Structured Review

TaKaRa high fidelity pcr
High Fidelity Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/high fidelity pcr/product/TaKaRa
Average 98 stars, based on 22 article reviews
Price from $9.99 to $1999.99
high fidelity pcr - by Bioz Stars, 2020-01
98/100 stars

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Related Articles

Clone Assay:

Article Title: Allele-specific Differences in Activity of a Novel Cannabinoid Receptor 1 (CNR1) Gene Intronic Enhancer in Hypothalamus, Dorsal Root Ganglia, and Hippocampus *
Article Snippet: .. ECR1(C) was amplified using high fidelity PCR from human placental DNA and cloned into the pTAL-Luc vector (Clontech) that contains a TATA-like promoter (P TAL) region from the herpes simplex virus-thymidine kinase promoter linked to a luciferase reporter ( D ). .. This commercially available plasmid has been widely used to assay gene regulatory sequences in the past and will subsequently be referred to as pLuc.

Article Title: α-Xylosidase plays essential roles in xyloglucan remodelling, maintenance of cell wall integrity, and seed germination in Arabidopsis thaliana
Article Snippet: Transgenic plants The TRG1/XYL1 promoter region and the gene containing upstream and downstream regions were amplified from Ws genomic DNA by high-fidelity PCR with specific primers ( Supplementary Table S2 ) and PrimeSTAR DNA polymerase (Takara Bio Inc.). .. The product was cloned into the Gateway entry vector using the pENTR Directional TOPO Cloning Kit (Invitrogen, K2400-20SP) and transformed One Shot Chemically Competent Escherichia coli (Invitrogen, C4040-03).

Amplification:

Article Title: Sharing of human milk oligosaccharides degradants within bifidobacterial communities in faecal cultures supplemented with Bifidobacterium bifidum
Article Snippet: .. Direct sequencing of PCR products The genes coding for the HMO-degrading enzymes of B . bifidum TMC3108 were amplified in high-fidelity PCR using PrimeSTAR Max (TaKaRa Bio) and genomic DNA as a template. ..

Article Title: Biased assembly of the nuclear pore complex is required for somatic and germline nuclear differentiation in Tetrahymena
Article Snippet: .. DNA plasmid construction cDNAs encoding nucleoporins were amplified by high-fidelity PCR with PrimeSTAR reagent (Takara, Shiga, Japan) from the first-strand cDNAs prepared by using TRIzol reagent (Life Technologies, Tokyo, Japan) according to the manufacturer's protocol. .. The DNAs were amplified using forward and reverse primers with Xho I and Apa I sites, respectively.

Article Title: Allele-specific Differences in Activity of a Novel Cannabinoid Receptor 1 (CNR1) Gene Intronic Enhancer in Hypothalamus, Dorsal Root Ganglia, and Hippocampus *
Article Snippet: .. ECR1(C) was amplified using high fidelity PCR from human placental DNA and cloned into the pTAL-Luc vector (Clontech) that contains a TATA-like promoter (P TAL) region from the herpes simplex virus-thymidine kinase promoter linked to a luciferase reporter ( D ). .. This commercially available plasmid has been widely used to assay gene regulatory sequences in the past and will subsequently be referred to as pLuc.

Article Title: α-Xylosidase plays essential roles in xyloglucan remodelling, maintenance of cell wall integrity, and seed germination in Arabidopsis thaliana
Article Snippet: .. Transgenic plants The TRG1/XYL1 promoter region and the gene containing upstream and downstream regions were amplified from Ws genomic DNA by high-fidelity PCR with specific primers ( Supplementary Table S2 ) and PrimeSTAR DNA polymerase (Takara Bio Inc.). .. The product was cloned into the Gateway entry vector using the pENTR Directional TOPO Cloning Kit (Invitrogen, K2400-20SP) and transformed One Shot Chemically Competent Escherichia coli (Invitrogen, C4040-03).

Sequencing:

Article Title: Sharing of human milk oligosaccharides degradants within bifidobacterial communities in faecal cultures supplemented with Bifidobacterium bifidum
Article Snippet: .. Direct sequencing of PCR products The genes coding for the HMO-degrading enzymes of B . bifidum TMC3108 were amplified in high-fidelity PCR using PrimeSTAR Max (TaKaRa Bio) and genomic DNA as a template. ..

Article Title: Biased assembly of the nuclear pore complex is required for somatic and germline nuclear differentiation in Tetrahymena
Article Snippet: DNA plasmid construction cDNAs encoding nucleoporins were amplified by high-fidelity PCR with PrimeSTAR reagent (Takara, Shiga, Japan) from the first-strand cDNAs prepared by using TRIzol reagent (Life Technologies, Tokyo, Japan) according to the manufacturer's protocol. .. To express mCherry-tagged nucleoporins, we first prepared a pICHF-1 vector by replacing the EGFP-coding sequence of pIGF-1 with an mCherry-coding sequence that was codon-optimized for T. thermophila (prepared by K. Mochizuki).

