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Fisher Scientific high pressure liquid chromatography hplc grade
High Pressure Liquid Chromatography Hplc Grade, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/high pressure liquid chromatography hplc grade/product/Fisher Scientific
Average 88 stars, based on 9 article reviews
Price from $9.99 to $1999.99
high pressure liquid chromatography hplc grade - by Bioz Stars, 2021-01
88/100 stars

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High Performance Liquid Chromatography:

Article Title: Determination of Total Chlorogenic Acids in Commercial Green Coffee Extracts
Article Snippet: .. Methanol and acetonitrile were high-pressure liquid chromatography (HPLC) grade and obtained from Fisher Chemical. .. Formic acid was reagent grade (Sigma) and was mixed at the appropriate concentration in water.

Article Title: Modulation of Arachidonic Acid Metabolism in the Rat Kidney by Sulforaphane: Implications for Regulation of Blood Pressure
Article Snippet: .. Acetonitrile and methanol were high pressure liquid chromatography (HPLC) grade and obtained from Fisher Scientific (Pittsburg, PA). .. Animals Male 14-week-old spontaneously hypertensive rats (SHR) were obtained from Charles River Laboratories (Wilmington, MA).

Article Title: Bioavailability of (Geno)toxic Contaminants in Polycyclic Aromatic Hydrocarbon–Contaminated Soil Before and After Biological Treatment
Article Snippet: .. All solvents were high-pressure liquid chromatography (HPLC) grade and were purchased from Fisher Scientific (Pittsburgh, PA). .. Tenax TA beads (60/80 mesh) and the universal (mixed-function) Oasis HLB resin (particle size 60 μm) were obtained from Alltech (Deerfield, IL) and Waters (Milford, MA), respectively.

Article Title: Induction of Apoptosis by Sphingoid Long-Chain Bases in Aspergillus nidulans
Article Snippet: .. All the organic solvents were high-pressure liquid chromatography (HPLC) grade and were purchased from Fisher Scientific (Cincinnati, Ohio). .. Pulse-labeling of sphingolipids with [4,5-3 H]DHS-1-P and [4,5-3 H]DHS, extraction, and analysis by thin-layer chromatography were performed as previously described ( , , ).

Article Title: CYP2J2 and CYP2C19 Are the Major Enzymes Responsible for Metabolism of Albendazole and Fenbendazole in Human Liver Microsomes and Recombinant P450 Assay Systems
Article Snippet: .. Solvents were high-pressure liquid chromatography (HPLC) grade (Fisher Scientific Co., Pittsburgh, PA), and the other chemicals were of the highest quality available. .. Pooled human liver microsomes (H161), human liver microsomes (HLMs) from 14 different single donors, and 10 human recombinant P450 isoforms (1A2, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, 2J2, and 3A4; Supersomes) were purchased from BD Biosciences (San Jose, CA).

Article Title: Nutritional Value, Chemical Characterization and Bulb Morphology of Greek Garlic Landraces
Article Snippet: .. Acetonitrile (99.9%), n -hexane (95%) and ethyl acetate (99.8%) were High Pressure Liquid Chromatography (HPLC) grade from Fisher Scientific (Lisbon, Portugal). .. The water was obtained from a purification system Millipore Direct-Q (TGI Pure Water Systems, Greenville, SC, USA).

Article Title: Desorption of polycyclic aromatic hydrocarbons from field-contaminated soil to a two-dimensional hydrophobic surface before and after bioremediation
Article Snippet: .. Solvents were high-pressure liquid chromatography (HPLC) grade and were obtained from Fisher Scientific (Pittsburgh, PA, USA). ..

Article Title: Nutritional Value, Chemical Characterization and Bulb Morphology of Greek Garlic Landraces
Article Snippet: .. Standards and Reagents Acetonitrile (99.9%), n -hexane (95%) and ethyl acetate (99.8%) were High Pressure Liquid Chromatography (HPLC) grade from Fisher Scientific (Lisbon, Portugal). .. The water was obtained from a purification system Millipore Direct-Q (TGI Pure Water Systems, Greenville, SC, USA).

