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Biological Industries Inc hepes
Hepes, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hepes - by Bioz Stars, 2020-03
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Acetylene Reduction Assay:

Article Title: Breaking immune tolerance by targeting Foxp3+ regulatory T cells mitigates Alzheimer's disease pathology
Article Snippet: The cell suspension was washed in culture medium for epithelial cells (DMEM/HAM's F12 (Invitrogen Corp) supplemented with 10% FCS (Sigma-Aldrich), 1 mM l-glutamine, 1 mM sodium pyruvate, 100 U ml−1 penicillin, 100 mg ml−1 streptomycin, 5 μg ml−1 insulin, 20 μM Ara-C, 5 ng ml−1 sodium selenite and 10 ng ml−1 EGF), and cultured (2.5 × 105 cells per well) at 37 °C, 5% CO2 in 24-well plates (Nunc). .. Cells were cultured (1 × 106 cells per well) in complete RPMI 1640 medium w/o phenol red, supplemented with 10% FCS, 100 U ml−1 of penicillin, 100 mg ml−1 of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate and 50 mM β-mercaptoethanol, all from biological industries (Beit Haemek, Israel).

Centrifugation:

Article Title: Leptin promotes cell survival and activates Jurkat T lymphocytes by stimulation of mitogen-activated protein kinase
Article Snippet: Jurkat T cells were cultured in the appropriate medium for cell culture, RPMI-1640, supplemented with 25 mM HEPES, 100 μU/ml l -glutamine, 100 μU/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml amphotericin B and 10% heat-inactivated fetal bovine serum (all from Biological Industries, Kibbutz Beit Haemek, Israel). .. After centrifugation, soluble cell lysates were used for the study.

Article Title: Protein S Negatively Regulates Neural Stem Cell Self-Renewal through Bmi-1 Signaling
Article Snippet: The brains isolated and placed in ice-cold HBSS (Biological Industries) containing 10 mM Hepes (Biological Industries) until dissected midsagitally; the dentate gyri isolated and kept in HBSS/10 mM Hepes on ice. .. The pellet was resuspended in 10 ml Solution 2 [308 mg/ml Sucrose (Sigma) in 0.5 × HBSS], and centrifuged at 2,000 rpm for 10 min, then resuspended in 2 ml Solution 3, and carefully loaded onto 12 ml of Solution 3, and pelleted by centrifugation at 1,500 rpm for 7 min. Cellular debris was removed by centrifugation; the cells resuspended in 15 ml medium in neurosphere growth medium [DMEM:F12, 100 U/ml penicillin; 0.1 mg/ml streptomycin; 2 mM L-Glutamine, 1 × B27 w/o vitamin A (Gibco), 10 mM Hepes].

Cytometry:

Article Title: Breaking immune tolerance by targeting Foxp3+ regulatory T cells mitigates Alzheimer's disease pathology
Article Snippet: Cells were cultured (1 × 106 cells per well) in complete RPMI 1640 medium w/o phenol red, supplemented with 10% FCS, 100 U ml−1 of penicillin, 100 mg ml−1 of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate and 50 mM β-mercaptoethanol, all from biological industries (Beit Haemek, Israel). .. After 4 days in culture, FoxP3 expression was verified using flow cytometry of GFP-expressing cells, and the cells were added to the CP cultures in a 1:1 epithelial/Treg ratio.

Article Title: Identification and Characterization of Two Human Monocyte-Derived Dendritic Cell Subpopulations with Different Functions in Dying Cell Clearance and Different Patterns of Cell Death
Article Snippet: Paragraph title: Cytometry ... 6) The staining buffer consisted of PBS without calcium, supplemented with HEPES and 1% fetal calf serum (all from Biological Industries).

Construct:

Article Title: The pain receptor TRPV1 displays agonist-dependent activation stoichiometry
Article Snippet: Cell culture and transfection Human embryonic kidney 293 cells (HEK293T) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich, MO, USA) supplemented with 10% fetal bovine serum (FBS), 1% Penicillin-Streptomycin, 2 mM l-Alanyl l-Glutamine, and 25 mM HEPES (pH 7.3; Biological industries, Israel) (herein: Full DMEM) at 37 °C and 5% CO2 . .. Generally, 300 ng of rTRPV1 in pCDNA3.1+ was used for transfection, while 700 ng of any of the concatemeric constructs expressed in pCDNA3.1+ were used.

