Journal: International Dental Journal

Article Title: Developing a Silver Nanocomposite With Quorum-Quenching Enzyme Ag-Est816 to Prevent Periodontitis

doi: 10.1016/j.identj.2026.109481

Figure Lengend Snippet: CCK-8 value of HGFs of Ag-Est816 and PBS at Day 1, 3 and 7. Ag-Est816, nanocomposite of silver nanoparticles and N -acyl-homoserine lactone-lactonase Est816; CCK-8, cell counting Kit-8; HGFs, human gingival fibroblasts; PBS, phosphate-buffered saline (control), ns, P > .05).

Article Snippet: The Est816 was prepared and purified according to our established protocols, and its molecular mass was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis., , Human gingival fibroblasts (HGFs) (HGF-1, ATCC CRL-2014) were used in the following experiments.

Techniques: CCK-8 Assay, Cell Counting, Saline, Control

Journal: International Dental Journal

Article Title: Developing a Silver Nanocomposite With Quorum-Quenching Enzyme Ag-Est816 to Prevent Periodontitis

doi: 10.1016/j.identj.2026.109481

Figure Lengend Snippet: Cytocompatibility of HGFs treated with Ag-Est816 nanocomposite and PBS (control) at Day 1, 3 and 7. Ag-Est816, nanocomposite of silver nanoparticles and N -acyl-homoserine lactone-lactonase Est816; PBS, Phosphate-buffered Saline (Control). Immunofluorescence staining: F‑actin labelled the cytoskeleton in red, DAPI labelled nuclei in blue.

Article Snippet: The Est816 was prepared and purified according to our established protocols, and its molecular mass was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis., , Human gingival fibroblasts (HGFs) (HGF-1, ATCC CRL-2014) were used in the following experiments.

Techniques: Control, Saline, Immunofluorescence, Staining

Journal: Bioactive Materials

Article Title: Mesenchymal stromal cells-loaded 3D radially aligned composite scaffold with potentiated paracrine signaling for sequential bone regeneration

doi: 10.1016/j.bioactmat.2026.02.059

Figure Lengend Snippet: Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.

Article Snippet: ELISA kits for PGE2 (Cat. No. E-EL-0034), TGF-β (Cat. No. E-EL-0162), VEGF (Cat. No. E-EL-R2603), and HGF (Cat. No. E-EL-R0496) were purchased from Elabscience (Wuhan, China).

Techniques: Confocal Microscopy, Fluorescence, Staining, Cell Culture, Expressing

Journal: RSC Advances

Article Title: Genipin-crosslinked CeO 2 -incorporating Janus patch for oral ulcer treatment

doi: 10.1039/d6ra00275g

Figure Lengend Snippet: (a) Experimental setup to evaluate the cytotoxicity of individual DL-JPatch components. (b) Comparison of fluorescence images showing human gingival fibroblasts (HGF-1) labeled with Calcein AM (live, green) or Propidium iodide (dead, red) after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). (c) Quantification of the relative proportions of live and dead cell populations. ( n = 5–7 per group, ns: no significance, one-way ANOVA test) (d) cell viability measured by absorbance at 450 nm using CCK-8 assay after 24 h of co-culture with hydrogel patches (blank patch, CHI-G/TA, CHI-G/CeO 2 /Gp). ( n = 3, ns: no significance, one-way ANOVA test) (e) Relative body weight changes over 21 days. Red arrows and dotted lines indicate the days of repetitive patch application (days 1, 2, 7, and 14). ( n = 3, ns: no significance, two-way ANOVA test). (f) Representative H&E-stained images of each organs isolated 7 days after final application. All data are means ± s.d.

Article Snippet: Human gingival fibroblasts (HGF-1) were purchased from ATCC (Manassas, USA).

Techniques: Comparison, Fluorescence, Labeling, Co-Culture Assay, CCK-8 Assay, Staining, Isolation