high performance liquid chromatography  (Thermo Fisher)


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    Forma High Performance Chromatography Refrigerators
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    Benefit from the excellent temperature stability and full accessibility of Thermo Scientific Forma High Performance Chromatography Refrigerators designed for a variety of applications requiring close temperature control Our Forma chromatography refrigerators feature microprocessor control system positive airflow systems industrial quality cabinet construction and extra strength refrigeration compressors selected for industrial clinical and scientific use
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    Thermo Fisher high performance liquid chromatography
    Benefit from the excellent temperature stability and full accessibility of Thermo Scientific Forma High Performance Chromatography Refrigerators designed for a variety of applications requiring close temperature control Our Forma chromatography refrigerators feature microprocessor control system positive airflow systems industrial quality cabinet construction and extra strength refrigeration compressors selected for industrial clinical and scientific use
    https://www.bioz.com/result/high performance liquid chromatography/product/Thermo Fisher
    Average 99 stars, based on 232 article reviews
    Price from $9.99 to $1999.99
    high performance liquid chromatography - by Bioz Stars, 2020-07
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    High Performance Liquid Chromatography:

    Article Title: Discriminatory Components Retracing Strategy for Monitoring the Preparation Procedure of Chinese Patent Medicines by Fingerprint and Chemometric Analysis
    Article Snippet: .. In the structural elucidation, LTQ–Orbitrap mass spectrometer (Thermo Fisher Scientific) connected to ultra high-performance liquid chromatography instrument (Dionex Ultimated 3000) via an ESI source was applied in negative ion mode,with the mass range from 50 to1000 and the resolutions at 30000 for full scan and 7500 for MSn (n≥2). .. The chromatographic separation conditions were the same with those in HPLC–UV analysis.

    Article Title: APPLICATION OF PREPARATIVE HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY FOR SEPARATION OF ELATINE FROM DELPHINIUM SHAWURENSE
    Article Snippet: .. The high-performance liquid chromatography equipment (DIONEX, USA) used was a DIONEX system including a P680 pump, a ASI-100 automated sample injector, a TCC-100 temperature-controlled column compartment, a UVD170U detector. ..

    Article Title: Separation and Purification of Phenolic Acids and Myricetin from Black Currant by High Speed Countercurrent Chromatography
    Article Snippet: .. The high-performance liquid chromatography equipment (DIONEX, USA) used was a DIONEX system including a P680 pump, an ASI-100 Automated sample injector, a TCC-100 thermostatted column compartment, and a UVD170U detector. .. The analysis was carried out with an Inertsil ODS-SP column (5 µm, 4.6 × 250 mm GL Sciences Inc, Japan).

    Flow Cytometry:

    Article Title: Characterization of a Thermostable l-Arabinose (d-Galactose) Isomerase from the Hyperthermophilic Eubacterium Thermotoga maritima
    Article Snippet: .. High-performance ionic chromatography (Dionex) was carried out by using a Carbopac PA1 column (Dionex) equipped with a pulsed amperometry electrochemical detector. d -Galactose and d -tagatose were separated by isocratic elution in a 20 mM NaOH solution at a flow rate of 0.3 ml/min. .. The araA gene (1.5 kb) encoding AI was amplified by PCR from T. maritima genomic DNA and cloned into the pGEM-T Easy cloning vector.

    Mass Spectrometry:

    Article Title: Discriminatory Components Retracing Strategy for Monitoring the Preparation Procedure of Chinese Patent Medicines by Fingerprint and Chemometric Analysis
    Article Snippet: .. In the structural elucidation, LTQ–Orbitrap mass spectrometer (Thermo Fisher Scientific) connected to ultra high-performance liquid chromatography instrument (Dionex Ultimated 3000) via an ESI source was applied in negative ion mode,with the mass range from 50 to1000 and the resolutions at 30000 for full scan and 7500 for MSn (n≥2). .. The chromatographic separation conditions were the same with those in HPLC–UV analysis.

    Chromatography:

    Article Title: Characterization of a Thermostable l-Arabinose (d-Galactose) Isomerase from the Hyperthermophilic Eubacterium Thermotoga maritima
    Article Snippet: .. High-performance ionic chromatography (Dionex) was carried out by using a Carbopac PA1 column (Dionex) equipped with a pulsed amperometry electrochemical detector. d -Galactose and d -tagatose were separated by isocratic elution in a 20 mM NaOH solution at a flow rate of 0.3 ml/min. .. The araA gene (1.5 kb) encoding AI was amplified by PCR from T. maritima genomic DNA and cloned into the pGEM-T Easy cloning vector.

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    Thermo Fisher high performance liquid chromatography mass spectrometry hplc ms hplc programs
    Category of <t>HPLC-MS-identified</t> <t>glycylglycerins.</t> In human iPSCs, the glycylation of glycerin forms three different kinds of glycylglycerins, including monoglycylglycerins (MGG), diglycylglycerins (DGG) and triglycylglycerin (TGG). All these glycylglycerins carry positive charges in their amino groups at ≤pH 7.0 and can form isoforms as shown in the right bottom corner box.
    High Performance Liquid Chromatography Mass Spectrometry Hplc Ms Hplc Programs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
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    88
    Thermo Fisher ultra performance liquid chromatography high resolution mass spectrometry uplc hrms
    Box and whisker plots representing the range of normalized relative intensities for significant metabolic changes in the BBD microbial community after 24 h exposure to 50 µg/L MC-LR. Data are from <t>UPLC-HRMS</t> measurement of triplicate samples. Significance was determined by a fold change > 1.5 and a p value of
    Ultra Performance Liquid Chromatography High Resolution Mass Spectrometry Uplc Hrms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high performance liquid chromatography electrospray ionization ion trap mass spectrometry
    Negative ion HPLC-ESI-IT-MS 2 analysis of hydroethanolic extract of P. torta leaves. Total ion chromatogram (a) ; extracted ion chromatograms for m/z 479 (b) ; m/z 449 (c) ; and m/z 463 (d) . HPLC-ESI-IT-MS 2 , high-performance liquid <t>chromatography–electrospray</t>
    High Performance Liquid Chromatography Electrospray Ionization Ion Trap Mass Spectrometry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high performance liquid chromatography ms
    Validation of IEC Napepld deletion. a Napepld mRNA expression in the jejunum, colon, liver and epididymal adipose tissue (EAT) in ND-fed WT and Napepld ∆IEC mice ( n = 8–10). b NAPE-PLD protein levels in the colon. Representative western-blot of NAPE-PLD and β-Actin ( n = 9–10). c Levels of N -acylethanolamines and 2-acylglycerols in small intestinal epithelial cells of ND-fed WT and Napepld ∆IEC mice ( n = 7–9) were determined by using <t>high-performance</t> <t>liquid</t> <t>chromatography-MS</t> using an LTQ Orbitrap mass spectrometer as described in the methods. Dark blue: WT ND mice, light blue: Napepld ∆IEC ND mice. Data are presented as the mean ± s.e.m. *, ** and *** indicate a significant difference versus WT ND (Respectively P
    High Performance Liquid Chromatography Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Category of HPLC-MS-identified glycylglycerins. In human iPSCs, the glycylation of glycerin forms three different kinds of glycylglycerins, including monoglycylglycerins (MGG), diglycylglycerins (DGG) and triglycylglycerin (TGG). All these glycylglycerins carry positive charges in their amino groups at ≤pH 7.0 and can form isoforms as shown in the right bottom corner box.

    Journal: Nucleic Acids Research

    Article Title: Novel glycylated sugar alcohols protect ESC-specific microRNAs from degradation in iPS cells

    doi: 10.1093/nar/gkw186

    Figure Lengend Snippet: Category of HPLC-MS-identified glycylglycerins. In human iPSCs, the glycylation of glycerin forms three different kinds of glycylglycerins, including monoglycylglycerins (MGG), diglycylglycerins (DGG) and triglycylglycerin (TGG). All these glycylglycerins carry positive charges in their amino groups at ≤pH 7.0 and can form isoforms as shown in the right bottom corner box.

