hha i  (New England Biolabs)


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    Structured Review

    New England Biolabs hha i
    Hha I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hha i/product/New England Biolabs
    Average 93 stars, based on 84 article reviews
    Price from $9.99 to $1999.99
    hha i - by Bioz Stars, 2020-05
    93/100 stars

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    Polymerase Chain Reaction:

    Article Title: Aerobic degradation of dichlorinated dibenzo-p-dioxin and dichlorinated dibenzofuran by bacteria strains obtained from tropical contaminated soil.
    Article Snippet: .. Bacterial diversity and aerobic catabolic competence of dioxin-degrading bacterial strains isolated from a polluted soil in the tropics were explored. .. Bacterial diversity and aerobic catabolic competence of dioxin-degrading bacterial strains isolated from a polluted soil in the tropics were explored.

    Purification:

    Article Title: Aerobic degradation of dichlorinated dibenzo-p-dioxin and dichlorinated dibenzofuran by bacteria strains obtained from tropical contaminated soil.
    Article Snippet: .. Bacterial diversity and aerobic catabolic competence of dioxin-degrading bacterial strains isolated from a polluted soil in the tropics were explored. .. Bacterial diversity and aerobic catabolic competence of dioxin-degrading bacterial strains isolated from a polluted soil in the tropics were explored.

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    New England Biolabs hha i
    PCR-RFLP of the gltA gene of tick samples. Assays were performed by digestion with <t>Taq</t> I, Hha I, and Mse I. Lane M, 100-bp molecular size ladder; lanes 1, 11, and 21, tick 22; lanes 2, 12, and 22, tick 70; lanes 3, 13, and 23, tick 75; lanes 4, 14, and 24, tick 81; lanes 5, 15, and 25, tick 12; lanes 6, 16, and 26, tick 143; lanes 7, 17, and 27, B. henselae ; lanes 8, 18, and 28, B. vinsonii subsp. berkhoffii ; lanes 9, 19, and 29, Bartonella strain cattle-1; lanes 10, 20, and 30, B. quintana .
    Hha I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hha i/product/New England Biolabs
    Average 96 stars, based on 84 article reviews
    Price from $9.99 to $1999.99
    hha i - by Bioz Stars, 2020-05
    96/100 stars
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    PCR-RFLP of the gltA gene of tick samples. Assays were performed by digestion with Taq I, Hha I, and Mse I. Lane M, 100-bp molecular size ladder; lanes 1, 11, and 21, tick 22; lanes 2, 12, and 22, tick 70; lanes 3, 13, and 23, tick 75; lanes 4, 14, and 24, tick 81; lanes 5, 15, and 25, tick 12; lanes 6, 16, and 26, tick 143; lanes 7, 17, and 27, B. henselae ; lanes 8, 18, and 28, B. vinsonii subsp. berkhoffii ; lanes 9, 19, and 29, Bartonella strain cattle-1; lanes 10, 20, and 30, B. quintana .

    Journal: Journal of Clinical Microbiology

    Article Title: Molecular Evidence of Bartonella spp. in Questing Adult Ixodes pacificus Ticks in California

    doi: 10.1128/JCM.39.4.1221-1226.2001

    Figure Lengend Snippet: PCR-RFLP of the gltA gene of tick samples. Assays were performed by digestion with Taq I, Hha I, and Mse I. Lane M, 100-bp molecular size ladder; lanes 1, 11, and 21, tick 22; lanes 2, 12, and 22, tick 70; lanes 3, 13, and 23, tick 75; lanes 4, 14, and 24, tick 81; lanes 5, 15, and 25, tick 12; lanes 6, 16, and 26, tick 143; lanes 7, 17, and 27, B. henselae ; lanes 8, 18, and 28, B. vinsonii subsp. berkhoffii ; lanes 9, 19, and 29, Bartonella strain cattle-1; lanes 10, 20, and 30, B. quintana .

    Article Snippet: Taq I (Promega, Madison, Wis.), Hha I (New England Biolabs, Beverly, Mass.), and Mse I (New England Biolabs) restriction endonucleases were used for PCR-RFLP analysis of the gltA gene.

    Techniques: Polymerase Chain Reaction

    Agarose gel electrophoresis of KS7-KS8 PCR products digested with Fsp I (A) or Hha I (B) and of PCR amplification products with primers Stx1c-1 and Stx1c-2 (C). M, molecular weight marker (1-kb DNA ladder; Gibco BRL, Eggenstein, Germany). In lanes 1 to 12, the following STEC strains (genotypes, serotypes, and origins, if not human, in parentheses) are shown: 1, 808/97 ( stx 1c + stx 2d ; ONT:H8); 2, 3115/97 ( stx 1c + stx 2d ; O128:H2); 3, 521/99 ( stx 1c + stx 2d ; O rough :H19); 4, 4756/98 ( stx 1c + stx 2d ; O70:H − ); 5, 273/00 ( stx 1c + stx 2d ; O128:H − ; sheep); 6, 295/00 ( stx 1c + stx 2d ; O128:H − ; sheep); 7, EDL 933 ( stx 1 + stx 2 ; O157:H7); 8, 2544/00 ( stx 1 ; O145:H − ); 9, 3385/00 ( stx 1 + stx 2 ; O111:H − ); 10, 4424/99 ( stx 1 + stx 2 ; O157:H7); 11, 2049/98 ( stx 1 + stx 2c ; O157:H − ); 12, 2050/98 ( stx 1 + stx 2 + stx 2c ; O157:H − ).

