taq DNA polymerase (Qiagen, Beijing, China) with the following cycling conditions: 5 min at 94°C, followed by 35 cycles of 50 s at 94°C, 30 s at 40–55°C, and 1–2 min at 72°C.

    Article Title: Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans
    Article Snippet: PCR amplification of genomic DNA was carried out using the HotMasterMix 2.5x from 5 PRIME (Hamburg, Germany) according to the manufacturer’s protocol or the Taq DNA polymerase from Qiagen (Hilden, Germany) in reaction buffer containing 200 μM (each) deoxynucleotide triphosphates (dNTPs), 1 μM (each) oligonucleotide primers and ca. .. PCR products were purified using the HiYield Gel/PCR clean-up and Gel-Extraction Kit (SLG; Gauting, Germany) according to the manufacturer’s protocol and visualized by gel electrophoresis (1% agarose).

    Article Title: Identification of Key Uric Acid Synthesis Pathway in a Unique Mutant Silkworm Bombyx mori Model of Parkinson's Disease
    Article Snippet: Total RNA from 500 brains of day 3–5 fifth instar larvae were treated with DNase and processed for cDNA synthesis using primers of 12 to 18 oligo(dT) and SuperScript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA). cDNA was amplified by PCR using Taq DNA polymerase (Qiagen) with the primers for tyrosine hydroxylase (TH; 5′-ACACAACAGTGGTTCAGTCG-3′ and 5′-AGGAGTCTCGCAATGTACAC-3′ ), actin ( 5′-TATCGCCGACAGGATGCAGAAGGA-3′ and 5′-TAGAAGCACTTGCGGTGAACGATG-3′ ) and DJ-1 ( 5′-CATTTGTGCTGCTTCCATAGCGTT-3′ and 5′-CATTCCCTTTTCGACTTGATCGGC-3′ ), as described previously . .. Amplified PCR products were separated by agarose gel electrophoresis, stained with ethidium bromide, and visualized under UV light.

    Article Title: Silencing and Un-silencing of Tetracycline-Controlled Genes in Neurons
    Article Snippet: Paragraph title: Sodium bisulphite conversion, PCR amplification and sequencing ... PCRs were performed on MJ Research thermocyclers (Waltham, Massachusetts, United States) in a final volume of 25 µl containing 1× PCR Buffer, 1 U /Taq/ DNA polymerase (Qiagen), 200 µM dNTPs, 12.5 pmol each of forward and reverse primers, and 7 ng of bisulphite-treated genomic DNA.

    Article Title: Faithful transcription initiation from a mitochondrial promoter in transgenic plastids
    Article Snippet: The products of reverse transcription (RT) were amplified in a first PCR step by using 4 µl of the RT reaction, 5 pmol of both the adapter-specific forward primer P1a (5′-CGAATTCCTGTAGAACGAACACTAGAAG-3′) and the gene-specific reverse primer Pb (5′-ATAGTTTGTGGAGAATTTCGCTTCC-3′), 200 µM of each dNTP and 0.5 U of Taq DNA polymerase (Qiagen) in 25 µl of the appropriate buffer. .. Amplification conditions comprised an initial denaturation step at 94°C for 1 min followed by 35 cycles of 95°C for 20 s, 58°C for 20 s and 72°C for 2 min and a final extension step at 72°C for 10 min. A 1 µl aliquot of the first PCR reaction was used as template for subsequent nested PCRs set up essentially as the first PCR in a volume of 50 µl with 10 pmol of both the gene-specific primer Pc (5′-CTGTGTTAAGCATAGGGCCTAACTAGC′3′) and the adapter-specific primer P1a.

    Article Title: A specific insertion of a solo-LTR characterizes the Y-chromosome of Bryonia dioica (Cucurbitaceae)
    Article Snippet: Paragraph title: Sequencing the 5' and 3' regions flanking BdY1 ... All PCR mixtures contained 1.25 Units Taq DNA polymerase (QIAGEN GmbH, Hilden, Germany [Qiagen]), 1× Taq buffer, 15 mM MgCl2 , 10 mM of each dNTP and 10 pmol of each primer in a total volume of 50 μl.

