irradiated human foreskin fibroblasts irr hff  (Thermo Fisher)


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    Thermo Fisher irradiated human foreskin fibroblasts irr hff
    Irradiated Human Foreskin Fibroblasts Irr Hff, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hff 1 cells  (ATCC)


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    ATCC hff 1 cells
    Hff 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hff 1 cells  (ATCC)


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    ATCC hff 1 cells
    Hff 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MatTek hff cells
    Hff Cells, supplied by MatTek, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human foreskin fibroblasts hff  (ATCC)


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    ATCC human foreskin fibroblasts hff
    Human Foreskin Fibroblasts Hff, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hff 1 atcc  (ATCC)


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    ATCC hff 1 atcc
    Hff 1 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Spectrum Labs hollow fiber filtration hff
    Hollow Fiber Filtration Hff, supplied by Spectrum Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    confluent hff cells  (Thermo Fisher)


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    Thermo Fisher confluent hff cells
    (a) Structure of Bay 11-7082 and Bay 11-7085 from PubChem ( https://pubchem.ncbi.nlm.nih.gov ); (b) Bay 11-7082 inhibits interactions between apicomplexan IMPα and NLS as shown by AlphaScreen. AlphaScreen technology was used to determine the IC 50 for inhibition by Bay 11-7082 of IMPα (5 nM) to NLS (30 nM). Data represent the mean ± SEM (n = 4) from a single experiment from a series of 3 independent experiments; (c) Intrinsic tryptophan fluorescence spectra of PfIMPα, TgIMPα and MmIMPα were collected in the presence or absence of Bay 11-7082 and Bay 11-7085 at indicated concentration ranges; (d) Compound concentrations vs changes in fluorescence intensity with Bay 11-7082 and Bay 11-7085 at a single fluorescence wavelength ( λ max) were plotted using GraphPad prism. Data were plotted as mean ± SD from three independent experiments for each assessment; (e) Cell viability assay of T. gondii tachyzoites when treated with Bay 11-7082. RLUs represent the relative light units from a strain stably expressing firefly luciferase; (f) MTT assay of <t>HFF</t> <t>cells.</t>
    Confluent Hff Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Importin α inhibitors act against the differentiated stages of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii"

    Article Title: Importin α inhibitors act against the differentiated stages of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii

    Journal: bioRxiv

    doi: 10.1101/2024.07.10.602875

    (a) Structure of Bay 11-7082 and Bay 11-7085 from PubChem ( https://pubchem.ncbi.nlm.nih.gov ); (b) Bay 11-7082 inhibits interactions between apicomplexan IMPα and NLS as shown by AlphaScreen. AlphaScreen technology was used to determine the IC 50 for inhibition by Bay 11-7082 of IMPα (5 nM) to NLS (30 nM). Data represent the mean ± SEM (n = 4) from a single experiment from a series of 3 independent experiments; (c) Intrinsic tryptophan fluorescence spectra of PfIMPα, TgIMPα and MmIMPα were collected in the presence or absence of Bay 11-7082 and Bay 11-7085 at indicated concentration ranges; (d) Compound concentrations vs changes in fluorescence intensity with Bay 11-7082 and Bay 11-7085 at a single fluorescence wavelength ( λ max) were plotted using GraphPad prism. Data were plotted as mean ± SD from three independent experiments for each assessment; (e) Cell viability assay of T. gondii tachyzoites when treated with Bay 11-7082. RLUs represent the relative light units from a strain stably expressing firefly luciferase; (f) MTT assay of HFF cells.
    Figure Legend Snippet: (a) Structure of Bay 11-7082 and Bay 11-7085 from PubChem ( https://pubchem.ncbi.nlm.nih.gov ); (b) Bay 11-7082 inhibits interactions between apicomplexan IMPα and NLS as shown by AlphaScreen. AlphaScreen technology was used to determine the IC 50 for inhibition by Bay 11-7082 of IMPα (5 nM) to NLS (30 nM). Data represent the mean ± SEM (n = 4) from a single experiment from a series of 3 independent experiments; (c) Intrinsic tryptophan fluorescence spectra of PfIMPα, TgIMPα and MmIMPα were collected in the presence or absence of Bay 11-7082 and Bay 11-7085 at indicated concentration ranges; (d) Compound concentrations vs changes in fluorescence intensity with Bay 11-7082 and Bay 11-7085 at a single fluorescence wavelength ( λ max) were plotted using GraphPad prism. Data were plotted as mean ± SD from three independent experiments for each assessment; (e) Cell viability assay of T. gondii tachyzoites when treated with Bay 11-7082. RLUs represent the relative light units from a strain stably expressing firefly luciferase; (f) MTT assay of HFF cells.

