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Beyotime hepes
Hepes, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hepes/product/Beyotime
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
hepes - by Bioz Stars, 2020-03
99/100 stars

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Related Articles

ROS Assay:

Article Title: Antibacterial Effect and Mode of Action of Flavonoids From Licorice Against Methicillin-Resistant Staphylococcus aureus
Article Snippet: .. Reactive Oxygen Species (ROS) Assay S. aureus ATCC29213 cells were washed three times with buffer containing 5 mM HEPES and 20 mM glucose at pH 7.2, adjusted to obtain a bacterial suspension with densities equivalent to a 0.5 McFarland turbidity standard, and then incubated with 10 μM dichloro-dihydro-fluorescein diacetate (DCFH-DA; Beyotime, Shanghai, China) at 37°C for 30 min. After incubation, the S. aureus cells were treated with different concentrations of drugs for 60 min, and the fluorescence intensity of the DCFH-DA dye was monitored using a Tecan Infinite 200 Pro Microplate Reader at an excitation wavelength of 488 nm and an emission wavelength of 525 nm ( ). .. Confocal Microscopy Assay S. aureus ATCC29213 cells were washed three times with PBS and then incubated with glabrol and lysostaphin for 30 min. After incubation, bacterial cells were washed with PBS and then stained with 5 μM PI and 10 μM SYTOTM 9 Green Fluorescent Nucleic Acid Stain (SYTO 9; Thermo Fisher Scientific, Waltham, MA, United States).

Transfection:

Article Title: Correlation between the expression of IL-18 and deep venous thrombosis
Article Snippet: .. For packaging and infection of the viral vectors, the pCDH-GFP lentivirus (12 g) was packaged with the pCL-ECO plasmid (8 g) and was transfected into the overexpression plasmid IL-18-pCDH-GFP using HEPES and the Calcium Phosphate Cell Transfection kit (Beyotime Institute of Biotechnology, Jiangsu, China) according to the manufacturer's protocols Normal cells and cells transfected with null vectors were used as controls. ..

Over Expression:

Article Title: Correlation between the expression of IL-18 and deep venous thrombosis
Article Snippet: .. For packaging and infection of the viral vectors, the pCDH-GFP lentivirus (12 g) was packaged with the pCL-ECO plasmid (8 g) and was transfected into the overexpression plasmid IL-18-pCDH-GFP using HEPES and the Calcium Phosphate Cell Transfection kit (Beyotime Institute of Biotechnology, Jiangsu, China) according to the manufacturer's protocols Normal cells and cells transfected with null vectors were used as controls. ..

Fluorescence:

Article Title: Antibacterial Effect and Mode of Action of Flavonoids From Licorice Against Methicillin-Resistant Staphylococcus aureus
Article Snippet: .. Reactive Oxygen Species (ROS) Assay S. aureus ATCC29213 cells were washed three times with buffer containing 5 mM HEPES and 20 mM glucose at pH 7.2, adjusted to obtain a bacterial suspension with densities equivalent to a 0.5 McFarland turbidity standard, and then incubated with 10 μM dichloro-dihydro-fluorescein diacetate (DCFH-DA; Beyotime, Shanghai, China) at 37°C for 30 min. After incubation, the S. aureus cells were treated with different concentrations of drugs for 60 min, and the fluorescence intensity of the DCFH-DA dye was monitored using a Tecan Infinite 200 Pro Microplate Reader at an excitation wavelength of 488 nm and an emission wavelength of 525 nm ( ). .. Confocal Microscopy Assay S. aureus ATCC29213 cells were washed three times with PBS and then incubated with glabrol and lysostaphin for 30 min. After incubation, bacterial cells were washed with PBS and then stained with 5 μM PI and 10 μM SYTOTM 9 Green Fluorescent Nucleic Acid Stain (SYTO 9; Thermo Fisher Scientific, Waltham, MA, United States).