    Article Title: Knockdown resistance in Anopheles vagus, An. sinensis, An. paraliae and An. peditaeniatus populations of the Mekong region
    Article Snippet: Plasmid and direct PCR sequencing were done by the VIB genetic service facility (University of Antwerp, Belgium) and aligned with ClustalW version 1.3 [ ]. .. All AS-PCR assays were performed in a 50 μl reaction mixture containing: 1 × Qiagen PCR buffer, 1 × Q solution, 0.5 mM MgCl2 , 200 μM dNTP's, 400 nM of the outer forward and reverse primer, 500 nM of the inner resistant and sensitive primer (Figure ), 1 unit Taq DNA polymerase (Qiagen, Hilden, Germany) and 1 μl template.

    Article Title: Autosomal dominant hereditary spastic paraplegia: Novel mutations in the REEP1 gene (SPG31)
    Article Snippet: Screening of the entire coding region of the REEP1 gene (seven exons including the flanking intronic sequence, at least 40 bp from each end) was performed using polymerase chain reaction (PCR) with consecutive DHPLC analysis. .. PCR reactions for exon 1 were performed in a total volume of 10 μl, containing 60 ng DNA, 10 mmol of each dNTP DEAZA (Roche, Mannheim, Germany), 3 mmol MgCl2 (Qiagen, Hilden, Germany) 10 pmol of each primer, 0.25 U Taq polymerase Hotstar Taq Plus (Qiagen, Hilden, Germany), DMSO 5% and 10 × buffer (contains 15 mM MgCl2 ); (Qiagen, Hilden, Germany).

    Cellular Antioxidant Activity Assay:

    Article Title: Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Allelic Variants Relate to Shifts in Faecal Microbiota of Cystic Fibrosis Patients
    Article Snippet: Universal primers GCclamp-U968 ( 5′-CGC CCG GGG CGC GCC CCG GGC GGG GCG GGG GCA CGG GGG GAA CGC GAA GAA CCT TAC-3′ ) and L1401 (5′-GCG TGT GTA CAA GAC CC-3′) were used to amplify the V6–V8 region of bacterial gene coding for 16S rRNA . .. PCR was performed with Taq DNA-polymerase (Hot Star Taq Plus, QIAGEN).

    DNA Extraction:

    Article Title: Y-chromosome phylogeny in the evolutionary net of chamois (genus Rupicapra)
    Article Snippet: Paragraph title: Sampling, DNA extraction and sex determination ... Reactions were performed in a final volume of 20 μl containing 2 μl (≈ 40-70 ng) DNA, 0.5 mM of each primer, 1x PCR Buffer, 200 mM of each dNTP, 2.5 mM MgCl2 and 1 U of Taq DNA polymerase (Qiagen, Hilden, Germany).

    Nucleic Acid Electrophoresis:

    Article Title: Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans
    Article Snippet: PCR amplification of genomic DNA was carried out using the HotMasterMix 2.5x from 5 PRIME (Hamburg, Germany) according to the manufacturer’s protocol or the Taq DNA polymerase from Qiagen (Hilden, Germany) in reaction buffer containing 200 μM (each) deoxynucleotide triphosphates (dNTPs), 1 μM (each) oligonucleotide primers and ca. .. PCR amplification of genomic DNA was carried out using the HotMasterMix 2.5x from 5 PRIME (Hamburg, Germany) according to the manufacturer’s protocol or the Taq DNA polymerase from Qiagen (Hilden, Germany) in reaction buffer containing 200 μM (each) deoxynucleotide triphosphates (dNTPs), 1 μM (each) oligonucleotide primers and ca.

    In Vivo:

    Article Title: Faithful transcription initiation from a mitochondrial promoter in transgenic plastids
    Article Snippet: Paragraph title: 5′-end mapping of in vivo and in vitro -synthesized RNAs ... The products of reverse transcription (RT) were amplified in a first PCR step by using 4 µl of the RT reaction, 5 pmol of both the adapter-specific forward primer P1a (5′-CGAATTCCTGTAGAACGAACACTAGAAG-3′) and the gene-specific reverse primer Pb (5′-ATAGTTTGTGGAGAATTTCGCTTCC-3′), 200 µM of each dNTP and 0.5 U of Taq DNA polymerase (Qiagen) in 25 µl of the appropriate buffer.

    Real-time Polymerase Chain Reaction:

    Article Title: Fractalkine (CX3CL1), GM-CSF and VEGF-a levels are reduced by statins in adult patients
    Article Snippet: Total RNA was reverse transcribed using SuperScript III Reverse Transcriptase with oligo-dT primers (Invitrogen). .. Real-time PCR analysis was performed using SYBR green and Taq DNA polymerase (Qiagen) on a Bio-Rad CFX96 Connect Real-Time PCR Detection System. .. PCR primers for CX3CL1, GM-CSF, ICAM, VCAM, CX3CR1, and VEGF-A were obtained from Qiagen.