Modification:

Article Title: The pain receptor TRPV1 displays agonist-dependent activation stoichiometry
Article Snippet: .. Cell culture and transfection Human embryonic kidney 293 cells (HEK293T) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich, MO, USA) supplemented with 10% fetal bovine serum (FBS), 1% Penicillin-Streptomycin, 2 mM l-Alanyl l-Glutamine, and 25 mM HEPES (pH 7.3; Biological industries, Israel) (herein: Full DMEM) at 37 °C and 5% CO2 . .. Transfections were performed in 12-well plates containing 3 × 105 cells using Mirus LT1 transfection reagent (Mirus Bio, WI, USA) with Opti-MEM I Reduced Serum Medium (Invitrogen, MA, USA).

Incubation:

Article Title: Drosophila TRF2 is a preferential core promoter regulator
Article Snippet: Hepes (50 mM; Biological Industries) was used for washing unreacted substrates between each of the different surface chemistry steps. .. DNA that interacted with loaded proteins was captured beneath the button valve following a 1-h incubation period.

Article Title: Inhibition of NKCC1 Modulates Alveolar Fluid Clearance and Inflammation in Ischemia-Reperfusion Lung Injury via TRAF6-Mediated Pathways
Article Snippet: The cells were cultured in DMEM/F-12 media (Biological Industries, Beth Haemek, Israel), which contained 2% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA) and other relevant supplements: 5 μg/ml of insulin,10 μg/ml of transferrin, 2 mM of L-glutamine, 10 mM of HEPES (Biological Industries, Beth Haemek, Israel), 30 nM of sodium selenite, 10 nM of hydrocortisone, and 10 nM of β-estradiol (Sigma-Aldrich, St. Louis, MO, USA) at 37°C in a humidified atmosphere containing 5% CO2 . .. The cells were passaged at approximately 80% confluence by incubation with 0.25% trypsin-EDTA.

Expressing:

Article Title: Functional Relevance of the Switch of VEGF Receptors/Co-Receptors during Peritoneal Dialysis-Induced Mesothelial to Mesenchymal Transition
Article Snippet: All cells were cultured in Earle’s M199 medium, supplemented with 20% fetal-calf serum (FCS), 2% Hepes 1 M, 50 U/mL penicillin, 50 µg/mL streptomycin and 2% Biogro-2 (Biological Industries, Beit Haemek, Israel). .. The purity of effluent and omentum-derived MC cultures was determined by the expression of standard mesothelial markers: intercellular adhesion molecule-1, cytokeratins, and calretinine.

Article Title: Breaking immune tolerance by targeting Foxp3+ regulatory T cells mitigates Alzheimer's disease pathology
Article Snippet: Cells were cultured (1 × 106 cells per well) in complete RPMI 1640 medium w/o phenol red, supplemented with 10% FCS, 100 U ml−1 of penicillin, 100 mg ml−1 of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate and 50 mM β-mercaptoethanol, all from biological industries (Beit Haemek, Israel). .. After 4 days in culture, FoxP3 expression was verified using flow cytometry of GFP-expressing cells, and the cells were added to the CP cultures in a 1:1 epithelial/Treg ratio.

Article Title: Expansion and Antitumor Cytotoxicity of T-Cells Are Augmented by Substrate-Bound CCL21 and Intercellular Adhesion Molecule 1
Article Snippet: Paragraph title: Measuring Granzyme B, FasL, and PD-1 Expression Level ... B16 cells-expressing ovalbumin (courtesy of Lea Eisenach, Weizmann institute of Science) were suspended in DMEM medium w/o phenol red, supplemented with 10% fetal bovine serum, 100 U/ml of penicillin, 100 mg/ml of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate, and 50 mM β-mercaptoethanol (Biological Industries).