Patch Clamp:

Article Title: Abnormal alterations in the Ca2+/CaV1.2/calmodulin/caMKII signaling pathway in a tremor rat model and in cultured hippocampal neurons exposed to Mg2+-free solution
Article Snippet: The pipette solution contained 50 mM K-Aspartate, 20 mM KCl, 20 mM HEPES, 1 mM EGTA, 1 mM MgCl2 , 0.2 mM CaCl2 , 13.6 mM NaCl and 3 mM K2ATP3 (pH 7.4; KOH; Beyotime Institute of Biotechnology). .. Patch-clamp electrodes were obtained with capillary tubes pulled with a P-97 puller (Gibco-BRL).

Cell Culture:

Article Title: HIV-1 Tat Regulates Occludin and Aβ Transfer Receptor Expression in Brain Endothelial Cells via Rho/ROCK Signaling Pathway
Article Snippet: Endothelial basal medium (EBM)-2 (Lonza, Walkersville, MD, USA) was supplemented with 5% fetal bovine serum (Lonza, Switzerland), 1 ng/mL basic fibroblast growth factor (Sigma-Aldrich, USA), 5 µ g/mL ascorbic acid (Sigma-Aldrich), 1/100 chemically defined lipid concentrate (Gibco, NY, USA), 10 mmol/L HEPES (Beyotime, Jiangsu, China), 1.4 µ mol/L hydrocortisone (Sigma-Aldrich, USA), and 1% penicillin-streptomycin (Beyotime), as recommended by the manufacturer (Lonza, Walkersville, MD, USA). .. This solution is called complete EBM-2 medium. hCMEC/D3 cells were seeded into flasks coated with 1 mg/mL collagen type I (R & D Systems) and cultured in complete EBM-2 medium, maintained at 37°C in a humidified atmosphere of 5% CO2 .

Article Title: High Mobility Group Protein 1 Reverses Immune System Paralysis in Late-Phase Sepsis
Article Snippet: RPMI 1640 medium, fetal calf serum (FCS), glutamine, penicillin, streptomycin, and HEPES were purchased from Beyotime Biotech Co. Ltd., Shanghai, China. .. Polymyxin B was added to the cell culture medium at 10 μg/ml to neutralize the activity of endotoxin.

Article Title: Abnormal alterations in the Ca2+/CaV1.2/calmodulin/caMKII signaling pathway in a tremor rat model and in cultured hippocampal neurons exposed to Mg2+-free solution
Article Snippet: The pipette solution contained 50 mM K-Aspartate, 20 mM KCl, 20 mM HEPES, 1 mM EGTA, 1 mM MgCl2 , 0.2 mM CaCl2 , 13.6 mM NaCl and 3 mM K2ATP3 (pH 7.4; KOH; Beyotime Institute of Biotechnology). .. To record action potential, cultures, including the normal cultured neurons and the neurons treated with Mg2+ -free solution, were transferred to the stage of an inverted microscope (TMS-1015, Olympus, Tokyo, Japan).

Article Title: Specific-detection of clinical samples, systematic functional investigations, and transcriptome analysis reveals that splice variant MUC4/Y contributes to the malignant progression of pancreatic cancer by triggering malignancy-related positive feedback loops signaling
Article Snippet: .. Immunofluorescence Cells cultured to 70% confluence were washed with 0.1 mol/L HEPES containing Hanks’ buffer and fixed with Immunol Staining Fix Solution (#P0098; Beyotime) at room temperature for 15 min. After three 10-min washes in Immunol Staining Wash Buffer (#P0106; Beyotime), the fixed cells were blocked in Immunol Staining Blocking Buffer (#P0102; Beyotime) for 60 min at room temperature for nonspecific blocking, followed by incubation at 4°C overnight with anti-MUC4 mouse monoclonal antibody (1:100 in PBS, ab60720; Abcam). .. Cells were washed 3–5 times for 10 min with PBS containing 0.05% Tween 20 (PBS-T) and then incubated with cyanine 3–labeled goat anti-mouse secondary antibodies (#P0193; Beyotime) at 37°C for 60 min.

