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Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI <t>FBS</t> CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show <t>RT-PCR</t> products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p
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1) Product Images from "Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells"

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

Journal: Biological Research

doi: 10.1186/s40659-017-0148-1

Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI FBS CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p
Figure Legend Snippet: Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI FBS CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p

Techniques Used: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p
Figure Legend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

Techniques Used: Transfection, Staining, Cell Counting

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p
Figure Legend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

Techniques Used: Transfection, Staining, Cell Counting

2) Product Images from "Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells"

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

Journal: Biological Research

doi: 10.1186/s40659-017-0148-1

Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI FBS CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p
Figure Legend Snippet: Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI FBS CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p

Techniques Used: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p
Figure Legend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

Techniques Used: Transfection, Staining, Cell Counting

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p
Figure Legend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

Techniques Used: Transfection, Staining, Cell Counting

3) Product Images from "Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells"

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

Journal: Biological Research

doi: 10.1186/s40659-017-0148-1

Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI FBS CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p
Figure Legend Snippet: Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI FBS CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p

Techniques Used: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p
Figure Legend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

Techniques Used: Transfection, Staining, Cell Counting

4) Product Images from "Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells"

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

Journal: Biological Research

doi: 10.1186/s40659-017-0148-1

Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI FBS CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p
Figure Legend Snippet: Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI FBS CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p

Techniques Used: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p
Figure Legend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

Techniques Used: Transfection, Staining, Cell Counting

5) Product Images from "Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells"

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

Journal: Biological Research

doi: 10.1186/s40659-017-0148-1

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p
Figure Legend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

Techniques Used: Transfection, Staining, Cell Counting

6) Product Images from "Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells"

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

Journal: Biological Research

doi: 10.1186/s40659-017-0148-1

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p
Figure Legend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

Techniques Used: Transfection, Staining, Cell Counting

7) Product Images from "Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells"

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

Journal: Biological Research

doi: 10.1186/s40659-017-0148-1

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p
Figure Legend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

Techniques Used: Transfection, Staining, Cell Counting

8) Product Images from "Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells"

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

Journal: Biological Research

doi: 10.1186/s40659-017-0148-1

Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI FBS CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p
Figure Legend Snippet: Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI FBS CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p

Techniques Used: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p
Figure Legend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

Techniques Used: Transfection, Staining, Cell Counting

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p
Figure Legend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

Techniques Used: Transfection, Staining, Cell Counting

9) Product Images from "Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells"

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

Journal: Biological Research

doi: 10.1186/s40659-017-0148-1

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p
Figure Legend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

Techniques Used: Transfection, Staining, Cell Counting

10) Product Images from "Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells"

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

Journal: Biological Research

doi: 10.1186/s40659-017-0148-1

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p
Figure Legend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

Techniques Used: Transfection, Staining, Cell Counting

11) Product Images from "Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells"

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

Journal: Biological Research

doi: 10.1186/s40659-017-0148-1

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p
Figure Legend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

Techniques Used: Transfection, Staining, Cell Counting

12) Product Images from "Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells"

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

Journal: Biological Research

doi: 10.1186/s40659-017-0148-1

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p
Figure Legend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

Techniques Used: Transfection, Staining, Cell Counting

13) Product Images from "Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells"

Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

Journal: Biological Research

doi: 10.1186/s40659-017-0148-1

Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p
Figure Legend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

Techniques Used: Transfection, Staining, Cell Counting

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    Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI <t>FBS</t> CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show <t>RT-PCR</t> products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p
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    Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI FBS CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p

    Journal: Biological Research

    Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

    doi: 10.1186/s40659-017-0148-1

    Figure Lengend Snippet: Effect of siRGS2 on the expression of adipogenic markers during adipogenic differentiation of hMSCs induced by HI FBS CM based adipogenic media. Expression of PPARγ , C/EBPα and LPL were examined at day 1, 3, 5, 7 and 12 post adipogenic treatment initiation in siCON and siRGS2 transfected AD-hMSCs. Graphs represent average expression level of each gene normalized to that of HSP90 and set relative to its normalized expression in siCON transfected cells. Agarose gels show RT-PCR products of examined genes and HSP90 at indicated time points. a Expression of PPARγ in siRGS2 and siCON transfected cells. b Expression of C/EBPα in siRG2 and siCON transfected cells. c Expression of LPL in siRGS2 and siCON transfected cells. Error bars represent variation between independent repeats (n = 2). Expression comparison was made between siCON and siRGS2 or siRGS4 treatment groups at each given time point. *p

    Article Snippet: Expression of RGS2 and RGS4 was examined by RT-PCR in hMSCs cultured in 8 different media treatments, including Hyclone CM, HI-FBS CM, Hyclone CM based DEX media (Hyclone 1.0 µM DEX), HI-FBS CM based DEX media (HI-FBS 1.0 µM DEX), Hyclone CM based AIM media with 1.0 µM DEX (Hyclone 1.0 µM DEX AIM), FBS CM based AIM media with 1 µM DEX (FBS 1.0 µM DEX AIM), HI-FBS CM based AIM media with 1.0 µM DEX (HI-FBS 1.0 µM DEX AIM), and Hyclone CM based OIM media with 1.0 µM DEX (Hyclone 1.0 µM DEX OIM).

    Techniques: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction

    Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

    Journal: Biological Research

    Article Title: Expression regulation and functional analysis of RGS2 and RGS4 in adipogenic and osteogenic differentiation of human mesenchymal stem cells

    doi: 10.1186/s40659-017-0148-1

    Figure Lengend Snippet: Effect of siRGS2 on adipogenic differentiation of hMSCs induced by HI-FBS CM based AIM. Ad-hMSCs were transfected 2 days prior to differentiation induction with HI-FBS CM based AIM media. a Phase contrast images of OilRedO stained wells at day 12 post adipogenic initiation. Lipid droplets were stained red. b OilRedO staining quantification by absorbance reading at 515 nm. c Representative ImagePro area measurement images showing positively stained oil droplets in black and unstained cells in white. d Area measurement quantification of stained oil droplets. e Total cell count based on DAPI nuclear stain. f Adipocytes cell count and percentage of adipocytes. Images and graphs represent the mean quantification of siRGS4 treatment wells set relative to that of siCON wells from a representative experimental set (n = 3). Comparison was made between siCON and siRGS2 treatment groups. *p

    Article Snippet: Expression of RGS2 and RGS4 was examined by RT-PCR in hMSCs cultured in 8 different media treatments, including Hyclone CM, HI-FBS CM, Hyclone CM based DEX media (Hyclone 1.0 µM DEX), HI-FBS CM based DEX media (HI-FBS 1.0 µM DEX), Hyclone CM based AIM media with 1.0 µM DEX (Hyclone 1.0 µM DEX AIM), FBS CM based AIM media with 1 µM DEX (FBS 1.0 µM DEX AIM), HI-FBS CM based AIM media with 1.0 µM DEX (HI-FBS 1.0 µM DEX AIM), and Hyclone CM based OIM media with 1.0 µM DEX (Hyclone 1.0 µM DEX OIM).

    Techniques: Transfection, Staining, Cell Counting