Journal: International Journal of Nanomedicine

Article Title: Tarin-Loaded Nanoliposomes Activate Apoptosis and Autophagy and Inhibit the Migration of Human Mammary Adenocarcinoma Cells

doi: 10.2147/IJN.S434626

Figure Lengend Snippet: Viability of healthy human cells (HFF-1) exposed to nanoliposome-encapsulated tarin (upper panels) and free tarin (bottom panels) for 24 h and 48 h. The viability of HFF-1 cells in cultures was determined employing resazurin as an indicator. The x-axis in the upper panel refers to the encapsulated tarin concentration, while empty liposomes refer to the corresponding volume of liposomal solution used to reach that concentration. *p< 0.05 compared to the control. ns – non-significant relative to the control.

Article Snippet: Healthy HFF-1 line human fibroblasts (ATCC SCRC-1041) and MDA-MB-231 line human mammary adenocarcinoma cells (ATCC HTB-26) were obtained from the Rio de Janeiro Cell Bank (BCRJ), RJ, BRA available at https://bcrj.org.br/ .

Techniques: Concentration Assay, Liposomes

Journal: Scientific Reports

Article Title: The study of tryptophol containing emulgel on fungal reduction and skin irritation

doi: 10.1038/s41598-023-46121-z

Figure Lengend Snippet: Cell viability ( a ) and cytotoxicity ( b ) of TOH-treated human foreskin fibroblasts (HFF- 1) cells at 37 °C as determined by MTT, Mitogreen, LDH, and dual AO/EB staining assays. Percentages of cell viability and cytotoxicity were shown as mean ± SD. *P < 0.05 and ****P < 0.0001 indicate significant differences compared with the control group (concentration 0). TOH Tryptophol, VOZ voriconazole.

Article Snippet: Human foreskin fibroblasts (HFF-1) cell line (ATCC #SCRC-1041™) was used to investigate cell viability and cytotoxicity after TOH treatments.

Techniques: Staining, Concentration Assay

Journal: Journal of Virology

Article Title: Human cytomegalovirus mediates APOBEC3B relocalization early during infection through a ribonucleotide reductase-independent mechanism

doi: 10.1128/jvi.00781-23

Figure Lengend Snippet: A3B relocalization occurs early during HCMV infection. ( A and C ) Representative IF microscopy images of HFF-1 cells stably expressing A3B-HA incubated with medium alone (mock) or infected with the indicated HCMV strains for the indicated timepoints (10 µm scale). ( B and D ) Quantification of A3B-HA subcellular localization phenotypes shown in panels A and C. Each histogram bar reports the percentage of cells with whole cell, cytoplasmic, and nuclear A3B-HA ( n > 100 cells per condition; mean ± SD with P values from unpaired Student’s t -tests).

Article Snippet: HFF-1 (ATCC, Manassas, VA, USA), U373 (ATCC), and 293T cells were cultured in Dulbecco's Modified Eagle Medium (DMEM; Cytiva, Marlborough, MA, USA) supplemented with 10% fetal bovine serum (Gibco, Billings, MT, USA) and 1% penicillin/streptomycin (Gibco).

Techniques: Infection, Microscopy, Stable Transfection, Expressing, Incubation

Journal: Journal of Virology

Article Title: Human cytomegalovirus mediates APOBEC3B relocalization early during infection through a ribonucleotide reductase-independent mechanism

doi: 10.1128/jvi.00781-23

Figure Lengend Snippet: A3B relocalization requires de novo translation of HCMV proteins but not viral DNA synthesis. ( A ) Schematic representation of experimental workflows of CHX and PAA treatment of infected cells. Image created with BioRender. ( B ) Representative IF microscopy images of HFF-1 cells stably expressing A3B-HA incubated with medium alone (mock) or infected with AD169-GFP and treated with DMSO or CHX for 24 h (10 µm scale). ( C ) Representative IF microscopy images of U373 cells stably expressing A3B-HA incubated with medium alone (mock) or infected with AD169-GFP or AD169-GFP ΔIE1 for 72 h (10 µm scale). ( D ) Representative IF microscopy images of HFF-1 cells stably expressing A3B-HA incubated with medium alone (mock) or infected with AD169-GFP and treated with DMSO or PAA for 48 h (10 µm scale). ( E ) Quantification of A3B-HA subcellular localization phenotypes shown in panels B and D. Each histogram bar reports the percentage of cells with cytoplasmic A3B-HA ( n > 80 cells per condition; mean ± SD with P values from unpaired Student’s t -tests).

Article Snippet: HFF-1 (ATCC, Manassas, VA, USA), U373 (ATCC), and 293T cells were cultured in Dulbecco's Modified Eagle Medium (DMEM; Cytiva, Marlborough, MA, USA) supplemented with 10% fetal bovine serum (Gibco, Billings, MT, USA) and 1% penicillin/streptomycin (Gibco).

Techniques: DNA Synthesis, Infection, Microscopy, Stable Transfection, Expressing, Incubation