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Creative PEGWorks heterobifunctional biotin peg3 4kd amine linker
Site-specific biotinylation of recombinant human thrombomodulin (A) Upon reaction between rTM-N3 and <t>triphenylphosphine-PEG3.4kD</t> -biotin, SDS PAGE reveals the presence of two species separated by approximately 4 kD (Lane 1), corresponding to the desired biotinylated conjugate (*) and unreacted rTM-N3 . A molecular weight shift was not observed in a parallel control reaction using rTM engineered without an azido group (Lane 2), demonstrating the specificity of the Staudinger ligation. (B) Western blot against human TM after initial conjugation (Lane 1) and subsequent purification (Lane 2). After purification via centrifugal dialysis and monomeric avidin chromatography, a single species corresponding to the expected molecular weight of the desired biotin-PEG-TM conjugate is observed (*). (C) Western blot against biotin using HRP-labeled streptavidin confirms biotinylation of the construct (*; Lane 1).
Heterobifunctional Biotin Peg3 4kd Amine Linker, supplied by Creative PEGWorks, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/heterobifunctional biotin peg3 4kd amine linker/product/Creative PEGWorks
Average 77 stars, based on 1 article reviews
Price from $9.99 to $1999.99
heterobifunctional biotin peg3 4kd amine linker - by Bioz Stars, 2019-12
77/100 stars

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1) Product Images from "Biomolecular Surface Engineering of Pancreatic Islets with Thrombomodulin"

Article Title: Biomolecular Surface Engineering of Pancreatic Islets with Thrombomodulin

Journal:

doi: 10.1016/j.actbio.2010.01.027

Site-specific biotinylation of recombinant human thrombomodulin (A) Upon reaction between rTM-N3 and triphenylphosphine-PEG3.4kD -biotin, SDS PAGE reveals the presence of two species separated by approximately 4 kD (Lane 1), corresponding to the desired biotinylated conjugate (*) and unreacted rTM-N3 . A molecular weight shift was not observed in a parallel control reaction using rTM engineered without an azido group (Lane 2), demonstrating the specificity of the Staudinger ligation. (B) Western blot against human TM after initial conjugation (Lane 1) and subsequent purification (Lane 2). After purification via centrifugal dialysis and monomeric avidin chromatography, a single species corresponding to the expected molecular weight of the desired biotin-PEG-TM conjugate is observed (*). (C) Western blot against biotin using HRP-labeled streptavidin confirms biotinylation of the construct (*; Lane 1).
Figure Legend Snippet: Site-specific biotinylation of recombinant human thrombomodulin (A) Upon reaction between rTM-N3 and triphenylphosphine-PEG3.4kD -biotin, SDS PAGE reveals the presence of two species separated by approximately 4 kD (Lane 1), corresponding to the desired biotinylated conjugate (*) and unreacted rTM-N3 . A molecular weight shift was not observed in a parallel control reaction using rTM engineered without an azido group (Lane 2), demonstrating the specificity of the Staudinger ligation. (B) Western blot against human TM after initial conjugation (Lane 1) and subsequent purification (Lane 2). After purification via centrifugal dialysis and monomeric avidin chromatography, a single species corresponding to the expected molecular weight of the desired biotin-PEG-TM conjugate is observed (*). (C) Western blot against biotin using HRP-labeled streptavidin confirms biotinylation of the construct (*; Lane 1).

Techniques Used: Recombinant, SDS Page, Molecular Weight, Ligation, Western Blot, Conjugation Assay, Purification, Avidin-Biotin Assay, Chromatography, Labeling, Construct

Site-specific biotinylation of recombinant human thrombomodulin (rTM) through Staudinger ligation rTM was engineered with a C-terminal azido group (1) and subsequently reacted with triphenylphosphine-PEG3.4kD -biotin (2) .
Figure Legend Snippet: Site-specific biotinylation of recombinant human thrombomodulin (rTM) through Staudinger ligation rTM was engineered with a C-terminal azido group (1) and subsequently reacted with triphenylphosphine-PEG3.4kD -biotin (2) .

Techniques Used: Recombinant, Ligation

Related Articles

Nuclear Magnetic Resonance:

Article Title: Biomolecular Surface Engineering of Pancreatic Islets with Thrombomodulin
Article Snippet: A triphenylphosphine-poly(ethylene glycol)-biotin conjugate was synthesized by reaction of a heterobifunctional biotin-PEG3.4kD -amine linker (CreativePEGWorks, Winston Salem, NC) with a pentafluorophenyl (PFP) active ester of triphenylphosphine, synthesized as described previously [ , ]. .. A triphenylphosphine-poly(ethylene glycol)-biotin conjugate was synthesized by reaction of a heterobifunctional biotin-PEG3.4kD -amine linker (CreativePEGWorks, Winston Salem, NC) with a pentafluorophenyl (PFP) active ester of triphenylphosphine, synthesized as described previously [ , ].

Filtration:

Article Title: Biomolecular Surface Engineering of Pancreatic Islets with Thrombomodulin
Article Snippet: A triphenylphosphine-poly(ethylene glycol)-biotin conjugate was synthesized by reaction of a heterobifunctional biotin-PEG3.4kD -amine linker (CreativePEGWorks, Winston Salem, NC) with a pentafluorophenyl (PFP) active ester of triphenylphosphine, synthesized as described previously [ , ]. .. A triphenylphosphine-poly(ethylene glycol)-biotin conjugate was synthesized by reaction of a heterobifunctional biotin-PEG3.4kD -amine linker (CreativePEGWorks, Winston Salem, NC) with a pentafluorophenyl (PFP) active ester of triphenylphosphine, synthesized as described previously [ , ].

