high fidelity phushion dna polymerase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher high fidelity phushion dna polymerase
    High Fidelity Phushion Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high fidelity phushion dna polymerase/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    high fidelity phushion dna polymerase - by Bioz Stars, 2020-04
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: ThMYC4E, candidate Blue aleurone 1 gene controlling the associated trait in Triticum aestivum
    Article Snippet: PCR and semi-quantitative PCR PCR amplification was conducted using high-fidelity Phushion DNA polymerase ( Thermo-Fisher Scientific ) in the GeneAmp PCR System 9700 ( Thermo-Fisher Scientific ), which employed the following procedure: 2 min of denaturation at 98°C; 35 cycles of 15 s at 98°C, 30 s at 61°C, and 60 s at 72°C; followed by a final extension of 5 min at 72°C. .. The PCR products were extracted from 1.0% agarose gels using the Tiangen TIAN gel Midi Purification Kit (Tiangen) and were cloned into the pGEM-T Easy Vector plasmid (Promega Corporation, Madison, WI, USA).

    Article Title: Functional MYB transcription factor encoding gene AN2 is associated with anthocyanin biosynthesis in Lycium ruthenicum Murray
    Article Snippet: The high-fidelity Phushion DNA polymerase (Thermo-Fisher Scientific, Shanghai, China)were used in all PCR reactions. .. The PCR products were extracted with the Tiangen TIANgel Midi Purification Kit (Tiangen) from 1.0% agarose gels and were cloned into the pGEM-T Easy Vector plasmid (Promega Corporation, Madison, Wisconsin, USA).

    Amplification:

    Article Title: ThMYC4E, candidate Blue aleurone 1 gene controlling the associated trait in Triticum aestivum
    Article Snippet: .. PCR and semi-quantitative PCR PCR amplification was conducted using high-fidelity Phushion DNA polymerase ( Thermo-Fisher Scientific ) in the GeneAmp PCR System 9700 ( Thermo-Fisher Scientific ), which employed the following procedure: 2 min of denaturation at 98°C; 35 cycles of 15 s at 98°C, 30 s at 61°C, and 60 s at 72°C; followed by a final extension of 5 min at 72°C. .. The PCR products were extracted from 1.0% agarose gels using the Tiangen TIAN gel Midi Purification Kit (Tiangen) and were cloned into the pGEM-T Easy Vector plasmid (Promega Corporation, Madison, WI, USA).

    Synthesized:

    Article Title: Functional MYB transcription factor encoding gene AN2 is associated with anthocyanin biosynthesis in Lycium ruthenicum Murray
    Article Snippet: PCR and semi-quantitative PCR Primers were synthesized by BGI Biological Technology Co., Ltd. .. The high-fidelity Phushion DNA polymerase (Thermo-Fisher Scientific, Shanghai, China)were used in all PCR reactions.

    Purification:

    Article Title: ThMYC4E, candidate Blue aleurone 1 gene controlling the associated trait in Triticum aestivum
    Article Snippet: PCR and semi-quantitative PCR PCR amplification was conducted using high-fidelity Phushion DNA polymerase ( Thermo-Fisher Scientific ) in the GeneAmp PCR System 9700 ( Thermo-Fisher Scientific ), which employed the following procedure: 2 min of denaturation at 98°C; 35 cycles of 15 s at 98°C, 30 s at 61°C, and 60 s at 72°C; followed by a final extension of 5 min at 72°C. .. The PCR products were extracted from 1.0% agarose gels using the Tiangen TIAN gel Midi Purification Kit (Tiangen) and were cloned into the pGEM-T Easy Vector plasmid (Promega Corporation, Madison, WI, USA).

    Article Title: Functional MYB transcription factor encoding gene AN2 is associated with anthocyanin biosynthesis in Lycium ruthenicum Murray
    Article Snippet: The high-fidelity Phushion DNA polymerase (Thermo-Fisher Scientific, Shanghai, China)were used in all PCR reactions. .. The PCR products were extracted with the Tiangen TIANgel Midi Purification Kit (Tiangen) from 1.0% agarose gels and were cloned into the pGEM-T Easy Vector plasmid (Promega Corporation, Madison, Wisconsin, USA).

