Structured Review

Fisher Scientific hepes
binding of ca 2+ ions is determined by membrane charge. titration curves of bound ca 2+ versus added ca 2+ for ca 2+ binding to lipid vesicles composed of (black) 100% dopc, (blue) 55:45 mol% dopc/dops, or (red) 35:30:30:5 mol% dopc/dope/dops/pi(4,5)p 2 . all titrations were performed in 37.5 mm <t>nacl</t> and 7 mm <t>hepes</t> at ph 7, to match saline concentrations used in guv experiments. error bars in the y axis are from the propagation of error in the ise measurement, while x axis error is from the propagation of error from the lipid (determined by phosphate assay) and cacl 2 .
Hepes, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 99/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Cations induce shape remodeling of negatively charged phospholipid membranes"

Article Title: Cations induce shape remodeling of negatively charged phospholipid membranes

Journal: Physical chemistry chemical physics : PCCP

doi: 10.1039/c7cp00718c

binding of ca 2+ ions is determined by membrane charge. titration curves of bound ca 2+ versus added ca 2+ for ca 2+ binding to lipid vesicles composed of (black) 100% dopc, (blue) 55:45 mol% dopc/dops, or (red) 35:30:30:5 mol% dopc/dope/dops/pi(4,5)p 2 . all titrations were performed in 37.5 mm nacl and 7 mm hepes at ph 7, to match saline concentrations used in guv experiments. error bars in the y axis are from the propagation of error in the ise measurement, while x axis error is from the propagation of error from the lipid (determined by phosphate assay) and cacl 2 .
Figure Legend Snippet: binding of ca 2+ ions is determined by membrane charge. titration curves of bound ca 2+ versus added ca 2+ for ca 2+ binding to lipid vesicles composed of (black) 100% dopc, (blue) 55:45 mol% dopc/dops, or (red) 35:30:30:5 mol% dopc/dope/dops/pi(4,5)p 2 . all titrations were performed in 37.5 mm nacl and 7 mm hepes at ph 7, to match saline concentrations used in guv experiments. error bars in the y axis are from the propagation of error in the ise measurement, while x axis error is from the propagation of error from the lipid (determined by phosphate assay) and cacl 2 .

Techniques Used: Binding Assay, Titration

2) Product Images from "Anisotropic nanocrystal arrays organized on protein lattices formed by recombinant clathrin fragments"

Article Title: Anisotropic nanocrystal arrays organized on protein lattices formed by recombinant clathrin fragments

Journal: Journal of materials chemistry

doi: 10.1039/C2JM35019J

Cage-like assemblies of Hub-His 6 and Hub-NoTag. pH-dependent assembly and disassembly of (a) Hub-His 6 and (b) Hub-NoTag, monitored by light scattering at 320 nm. Each protein preparation (0.1 mg/mL,100 µL) was assembled by the addition of 10 µL of 1.5 M HEPES buffer (pH 6.5) at the respective times (as indicated by the white arrows); disassembly was triggered with the addition of 11µL of 1.0 M Tris buffer (pH 9.0) at the respective times (black arrows). Stained TEM images of (c) Hub-His 6 and (d) Hub-NoTag were obtained. Scale bar represents 200 nm.
Figure Legend Snippet: Cage-like assemblies of Hub-His 6 and Hub-NoTag. pH-dependent assembly and disassembly of (a) Hub-His 6 and (b) Hub-NoTag, monitored by light scattering at 320 nm. Each protein preparation (0.1 mg/mL,100 µL) was assembled by the addition of 10 µL of 1.5 M HEPES buffer (pH 6.5) at the respective times (as indicated by the white arrows); disassembly was triggered with the addition of 11µL of 1.0 M Tris buffer (pH 9.0) at the respective times (black arrows). Stained TEM images of (c) Hub-His 6 and (d) Hub-NoTag were obtained. Scale bar represents 200 nm.

Techniques Used: Staining, Transmission Electron Microscopy

3) Product Images from "Protected Graft Copolymer Excipient Leads to a Higher Acute Maximum Tolerated Dose and Extends Residence Time of Vasoactive Intestinal Peptide Significantly Better than Sterically Stabilized Micelles"

Article Title: Protected Graft Copolymer Excipient Leads to a Higher Acute Maximum Tolerated Dose and Extends Residence Time of Vasoactive Intestinal Peptide Significantly Better than Sterically Stabilized Micelles

Journal: Pharmaceutical research

doi: 10.1007/s11095-012-0904-4

VIP binding in 100 mM NaCl, 100 mM HEPES at a carrier concentration of 2.4 mg/ml for PGC and 2.0 mg/ml for SSM ( a ) PGC-VIP, ( b ) SSM-VIP. The experiment was done in quadruplicate; free VIP was separated from bound by ultrafiltration, VIP quantitation was
Figure Legend Snippet: VIP binding in 100 mM NaCl, 100 mM HEPES at a carrier concentration of 2.4 mg/ml for PGC and 2.0 mg/ml for SSM ( a ) PGC-VIP, ( b ) SSM-VIP. The experiment was done in quadruplicate; free VIP was separated from bound by ultrafiltration, VIP quantitation was

Techniques Used: Binding Assay, Concentration Assay, Pyrolysis Gas Chromatography, Quantitation Assay

Related Articles

Centrifugation:

Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
Article Snippet: Proteomics Analyses Samples of whole cells and culture supernatants of wild-type, Δess , and complemented GAS M1 5448 were collected by centrifugation of overnight cultures (10 mL) for 10 minutes at 10,000 × g at 4°C. .. Bacterial cell pellets were suspended in 500 μL of lysis buffer composed of 75 mM NaCl, 3% sodium dodecyl sulfate (SDS) (Fisher Scientific), 1 mM sodium Fluoride (VWR International, LLC), 1 mM beta-glycerophosphate (Sigma Aldrich), 1 mM sodium orthovanadate, 10 mM sodium pyrophosphate (VWR International, LLC), 1 mM phenylmethylsulfonyl fluoride (Fisher Scientific), 50 mM HEPES (Fisher Scientific) pH 8.5, and 1X cOmplete EDTA-free protease inhibitor cocktail, plus 500 μL of 8M Urea (Fisher Scientific), 50 mM HEPES pH 8.5, and subjected to sonication using a Q500 QSonica sonicator (Qsonica) equipped with 1.6 mm microtip at amplitude 20%.