    Techniques Used: Amplified Luminescent Proximity Homogenous Assay, Inhibition, Fluorescence, Concentration Assay, Viability Assay, Stable Transfection, Expressing, Luciferase, MTT Assay


    Figure Legend Snippet:

    Techniques Used: In Vitro, In Vivo, Concentration Assay

    (a) Schematic of drug assay. Compounds were added at the same time as alkaline stress or after allowing bradyzoite conversion for 48 hours. The alkaline pH was maintained till the end of the experiments and compounds were added to the fresh media; (b) Bradyzoites (DMSO control) visualised using confocal microscopy after DBA-lectin:FITC and DAPI staining with 63X and 10X magnification; (c) Effect of drugs on bradyzoite differentiation when added at the tachyzoite stages; (d) Effect of drugs on differentiated bradyzoite cysts. For (c) and (d), data were obtained at 24 hours post-treatment and are plotted as a percentage of bradyzoite cysts counted per field (number of bradyzoites [FITC colocalising with DAPI]/total number of cells [DAPI] x100). * indicates a significant difference of p < 0.05 compared to vehicle control. Note that only fields having > 100 HFF cells were included in the analysis.
    Figure Legend Snippet: (a) Schematic of drug assay. Compounds were added at the same time as alkaline stress or after allowing bradyzoite conversion for 48 hours. The alkaline pH was maintained till the end of the experiments and compounds were added to the fresh media; (b) Bradyzoites (DMSO control) visualised using confocal microscopy after DBA-lectin:FITC and DAPI staining with 63X and 10X magnification; (c) Effect of drugs on bradyzoite differentiation when added at the tachyzoite stages; (d) Effect of drugs on differentiated bradyzoite cysts. For (c) and (d), data were obtained at 24 hours post-treatment and are plotted as a percentage of bradyzoite cysts counted per field (number of bradyzoites [FITC colocalising with DAPI]/total number of cells [DAPI] x100). * indicates a significant difference of p < 0.05 compared to vehicle control. Note that only fields having > 100 HFF cells were included in the analysis.

    Techniques Used: Control, Confocal Microscopy, Staining

    hffs  (Agilent technologies)


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    Agilent technologies hffs
    Hffs, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GraphPad Software Inc hff
    (a) Schematic of drug assay. Compounds were added at the same time as alkaline stress or after <t>allowing</t> <t>bradyzoite</t> conversion for 48 hours. The alkaline pH was maintained till the end of the experiments and compounds were added to the fresh media; (b) Bradyzoites (DMSO control) visualised using confocal microscopy after DBA-lectin:FITC and DAPI staining with 63X and 10X magnification; (c) Effect of drugs on bradyzoite differentiation when added at the tachyzoite stages; (d) Effect of drugs on differentiated bradyzoite cysts. For (c) and (d), data were obtained at 24 hours post-treatment and are plotted as a percentage of bradyzoite cysts counted per field (number of bradyzoites [FITC colocalising with DAPI]/total number of cells [DAPI] x100). * indicates a significant difference of p < 0.05 compared to vehicle control. Note that only fields having > 100 <t>HFF</t> cells were included in the analysis.
    Hff, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Importin α inhibitors act against the differentiated stages of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii"

    Article Title: Importin α inhibitors act against the differentiated stages of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii

    Journal: bioRxiv

    doi: 10.1101/2024.07.10.602875

    (a) Schematic of drug assay. Compounds were added at the same time as alkaline stress or after allowing bradyzoite conversion for 48 hours. The alkaline pH was maintained till the end of the experiments and compounds were added to the fresh media; (b) Bradyzoites (DMSO control) visualised using confocal microscopy after DBA-lectin:FITC and DAPI staining with 63X and 10X magnification; (c) Effect of drugs on bradyzoite differentiation when added at the tachyzoite stages; (d) Effect of drugs on differentiated bradyzoite cysts. For (c) and (d), data were obtained at 24 hours post-treatment and are plotted as a percentage of bradyzoite cysts counted per field (number of bradyzoites [FITC colocalising with DAPI]/total number of cells [DAPI] x100). * indicates a significant difference of p < 0.05 compared to vehicle control. Note that only fields having > 100 HFF cells were included in the analysis.
    Figure Legend Snippet: (a) Schematic of drug assay. Compounds were added at the same time as alkaline stress or after allowing bradyzoite conversion for 48 hours. The alkaline pH was maintained till the end of the experiments and compounds were added to the fresh media; (b) Bradyzoites (DMSO control) visualised using confocal microscopy after DBA-lectin:FITC and DAPI staining with 63X and 10X magnification; (c) Effect of drugs on bradyzoite differentiation when added at the tachyzoite stages; (d) Effect of drugs on differentiated bradyzoite cysts. For (c) and (d), data were obtained at 24 hours post-treatment and are plotted as a percentage of bradyzoite cysts counted per field (number of bradyzoites [FITC colocalising with DAPI]/total number of cells [DAPI] x100). * indicates a significant difference of p < 0.05 compared to vehicle control. Note that only fields having > 100 HFF cells were included in the analysis.

    Techniques Used: Control, Confocal Microscopy, Staining

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    Thermo Fisher irradiated human foreskin fibroblasts irr hff
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    (a) Structure of Bay 11-7082 and Bay 11-7085 from PubChem ( https://pubchem.ncbi.nlm.nih.gov ); (b) Bay 11-7082 inhibits interactions between apicomplexan IMPα and NLS as shown by AlphaScreen. AlphaScreen technology was used to determine the IC 50 for inhibition by Bay 11-7082 of IMPα (5 nM) to NLS (30 nM). Data represent the mean ± SEM (n = 4) from a single experiment from a series of 3 independent experiments; (c) Intrinsic tryptophan fluorescence spectra of PfIMPα, TgIMPα and MmIMPα were collected in the presence or absence of Bay 11-7082 and Bay 11-7085 at indicated concentration ranges; (d) Compound concentrations vs changes in fluorescence intensity with Bay 11-7082 and Bay 11-7085 at a single fluorescence wavelength ( λ max) were plotted using GraphPad prism. Data were plotted as mean ± SD from three independent experiments for each assessment; (e) Cell viability assay of T. gondii tachyzoites when treated with Bay 11-7082. RLUs represent the relative light units from a strain stably expressing firefly luciferase; (f) MTT assay of <t>HFF</t> <t>cells.</t>
    Confluent Hff Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (a) Structure of Bay 11-7082 and Bay 11-7085 from PubChem ( https://pubchem.ncbi.nlm.nih.gov ); (b) Bay 11-7082 inhibits interactions between apicomplexan IMPα and NLS as shown by AlphaScreen. AlphaScreen technology was used to determine the IC 50 for inhibition by Bay 11-7082 of IMPα (5 nM) to NLS (30 nM). Data represent the mean ± SEM (n = 4) from a single experiment from a series of 3 independent experiments; (c) Intrinsic tryptophan fluorescence spectra of PfIMPα, TgIMPα and MmIMPα were collected in the presence or absence of Bay 11-7082 and Bay 11-7085 at indicated concentration ranges; (d) Compound concentrations vs changes in fluorescence intensity with Bay 11-7082 and Bay 11-7085 at a single fluorescence wavelength ( λ max) were plotted using GraphPad prism. Data were plotted as mean ± SD from three independent experiments for each assessment; (e) Cell viability assay of T. gondii tachyzoites when treated with Bay 11-7082. RLUs represent the relative light units from a strain stably expressing firefly luciferase; (f) MTT assay of <t>HFF</t> <t>cells.</t>
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    (a) Schematic of drug assay. Compounds were added at the same time as alkaline stress or after <t>allowing</t> <t>bradyzoite</t> conversion for 48 hours. The alkaline pH was maintained till the end of the experiments and compounds were added to the fresh media; (b) Bradyzoites (DMSO control) visualised using confocal microscopy after DBA-lectin:FITC and DAPI staining with 63X and 10X magnification; (c) Effect of drugs on bradyzoite differentiation when added at the tachyzoite stages; (d) Effect of drugs on differentiated bradyzoite cysts. For (c) and (d), data were obtained at 24 hours post-treatment and are plotted as a percentage of bradyzoite cysts counted per field (number of bradyzoites [FITC colocalising with DAPI]/total number of cells [DAPI] x100). * indicates a significant difference of p < 0.05 compared to vehicle control. Note that only fields having > 100 <t>HFF</t> cells were included in the analysis.
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    Image Search Results