    Sequencing:

    Article Title: ThMYC4E, candidate Blue aleurone 1 gene controlling the associated trait in Triticum aestivum
    Article Snippet: PCR and semi-quantitative PCR PCR amplification was conducted using high-fidelity Phushion DNA polymerase ( Thermo-Fisher Scientific ) in the GeneAmp PCR System 9700 ( Thermo-Fisher Scientific ), which employed the following procedure: 2 min of denaturation at 98°C; 35 cycles of 15 s at 98°C, 30 s at 61°C, and 60 s at 72°C; followed by a final extension of 5 min at 72°C. .. The coding sequence of ThMYC4E was stored in National Center for Biotechnology Information ( http://www.ncbi.nlm.nih.gov/genbank/ ) under accession number KX914905.

    Polymerase Chain Reaction:

    Article Title: ThMYC4E, candidate Blue aleurone 1 gene controlling the associated trait in Triticum aestivum
    Article Snippet: .. PCR and semi-quantitative PCR PCR amplification was conducted using high-fidelity Phushion DNA polymerase ( Thermo-Fisher Scientific ) in the GeneAmp PCR System 9700 ( Thermo-Fisher Scientific ), which employed the following procedure: 2 min of denaturation at 98°C; 35 cycles of 15 s at 98°C, 30 s at 61°C, and 60 s at 72°C; followed by a final extension of 5 min at 72°C. .. The PCR products were extracted from 1.0% agarose gels using the Tiangen TIAN gel Midi Purification Kit (Tiangen) and were cloned into the pGEM-T Easy Vector plasmid (Promega Corporation, Madison, WI, USA).

    Article Title: Functional MYB transcription factor encoding gene AN2 is associated with anthocyanin biosynthesis in Lycium ruthenicum Murray
    Article Snippet: .. The high-fidelity Phushion DNA polymerase (Thermo-Fisher Scientific, Shanghai, China)were used in all PCR reactions. .. The PCR products were extracted with the Tiangen TIANgel Midi Purification Kit (Tiangen) from 1.0% agarose gels and were cloned into the pGEM-T Easy Vector plasmid (Promega Corporation, Madison, Wisconsin, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Functional MYB transcription factor encoding gene AN2 is associated with anthocyanin biosynthesis in Lycium ruthenicum Murray
    Article Snippet: The high-fidelity Phushion DNA polymerase (Thermo-Fisher Scientific, Shanghai, China)were used in all PCR reactions. .. The semi-quantitative RT-PCR experiments were conducted using previously published methods [ ].

    Transformation Assay:

    Article Title: ThMYC4E, candidate Blue aleurone 1 gene controlling the associated trait in Triticum aestivum
    Article Snippet: PCR and semi-quantitative PCR PCR amplification was conducted using high-fidelity Phushion DNA polymerase ( Thermo-Fisher Scientific ) in the GeneAmp PCR System 9700 ( Thermo-Fisher Scientific ), which employed the following procedure: 2 min of denaturation at 98°C; 35 cycles of 15 s at 98°C, 30 s at 61°C, and 60 s at 72°C; followed by a final extension of 5 min at 72°C. .. The recombinant plasmids were then transformed into Escherichia coli DH5α cells, and six positive clones were sequenced by a commercial company (Huada Gene, Shenzheng, China).

    Article Title: Functional MYB transcription factor encoding gene AN2 is associated with anthocyanin biosynthesis in Lycium ruthenicum Murray
    Article Snippet: The high-fidelity Phushion DNA polymerase (Thermo-Fisher Scientific, Shanghai, China)were used in all PCR reactions. .. The recombinant plasmid was transformed into Escherichia coli DH5α cells, and six positive clones were sequenced in a commercial company (Huada Gene, Shenzheng, China).

    Recombinant:

    Article Title: ThMYC4E, candidate Blue aleurone 1 gene controlling the associated trait in Triticum aestivum
    Article Snippet: PCR and semi-quantitative PCR PCR amplification was conducted using high-fidelity Phushion DNA polymerase ( Thermo-Fisher Scientific ) in the GeneAmp PCR System 9700 ( Thermo-Fisher Scientific ), which employed the following procedure: 2 min of denaturation at 98°C; 35 cycles of 15 s at 98°C, 30 s at 61°C, and 60 s at 72°C; followed by a final extension of 5 min at 72°C. .. The recombinant plasmids were then transformed into Escherichia coli DH5α cells, and six positive clones were sequenced by a commercial company (Huada Gene, Shenzheng, China).