Article Title: Expansion and Antitumor Cytotoxicity of T-Cells Are Augmented by Substrate-Bound CCL21 and Intercellular Adhesion Molecule 1
Article Snippet: .. In Vitro Cytotoxic Murine T-Cell Killing Assay B16 cells expressing ovalbumin coupled with GFP (courtesy of Guy Shakhar, Weizmann institute of Science) were suspended in RPMI 1640 medium w/o phenol red, supplemented with 10% serum, 100 U/ml of penicillin, 100 mg/ml of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate, and 50 mM β-mercaptoethanol (Biological Industries). ..

Ex Vivo:

Article Title: Breaking immune tolerance by targeting Foxp3+ regulatory T cells mitigates Alzheimer's disease pathology
Article Snippet: For ex vivo differentiation of Tregs, splenocytes were collected from 5 to 8-week-old FoxP3-GFP mice, and following lysis of erythrocytes naive T cells were magnetically separated using a CD4+ CD62L+ T-Cell Isolation Kit (Miltenyi Biotec, Germany). .. Cells were cultured (1 × 106 cells per well) in complete RPMI 1640 medium w/o phenol red, supplemented with 10% FCS, 100 U ml−1 of penicillin, 100 mg ml−1 of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate and 50 mM β-mercaptoethanol, all from biological industries (Beit Haemek, Israel).

Derivative Assay:

Article Title: SOX9 indirectly regulates CEACAM1 expression and immune resistance in melanoma cells
Article Snippet: 009mel and 014mel are primary cultures derived from surgically removed metastatic melanoma specimens of patients 009 and 14, respectively. .. Melanoma cultures were grown in RPMI-1640 medium (Biological Industries, Beit Ha-Emek, Israel) supplemented with 10% FBS, 100 μg/ml Pen/Strep, 2mM L-glutamine, 25mM Hepes and 1mM sodium-pyruvate (Biological Industries, Beit Ha-Emek, Israel).

Flow Cytometry:

Article Title: Drosophila TRF2 is a preferential core promoter regulator
Article Snippet: Following passivation, the “button” valve was released, and a flow of 0.2 µg/µL anti-Flag biotinylated antibodies (Cell Signaling) was applied. .. Hepes (50 mM; Biological Industries) was used for washing unreacted substrates between each of the different surface chemistry steps.

Article Title: Breaking immune tolerance by targeting Foxp3+ regulatory T cells mitigates Alzheimer's disease pathology
Article Snippet: Cells were cultured (1 × 106 cells per well) in complete RPMI 1640 medium w/o phenol red, supplemented with 10% FCS, 100 U ml−1 of penicillin, 100 mg ml−1 of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate and 50 mM β-mercaptoethanol, all from biological industries (Beit Haemek, Israel). .. After 4 days in culture, FoxP3 expression was verified using flow cytometry of GFP-expressing cells, and the cells were added to the CP cultures in a 1:1 epithelial/Treg ratio.

Transfection:

Article Title: The pain receptor TRPV1 displays agonist-dependent activation stoichiometry
Article Snippet: .. Cell culture and transfection Human embryonic kidney 293 cells (HEK293T) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich, MO, USA) supplemented with 10% fetal bovine serum (FBS), 1% Penicillin-Streptomycin, 2 mM l-Alanyl l-Glutamine, and 25 mM HEPES (pH 7.3; Biological industries, Israel) (herein: Full DMEM) at 37 °C and 5% CO2 . .. Transfections were performed in 12-well plates containing 3 × 105 cells using Mirus LT1 transfection reagent (Mirus Bio, WI, USA) with Opti-MEM I Reduced Serum Medium (Invitrogen, MA, USA).

Cell Culture:

Article Title: Functional Relevance of the Switch of VEGF Receptors/Co-Receptors during Peritoneal Dialysis-Induced Mesothelial to Mesenchymal Transition
Article Snippet: .. All cells were cultured in Earle’s M199 medium, supplemented with 20% fetal-calf serum (FCS), 2% Hepes 1 M, 50 U/mL penicillin, 50 µg/mL streptomycin and 2% Biogro-2 (Biological Industries, Beit Haemek, Israel). .. The purity of effluent and omentum-derived MC cultures was determined by the expression of standard mesothelial markers: intercellular adhesion molecule-1, cytokeratins, and calretinine.