Transferring:

Article Title: Abnormal alterations in the Ca2+/CaV1.2/calmodulin/caMKII signaling pathway in a tremor rat model and in cultured hippocampal neurons exposed to Mg2+-free solution
Article Snippet: .. The pipette solution contained 50 mM K-Aspartate, 20 mM KCl, 20 mM HEPES, 1 mM EGTA, 1 mM MgCl2 , 0.2 mM CaCl2 , 13.6 mM NaCl and 3 mM K2ATP3 (pH 7.4; KOH; Beyotime Institute of Biotechnology). .. Patch-clamp electrodes were obtained with capillary tubes pulled with a P-97 puller (Gibco-BRL).

Size-exclusion Chromatography:

Article Title: Correlation between the expression of IL-18 and deep venous thrombosis
Article Snippet: For packaging and infection of the viral vectors, the pCDH-GFP lentivirus (12 g) was packaged with the pCL-ECO plasmid (8 g) and was transfected into the overexpression plasmid IL-18-pCDH-GFP using HEPES and the Calcium Phosphate Cell Transfection kit (Beyotime Institute of Biotechnology, Jiangsu, China) according to the manufacturer's protocols Normal cells and cells transfected with null vectors were used as controls. .. The target plasmid and packaging plasmid were added to 1.5 ml EP tubes in sequence and centrifuged at 300 × g for 10 sec at room temperature.

Blocking Assay:

Article Title: Specific-detection of clinical samples, systematic functional investigations, and transcriptome analysis reveals that splice variant MUC4/Y contributes to the malignant progression of pancreatic cancer by triggering malignancy-related positive feedback loops signaling
Article Snippet: .. Immunofluorescence Cells cultured to 70% confluence were washed with 0.1 mol/L HEPES containing Hanks’ buffer and fixed with Immunol Staining Fix Solution (#P0098; Beyotime) at room temperature for 15 min. After three 10-min washes in Immunol Staining Wash Buffer (#P0106; Beyotime), the fixed cells were blocked in Immunol Staining Blocking Buffer (#P0102; Beyotime) for 60 min at room temperature for nonspecific blocking, followed by incubation at 4°C overnight with anti-MUC4 mouse monoclonal antibody (1:100 in PBS, ab60720; Abcam). .. Cells were washed 3–5 times for 10 min with PBS containing 0.05% Tween 20 (PBS-T) and then incubated with cyanine 3–labeled goat anti-mouse secondary antibodies (#P0193; Beyotime) at 37°C for 60 min.

Incubation:

Article Title: Antibacterial Effect and Mode of Action of Flavonoids From Licorice Against Methicillin-Resistant Staphylococcus aureus
Article Snippet: .. Reactive Oxygen Species (ROS) Assay S. aureus ATCC29213 cells were washed three times with buffer containing 5 mM HEPES and 20 mM glucose at pH 7.2, adjusted to obtain a bacterial suspension with densities equivalent to a 0.5 McFarland turbidity standard, and then incubated with 10 μM dichloro-dihydro-fluorescein diacetate (DCFH-DA; Beyotime, Shanghai, China) at 37°C for 30 min. After incubation, the S. aureus cells were treated with different concentrations of drugs for 60 min, and the fluorescence intensity of the DCFH-DA dye was monitored using a Tecan Infinite 200 Pro Microplate Reader at an excitation wavelength of 488 nm and an emission wavelength of 525 nm ( ). .. Confocal Microscopy Assay S. aureus ATCC29213 cells were washed three times with PBS and then incubated with glabrol and lysostaphin for 30 min. After incubation, bacterial cells were washed with PBS and then stained with 5 μM PI and 10 μM SYTOTM 9 Green Fluorescent Nucleic Acid Stain (SYTO 9; Thermo Fisher Scientific, Waltham, MA, United States).

