high performance liquid chromatograph  (Thermo Fisher)


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    Thermo Fisher high performance liquid chromatograph
    High Performance Liquid Chromatograph, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high performance liquid chromatograph/product/Thermo Fisher
    Average 99 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    high performance liquid chromatograph - by Bioz Stars, 2020-07
    99/100 stars

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    Reversed-phase Chromatography:

    Article Title: Fumarate Mediates a Chronic Proliferative Signal in Fumarate Hydratase-Inactivated Cancer Cells by Increasing Transcription and Translation of Ferritin Genes
    Article Snippet: .. In-gel digests were then desalted, fractionated online by reversed-phase chromatography using a Thermo Fisher easy-nLC 1000 liquid chromatography system, and analyzed by tandem mass spectrometry on a Thermo Fisher Q-Exactive mass spectrometer ( , ). .. Database searching was performed by using the MSGF+ search algorithm and a human protein database and considered differential modification of cysteine residues by carbamidomethylation (+57.021464 atomic mass units [amu]), 2-succination (+116.010959 amu), 2-monomethyl-succination (+130.026609 amu), and 2-dimethyl-succination (+144.042259 amu) ( ).

    Liquid Chromatography:

    Article Title: ACP acylation is an acetyl-CoA-dependent modification required for electron transport chain assembly
    Article Snippet: .. Mass spectrometry data were collected using an Orbitrap Fusion Lumos mass spectrometer (Thermo Fischer Scientific) equipped with a Proxeon EASY-nLC 1000 liquid chromatography (LC) system (Thermo Fisher Scientific). .. Peptides were separated on a 100μm inner diameter microcapillary column packed with ~35 cm of Accucore C18 resin (2.6μm, 150 Å, Thermo Fisher Scientific).

    Article Title: Reverse engineering directed gene regulatory networks from transcriptomics and proteomics data of biomining bacterial communities with approximate Bayesian computation and steady-state signalling simulations
    Article Snippet: .. Mass spectrometry for continuous-culture samples was performed by using an EASY-nLC 1000 liquid chromatography (LC) system (Thermo Scientific) and a Q-Exactive HF mass spectrometer (Thermo Scientific), as previously reported [ ]. .. Mass spectra were recorded with Xcalibur software 3.1.66.10 (Thermo Scientific).

    Article Title: Fumarate Mediates a Chronic Proliferative Signal in Fumarate Hydratase-Inactivated Cancer Cells by Increasing Transcription and Translation of Ferritin Genes
    Article Snippet: .. In-gel digests were then desalted, fractionated online by reversed-phase chromatography using a Thermo Fisher easy-nLC 1000 liquid chromatography system, and analyzed by tandem mass spectrometry on a Thermo Fisher Q-Exactive mass spectrometer ( , ). .. Database searching was performed by using the MSGF+ search algorithm and a human protein database and considered differential modification of cysteine residues by carbamidomethylation (+57.021464 atomic mass units [amu]), 2-succination (+116.010959 amu), 2-monomethyl-succination (+130.026609 amu), and 2-dimethyl-succination (+144.042259 amu) ( ).

    Article Title: Quantitative Proteomic Profiling of Tachyplesin I Targets in U251 Gliomaspheres
    Article Snippet: .. After lyophilization in Speedvac, samples were resuspended in 0.1% FA and analyzed by a Q-Exactive orbitrap mass spectrometer (Thermo Fisher Scientific) coupled to an Easy-nLC 1000 (Thermo Fisher Scientific) ultrahigh pressure liquid chromatography (UHPLC). ..

    Mass Spectrometry:

    Article Title: ACP acylation is an acetyl-CoA-dependent modification required for electron transport chain assembly
    Article Snippet: .. Mass spectrometry data were collected using an Orbitrap Fusion Lumos mass spectrometer (Thermo Fischer Scientific) equipped with a Proxeon EASY-nLC 1000 liquid chromatography (LC) system (Thermo Fisher Scientific). .. Peptides were separated on a 100μm inner diameter microcapillary column packed with ~35 cm of Accucore C18 resin (2.6μm, 150 Å, Thermo Fisher Scientific).