    Electroporation:

    Article Title: Members of a Large Retroposon Family Are Determinants of Post-Transcriptional Gene Expression in Leishmania
    Article Snippet: The full-length 3′UTR of LmjF36.3810 and LmjF08.1270 transcripts from the termination codon to 434 bp beyond the poly(A) site in the case of LmjF36.3810, and to 84 bp beyond the poly(A) site in the case of LmjF08.1270, or the LmSIDER2 element or the 3′UTR lacking LmSIDER2, were amplified by PCR using Taq DNA polymerase (Qiagen, http://www.qiagen.com/ ) and primers with inserted BamHI or PstI restriction sites (see ). .. PCR products were cloned into vector pCR2.1 (Invitrogen, http://www.invitrogen.com/ ), digested with BamHI or PstI (New England Biolabs, http://www.neb.com/ ) and subcloned into the BamHI site downstream of the LUC gene in vector pSPYNEOαLUC [ ].

    Isolation:

    Article Title: Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans
    Article Snippet: For the isolation of genomic DNA from strain Ivo14T and further reference strains the MasterPure™ Gram Positive DNA Purification Kit from Epicentre (Madison, USA) was used according to the instructions provided by the manufacturer. .. PCR amplification of genomic DNA was carried out using the HotMasterMix 2.5x from 5 PRIME (Hamburg, Germany) according to the manufacturer’s protocol or the Taq DNA polymerase from Qiagen (Hilden, Germany) in reaction buffer containing 200 μM (each) deoxynucleotide triphosphates (dNTPs), 1 μM (each) oligonucleotide primers and ca.

    Purification:

    Article Title: Burkholderia cenocepacia conditional growth mutant library created by random promoter replacement of essential genes
    Article Snippet: DNA was amplified using a PTC-221 DNA engine (MJ Research, Waltham, MA) or an Eppendorf Mastercycler ep gradient S thermal cycler with either Taq DNA polymerase (Qiagen, Hilden, Germany) or Phusion High-Fidelity PCR Kit (New England Biolabs). .. DNA was amplified using a PTC-221 DNA engine (MJ Research, Waltham, MA) or an Eppendorf Mastercycler ep gradient S thermal cycler with either Taq DNA polymerase (Qiagen, Hilden, Germany) or Phusion High-Fidelity PCR Kit (New England Biolabs).

    Article Title: Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans
    Article Snippet: PCR amplification of genomic DNA was carried out using the HotMasterMix 2.5x from 5 PRIME (Hamburg, Germany) according to the manufacturer’s protocol or the Taq DNA polymerase from Qiagen (Hilden, Germany) in reaction buffer containing 200 μM (each) deoxynucleotide triphosphates (dNTPs), 1 μM (each) oligonucleotide primers and ca. .. PCR amplification of genomic DNA was carried out using the HotMasterMix 2.5x from 5 PRIME (Hamburg, Germany) according to the manufacturer’s protocol or the Taq DNA polymerase from Qiagen (Hilden, Germany) in reaction buffer containing 200 μM (each) deoxynucleotide triphosphates (dNTPs), 1 μM (each) oligonucleotide primers and ca.

    Article Title: Quality not Quantity: The Role of Marine Natural Products in Drug Discovery and Reverse Chemical Proteomics
    Article Snippet: Oligonucleotides (primers) were obtained from Sigma-Genosys (Australia). .. Taq DNA polymerase and QIAquick PCR purification kits were obtained from QIAGEN (USA). .. Hin fI restriction endonuclease and NEB buffer 2 were obtained from New England Biolabs Inc. (USA).

    Article Title: The invasive New Guinea flatworm Platydemus manokwari in France, the first record for Europe: time for action is now
    Article Snippet: The PCR reaction was performed in 20 µl, containing 1 ng of DNA, 1× CoralLoad PCR buffer, 3 Mm MgCl2, 66 µM of each dNTP, 0.15 µM of each primer, and 0.5 units of Taq DNA polymerase (Qiagen). .. The amplification protocol was: 4′ at 94°C, followed by 40 cycles of 94°C for 30″, 48°C for 40″, 72°C for 50″, with a final extension at 72°C for 7′.

