high performance tlc hptlc aluminium backed silica gel 60 plates  (Merck KGaA)

 
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    Structured Review

    Merck KGaA high performance tlc hptlc aluminium backed silica gel 60 plates
    <t>HPTLC-separated</t> glycolipids and overlay analysis of the binding of purified F1C fimbriae. Chromatograms of glycolipids separated on HPTLC silica <t>gel</t> 60 and stained with orcinol (A and C), probed with purified F1C fimbriae, and immunostained with anti-fimbrial antibodies and horseradish peroxidase-conjugated anti-chicken antibodies (B and D) are shown. (A) Lane 1, (from top to bottom) glucosylceramide (4 μg, GlcCer), sulfatide (4 μg, SFT), asialo-GM 2 (GgO 3 Cer) (2 μg), and sialylated GM 1 (4 μg, GM1); lane 2, (from top to bottom), galactosylceramide 2 (4 μg, GalCer2), lactosylceramide (3 μg, LacCer), paragloboside (1 μg, nLc 4 Cer), and asialo-GM 1 (GgO 4 Cer) (2 μg). (B) Lane 1, fimbrial binding to GlcCer (upper double band) and to asialo-GM 2 (GgO 3 Cer) (lower double band); lane 2, fimbrial binding from top to bottom, GalCer2, LacCer, nLc 4 Cer, and asialo-GM 1 (GgO 4 Cer). (C) Lane contains (from top to bottom) globotriaosylceramide (4 μg, Gb 3 Cer) and globotetraosylceramide (4 μg, Gb 4 Cer). (D) Lane contains fimbriae bound to globotriaosylceramide (Gb 3 Cer).
    High Performance Tlc Hptlc Aluminium Backed Silica Gel 60 Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high performance tlc hptlc aluminium backed silica gel 60 plates/product/Merck KGaA
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high performance tlc hptlc aluminium backed silica gel 60 plates - by Bioz Stars, 2020-10
    80/100 stars

    Images

    1) Product Images from "Receptor Structure for F1C Fimbriae of Uropathogenic Escherichia coli"

    Article Title: Receptor Structure for F1C Fimbriae of Uropathogenic Escherichia coli

    Journal: Infection and Immunity

    doi:

    HPTLC-separated glycolipids and overlay analysis of the binding of purified F1C fimbriae. Chromatograms of glycolipids separated on HPTLC silica gel 60 and stained with orcinol (A and C), probed with purified F1C fimbriae, and immunostained with anti-fimbrial antibodies and horseradish peroxidase-conjugated anti-chicken antibodies (B and D) are shown. (A) Lane 1, (from top to bottom) glucosylceramide (4 μg, GlcCer), sulfatide (4 μg, SFT), asialo-GM 2 (GgO 3 Cer) (2 μg), and sialylated GM 1 (4 μg, GM1); lane 2, (from top to bottom), galactosylceramide 2 (4 μg, GalCer2), lactosylceramide (3 μg, LacCer), paragloboside (1 μg, nLc 4 Cer), and asialo-GM 1 (GgO 4 Cer) (2 μg). (B) Lane 1, fimbrial binding to GlcCer (upper double band) and to asialo-GM 2 (GgO 3 Cer) (lower double band); lane 2, fimbrial binding from top to bottom, GalCer2, LacCer, nLc 4 Cer, and asialo-GM 1 (GgO 4 Cer). (C) Lane contains (from top to bottom) globotriaosylceramide (4 μg, Gb 3 Cer) and globotetraosylceramide (4 μg, Gb 4 Cer). (D) Lane contains fimbriae bound to globotriaosylceramide (Gb 3 Cer).
    Figure Legend Snippet: HPTLC-separated glycolipids and overlay analysis of the binding of purified F1C fimbriae. Chromatograms of glycolipids separated on HPTLC silica gel 60 and stained with orcinol (A and C), probed with purified F1C fimbriae, and immunostained with anti-fimbrial antibodies and horseradish peroxidase-conjugated anti-chicken antibodies (B and D) are shown. (A) Lane 1, (from top to bottom) glucosylceramide (4 μg, GlcCer), sulfatide (4 μg, SFT), asialo-GM 2 (GgO 3 Cer) (2 μg), and sialylated GM 1 (4 μg, GM1); lane 2, (from top to bottom), galactosylceramide 2 (4 μg, GalCer2), lactosylceramide (3 μg, LacCer), paragloboside (1 μg, nLc 4 Cer), and asialo-GM 1 (GgO 4 Cer) (2 μg). (B) Lane 1, fimbrial binding to GlcCer (upper double band) and to asialo-GM 2 (GgO 3 Cer) (lower double band); lane 2, fimbrial binding from top to bottom, GalCer2, LacCer, nLc 4 Cer, and asialo-GM 1 (GgO 4 Cer). (C) Lane contains (from top to bottom) globotriaosylceramide (4 μg, Gb 3 Cer) and globotetraosylceramide (4 μg, Gb 4 Cer). (D) Lane contains fimbriae bound to globotriaosylceramide (Gb 3 Cer).

    Techniques Used: High Performance Thin Layer Chromatography, Binding Assay, Purification, Staining

    Related Articles

    High Performance Thin Layer Chromatography:

    Article Title: Receptor Structure for F1C Fimbriae of Uropathogenic Escherichia coli
    Article Snippet: .. Briefly, pure glycolipids (Sigma) were separated for analytical purpose on high-performance TLC (HPTLC) aluminium-backed silica gel 60 plates (Merck, Darmstadt, Germany). .. Glycolipids were stained with orcinol spray (Sigma).

