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Polysciences inc high molecular weight pei
LLO activity of <t>LLO-s-s-PEI</t> monitored by in vitro red blood cell hemolysis assay. <t>LLO-s-s-PEI25</t> was assayed for its ability to lyse red blood cells (RBCs) by monitoring the change in scattering of 590 nm light by 2×10 8 RBCs/ml in a Spex Fluoromax-2
High Molecular Weight Pei, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/high molecular weight pei/product/Polysciences inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
high molecular weight pei - by Bioz Stars, 2021-09
86/100 stars

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1) Product Images from "Enhanced gene delivery using disulfide-crosslinked low molecular weight polyethylenimine with listeriolysin o-polyethylenimine disulfide conjugate"

Article Title: Enhanced gene delivery using disulfide-crosslinked low molecular weight polyethylenimine with listeriolysin o-polyethylenimine disulfide conjugate

Journal:

doi: 10.1016/j.jconrel.2008.07.007

LLO activity of LLO-s-s-PEI monitored by in vitro red blood cell hemolysis assay. LLO-s-s-PEI25 was assayed for its ability to lyse red blood cells (RBCs) by monitoring the change in scattering of 590 nm light by 2×10 8 RBCs/ml in a Spex Fluoromax-2
Figure Legend Snippet: LLO activity of LLO-s-s-PEI monitored by in vitro red blood cell hemolysis assay. LLO-s-s-PEI25 was assayed for its ability to lyse red blood cells (RBCs) by monitoring the change in scattering of 590 nm light by 2×10 8 RBCs/ml in a Spex Fluoromax-2

Techniques Used: Activity Assay, In Vitro, Hemolysis Assay

Transfection with three different forms of PEIs (different molecular weights and different crosslinking: s-s PEI1.8; PEI1.8; PEI25) (a) The effect of disulfide-crosslinking of PEI1.8 on luciferase gene expression in HEK 293 cells at different PEI/pDNA
Figure Legend Snippet: Transfection with three different forms of PEIs (different molecular weights and different crosslinking: s-s PEI1.8; PEI1.8; PEI25) (a) The effect of disulfide-crosslinking of PEI1.8 on luciferase gene expression in HEK 293 cells at different PEI/pDNA

Techniques Used: Transfection, Luciferase, Expressing

The effect of increasing amounts of LLO-s-s-PEI25, incorporated in the PEI/pDNA condensates, on luciferase gene expression in HEK 293 cells. The amount of LLO-s-s-PEI25 incorporated in the PEI/pDNA complex (expressed as w/w % of LLO-s-s-PEI25 relative
Figure Legend Snippet: The effect of increasing amounts of LLO-s-s-PEI25, incorporated in the PEI/pDNA condensates, on luciferase gene expression in HEK 293 cells. The amount of LLO-s-s-PEI25 incorporated in the PEI/pDNA complex (expressed as w/w % of LLO-s-s-PEI25 relative

Techniques Used: Luciferase, Expressing

Related Articles

Transfection:

Article Title: Synthetic gene network restoring endogenous pituitary–thyroid feedback control in experimental Graves’ disease
Article Snippet: .. For the transfection of hMSC-TERT, HEK-293T, HeLa, HT-1080, and CHO-K1, 2 × 105 cells were seeded per well of a six-well plate 24 h before transfection and incubated for 12 h with 400 µL DNA-PEI (polyethylenimine) mixture that was produced by incubating 6 µL PEI for hMSC-TERT and HEK-293T and 12 µL PEI for HT-1080, HeLa, and CHO-K1 (PEI < 20,000 molecular weight; Polysciences; stock solution: 1 mg/mL double-distilled H2 O, pH 7.2) with 3 µg total DNA, vortexing for 5 s, and incubating for 15 min at 22 °C. ..

Article Title: Assessment and optimization of Theileria parva sporozoite full-length p67 antigen expression in mammalian cells
Article Snippet: .. Lentivirus reconstitution and transduction Briefly, HEK 293T cells were transfected in a T175 cm2 flask with 25 μg of transfer vector pEF1α-p67ΔTM-iresGFP, 13 μg of packaging vector p8.74, 10 μg of pseudotyping vector pMD2 and 10 μg of pREV using Polyethylenimine (PEI) transfection reagent (Polysciences, Inc.). ..

Article Title: Heparan Sulfate Organizes Neuronal Synapses through Neurexin Partnerships
Article Snippet: .. For the rest of the recombinant proteins in this study, the protein expression constructs were transfected into HEK293 using PEI “Max” (Polysciences, Inc.). ..

Article Title: Assessment and optimization of Theileria parva sporozoite full-length p67 antigen expression in mammalian cells
Article Snippet: .. When cells were sub-confluent, the culture medium was removed and the cells were transfected with pCMV-p67, pCMV-p67ΔTM, pCMV-p67ΔGlyco, psecE2 [ , ] and pEGFP-C1 using PEI transfection reagent (Polysciences, Inc.). ..

