Structured Review

Millipore hexenol
Concentration of medium sugars (A,B) , and lactate (C,D) . Principle component analysis (PCA) based on 30 VOC GC-MS data of 2D and 3D cultures (E,F) , and absolute quantification of trans <t>-2-hexenol</t> and isobutyrate in 2D-cultured cell-less media and 1 × 10 6 and 2 × 10 6 A549 cells (human lung adenocarcinoma) A549 cells (G,H) . qPCR data (I,J) , ALDH (aldehyde dehydrogenase) activity assay over time (K–M) , and ROS data (N–P) . HLB (Human lung fibroblasts cells), HBEpC (Human bronchial epithelial cells as primary cells), and A549 (human lung adenocarcinoma) were compared. Note different scales on y-axis. (A) Neutral sugar concentration in 2D-cultured medium. (B) Neutral sugar concentration in 3D-cultured medium; Y axis indicates μg Sugars/μL culture medium, error bars indicate standard deviation. (C) Lactate concentration in 2D-cultured medium. (D) Lactate concentration in 3D-cultured medium. Y axis indicates μg Lactate/μL culture medium, error bars indicate standard deviation. Medium without cells, HLB (Human lung fibroblasts cells), HBEpC (Human bronchial epithelial cells as primary cells), and A549 (Human lung adenocarcinoma) were compared. For the sugar and lactate assays, sampling was done at 0, 1, 2, 3, 4 days after inoculation. Experiments were biologically replicated four times. Asterisk indicates statistical significance ( p
Hexenol, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hexenol/product/Millipore
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
hexenol - by Bioz Stars, 2021-01
88/100 stars

Images

1) Product Images from "Elucidation of Biochemical Pathways Underlying VOCs Production in A549 Cells"

Article Title: Elucidation of Biochemical Pathways Underlying VOCs Production in A549 Cells

Journal: Frontiers in Molecular Biosciences

doi: 10.3389/fmolb.2020.00116

Concentration of medium sugars (A,B) , and lactate (C,D) . Principle component analysis (PCA) based on 30 VOC GC-MS data of 2D and 3D cultures (E,F) , and absolute quantification of trans -2-hexenol and isobutyrate in 2D-cultured cell-less media and 1 × 10 6 and 2 × 10 6 A549 cells (human lung adenocarcinoma) A549 cells (G,H) . qPCR data (I,J) , ALDH (aldehyde dehydrogenase) activity assay over time (K–M) , and ROS data (N–P) . HLB (Human lung fibroblasts cells), HBEpC (Human bronchial epithelial cells as primary cells), and A549 (human lung adenocarcinoma) were compared. Note different scales on y-axis. (A) Neutral sugar concentration in 2D-cultured medium. (B) Neutral sugar concentration in 3D-cultured medium; Y axis indicates μg Sugars/μL culture medium, error bars indicate standard deviation. (C) Lactate concentration in 2D-cultured medium. (D) Lactate concentration in 3D-cultured medium. Y axis indicates μg Lactate/μL culture medium, error bars indicate standard deviation. Medium without cells, HLB (Human lung fibroblasts cells), HBEpC (Human bronchial epithelial cells as primary cells), and A549 (Human lung adenocarcinoma) were compared. For the sugar and lactate assays, sampling was done at 0, 1, 2, 3, 4 days after inoculation. Experiments were biologically replicated four times. Asterisk indicates statistical significance ( p
Figure Legend Snippet: Concentration of medium sugars (A,B) , and lactate (C,D) . Principle component analysis (PCA) based on 30 VOC GC-MS data of 2D and 3D cultures (E,F) , and absolute quantification of trans -2-hexenol and isobutyrate in 2D-cultured cell-less media and 1 × 10 6 and 2 × 10 6 A549 cells (human lung adenocarcinoma) A549 cells (G,H) . qPCR data (I,J) , ALDH (aldehyde dehydrogenase) activity assay over time (K–M) , and ROS data (N–P) . HLB (Human lung fibroblasts cells), HBEpC (Human bronchial epithelial cells as primary cells), and A549 (human lung adenocarcinoma) were compared. Note different scales on y-axis. (A) Neutral sugar concentration in 2D-cultured medium. (B) Neutral sugar concentration in 3D-cultured medium; Y axis indicates μg Sugars/μL culture medium, error bars indicate standard deviation. (C) Lactate concentration in 2D-cultured medium. (D) Lactate concentration in 3D-cultured medium. Y axis indicates μg Lactate/μL culture medium, error bars indicate standard deviation. Medium without cells, HLB (Human lung fibroblasts cells), HBEpC (Human bronchial epithelial cells as primary cells), and A549 (Human lung adenocarcinoma) were compared. For the sugar and lactate assays, sampling was done at 0, 1, 2, 3, 4 days after inoculation. Experiments were biologically replicated four times. Asterisk indicates statistical significance ( p