Article Title: α-Xylosidase plays essential roles in xyloglucan remodelling, maintenance of cell wall integrity, and seed germination in Arabidopsis thaliana
Article Snippet: Transgenic plants The TRG1/XYL1 promoter region and the gene containing upstream and downstream regions were amplified from Ws genomic DNA by high-fidelity PCR with specific primers ( Supplementary Table S2 ) and PrimeSTAR DNA polymerase (Takara Bio Inc.). .. The insertion of the entry plasmids was confirmed by sequencing with primers listed in Supplementary Table S5 .

Transgenic Assay:

Article Title: α-Xylosidase plays essential roles in xyloglucan remodelling, maintenance of cell wall integrity, and seed germination in Arabidopsis thaliana
Article Snippet: .. Transgenic plants The TRG1/XYL1 promoter region and the gene containing upstream and downstream regions were amplified from Ws genomic DNA by high-fidelity PCR with specific primers ( Supplementary Table S2 ) and PrimeSTAR DNA polymerase (Takara Bio Inc.). .. The product was cloned into the Gateway entry vector using the pENTR Directional TOPO Cloning Kit (Invitrogen, K2400-20SP) and transformed One Shot Chemically Competent Escherichia coli (Invitrogen, C4040-03).

Luciferase:

Article Title: Allele-specific Differences in Activity of a Novel Cannabinoid Receptor 1 (CNR1) Gene Intronic Enhancer in Hypothalamus, Dorsal Root Ganglia, and Hippocampus *
Article Snippet: .. ECR1(C) was amplified using high fidelity PCR from human placental DNA and cloned into the pTAL-Luc vector (Clontech) that contains a TATA-like promoter (P TAL) region from the herpes simplex virus-thymidine kinase promoter linked to a luciferase reporter ( D ). .. This commercially available plasmid has been widely used to assay gene regulatory sequences in the past and will subsequently be referred to as pLuc.

Activity Assay:

Article Title: Allele-specific Differences in Activity of a Novel Cannabinoid Receptor 1 (CNR1) Gene Intronic Enhancer in Hypothalamus, Dorsal Root Ganglia, and Hippocampus *
Article Snippet: ECR1(C) Is Active within Primary Hypothalamic Cell Cultures and DRG but Not in Primary Hippocampal Cells Because CNR1 is expressed in many different parts of the brain and peripheral nervous system such as the hypothalamus, hippocampus, and DRG, we explored the hypothesis that the most common allele, ECR1(C), acted as an enhancer of promoter activity within cells derived from specific regions of the brain. .. ECR1(C) was amplified using high fidelity PCR from human placental DNA and cloned into the pTAL-Luc vector (Clontech) that contains a TATA-like promoter (P TAL) region from the herpes simplex virus-thymidine kinase promoter linked to a luciferase reporter ( D ).

Plasmid Preparation:

Article Title: Biased assembly of the nuclear pore complex is required for somatic and germline nuclear differentiation in Tetrahymena
Article Snippet: .. DNA plasmid construction cDNAs encoding nucleoporins were amplified by high-fidelity PCR with PrimeSTAR reagent (Takara, Shiga, Japan) from the first-strand cDNAs prepared by using TRIzol reagent (Life Technologies, Tokyo, Japan) according to the manufacturer's protocol. .. The DNAs were amplified using forward and reverse primers with Xho I and Apa I sites, respectively.

Article Title: Allele-specific Differences in Activity of a Novel Cannabinoid Receptor 1 (CNR1) Gene Intronic Enhancer in Hypothalamus, Dorsal Root Ganglia, and Hippocampus *
Article Snippet: .. ECR1(C) was amplified using high fidelity PCR from human placental DNA and cloned into the pTAL-Luc vector (Clontech) that contains a TATA-like promoter (P TAL) region from the herpes simplex virus-thymidine kinase promoter linked to a luciferase reporter ( D ). .. This commercially available plasmid has been widely used to assay gene regulatory sequences in the past and will subsequently be referred to as pLuc.

Article Title: α-Xylosidase plays essential roles in xyloglucan remodelling, maintenance of cell wall integrity, and seed germination in Arabidopsis thaliana
Article Snippet: Transgenic plants The TRG1/XYL1 promoter region and the gene containing upstream and downstream regions were amplified from Ws genomic DNA by high-fidelity PCR with specific primers ( Supplementary Table S2 ) and PrimeSTAR DNA polymerase (Takara Bio Inc.). .. The product was cloned into the Gateway entry vector using the pENTR Directional TOPO Cloning Kit (Invitrogen, K2400-20SP) and transformed One Shot Chemically Competent Escherichia coli (Invitrogen, C4040-03).