    Article Snippet: Analyses of glycylglycerins using high performance liquid chromatography—mass spectrometry (HPLC-MS) HPLC programs were run by an Ultimate 3000 HPLC machine (Thermo Scientific, Waltham, MA, USA) with a DNAPac PA-100 column (BioLC Semi-Prep 9 × 250 mm) at a flow rate of 3.6 ml/min.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    HPLC detection of glycylglycerin production and glycylglycerin-bound pre-miRNA. ( A ) HPLC analyses showed that production of glycylglycerins can naturally occur in a pure chemical reaction between glycine and glycerin at pH 6.8 in the presence of L-ascorbic acid (vit.C), but not in the same reaction at pH 7.3. ( B ) HPLC peak of DGG/TGG-mixed pre-miR-302a was shifted from 12.23 min at pH 7.3 (unbound) to 1.76 min at pH 6.8 (DGG/TGG-bound), indicating a marked loss of pre-miRNA negative charges after binding with DGG/TGG. After HPLC separation at pH 6.8, the DGG/TGG-bound pre-miR-302a was collected from the 1.7–1.8 min section of HPLC fragments and further used for MS analyses, as shown in Figure 7A . ( C ) Chemical 3D modeling of electro-binding between DGG/TGG and pre-miRNA/siRNA suggested that the long strand structures of 1,3-diglycylglycerin and 1,2,3-triglycylglycerin may fit into the minor grooves of the pre-miRNA/siRNA double helix structures and hence bind to the negatively charged phosphodiester-linkage backbones (green shadow) located in the minor grooves of pre-miRNA/siRNA via the positively charged amino groups of DGG/TGG, so as to form a layer of protective coating for preserving pre-miRNA/siRNA integrity.

    Journal: Nucleic Acids Research

    Article Title: Novel glycylated sugar alcohols protect ESC-specific microRNAs from degradation in iPS cells

    doi: 10.1093/nar/gkw186

    Figure Lengend Snippet: HPLC detection of glycylglycerin production and glycylglycerin-bound pre-miRNA. ( A ) HPLC analyses showed that production of glycylglycerins can naturally occur in a pure chemical reaction between glycine and glycerin at pH 6.8 in the presence of L-ascorbic acid (vit.C), but not in the same reaction at pH 7.3. ( B ) HPLC peak of DGG/TGG-mixed pre-miR-302a was shifted from 12.23 min at pH 7.3 (unbound) to 1.76 min at pH 6.8 (DGG/TGG-bound), indicating a marked loss of pre-miRNA negative charges after binding with DGG/TGG. After HPLC separation at pH 6.8, the DGG/TGG-bound pre-miR-302a was collected from the 1.7–1.8 min section of HPLC fragments and further used for MS analyses, as shown in Figure 7A . ( C ) Chemical 3D modeling of electro-binding between DGG/TGG and pre-miRNA/siRNA suggested that the long strand structures of 1,3-diglycylglycerin and 1,2,3-triglycylglycerin may fit into the minor grooves of the pre-miRNA/siRNA double helix structures and hence bind to the negatively charged phosphodiester-linkage backbones (green shadow) located in the minor grooves of pre-miRNA/siRNA via the positively charged amino groups of DGG/TGG, so as to form a layer of protective coating for preserving pre-miRNA/siRNA integrity.

    Article Snippet: Analyses of glycylglycerins using high performance liquid chromatography—mass spectrometry (HPLC-MS) HPLC programs were run by an Ultimate 3000 HPLC machine (Thermo Scientific, Waltham, MA, USA) with a DNAPac PA-100 column (BioLC Semi-Prep 9 × 250 mm) at a flow rate of 3.6 ml/min.

    Techniques: High Performance Liquid Chromatography, Binding Assay, Mass Spectrometry, Preserving

    Box and whisker plots representing the range of normalized relative intensities for significant metabolic changes in the BBD microbial community after 24 h exposure to 50 µg/L MC-LR. Data are from UPLC-HRMS measurement of triplicate samples. Significance was determined by a fold change > 1.5 and a p value of

    Journal: Life

    Article Title: Ecology and Physiology of the Pathogenic Cyanobacterium Roseofilum reptotaenium

    doi: 10.3390/life4040968

    Figure Lengend Snippet: Box and whisker plots representing the range of normalized relative intensities for significant metabolic changes in the BBD microbial community after 24 h exposure to 50 µg/L MC-LR. Data are from UPLC-HRMS measurement of triplicate samples. Significance was determined by a fold change > 1.5 and a p value of

    Article Snippet: The metabolomes for each sample were then measured via ultra-performance liquid chromatography—high resolution mass spectrometry (UPLC-HRMS) on an Exactive Plus Orbitrap MS (Thermo Fisher, Bremen, Germany) fitted with an UltiMate 3000 UPLC (Dionex, Sunnyvale, CA, USA) using described methods [ ].