Article Title: Specific-detection of clinical samples, systematic functional investigations, and transcriptome analysis reveals that splice variant MUC4/Y contributes to the malignant progression of pancreatic cancer by triggering malignancy-related positive feedback loops signaling
Article Snippet: .. Immunofluorescence Cells cultured to 70% confluence were washed with 0.1 mol/L HEPES containing Hanks’ buffer and fixed with Immunol Staining Fix Solution (#P0098; Beyotime) at room temperature for 15 min. After three 10-min washes in Immunol Staining Wash Buffer (#P0106; Beyotime), the fixed cells were blocked in Immunol Staining Blocking Buffer (#P0102; Beyotime) for 60 min at room temperature for nonspecific blocking, followed by incubation at 4°C overnight with anti-MUC4 mouse monoclonal antibody (1:100 in PBS, ab60720; Abcam). .. Cells were washed 3–5 times for 10 min with PBS containing 0.05% Tween 20 (PBS-T) and then incubated with cyanine 3–labeled goat anti-mouse secondary antibodies (#P0193; Beyotime) at 37°C for 60 min.

Inhibition:

Article Title: Correlation between the expression of IL-18 and deep venous thrombosis
Article Snippet: Paragraph title: Acquisition of plasmid virus with IL-18 overexpression and inhibition, and efficiency determination ... For packaging and infection of the viral vectors, the pCDH-GFP lentivirus (12 g) was packaged with the pCL-ECO plasmid (8 g) and was transfected into the overexpression plasmid IL-18-pCDH-GFP using HEPES and the Calcium Phosphate Cell Transfection kit (Beyotime Institute of Biotechnology, Jiangsu, China) according to the manufacturer's protocols Normal cells and cells transfected with null vectors were used as controls.

Activity Assay:

Article Title: High Mobility Group Protein 1 Reverses Immune System Paralysis in Late-Phase Sepsis
Article Snippet: RPMI 1640 medium, fetal calf serum (FCS), glutamine, penicillin, streptomycin, and HEPES were purchased from Beyotime Biotech Co. Ltd., Shanghai, China. .. Polymyxin B was added to the cell culture medium at 10 μg/ml to neutralize the activity of endotoxin.

Infection:

Article Title: Correlation between the expression of IL-18 and deep venous thrombosis
Article Snippet: .. For packaging and infection of the viral vectors, the pCDH-GFP lentivirus (12 g) was packaged with the pCL-ECO plasmid (8 g) and was transfected into the overexpression plasmid IL-18-pCDH-GFP using HEPES and the Calcium Phosphate Cell Transfection kit (Beyotime Institute of Biotechnology, Jiangsu, China) according to the manufacturer's protocols Normal cells and cells transfected with null vectors were used as controls. ..

Inverted Microscopy:

Article Title: Abnormal alterations in the Ca2+/CaV1.2/calmodulin/caMKII signaling pathway in a tremor rat model and in cultured hippocampal neurons exposed to Mg2+-free solution
Article Snippet: The pipette solution contained 50 mM K-Aspartate, 20 mM KCl, 20 mM HEPES, 1 mM EGTA, 1 mM MgCl2 , 0.2 mM CaCl2 , 13.6 mM NaCl and 3 mM K2ATP3 (pH 7.4; KOH; Beyotime Institute of Biotechnology). .. To record action potential, cultures, including the normal cultured neurons and the neurons treated with Mg2+ -free solution, were transferred to the stage of an inverted microscope (TMS-1015, Olympus, Tokyo, Japan).

Staining:

Article Title: Specific-detection of clinical samples, systematic functional investigations, and transcriptome analysis reveals that splice variant MUC4/Y contributes to the malignant progression of pancreatic cancer by triggering malignancy-related positive feedback loops signaling
Article Snippet: .. Immunofluorescence Cells cultured to 70% confluence were washed with 0.1 mol/L HEPES containing Hanks’ buffer and fixed with Immunol Staining Fix Solution (#P0098; Beyotime) at room temperature for 15 min. After three 10-min washes in Immunol Staining Wash Buffer (#P0106; Beyotime), the fixed cells were blocked in Immunol Staining Blocking Buffer (#P0102; Beyotime) for 60 min at room temperature for nonspecific blocking, followed by incubation at 4°C overnight with anti-MUC4 mouse monoclonal antibody (1:100 in PBS, ab60720; Abcam). .. Cells were washed 3–5 times for 10 min with PBS containing 0.05% Tween 20 (PBS-T) and then incubated with cyanine 3–labeled goat anti-mouse secondary antibodies (#P0193; Beyotime) at 37°C for 60 min.

