hff 1 cells  (ATCC)


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    ATCC hff 1 cells
    Hff 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human foreskin fibroblast 1 hff 1 cell line  (ATCC)


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    ATCC human foreskin fibroblast 1 hff 1 cell line
    Human Foreskin Fibroblast 1 Hff 1 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hff 1 cells  (ATCC)


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    ATCC hff 1 cells
    Hff 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human foreskin fibroblast cell line hff 1  (ATCC)


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    ATCC human foreskin fibroblast cell line hff 1
    Human Foreskin Fibroblast Cell Line Hff 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hff 1 cells  (ATCC)


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    ATCC hff 1 cells
    <t>HFF-1</t> cells infected with HCMV were UV-inactivated. (A) The virus titres of samples taken during inactivation validation were determined with a limit of detection (LoD) of 5 PFU/mL. The before inactivation and UV inactivated samples were performed with biological replicates, n = 3, and the standard deviation is shown. The untreated control sample was performed with n = 1. (B) Immunofluorescence imaging of cells treated with the UV-inactivated infected cells was performed with biological replicates, n = 3. Cells were stained for the HCMV proteins IE1/2 and with DAPI. The scale bar represents 100 μm.
    Hff 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "A validated protocol to UV-inactivate SARS-CoV-2 and herpesvirus-infected cells"

    Article Title: A validated protocol to UV-inactivate SARS-CoV-2 and herpesvirus-infected cells

    Journal: PLOS ONE

    doi: 10.1371/journal.pone.0274065

    HFF-1 cells infected with HCMV were UV-inactivated. (A) The virus titres of samples taken during inactivation validation were determined with a limit of detection (LoD) of 5 PFU/mL. The before inactivation and UV inactivated samples were performed with biological replicates, n = 3, and the standard deviation is shown. The untreated control sample was performed with n = 1. (B) Immunofluorescence imaging of cells treated with the UV-inactivated infected cells was performed with biological replicates, n = 3. Cells were stained for the HCMV proteins IE1/2 and with DAPI. The scale bar represents 100 μm.
    Figure Legend Snippet: HFF-1 cells infected with HCMV were UV-inactivated. (A) The virus titres of samples taken during inactivation validation were determined with a limit of detection (LoD) of 5 PFU/mL. The before inactivation and UV inactivated samples were performed with biological replicates, n = 3, and the standard deviation is shown. The untreated control sample was performed with n = 1. (B) Immunofluorescence imaging of cells treated with the UV-inactivated infected cells was performed with biological replicates, n = 3. Cells were stained for the HCMV proteins IE1/2 and with DAPI. The scale bar represents 100 μm.

    Techniques Used: Infection, Standard Deviation, Immunofluorescence, Imaging, Staining

    hff 1 cells  (ATCC)


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    ATCC hff 1 cells
    Broad-spectrum antiviral screen (A) Schematic of antiviral screen performed using molecules modulating PAMPs. (B) Immunofluorescence images of human fibroblasts <t>(HFF-1)</t> infected with indicated viruses are shown. CHIKV (MOI: 0.1) and ZIKV (MOI: 0.1) or WNV (MOI: 1) were detected by anti-E1 protein and anti-flavivirus E protein antibodies, respectively. Scale bar: 25 μm. (C) Graph shows the percentage of infectivity of various compounds targeting CHIKV and flaviviruses. Horizontal dotted line indicates 50% infectivity. (D) Immunofluorescence images show phospho-IRF3 (S386) and phospho-STING (S366) in fibroblast after 2-h post-stimulation with indicated compounds. Note: scleroglucan-stimulated cells do not have detectable level of phospho-STING, and only a few cells have a cytoplasmic form of phospho-IRF3. Scale bar: 10 μm. See also <xref ref-type=Figures S1 and and Table S1 . " width="250" height="auto" />
    Hff 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Innate immune pathway modulator screen identifies STING pathway activation as a strategy to inhibit multiple families of arbo and respiratory viruses"

    Article Title: Innate immune pathway modulator screen identifies STING pathway activation as a strategy to inhibit multiple families of arbo and respiratory viruses

