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hgf  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology hgf
    Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine <t>factors</t> <t>(TGF-β,</t> PGE2, VEGF, <t>HGF,</t> and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.
    Hgf, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hgf/Rat+HGF+(Hepatocyte+Growth+Factor)+ELISA+Kit/pmc12992994-233-16-23
    Average 94 stars, based on 6 article reviews
    hgf - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "Mesenchymal stromal cells-loaded 3D radially aligned composite scaffold with potentiated paracrine signaling for sequential bone regeneration"

    Article Title: Mesenchymal stromal cells-loaded 3D radially aligned composite scaffold with potentiated paracrine signaling for sequential bone regeneration

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.02.059

    Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.
    Figure Legend Snippet: Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.

    Techniques Used: Confocal Microscopy, Fluorescence, Staining, Cell Culture, Expressing

    Related Articles

    other:

    Article Title: Mesenchymal stromal cells-loaded 3D radially aligned composite scaffold with potentiated paracrine signaling for sequential bone regeneration
    Article Snippet: HiScript III RT SuperMix for qPCR, FastPure Cell/Tissue Total RNA Isolation Kit V2, and Taq Pro Universal SYBR qPCR Master Mix were from Vazyme (Nanjing, China).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Anti‐inflammatory properties of conditioned medium from human Wharton’s jelly mesenchymal stem cells
    Article Snippet: .. VEGFα, EGFβ, IL10, and HGF levels in the CM hWJMSCs were assessed using the ELISA kit VEGF α (Elabscience, EELH0111), EGFβ (Elabscience, E ELH0059), IL10 (Elabscience, EELH6154), and HGF (Elabscience, EELH0084). ..

    Article Title: Correlations between Salivary Immuno-Biochemical Markers and HbA1c in Type 2 Diabetes Subjects before and after Dental Extraction
    Article Snippet: .. A number of parameters were determined in saliva, using the ELISA immunenzymatic method: OPG (MyBioSource, San Diego, CA, United States, # MBS175881), RANKL (MyBioSource, San Diego, CA, United States, # MBS268235), HGF (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0084), TNF-α (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0109), MMP-9 (Abcam, CA, United Kingdom, ab246539), IL-18 (Abcam, Cambridge, United Kingdom, ab215539), and the spectrophotometric method determined TAC (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey) and TOS (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey), according to the method described by Erel in 2005 [ ]. ..

    Article Title: Mesenchymal stromal cells-loaded 3D radially aligned composite scaffold with potentiated paracrine signaling for sequential bone regeneration
    Article Snippet: HiScript III RT SuperMix for qPCR, FastPure® Cell/Tissue Total RNA Isolation Kit V2, and Taq Pro Universal SYBR qPCR Master Mix were from Vazyme (Nanjing, China). .. ELISA kits for PGE2 (Cat. No. E-EL-0034), TGF-β (Cat. No. E-EL-0162), VEGF (Cat. No. E-EL-R2603), and HGF (Cat. No. E-EL-R0496) were purchased from Elabscience (Wuhan, China). .. Protein loading buffer, total protein extraction kit, and BCA protein concentration assay kit were purchased from Solarbio (Beijing, China).

    REL Assay:

    Article Title: Correlations between Salivary Immuno-Biochemical Markers and HbA1c in Type 2 Diabetes Subjects before and after Dental Extraction
    Article Snippet: .. A number of parameters were determined in saliva, using the ELISA immunenzymatic method: OPG (MyBioSource, San Diego, CA, United States, # MBS175881), RANKL (MyBioSource, San Diego, CA, United States, # MBS268235), HGF (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0084), TNF-α (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0109), MMP-9 (Abcam, CA, United Kingdom, ab246539), IL-18 (Abcam, Cambridge, United Kingdom, ab215539), and the spectrophotometric method determined TAC (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey) and TOS (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey), according to the method described by Erel in 2005 [ ]. ..



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    Elabscience Biotechnology hgf
    Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine <t>factors</t> <t>(TGF-β,</t> PGE2, VEGF, <t>HGF,</t> and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.
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    Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine <t>factors</t> <t>(TGF-β,</t> PGE2, VEGF, <t>HGF,</t> and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.
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    Image Search Results


    Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.

    Journal: Bioactive Materials

    Article Title: Mesenchymal stromal cells-loaded 3D radially aligned composite scaffold with potentiated paracrine signaling for sequential bone regeneration

    doi: 10.1016/j.bioactmat.2026.02.059

    Figure Lengend Snippet: Temporal analysis of the BMSC paracrine profile on different scaffolds. (A) Confocal microscopy images from Live/Dead fluorescence staining of BMSCs encapsulated within the PCL/HAp-GelMA/BMSCs scaffold after 1, 3, 5, and 14 d of 3D culture (live cells, green; dead cells, red). (B) The concentrations of key paracrine factors (TGF-β, PGE2, VEGF, HGF, and BMP-2) from BMSCs cultured in different scaffolds, quantified from culture supernatants at day 3 and day 7. (C) Corresponding relative mRNA expression levels of TGFB1, PTGS2, VEGFA, HGF, and BMP-2 in BMSCs at day 3 and day 7, as determined by qPCR analysis. Data are presented as mean ± SD (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns: not significant.

    Article Snippet: ELISA kits for PGE2 (Cat. No. E-EL-0034), TGF-β (Cat. No. E-EL-0162), VEGF (Cat. No. E-EL-R2603), and HGF (Cat. No. E-EL-R0496) were purchased from Elabscience (Wuhan, China).

    Techniques: Confocal Microscopy, Fluorescence, Staining, Cell Culture, Expressing