Synthesized:

Article Title: Biomolecular Surface Engineering of Pancreatic Islets with Thrombomodulin
Article Snippet: Control rTM-methione was expressed using the same E. coli methionine auxotroph in Luria Bertani (LB) media. rTM was purified with immunoaffinity chromatography using anti-FLAG affinity gel (Sigma Aldrich). .. A triphenylphosphine-poly(ethylene glycol)-biotin conjugate was synthesized by reaction of a heterobifunctional biotin-PEG3.4kD -amine linker (CreativePEGWorks, Winston Salem, NC) with a pentafluorophenyl (PFP) active ester of triphenylphosphine, synthesized as described previously [ , ]. .. To a stirred solution of biotin-PEG3.4kD -amine (100 mg, 0.029 mmol) in dichloromethane (DCM; 2 mL) was added the PFP-ester of triphenylphosphine (31.17 mg, 0.058 mmol, 2 equiv) and Et3 N (8.08 µl, 2 equiv.), and the resultant mixture stirred at room temperature for 12 to 16 hr, upon which time volatiles were evaporated by vacuum.

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    Creative PEGWorks heterobifunctional biotin peg3 4kd amine linker
    Site-specific biotinylation of recombinant human thrombomodulin (A) Upon reaction between rTM-N3 and <t>triphenylphosphine-PEG3.4kD</t> -biotin, SDS PAGE reveals the presence of two species separated by approximately 4 kD (Lane 1), corresponding to the desired biotinylated conjugate (*) and unreacted rTM-N3 . A molecular weight shift was not observed in a parallel control reaction using rTM engineered without an azido group (Lane 2), demonstrating the specificity of the Staudinger ligation. (B) Western blot against human TM after initial conjugation (Lane 1) and subsequent purification (Lane 2). After purification via centrifugal dialysis and monomeric avidin chromatography, a single species corresponding to the expected molecular weight of the desired biotin-PEG-TM conjugate is observed (*). (C) Western blot against biotin using HRP-labeled streptavidin confirms biotinylation of the construct (*; Lane 1).
    Heterobifunctional Biotin Peg3 4kd Amine Linker, supplied by Creative PEGWorks, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heterobifunctional biotin peg3 4kd amine linker/product/Creative PEGWorks
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    heterobifunctional biotin peg3 4kd amine linker - by Bioz Stars, 2019-12
    77/100 stars
      Buy from Supplier

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    Site-specific biotinylation of recombinant human thrombomodulin (A) Upon reaction between rTM-N3 and triphenylphosphine-PEG3.4kD -biotin, SDS PAGE reveals the presence of two species separated by approximately 4 kD (Lane 1), corresponding to the desired biotinylated conjugate (*) and unreacted rTM-N3 . A molecular weight shift was not observed in a parallel control reaction using rTM engineered without an azido group (Lane 2), demonstrating the specificity of the Staudinger ligation. (B) Western blot against human TM after initial conjugation (Lane 1) and subsequent purification (Lane 2). After purification via centrifugal dialysis and monomeric avidin chromatography, a single species corresponding to the expected molecular weight of the desired biotin-PEG-TM conjugate is observed (*). (C) Western blot against biotin using HRP-labeled streptavidin confirms biotinylation of the construct (*; Lane 1).

    Journal:

    Article Title: Biomolecular Surface Engineering of Pancreatic Islets with Thrombomodulin

    doi: 10.1016/j.actbio.2010.01.027

    Figure Lengend Snippet: Site-specific biotinylation of recombinant human thrombomodulin (A) Upon reaction between rTM-N3 and triphenylphosphine-PEG3.4kD -biotin, SDS PAGE reveals the presence of two species separated by approximately 4 kD (Lane 1), corresponding to the desired biotinylated conjugate (*) and unreacted rTM-N3 . A molecular weight shift was not observed in a parallel control reaction using rTM engineered without an azido group (Lane 2), demonstrating the specificity of the Staudinger ligation. (B) Western blot against human TM after initial conjugation (Lane 1) and subsequent purification (Lane 2). After purification via centrifugal dialysis and monomeric avidin chromatography, a single species corresponding to the expected molecular weight of the desired biotin-PEG-TM conjugate is observed (*). (C) Western blot against biotin using HRP-labeled streptavidin confirms biotinylation of the construct (*; Lane 1).

    Article Snippet: A triphenylphosphine-poly(ethylene glycol)-biotin conjugate was synthesized by reaction of a heterobifunctional biotin-PEG3.4kD -amine linker (CreativePEGWorks, Winston Salem, NC) with a pentafluorophenyl (PFP) active ester of triphenylphosphine, synthesized as described previously [ , ].

    Techniques: Recombinant, SDS Page, Molecular Weight, Ligation, Western Blot, Conjugation Assay, Purification, Avidin-Biotin Assay, Chromatography, Labeling, Construct

    Site-specific biotinylation of recombinant human thrombomodulin (rTM) through Staudinger ligation rTM was engineered with a C-terminal azido group (1) and subsequently reacted with triphenylphosphine-PEG3.4kD -biotin (2) .

    Journal:

    Article Title: Biomolecular Surface Engineering of Pancreatic Islets with Thrombomodulin

    doi: 10.1016/j.actbio.2010.01.027

    Figure Lengend Snippet: Site-specific biotinylation of recombinant human thrombomodulin (rTM) through Staudinger ligation rTM was engineered with a C-terminal azido group (1) and subsequently reacted with triphenylphosphine-PEG3.4kD -biotin (2) .

    Article Snippet: A triphenylphosphine-poly(ethylene glycol)-biotin conjugate was synthesized by reaction of a heterobifunctional biotin-PEG3.4kD -amine linker (CreativePEGWorks, Winston Salem, NC) with a pentafluorophenyl (PFP) active ester of triphenylphosphine, synthesized as described previously [ , ].

    Techniques: Recombinant, Ligation