Article Title: The pain receptor TRPV1 displays agonist-dependent activation stoichiometry
Article Snippet: .. Cell culture and transfection Human embryonic kidney 293 cells (HEK293T) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich, MO, USA) supplemented with 10% fetal bovine serum (FBS), 1% Penicillin-Streptomycin, 2 mM l-Alanyl l-Glutamine, and 25 mM HEPES (pH 7.3; Biological industries, Israel) (herein: Full DMEM) at 37 °C and 5% CO2 . .. Transfections were performed in 12-well plates containing 3 × 105 cells using Mirus LT1 transfection reagent (Mirus Bio, WI, USA) with Opti-MEM I Reduced Serum Medium (Invitrogen, MA, USA).

Article Title: SOX9 indirectly regulates CEACAM1 expression and immune resistance in melanoma cells
Article Snippet: Melanoma cultures were grown in RPMI-1640 medium (Biological Industries, Beit Ha-Emek, Israel) supplemented with 10% FBS, 100 μg/ml Pen/Strep, 2mM L-glutamine, 25mM Hepes and 1mM sodium-pyruvate (Biological Industries, Beit Ha-Emek, Israel). .. The A375 human metastatic melanoma cell line (ATCC, Manassas, VA, USA) was cultured in DMEM medium (Biological Industries, Beit Ha-Emek, Israel) supplemented with 10% FBS, 100 μg/ml Pen/Strep, 2mM L-glutamine, 1mM sodium-pyruvate and non-essential amino-acids (Biological Industries, Beit Ha-Emek, Israel).

Article Title: Leptin promotes cell survival and activates Jurkat T lymphocytes by stimulation of mitogen-activated protein kinase
Article Snippet: .. Jurkat T cells were cultured in the appropriate medium for cell culture, RPMI-1640, supplemented with 25 mM HEPES, 100 μU/ml l -glutamine, 100 μU/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml amphotericin B and 10% heat-inactivated fetal bovine serum (all from Biological Industries, Kibbutz Beit Haemek, Israel). ..

Article Title: Breaking immune tolerance by targeting Foxp3+ regulatory T cells mitigates Alzheimer's disease pathology
Article Snippet: .. Cells were cultured (1 × 106 cells per well) in complete RPMI 1640 medium w/o phenol red, supplemented with 10% FCS, 100 U ml−1 of penicillin, 100 mg ml−1 of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate and 50 mM β-mercaptoethanol, all from biological industries (Beit Haemek, Israel). .. To drive differentiation of naive CD4+ T cells to a regulatory phenotype (Treg cells), growth medium was supplemented also with 5 ng ml−1 IL-2 and 10 ng ml−1 of recombinant human TGFb (both purchased from R & D systems).

Article Title: Potential of Direct Agglutination Test Based on Promastigote and Amastigote Antigens for Serodiagnosis of Post-Kala-Azar Dermal Leishmaniasis
Article Snippet: Parasite cultures were set up from bone marrow samples from KA patients or dermal lesions of PKDL patients and propagated as promastigotes in M-199 (Sigma-Aldrich, Steinheim, Germany) supplemented with 25 mM HEPES (pH 7.5) and 10% fetal calf serum (Biological Industries, Israel) as described earlier ( ). .. Axenic amastigotes from an indigenous isolate of L. donovani were cultured by gradual adaptation of promastigotes to grow at pH 5.5 and 37°C in 6% CO2 atmosphere as described earlier ( , ).

Article Title: Inhibition of NKCC1 Modulates Alveolar Fluid Clearance and Inflammation in Ischemia-Reperfusion Lung Injury via TRAF6-Mediated Pathways
Article Snippet: .. The cells were cultured in DMEM/F-12 media (Biological Industries, Beth Haemek, Israel), which contained 2% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA) and other relevant supplements: 5 μg/ml of insulin,10 μg/ml of transferrin, 2 mM of L-glutamine, 10 mM of HEPES (Biological Industries, Beth Haemek, Israel), 30 nM of sodium selenite, 10 nM of hydrocortisone, and 10 nM of β-estradiol (Sigma-Aldrich, St. Louis, MO, USA) at 37°C in a humidified atmosphere containing 5% CO2 . .. The cells were passaged at approximately 80% confluence by incubation with 0.25% trypsin-EDTA.