    Article Title: Reverse engineering directed gene regulatory networks from transcriptomics and proteomics data of biomining bacterial communities with approximate Bayesian computation and steady-state signalling simulations
    Article Snippet: .. Mass spectrometry for continuous-culture samples was performed by using an EASY-nLC 1000 liquid chromatography (LC) system (Thermo Scientific) and a Q-Exactive HF mass spectrometer (Thermo Scientific), as previously reported [ ]. .. Mass spectra were recorded with Xcalibur software 3.1.66.10 (Thermo Scientific).

    Article Title: Fumarate Mediates a Chronic Proliferative Signal in Fumarate Hydratase-Inactivated Cancer Cells by Increasing Transcription and Translation of Ferritin Genes
    Article Snippet: .. In-gel digests were then desalted, fractionated online by reversed-phase chromatography using a Thermo Fisher easy-nLC 1000 liquid chromatography system, and analyzed by tandem mass spectrometry on a Thermo Fisher Q-Exactive mass spectrometer ( , ). .. Database searching was performed by using the MSGF+ search algorithm and a human protein database and considered differential modification of cysteine residues by carbamidomethylation (+57.021464 atomic mass units [amu]), 2-succination (+116.010959 amu), 2-monomethyl-succination (+130.026609 amu), and 2-dimethyl-succination (+144.042259 amu) ( ).

    Article Title: Functional characterisation of a novel class of in-frame insertion variants of KRAS and HRAS
    Article Snippet: .. Mass spectroscopy After clean up peptides obtained from the trypsin digested precipitates were separated on a 30-cm pico-tip column (50 μm ID, New Objective) packed with 3 μm aquapur gold C-18 material (Dr. Maisch) by applying a gradient (7–80% ACN 0.1% FA, 140 min), delivered by an easy-nLC 1000 system (LC120, Thermo Scientific), and electro-sprayed directly into an LTQ Orbitrap Mass Spectrometer (Velos, Thermo Scientific). .. Raw files were analysed with the MaxQuant software version 1.5.1.0. with oxidation of methionine set as variable and carbamidomethylation of cysteine as fixed modification.

    Article Title: The unfolded protein response affects readthrough of premature termination codons
    Article Snippet: .. Patient samples were analyzed on an EASY-nLC-1000 coupled to the Q-Exactive mass spectrometer (Thermo Fisher Scientific). .. Peptides were separated on a 50-cm C18 column (Dionex) using a 200-min gradient of water/acetonitrile.

    Article Title: Quantitative Proteomic Profiling of Tachyplesin I Targets in U251 Gliomaspheres
    Article Snippet: .. After lyophilization in Speedvac, samples were resuspended in 0.1% FA and analyzed by a Q-Exactive orbitrap mass spectrometer (Thermo Fisher Scientific) coupled to an Easy-nLC 1000 (Thermo Fisher Scientific) ultrahigh pressure liquid chromatography (UHPLC). ..