Incubation:

Article Title: Synthetic gene network restoring endogenous pituitary–thyroid feedback control in experimental Graves’ disease
Article Snippet: .. For the transfection of hMSC-TERT, HEK-293T, HeLa, HT-1080, and CHO-K1, 2 × 105 cells were seeded per well of a six-well plate 24 h before transfection and incubated for 12 h with 400 µL DNA-PEI (polyethylenimine) mixture that was produced by incubating 6 µL PEI for hMSC-TERT and HEK-293T and 12 µL PEI for HT-1080, HeLa, and CHO-K1 (PEI < 20,000 molecular weight; Polysciences; stock solution: 1 mg/mL double-distilled H2 O, pH 7.2) with 3 µg total DNA, vortexing for 5 s, and incubating for 15 min at 22 °C. ..

Produced:

Article Title: Synthetic gene network restoring endogenous pituitary–thyroid feedback control in experimental Graves’ disease
Article Snippet: .. For the transfection of hMSC-TERT, HEK-293T, HeLa, HT-1080, and CHO-K1, 2 × 105 cells were seeded per well of a six-well plate 24 h before transfection and incubated for 12 h with 400 µL DNA-PEI (polyethylenimine) mixture that was produced by incubating 6 µL PEI for hMSC-TERT and HEK-293T and 12 µL PEI for HT-1080, HeLa, and CHO-K1 (PEI < 20,000 molecular weight; Polysciences; stock solution: 1 mg/mL double-distilled H2 O, pH 7.2) with 3 µg total DNA, vortexing for 5 s, and incubating for 15 min at 22 °C. ..

Molecular Weight:

Article Title: Synthetic gene network restoring endogenous pituitary–thyroid feedback control in experimental Graves’ disease
Article Snippet: .. For the transfection of hMSC-TERT, HEK-293T, HeLa, HT-1080, and CHO-K1, 2 × 105 cells were seeded per well of a six-well plate 24 h before transfection and incubated for 12 h with 400 µL DNA-PEI (polyethylenimine) mixture that was produced by incubating 6 µL PEI for hMSC-TERT and HEK-293T and 12 µL PEI for HT-1080, HeLa, and CHO-K1 (PEI < 20,000 molecular weight; Polysciences; stock solution: 1 mg/mL double-distilled H2 O, pH 7.2) with 3 µg total DNA, vortexing for 5 s, and incubating for 15 min at 22 °C. ..

Article Title: Enhanced gene delivery using disulfide-crosslinked low molecular weight polyethylenimine with listeriolysin o-polyethylenimine disulfide conjugate
Article Snippet: .. Low molecular weight PEI (branched, average molecular weight 1.8 kDa: PEI1.8) and high molecular weight PEI (branched, average molecular weight 25 kDa: PEI25) were purchased from Polysciences Inc (Warrington, PA). ..

Transduction:

Article Title: Assessment and optimization of Theileria parva sporozoite full-length p67 antigen expression in mammalian cells
Article Snippet: .. Lentivirus reconstitution and transduction Briefly, HEK 293T cells were transfected in a T175 cm2 flask with 25 μg of transfer vector pEF1α-p67ΔTM-iresGFP, 13 μg of packaging vector p8.74, 10 μg of pseudotyping vector pMD2 and 10 μg of pREV using Polyethylenimine (PEI) transfection reagent (Polysciences, Inc.). ..

Plasmid Preparation:

Article Title: Assessment and optimization of Theileria parva sporozoite full-length p67 antigen expression in mammalian cells
Article Snippet: .. Lentivirus reconstitution and transduction Briefly, HEK 293T cells were transfected in a T175 cm2 flask with 25 μg of transfer vector pEF1α-p67ΔTM-iresGFP, 13 μg of packaging vector p8.74, 10 μg of pseudotyping vector pMD2 and 10 μg of pREV using Polyethylenimine (PEI) transfection reagent (Polysciences, Inc.). ..

Recombinant:

Article Title: Heparan Sulfate Organizes Neuronal Synapses through Neurexin Partnerships
Article Snippet: .. For the rest of the recombinant proteins in this study, the protein expression constructs were transfected into HEK293 using PEI “Max” (Polysciences, Inc.). ..

Expressing:

Article Title: Heparan Sulfate Organizes Neuronal Synapses through Neurexin Partnerships
Article Snippet: .. For the rest of the recombinant proteins in this study, the protein expression constructs were transfected into HEK293 using PEI “Max” (Polysciences, Inc.). ..

Construct:

Article Title: Heparan Sulfate Organizes Neuronal Synapses through Neurexin Partnerships
Article Snippet: .. For the rest of the recombinant proteins in this study, the protein expression constructs were transfected into HEK293 using PEI “Max” (Polysciences, Inc.). ..