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  • 92
    Fisher Scientific hplc grade acetonitrile
    Fraction analysis of adjacent fractions at beginning and end of product appearance following preparative reversed-phase (RP)-high-performance liquid chromatography <t>(HPLC)</t> of 100 mg (top panels) and 200 mg (bottom panels) of synthetic 26-residue amphipathic <t>\documentclass[12pt]{minimal}</t> \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$\alpha$\end{document} -helical antimicrobial peptide. Column, conditions of preparative RP-HPLC and fraction analysis, and peptide sequence described under Subheading 3.6.1 . P denotes desired product.
    Hplc Grade Acetonitrile, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 505 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc grade acetonitrile/product/Fisher Scientific
    Average 92 stars, based on 505 article reviews
    Price from $9.99 to $1999.99
    hplc grade acetonitrile - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    92
    Fisher Scientific hplc grade
    Purification of his-tagged OMT5 and determination of the reaction product for cyanidin 3- O -glucoside . (A) SDS-PAGE analysis of OMT5: total soluble protein from E. coli expressing OMT5 prior to induction with 100 lM IPTG (lane 1), 24 h post-induction (lane 2); IMAC-purified OMT5 (lane 3). (B) The OMT5 enzyme reaction as revealed by <t>HPLC-DAD</t> chromatograms at 520 nm (C). HPLC retention times: cyanidin 3- O -glucoside (Rt: 7.3 min); peonidin 3- O -glucoside (Rt: 8.6 min).
    Hplc Grade, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 670 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc grade/product/Fisher Scientific
    Average 92 stars, based on 670 article reviews
    Price from $9.99 to $1999.99
    hplc grade - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    88
    Fisher Scientific hplc grade grade chloroform
    Fatty acid analysis of lipid released from HMW O-Ag capsule by mild acid treatment. A) HPTLC of lipids recovered after mild acid hydrolysis of the indicated preparations. The solvent system used was CHCl 3 :Pyridine:88% Formic <t>Acid:MeOH:H</t> 2 O (54:46:16:0:5 v/v). The arrowhead indicates the dominant species in free lipid A. Highly enriched free lipid A was derived via Bligh-Dyer extraction of the EtOHp (see Fig 2F ). B) <t>HPLC</t> analysis of [ 14 C] fatty acids derived from free lipid A and lipid released by mild acid treatment of HMW O-Ag. C) The four major peaks in B were quantified and expressed as the % of total recovered cpm.
    Hplc Grade Grade Chloroform, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc grade grade chloroform/product/Fisher Scientific
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc grade grade chloroform - by Bioz Stars, 2021-01
    88/100 stars
      Buy from Supplier

    88
    Fisher Scientific nuclease free pbs
    To process and use tissues: we first fill a plastic cryo-mold half way with OCT, then we freeze the OCT partially (just the edges), place the tissue in center, cover tissue with OCT, freeze until opaque (this is most quickly performed in a dry-ice/ethanol bath, but we have successfully performed this on dry-ice alone as well). We cut sections on a cryostat (after cleaning cryostat with ethanol), and then we store the resulting slides at -80°C (and never let them thaw). Upon use, we take slides from -80°C fridge and place them either directly into 95% ethanol (with no <t>PBS</t> or water exposure at all), or, if pursuing IF-IHC before <t>LCM,</t> slides are placed directly into cold nuclease-free PBS + 0.1% Tween 20.
    Nuclease Free Pbs, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nuclease free pbs/product/Fisher Scientific
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nuclease free pbs - by Bioz Stars, 2021-01
    88/100 stars
      Buy from Supplier

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    Fraction analysis of adjacent fractions at beginning and end of product appearance following preparative reversed-phase (RP)-high-performance liquid chromatography (HPLC) of 100 mg (top panels) and 200 mg (bottom panels) of synthetic 26-residue amphipathic \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$\alpha$\end{document} -helical antimicrobial peptide. Column, conditions of preparative RP-HPLC and fraction analysis, and peptide sequence described under Subheading 3.6.1 . P denotes desired product.