    Article Title: Functional MYB transcription factor encoding gene AN2 is associated with anthocyanin biosynthesis in Lycium ruthenicum Murray
    Article Snippet: The high-fidelity Phushion DNA polymerase (Thermo-Fisher Scientific, Shanghai, China)were used in all PCR reactions. .. The recombinant plasmid was transformed into Escherichia coli DH5α cells, and six positive clones were sequenced in a commercial company (Huada Gene, Shenzheng, China).

    Plasmid Preparation:

    Article Title: ThMYC4E, candidate Blue aleurone 1 gene controlling the associated trait in Triticum aestivum
    Article Snippet: PCR and semi-quantitative PCR PCR amplification was conducted using high-fidelity Phushion DNA polymerase ( Thermo-Fisher Scientific ) in the GeneAmp PCR System 9700 ( Thermo-Fisher Scientific ), which employed the following procedure: 2 min of denaturation at 98°C; 35 cycles of 15 s at 98°C, 30 s at 61°C, and 60 s at 72°C; followed by a final extension of 5 min at 72°C. .. The PCR products were extracted from 1.0% agarose gels using the Tiangen TIAN gel Midi Purification Kit (Tiangen) and were cloned into the pGEM-T Easy Vector plasmid (Promega Corporation, Madison, WI, USA).

    Article Title: Functional MYB transcription factor encoding gene AN2 is associated with anthocyanin biosynthesis in Lycium ruthenicum Murray
    Article Snippet: The high-fidelity Phushion DNA polymerase (Thermo-Fisher Scientific, Shanghai, China)were used in all PCR reactions. .. The PCR products were extracted with the Tiangen TIANgel Midi Purification Kit (Tiangen) from 1.0% agarose gels and were cloned into the pGEM-T Easy Vector plasmid (Promega Corporation, Madison, Wisconsin, USA).

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  • 99
    Thermo Fisher high fidelity dna polymerase enzyme
    Optimization of PCR amplification of the glutamyl-tRNA reductase gene by using various <t>DMSO</t> concentrations (A); Lane 1: 3% (w/v); Lane 2: 5% (w/v); Lane 3: 7% (w/v); Lane 4: 9% (w/v); and Lane 5: 11% (w/v). PCR product after tailoring the ends and purification by gel extraction kit (B). EcoR V cut, dephosphorylated and purified pBBR1MCS2 (C). <t>DNA</t> ladder (SM0331 Fermentas, M) was loaded into the first well.
    High Fidelity Dna Polymerase Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high fidelity dna polymerase enzyme/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    high fidelity dna polymerase enzyme - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    Optimization of PCR amplification of the glutamyl-tRNA reductase gene by using various DMSO concentrations (A); Lane 1: 3% (w/v); Lane 2: 5% (w/v); Lane 3: 7% (w/v); Lane 4: 9% (w/v); and Lane 5: 11% (w/v). PCR product after tailoring the ends and purification by gel extraction kit (B). EcoR V cut, dephosphorylated and purified pBBR1MCS2 (C). DNA ladder (SM0331 Fermentas, M) was loaded into the first well.

    Journal: Biotechnology, Biotechnological Equipment

    Article Title: Heterologous expression of glutamyl-tRNA reductase gene in Rhodobacter sphaeroides O.U.001 to enhance 5-aminolevulinic acid production

    doi: 10.1080/13102818.2014.978170

    Figure Lengend Snippet: Optimization of PCR amplification of the glutamyl-tRNA reductase gene by using various DMSO concentrations (A); Lane 1: 3% (w/v); Lane 2: 5% (w/v); Lane 3: 7% (w/v); Lane 4: 9% (w/v); and Lane 5: 11% (w/v). PCR product after tailoring the ends and purification by gel extraction kit (B). EcoR V cut, dephosphorylated and purified pBBR1MCS2 (C). DNA ladder (SM0331 Fermentas, M) was loaded into the first well.

    Article Snippet: The reaction was performed in the presence of 3%, 5%, 7%, 9% or 11% (w/v) dimethyl sulphoxide (DMSO), using a high-fidelity DNA polymerase enzyme (Phusion, Thermo Scientific) in a total volume of 20 μL.

    Techniques: Polymerase Chain Reaction, Amplification, Purification, Gel Extraction