    (a) Structure of Bay 11-7082 and Bay 11-7085 from PubChem ( https://pubchem.ncbi.nlm.nih.gov ); (b) Bay 11-7082 inhibits interactions between apicomplexan IMPα and NLS as shown by AlphaScreen. AlphaScreen technology was used to determine the IC 50 for inhibition by Bay 11-7082 of IMPα (5 nM) to NLS (30 nM). Data represent the mean ± SEM (n = 4) from a single experiment from a series of 3 independent experiments; (c) Intrinsic tryptophan fluorescence spectra of PfIMPα, TgIMPα and MmIMPα were collected in the presence or absence of Bay 11-7082 and Bay 11-7085 at indicated concentration ranges; (d) Compound concentrations vs changes in fluorescence intensity with Bay 11-7082 and Bay 11-7085 at a single fluorescence wavelength ( λ max) were plotted using GraphPad prism. Data were plotted as mean ± SD from three independent experiments for each assessment; (e) Cell viability assay of T. gondii tachyzoites when treated with Bay 11-7082. RLUs represent the relative light units from a strain stably expressing firefly luciferase; (f) MTT assay of HFF cells.

    Journal: bioRxiv

    Article Title: Importin α inhibitors act against the differentiated stages of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii

    doi: 10.1101/2024.07.10.602875

    Figure Lengend Snippet: (a) Structure of Bay 11-7082 and Bay 11-7085 from PubChem ( https://pubchem.ncbi.nlm.nih.gov ); (b) Bay 11-7082 inhibits interactions between apicomplexan IMPα and NLS as shown by AlphaScreen. AlphaScreen technology was used to determine the IC 50 for inhibition by Bay 11-7082 of IMPα (5 nM) to NLS (30 nM). Data represent the mean ± SEM (n = 4) from a single experiment from a series of 3 independent experiments; (c) Intrinsic tryptophan fluorescence spectra of PfIMPα, TgIMPα and MmIMPα were collected in the presence or absence of Bay 11-7082 and Bay 11-7085 at indicated concentration ranges; (d) Compound concentrations vs changes in fluorescence intensity with Bay 11-7082 and Bay 11-7085 at a single fluorescence wavelength ( λ max) were plotted using GraphPad prism. Data were plotted as mean ± SD from three independent experiments for each assessment; (e) Cell viability assay of T. gondii tachyzoites when treated with Bay 11-7082. RLUs represent the relative light units from a strain stably expressing firefly luciferase; (f) MTT assay of HFF cells.

    Article Snippet: Confluent HFF cells were infected with ME49ΔKu80 parasites (1 parasite: 5 hosts) in DMEM media (Invitrogen) with 10% (v/v) FBS (HiMedia), pH 7.4.

    Techniques: Amplified Luminescent Proximity Homogenous Assay, Inhibition, Fluorescence, Concentration Assay, Viability Assay, Stable Transfection, Expressing, Luciferase, MTT Assay

    Journal: bioRxiv

    Article Title: Importin α inhibitors act against the differentiated stages of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii

    doi: 10.1101/2024.07.10.602875

    Figure Lengend Snippet:

    Article Snippet: Confluent HFF cells were infected with ME49ΔKu80 parasites (1 parasite: 5 hosts) in DMEM media (Invitrogen) with 10% (v/v) FBS (HiMedia), pH 7.4.