Sequencing:

Article Title: Correlation between the expression of IL-18 and deep venous thrombosis
Article Snippet: For packaging and infection of the viral vectors, the pCDH-GFP lentivirus (12 g) was packaged with the pCL-ECO plasmid (8 g) and was transfected into the overexpression plasmid IL-18-pCDH-GFP using HEPES and the Calcium Phosphate Cell Transfection kit (Beyotime Institute of Biotechnology, Jiangsu, China) according to the manufacturer's protocols Normal cells and cells transfected with null vectors were used as controls. .. The target plasmid and packaging plasmid were added to 1.5 ml EP tubes in sequence and centrifuged at 300 × g for 10 sec at room temperature.

Western Blot:

Article Title: Effects of vaspin on pancreatic β cell secretion via PI3K/Akt and NF-κB signaling pathways
Article Snippet: .. Penicillin-streptomycin, HEPES, sodium pyruvate, β-mercaptoethanol, Enhanced Chemiluminescence (ECL) western blot detection system were purchased from Beyotime (Shanghai, China). .. Recombinant human vaspin was from Phoenix (San Francisco, CA, USA).

Recombinant:

Article Title: Effects of vaspin on pancreatic β cell secretion via PI3K/Akt and NF-κB signaling pathways
Article Snippet: Penicillin-streptomycin, HEPES, sodium pyruvate, β-mercaptoethanol, Enhanced Chemiluminescence (ECL) western blot detection system were purchased from Beyotime (Shanghai, China). .. Recombinant human vaspin was from Phoenix (San Francisco, CA, USA).

BIA-KA:

Article Title: Effects of vaspin on pancreatic β cell secretion via PI3K/Akt and NF-κB signaling pathways
Article Snippet: Penicillin-streptomycin, HEPES, sodium pyruvate, β-mercaptoethanol, Enhanced Chemiluminescence (ECL) western blot detection system were purchased from Beyotime (Shanghai, China). .. Total RNA Extractor (Trizol) kit, the M-MLV RTase cDNA kit, RIPA lysate and the BCA protein assay kit were obtained from Boster (Wuhan, China).

Immunofluorescence:

Article Title: Specific-detection of clinical samples, systematic functional investigations, and transcriptome analysis reveals that splice variant MUC4/Y contributes to the malignant progression of pancreatic cancer by triggering malignancy-related positive feedback loops signaling
Article Snippet: .. Immunofluorescence Cells cultured to 70% confluence were washed with 0.1 mol/L HEPES containing Hanks’ buffer and fixed with Immunol Staining Fix Solution (#P0098; Beyotime) at room temperature for 15 min. After three 10-min washes in Immunol Staining Wash Buffer (#P0106; Beyotime), the fixed cells were blocked in Immunol Staining Blocking Buffer (#P0102; Beyotime) for 60 min at room temperature for nonspecific blocking, followed by incubation at 4°C overnight with anti-MUC4 mouse monoclonal antibody (1:100 in PBS, ab60720; Abcam). .. Cells were washed 3–5 times for 10 min with PBS containing 0.05% Tween 20 (PBS-T) and then incubated with cyanine 3–labeled goat anti-mouse secondary antibodies (#P0193; Beyotime) at 37°C for 60 min.

Plasmid Preparation:

Article Title: Correlation between the expression of IL-18 and deep venous thrombosis
Article Snippet: .. For packaging and infection of the viral vectors, the pCDH-GFP lentivirus (12 g) was packaged with the pCL-ECO plasmid (8 g) and was transfected into the overexpression plasmid IL-18-pCDH-GFP using HEPES and the Calcium Phosphate Cell Transfection kit (Beyotime Institute of Biotechnology, Jiangsu, China) according to the manufacturer's protocols Normal cells and cells transfected with null vectors were used as controls. ..

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    Beyotime hepes
    Hepes, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes/product/Beyotime
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hepes - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    97
    Beyotime hepes buffer solution
    Hepes Buffer Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes buffer solution/product/Beyotime
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hepes buffer solution - by Bioz Stars, 2020-03
    97/100 stars
      Buy from Supplier

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