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    Thermo Fisher high performance liquid chromatography mass spectrometry hplc ms hplc programs
    Category of <t>HPLC-MS-identified</t> <t>glycylglycerins.</t> In human iPSCs, the glycylation of glycerin forms three different kinds of glycylglycerins, including monoglycylglycerins (MGG), diglycylglycerins (DGG) and triglycylglycerin (TGG). All these glycylglycerins carry positive charges in their amino groups at ≤pH 7.0 and can form isoforms as shown in the right bottom corner box.
    High Performance Liquid Chromatography Mass Spectrometry Hplc Ms Hplc Programs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high performance liquid chromatography mass spectrometry hplc ms hplc programs/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high performance liquid chromatography mass spectrometry hplc ms hplc programs - by Bioz Stars, 2020-07
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    88
    Thermo Fisher ultra performance liquid chromatography high resolution mass spectrometry uplc hrms
    Box and whisker plots representing the range of normalized relative intensities for significant metabolic changes in the BBD microbial community after 24 h exposure to 50 µg/L MC-LR. Data are from <t>UPLC-HRMS</t> measurement of triplicate samples. Significance was determined by a fold change > 1.5 and a p value of
    Ultra Performance Liquid Chromatography High Resolution Mass Spectrometry Uplc Hrms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultra performance liquid chromatography high resolution mass spectrometry uplc hrms/product/Thermo Fisher
    Average 88 stars, based on 9 article reviews
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    86
    Thermo Fisher high performance liquid chromatography electrospray ionization ion trap mass spectrometry
    Negative ion HPLC-ESI-IT-MS 2 analysis of hydroethanolic extract of P. torta leaves. Total ion chromatogram (a) ; extracted ion chromatograms for m/z 479 (b) ; m/z 449 (c) ; and m/z 463 (d) . HPLC-ESI-IT-MS 2 , high-performance liquid <t>chromatography–electrospray</t>
    High Performance Liquid Chromatography Electrospray Ionization Ion Trap Mass Spectrometry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high performance liquid chromatography electrospray ionization ion trap mass spectrometry/product/Thermo Fisher
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    86
    Thermo Fisher high performance liquid chromatography ms
    Validation of IEC Napepld deletion. a Napepld mRNA expression in the jejunum, colon, liver and epididymal adipose tissue (EAT) in ND-fed WT and Napepld ∆IEC mice ( n = 8–10). b NAPE-PLD protein levels in the colon. Representative western-blot of NAPE-PLD and β-Actin ( n = 9–10). c Levels of N -acylethanolamines and 2-acylglycerols in small intestinal epithelial cells of ND-fed WT and Napepld ∆IEC mice ( n = 7–9) were determined by using <t>high-performance</t> <t>liquid</t> <t>chromatography-MS</t> using an LTQ Orbitrap mass spectrometer as described in the methods. Dark blue: WT ND mice, light blue: Napepld ∆IEC ND mice. Data are presented as the mean ± s.e.m. *, ** and *** indicate a significant difference versus WT ND (Respectively P
    High Performance Liquid Chromatography Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high performance liquid chromatography ms/product/Thermo Fisher
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
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    Category of HPLC-MS-identified glycylglycerins. In human iPSCs, the glycylation of glycerin forms three different kinds of glycylglycerins, including monoglycylglycerins (MGG), diglycylglycerins (DGG) and triglycylglycerin (TGG). All these glycylglycerins carry positive charges in their amino groups at ≤pH 7.0 and can form isoforms as shown in the right bottom corner box.

    Journal: Nucleic Acids Research

    Article Title: Novel glycylated sugar alcohols protect ESC-specific microRNAs from degradation in iPS cells

    doi: 10.1093/nar/gkw186

    Figure Lengend Snippet: Category of HPLC-MS-identified glycylglycerins. In human iPSCs, the glycylation of glycerin forms three different kinds of glycylglycerins, including monoglycylglycerins (MGG), diglycylglycerins (DGG) and triglycylglycerin (TGG). All these glycylglycerins carry positive charges in their amino groups at ≤pH 7.0 and can form isoforms as shown in the right bottom corner box.