Article Title: S-nitrosylation of endogenous protein tyrosine phosphatases in endothelial insulin signaling
Article Snippet: Cells were harvested in lysis buffer containing 25 mM HEPES (pH 7.4, BP310-1 from Thermal Fisher Scientific), 150 mM NaCl (BP358-10 from Thermal Fisher Scientific), 1 mM EDTA (disodium salt dihydrate, adjusted to pH 8, S311-500 from Thermal Fisher Scientific), 1% NP-40 (19628 from USB) and protease inhibitors (04-693-132-001 from Roche). .. An aliquot of total lysates (800 μg) was reacted with 50 mM IAM (M216-30G from Amresco) in lysis buffer containing 2.5% SDS (L5750 from Sigma) at room temperature (RT) for 30 min. Proteins were precipitated by cold acetone (100%, 179,124-4L from Sigma) and collected by centrifugation at 2000xg for 5 min, subsequently washed with cold acetone (70%) 3 times.

Transferring:

Article Title: Tracking Endocytosis and Intracellular Trafficking of Epitope-tagged Syntaxin 3 by Antibody Feeding in Live, Polarized MDCK Cells
Article Snippet: 10 cm cell culture dishes (Corning, Falcon® , catalog number: 353003) 10 ml serological pipette (VWR, catalog number: 89130-898) 50 ml conical tubes (Corning, catalog number: 430290) 12-well cell culture plates with 0.4 μm pore polycarbonate Transwell supports (Corning, catalog number: 3401) 12-well cell culture plates (Corning, Costar® , catalog number: 3512) Razor blades (VWR, catalog number: 55411-050) Paper towels Kimwipes (KCWW, Kimberly-Clark, catalog number: 34120) Parafilm (BEMIS, catalog number: PM996) Plastic pencil box with lid (VWR, catalog number: 500003-109) Manufacturer: Janitorial Supplies, catalog number: AVT34104. .. Sterile 1× Dulbecco’s phosphate buffered saline (DPBS) without calcium or magnesium (Mediatech, catalog number: 21-031-CV) Sterile 0.25% trypsin/EDTA (Mediatech, catalog number: 22-053-CI) 4% buffered formalin solution (Sigma-Aldrich, catalog number: HT5012) Prolong Gold anti-fade reagent plus DAPI (Thermo Fisher Scientific, Invitrogen™, catalog number: ) Sterile Minimum Essential Medium without glutamine (Mediatech, catalog number: 15-010-CV) Sterile L-glutamine 100× (Mediatech, catalog number: 25-005-CI) Penicillin/Streptomycin 100× (Mediatech, catalog number: 30-002-CI) Fetal bovine serum (FBS) (Omega Scientific, catalog number: FB-11) Doxycycline monohydrate (tetracycline analog) (Sigma-Aldrich, catalog number: D1822) HEPES (Fisher Scientific, catalog number: BP310) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A2153) Anti-c-Myc monoclonal antibody, clone 9E10 Note: Hybridoma cells from The Developmental Studies Hybridoma Bank ( ).

Article Title: Electrical Communication in Lymphangions
Article Snippet: The patch pipette solution contained the following (in mM): 135 KCl, 10 HEPES, 10 ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid, and 5 MgGTP (pH adjusted to 7.2 with CsOH). .. All chemicals were obtained from Sigma except for BSA (US Biochemicals, Cleveland, OH), MgSO4 , and HEPES (Fisher Scientific, Pittsburgh, PA).

Mass Spectrometry:

Article Title: A Novel Postsynaptic Mechanism for Heterosynaptic Sharing of Short-Term Plasticity
Article Snippet: Baseline EPSP amplitudes were determined by injection of current to elicit a single test spike in the SN in each of the two presynaptic SNs (~200 ms apart). .. 200 mM BAPTA (tetrapotassium salt, Molecular Probes, Eugene, OR), 25 μM heparin (Calbiochem, San Diego, CA), and 125 μM IP3 were dissolved in 3 M KCl, 10 mM HEPES, and 3 mM Fast Green dye (Fisher Scientific).

Stable Transfection:

Article Title: Tracking Endocytosis and Intracellular Trafficking of Epitope-tagged Syntaxin 3 by Antibody Feeding in Live, Polarized MDCK Cells
Article Snippet: The parental cell line, expressing the TET transactivator is required to produce doxycycline-inducible, stably transfected cells for one’s gene of interest and have been generated in the laboratory of the senior author (TW). .. Sterile 1× Dulbecco’s phosphate buffered saline (DPBS) without calcium or magnesium (Mediatech, catalog number: 21-031-CV) Sterile 0.25% trypsin/EDTA (Mediatech, catalog number: 22-053-CI) 4% buffered formalin solution (Sigma-Aldrich, catalog number: HT5012) Prolong Gold anti-fade reagent plus DAPI (Thermo Fisher Scientific, Invitrogen™, catalog number: ) Sterile Minimum Essential Medium without glutamine (Mediatech, catalog number: 15-010-CV) Sterile L-glutamine 100× (Mediatech, catalog number: 25-005-CI) Penicillin/Streptomycin 100× (Mediatech, catalog number: 30-002-CI) Fetal bovine serum (FBS) (Omega Scientific, catalog number: FB-11) Doxycycline monohydrate (tetracycline analog) (Sigma-Aldrich, catalog number: D1822) HEPES (Fisher Scientific, catalog number: BP310) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A2153) Anti-c-Myc monoclonal antibody, clone 9E10 Note: Hybridoma cells from The Developmental Studies Hybridoma Bank ( ).

Pyrolysis Gas Chromatography:

Article Title: Protected Graft Copolymer Excipient Leads to a Higher Acute Maximum Tolerated Dose and Extends Residence Time of Vasoactive Intestinal Peptide Significantly Better than Sterically Stabilized Micelles
Article Snippet: .. VIP was mixed with a fixed amount of PGC (0.6 mg at 2.4 mg/ml final concentration) or SSM carriers (0.5 mg at 2 mg/ml final concentration) at concentrations between 72 μM and 188 μM in the PGC mixtures and between 120 μM and 240 μM in the SSM mixtures in an aqueous solution of 100 mM NaCl (Fisher Scientific, Waltham MA) and 100 mM HEPES (Fisher Scientific, Waltham MA) in quadruplicate. .. The mixtures were subjected to ultrafiltration using 100 kDa MWCO cellulose membrane centrifuge filter (Amicon Ultra Ultracel 100 k, Millipore, Billerica MA) by centrifuging at 18,000×g for 12 min.