Article Title: Expansion and Antitumor Cytotoxicity of T-Cells Are Augmented by Substrate-Bound CCL21 and Intercellular Adhesion Molecule 1
Article Snippet: B16 cells-expressing ovalbumin (courtesy of Lea Eisenach, Weizmann institute of Science) were suspended in DMEM medium w/o phenol red, supplemented with 10% fetal bovine serum, 100 U/ml of penicillin, 100 mg/ml of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate, and 50 mM β-mercaptoethanol (Biological Industries). .. Cells were seeded 50–100 × 103 B16 cells per well in a 24-well plate; 150–300 × 103 OT-I T-cells pre-activated and cultured for 7 days, were added on top of the B16 cells.

Article Title: Expansion and Antitumor Cytotoxicity of T-Cells Are Augmented by Substrate-Bound CCL21 and Intercellular Adhesion Molecule 1
Article Snippet: In Vitro Cytotoxic Murine T-Cell Killing Assay B16 cells expressing ovalbumin coupled with GFP (courtesy of Guy Shakhar, Weizmann institute of Science) were suspended in RPMI 1640 medium w/o phenol red, supplemented with 10% serum, 100 U/ml of penicillin, 100 mg/ml of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate, and 50 mM β-mercaptoethanol (Biological Industries). .. OT-I T-cells activated and cultured for 3 or 7 days as described above, were then added on top of the B16 cells.

Article Title: Retama monosperma n-hexane extract induces cell cycle arrest and extrinsic pathway-dependent apoptosis in Jurkat cells
Article Snippet: .. Cell culture Jurkat, TK6 and Jeko-1 cells were maintained in RPMI-1640 with L-Glutamine and HEPES (Biological Industries Ltd., Kibbutz Beit Haemek, Israel). .. LN229, T98G, U87MG, SW620, SW480, U2OS, PC-3 and NIH3T3 cells were maintained in DMEM High Glucose (4.5 g/l) with L-glutamine (Invitrogen, Carlsbad, CA).

other:

Article Title: Cancer-selective cytotoxic Ca2+ overload in acute myeloid leukemia cells and attenuation of disease progression in mice by synergistically acting polyphenols curcumin and carnosic acid
Article Snippet: RPMI 1640 medium, Ca2+ /Mg2+ -free phosphate buffered saline (PBS), penicillin, streptomycin, and HEPES were purchased from Biological Industries (Beth Haemek, Israel).

Polymerase Chain Reaction:

Article Title: Potential of Direct Agglutination Test Based on Promastigote and Amastigote Antigens for Serodiagnosis of Post-Kala-Azar Dermal Leishmaniasis
Article Snippet: Parasite cultures were set up from bone marrow samples from KA patients or dermal lesions of PKDL patients and propagated as promastigotes in M-199 (Sigma-Aldrich, Steinheim, Germany) supplemented with 25 mM HEPES (pH 7.5) and 10% fetal calf serum (Biological Industries, Israel) as described earlier ( ). .. The indigenous isolates of parasites causing KA and PKDL were characterized as L. donovani using monoclonal antibodies and a species-specific PCR ( , ).

Injection:

Article Title: Development of Foreign Mammary Epithelial Morphology in the Stroma of Immunodeficient Mice
Article Snippet: The cells were suspended in 20 µl Matrigel (BD Biosciences) diluted 1:1 (v/v) in HF solution containing Hank’s balanced salt solution (HBSS, Biological Industries) supplemented with 0.1% Hepes (Biological Industries) and 2% fetal bovine serum (FBS, Biological Industries). .. Cell suspension in Matrigel was injected into the cleared mammary fat pad using a 50-µl Hamilton syringe (Hamilton Company, Reno, NV) equipped with a 21-gauge needle.

Recombinant:

Article Title: Breaking immune tolerance by targeting Foxp3+ regulatory T cells mitigates Alzheimer's disease pathology
Article Snippet: Cells were cultured (1 × 106 cells per well) in complete RPMI 1640 medium w/o phenol red, supplemented with 10% FCS, 100 U ml−1 of penicillin, 100 mg ml−1 of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate and 50 mM β-mercaptoethanol, all from biological industries (Beit Haemek, Israel). .. To drive differentiation of naive CD4+ T cells to a regulatory phenotype (Treg cells), growth medium was supplemented also with 5 ng ml−1 IL-2 and 10 ng ml−1 of recombinant human TGFb (both purchased from R & D systems).