Expressing:

Article Title: Biased assembly of the nuclear pore complex is required for somatic and germline nuclear differentiation in Tetrahymena
Article Snippet: DNA plasmid construction cDNAs encoding nucleoporins were amplified by high-fidelity PCR with PrimeSTAR reagent (Takara, Shiga, Japan) from the first-strand cDNAs prepared by using TRIzol reagent (Life Technologies, Tokyo, Japan) according to the manufacturer's protocol. .. After digestion by Xho I and Apa I, the PCR products were ligated into the respective restriction sites of pIGF-1, a DNA plasmid used for the exogenous expression of GFP-tagged proteins driven by an MTT1 promoter in T. thermophila ( ).

Polymerase Chain Reaction:

Article Title: Sharing of human milk oligosaccharides degradants within bifidobacterial communities in faecal cultures supplemented with Bifidobacterium bifidum
Article Snippet: .. Direct sequencing of PCR products The genes coding for the HMO-degrading enzymes of B . bifidum TMC3108 were amplified in high-fidelity PCR using PrimeSTAR Max (TaKaRa Bio) and genomic DNA as a template. ..

Article Title: Biased assembly of the nuclear pore complex is required for somatic and germline nuclear differentiation in Tetrahymena
Article Snippet: .. DNA plasmid construction cDNAs encoding nucleoporins were amplified by high-fidelity PCR with PrimeSTAR reagent (Takara, Shiga, Japan) from the first-strand cDNAs prepared by using TRIzol reagent (Life Technologies, Tokyo, Japan) according to the manufacturer's protocol. .. The DNAs were amplified using forward and reverse primers with Xho I and Apa I sites, respectively.

Article Title: Allele-specific Differences in Activity of a Novel Cannabinoid Receptor 1 (CNR1) Gene Intronic Enhancer in Hypothalamus, Dorsal Root Ganglia, and Hippocampus *
Article Snippet: .. ECR1(C) was amplified using high fidelity PCR from human placental DNA and cloned into the pTAL-Luc vector (Clontech) that contains a TATA-like promoter (P TAL) region from the herpes simplex virus-thymidine kinase promoter linked to a luciferase reporter ( D ). .. This commercially available plasmid has been widely used to assay gene regulatory sequences in the past and will subsequently be referred to as pLuc.

Article Title: α-Xylosidase plays essential roles in xyloglucan remodelling, maintenance of cell wall integrity, and seed germination in Arabidopsis thaliana
Article Snippet: .. Transgenic plants The TRG1/XYL1 promoter region and the gene containing upstream and downstream regions were amplified from Ws genomic DNA by high-fidelity PCR with specific primers ( Supplementary Table S2 ) and PrimeSTAR DNA polymerase (Takara Bio Inc.). .. The product was cloned into the Gateway entry vector using the pENTR Directional TOPO Cloning Kit (Invitrogen, K2400-20SP) and transformed One Shot Chemically Competent Escherichia coli (Invitrogen, C4040-03).

Transformation Assay:

Article Title: α-Xylosidase plays essential roles in xyloglucan remodelling, maintenance of cell wall integrity, and seed germination in Arabidopsis thaliana
Article Snippet: Transgenic plants The TRG1/XYL1 promoter region and the gene containing upstream and downstream regions were amplified from Ws genomic DNA by high-fidelity PCR with specific primers ( Supplementary Table S2 ) and PrimeSTAR DNA polymerase (Takara Bio Inc.). .. The product was cloned into the Gateway entry vector using the pENTR Directional TOPO Cloning Kit (Invitrogen, K2400-20SP) and transformed One Shot Chemically Competent Escherichia coli (Invitrogen, C4040-03).

Derivative Assay:

Article Title: Allele-specific Differences in Activity of a Novel Cannabinoid Receptor 1 (CNR1) Gene Intronic Enhancer in Hypothalamus, Dorsal Root Ganglia, and Hippocampus *
Article Snippet: ECR1(C) Is Active within Primary Hypothalamic Cell Cultures and DRG but Not in Primary Hippocampal Cells Because CNR1 is expressed in many different parts of the brain and peripheral nervous system such as the hypothalamus, hippocampus, and DRG, we explored the hypothesis that the most common allele, ECR1(C), acted as an enhancer of promoter activity within cells derived from specific regions of the brain. .. ECR1(C) was amplified using high fidelity PCR from human placental DNA and cloned into the pTAL-Luc vector (Clontech) that contains a TATA-like promoter (P TAL) region from the herpes simplex virus-thymidine kinase promoter linked to a luciferase reporter ( D ).

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    TaKaRa la pcr kit
    La Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/la pcr kit/product/TaKaRa
    Average 90 stars, based on 100 article reviews
    Price from $9.99 to $1999.99
    la pcr kit - by Bioz Stars, 2020-01
    90/100 stars
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