    Techniques: In Vitro, In Vivo, Concentration Assay

    (a) Schematic of drug assay. Compounds were added at the same time as alkaline stress or after allowing bradyzoite conversion for 48 hours. The alkaline pH was maintained till the end of the experiments and compounds were added to the fresh media; (b) Bradyzoites (DMSO control) visualised using confocal microscopy after DBA-lectin:FITC and DAPI staining with 63X and 10X magnification; (c) Effect of drugs on bradyzoite differentiation when added at the tachyzoite stages; (d) Effect of drugs on differentiated bradyzoite cysts. For (c) and (d), data were obtained at 24 hours post-treatment and are plotted as a percentage of bradyzoite cysts counted per field (number of bradyzoites [FITC colocalising with DAPI]/total number of cells [DAPI] x100). * indicates a significant difference of p < 0.05 compared to vehicle control. Note that only fields having > 100 HFF cells were included in the analysis.

    Journal: bioRxiv

    Article Title: Importin α inhibitors act against the differentiated stages of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii

    doi: 10.1101/2024.07.10.602875

    Figure Lengend Snippet: (a) Schematic of drug assay. Compounds were added at the same time as alkaline stress or after allowing bradyzoite conversion for 48 hours. The alkaline pH was maintained till the end of the experiments and compounds were added to the fresh media; (b) Bradyzoites (DMSO control) visualised using confocal microscopy after DBA-lectin:FITC and DAPI staining with 63X and 10X magnification; (c) Effect of drugs on bradyzoite differentiation when added at the tachyzoite stages; (d) Effect of drugs on differentiated bradyzoite cysts. For (c) and (d), data were obtained at 24 hours post-treatment and are plotted as a percentage of bradyzoite cysts counted per field (number of bradyzoites [FITC colocalising with DAPI]/total number of cells [DAPI] x100). * indicates a significant difference of p < 0.05 compared to vehicle control. Note that only fields having > 100 HFF cells were included in the analysis.

    Article Snippet: Confluent HFF cells were infected with ME49ΔKu80 parasites (1 parasite: 5 hosts) in DMEM media (Invitrogen) with 10% (v/v) FBS (HiMedia), pH 7.4.

    Techniques: Control, Confocal Microscopy, Staining

    (a) Schematic of drug assay. Compounds were added at the same time as alkaline stress or after allowing bradyzoite conversion for 48 hours. The alkaline pH was maintained till the end of the experiments and compounds were added to the fresh media; (b) Bradyzoites (DMSO control) visualised using confocal microscopy after DBA-lectin:FITC and DAPI staining with 63X and 10X magnification; (c) Effect of drugs on bradyzoite differentiation when added at the tachyzoite stages; (d) Effect of drugs on differentiated bradyzoite cysts. For (c) and (d), data were obtained at 24 hours post-treatment and are plotted as a percentage of bradyzoite cysts counted per field (number of bradyzoites [FITC colocalising with DAPI]/total number of cells [DAPI] x100). * indicates a significant difference of p < 0.05 compared to vehicle control. Note that only fields having > 100 HFF cells were included in the analysis.

    Journal: bioRxiv

    Article Title: Importin α inhibitors act against the differentiated stages of apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii

    doi: 10.1101/2024.07.10.602875

    Figure Lengend Snippet: (a) Schematic of drug assay. Compounds were added at the same time as alkaline stress or after allowing bradyzoite conversion for 48 hours. The alkaline pH was maintained till the end of the experiments and compounds were added to the fresh media; (b) Bradyzoites (DMSO control) visualised using confocal microscopy after DBA-lectin:FITC and DAPI staining with 63X and 10X magnification; (c) Effect of drugs on bradyzoite differentiation when added at the tachyzoite stages; (d) Effect of drugs on differentiated bradyzoite cysts. For (c) and (d), data were obtained at 24 hours post-treatment and are plotted as a percentage of bradyzoite cysts counted per field (number of bradyzoites [FITC colocalising with DAPI]/total number of cells [DAPI] x100). * indicates a significant difference of p < 0.05 compared to vehicle control. Note that only fields having > 100 HFF cells were included in the analysis.

    Article Snippet: Graphs were plotted in GraphPad Prism (Version 8.4.3) (San Diego, California, USA) after calculating the percentage of bradyzoite cysts per HFF in a field.

    Techniques: Control, Confocal Microscopy, Staining