    Article Title: Silencing and Un-silencing of Tetracycline-Controlled Genes in Neurons
    Article Snippet: Purified genomic DNA was treated with sodium bisulphite, resulting in the conversion of unmethylated cytosine to uracil. .. PCRs were performed on MJ Research thermocyclers (Waltham, Massachusetts, United States) in a final volume of 25 µl containing 1× PCR Buffer, 1 U /Taq/ DNA polymerase (Qiagen), 200 µM dNTPs, 12.5 pmol each of forward and reverse primers, and 7 ng of bisulphite-treated genomic DNA.

    Article Title: Computational and Experimental Analysis of Redundancy in the Central Metabolism of Geobacter sulfurreducens
    Article Snippet: Genomic DNA was purified using the MasterPure Complete DNA & RNA purification kit (Epicentre Technologies) PCR product purification and gel extraction were carried out using the PCR purification kit and the Qiaquick gel extraction kit (Qiagen). .. All PCR amplifications were done using Taq DNA polymerase (Qiagen).

    Article Title: Members of a Large Retroposon Family Are Determinants of Post-Transcriptional Gene Expression in Leishmania
    Article Snippet: The full-length 3′UTR of LmjF36.3810 and LmjF08.1270 transcripts from the termination codon to 434 bp beyond the poly(A) site in the case of LmjF36.3810, and to 84 bp beyond the poly(A) site in the case of LmjF08.1270, or the LmSIDER2 element or the 3′UTR lacking LmSIDER2, were amplified by PCR using Taq DNA polymerase (Qiagen, http://www.qiagen.com/ ) and primers with inserted BamHI or PstI restriction sites (see ). .. PCR products were cloned into vector pCR2.1 (Invitrogen, http://www.invitrogen.com/ ), digested with BamHI or PstI (New England Biolabs, http://www.neb.com/ ) and subcloned into the BamHI site downstream of the LUC gene in vector pSPYNEOαLUC [ ].

    Article Title: A specific insertion of a solo-LTR characterizes the Y-chromosome of Bryonia dioica (Cucurbitaceae)
    Article Snippet: All PCR mixtures contained 1.25 Units Taq DNA polymerase (QIAGEN GmbH, Hilden, Germany [Qiagen]), 1× Taq buffer, 15 mM MgCl2 , 10 mM of each dNTP and 10 pmol of each primer in a total volume of 50 μl. .. All PCR mixtures contained 1.25 Units Taq DNA polymerase (QIAGEN GmbH, Hilden, Germany [Qiagen]), 1× Taq buffer, 15 mM MgCl2 , 10 mM of each dNTP and 10 pmol of each primer in a total volume of 50 μl.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Identification of Key Uric Acid Synthesis Pathway in a Unique Mutant Silkworm Bombyx mori Model of Parkinson's Disease
    Article Snippet: Paragraph title: RT-PCR ... Total RNA from 500 brains of day 3–5 fifth instar larvae were treated with DNase and processed for cDNA synthesis using primers of 12 to 18 oligo(dT) and SuperScript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA). cDNA was amplified by PCR using Taq DNA polymerase (Qiagen) with the primers for tyrosine hydroxylase (TH; 5′-ACACAACAGTGGTTCAGTCG-3′ and 5′-AGGAGTCTCGCAATGTACAC-3′ ), actin ( 5′-TATCGCCGACAGGATGCAGAAGGA-3′ and 5′-TAGAAGCACTTGCGGTGAACGATG-3′ ) and DJ-1 ( 5′-CATTTGTGCTGCTTCCATAGCGTT-3′ and 5′-CATTCCCTTTTCGACTTGATCGGC-3′ ), as described previously .

    Article Title: Fractalkine (CX3CL1), GM-CSF and VEGF-a levels are reduced by statins in adult patients
    Article Snippet: Real-time PCR analysis was performed using SYBR green and Taq DNA polymerase (Qiagen) on a Bio-Rad CFX96 Connect Real-Time PCR Detection System. .. PCR primers for CX3CL1, GM-CSF, ICAM, VCAM, CX3CR1, and VEGF-A were obtained from Qiagen.

    Gel Extraction:

    Article Title: Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans
    Article Snippet: PCR amplification of genomic DNA was carried out using the HotMasterMix 2.5x from 5 PRIME (Hamburg, Germany) according to the manufacturer’s protocol or the Taq DNA polymerase from Qiagen (Hilden, Germany) in reaction buffer containing 200 μM (each) deoxynucleotide triphosphates (dNTPs), 1 μM (each) oligonucleotide primers and ca. .. PCR amplification of genomic DNA was carried out using the HotMasterMix 2.5x from 5 PRIME (Hamburg, Germany) according to the manufacturer’s protocol or the Taq DNA polymerase from Qiagen (Hilden, Germany) in reaction buffer containing 200 μM (each) deoxynucleotide triphosphates (dNTPs), 1 μM (each) oligonucleotide primers and ca.