    Journal: Cell Reports Medicine

    doi: 10.1016/j.xcrm.2023.101024

    Broad-spectrum antiviral screen (A) Schematic of antiviral screen performed using molecules modulating PAMPs. (B) Immunofluorescence images of human fibroblasts (HFF-1) infected with indicated viruses are shown. CHIKV (MOI: 0.1) and ZIKV (MOI: 0.1) or WNV (MOI: 1) were detected by anti-E1 protein and anti-flavivirus E protein antibodies, respectively. Scale bar: 25 μm. (C) Graph shows the percentage of infectivity of various compounds targeting CHIKV and flaviviruses. Horizontal dotted line indicates 50% infectivity. (D) Immunofluorescence images show phospho-IRF3 (S386) and phospho-STING (S366) in fibroblast after 2-h post-stimulation with indicated compounds. Note: scleroglucan-stimulated cells do not have detectable level of phospho-STING, and only a few cells have a cytoplasmic form of phospho-IRF3. Scale bar: 10 μm. See also <xref ref-type=Figures S1 and and Table S1 . " title="... modulating PAMPs. (B) Immunofluorescence images of human fibroblasts (HFF-1) infected with indicated viruses are shown. CHIKV (MOI: ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Broad-spectrum antiviral screen (A) Schematic of antiviral screen performed using molecules modulating PAMPs. (B) Immunofluorescence images of human fibroblasts (HFF-1) infected with indicated viruses are shown. CHIKV (MOI: 0.1) and ZIKV (MOI: 0.1) or WNV (MOI: 1) were detected by anti-E1 protein and anti-flavivirus E protein antibodies, respectively. Scale bar: 25 μm. (C) Graph shows the percentage of infectivity of various compounds targeting CHIKV and flaviviruses. Horizontal dotted line indicates 50% infectivity. (D) Immunofluorescence images show phospho-IRF3 (S386) and phospho-STING (S366) in fibroblast after 2-h post-stimulation with indicated compounds. Note: scleroglucan-stimulated cells do not have detectable level of phospho-STING, and only a few cells have a cytoplasmic form of phospho-IRF3. Scale bar: 10 μm. See also Figures S1 and and Table S1 .

    Techniques Used: Immunofluorescence, Infection

    Comparative transcriptome analysis of drug-treated and arbovirus-infected human fibroblasts (A) Violin plot shows comparative analysis of differentially expressed genes of vehicle- or cAIMP-treated virus-infected HFF-1 cells at 24 hpi. (B) Venn diagrams display the number of common and distinctive genes upregulated in virus-infected cells (left) as well as in cAIMP-treated virus-infected cells (right) at 24 hpi (false discovery rate [FDR] < 0.01 and log 2 fold change [log2FC] > 1). (C) Graphs show qRT-PCR analysis of innate immune STING pathway genes in arbovirus-infected fibroblasts at 48 hpi with or without drug treatments of infected cells. Student’s t test. ∗p > 0.01, ∗∗p > 0.001, ∗∗∗p > 0.0001. (D and E) Dot plot analysis of overrepresented pathways in viral-infected (D) and cAIMP-treated virus-infected cells (E). The size of the dot represents the fold enrichment (FoldEnrich), while its color represents the FDR (p adjusted) value for each enriched Reactome pathway. The x axis represents the percentage of upregulated genes in the selected pathway that is presented in the y axis. See also <xref ref-type=Figure S3 and Tables S2 and . " title="... differentially expressed genes of vehicle- or cAIMP-treated virus-infected HFF-1 cells at 24 hpi. (B) Venn diagrams display ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Comparative transcriptome analysis of drug-treated and arbovirus-infected human fibroblasts (A) Violin plot shows comparative analysis of differentially expressed genes of vehicle- or cAIMP-treated virus-infected HFF-1 cells at 24 hpi. (B) Venn diagrams display the number of common and distinctive genes upregulated in virus-infected cells (left) as well as in cAIMP-treated virus-infected cells (right) at 24 hpi (false discovery rate [FDR] < 0.01 and log 2 fold change [log2FC] > 1). (C) Graphs show qRT-PCR analysis of innate immune STING pathway genes in arbovirus-infected fibroblasts at 48 hpi with or without drug treatments of infected cells. Student’s t test. ∗p > 0.01, ∗∗p > 0.001, ∗∗∗p > 0.0001. (D and E) Dot plot analysis of overrepresented pathways in viral-infected (D) and cAIMP-treated virus-infected cells (E). The size of the dot represents the fold enrichment (FoldEnrich), while its color represents the FDR (p adjusted) value for each enriched Reactome pathway. The x axis represents the percentage of upregulated genes in the selected pathway that is presented in the y axis. See also Figure S3 and Tables S2 and .

    Techniques Used: Infection, Quantitative RT-PCR


    Figure Legend Snippet:

    Techniques Used: Produced, Recombinant, Electron Microscopy, Cell Culture, Cell Viability Assay, Infection, Expressing, Derivative Assay, In Vivo, Software

    human fibroblast hff 1 cells  (ATCC)


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    ATCC human fibroblast hff 1 cells
    Cell viability of different doses of imeglimin upon 24 h <t>HFF-1</t> cells exposure. The data demonstrated that imeglimin is safe at ≤250 µg/mL. Results are presented as mean ± SD (n = 3).
    Human Fibroblast Hff 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Development of Imeglimin Electrospun Nanofibers as a Potential Buccal Antidiabetic Therapeutic Approach"

    Article Title: Development of Imeglimin Electrospun Nanofibers as a Potential Buccal Antidiabetic Therapeutic Approach

    Journal: Pharmaceutics

    doi: 10.3390/pharmaceutics15041208

    Cell viability of different doses of imeglimin upon 24 h HFF-1 cells exposure. The data demonstrated that imeglimin is safe at ≤250 µg/mL. Results are presented as mean ± SD (n = 3).
    Figure Legend Snippet: Cell viability of different doses of imeglimin upon 24 h HFF-1 cells exposure. The data demonstrated that imeglimin is safe at ≤250 µg/mL. Results are presented as mean ± SD (n = 3).