Isolation:

Article Title: Functional Relevance of the Switch of VEGF Receptors/Co-Receptors during Peritoneal Dialysis-Induced Mesothelial to Mesenchymal Transition
Article Snippet: Paragraph title: Isolation and Culture of Mesothelial Cells and Treatments ... All cells were cultured in Earle’s M199 medium, supplemented with 20% fetal-calf serum (FCS), 2% Hepes 1 M, 50 U/mL penicillin, 50 µg/mL streptomycin and 2% Biogro-2 (Biological Industries, Beit Haemek, Israel).

Article Title: Breaking immune tolerance by targeting Foxp3+ regulatory T cells mitigates Alzheimer's disease pathology
Article Snippet: For ex vivo differentiation of Tregs, splenocytes were collected from 5 to 8-week-old FoxP3-GFP mice, and following lysis of erythrocytes naive T cells were magnetically separated using a CD4+ CD62L+ T-Cell Isolation Kit (Miltenyi Biotec, Germany). .. Cells were cultured (1 × 106 cells per well) in complete RPMI 1640 medium w/o phenol red, supplemented with 10% FCS, 100 U ml−1 of penicillin, 100 mg ml−1 of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate and 50 mM β-mercaptoethanol, all from biological industries (Beit Haemek, Israel).

Article Title: Protein S Negatively Regulates Neural Stem Cell Self-Renewal through Bmi-1 Signaling
Article Snippet: .. The brains isolated and placed in ice-cold HBSS (Biological Industries) containing 10 mM Hepes (Biological Industries) until dissected midsagitally; the dentate gyri isolated and kept in HBSS/10 mM Hepes on ice. .. The tissue was enzymatically dissociated in 5 ml of Solution 1 [5.4 mg/ml D-Glucose (Sigma), 15 mM HEPES (Biological Industries) 680 ng/ml Trypsin (Sigma) and 700 ng/ml hyaluronidase (Sigma) in 1 × HBSS] for 30 min at 37°C, with gentle trituration after 15 min.

Article Title: Potential of Direct Agglutination Test Based on Promastigote and Amastigote Antigens for Serodiagnosis of Post-Kala-Azar Dermal Leishmaniasis
Article Snippet: Parasite cultures were set up from bone marrow samples from KA patients or dermal lesions of PKDL patients and propagated as promastigotes in M-199 (Sigma-Aldrich, Steinheim, Germany) supplemented with 25 mM HEPES (pH 7.5) and 10% fetal calf serum (Biological Industries, Israel) as described earlier ( ). .. Promastigotes of L. donovani strain AG83 (MHOM/IN/AG/83) originally isolated from a KA patient were also grown as described above.

Avidin-Biotin Assay:

Article Title: Drosophila TRF2 is a preferential core promoter regulator
Article Snippet: The antibody bound specifically to the exposed Avidin surface under the “button,” creating an anti-Flag tag array. .. Hepes (50 mM; Biological Industries) was used for washing unreacted substrates between each of the different surface chemistry steps.

Microscopy:

Article Title: Breaking immune tolerance by targeting Foxp3+ regulatory T cells mitigates Alzheimer's disease pathology
Article Snippet: In vitro experiments Following intracardial perfusion with PBS, the CPs were removed from the third, fourth, and lateral ventricles, under a dissecting microscope (Stemi DV4), and placed in tubes containing 0.25% trypsin (Life Technologies #15090-046). .. Cells were cultured (1 × 106 cells per well) in complete RPMI 1640 medium w/o phenol red, supplemented with 10% FCS, 100 U ml−1 of penicillin, 100 mg ml−1 of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate and 50 mM β-mercaptoethanol, all from biological industries (Beit Haemek, Israel).