    Journal: Peptide Characterization and Application Protocols

    Article Title: HPLC Analysis and Purification of Peptides

    doi: 10.1007/978-1-59745-430-8_1

    Figure Lengend Snippet: Fraction analysis of adjacent fractions at beginning and end of product appearance following preparative reversed-phase (RP)-high-performance liquid chromatography (HPLC) of 100 mg (top panels) and 200 mg (bottom panels) of synthetic 26-residue amphipathic \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$\alpha$\end{document} -helical antimicrobial peptide. Column, conditions of preparative RP-HPLC and fraction analysis, and peptide sequence described under Subheading 3.6.1 . P denotes desired product.

    Article Snippet: HPLC-grade acetonitrile (CH \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$_{3}$\end{document} CN) is obtained from Fisher Scientific (Pittsburgh, PA) or EM Science (Gibbstown, NJ).

    Techniques: High Performance Liquid Chromatography, Sequencing

    Pooled fractions following reversed-phase (RP)-high-performance liquid chromatography (HPLC) purification of 100 mg of crude synthetic 26-residue amphipathic \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$\alpha$\end{document} -helical antimicrobial peptide. Column, conditions, and peptide sequence described under Subheading 3.6.1 . Analytical RP-HPLC elution profiles of pooled fractions: (A) pool of all fractions containing hydrophilic impurities, (B) pool of all fractions containing pure product, denoted P, and ( C) pool of all fractions containing hydrophobic impurities.

    Journal: Peptide Characterization and Application Protocols

    Article Title: HPLC Analysis and Purification of Peptides

    doi: 10.1007/978-1-59745-430-8_1

    Figure Lengend Snippet: Pooled fractions following reversed-phase (RP)-high-performance liquid chromatography (HPLC) purification of 100 mg of crude synthetic 26-residue amphipathic \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$\alpha$\end{document} -helical antimicrobial peptide. Column, conditions, and peptide sequence described under Subheading 3.6.1 . Analytical RP-HPLC elution profiles of pooled fractions: (A) pool of all fractions containing hydrophilic impurities, (B) pool of all fractions containing pure product, denoted P, and ( C) pool of all fractions containing hydrophobic impurities.

    Article Snippet: HPLC-grade acetonitrile (CH \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$_{3}$\end{document} CN) is obtained from Fisher Scientific (Pittsburgh, PA) or EM Science (Gibbstown, NJ).

    Techniques: High Performance Liquid Chromatography, Purification, Sequencing

    Purification of his-tagged OMT5 and determination of the reaction product for cyanidin 3- O -glucoside . (A) SDS-PAGE analysis of OMT5: total soluble protein from E. coli expressing OMT5 prior to induction with 100 lM IPTG (lane 1), 24 h post-induction (lane 2); IMAC-purified OMT5 (lane 3). (B) The OMT5 enzyme reaction as revealed by HPLC-DAD chromatograms at 520 nm (C). HPLC retention times: cyanidin 3- O -glucoside (Rt: 7.3 min); peonidin 3- O -glucoside (Rt: 8.6 min).

    Journal: BMC Genomics

    Article Title: Combined analysis of transcriptome and metabolite data reveals extensive differences between black and brown nearly-isogenic soybean (Glycine max) seed coats enabling the identification of pigment isogenes

    doi: 10.1186/1471-2164-12-381

    Figure Lengend Snippet: Purification of his-tagged OMT5 and determination of the reaction product for cyanidin 3- O -glucoside . (A) SDS-PAGE analysis of OMT5: total soluble protein from E. coli expressing OMT5 prior to induction with 100 lM IPTG (lane 1), 24 h post-induction (lane 2); IMAC-purified OMT5 (lane 3). (B) The OMT5 enzyme reaction as revealed by HPLC-DAD chromatograms at 520 nm (C). HPLC retention times: cyanidin 3- O -glucoside (Rt: 7.3 min); peonidin 3- O -glucoside (Rt: 8.6 min).