    Article Title: Computational and Experimental Analysis of Redundancy in the Central Metabolism of Geobacter sulfurreducens
    Article Snippet: Genomic DNA was purified using the MasterPure Complete DNA & RNA purification kit (Epicentre Technologies) PCR product purification and gel extraction were carried out using the PCR purification kit and the Qiaquick gel extraction kit (Qiagen). .. All PCR amplifications were done using Taq DNA polymerase (Qiagen).

    cDNA Library Assay:

    Article Title: Quality not Quantity: The Role of Marine Natural Products in Drug Discovery and Reverse Chemical Proteomics
    Article Snippet: T7Select1-1b human brain cDNA library was a kind gift from Prof. David Austin (Department of Chemistry, Yale University). .. Taq DNA polymerase and QIAquick PCR purification kits were obtained from QIAGEN (USA).

    Plasmid Preparation:

    Article Title: Burkholderia cenocepacia conditional growth mutant library created by random promoter replacement of essential genes
    Article Snippet: Conjugation into B. cenocepacia K56-2 was accomplished by triparental mating (Craig et al. ) with E. coli DH5α carrying the helper plasmid pRK2013 (Figurski and Helinski ). .. DNA was amplified using a PTC-221 DNA engine (MJ Research, Waltham, MA) or an Eppendorf Mastercycler ep gradient S thermal cycler with either Taq DNA polymerase (Qiagen, Hilden, Germany) or Phusion High-Fidelity PCR Kit (New England Biolabs).

    Article Title: Ancestral Gene Organization in the Mitochondrial Genome of Thyridosmylus langii (McLachlan, 1870) (Neuroptera: Osmylidae) and Implications for Lacewing Evolution
    Article Snippet: Short PCRs were conducted using Qiagen Taq DNA polymerase (Qiagen, Beijing, China) with the following cycling conditions: 5 min at 94°C, followed by 35 cycles of 50 s at 94°C, 30 s at 40–55°C, and 1–2 min at 72°C. .. These PCR products were detected via electrophoresis in a 1% agarose gel and stained with ethidium bromide.

    Article Title: Members of a Large Retroposon Family Are Determinants of Post-Transcriptional Gene Expression in Leishmania
    Article Snippet: Paragraph title: Plasmid construction and transfections. ... The full-length 3′UTR of LmjF36.3810 and LmjF08.1270 transcripts from the termination codon to 434 bp beyond the poly(A) site in the case of LmjF36.3810, and to 84 bp beyond the poly(A) site in the case of LmjF08.1270, or the LmSIDER2 element or the 3′UTR lacking LmSIDER2, were amplified by PCR using Taq DNA polymerase (Qiagen, http://www.qiagen.com/ ) and primers with inserted BamHI or PstI restriction sites (see ).

    Article Title: Knockdown resistance in Anopheles vagus, An. sinensis, An. paraliae and An. peditaeniatus populations of the Mekong region
    Article Snippet: Plasmid and direct PCR sequencing were done by the VIB genetic service facility (University of Antwerp, Belgium) and aligned with ClustalW version 1.3 [ ]. .. All AS-PCR assays were performed in a 50 μl reaction mixture containing: 1 × Qiagen PCR buffer, 1 × Q solution, 0.5 mM MgCl2 , 200 μM dNTP's, 400 nM of the outer forward and reverse primer, 500 nM of the inner resistant and sensitive primer (Figure ), 1 unit Taq DNA polymerase (Qiagen, Hilden, Germany) and 1 μl template.

    Software:

    Article Title: The invasive New Guinea flatworm Platydemus manokwari in France, the first record for Europe: time for action is now
    Article Snippet: The PCR reaction was performed in 20 µl, containing 1 ng of DNA, 1× CoralLoad PCR buffer, 3 Mm MgCl2, 66 µM of each dNTP, 0.15 µM of each primer, and 0.5 units of Taq DNA polymerase (Qiagen). .. PCR products were purified and sequenced in both directions on 3730xl DNA Analyzer 96-capillary sequencer (Applied Biosystems).