Article Title: Expansion and Antitumor Cytotoxicity of T-Cells Are Augmented by Substrate-Bound CCL21 and Intercellular Adhesion Molecule 1
Article Snippet: In Vitro Cytotoxic Murine T-Cell Killing Assay B16 cells expressing ovalbumin coupled with GFP (courtesy of Guy Shakhar, Weizmann institute of Science) were suspended in RPMI 1640 medium w/o phenol red, supplemented with 10% serum, 100 U/ml of penicillin, 100 mg/ml of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate, and 50 mM β-mercaptoethanol (Biological Industries). .. The entire well was imaged every 6 h, using a Hermes® microscope (IDEA BioMedical Ltd.) equipped with automated scanning optics, high-precision autofocus, and a closed environmental chamber.

Purification:

Article Title: Identification and Characterization of Two Human Monocyte-Derived Dendritic Cell Subpopulations with Different Functions in Dying Cell Clearance and Different Patterns of Cell Death
Article Snippet: Alternatively, purified antibodies were used, in which case secondary staining was performed using goat anti-mouse antibodies in the presence of 75 μg/mL goat Ig. .. 6) The staining buffer consisted of PBS without calcium, supplemented with HEPES and 1% fetal calf serum (all from Biological Industries).

Staining:

Article Title: Expansion and Antitumor Cytotoxicity of T-Cells Are Augmented by Substrate-Bound CCL21 and Intercellular Adhesion Molecule 1
Article Snippet: B16 cells-expressing ovalbumin (courtesy of Lea Eisenach, Weizmann institute of Science) were suspended in DMEM medium w/o phenol red, supplemented with 10% fetal bovine serum, 100 U/ml of penicillin, 100 mg/ml of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate, and 50 mM β-mercaptoethanol (Biological Industries). .. After 24–48 h, cells were collected, and a single-cell suspension was prepared and stained with live/dead stain ( , Life Technologies, Carlsbad, CA, USA), CD8 (BLG100734, Biolegend), FasL (BLG106606, Biolegend), and PD-1 (BLG135225, Biolegend).

Article Title: Identification and Characterization of Two Human Monocyte-Derived Dendritic Cell Subpopulations with Different Functions in Dying Cell Clearance and Different Patterns of Cell Death
Article Snippet: .. 6) The staining buffer consisted of PBS without calcium, supplemented with HEPES and 1% fetal calf serum (all from Biological Industries). ..

Mouse Assay:

Article Title: Breaking immune tolerance by targeting Foxp3+ regulatory T cells mitigates Alzheimer's disease pathology
Article Snippet: For ex vivo differentiation of Tregs, splenocytes were collected from 5 to 8-week-old FoxP3-GFP mice, and following lysis of erythrocytes naive T cells were magnetically separated using a CD4+ CD62L+ T-Cell Isolation Kit (Miltenyi Biotec, Germany). .. Cells were cultured (1 × 106 cells per well) in complete RPMI 1640 medium w/o phenol red, supplemented with 10% FCS, 100 U ml−1 of penicillin, 100 mg ml−1 of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate and 50 mM β-mercaptoethanol, all from biological industries (Beit Haemek, Israel).

Article Title: Protein S Negatively Regulates Neural Stem Cell Self-Renewal through Bmi-1 Signaling
Article Snippet: NSC isolation from adult dentate gyrus Eight to twelve weeks old mice were deeply anesthetized and sacrificed. .. The brains isolated and placed in ice-cold HBSS (Biological Industries) containing 10 mM Hepes (Biological Industries) until dissected midsagitally; the dentate gyri isolated and kept in HBSS/10 mM Hepes on ice.

Article Title: Development of Foreign Mammary Epithelial Morphology in the Stroma of Immunodeficient Mice
Article Snippet: When hormonal administration was involved, bMECs were transplanted 2 weeks after clearing, when the mice were 5 weeks old. .. The cells were suspended in 20 µl Matrigel (BD Biosciences) diluted 1:1 (v/v) in HF solution containing Hank’s balanced salt solution (HBSS, Biological Industries) supplemented with 0.1% Hepes (Biological Industries) and 2% fetal bovine serum (FBS, Biological Industries).

Binding Assay:

Article Title: Drosophila TRF2 is a preferential core promoter regulator
Article Snippet: To derivatize the slide surface, 1 µg/µL biotinylated-BSA (Thermo) was flown for 30 min through the device to allow binding of the BSA to the epoxy surface. .. Hepes (50 mM; Biological Industries) was used for washing unreacted substrates between each of the different surface chemistry steps.