Techniques Used: Concentration Assay, Gas Chromatography-Mass Spectrometry, Cell Culture, Real-time Polymerase Chain Reaction, Activity Assay, Standard Deviation, Sampling

2) Product Images from "Feeding-induced rearrangement of green leaf volatiles reduces moth oviposition"

Article Title: Feeding-induced rearrangement of green leaf volatiles reduces moth oviposition

Journal: eLife

doi: 10.7554/eLife.00421

Calcium activity patterns of the ( Z )-3- and ( E )-2-isomers in the M. sexta antennal lobe (AL). ( A ) View onto the AL (marked by outline) of a Manduca sexta female after bath application with the calcium-sensitive dye calcium-green-AM. Stimulations with the six tested GLVs resulted in the activation of four regions in the AL most probably corresponding to single glomeruli (four ROIs, regions of interest). ( B ) Representative false color-coded images show calcium responses in the AL after odor stimulation. Images are individually scaled to the strongest activation (given by the max value in each image). Time traces show activity of ROI 1, 2, 3 and 4 (n = 10) in response to odor stimulation (2 s; grey bar). Error bars represent standard errors of means. For hexenal and hexenol, stimulations with the ( E )-2-isomers activated ROI 2 significantly stronger than did stimulations with the ( Z )-3-isomers (Wilcoxon signed-rank test: hexenal: p
Figure Legend Snippet: Calcium activity patterns of the ( Z )-3- and ( E )-2-isomers in the M. sexta antennal lobe (AL). ( A ) View onto the AL (marked by outline) of a Manduca sexta female after bath application with the calcium-sensitive dye calcium-green-AM. Stimulations with the six tested GLVs resulted in the activation of four regions in the AL most probably corresponding to single glomeruli (four ROIs, regions of interest). ( B ) Representative false color-coded images show calcium responses in the AL after odor stimulation. Images are individually scaled to the strongest activation (given by the max value in each image). Time traces show activity of ROI 1, 2, 3 and 4 (n = 10) in response to odor stimulation (2 s; grey bar). Error bars represent standard errors of means. For hexenal and hexenol, stimulations with the ( E )-2-isomers activated ROI 2 significantly stronger than did stimulations with the ( Z )-3-isomers (Wilcoxon signed-rank test: hexenal: p

Techniques Used: Activity Assay, Activation Assay

Related Articles

Synthesized:

Article Title: Identification and Characterization of (3Z):(2E)-Hexenal Isomerases from Cucumber
Article Snippet: .. Mass spectra were generated by an Agilent 7200 accurate-mass quadrupole time-of-flight mass spectrometer, operating in electron ionization mode (70 eV) at 230°C and collected with an acquisition rate of 20 scans per second. volatiles were identified and quantified using standard solutions of Z -3-hexenal, Z -3-hexenol, E -2-hexenal, E -2-hexenol and (E,Z )-2,6-nonadienal (Sigma-Aldrich). (Z,Z )-3,6-nonadienal was synthesized from (Z,Z )-3,6-nonadienol (Ventos) as described below. .. Purification of (3Z ):(2E )-Hexenal Isomerase Activity from Cucumber Fruits All procedures (except for the FPLC runs at RT) were performed at 4°C.

Optical Imaging:

Article Title: Feeding-induced rearrangement of green leaf volatiles reduces moth oviposition
Article Snippet: .. Odorants tested with optical imaging Odors were chosen based on the results of a previous study ( ) and on volatile collections of D. wrightii plants performed for this study ( ; (Z )-3- and (E )-2- hexenal, hexenol and hexenyl acetate [Sigma Aldrich, Seelze, Germany]). ..