    Electrophoresis:

    Article Title: Ancestral Gene Organization in the Mitochondrial Genome of Thyridosmylus langii (McLachlan, 1870) (Neuroptera: Osmylidae) and Implications for Lacewing Evolution
    Article Snippet: Short PCRs were conducted using Qiagen Taq DNA polymerase (Qiagen, Beijing, China) with the following cycling conditions: 5 min at 94°C, followed by 35 cycles of 50 s at 94°C, 30 s at 40–55°C, and 1–2 min at 72°C. .. Short PCRs were conducted using Qiagen Taq DNA polymerase (Qiagen, Beijing, China) with the following cycling conditions: 5 min at 94°C, followed by 35 cycles of 50 s at 94°C, 30 s at 40–55°C, and 1–2 min at 72°C.

    Article Title: Identification of Key Uric Acid Synthesis Pathway in a Unique Mutant Silkworm Bombyx mori Model of Parkinson's Disease
    Article Snippet: Total RNA from 500 brains of day 3–5 fifth instar larvae were treated with DNase and processed for cDNA synthesis using primers of 12 to 18 oligo(dT) and SuperScript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA). cDNA was amplified by PCR using Taq DNA polymerase (Qiagen) with the primers for tyrosine hydroxylase (TH; 5′-ACACAACAGTGGTTCAGTCG-3′ and 5′-AGGAGTCTCGCAATGTACAC-3′ ), actin ( 5′-TATCGCCGACAGGATGCAGAAGGA-3′ and 5′-TAGAAGCACTTGCGGTGAACGATG-3′ ) and DJ-1 ( 5′-CATTTGTGCTGCTTCCATAGCGTT-3′ and 5′-CATTCCCTTTTCGACTTGATCGGC-3′ ), as described previously . .. RT-PCR for B. mori actin was used as a positive loading control.

    Article Title: Quality not Quantity: The Role of Marine Natural Products in Drug Discovery and Reverse Chemical Proteomics
    Article Snippet: Taq DNA polymerase and QIAquick PCR purification kits were obtained from QIAGEN (USA). .. Taq DNA polymerase and QIAquick PCR purification kits were obtained from QIAGEN (USA).

    Article Title: Silencing and Un-silencing of Tetracycline-Controlled Genes in Neurons
    Article Snippet: PCRs were performed on MJ Research thermocyclers (Waltham, Massachusetts, United States) in a final volume of 25 µl containing 1× PCR Buffer, 1 U /Taq/ DNA polymerase (Qiagen), 200 µM dNTPs, 12.5 pmol each of forward and reverse primers, and 7 ng of bisulphite-treated genomic DNA. .. PCRs were performed on MJ Research thermocyclers (Waltham, Massachusetts, United States) in a final volume of 25 µl containing 1× PCR Buffer, 1 U /Taq/ DNA polymerase (Qiagen), 200 µM dNTPs, 12.5 pmol each of forward and reverse primers, and 7 ng of bisulphite-treated genomic DNA.

    Negative Control:

    Article Title: Impairment of germline transmission after blastocyst injection with murine embryonic stem cells cultured with mouse hepatitis virus and mouse minute virus
    Article Snippet: A double-distilled water sample was used as a negative control in both the RT and the PCR steps. .. PCR was performed in a total volume of 20 μl using Taq DNA polymerase (Qiagen) for 35 cycles in a thermocycler (Biometra, Biomedizinische Analytik GmbH, Göttingen, Germany).

    Agarose Gel Electrophoresis:

    Article Title: Ancestral Gene Organization in the Mitochondrial Genome of Thyridosmylus langii (McLachlan, 1870) (Neuroptera: Osmylidae) and Implications for Lacewing Evolution
    Article Snippet: Short PCRs were conducted using Qiagen Taq DNA polymerase (Qiagen, Beijing, China) with the following cycling conditions: 5 min at 94°C, followed by 35 cycles of 50 s at 94°C, 30 s at 40–55°C, and 1–2 min at 72°C. .. Short PCRs were conducted using Qiagen Taq DNA polymerase (Qiagen, Beijing, China) with the following cycling conditions: 5 min at 94°C, followed by 35 cycles of 50 s at 94°C, 30 s at 40–55°C, and 1–2 min at 72°C.