    Article Snippet: Analyses of glycylglycerins using high performance liquid chromatography—mass spectrometry (HPLC-MS) HPLC programs were run by an Ultimate 3000 HPLC machine (Thermo Scientific, Waltham, MA, USA) with a DNAPac PA-100 column (BioLC Semi-Prep 9 × 250 mm) at a flow rate of 3.6 ml/min.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    HPLC detection of glycylglycerin production and glycylglycerin-bound pre-miRNA. ( A ) HPLC analyses showed that production of glycylglycerins can naturally occur in a pure chemical reaction between glycine and glycerin at pH 6.8 in the presence of L-ascorbic acid (vit.C), but not in the same reaction at pH 7.3. ( B ) HPLC peak of DGG/TGG-mixed pre-miR-302a was shifted from 12.23 min at pH 7.3 (unbound) to 1.76 min at pH 6.8 (DGG/TGG-bound), indicating a marked loss of pre-miRNA negative charges after binding with DGG/TGG. After HPLC separation at pH 6.8, the DGG/TGG-bound pre-miR-302a was collected from the 1.7–1.8 min section of HPLC fragments and further used for MS analyses, as shown in Figure 7A . ( C ) Chemical 3D modeling of electro-binding between DGG/TGG and pre-miRNA/siRNA suggested that the long strand structures of 1,3-diglycylglycerin and 1,2,3-triglycylglycerin may fit into the minor grooves of the pre-miRNA/siRNA double helix structures and hence bind to the negatively charged phosphodiester-linkage backbones (green shadow) located in the minor grooves of pre-miRNA/siRNA via the positively charged amino groups of DGG/TGG, so as to form a layer of protective coating for preserving pre-miRNA/siRNA integrity.

    Journal: Nucleic Acids Research

    Article Title: Novel glycylated sugar alcohols protect ESC-specific microRNAs from degradation in iPS cells

    doi: 10.1093/nar/gkw186

    Figure Lengend Snippet: HPLC detection of glycylglycerin production and glycylglycerin-bound pre-miRNA. ( A ) HPLC analyses showed that production of glycylglycerins can naturally occur in a pure chemical reaction between glycine and glycerin at pH 6.8 in the presence of L-ascorbic acid (vit.C), but not in the same reaction at pH 7.3. ( B ) HPLC peak of DGG/TGG-mixed pre-miR-302a was shifted from 12.23 min at pH 7.3 (unbound) to 1.76 min at pH 6.8 (DGG/TGG-bound), indicating a marked loss of pre-miRNA negative charges after binding with DGG/TGG. After HPLC separation at pH 6.8, the DGG/TGG-bound pre-miR-302a was collected from the 1.7–1.8 min section of HPLC fragments and further used for MS analyses, as shown in Figure 7A . ( C ) Chemical 3D modeling of electro-binding between DGG/TGG and pre-miRNA/siRNA suggested that the long strand structures of 1,3-diglycylglycerin and 1,2,3-triglycylglycerin may fit into the minor grooves of the pre-miRNA/siRNA double helix structures and hence bind to the negatively charged phosphodiester-linkage backbones (green shadow) located in the minor grooves of pre-miRNA/siRNA via the positively charged amino groups of DGG/TGG, so as to form a layer of protective coating for preserving pre-miRNA/siRNA integrity.

    Article Snippet: Analyses of glycylglycerins using high performance liquid chromatography—mass spectrometry (HPLC-MS) HPLC programs were run by an Ultimate 3000 HPLC machine (Thermo Scientific, Waltham, MA, USA) with a DNAPac PA-100 column (BioLC Semi-Prep 9 × 250 mm) at a flow rate of 3.6 ml/min.

    Techniques: High Performance Liquid Chromatography, Binding Assay, Mass Spectrometry, Preserving

    Box and whisker plots representing the range of normalized relative intensities for significant metabolic changes in the BBD microbial community after 24 h exposure to 50 µg/L MC-LR. Data are from UPLC-HRMS measurement of triplicate samples. Significance was determined by a fold change > 1.5 and a p value of

    Journal: Life

    Article Title: Ecology and Physiology of the Pathogenic Cyanobacterium Roseofilum reptotaenium

    doi: 10.3390/life4040968

    Figure Lengend Snippet: Box and whisker plots representing the range of normalized relative intensities for significant metabolic changes in the BBD microbial community after 24 h exposure to 50 µg/L MC-LR. Data are from UPLC-HRMS measurement of triplicate samples. Significance was determined by a fold change > 1.5 and a p value of

    Article Snippet: The metabolomes for each sample were then measured via ultra-performance liquid chromatography—high resolution mass spectrometry (UPLC-HRMS) on an Exactive Plus Orbitrap MS (Thermo Fisher, Bremen, Germany) fitted with an UltiMate 3000 UPLC (Dionex, Sunnyvale, CA, USA) using described methods [ ].

