heterobifunctional biotin peg3 4kd amine linker (Creative PEGWorks)
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Heterobifunctional Biotin Peg3 4kd Amine Linker, supplied by Creative PEGWorks, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/heterobifunctional biotin peg3 4kd amine linker/product/Creative PEGWorks
Average 77 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Biomolecular Surface Engineering of Pancreatic Islets with Thrombomodulin"
Article Title: Biomolecular Surface Engineering of Pancreatic Islets with Thrombomodulin
Journal:
doi: 10.1016/j.actbio.2010.01.027

Figure Legend Snippet: Site-specific biotinylation of recombinant human thrombomodulin (A) Upon reaction between rTM-N3 and triphenylphosphine-PEG3.4kD -biotin, SDS PAGE reveals the presence of two species separated by approximately 4 kD (Lane 1), corresponding to the desired biotinylated conjugate (*) and unreacted rTM-N3 . A molecular weight shift was not observed in a parallel control reaction using rTM engineered without an azido group (Lane 2), demonstrating the specificity of the Staudinger ligation. (B) Western blot against human TM after initial conjugation (Lane 1) and subsequent purification (Lane 2). After purification via centrifugal dialysis and monomeric avidin chromatography, a single species corresponding to the expected molecular weight of the desired biotin-PEG-TM conjugate is observed (*). (C) Western blot against biotin using HRP-labeled streptavidin confirms biotinylation of the construct (*; Lane 1).
Techniques Used: Recombinant, SDS Page, Molecular Weight, Ligation, Western Blot, Conjugation Assay, Purification, Avidin-Biotin Assay, Chromatography, Labeling, Construct

Figure Legend Snippet: Site-specific biotinylation of recombinant human thrombomodulin (rTM) through Staudinger ligation rTM was engineered with a C-terminal azido group (1) and subsequently reacted with triphenylphosphine-PEG3.4kD -biotin (2) .
Techniques Used: Recombinant, Ligation
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