    Article Title: Identification of Key Uric Acid Synthesis Pathway in a Unique Mutant Silkworm Bombyx mori Model of Parkinson's Disease
    Article Snippet: Total RNA from 500 brains of day 3–5 fifth instar larvae were treated with DNase and processed for cDNA synthesis using primers of 12 to 18 oligo(dT) and SuperScript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA). cDNA was amplified by PCR using Taq DNA polymerase (Qiagen) with the primers for tyrosine hydroxylase (TH; 5′-ACACAACAGTGGTTCAGTCG-3′ and 5′-AGGAGTCTCGCAATGTACAC-3′ ), actin ( 5′-TATCGCCGACAGGATGCAGAAGGA-3′ and 5′-TAGAAGCACTTGCGGTGAACGATG-3′ ) and DJ-1 ( 5′-CATTTGTGCTGCTTCCATAGCGTT-3′ and 5′-CATTCCCTTTTCGACTTGATCGGC-3′ ), as described previously . .. RT-PCR for B. mori actin was used as a positive loading control.

    Article Title: Knockdown resistance in Anopheles vagus, An. sinensis, An. paraliae and An. peditaeniatus populations of the Mekong region
    Article Snippet: All AS-PCR assays were performed in a 50 μl reaction mixture containing: 1 × Qiagen PCR buffer, 1 × Q solution, 0.5 mM MgCl2 , 200 μM dNTP's, 400 nM of the outer forward and reverse primer, 500 nM of the inner resistant and sensitive primer (Figure ), 1 unit Taq DNA polymerase (Qiagen, Hilden, Germany) and 1 μl template. .. The cycling conditions described above for the amplification of the DIIS6 region were used.

    In Vitro:

    Article Title: Faithful transcription initiation from a mitochondrial promoter in transgenic plastids
    Article Snippet: Paragraph title: 5′-end mapping of in vivo and in vitro -synthesized RNAs ... The products of reverse transcription (RT) were amplified in a first PCR step by using 4 µl of the RT reaction, 5 pmol of both the adapter-specific forward primer P1a (5′-CGAATTCCTGTAGAACGAACACTAGAAG-3′) and the gene-specific reverse primer Pb (5′-ATAGTTTGTGGAGAATTTCGCTTCC-3′), 200 µM of each dNTP and 0.5 U of Taq DNA polymerase (Qiagen) in 25 µl of the appropriate buffer.

    Chromosome Walking:

    Article Title: Ancestral Gene Organization in the Mitochondrial Genome of Thyridosmylus langii (McLachlan, 1870) (Neuroptera: Osmylidae) and Implications for Lacewing Evolution
    Article Snippet: Short PCRs were conducted using Qiagen Taq DNA polymerase (Qiagen, Beijing, China) with the following cycling conditions: 5 min at 94°C, followed by 35 cycles of 50 s at 94°C, 30 s at 40–55°C, and 1–2 min at 72°C. .. These PCR products were detected via electrophoresis in a 1% agarose gel and stained with ethidium bromide.

    Spectrophotometry:

    Article Title: Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans
    Article Snippet: Extracted genomic DNA was quantified using a NanoDrop ND1000 spectrophotometer (Peqlab; Erlangen, Germany). .. PCR amplification of genomic DNA was carried out using the HotMasterMix 2.5x from 5 PRIME (Hamburg, Germany) according to the manufacturer’s protocol or the Taq DNA polymerase from Qiagen (Hilden, Germany) in reaction buffer containing 200 μM (each) deoxynucleotide triphosphates (dNTPs), 1 μM (each) oligonucleotide primers and ca.

    Article Title: Fractalkine (CX3CL1), GM-CSF and VEGF-a levels are reduced by statins in adult patients
    Article Snippet: RNA quality was assessed by OD260/280 using a Nanodrop spectrophotometer. .. Real-time PCR analysis was performed using SYBR green and Taq DNA polymerase (Qiagen) on a Bio-Rad CFX96 Connect Real-Time PCR Detection System.

    Sampling:

    Article Title: Y-chromosome phylogeny in the evolutionary net of chamois (genus Rupicapra)
    Article Snippet: Paragraph title: Sampling, DNA extraction and sex determination ... Reactions were performed in a final volume of 20 μl containing 2 μl (≈ 40-70 ng) DNA, 0.5 mM of each primer, 1x PCR Buffer, 200 mM of each dNTP, 2.5 mM MgCl2 and 1 U of Taq DNA polymerase (Qiagen, Hilden, Germany).

    Concentration Assay:

    Article Title: Impairment of germline transmission after blastocyst injection with murine embryonic stem cells cultured with mouse hepatitis virus and mouse minute virus
    Article Snippet: Immediately thereafter, an RNase inhibitor (MBI Fermentas, St. Leon-Rot, Germany) was added to the RT mixture at a concentration of 10 U/μl. cDNA was synthesized using 12 μl RNA and the Omniscript Reverse Transcriptase kit (Qiagen). .. PCR was performed in a total volume of 20 μl using Taq DNA polymerase (Qiagen) for 35 cycles in a thermocycler (Biometra, Biomedizinische Analytik GmbH, Göttingen, Germany).