Expressing:

Article Title: Tracking Endocytosis and Intracellular Trafficking of Epitope-tagged Syntaxin 3 by Antibody Feeding in Live, Polarized MDCK Cells
Article Snippet: The parental cell line, expressing the TET transactivator is required to produce doxycycline-inducible, stably transfected cells for one’s gene of interest and have been generated in the laboratory of the senior author (TW). .. Sterile 1× Dulbecco’s phosphate buffered saline (DPBS) without calcium or magnesium (Mediatech, catalog number: 21-031-CV) Sterile 0.25% trypsin/EDTA (Mediatech, catalog number: 22-053-CI) 4% buffered formalin solution (Sigma-Aldrich, catalog number: HT5012) Prolong Gold anti-fade reagent plus DAPI (Thermo Fisher Scientific, Invitrogen™, catalog number: ) Sterile Minimum Essential Medium without glutamine (Mediatech, catalog number: 15-010-CV) Sterile L-glutamine 100× (Mediatech, catalog number: 25-005-CI) Penicillin/Streptomycin 100× (Mediatech, catalog number: 30-002-CI) Fetal bovine serum (FBS) (Omega Scientific, catalog number: FB-11) Doxycycline monohydrate (tetracycline analog) (Sigma-Aldrich, catalog number: D1822) HEPES (Fisher Scientific, catalog number: BP310) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A2153) Anti-c-Myc monoclonal antibody, clone 9E10 Note: Hybridoma cells from The Developmental Studies Hybridoma Bank ( ).

High Performance Liquid Chromatography:

Article Title: Protected Graft Copolymer Excipient Leads to a Higher Acute Maximum Tolerated Dose and Extends Residence Time of Vasoactive Intestinal Peptide Significantly Better than Sterically Stabilized Micelles
Article Snippet: VIP was mixed with a fixed amount of PGC (0.6 mg at 2.4 mg/ml final concentration) or SSM carriers (0.5 mg at 2 mg/ml final concentration) at concentrations between 72 μM and 188 μM in the PGC mixtures and between 120 μM and 240 μM in the SSM mixtures in an aqueous solution of 100 mM NaCl (Fisher Scientific, Waltham MA) and 100 mM HEPES (Fisher Scientific, Waltham MA) in quadruplicate. .. VIP was mixed with a fixed amount of PGC (0.6 mg at 2.4 mg/ml final concentration) or SSM carriers (0.5 mg at 2 mg/ml final concentration) at concentrations between 72 μM and 188 μM in the PGC mixtures and between 120 μM and 240 μM in the SSM mixtures in an aqueous solution of 100 mM NaCl (Fisher Scientific, Waltham MA) and 100 mM HEPES (Fisher Scientific, Waltham MA) in quadruplicate.

Transfection:

Article Title: Tracking Endocytosis and Intracellular Trafficking of Epitope-tagged Syntaxin 3 by Antibody Feeding in Live, Polarized MDCK Cells
Article Snippet: The parental cell line, expressing the TET transactivator is required to produce doxycycline-inducible, stably transfected cells for one’s gene of interest and have been generated in the laboratory of the senior author (TW). .. Sterile 1× Dulbecco’s phosphate buffered saline (DPBS) without calcium or magnesium (Mediatech, catalog number: 21-031-CV) Sterile 0.25% trypsin/EDTA (Mediatech, catalog number: 22-053-CI) 4% buffered formalin solution (Sigma-Aldrich, catalog number: HT5012) Prolong Gold anti-fade reagent plus DAPI (Thermo Fisher Scientific, Invitrogen™, catalog number: ) Sterile Minimum Essential Medium without glutamine (Mediatech, catalog number: 15-010-CV) Sterile L-glutamine 100× (Mediatech, catalog number: 25-005-CI) Penicillin/Streptomycin 100× (Mediatech, catalog number: 30-002-CI) Fetal bovine serum (FBS) (Omega Scientific, catalog number: FB-11) Doxycycline monohydrate (tetracycline analog) (Sigma-Aldrich, catalog number: D1822) HEPES (Fisher Scientific, catalog number: BP310) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A2153) Anti-c-Myc monoclonal antibody, clone 9E10 Note: Hybridoma cells from The Developmental Studies Hybridoma Bank ( ).

Concentration Assay:

Article Title: Cations induce shape remodeling of negatively charged phospholipid membranes
Article Snippet: The concentration of lipid stock solutions was periodically verified by phosphate assay. .. CaCl2 , MgCl2 , NaCl, Casein, Tris, HEPES, DTT and EDTA were obtained from Fisher Scientific (Rochester, NY).

Article Title: S-nitrosylation of endogenous protein tyrosine phosphatases in endothelial insulin signaling
Article Snippet: Cells were harvested in lysis buffer containing 25 mM HEPES (pH 7.4, BP310-1 from Thermal Fisher Scientific), 150 mM NaCl (BP358-10 from Thermal Fisher Scientific), 1 mM EDTA (disodium salt dihydrate, adjusted to pH 8, S311-500 from Thermal Fisher Scientific), 1% NP-40 (19628 from USB) and protease inhibitors (04-693-132-001 from Roche). .. The concentration of SDS in the processed lysates was diluted by PBS to 0.2%.

Article Title: Inhibition of plasminogen activation by apo(a): role of carboxyl-terminal lysines and identification of inhibitory domains in apo(a) [S]
Article Snippet: Human acute monocytic leukemia (THP-1) cells were grown in RPMI complete growth medium 1640 (GIBCO) adjusted to contain 4.5 g/l glucose (Sigma-Aldrich), 10 mM HEPES (Fisher Scientific), 1.0 mM sodium pyruvate (GIBCO), and 0.05 mM 2-mercaptoethanol (Sigma-Aldrich) and supplemented with 10% FBS (GIBCO) and 1% antibiotic-antimycotic (GIBCO). .. PMA (Sigma-Aldrich) was added to THP-1 cells at a final concentration of 0.1 μM for 72 h to differentiate them into macrophage-like cells.