    Article Snippet: All solvents for LC-MS/MS and HPLC-DAD analyses were of HPLC grade purchased from Fisher Scientific (Ottawa, ON, Canada).

    Techniques: Purification, SDS Page, Expressing, High Performance Liquid Chromatography

    Purification of his-tagged UGT78K2 and determination of the reaction product and kinetics for cyanidin . (A) SDS-PAGE analysis of UGT78K2: total soluble protein from E. coli expressing UGT78K2 prior to induction with 100 lM IPTG (lane 1), 24 h post-induction (lane 2); IMAC-purified UGT78K2 (lane 3). (B) The UGT78K2 enzyme reaction as revealed by HPLC-DAD chromatograms at 520 nm (C). HPLC retention times: cyanidin (Rt: 10.1 min); cyanidin 3- O -glucoside (Rt: 7.1 min). (D) UGT78K2 kinetics for the cyanidin acceptor substrate using 5 mM UDPG as a sugar donor. Points represent the mean of three assays ± the standard deviation.

    Journal: BMC Genomics

    Article Title: Combined analysis of transcriptome and metabolite data reveals extensive differences between black and brown nearly-isogenic soybean (Glycine max) seed coats enabling the identification of pigment isogenes

    doi: 10.1186/1471-2164-12-381

    Figure Lengend Snippet: Purification of his-tagged UGT78K2 and determination of the reaction product and kinetics for cyanidin . (A) SDS-PAGE analysis of UGT78K2: total soluble protein from E. coli expressing UGT78K2 prior to induction with 100 lM IPTG (lane 1), 24 h post-induction (lane 2); IMAC-purified UGT78K2 (lane 3). (B) The UGT78K2 enzyme reaction as revealed by HPLC-DAD chromatograms at 520 nm (C). HPLC retention times: cyanidin (Rt: 10.1 min); cyanidin 3- O -glucoside (Rt: 7.1 min). (D) UGT78K2 kinetics for the cyanidin acceptor substrate using 5 mM UDPG as a sugar donor. Points represent the mean of three assays ± the standard deviation.

    Article Snippet: All solvents for LC-MS/MS and HPLC-DAD analyses were of HPLC grade purchased from Fisher Scientific (Ottawa, ON, Canada).

    Techniques: Purification, SDS Page, Expressing, High Performance Liquid Chromatography, Standard Deviation

    Anthocyanins from the seed coat of black ( iRT ) soybean variety Clark . Numbers correspond to chromatographic peaks identified by HPLC-DAD (see Table 1).

    Journal: BMC Genomics

    Article Title: Combined analysis of transcriptome and metabolite data reveals extensive differences between black and brown nearly-isogenic soybean (Glycine max) seed coats enabling the identification of pigment isogenes

    doi: 10.1186/1471-2164-12-381

    Figure Lengend Snippet: Anthocyanins from the seed coat of black ( iRT ) soybean variety Clark . Numbers correspond to chromatographic peaks identified by HPLC-DAD (see Table 1).

    Article Snippet: All solvents for LC-MS/MS and HPLC-DAD analyses were of HPLC grade purchased from Fisher Scientific (Ottawa, ON, Canada).