    DNA Purification:

    Article Title: Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans
    Article Snippet: For the isolation of genomic DNA from strain Ivo14T and further reference strains the MasterPure™ Gram Positive DNA Purification Kit from Epicentre (Madison, USA) was used according to the instructions provided by the manufacturer. .. PCR amplification of genomic DNA was carried out using the HotMasterMix 2.5x from 5 PRIME (Hamburg, Germany) according to the manufacturer’s protocol or the Taq DNA polymerase from Qiagen (Hilden, Germany) in reaction buffer containing 200 μM (each) deoxynucleotide triphosphates (dNTPs), 1 μM (each) oligonucleotide primers and ca.

    Staining:

    Article Title: Ancestral Gene Organization in the Mitochondrial Genome of Thyridosmylus langii (McLachlan, 1870) (Neuroptera: Osmylidae) and Implications for Lacewing Evolution
    Article Snippet: Short PCRs were conducted using Qiagen Taq DNA polymerase (Qiagen, Beijing, China) with the following cycling conditions: 5 min at 94°C, followed by 35 cycles of 50 s at 94°C, 30 s at 40–55°C, and 1–2 min at 72°C. .. Short PCRs were conducted using Qiagen Taq DNA polymerase (Qiagen, Beijing, China) with the following cycling conditions: 5 min at 94°C, followed by 35 cycles of 50 s at 94°C, 30 s at 40–55°C, and 1–2 min at 72°C.

    Article Title: Identification of Key Uric Acid Synthesis Pathway in a Unique Mutant Silkworm Bombyx mori Model of Parkinson's Disease
    Article Snippet: Total RNA from 500 brains of day 3–5 fifth instar larvae were treated with DNase and processed for cDNA synthesis using primers of 12 to 18 oligo(dT) and SuperScript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA). cDNA was amplified by PCR using Taq DNA polymerase (Qiagen) with the primers for tyrosine hydroxylase (TH; 5′-ACACAACAGTGGTTCAGTCG-3′ and 5′-AGGAGTCTCGCAATGTACAC-3′ ), actin ( 5′-TATCGCCGACAGGATGCAGAAGGA-3′ and 5′-TAGAAGCACTTGCGGTGAACGATG-3′ ) and DJ-1 ( 5′-CATTTGTGCTGCTTCCATAGCGTT-3′ and 5′-CATTCCCTTTTCGACTTGATCGGC-3′ ), as described previously . .. RT-PCR for B. mori actin was used as a positive loading control.

    Article Title: Knockdown resistance in Anopheles vagus, An. sinensis, An. paraliae and An. peditaeniatus populations of the Mekong region
    Article Snippet: All AS-PCR assays were performed in a 50 μl reaction mixture containing: 1 × Qiagen PCR buffer, 1 × Q solution, 0.5 mM MgCl2 , 200 μM dNTP's, 400 nM of the outer forward and reverse primer, 500 nM of the inner resistant and sensitive primer (Figure ), 1 unit Taq DNA polymerase (Qiagen, Hilden, Germany) and 1 μl template. .. The cycling conditions described above for the amplification of the DIIS6 region were used.

    Variant Assay:

    Article Title: Identification of More Than Two Paternal Haplotypes of the Ovine Fatty Acid-Binding Protein 4 (FABP4) Gene in Half-Sib Families: Evidence of Intragenic Meiotic Recombination
    Article Snippet: Amplifications were performed in a 15-µL reaction containing the DNA on one 1.2 mm punch of FTA card, 0.25 µM of each paired primer set, 150 µM dNTPs (Bioline, London, UK), 2.5 mM Mg2+ , 0.5 U Taq DNA polymerase (Qiagen, Hilden, Germany) and 1× the reaction buffer supplied with the enzyme. .. After denaturation and rapid cooling on wet ice, PCR amplicons were electrophoresed in 14% (for region 1), or 10% (for region 2) polyacrylamide gels for 19 hours in 0.5×TBE at 7.5°C and 390 V (for region 1) or 300 V (for region 2).

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    Qiagen high quality taq polymerases qiagen taq dna polymerase
    High Quality Taq Polymerases Qiagen Taq Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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