    Techniques: Whisker Assay

    Negative ion HPLC-ESI-IT-MS 2 analysis of hydroethanolic extract of P. torta leaves. Total ion chromatogram (a) ; extracted ion chromatograms for m/z 479 (b) ; m/z 449 (c) ; and m/z 463 (d) . HPLC-ESI-IT-MS 2 , high-performance liquid chromatography–electrospray

    Journal: Journal of Medicinal Food

    Article Title: Flavonoid Detection in Hydroethanolic Extract of Pouteria torta (Sapotaceae) Leaves by HPLC-DAD and the Determination of Its Mutagenic Activity

    doi: 10.1089/jmf.2013.0116

    Figure Lengend Snippet: Negative ion HPLC-ESI-IT-MS 2 analysis of hydroethanolic extract of P. torta leaves. Total ion chromatogram (a) ; extracted ion chromatograms for m/z 479 (b) ; m/z 449 (c) ; and m/z 463 (d) . HPLC-ESI-IT-MS 2 , high-performance liquid chromatography–electrospray

    Article Snippet: High-performance liquid chromatography–electrospray ionization-ion trap mass spectrometry (HPLC-ESI-IT-MS ) was performed on a HPLC coupled to a mass spectrometer LCQ Fleet (Thermo Scientific® , Madison, WI, USA) and equipped with a direct insertion deposition flow injection analyzer.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    Validation of IEC Napepld deletion. a Napepld mRNA expression in the jejunum, colon, liver and epididymal adipose tissue (EAT) in ND-fed WT and Napepld ∆IEC mice ( n = 8–10). b NAPE-PLD protein levels in the colon. Representative western-blot of NAPE-PLD and β-Actin ( n = 9–10). c Levels of N -acylethanolamines and 2-acylglycerols in small intestinal epithelial cells of ND-fed WT and Napepld ∆IEC mice ( n = 7–9) were determined by using high-performance liquid chromatography-MS using an LTQ Orbitrap mass spectrometer as described in the methods. Dark blue: WT ND mice, light blue: Napepld ∆IEC ND mice. Data are presented as the mean ± s.e.m. *, ** and *** indicate a significant difference versus WT ND (Respectively P

    Journal: Nature Communications

    Article Title: Intestinal epithelial N-acylphosphatidylethanolamine phospholipase D links dietary fat to metabolic adaptations in obesity and steatosis

    doi: 10.1038/s41467-018-08051-7

    Figure Lengend Snippet: Validation of IEC Napepld deletion. a Napepld mRNA expression in the jejunum, colon, liver and epididymal adipose tissue (EAT) in ND-fed WT and Napepld ∆IEC mice ( n = 8–10). b NAPE-PLD protein levels in the colon. Representative western-blot of NAPE-PLD and β-Actin ( n = 9–10). c Levels of N -acylethanolamines and 2-acylglycerols in small intestinal epithelial cells of ND-fed WT and Napepld ∆IEC mice ( n = 7–9) were determined by using high-performance liquid chromatography-MS using an LTQ Orbitrap mass spectrometer as described in the methods. Dark blue: WT ND mice, light blue: Napepld ∆IEC ND mice. Data are presented as the mean ± s.e.m. *, ** and *** indicate a significant difference versus WT ND (Respectively P

    Article Snippet: The resulting lipid fraction was analysed by high-performance liquid chromatography-MS using an LTQ Orbitrap mass spectrometer (ThermoFisher Scientific) coupled to an Accela HPLC system (ThermoFisher Scientific).

    Techniques: Expressing, Mouse Assay, Western Blot, High Performance Liquid Chromatography, Mass Spectrometry