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Cell proliferation analysis to silence of FOXA1 in ovarian cancer cells. FOXA1 silencing in SK-OV3 and HEYA8 ovarian cancer cell lines reduces cell proliferation compared to control. (* p < 0.01).

Journal: International Journal of Molecular Sciences

Article Title: FOXA1 in Ovarian Cancer: A Potential Therapeutic Target to Enhance Immunotherapy Efficacy

doi: 10.3390/ijms27031194

Figure Lengend Snippet: Cell proliferation analysis to silence of FOXA1 in ovarian cancer cells. FOXA1 silencing in SK-OV3 and HEYA8 ovarian cancer cell lines reduces cell proliferation compared to control. (* p < 0.01).

Article Snippet: Human ovarian cancer cell line SK-OV3 (high-grade serous ovarian cancer cell line) and HEYA8 (metastatic ovarian cancer cell line) were obtained from the Korean Cell Line Bank (KCLB, Seoul, Republic of Korea).

Techniques: Control

Cell migration, invasion, and wound healing assay to silence of FOXA1 in ovarian cancer cells. ( a ) FOXA1 si decreased the migration ability of ovarian cancer cells (Left: SK-OV3, Right: HEYA8). ( b ) FOXA1 si decreased the invasion ability of ovarian cancer cells (Left: SK-OV3, Right: HEYA8). ( c ) Representative images of the wound healing assay at 0, 12, 24, 36, and 48 h time points (Left: SK-OV3, Right: HEYA8). ( d ) The quantitative evaluation and statistical analysis of wound area percentage in wound healing assay measured by Image J software. Results are expressed as mean ± SD of five experiments (Left: SK-OV3, Right: HEYA8).

Journal: International Journal of Molecular Sciences

Article Title: FOXA1 in Ovarian Cancer: A Potential Therapeutic Target to Enhance Immunotherapy Efficacy

doi: 10.3390/ijms27031194

Figure Lengend Snippet: Cell migration, invasion, and wound healing assay to silence of FOXA1 in ovarian cancer cells. ( a ) FOXA1 si decreased the migration ability of ovarian cancer cells (Left: SK-OV3, Right: HEYA8). ( b ) FOXA1 si decreased the invasion ability of ovarian cancer cells (Left: SK-OV3, Right: HEYA8). ( c ) Representative images of the wound healing assay at 0, 12, 24, 36, and 48 h time points (Left: SK-OV3, Right: HEYA8). ( d ) The quantitative evaluation and statistical analysis of wound area percentage in wound healing assay measured by Image J software. Results are expressed as mean ± SD of five experiments (Left: SK-OV3, Right: HEYA8).

Article Snippet: Human ovarian cancer cell line SK-OV3 (high-grade serous ovarian cancer cell line) and HEYA8 (metastatic ovarian cancer cell line) were obtained from the Korean Cell Line Bank (KCLB, Seoul, Republic of Korea).

Techniques: Migration, Wound Healing Assay, Software

Comparison of EMT gene expression after treatment with FOXA1 siRNA. ( a ) Changes in EMT gene expression in HEYA8. ( b ) Changes in EMT gene expression in SK-OV3. ( c ) Schematic representation of EMT and MET processes and their key molecular markers.

Journal: International Journal of Molecular Sciences

Article Title: FOXA1 in Ovarian Cancer: A Potential Therapeutic Target to Enhance Immunotherapy Efficacy

doi: 10.3390/ijms27031194

Figure Lengend Snippet: Comparison of EMT gene expression after treatment with FOXA1 siRNA. ( a ) Changes in EMT gene expression in HEYA8. ( b ) Changes in EMT gene expression in SK-OV3. ( c ) Schematic representation of EMT and MET processes and their key molecular markers.

Article Snippet: Human ovarian cancer cell line SK-OV3 (high-grade serous ovarian cancer cell line) and HEYA8 (metastatic ovarian cancer cell line) were obtained from the Korean Cell Line Bank (KCLB, Seoul, Republic of Korea).

Techniques: Comparison, Gene Expression

Reduction in chemoresistance following FOXA1 Gene Knockdown. ( a ) Determining IC50 values using carboplatin. ( b ) Atezolizumab cytotoxicity test in SK-OV3 and HEY A8 cells. ( c , d ) Cell viability of SK-OV3 and HEYA8 ovarian cancer cells following carboplatin treatment with or without atezolizumab. ( e , f ) Cell viability of SK-OV3 and HEYA8 ovarian cancer cells following carboplatin treatment with or without FOXA1 knockdown. ( g , h ) Cell viability of SK-OV3 and HEYA8 ovarian cancer cells following combined treatment with carboplatin and atezolizumab, with or without FOXA1 knockdown. ( i , j ) Comparison of cell viability in FOXA1-silenced SK-OV3 and HEYA8 cells treated with carboplatin alone or in combination with atezolizumab.

Journal: International Journal of Molecular Sciences

Article Title: FOXA1 in Ovarian Cancer: A Potential Therapeutic Target to Enhance Immunotherapy Efficacy

doi: 10.3390/ijms27031194

Figure Lengend Snippet: Reduction in chemoresistance following FOXA1 Gene Knockdown. ( a ) Determining IC50 values using carboplatin. ( b ) Atezolizumab cytotoxicity test in SK-OV3 and HEY A8 cells. ( c , d ) Cell viability of SK-OV3 and HEYA8 ovarian cancer cells following carboplatin treatment with or without atezolizumab. ( e , f ) Cell viability of SK-OV3 and HEYA8 ovarian cancer cells following carboplatin treatment with or without FOXA1 knockdown. ( g , h ) Cell viability of SK-OV3 and HEYA8 ovarian cancer cells following combined treatment with carboplatin and atezolizumab, with or without FOXA1 knockdown. ( i , j ) Comparison of cell viability in FOXA1-silenced SK-OV3 and HEYA8 cells treated with carboplatin alone or in combination with atezolizumab.

Article Snippet: Human ovarian cancer cell line SK-OV3 (high-grade serous ovarian cancer cell line) and HEYA8 (metastatic ovarian cancer cell line) were obtained from the Korean Cell Line Bank (KCLB, Seoul, Republic of Korea).

Techniques: Knockdown, Comparison