    Techniques Used:

    human fibroblast cell lineage hff 1  (ATCC)


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    ATCC human fibroblast cell lineage hff 1
    Human Fibroblast Cell Lineage Hff 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hff 1 cells  (ATCC)


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    ATCC hff 1 cells
    Hff 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hff 1 cells  (ATCC)


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    ATCC hff 1 cells
    Biocompatibility of PHO active films. ( A ) viability test on <t>HFF-1</t> cells after 24 h of exposure; ( B ) genotoxicity assay on HFF-1 cells after 3 h of exposure; ( C ) hemolysis caused by neat PHO films and bioactive PHO films; and ( D ) platelet aggregation of human plasma on active PHO and neat PHO films after 3 min of exposure. Mean values with different letters represent significant differences ( p < 0.05). C− (negative control), C+ (positive control), SS (saline solution), and DW (distilled water).
    Hff 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Microbially Synthesized Polymer-Metal Nanoparticles Composites as Promising Wound Dressings to Overcome Methicillin-Resistance Staphylococcus aureus Infections"

    Article Title: Microbially Synthesized Polymer-Metal Nanoparticles Composites as Promising Wound Dressings to Overcome Methicillin-Resistance Staphylococcus aureus Infections

    Journal: Polymers

    doi: 10.3390/polym15040920

    Biocompatibility of PHO active films. ( A ) viability test on HFF-1 cells after 24 h of exposure; ( B ) genotoxicity assay on HFF-1 cells after 3 h of exposure; ( C ) hemolysis caused by neat PHO films and bioactive PHO films; and ( D ) platelet aggregation of human plasma on active PHO and neat PHO films after 3 min of exposure. Mean values with different letters represent significant differences ( p < 0.05). C− (negative control), C+ (positive control), SS (saline solution), and DW (distilled water).
    Figure Legend Snippet: Biocompatibility of PHO active films. ( A ) viability test on HFF-1 cells after 24 h of exposure; ( B ) genotoxicity assay on HFF-1 cells after 3 h of exposure; ( C ) hemolysis caused by neat PHO films and bioactive PHO films; and ( D ) platelet aggregation of human plasma on active PHO and neat PHO films after 3 min of exposure. Mean values with different letters represent significant differences ( p < 0.05). C− (negative control), C+ (positive control), SS (saline solution), and DW (distilled water).

    Techniques Used: Negative Control, Positive Control

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    ATCC hff 1 cells
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    human foreskin fibroblast 1 hff 1 cell line  (ATCC)
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    ATCC human foreskin fibroblast 1 hff 1 cell line
    Human Foreskin Fibroblast 1 Hff 1 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human foreskin fibroblast cell line hff 1  (ATCC)
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    ATCC human foreskin fibroblast cell line hff 1
    Human Foreskin Fibroblast Cell Line Hff 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human fibroblast hff 1 cells  (ATCC)
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    ATCC human fibroblast hff 1 cells
    Cell viability of different doses of imeglimin upon 24 h <t>HFF-1</t> cells exposure. The data demonstrated that imeglimin is safe at ≤250 µg/mL. Results are presented as mean ± SD (n = 3).
    Human Fibroblast Hff 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human fibroblast cell lineage hff 1  (ATCC)
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    ATCC human fibroblast cell lineage hff 1
    Cell viability of different doses of imeglimin upon 24 h <t>HFF-1</t> cells exposure. The data demonstrated that imeglimin is safe at ≤250 µg/mL. Results are presented as mean ± SD (n = 3).
    Human Fibroblast Cell Lineage Hff 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell viability of different doses of imeglimin upon 24 h HFF-1 cells exposure. The data demonstrated that imeglimin is safe at ≤250 µg/mL. Results are presented as mean ± SD (n = 3).

    Journal: Pharmaceutics

    Article Title: Development of Imeglimin Electrospun Nanofibers as a Potential Buccal Antidiabetic Therapeutic Approach

    doi: 10.3390/pharmaceutics15041208

    Figure Lengend Snippet: Cell viability of different doses of imeglimin upon 24 h HFF-1 cells exposure. The data demonstrated that imeglimin is safe at ≤250 µg/mL. Results are presented as mean ± SD (n = 3).

    Article Snippet: It measured the cellular metabolic activity of human fibroblast HFF-1 cells (ATCC- SCRC-1401) following a 24 h exposure to the tested drug.

    Techniques: