dulbecco  (ATCC)

Journal: PLoS ONE

Article Title: Distribution and Phylogeny of EFL and EF-1α in Euglenozoa Suggest Ancestral Co-Occurrence Followed by Differential Loss

doi: 10.1371/journal.pone.0005162

Figure Lengend Snippet: New sequences obtained in this study.

Article Snippet: Herpetomonas muscarum ATCC 30260 , EF-1α , PCR.

Techniques:

Journal: PLoS ONE

Article Title: Identification and Phylogenetic Analysis of Heme Synthesis Genes in Trypanosomatids and Their Bacterial Endosymbionts

doi: 10.1371/journal.pone.0023518

Figure Lengend Snippet: Heme biosynthesis enzyme presence in the endosymbiotic bacteria and the Kinetoplastida analyzed in this work.

Article Snippet: The genomes of the following organisms were sequenced: Parabodo caudatus ATCC 30905, Angomonas deanei (TCC036E), Angomonas desouzai (TCC079E), Strigomonas culicis (TCC012E), Strigomonas galati (TCC219), Strigomonas oncopelti (TCC290E), Herpetomonas muscarum (TCC001E), Crithidia acanthocephali (TCC037E), Endotrypanum schaudinni (TCC224), Leptomonas costaricensis (TCC169E), and Phytomonas sp. from Jatropha macrantha (TCC066E).

Techniques:

Journal: Parasitology

Article Title: Diverse telomeres in trypanosomatids

doi: 10.1017/S0031182021000378

Figure Lengend Snippet: Southern blotting analysis of telomere repeats in selected species of Trypanosomatidae. Marker sizes are indicated on the left. The vertical lines denote a composite image from the same blot. DNA integrity controls are presented in Supplementary Fig. 1 (left and middle panels).

Article Snippet: Cultures of Angomonas deanei (CT-IOC 044), A. desouzai (CT-IOC 109), Blastocrithidia sp. (p57), Blastocrithidia triatomae , Blechomonas ayalai (B08-376), Blechomonas pulexsimulantis (ATCC50186), Herpetomonas muscarum (MMO-01), Jaenimonas sp. (Finn-01.02), Kentomonas sorsogonicus (MF-08.02), Lafontella sp. (GMO-01), Vickermania ingenoplastis (CP21), Wallacemonas collosoma (ATCC30261) and W. rigidus (PL11) were maintained in SDM medium (BioWest) supplemented with 10% fetal bovine serum (BioWest) and 50 units mL −1 of Penicillin/Streptomycin (BioSera) at 23°C.

Techniques: Southern Blot, Marker

Journal: Parasitology

Article Title: Diverse telomeres in trypanosomatids

doi: 10.1017/S0031182021000378

Figure Lengend Snippet: Telomere lengths (weighted median, minimum–maximum) in selected species of Trypanosomatidae

Article Snippet: Cultures of Angomonas deanei (CT-IOC 044), A. desouzai (CT-IOC 109), Blastocrithidia sp. (p57), Blastocrithidia triatomae , Blechomonas ayalai (B08-376), Blechomonas pulexsimulantis (ATCC50186), Herpetomonas muscarum (MMO-01), Jaenimonas sp. (Finn-01.02), Kentomonas sorsogonicus (MF-08.02), Lafontella sp. (GMO-01), Vickermania ingenoplastis (CP21), Wallacemonas collosoma (ATCC30261) and W. rigidus (PL11) were maintained in SDM medium (BioWest) supplemented with 10% fetal bovine serum (BioWest) and 50 units mL −1 of Penicillin/Streptomycin (BioSera) at 23°C.

Techniques:

Journal: Parasites & Vectors

Article Title: The Phlebotomus papatasi systemic transcriptional response to trypanosomatid-contaminated blood does not differ from the non-infected blood meal

doi: 10.1186/s13071-020-04498-0

Figure Lengend Snippet: Principal component analysis showing that time was the major source of variation in the experiments with the trypanosomatids Herpetomonas muscarum , Leishmania donovani and L. major —and not infection status (condition). PC1 , PC2 Principal components 1 and 2, respectively

Article Snippet: Leishmania donovani (MHOM/ET/2010/GR374), L. major LV561 (LRC-L137; MHOM/IL/1967/Jericho-II) and Herpetomonas muscarum [ ] were cultured in M199 medium (Sigma-Aldrich, St. Louis, MO, USA) containing 10% heat-inactivated foetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1% Basal Medium Eagle vitamins (Sigma-Aldrich), 2% sterile urine, 250 μg/ml amikacin (Amikin; Bristol-Myers Squibb, Princeton Pike, NJ, USA) at 23 °C ( L. donovani , L. major ) or 28 °C ( H. muscarum ).

Techniques: Infection

Journal: Parasites & Vectors

Article Title: The Phlebotomus papatasi systemic transcriptional response to trypanosomatid-contaminated blood does not differ from the non-infected blood meal

doi: 10.1186/s13071-020-04498-0

Figure Lengend Snippet: Volcano plots of statistical significance against log2-fold changes in transcript abundance. Blood-fed Phlebotomus papatasi sand flies were compared to P. papatasi fed L. major ( a – c ), H. muscarum ( d – f ) and L. donovani ( g – i ). Dashed lines indicate the log2 fold change = − 2/2 and P = 0.05 thresholds. Transcripts in red dots exceed the statistical significance threshold. Green dots indicate transcripts which exceeded the fold-change thresholds but were not statistically significantly different between the two feeding conditions. Gray dots indicate transcripts which meet neither of the statistical or fold-change thresholds. Analysis was performed in DESeq2 package for R and visualized using the EnhancedVolcano function

Article Snippet: Leishmania donovani (MHOM/ET/2010/GR374), L. major LV561 (LRC-L137; MHOM/IL/1967/Jericho-II) and Herpetomonas muscarum [ ] were cultured in M199 medium (Sigma-Aldrich, St. Louis, MO, USA) containing 10% heat-inactivated foetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1% Basal Medium Eagle vitamins (Sigma-Aldrich), 2% sterile urine, 250 μg/ml amikacin (Amikin; Bristol-Myers Squibb, Princeton Pike, NJ, USA) at 23 °C ( L. donovani , L. major ) or 28 °C ( H. muscarum ).

Techniques:

Journal: Parasites & Vectors

Article Title: The Phlebotomus papatasi systemic transcriptional response to trypanosomatid-contaminated blood does not differ from the non-infected blood meal

doi: 10.1186/s13071-020-04498-0

Figure Lengend Snippet: Number of transcripts showing no significant change in expression by twofold or more in either direction between the blood-fed and trypanosomatid-fed sand fly Plebotomus papatasi

Article Snippet: Leishmania donovani (MHOM/ET/2010/GR374), L. major LV561 (LRC-L137; MHOM/IL/1967/Jericho-II) and Herpetomonas muscarum [ ] were cultured in M199 medium (Sigma-Aldrich, St. Louis, MO, USA) containing 10% heat-inactivated foetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1% Basal Medium Eagle vitamins (Sigma-Aldrich), 2% sterile urine, 250 μg/ml amikacin (Amikin; Bristol-Myers Squibb, Princeton Pike, NJ, USA) at 23 °C ( L. donovani , L. major ) or 28 °C ( H. muscarum ).

Techniques: Expressing