Article Title: Protected Graft Copolymer Excipient Leads to a Higher Acute Maximum Tolerated Dose and Extends Residence Time of Vasoactive Intestinal Peptide Significantly Better than Sterically Stabilized Micelles
Article Snippet: .. VIP was mixed with a fixed amount of PGC (0.6 mg at 2.4 mg/ml final concentration) or SSM carriers (0.5 mg at 2 mg/ml final concentration) at concentrations between 72 μM and 188 μM in the PGC mixtures and between 120 μM and 240 μM in the SSM mixtures in an aqueous solution of 100 mM NaCl (Fisher Scientific, Waltham MA) and 100 mM HEPES (Fisher Scientific, Waltham MA) in quadruplicate. .. The mixtures were subjected to ultrafiltration using 100 kDa MWCO cellulose membrane centrifuge filter (Amicon Ultra Ultracel 100 k, Millipore, Billerica MA) by centrifuging at 18,000×g for 12 min.

Protease Inhibitor:

Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
Article Snippet: .. Bacterial cell pellets were suspended in 500 μL of lysis buffer composed of 75 mM NaCl, 3% sodium dodecyl sulfate (SDS) (Fisher Scientific), 1 mM sodium Fluoride (VWR International, LLC), 1 mM beta-glycerophosphate (Sigma Aldrich), 1 mM sodium orthovanadate, 10 mM sodium pyrophosphate (VWR International, LLC), 1 mM phenylmethylsulfonyl fluoride (Fisher Scientific), 50 mM HEPES (Fisher Scientific) pH 8.5, and 1X cOmplete EDTA-free protease inhibitor cocktail, plus 500 μL of 8M Urea (Fisher Scientific), 50 mM HEPES pH 8.5, and subjected to sonication using a Q500 QSonica sonicator (Qsonica) equipped with 1.6 mm microtip at amplitude 20%. .. Samples were subjected to 10 s of sonication followed by 10 s of rest, with a total sonication time of 30 s. For mouse spleens, 500 μL of lysis buffer and 500 μL of 8M Urea, 50 mM HEPES pH 8.5 was added to homogenized samples in PBS.

Dissection:

Article Title: Electrical Communication in Lymphangions
Article Snippet: Dissection and pipette solutions were Krebs plus the addition of purified 0.5% bovine serum albumin (BSA). .. All chemicals were obtained from Sigma except for BSA (US Biochemicals, Cleveland, OH), MgSO4 , and HEPES (Fisher Scientific, Pittsburgh, PA).

Cell Culture:

Article Title: Tracking Endocytosis and Intracellular Trafficking of Epitope-tagged Syntaxin 3 by Antibody Feeding in Live, Polarized MDCK Cells
Article Snippet: 10 cm cell culture dishes (Corning, Falcon® , catalog number: 353003) 10 ml serological pipette (VWR, catalog number: 89130-898) 50 ml conical tubes (Corning, catalog number: 430290) 12-well cell culture plates with 0.4 μm pore polycarbonate Transwell supports (Corning, catalog number: 3401) 12-well cell culture plates (Corning, Costar® , catalog number: 3512) Razor blades (VWR, catalog number: 55411-050) Paper towels Kimwipes (KCWW, Kimberly-Clark, catalog number: 34120) Parafilm (BEMIS, catalog number: PM996) Plastic pencil box with lid (VWR, catalog number: 500003-109) Manufacturer: Janitorial Supplies, catalog number: AVT34104. .. Sterile 1× Dulbecco’s phosphate buffered saline (DPBS) without calcium or magnesium (Mediatech, catalog number: 21-031-CV) Sterile 0.25% trypsin/EDTA (Mediatech, catalog number: 22-053-CI) 4% buffered formalin solution (Sigma-Aldrich, catalog number: HT5012) Prolong Gold anti-fade reagent plus DAPI (Thermo Fisher Scientific, Invitrogen™, catalog number: ) Sterile Minimum Essential Medium without glutamine (Mediatech, catalog number: 15-010-CV) Sterile L-glutamine 100× (Mediatech, catalog number: 25-005-CI) Penicillin/Streptomycin 100× (Mediatech, catalog number: 30-002-CI) Fetal bovine serum (FBS) (Omega Scientific, catalog number: FB-11) Doxycycline monohydrate (tetracycline analog) (Sigma-Aldrich, catalog number: D1822) HEPES (Fisher Scientific, catalog number: BP310) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A2153) Anti-c-Myc monoclonal antibody, clone 9E10 Note: Hybridoma cells from The Developmental Studies Hybridoma Bank ( ).

Article Title: 18F-FDG Is a Surrogate Marker of Therapy Response and Tumor Recovery after Drug Withdrawal during Treatment with a Dual PI3K/mTOR Inhibitor in a Preclinical Model of Cisplatin-Resistant Ovarian Cancer 1F-FDG Is a Surrogate Marker of Therapy Response and Tumor Recovery after Drug Withdrawal during Treatment with a Dual PI3K/mTOR Inhibitor in a Preclinical Model of Cisplatin-Resistant Ovarian Cance
Article Snippet: .. The SKOV3 cell line (American Type Cell Collection, Manassas, VA) was cultured in 450-cm2 flasks in RPMI 1640 medium supplemented with 2 mM Glutamax, 25 mM Hepes, 10% fetal calf serum, and 33 mM sodium bicarbonate (Fisher Scientific Bioblock, Illkirch, France) at 37°C in a 95% humidified air, 5% CO2 atmosphere. ..

Article Title: Inhibition of plasminogen activation by apo(a): role of carboxyl-terminal lysines and identification of inhibitory domains in apo(a) [S]
Article Snippet: Paragraph title: Cell culture ... Human acute monocytic leukemia (THP-1) cells were grown in RPMI complete growth medium 1640 (GIBCO) adjusted to contain 4.5 g/l glucose (Sigma-Aldrich), 10 mM HEPES (Fisher Scientific), 1.0 mM sodium pyruvate (GIBCO), and 0.05 mM 2-mercaptoethanol (Sigma-Aldrich) and supplemented with 10% FBS (GIBCO) and 1% antibiotic-antimycotic (GIBCO).

Generated:

Article Title: Tracking Endocytosis and Intracellular Trafficking of Epitope-tagged Syntaxin 3 by Antibody Feeding in Live, Polarized MDCK Cells
Article Snippet: The parental cell line, expressing the TET transactivator is required to produce doxycycline-inducible, stably transfected cells for one’s gene of interest and have been generated in the laboratory of the senior author (TW). .. Sterile 1× Dulbecco’s phosphate buffered saline (DPBS) without calcium or magnesium (Mediatech, catalog number: 21-031-CV) Sterile 0.25% trypsin/EDTA (Mediatech, catalog number: 22-053-CI) 4% buffered formalin solution (Sigma-Aldrich, catalog number: HT5012) Prolong Gold anti-fade reagent plus DAPI (Thermo Fisher Scientific, Invitrogen™, catalog number: ) Sterile Minimum Essential Medium without glutamine (Mediatech, catalog number: 15-010-CV) Sterile L-glutamine 100× (Mediatech, catalog number: 25-005-CI) Penicillin/Streptomycin 100× (Mediatech, catalog number: 30-002-CI) Fetal bovine serum (FBS) (Omega Scientific, catalog number: FB-11) Doxycycline monohydrate (tetracycline analog) (Sigma-Aldrich, catalog number: D1822) HEPES (Fisher Scientific, catalog number: BP310) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A2153) Anti-c-Myc monoclonal antibody, clone 9E10 Note: Hybridoma cells from The Developmental Studies Hybridoma Bank ( ).

Article Title: A Novel Postsynaptic Mechanism for Heterosynaptic Sharing of Short-Term Plasticity
Article Snippet: One minute later, a tetanizing stimulus (generated by individual current pulses [10 nA] at 20 or 7 Hz for 2 sec) was applied to the SNs. .. 200 mM BAPTA (tetrapotassium salt, Molecular Probes, Eugene, OR), 25 μM heparin (Calbiochem, San Diego, CA), and 125 μM IP3 were dissolved in 3 M KCl, 10 mM HEPES, and 3 mM Fast Green dye (Fisher Scientific).

other:

Article Title: Anisotropic nanocrystal arrays organized on protein lattices formed by recombinant clathrin fragments
Article Snippet: Imidazole, tris base, dithiothreitol (DTT), Isopropyl β-D-1-thiogalactopyranoside (IPTG), HEPES were purchased from Fisher Scientific. (CH3 )3 PAuCl, Thrombin CleanCleave™ Kit and uranyl acetate were purchased from Sigma Aldrich.

Article Title: PLASMA FROM PREECLAMPTIC WOMEN INCREASES BLOOD-BRAIN BARRIER PERMEABILITY: ROLE OF VEGF SIGNALING
Article Snippet: HEPES and indomethacin were purchased from Fisher Scientific (Hampton, NH); papaverine, L-NNA, and (calcium ionophore) were purchased from Sigma (St. Louis, MO).

Sonication:

Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
Article Snippet: .. Bacterial cell pellets were suspended in 500 μL of lysis buffer composed of 75 mM NaCl, 3% sodium dodecyl sulfate (SDS) (Fisher Scientific), 1 mM sodium Fluoride (VWR International, LLC), 1 mM beta-glycerophosphate (Sigma Aldrich), 1 mM sodium orthovanadate, 10 mM sodium pyrophosphate (VWR International, LLC), 1 mM phenylmethylsulfonyl fluoride (Fisher Scientific), 50 mM HEPES (Fisher Scientific) pH 8.5, and 1X cOmplete EDTA-free protease inhibitor cocktail, plus 500 μL of 8M Urea (Fisher Scientific), 50 mM HEPES pH 8.5, and subjected to sonication using a Q500 QSonica sonicator (Qsonica) equipped with 1.6 mm microtip at amplitude 20%. .. Samples were subjected to 10 s of sonication followed by 10 s of rest, with a total sonication time of 30 s. For mouse spleens, 500 μL of lysis buffer and 500 μL of 8M Urea, 50 mM HEPES pH 8.5 was added to homogenized samples in PBS.

Injection:

Article Title: A Novel Postsynaptic Mechanism for Heterosynaptic Sharing of Short-Term Plasticity
Article Snippet: Baseline EPSP amplitudes were determined by injection of current to elicit a single test spike in the SN in each of the two presynaptic SNs (~200 ms apart). .. 200 mM BAPTA (tetrapotassium salt, Molecular Probes, Eugene, OR), 25 μM heparin (Calbiochem, San Diego, CA), and 125 μM IP3 were dissolved in 3 M KCl, 10 mM HEPES, and 3 mM Fast Green dye (Fisher Scientific).

Binding Assay:

Article Title: Protected Graft Copolymer Excipient Leads to a Higher Acute Maximum Tolerated Dose and Extends Residence Time of Vasoactive Intestinal Peptide Significantly Better than Sterically Stabilized Micelles
Article Snippet: Paragraph title: Binding Studies and Determination of the Dissociation Constant (Kd) ... VIP was mixed with a fixed amount of PGC (0.6 mg at 2.4 mg/ml final concentration) or SSM carriers (0.5 mg at 2 mg/ml final concentration) at concentrations between 72 μM and 188 μM in the PGC mixtures and between 120 μM and 240 μM in the SSM mixtures in an aqueous solution of 100 mM NaCl (Fisher Scientific, Waltham MA) and 100 mM HEPES (Fisher Scientific, Waltham MA) in quadruplicate.

Isolation:

Article Title: Electrical Communication in Lymphangions
Article Snippet: Krebs buffer for isolated vessel protocols contained (in mM): 146.9 NaCl, 4.7 KCl, 2 CaCl2 ⋠H2 O, 1.2 MgSO4 , 1.2 NaH2 PO4 ⋠H2 O, 3 NaHCO3 , 1.5 NaHEPES, and 5 D-glucose (pH 7.4 at 37°C). .. All chemicals were obtained from Sigma except for BSA (US Biochemicals, Cleveland, OH), MgSO4 , and HEPES (Fisher Scientific, Pittsburgh, PA).

Size-exclusion Chromatography:

Article Title: A Novel Postsynaptic Mechanism for Heterosynaptic Sharing of Short-Term Plasticity
Article Snippet: A posttest EPSP was measured 10 sec after the offset of the stimulus train. .. 200 mM BAPTA (tetrapotassium salt, Molecular Probes, Eugene, OR), 25 μM heparin (Calbiochem, San Diego, CA), and 125 μM IP3 were dissolved in 3 M KCl, 10 mM HEPES, and 3 mM Fast Green dye (Fisher Scientific).

Microscopy:

Article Title: Tracking Endocytosis and Intracellular Trafficking of Epitope-tagged Syntaxin 3 by Antibody Feeding in Live, Polarized MDCK Cells
Article Snippet: Microscope slides (any standard slides from any supplier will be fine) Micro cover glasses, 18 × 18 mm, No. 1 thickness is 0.13 to 0.17 mm (VWR, catalog number: 48366-045) Madin-Darby Canine Kidney (MDCK) cells stably expressing C-terminally Myc2 -His6 -tagged Stx3 Note: Madin-Darby Canine Kidney (MDCK) cells stably expressing C-terminally Myc2 -His6 -tagged Stx3 using a doxycycline-inducible expression system have been described in detail in . .. Sterile 1× Dulbecco’s phosphate buffered saline (DPBS) without calcium or magnesium (Mediatech, catalog number: 21-031-CV) Sterile 0.25% trypsin/EDTA (Mediatech, catalog number: 22-053-CI) 4% buffered formalin solution (Sigma-Aldrich, catalog number: HT5012) Prolong Gold anti-fade reagent plus DAPI (Thermo Fisher Scientific, Invitrogen™, catalog number: ) Sterile Minimum Essential Medium without glutamine (Mediatech, catalog number: 15-010-CV) Sterile L-glutamine 100× (Mediatech, catalog number: 25-005-CI) Penicillin/Streptomycin 100× (Mediatech, catalog number: 30-002-CI) Fetal bovine serum (FBS) (Omega Scientific, catalog number: FB-11) Doxycycline monohydrate (tetracycline analog) (Sigma-Aldrich, catalog number: D1822) HEPES (Fisher Scientific, catalog number: BP310) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A2153) Anti-c-Myc monoclonal antibody, clone 9E10 Note: Hybridoma cells from The Developmental Studies Hybridoma Bank ( ).

Purification:

Article Title: Electrical Communication in Lymphangions
Article Snippet: Dissection and pipette solutions were Krebs plus the addition of purified 0.5% bovine serum albumin (BSA). .. All chemicals were obtained from Sigma except for BSA (US Biochemicals, Cleveland, OH), MgSO4 , and HEPES (Fisher Scientific, Pittsburgh, PA).

Patch Clamp:

Article Title: Electrical Communication in Lymphangions
Article Snippet: The bath solution for patch-clamp recordings contained the following (in mM): 1.8 mM CaCl2 , 110 NaCl, 1 CsCl, 1.2 MgCl2 , 10 HEPES, and 10 D-glucose (pH adjusted to 7.4 with NaOH). .. All chemicals were obtained from Sigma except for BSA (US Biochemicals, Cleveland, OH), MgSO4 , and HEPES (Fisher Scientific, Pittsburgh, PA).

Subculturing Assay:

Article Title: Inhibition of plasminogen activation by apo(a): role of carboxyl-terminal lysines and identification of inhibitory domains in apo(a) [S]
Article Snippet: Human acute monocytic leukemia (THP-1) cells were grown in RPMI complete growth medium 1640 (GIBCO) adjusted to contain 4.5 g/l glucose (Sigma-Aldrich), 10 mM HEPES (Fisher Scientific), 1.0 mM sodium pyruvate (GIBCO), and 0.05 mM 2-mercaptoethanol (Sigma-Aldrich) and supplemented with 10% FBS (GIBCO) and 1% antibiotic-antimycotic (GIBCO). .. Cells were maintained at a density of 0.3–1 × 106 cells/ml, and subculturing was performed by centrifuging cells at 100 g for 5 min and resuspending in the appropriate volume of fresh medium.

Slice Preparation:

Article Title: Immunofluorescence staining of live lymph node tissue slices
Article Snippet: Paragraph title: 2.2. Slice preparation ... Complete media consisted of RPMI (Lonza RPMI 1640 without L-glutamine, #12-167F) supplemented with 10% FBS, 1% L-glutamine, and 1% Pen/Strep, 50 μM beta-mercaptoethanol, 1 mM pyruvate, 1% non-essential amino acids, and 20 mM HEPES (Fisher Scientific).

Immunoprecipitation:

Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
Article Snippet: Bacterial cell pellets were suspended in 500 μL of lysis buffer composed of 75 mM NaCl, 3% sodium dodecyl sulfate (SDS) (Fisher Scientific), 1 mM sodium Fluoride (VWR International, LLC), 1 mM beta-glycerophosphate (Sigma Aldrich), 1 mM sodium orthovanadate, 10 mM sodium pyrophosphate (VWR International, LLC), 1 mM phenylmethylsulfonyl fluoride (Fisher Scientific), 50 mM HEPES (Fisher Scientific) pH 8.5, and 1X cOmplete EDTA-free protease inhibitor cocktail, plus 500 μL of 8M Urea (Fisher Scientific), 50 mM HEPES pH 8.5, and subjected to sonication using a Q500 QSonica sonicator (Qsonica) equipped with 1.6 mm microtip at amplitude 20%. .. For immunoprecipitated antigens, the lysis step was omitted, and only 210 μL of 8M Urea, 50 mM HEPES pH 8.5 was added.

Article Title: S-nitrosylation of endogenous protein tyrosine phosphatases in endothelial insulin signaling
Article Snippet: Paragraph title: 2.4. Detection of protein S-nitrosylation in immunoprecipitated PTPs using the PAC-switch method ... Cells were harvested in lysis buffer containing 25 mM HEPES (pH 7.4, BP310-1 from Thermal Fisher Scientific), 150 mM NaCl (BP358-10 from Thermal Fisher Scientific), 1 mM EDTA (disodium salt dihydrate, adjusted to pH 8, S311-500 from Thermal Fisher Scientific), 1% NP-40 (19628 from USB) and protease inhibitors (04-693-132-001 from Roche).

Lysis:

Article Title: Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune Evasion
Article Snippet: .. Bacterial cell pellets were suspended in 500 μL of lysis buffer composed of 75 mM NaCl, 3% sodium dodecyl sulfate (SDS) (Fisher Scientific), 1 mM sodium Fluoride (VWR International, LLC), 1 mM beta-glycerophosphate (Sigma Aldrich), 1 mM sodium orthovanadate, 10 mM sodium pyrophosphate (VWR International, LLC), 1 mM phenylmethylsulfonyl fluoride (Fisher Scientific), 50 mM HEPES (Fisher Scientific) pH 8.5, and 1X cOmplete EDTA-free protease inhibitor cocktail, plus 500 μL of 8M Urea (Fisher Scientific), 50 mM HEPES pH 8.5, and subjected to sonication using a Q500 QSonica sonicator (Qsonica) equipped with 1.6 mm microtip at amplitude 20%. .. Samples were subjected to 10 s of sonication followed by 10 s of rest, with a total sonication time of 30 s. For mouse spleens, 500 μL of lysis buffer and 500 μL of 8M Urea, 50 mM HEPES pH 8.5 was added to homogenized samples in PBS.

Article Title: S-nitrosylation of endogenous protein tyrosine phosphatases in endothelial insulin signaling
Article Snippet: .. Cells were harvested in lysis buffer containing 25 mM HEPES (pH 7.4, BP310-1 from Thermal Fisher Scientific), 150 mM NaCl (BP358-10 from Thermal Fisher Scientific), 1 mM EDTA (disodium salt dihydrate, adjusted to pH 8, S311-500 from Thermal Fisher Scientific), 1% NP-40 (19628 from USB) and protease inhibitors (04-693-132-001 from Roche). .. An aliquot of total lysates (800 μg) was reacted with 50 mM IAM (M216-30G from Amresco) in lysis buffer containing 2.5% SDS (L5750 from Sigma) at room temperature (RT) for 30 min. Proteins were precipitated by cold acetone (100%, 179,124-4L from Sigma) and collected by centrifugation at 2000xg for 5 min, subsequently washed with cold acetone (70%) 3 times.

Fluorescence In Situ Hybridization:

Article Title: Tracking Endocytosis and Intracellular Trafficking of Epitope-tagged Syntaxin 3 by Antibody Feeding in Live, Polarized MDCK Cells
Article Snippet: Sterile 1× Dulbecco’s phosphate buffered saline (DPBS) without calcium or magnesium (Mediatech, catalog number: 21-031-CV) Sterile 0.25% trypsin/EDTA (Mediatech, catalog number: 22-053-CI) 4% buffered formalin solution (Sigma-Aldrich, catalog number: HT5012) Prolong Gold anti-fade reagent plus DAPI (Thermo Fisher Scientific, Invitrogen™, catalog number: ) Sterile Minimum Essential Medium without glutamine (Mediatech, catalog number: 15-010-CV) Sterile L-glutamine 100× (Mediatech, catalog number: 25-005-CI) Penicillin/Streptomycin 100× (Mediatech, catalog number: 30-002-CI) Fetal bovine serum (FBS) (Omega Scientific, catalog number: FB-11) Doxycycline monohydrate (tetracycline analog) (Sigma-Aldrich, catalog number: D1822) HEPES (Fisher Scientific, catalog number: BP310) Bovine serum albumin (BSA) (Sigma-Aldrich, catalog number: A2153) Anti-c-Myc monoclonal antibody, clone 9E10 Note: Hybridoma cells from The Developmental Studies Hybridoma Bank ( ). .. L-glycine (Fisher Scientific, catalog number: BP381-5) Normal Donkey serum (LAMPIRE Biological Labs, catalog number: 7332100) Triton X-100 (Fisher Scientific, catalog number: BP151-500) Mannose-6-Phosphate Receptor antibody (kind gift from William Brown, Cornell University) Fish skin gelatin (Sigma-Aldrich, catalog number: G7765) Donkey anti-mouse DyLight 488 (Jackson ImmunoResearch Laboratories, catalog number: 715-485-150) Note: This product has been discontinued.

Hood:

Article Title: Immunofluorescence staining of live lymph node tissue slices
Article Snippet: Complete media consisted of RPMI (Lonza RPMI 1640 without L-glutamine, #12-167F) supplemented with 10% FBS, 1% L-glutamine, and 1% Pen/Strep, 50 μM beta-mercaptoethanol, 1 mM pyruvate, 1% non-essential amino acids, and 20 mM HEPES (Fisher Scientific). .. For sterile slicing, the procedure differed in the following ways: The agarose was sterilized by autoclaving prior to embedding tissue, 1% Pen-Strep was added to PBS buffer during slicing, the vibratome was placed in a biosafety cabinet to prevent contamination, and the vibratome frequency was reduced to 10 Hz.

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  • 94
    Fisher Scientific hepes buffer
    Titration curve of the competitive displacement assay to determine the extent of labelling on the peptide substrate. (a) Displacement of biotinylated acceptor beads from the StrepTactin ® donor beads by the peptide substrate (b) Bridging of the the StrepTactin ® donor beads and the nickel-chelated donor beads by the peptide substrate, increasing the concentration of substrate results in an increase of signal (c) Cross-titration curve of the peptide substrate and NS2B/NS3 protease which a hook point was reached at 300 nM of peptide substrate. (d) Optimization of <t>HEPES</t> concentration (e) Optimization of <t>NaCl</t> concentration (f) Optimization of BSA concentration (g) Optimization of pH of assay solutions (h) Optimization of incubation time. All data are presented as mean alphasignal ± SEM of triplicates of a single independent experiment. Buffer-Yellow; Peptide-Purple; Peptide and enzyme-Red.
    Hepes Buffer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes buffer/product/Fisher Scientific
    Average 94 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    hepes buffer - by Bioz Stars, 2020-04
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    85
    Fisher Scientific hepes stock solution
    (a) RR and (b) zeta-potential of the 85-nm carboxylate NPs and the 102-nm unfunctionalized NPs in CFs with increasing ionic strength obtained by adding <t>NaCl</t> to 10 mM <t>HEPES</t> (pH 7.3).
    Hepes Stock Solution, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes stock solution/product/Fisher Scientific
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hepes stock solution - by Bioz Stars, 2020-04
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    99
    Fisher Scientific hepes
    binding of ca 2+ ions is determined by membrane charge. titration curves of bound ca 2+ versus added ca 2+ for ca 2+ binding to lipid vesicles composed of (black) 100% dopc, (blue) 55:45 mol% dopc/dops, or (red) 35:30:30:5 mol% dopc/dope/dops/pi(4,5)p 2 . all titrations were performed in 37.5 mm <t>nacl</t> and 7 mm <t>hepes</t> at ph 7, to match saline concentrations used in guv experiments. error bars in the y axis are from the propagation of error in the ise measurement, while x axis error is from the propagation of error from the lipid (determined by phosphate assay) and cacl 2 .
    Hepes, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 99/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes/product/Fisher Scientific
    Average 99 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    hepes - by Bioz Stars, 2020-04
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    Image Search Results


    Titration curve of the competitive displacement assay to determine the extent of labelling on the peptide substrate. (a) Displacement of biotinylated acceptor beads from the StrepTactin ® donor beads by the peptide substrate (b) Bridging of the the StrepTactin ® donor beads and the nickel-chelated donor beads by the peptide substrate, increasing the concentration of substrate results in an increase of signal (c) Cross-titration curve of the peptide substrate and NS2B/NS3 protease which a hook point was reached at 300 nM of peptide substrate. (d) Optimization of HEPES concentration (e) Optimization of NaCl concentration (f) Optimization of BSA concentration (g) Optimization of pH of assay solutions (h) Optimization of incubation time. All data are presented as mean alphasignal ± SEM of triplicates of a single independent experiment. Buffer-Yellow; Peptide-Purple; Peptide and enzyme-Red.

    Journal: Heliyon

    Article Title: Development of a NS2B/NS3 protease inhibition assay using AlphaScreen® beads for screening of anti-dengue activities

    doi: 10.1016/j.heliyon.2018.e01023

    Figure Lengend Snippet: Titration curve of the competitive displacement assay to determine the extent of labelling on the peptide substrate. (a) Displacement of biotinylated acceptor beads from the StrepTactin ® donor beads by the peptide substrate (b) Bridging of the the StrepTactin ® donor beads and the nickel-chelated donor beads by the peptide substrate, increasing the concentration of substrate results in an increase of signal (c) Cross-titration curve of the peptide substrate and NS2B/NS3 protease which a hook point was reached at 300 nM of peptide substrate. (d) Optimization of HEPES concentration (e) Optimization of NaCl concentration (f) Optimization of BSA concentration (g) Optimization of pH of assay solutions (h) Optimization of incubation time. All data are presented as mean alphasignal ± SEM of triplicates of a single independent experiment. Buffer-Yellow; Peptide-Purple; Peptide and enzyme-Red.

    Article Snippet: HEPES buffer, NaOH and NaCl solutions were purchased from Fisher Scientific (Geel, Belgium).

    Techniques: Titration, Concentration Assay, Incubation

    Titration curve of the competitive displacement assay to determine the extent of labelling on the peptide substrate. (a) Displacement of biotinylated acceptor beads from the StrepTactin ® donor beads by the peptide substrate (b) Bridging of the the StrepTactin ® donor beads and the nickel-chelated donor beads by the peptide substrate, increasing the concentration of substrate results in an increase of signal (c) Cross-titration curve of the peptide substrate and NS2B/NS3 protease which a hook point was reached at 300 nM of peptide substrate. (d) Optimization of HEPES concentration (e) Optimization of NaCl concentration (f) Optimization of BSA concentration (g) Optimization of pH of assay solutions (h) Optimization of incubation time. All data are presented as mean alphasignal ± SEM of triplicates of a single independent experiment. Buffer-Yellow; Peptide-Purple; Peptide and enzyme-Red.

    Journal: Heliyon

    Article Title: Development of a NS2B/NS3 protease inhibition assay using AlphaScreen® beads for screening of anti-dengue activities

    doi: 10.1016/j.heliyon.2018.e01023

    Figure Lengend Snippet: Titration curve of the competitive displacement assay to determine the extent of labelling on the peptide substrate. (a) Displacement of biotinylated acceptor beads from the StrepTactin ® donor beads by the peptide substrate (b) Bridging of the the StrepTactin ® donor beads and the nickel-chelated donor beads by the peptide substrate, increasing the concentration of substrate results in an increase of signal (c) Cross-titration curve of the peptide substrate and NS2B/NS3 protease which a hook point was reached at 300 nM of peptide substrate. (d) Optimization of HEPES concentration (e) Optimization of NaCl concentration (f) Optimization of BSA concentration (g) Optimization of pH of assay solutions (h) Optimization of incubation time. All data are presented as mean alphasignal ± SEM of triplicates of a single independent experiment. Buffer-Yellow; Peptide-Purple; Peptide and enzyme-Red.

    Article Snippet: HEPES buffer, NaOH and NaCl solutions were purchased from Fisher Scientific (Geel, Belgium).

    Techniques: Titration, Concentration Assay, Incubation

    (a) RR and (b) zeta-potential of the 85-nm carboxylate NPs and the 102-nm unfunctionalized NPs in CFs with increasing ionic strength obtained by adding NaCl to 10 mM HEPES (pH 7.3).

    Journal: Journal of chromatography. A

    Article Title: Impact of Carrier Fluid Composition on Recovery of Nanoparticles and Proteins in Flow Field Flow Fractionation

    doi: 10.1016/j.chroma.2012.09.050

    Figure Lengend Snippet: (a) RR and (b) zeta-potential of the 85-nm carboxylate NPs and the 102-nm unfunctionalized NPs in CFs with increasing ionic strength obtained by adding NaCl to 10 mM HEPES (pH 7.3).

    Article Snippet: All chemicals used in this study, such as hydrogen chloride (HCl), sodium hydroxide (NaOH), sodium dihydrogen phosphate (NaH2 PO4 ), disodium hydrogen phosphate (Na2 HPO4 ), the chloride salts of sodium, potassium, cesium, and magnesium (NaCl, KCl, CsCl, MgCl2 ), and the 1 M HEPES stock solution (pH 7.3), were acquired from Fisher Scientific (Pittsburgh, PA, USA).

    Techniques:

    binding of ca 2+ ions is determined by membrane charge. titration curves of bound ca 2+ versus added ca 2+ for ca 2+ binding to lipid vesicles composed of (black) 100% dopc, (blue) 55:45 mol% dopc/dops, or (red) 35:30:30:5 mol% dopc/dope/dops/pi(4,5)p 2 . all titrations were performed in 37.5 mm nacl and 7 mm hepes at ph 7, to match saline concentrations used in guv experiments. error bars in the y axis are from the propagation of error in the ise measurement, while x axis error is from the propagation of error from the lipid (determined by phosphate assay) and cacl 2 .

    Journal: Physical chemistry chemical physics : PCCP

    Article Title: Cations induce shape remodeling of negatively charged phospholipid membranes

    doi: 10.1039/c7cp00718c

    Figure Lengend Snippet: binding of ca 2+ ions is determined by membrane charge. titration curves of bound ca 2+ versus added ca 2+ for ca 2+ binding to lipid vesicles composed of (black) 100% dopc, (blue) 55:45 mol% dopc/dops, or (red) 35:30:30:5 mol% dopc/dope/dops/pi(4,5)p 2 . all titrations were performed in 37.5 mm nacl and 7 mm hepes at ph 7, to match saline concentrations used in guv experiments. error bars in the y axis are from the propagation of error in the ise measurement, while x axis error is from the propagation of error from the lipid (determined by phosphate assay) and cacl 2 .

    Article Snippet: CaCl2 , MgCl2 , NaCl, Casein, Tris, HEPES, DTT and EDTA were obtained from Fisher Scientific (Rochester, NY).

    Techniques: Binding Assay, Titration