    Journal: Journal of Clinical Microbiology

    Article Title: Identification, Characterization, and Distribution of a Shiga Toxin 1 Gene Variant (stx1c) in Escherichia coli Strains Isolated from Humans

    doi: 10.1128/JCM.40.4.1441-1446.2002

    Figure Lengend Snippet: Agarose gel electrophoresis of KS7-KS8 PCR products digested with Fsp I (A) or Hha I (B) and of PCR amplification products with primers Stx1c-1 and Stx1c-2 (C). M, molecular weight marker (1-kb DNA ladder; Gibco BRL, Eggenstein, Germany). In lanes 1 to 12, the following STEC strains (genotypes, serotypes, and origins, if not human, in parentheses) are shown: 1, 808/97 ( stx 1c + stx 2d ; ONT:H8); 2, 3115/97 ( stx 1c + stx 2d ; O128:H2); 3, 521/99 ( stx 1c + stx 2d ; O rough :H19); 4, 4756/98 ( stx 1c + stx 2d ; O70:H − ); 5, 273/00 ( stx 1c + stx 2d ; O128:H − ; sheep); 6, 295/00 ( stx 1c + stx 2d ; O128:H − ; sheep); 7, EDL 933 ( stx 1 + stx 2 ; O157:H7); 8, 2544/00 ( stx 1 ; O145:H − ); 9, 3385/00 ( stx 1 + stx 2 ; O111:H − ); 10, 4424/99 ( stx 1 + stx 2 ; O157:H7); 11, 2049/98 ( stx 1 + stx 2c ; O157:H − ); 12, 2050/98 ( stx 1 + stx 2 + stx 2c ; O157:H − ).

    Article Snippet: The stxB 1 gene was amplified with primers KS7-KS8 (Table ), and 12 μl of each PCR product was digested with restriction endonuclease Fsp I or Hha I (New England BioLabs GmbH, Frankfurt, Germany), as recommended by the manufacturer.

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification, Molecular Weight, Marker

    Modulation of demethylation by the IPTG concentration. The 293/ElacI cells were treated with a final IPTG concentration of 5 mM for 4 h before transfection with Hha I-methylated pOLucOriP. The transfected cells were divided among several plates and treated with lower concentrations of IPTG. The fraction of plasmids becoming demethylated in the lacO sites was analyzed as described previously. A linear and inversely correlated relationship between the IPTG concentration and demethylation of lacO was observed.

    Journal: Molecular and Cellular Biology

    Article Title: Modulation of DNA Binding Protein Affinity Directly Affects Target Site Demethylation

    doi:

    Figure Lengend Snippet: Modulation of demethylation by the IPTG concentration. The 293/ElacI cells were treated with a final IPTG concentration of 5 mM for 4 h before transfection with Hha I-methylated pOLucOriP. The transfected cells were divided among several plates and treated with lower concentrations of IPTG. The fraction of plasmids becoming demethylated in the lacO sites was analyzed as described previously. A linear and inversely correlated relationship between the IPTG concentration and demethylation of lacO was observed.

    Article Snippet: DNA was methylated with Hha I- or Sss I-methylase overnight under conditions recommended by the manufacturer (New England Biolabs).

    Techniques: Concentration Assay, Transfection, Methylation

    Demethylation of lacO sites. (A) Illustration of the SV40 intron harboring the three lacO sites and the sizes of Hin dIII and Hha I restriction fragments in the region. (B) Southern blot of Hin dIII- Hha I-double-digested plasmid DNA harvested 15 days after transfection into cell lines with (+ lacI , 293/ElacI) and without (− lacI , 293/EBNA1) lacI expression. The unmethylated plasmid can be digested to completion by Hin dIII and Hha I; therefore, a 338-bp band is detected. The Hha I- or Sss I-methylated plasmids remain methylated at the three Hha I sites within lacO when LacI is absent, so that a 467-bp band is detected. In contrast, these Hha I sites are demethylated when LacI is present, and they are digestable by Hha I to give rise to the 338-bp band. (C) Lack of demethylation in the vector backbone. There is no detectable difference in the Hha I-digested vector backbone with or without lacI .

