hhai  (New England Biolabs)


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    Structured Review

    New England Biolabs hhai
    (A) The model DNA targets, based on the Mal gene, 64 , 65 are 40 bp or 90 bp in length and consist of 1 (40 bp), 2, 3, 4, or 5 C(*)pGs (90 bp). Location of cytosines in C(*)pG is displayed here by the red circles with nucleotide numbers at the upper strand in the 5′–3′ direction. The methylation-dependent restriction endonucleases <t>HhaI</t> <t>and</t> <t>HpaII</t> recognition sites are indicated by the blue dashed lines. (B) Gel image generated by DNA electrophoresis of Mal3C(*)pG treated with HhaI and HpaII, showing the ladder (L) and HhaI and HpaII-digested Mal3C*pG (1) and Mal3CpG (2). The band at 15 bp in each lane is a marker.
    Hhai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hhai/product/New England Biolabs
    Average 95 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    hhai - by Bioz Stars, 2022-11
    95/100 stars

    Images

    1) Product Images from "Density Control over MBD2 Receptor-Coated Surfaces Provides Superselective Binding of Hypermethylated DNA"

    Article Title: Density Control over MBD2 Receptor-Coated Surfaces Provides Superselective Binding of Hypermethylated DNA

    Journal: ACS Applied Materials & Interfaces

    doi: 10.1021/acsami.2c09641

    (A) The model DNA targets, based on the Mal gene, 64 , 65 are 40 bp or 90 bp in length and consist of 1 (40 bp), 2, 3, 4, or 5 C(*)pGs (90 bp). Location of cytosines in C(*)pG is displayed here by the red circles with nucleotide numbers at the upper strand in the 5′–3′ direction. The methylation-dependent restriction endonucleases HhaI and HpaII recognition sites are indicated by the blue dashed lines. (B) Gel image generated by DNA electrophoresis of Mal3C(*)pG treated with HhaI and HpaII, showing the ladder (L) and HhaI and HpaII-digested Mal3C*pG (1) and Mal3CpG (2). The band at 15 bp in each lane is a marker.
    Figure Legend Snippet: (A) The model DNA targets, based on the Mal gene, 64 , 65 are 40 bp or 90 bp in length and consist of 1 (40 bp), 2, 3, 4, or 5 C(*)pGs (90 bp). Location of cytosines in C(*)pG is displayed here by the red circles with nucleotide numbers at the upper strand in the 5′–3′ direction. The methylation-dependent restriction endonucleases HhaI and HpaII recognition sites are indicated by the blue dashed lines. (B) Gel image generated by DNA electrophoresis of Mal3C(*)pG treated with HhaI and HpaII, showing the ladder (L) and HhaI and HpaII-digested Mal3C*pG (1) and Mal3CpG (2). The band at 15 bp in each lane is a marker.

    Techniques Used: Methylation, Generated, Nucleic Acid Electrophoresis, Marker

    2) Product Images from "Hypermethylation-Mediated Silencing of CIDEA , MAL and PCDH17 Tumour Suppressor Genes in Canine DLBCL: From Multi-Omics Analyses to Mechanistic Studies"

    Article Title: Hypermethylation-Mediated Silencing of CIDEA , MAL and PCDH17 Tumour Suppressor Genes in Canine DLBCL: From Multi-Omics Analyses to Mechanistic Studies

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms23074021

    Luciferase activity of in vitro methylated CiDEA_CpGI1 ( A ), MAL_CpGI1 ( B ), PCDH17_CpGI1 ( C ) and PCDH17_CpGI3 ( D ) plasmids in CF2Th cells. Plasmids containing the CpG sites mostly involved in the regulation of CiDEA , MAL and PCDH17 transcription were subjected to in vitro methylation. SssI, HhaI and HpaII methylation enzymes were used separately or in combination (HhaI + HpaII). Luciferase activity values (mean ± SEM of three independent experiments) are expressed as the percentage of the negative control (CTRL-, the respective unmethylated plasmid) activity, to which an arbitrary value of 100% was assigned. Statistical analysis: ANOVA + Bonferroni post hoc test (*: p < 0.05; ***: p < 0.001).
    Figure Legend Snippet: Luciferase activity of in vitro methylated CiDEA_CpGI1 ( A ), MAL_CpGI1 ( B ), PCDH17_CpGI1 ( C ) and PCDH17_CpGI3 ( D ) plasmids in CF2Th cells. Plasmids containing the CpG sites mostly involved in the regulation of CiDEA , MAL and PCDH17 transcription were subjected to in vitro methylation. SssI, HhaI and HpaII methylation enzymes were used separately or in combination (HhaI + HpaII). Luciferase activity values (mean ± SEM of three independent experiments) are expressed as the percentage of the negative control (CTRL-, the respective unmethylated plasmid) activity, to which an arbitrary value of 100% was assigned. Statistical analysis: ANOVA + Bonferroni post hoc test (*: p < 0.05; ***: p < 0.001).

    Techniques Used: Luciferase, Activity Assay, In Vitro, Methylation, Negative Control, Plasmid Preparation

    Graphical representation of CiDEA_CpGI1, MAL_CpGI1, PCDH17_CpGI1 and PCDH17_CpGI3 regions showing the putative localisation of AP2, MZF1, NF-kB, PAX5 and SP1 TFBSs predicted at SssI, HhaI and HapII methylation sites using MatInspector, Promo3 and TFbind tools. In CiDEA’s graph the position of the TATA box is also shown.
    Figure Legend Snippet: Graphical representation of CiDEA_CpGI1, MAL_CpGI1, PCDH17_CpGI1 and PCDH17_CpGI3 regions showing the putative localisation of AP2, MZF1, NF-kB, PAX5 and SP1 TFBSs predicted at SssI, HhaI and HapII methylation sites using MatInspector, Promo3 and TFbind tools. In CiDEA’s graph the position of the TATA box is also shown.

    Techniques Used: Methylation

    3) Product Images from "Selective amplification of hypermethylated DNA from diverse tumor types via MSRE-PCR"

    Article Title: Selective amplification of hypermethylated DNA from diverse tumor types via MSRE-PCR

    Journal: Oncotarget

    doi: 10.18632/oncotarget.27825

    Methylation sensitive restriction sites in target cDMRs. Five MSREs are considered here: HhaI, HpaII, HpyCH4IV, AciI and BstUI. ( A ) Histogram of the number of MSRE sites in the target regions. ( B ) Scatterplot of the number of MSRE sites versus the number of CpGs in the target regions showing a linear relationship (R 2 = 0.89, p
    Figure Legend Snippet: Methylation sensitive restriction sites in target cDMRs. Five MSREs are considered here: HhaI, HpaII, HpyCH4IV, AciI and BstUI. ( A ) Histogram of the number of MSRE sites in the target regions. ( B ) Scatterplot of the number of MSRE sites versus the number of CpGs in the target regions showing a linear relationship (R 2 = 0.89, p

    Techniques Used: Methylation

    4) Product Images from "5-Aza-2′-deoxycytidine advances the epithelial–mesenchymal transition of breast cancer cells by demethylating Sipa1 promoter-proximal elements"

    Article Title: 5-Aza-2′-deoxycytidine advances the epithelial–mesenchymal transition of breast cancer cells by demethylating Sipa1 promoter-proximal elements

    Journal: Journal of Cell Science

    doi: 10.1242/jcs.236125

    Methylation status of the CpG island stipulates Sipa1 transcription in breast cancer cells. (A) Sipa1 promoter plasmid construction. In the map of the Sipa1 promoter proximal elements, the CpG dinucleotide is indicated by short vertical lines and the CpG island is represented by a rectangle. Three pGL4 plasmids containing different fragments of the possible Sipa1 promoter-proximal elements: SP-1203 contains the entire promoter sequence, SP-895 lacks the CpG island and SP-326 contains the CpG island only. (B) Methylation status of each plasmid examined by digestion with methylation-sensitive restriction enzymes. Lane 1 is a negative control; lanes 2 and 3 represent plasmids treated with M . Sss I without SAM as methylation substrates and digested with methylation-sensitive restriction enzyme HpaI ( Hp ) or HhaI ( Hp ); lanes 4 and 5 represent plasmids treated with M . Sss I and SAM, and digested with HpaI ( Hp ) or HhaI ( Hp ). Blue arrows indicate the digested DNA bands that were sensitive to HpaI or HhaI digestion; red arrows indicate the DNA bands that were more resistant to HpaI or HhaI digestion. (C) Transcriptional activity of the possible Sipa1 promoter-proximal elements with varying degrees of methylation status. Each plasmid was transfected into MDA-MB-231 cells, and the luciferase activity was measured 36 h later. The pGL4-Basic plasmid was used as a negative control. The columns represent the mean of triplicate experiments (*** P
    Figure Legend Snippet: Methylation status of the CpG island stipulates Sipa1 transcription in breast cancer cells. (A) Sipa1 promoter plasmid construction. In the map of the Sipa1 promoter proximal elements, the CpG dinucleotide is indicated by short vertical lines and the CpG island is represented by a rectangle. Three pGL4 plasmids containing different fragments of the possible Sipa1 promoter-proximal elements: SP-1203 contains the entire promoter sequence, SP-895 lacks the CpG island and SP-326 contains the CpG island only. (B) Methylation status of each plasmid examined by digestion with methylation-sensitive restriction enzymes. Lane 1 is a negative control; lanes 2 and 3 represent plasmids treated with M . Sss I without SAM as methylation substrates and digested with methylation-sensitive restriction enzyme HpaI ( Hp ) or HhaI ( Hp ); lanes 4 and 5 represent plasmids treated with M . Sss I and SAM, and digested with HpaI ( Hp ) or HhaI ( Hp ). Blue arrows indicate the digested DNA bands that were sensitive to HpaI or HhaI digestion; red arrows indicate the DNA bands that were more resistant to HpaI or HhaI digestion. (C) Transcriptional activity of the possible Sipa1 promoter-proximal elements with varying degrees of methylation status. Each plasmid was transfected into MDA-MB-231 cells, and the luciferase activity was measured 36 h later. The pGL4-Basic plasmid was used as a negative control. The columns represent the mean of triplicate experiments (*** P

    Techniques Used: Methylation, Plasmid Preparation, Sequencing, Negative Control, Activity Assay, Transfection, Multiple Displacement Amplification, Luciferase

    5) Product Images from "5-Aza-2′-deoxycytidine advances the epithelial–mesenchymal transition of breast cancer cells by demethylating Sipa1 promoter-proximal elements"

    Article Title: 5-Aza-2′-deoxycytidine advances the epithelial–mesenchymal transition of breast cancer cells by demethylating Sipa1 promoter-proximal elements

    Journal: Journal of Cell Science

    doi: 10.1242/jcs.236125

    Methylation status of the CpG island stipulates Sipa1 transcription in breast cancer cells. (A) Sipa1 promoter plasmid construction. In the map of the Sipa1 promoter proximal elements, the CpG dinucleotide is indicated by short vertical lines and the CpG island is represented by a rectangle. Three pGL4 plasmids containing different fragments of the possible Sipa1 promoter-proximal elements: SP-1203 contains the entire promoter sequence, SP-895 lacks the CpG island and SP-326 contains the CpG island only. (B) Methylation status of each plasmid examined by digestion with methylation-sensitive restriction enzymes. Lane 1 is a negative control; lanes 2 and 3 represent plasmids treated with M . Sss I without SAM as methylation substrates and digested with methylation-sensitive restriction enzyme HpaI ( Hp ) or HhaI ( Hp ); lanes 4 and 5 represent plasmids treated with M . Sss I and SAM, and digested with HpaI ( Hp ) or HhaI ( Hp ). Blue arrows indicate the digested DNA bands that were sensitive to HpaI or HhaI digestion; red arrows indicate the DNA bands that were more resistant to HpaI or HhaI digestion. (C) Transcriptional activity of the possible Sipa1 promoter-proximal elements with varying degrees of methylation status. Each plasmid was transfected into MDA-MB-231 cells, and the luciferase activity was measured 36 h later. The pGL4-Basic plasmid was used as a negative control. The columns represent the mean of triplicate experiments (*** P
    Figure Legend Snippet: Methylation status of the CpG island stipulates Sipa1 transcription in breast cancer cells. (A) Sipa1 promoter plasmid construction. In the map of the Sipa1 promoter proximal elements, the CpG dinucleotide is indicated by short vertical lines and the CpG island is represented by a rectangle. Three pGL4 plasmids containing different fragments of the possible Sipa1 promoter-proximal elements: SP-1203 contains the entire promoter sequence, SP-895 lacks the CpG island and SP-326 contains the CpG island only. (B) Methylation status of each plasmid examined by digestion with methylation-sensitive restriction enzymes. Lane 1 is a negative control; lanes 2 and 3 represent plasmids treated with M . Sss I without SAM as methylation substrates and digested with methylation-sensitive restriction enzyme HpaI ( Hp ) or HhaI ( Hp ); lanes 4 and 5 represent plasmids treated with M . Sss I and SAM, and digested with HpaI ( Hp ) or HhaI ( Hp ). Blue arrows indicate the digested DNA bands that were sensitive to HpaI or HhaI digestion; red arrows indicate the DNA bands that were more resistant to HpaI or HhaI digestion. (C) Transcriptional activity of the possible Sipa1 promoter-proximal elements with varying degrees of methylation status. Each plasmid was transfected into MDA-MB-231 cells, and the luciferase activity was measured 36 h later. The pGL4-Basic plasmid was used as a negative control. The columns represent the mean of triplicate experiments (*** P

    Techniques Used: Methylation, Plasmid Preparation, Sequencing, Negative Control, Activity Assay, Transfection, Multiple Displacement Amplification, Luciferase

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    New England Biolabs concentrated hhai enzyme
    Concentrated Hhai Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/concentrated hhai enzyme/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    concentrated hhai enzyme - by Bioz Stars, 2022-11
    95/100 stars
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