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gene exp hif1a mm00468869 m1  (Thermo Fisher)
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Gene Exp Hif1a Mm00468869 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Evaluation of angiogenic, and osteogenic abilities in vitro . (A) Immunofluorescence staining of HUVECs for VEGF. (B) Fluorescence intensity quantification of VEGF. (C and D) The expression levels of angiogenesis-related genes VEGF and <t>HIF-1α</t> in HUVECs. (E) ELISA analysis of VEGF secretion by HUVECs. (F) Western blot analysis of angiogenesis-related proteins in HUVECs. (G and H) Quantitative analysis of Western blot in HUVECs normalized to β-actin. (I) Images of ALP staining on day 7 and 14. (J) Images of ARS staining on day 21 and 28. (K) Quantitative analysis of ALP detected by the ALP assay. (L) Quantitative analysis of calcium nodule elution. (M) Immunofluorescence staining of BMSCs for OPN. (N) Fluorescence intensity quantification of OPN. (O–Q) The expression levels of osteogenic-related genes OPN, COL-I and ALP in BMSCs. (R) Western blot analysis of osteogenesis-related proteins in BMSCs. (S–U) Quantitative analysis of Western blot in BMSCs normalized to β-actin. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
Hif 1α, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Evaluation of angiogenic, and osteogenic abilities in vitro . (A) Immunofluorescence staining of HUVECs for VEGF. (B) Fluorescence intensity quantification of VEGF. (C and D) The expression levels of angiogenesis-related genes VEGF and <t>HIF-1α</t> in HUVECs. (E) ELISA analysis of VEGF secretion by HUVECs. (F) Western blot analysis of angiogenesis-related proteins in HUVECs. (G and H) Quantitative analysis of Western blot in HUVECs normalized to β-actin. (I) Images of ALP staining on day 7 and 14. (J) Images of ARS staining on day 21 and 28. (K) Quantitative analysis of ALP detected by the ALP assay. (L) Quantitative analysis of calcium nodule elution. (M) Immunofluorescence staining of BMSCs for OPN. (N) Fluorescence intensity quantification of OPN. (O–Q) The expression levels of osteogenic-related genes OPN, COL-I and ALP in BMSCs. (R) Western blot analysis of osteogenesis-related proteins in BMSCs. (S–U) Quantitative analysis of Western blot in BMSCs normalized to β-actin. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
Gene Exp Hif1a Hs00153153 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Evaluation of angiogenic, and osteogenic abilities in vitro . (A) Immunofluorescence staining of HUVECs for VEGF. (B) Fluorescence intensity quantification of VEGF. (C and D) The expression levels of angiogenesis-related genes VEGF and <t>HIF-1α</t> in HUVECs. (E) ELISA analysis of VEGF secretion by HUVECs. (F) Western blot analysis of angiogenesis-related proteins in HUVECs. (G and H) Quantitative analysis of Western blot in HUVECs normalized to β-actin. (I) Images of ALP staining on day 7 and 14. (J) Images of ARS staining on day 21 and 28. (K) Quantitative analysis of ALP detected by the ALP assay. (L) Quantitative analysis of calcium nodule elution. (M) Immunofluorescence staining of BMSCs for OPN. (N) Fluorescence intensity quantification of OPN. (O–Q) The expression levels of osteogenic-related genes OPN, COL-I and ALP in BMSCs. (R) Western blot analysis of osteogenesis-related proteins in BMSCs. (S–U) Quantitative analysis of Western blot in BMSCs normalized to β-actin. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
Anti Hif 1α Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Evaluation of angiogenic, and osteogenic abilities in vitro . (A) Immunofluorescence staining of HUVECs for VEGF. (B) Fluorescence intensity quantification of VEGF. (C and D) The expression levels of angiogenesis-related genes VEGF and <t>HIF-1α</t> in HUVECs. (E) ELISA analysis of VEGF secretion by HUVECs. (F) Western blot analysis of angiogenesis-related proteins in HUVECs. (G and H) Quantitative analysis of Western blot in HUVECs normalized to β-actin. (I) Images of ALP staining on day 7 and 14. (J) Images of ARS staining on day 21 and 28. (K) Quantitative analysis of ALP detected by the ALP assay. (L) Quantitative analysis of calcium nodule elution. (M) Immunofluorescence staining of BMSCs for OPN. (N) Fluorescence intensity quantification of OPN. (O–Q) The expression levels of osteogenic-related genes OPN, COL-I and ALP in BMSCs. (R) Western blot analysis of osteogenesis-related proteins in BMSCs. (S–U) Quantitative analysis of Western blot in BMSCs normalized to β-actin. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
Hif1α, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Evaluation of angiogenic, and osteogenic abilities in vitro . (A) Immunofluorescence staining of HUVECs for VEGF. (B) Fluorescence intensity quantification of VEGF. (C and D) The expression levels of angiogenesis-related genes VEGF and HIF-1α in HUVECs. (E) ELISA analysis of VEGF secretion by HUVECs. (F) Western blot analysis of angiogenesis-related proteins in HUVECs. (G and H) Quantitative analysis of Western blot in HUVECs normalized to β-actin. (I) Images of ALP staining on day 7 and 14. (J) Images of ARS staining on day 21 and 28. (K) Quantitative analysis of ALP detected by the ALP assay. (L) Quantitative analysis of calcium nodule elution. (M) Immunofluorescence staining of BMSCs for OPN. (N) Fluorescence intensity quantification of OPN. (O–Q) The expression levels of osteogenic-related genes OPN, COL-I and ALP in BMSCs. (R) Western blot analysis of osteogenesis-related proteins in BMSCs. (S–U) Quantitative analysis of Western blot in BMSCs normalized to β-actin. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Journal: Bioactive Materials

Article Title: Magnesium ion implantation enhances the osseointegration and vascularization of 3D-Printed CoCrMo alloy scaffolds for load-bearing orthopedic applications

doi: 10.1016/j.bioactmat.2025.11.012

Figure Lengend Snippet: Evaluation of angiogenic, and osteogenic abilities in vitro . (A) Immunofluorescence staining of HUVECs for VEGF. (B) Fluorescence intensity quantification of VEGF. (C and D) The expression levels of angiogenesis-related genes VEGF and HIF-1α in HUVECs. (E) ELISA analysis of VEGF secretion by HUVECs. (F) Western blot analysis of angiogenesis-related proteins in HUVECs. (G and H) Quantitative analysis of Western blot in HUVECs normalized to β-actin. (I) Images of ALP staining on day 7 and 14. (J) Images of ARS staining on day 21 and 28. (K) Quantitative analysis of ALP detected by the ALP assay. (L) Quantitative analysis of calcium nodule elution. (M) Immunofluorescence staining of BMSCs for OPN. (N) Fluorescence intensity quantification of OPN. (O–Q) The expression levels of osteogenic-related genes OPN, COL-I and ALP in BMSCs. (R) Western blot analysis of osteogenesis-related proteins in BMSCs. (S–U) Quantitative analysis of Western blot in BMSCs normalized to β-actin. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Western blotting was then performed using the same method as described above, with primary antibody against p-VEGFR2 (Affinity Biosciences, China) and HIF-1α (Proteintech, China).

Techniques: In Vitro, Immunofluorescence, Staining, Fluorescence, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, ALP Assay