    Techniques: High Performance Liquid Chromatography

    Fatty acid analysis of lipid released from HMW O-Ag capsule by mild acid treatment. A) HPTLC of lipids recovered after mild acid hydrolysis of the indicated preparations. The solvent system used was CHCl 3 :Pyridine:88% Formic Acid:MeOH:H 2 O (54:46:16:0:5 v/v). The arrowhead indicates the dominant species in free lipid A. Highly enriched free lipid A was derived via Bligh-Dyer extraction of the EtOHp (see Fig 2F ). B) HPLC analysis of [ 14 C] fatty acids derived from free lipid A and lipid released by mild acid treatment of HMW O-Ag. C) The four major peaks in B were quantified and expressed as the % of total recovered cpm.

    Journal: PLoS ONE

    Article Title: Evidence Suggesting That Francisella tularensis O-Antigen Capsule Contains a Lipid A-Like Molecule That Is Structurally Distinct from the More Abundant Free Lipid A

    doi: 10.1371/journal.pone.0157842

    Figure Lengend Snippet: Fatty acid analysis of lipid released from HMW O-Ag capsule by mild acid treatment. A) HPTLC of lipids recovered after mild acid hydrolysis of the indicated preparations. The solvent system used was CHCl 3 :Pyridine:88% Formic Acid:MeOH:H 2 O (54:46:16:0:5 v/v). The arrowhead indicates the dominant species in free lipid A. Highly enriched free lipid A was derived via Bligh-Dyer extraction of the EtOHp (see Fig 2F ). B) HPLC analysis of [ 14 C] fatty acids derived from free lipid A and lipid released by mild acid treatment of HMW O-Ag. C) The four major peaks in B were quantified and expressed as the % of total recovered cpm.

    Article Snippet: HPLC-grade grade chloroform (CHCl3 ) and methanol (MeOH) were obtained from Fisher Scientific (Pittsburgh, PA).

    Techniques: High Performance Thin Layer Chromatography, Derivative Assay, High Performance Liquid Chromatography

    To process and use tissues: we first fill a plastic cryo-mold half way with OCT, then we freeze the OCT partially (just the edges), place the tissue in center, cover tissue with OCT, freeze until opaque (this is most quickly performed in a dry-ice/ethanol bath, but we have successfully performed this on dry-ice alone as well). We cut sections on a cryostat (after cleaning cryostat with ethanol), and then we store the resulting slides at -80°C (and never let them thaw). Upon use, we take slides from -80°C fridge and place them either directly into 95% ethanol (with no PBS or water exposure at all), or, if pursuing IF-IHC before LCM, slides are placed directly into cold nuclease-free PBS + 0.1% Tween 20.

    Journal: Biological Procedures Online

    Article Title: New quick method for isolating RNA from laser captured cells stained by immunofluorescent immunohistochemistry; RNA suitable for direct use in fluorogenic TaqMan one-step real-time RT-PCR

    doi: 10.1251/bpo107

    Figure Lengend Snippet: To process and use tissues: we first fill a plastic cryo-mold half way with OCT, then we freeze the OCT partially (just the edges), place the tissue in center, cover tissue with OCT, freeze until opaque (this is most quickly performed in a dry-ice/ethanol bath, but we have successfully performed this on dry-ice alone as well). We cut sections on a cryostat (after cleaning cryostat with ethanol), and then we store the resulting slides at -80°C (and never let them thaw). Upon use, we take slides from -80°C fridge and place them either directly into 95% ethanol (with no PBS or water exposure at all), or, if pursuing IF-IHC before LCM, slides are placed directly into cold nuclease-free PBS + 0.1% Tween 20.

    Article Snippet: Upon retrieval from -80°C for use in IF-IHC-LCM, slides are either treated as in Fig. (in cases where no IF-IHC procedure is to precede LCM) or, they are quickly transferred into cold nuclease-free PBS (made with HPLC-grade water, Fisher Scientific, Hanover, IL, PBS tablets and 0.1% Tween 20, Sigma, St. Louis, MO) within 30 seconds of their removal from the freezer (apparently, RNA degradation sets in very rapidly if this particular maneuver is not performed quickly; according to an Arcturus field technician).

    Techniques: Immunohistochemistry, Laser Capture Microdissection