Article Title: Identification and Characterization of Two Human Monocyte-Derived Dendritic Cell Subpopulations with Different Functions in Dying Cell Clearance and Different Patterns of Cell Death
Article Snippet: Bad samples, identified by abnormally high isotype binding, were excluded. .. 6) The staining buffer consisted of PBS without calcium, supplemented with HEPES and 1% fetal calf serum (all from Biological Industries).

In Vitro:

Article Title: Functional Relevance of the Switch of VEGF Receptors/Co-Receptors during Peritoneal Dialysis-Induced Mesothelial to Mesenchymal Transition
Article Snippet: All cells were cultured in Earle’s M199 medium, supplemented with 20% fetal-calf serum (FCS), 2% Hepes 1 M, 50 U/mL penicillin, 50 µg/mL streptomycin and 2% Biogro-2 (Biological Industries, Beit Haemek, Israel). .. To induce MMT in vitro , omentum-derived MCs were treated for 72 hours with a combination of human-recombinant TGF-β1 (0.5 ng/mL) and IL-1β (2 ng/mL) (T+I) (R & D Systems, Inc, Minneapolis, MN), which has been proven to be a good in vitro model of MMT , , , , .

Article Title: Breaking immune tolerance by targeting Foxp3+ regulatory T cells mitigates Alzheimer's disease pathology
Article Snippet: Paragraph title: In vitro experiments ... Cells were cultured (1 × 106 cells per well) in complete RPMI 1640 medium w/o phenol red, supplemented with 10% FCS, 100 U ml−1 of penicillin, 100 mg ml−1 of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate and 50 mM β-mercaptoethanol, all from biological industries (Beit Haemek, Israel).

Article Title: Expansion and Antitumor Cytotoxicity of T-Cells Are Augmented by Substrate-Bound CCL21 and Intercellular Adhesion Molecule 1
Article Snippet: .. In Vitro Cytotoxic Murine T-Cell Killing Assay B16 cells expressing ovalbumin coupled with GFP (courtesy of Guy Shakhar, Weizmann institute of Science) were suspended in RPMI 1640 medium w/o phenol red, supplemented with 10% serum, 100 U/ml of penicillin, 100 mg/ml of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate, and 50 mM β-mercaptoethanol (Biological Industries). ..

Lysis:

Article Title: Leptin promotes cell survival and activates Jurkat T lymphocytes by stimulation of mitogen-activated protein kinase
Article Snippet: Jurkat T cells were cultured in the appropriate medium for cell culture, RPMI-1640, supplemented with 25 mM HEPES, 100 μU/ml l -glutamine, 100 μU/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml amphotericin B and 10% heat-inactivated fetal bovine serum (all from Biological Industries, Kibbutz Beit Haemek, Israel). .. Cell cultures were centrifuged to remove medium and were solubilized for 30 min at 4°C in lysis buffer containing 20 mM Tris, pH 8, 1% Nonidet P-40, 137 mM NaCl, 1 mM MgCl2 , 1 mM CaCl2 , 1 mM dithiothreitol (DTT), 10% glycerol, 1 mM phenylmethylsulphonylfluoride and 0·4 mM sodium orthovanadate.

Article Title: Breaking immune tolerance by targeting Foxp3+ regulatory T cells mitigates Alzheimer's disease pathology
Article Snippet: For ex vivo differentiation of Tregs, splenocytes were collected from 5 to 8-week-old FoxP3-GFP mice, and following lysis of erythrocytes naive T cells were magnetically separated using a CD4+ CD62L+ T-Cell Isolation Kit (Miltenyi Biotec, Germany). .. Cells were cultured (1 × 106 cells per well) in complete RPMI 1640 medium w/o phenol red, supplemented with 10% FCS, 100 U ml−1 of penicillin, 100 mg ml−1 of streptomycin, 2 mM glutamine, 10 mM HEPES, 1 mM sodium pyruvate and 50 mM β-mercaptoethanol, all from biological industries (Beit Haemek, Israel).

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    Biological Industries Inc hepes
    Hepes, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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