    Thin Layer Chromatography:

    Article Title: Receptor Structure for F1C Fimbriae of Uropathogenic Escherichia coli
    Article Snippet: .. Briefly, pure glycolipids (Sigma) were separated for analytical purpose on high-performance TLC (HPTLC) aluminium-backed silica gel 60 plates (Merck, Darmstadt, Germany). .. Glycolipids were stained with orcinol spray (Sigma).

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    Merck KGaA high performance tlc hptlc aluminium backed silica gel 60 plates
    <t>HPTLC-separated</t> glycolipids and overlay analysis of the binding of purified F1C fimbriae. Chromatograms of glycolipids separated on HPTLC silica <t>gel</t> 60 and stained with orcinol (A and C), probed with purified F1C fimbriae, and immunostained with anti-fimbrial antibodies and horseradish peroxidase-conjugated anti-chicken antibodies (B and D) are shown. (A) Lane 1, (from top to bottom) glucosylceramide (4 μg, GlcCer), sulfatide (4 μg, SFT), asialo-GM 2 (GgO 3 Cer) (2 μg), and sialylated GM 1 (4 μg, GM1); lane 2, (from top to bottom), galactosylceramide 2 (4 μg, GalCer2), lactosylceramide (3 μg, LacCer), paragloboside (1 μg, nLc 4 Cer), and asialo-GM 1 (GgO 4 Cer) (2 μg). (B) Lane 1, fimbrial binding to GlcCer (upper double band) and to asialo-GM 2 (GgO 3 Cer) (lower double band); lane 2, fimbrial binding from top to bottom, GalCer2, LacCer, nLc 4 Cer, and asialo-GM 1 (GgO 4 Cer). (C) Lane contains (from top to bottom) globotriaosylceramide (4 μg, Gb 3 Cer) and globotetraosylceramide (4 μg, Gb 4 Cer). (D) Lane contains fimbriae bound to globotriaosylceramide (Gb 3 Cer).
    High Performance Tlc Hptlc Aluminium Backed Silica Gel 60 Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high performance tlc hptlc aluminium backed silica gel 60 plates/product/Merck KGaA
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high performance tlc hptlc aluminium backed silica gel 60 plates - by Bioz Stars, 2020-10
    80/100 stars
      Buy from Supplier

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    HPTLC-separated glycolipids and overlay analysis of the binding of purified F1C fimbriae. Chromatograms of glycolipids separated on HPTLC silica gel 60 and stained with orcinol (A and C), probed with purified F1C fimbriae, and immunostained with anti-fimbrial antibodies and horseradish peroxidase-conjugated anti-chicken antibodies (B and D) are shown. (A) Lane 1, (from top to bottom) glucosylceramide (4 μg, GlcCer), sulfatide (4 μg, SFT), asialo-GM 2 (GgO 3 Cer) (2 μg), and sialylated GM 1 (4 μg, GM1); lane 2, (from top to bottom), galactosylceramide 2 (4 μg, GalCer2), lactosylceramide (3 μg, LacCer), paragloboside (1 μg, nLc 4 Cer), and asialo-GM 1 (GgO 4 Cer) (2 μg). (B) Lane 1, fimbrial binding to GlcCer (upper double band) and to asialo-GM 2 (GgO 3 Cer) (lower double band); lane 2, fimbrial binding from top to bottom, GalCer2, LacCer, nLc 4 Cer, and asialo-GM 1 (GgO 4 Cer). (C) Lane contains (from top to bottom) globotriaosylceramide (4 μg, Gb 3 Cer) and globotetraosylceramide (4 μg, Gb 4 Cer). (D) Lane contains fimbriae bound to globotriaosylceramide (Gb 3 Cer).

    Journal: Infection and Immunity

    Article Title: Receptor Structure for F1C Fimbriae of Uropathogenic Escherichia coli

    doi:

    Figure Lengend Snippet: HPTLC-separated glycolipids and overlay analysis of the binding of purified F1C fimbriae. Chromatograms of glycolipids separated on HPTLC silica gel 60 and stained with orcinol (A and C), probed with purified F1C fimbriae, and immunostained with anti-fimbrial antibodies and horseradish peroxidase-conjugated anti-chicken antibodies (B and D) are shown. (A) Lane 1, (from top to bottom) glucosylceramide (4 μg, GlcCer), sulfatide (4 μg, SFT), asialo-GM 2 (GgO 3 Cer) (2 μg), and sialylated GM 1 (4 μg, GM1); lane 2, (from top to bottom), galactosylceramide 2 (4 μg, GalCer2), lactosylceramide (3 μg, LacCer), paragloboside (1 μg, nLc 4 Cer), and asialo-GM 1 (GgO 4 Cer) (2 μg). (B) Lane 1, fimbrial binding to GlcCer (upper double band) and to asialo-GM 2 (GgO 3 Cer) (lower double band); lane 2, fimbrial binding from top to bottom, GalCer2, LacCer, nLc 4 Cer, and asialo-GM 1 (GgO 4 Cer). (C) Lane contains (from top to bottom) globotriaosylceramide (4 μg, Gb 3 Cer) and globotetraosylceramide (4 μg, Gb 4 Cer). (D) Lane contains fimbriae bound to globotriaosylceramide (Gb 3 Cer).

    Article Snippet: Briefly, pure glycolipids (Sigma) were separated for analytical purpose on high-performance TLC (HPTLC) aluminium-backed silica gel 60 plates (Merck, Darmstadt, Germany).

    Techniques: High Performance Thin Layer Chromatography, Binding Assay, Purification, Staining