    Techniques: Whisker Assay

    Negative ion HPLC-ESI-IT-MS 2 analysis of hydroethanolic extract of P. torta leaves. Total ion chromatogram (a) ; extracted ion chromatograms for m/z 479 (b) ; m/z 449 (c) ; and m/z 463 (d) . HPLC-ESI-IT-MS 2 , high-performance liquid chromatography–electrospray

    Journal: Journal of Medicinal Food

    Article Title: Flavonoid Detection in Hydroethanolic Extract of Pouteria torta (Sapotaceae) Leaves by HPLC-DAD and the Determination of Its Mutagenic Activity

    doi: 10.1089/jmf.2013.0116

    Figure Lengend Snippet: Negative ion HPLC-ESI-IT-MS 2 analysis of hydroethanolic extract of P. torta leaves. Total ion chromatogram (a) ; extracted ion chromatograms for m/z 479 (b) ; m/z 449 (c) ; and m/z 463 (d) . HPLC-ESI-IT-MS 2 , high-performance liquid chromatography–electrospray

    Article Snippet: High-performance liquid chromatography–electrospray ionization-ion trap mass spectrometry (HPLC-ESI-IT-MS ) was performed on a HPLC coupled to a mass spectrometer LCQ Fleet (Thermo Scientific® , Madison, WI, USA) and equipped with a direct insertion deposition flow injection analyzer.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    Validation of IEC Napepld deletion. a Napepld mRNA expression in the jejunum, colon, liver and epididymal adipose tissue (EAT) in ND-fed WT and Napepld ∆IEC mice ( n = 8–10). b NAPE-PLD protein levels in the colon. Representative western-blot of NAPE-PLD and β-Actin ( n = 9–10). c Levels of N -acylethanolamines and 2-acylglycerols in small intestinal epithelial cells of ND-fed WT and Napepld ∆IEC mice ( n = 7–9) were determined by using high-performance liquid chromatography-MS using an LTQ Orbitrap mass spectrometer as described in the methods. Dark blue: WT ND mice, light blue: Napepld ∆IEC ND mice. Data are presented as the mean ± s.e.m. *, ** and *** indicate a significant difference versus WT ND (Respectively P

    Journal: Nature Communications

    Article Title: Intestinal epithelial N-acylphosphatidylethanolamine phospholipase D links dietary fat to metabolic adaptations in obesity and steatosis

    doi: 10.1038/s41467-018-08051-7

    Figure Lengend Snippet: Validation of IEC Napepld deletion. a Napepld mRNA expression in the jejunum, colon, liver and epididymal adipose tissue (EAT) in ND-fed WT and Napepld ∆IEC mice ( n = 8–10). b NAPE-PLD protein levels in the colon. Representative western-blot of NAPE-PLD and β-Actin ( n = 9–10). c Levels of N -acylethanolamines and 2-acylglycerols in small intestinal epithelial cells of ND-fed WT and Napepld ∆IEC mice ( n = 7–9) were determined by using high-performance liquid chromatography-MS using an LTQ Orbitrap mass spectrometer as described in the methods. Dark blue: WT ND mice, light blue: Napepld ∆IEC ND mice. Data are presented as the mean ± s.e.m. *, ** and *** indicate a significant difference versus WT ND (Respectively P

    Article Snippet: The resulting lipid fraction was analysed by high-performance liquid chromatography-MS using an LTQ Orbitrap mass spectrometer (ThermoFisher Scientific) coupled to an Accela HPLC system (ThermoFisher Scientific).

    Techniques: Expressing, Mouse Assay, Western Blot, High Performance Liquid Chromatography, Mass Spectrometry