Generated:

Article Title: Identification and Characterization of (3Z):(2E)-Hexenal Isomerases from Cucumber
Article Snippet: .. Mass spectra were generated by an Agilent 7200 accurate-mass quadrupole time-of-flight mass spectrometer, operating in electron ionization mode (70 eV) at 230°C and collected with an acquisition rate of 20 scans per second. volatiles were identified and quantified using standard solutions of Z -3-hexenal, Z -3-hexenol, E -2-hexenal, E -2-hexenol and (E,Z )-2,6-nonadienal (Sigma-Aldrich). (Z,Z )-3,6-nonadienal was synthesized from (Z,Z )-3,6-nonadienol (Ventos) as described below. .. Purification of (3Z ):(2E )-Hexenal Isomerase Activity from Cucumber Fruits All procedures (except for the FPLC runs at RT) were performed at 4°C.

other:

Article Title: Methyl Jasmonate-Induced Changes of Flavor Profiles During the Processing of Green, Oolong, and Black Tea
Article Snippet: Chemicals The following chemicals and reagents were used for the analyses: benzyl alcohol, benzaldehyde, β-cyclocitral, dihydroactinidiolide, eugenol, geraniol, trans -2-hexenol, trans -2-hexenal, cis -3-hexenol, cis -3-hexenal, β-ionone, jasmine lactone, linalool, methyl salicylate (MeSA), MeJA, 2-phenylethanol, phenylacetaldehyde, and phenylacetonitrile (Sigma–Aldrich, Germany).

Article Title: Analysis of aroma-active compounds in three sweet osmanthus (Osmanthus fragrans) cultivars by GC-olfactometry and GC-MS
Article Snippet: The n -alkane standards (C8 –C20 ), methyl laurate as internal standard, and the referenced authentic standards including trans -3-hexenol, β-myrcene, 4-hexen-1-ol, acetate, 3-carene, limonene, cis -β-ocimene, trans -β-ocimene, γ-terpinene, cis -linalool oxide, trans -linalool oxide, allo-ocimene, cis -3-hexenyl iso-butyrate, cis -3-hexenyl butanoate, hexyl butanoate, cis -3-hexenyl-2-methylbutanoate, cis- geraniol, linalyl formate, citral, cis -3-hexenyl hexanoate, cis -jasmone, α-ionone, dihydro-β-ionone, geranyl acetone, γ-decalactone, and trans -β-ionone, were obtained from Sigma Co., Ltd. (St. Louis, MO, USA).

Activity Assay:

Article Title: Genome-Wide Identification and Functional Analysis of Carboxylesterase and Methylesterase Gene Families in Peach (Prunus persica L. Batsch)
Article Snippet: .. Chemicals and Reagents The chemicals used for volatile identification and enzyme activity, including hexyl acetate, E -2-hexenyl acetate, Z -3- hexenyl acetate, hexanol, E -2-hexenol, Z -3-hexenol, 2-octanol, butyl acetate, geranyl acetate, ethyl hexanoate, ethyl benzoate, MeJA, MeSA, JA, and SA were purchased from Sigma-Aldrich (USA). .. Statistical Analysis Heatmap figures were generated by Multi Experiment Viewer (version 4.6.0).

Mass Spectrometry:

Article Title: Identification and Characterization of (3Z):(2E)-Hexenal Isomerases from Cucumber
Article Snippet: .. Mass spectra were generated by an Agilent 7200 accurate-mass quadrupole time-of-flight mass spectrometer, operating in electron ionization mode (70 eV) at 230°C and collected with an acquisition rate of 20 scans per second. volatiles were identified and quantified using standard solutions of Z -3-hexenal, Z -3-hexenol, E -2-hexenal, E -2-hexenol and (E,Z )-2,6-nonadienal (Sigma-Aldrich). (Z,Z )-3,6-nonadienal was synthesized from (Z,Z )-3,6-nonadienol (Ventos) as described below. .. Purification of (3Z ):(2E )-Hexenal Isomerase Activity from Cucumber Fruits All procedures (except for the FPLC runs at RT) were performed at 4°C.

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