    Journal: Molecular and Cellular Biology

    Article Title: Modulation of DNA Binding Protein Affinity Directly Affects Target Site Demethylation

    doi:

    Figure Lengend Snippet: Demethylation of lacO sites. (A) Illustration of the SV40 intron harboring the three lacO sites and the sizes of Hin dIII and Hha I restriction fragments in the region. (B) Southern blot of Hin dIII- Hha I-double-digested plasmid DNA harvested 15 days after transfection into cell lines with (+ lacI , 293/ElacI) and without (− lacI , 293/EBNA1) lacI expression. The unmethylated plasmid can be digested to completion by Hin dIII and Hha I; therefore, a 338-bp band is detected. The Hha I- or Sss I-methylated plasmids remain methylated at the three Hha I sites within lacO when LacI is absent, so that a 467-bp band is detected. In contrast, these Hha I sites are demethylated when LacI is present, and they are digestable by Hha I to give rise to the 338-bp band. (C) Lack of demethylation in the vector backbone. There is no detectable difference in the Hha I-digested vector backbone with or without lacI .

    Article Snippet: DNA was methylated with Hha I- or Sss I-methylase overnight under conditions recommended by the manufacturer (New England Biolabs).

    Techniques: Southern Blot, Plasmid Preparation, Transfection, Expressing, Methylation

    The presence of a high concentration of IPTG can prevent lacO demethylation. The 293/ElacI cells were treated with a final IPTG concentration of 5 mM beginning 4 h before transfection. The cells were divided evenly between two plates 3 days after transfection. The cells in one plate were treated with 10 μM IPTG, and the cells in the other plate were not treated with IPTG. Plasmid DNA was harvested for probing multiple times during a 19-day interval for Southern blot analysis using the 467-bp Hin dIII fragment as a probe. When IPTG was present, the Hha I-methylated plasmid remained methylated at the Hha I sites within the lacO sequence at 19 days after transfection (lanes 1 and 2). In contrast, after IPTG was withdrawn from the tissue culture medium 15 days after transfection, demethylation within the lacO sites could be detected 4 days later (lanes 3 and 4).

    Journal: Molecular and Cellular Biology

    Article Title: Modulation of DNA Binding Protein Affinity Directly Affects Target Site Demethylation

    doi:

    Figure Lengend Snippet: The presence of a high concentration of IPTG can prevent lacO demethylation. The 293/ElacI cells were treated with a final IPTG concentration of 5 mM beginning 4 h before transfection. The cells were divided evenly between two plates 3 days after transfection. The cells in one plate were treated with 10 μM IPTG, and the cells in the other plate were not treated with IPTG. Plasmid DNA was harvested for probing multiple times during a 19-day interval for Southern blot analysis using the 467-bp Hin dIII fragment as a probe. When IPTG was present, the Hha I-methylated plasmid remained methylated at the Hha I sites within the lacO sequence at 19 days after transfection (lanes 1 and 2). In contrast, after IPTG was withdrawn from the tissue culture medium 15 days after transfection, demethylation within the lacO sites could be detected 4 days later (lanes 3 and 4).

    Article Snippet: DNA was methylated with Hha I- or Sss I-methylase overnight under conditions recommended by the manufacturer (New England Biolabs).

    Techniques: Concentration Assay, Transfection, Plasmid Preparation, Southern Blot, Methylation, Sequencing

    CAPS procedure for the detection of a thymine to cysteine change (C2088R mutation) at nucleotide position 6262 in Lolium spp. The Hha I digested fragment (126 bp) correspond to the resistant R2088 allele and the Hha 1 undigested fragment (161 bp) correspond to the C2088 allele. Heterozygous plants display both the 126 bp and 161 resistant and sensitive alleles respectively. Lanes 1 and 10: DNA ladder, lanes 2, 3 and 4: homozygous mutant RR2088, lanes 4, 5, 6: heterozygous CR2088 plants, lanes 7, 8, 9: homozygous wild CC2088 plants.

    Journal: PLoS ONE

    Article Title: Broad Resistance to ACCase Inhibiting Herbicides in a Ryegrass Population Is Due Only to a Cysteine to Arginine Mutation in the Target Enzyme

    doi: 10.1371/journal.pone.0039759

    Figure Lengend Snippet: CAPS procedure for the detection of a thymine to cysteine change (C2088R mutation) at nucleotide position 6262 in Lolium spp. The Hha I digested fragment (126 bp) correspond to the resistant R2088 allele and the Hha 1 undigested fragment (161 bp) correspond to the C2088 allele. Heterozygous plants display both the 126 bp and 161 resistant and sensitive alleles respectively. Lanes 1 and 10: DNA ladder, lanes 2, 3 and 4: homozygous mutant RR2088, lanes 4, 5, 6: heterozygous CR2088 plants, lanes 7, 8, 9: homozygous wild CC2088 plants.

    Article Snippet: Two µl aliquots of neat PCR products were digested with 2 units of Hha I (New England Biolabs, Hertfordshire, UK) in a total volume of 20 µL according to the manufacturer's recommendations and analysed on 2% agarose/1 X TBE buffer gels containing 0.5 µg mL−1 ethidium bromide.

    Techniques: Mutagenesis