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    Polysciences inc high molecular weight pei
    LLO activity of <t>LLO-s-s-PEI</t> monitored by in vitro red blood cell hemolysis assay. <t>LLO-s-s-PEI25</t> was assayed for its ability to lyse red blood cells (RBCs) by monitoring the change in scattering of 590 nm light by 2×10 8 RBCs/ml in a Spex Fluoromax-2
    High Molecular Weight Pei, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high molecular weight pei/product/Polysciences inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high molecular weight pei - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    86
    Polysciences inc pei
    LLO activity of <t>LLO-s-s-PEI</t> monitored by in vitro red blood cell hemolysis assay. <t>LLO-s-s-PEI25</t> was assayed for its ability to lyse red blood cells (RBCs) by monitoring the change in scattering of 590 nm light by 2×10 8 RBCs/ml in a Spex Fluoromax-2
    Pei, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pei/product/Polysciences inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pei - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    86
    Polysciences inc high potency polyethylenimine pei
    LLO activity of <t>LLO-s-s-PEI</t> monitored by in vitro red blood cell hemolysis assay. <t>LLO-s-s-PEI25</t> was assayed for its ability to lyse red blood cells (RBCs) by monitoring the change in scattering of 590 nm light by 2×10 8 RBCs/ml in a Spex Fluoromax-2
    High Potency Polyethylenimine Pei, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high potency polyethylenimine pei/product/Polysciences inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high potency polyethylenimine pei - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    LLO activity of LLO-s-s-PEI monitored by in vitro red blood cell hemolysis assay. LLO-s-s-PEI25 was assayed for its ability to lyse red blood cells (RBCs) by monitoring the change in scattering of 590 nm light by 2×10 8 RBCs/ml in a Spex Fluoromax-2

    Journal:

    Article Title: Enhanced gene delivery using disulfide-crosslinked low molecular weight polyethylenimine with listeriolysin o-polyethylenimine disulfide conjugate

    doi: 10.1016/j.jconrel.2008.07.007

    Figure Lengend Snippet: LLO activity of LLO-s-s-PEI monitored by in vitro red blood cell hemolysis assay. LLO-s-s-PEI25 was assayed for its ability to lyse red blood cells (RBCs) by monitoring the change in scattering of 590 nm light by 2×10 8 RBCs/ml in a Spex Fluoromax-2

    Article Snippet: Low molecular weight PEI (branched, average molecular weight 1.8 kDa: PEI1.8) and high molecular weight PEI (branched, average molecular weight 25 kDa: PEI25) were purchased from Polysciences Inc (Warrington, PA).

    Techniques: Activity Assay, In Vitro, Hemolysis Assay

    Transfection with three different forms of PEIs (different molecular weights and different crosslinking: s-s PEI1.8; PEI1.8; PEI25) (a) The effect of disulfide-crosslinking of PEI1.8 on luciferase gene expression in HEK 293 cells at different PEI/pDNA

    Journal:

    Article Title: Enhanced gene delivery using disulfide-crosslinked low molecular weight polyethylenimine with listeriolysin o-polyethylenimine disulfide conjugate

    doi: 10.1016/j.jconrel.2008.07.007

    Figure Lengend Snippet: Transfection with three different forms of PEIs (different molecular weights and different crosslinking: s-s PEI1.8; PEI1.8; PEI25) (a) The effect of disulfide-crosslinking of PEI1.8 on luciferase gene expression in HEK 293 cells at different PEI/pDNA

    Article Snippet: Low molecular weight PEI (branched, average molecular weight 1.8 kDa: PEI1.8) and high molecular weight PEI (branched, average molecular weight 25 kDa: PEI25) were purchased from Polysciences Inc (Warrington, PA).

    Techniques: Transfection, Luciferase, Expressing

    The effect of increasing amounts of LLO-s-s-PEI25, incorporated in the PEI/pDNA condensates, on luciferase gene expression in HEK 293 cells. The amount of LLO-s-s-PEI25 incorporated in the PEI/pDNA complex (expressed as w/w % of LLO-s-s-PEI25 relative

    Journal:

    Article Title: Enhanced gene delivery using disulfide-crosslinked low molecular weight polyethylenimine with listeriolysin o-polyethylenimine disulfide conjugate

    doi: 10.1016/j.jconrel.2008.07.007

    Figure Lengend Snippet: The effect of increasing amounts of LLO-s-s-PEI25, incorporated in the PEI/pDNA condensates, on luciferase gene expression in HEK 293 cells. The amount of LLO-s-s-PEI25 incorporated in the PEI/pDNA complex (expressed as w/w % of LLO-s-s-PEI25 relative

    Article Snippet: Low molecular weight PEI (branched, average molecular weight 1.8 kDa: PEI1.8) and high molecular weight PEI (branched, average molecular weight 25 kDa: PEI25) were purchased from Polysciences Inc (Warrington, PA).

    Techniques: Luciferase, Expressing