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Atlanta Biologicals hepes
Hepes, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 98/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 4 article reviews
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hepes - by Bioz Stars, 2020-03
98/100 stars

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Cell Isolation:

Article Title: Transient cellular adhesion on poly(ethylene-glycol)-dimethacrylate hydrogels facilitates a novel stem cell bandage approach
Article Snippet: Paragraph title: Stem cell isolation ... The isolated stromal cells were then cultured in 89% Dulbecco’s Modification Of Eagle’s Medium/ Ham’s F-12 50/50 mix with L-glutamine & 15mM HEPES (DMEM/F-12; Atlanta Biologicals), 10% Fetal bovine serum (FBS; Atlanta Biologicals), and 1% penicillin streptomycin solution (Pen/Strep; Corning), and incubated at 25°C and 5% CO2 .

Article Title: Foxp2 Inhibits Nkx2.1-Mediated Transcription of SP-C via Interactions with the Nkx2.1 Homeodomain
Article Snippet: Paragraph title: Cell Isolation and Preparation of AEC Monolayers ... MLE-15 cells (J. Whitsett, University of Cincinnati [38]) were cultivated in HITES medium ((RPMI 1640 medium; Invitrogen, Carlsbad, CA) supplemented with 10 nM hydrocortisone, 5 μg/ml insulin, 5 μg/ml human transferrin, 10 nM β-estradiol, 5 μg/ml selenium, 2 mM L-glutamine, 10 mM HEPES, 100 U/ml penicillin, 100 μg/ml streptomycin; and 4% fetal bovine serum (FBS) (Atlanta Biologicals, Lawrenceville, GA)).

Luciferase:

Article Title: Cholesterol Side-Chain Cleavage Gene Expression in Theca Cells: Augmented Transcriptional Regulation and mRNA Stability in Polycystic Ovary Syndrome
Article Snippet: One hour prior to transfection, the cells were transferred into transfection medium containing, DME high-glucose medium containing 20 mM HEPES and 2% heat-inactivated calf serum (Atlanta Biologicals, Atlanta, GA), and moved to a 3% CO2 , 95% ambient air, 37°C incubator. .. The cells were then transfected with calcium phosphate precipitate containing 2 µg/dish of luciferase plasmid and 0.1 µg/dish of an expression vector for β-galactosidase, pSVβ-gal (Promega, Madison, WI) and 20 mM CaCl2 , per 30 mm well, as indicated.

Stable Transfection:

Article Title: Novel allosteric agonists of M1 muscarinic acetylcholine receptors induce brain region-specific responses that correspond with behavioral effects in animal models
Article Snippet: To generate a tetracycline (TET)-inducible human M1 mAChR stable cell line, TREx CHO cells expressing tet a repressor (Invitrogen) were transfected with human M1 -pcDNA5/TO expression plasmid, and subsequently went under hygromycin (450 μg/ml) selection in the presence of blasticidin (10 μg/ml) for maintaining tet repressors. .. TREx CHO cell line was cultured in the medium containing 10% Tetacycline-tested FBS, 10 mM Hepes (Atlanta Biologicals).

Cytometry:

Article Title: Enhancement of hematopoiesis and lymphopoiesis in diet-induced obese mice
Article Snippet: Bone marrow was harvested from both femurs of each mouse into Harvest Buffer (HBSS; Invitrogen), 10 mM Hepes, 4 mM sodium bicarbonate, and 4% heat-inactivated FBS (pH 7.2; Atlanta Biologicals) as described previously ( ). .. After red blood cell lysis, bone marrow cells were resuspended in Label Buffer (HBSS, 10 mM Hepes, 4 mM sodium bicarbonate, 2% heat-inactivated FBS, 0.15% sodium azide, pH 7.2) for counting and flow cytometry analysis.

Incubation:

Article Title: Development of Inhibitor-Directed Enzyme Prodrug Therapy (IDEPT) for Prostate Cancer
Article Snippet: Complete HyClone classical medium consisted of HyClone RPMI 1640 (Thermo Scientific, Rockford, IL) containing 2.05 mM l -glutamine and 25 mM Hepes, supplemented with 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA), 1× Penicillin–Streptomycin–Glutamine (Gibco, Life Technologies Corp., Grand Island, NY, 1 U/mL penicillin, 1 μg/mL streptomycin, 292 μg/mL l -glutamine final), and 1× sodium pyruvate (Gibco, Life Technologies Corp., Grand Island, NY, 1 mM final). .. After adherence for 48 h (roughly 70% confluency), media was removed (via inversion of plates), and cells were incubated with the IDEPT agent (0, 0.1, or 0.5 μM for LNCaP cells; 0, 0.5, or 1 μM for PC-3 cells) for 2.5 h, with mild agitation every 30 min.

Article Title: Transient cellular adhesion on poly(ethylene-glycol)-dimethacrylate hydrogels facilitates a novel stem cell bandage approach
Article Snippet: .. The isolated stromal cells were then cultured in 89% Dulbecco’s Modification Of Eagle’s Medium/ Ham’s F-12 50/50 mix with L-glutamine & 15mM HEPES (DMEM/F-12; Atlanta Biologicals), 10% Fetal bovine serum (FBS; Atlanta Biologicals), and 1% penicillin streptomycin solution (Pen/Strep; Corning), and incubated at 25°C and 5% CO2 . ..

Activity Assay:

Article Title: Cholesterol Side-Chain Cleavage Gene Expression in Theca Cells: Augmented Transcriptional Regulation and mRNA Stability in Polycystic Ovary Syndrome
Article Snippet: One hour prior to transfection, the cells were transferred into transfection medium containing, DME high-glucose medium containing 20 mM HEPES and 2% heat-inactivated calf serum (Atlanta Biologicals, Atlanta, GA), and moved to a 3% CO2 , 95% ambient air, 37°C incubator. .. Luciferase activity was determined with the Luciferase Assay System (Promega) on a Sirius Luminometer (Zylux Corp., Oak Ridge, TN). β-Galactosidase activity was measured by the chemiluminescent assay Galacto-Light Plus (Tropix, Bedford, MA) and utilized for normalization of transfection efficiency.

Expressing:

Article Title: Novel allosteric agonists of M1 muscarinic acetylcholine receptors induce brain region-specific responses that correspond with behavioral effects in animal models
Article Snippet: To generate a tetracycline (TET)-inducible human M1 mAChR stable cell line, TREx CHO cells expressing tet a repressor (Invitrogen) were transfected with human M1 -pcDNA5/TO expression plasmid, and subsequently went under hygromycin (450 μg/ml) selection in the presence of blasticidin (10 μg/ml) for maintaining tet repressors. .. TREx CHO cell line was cultured in the medium containing 10% Tetacycline-tested FBS, 10 mM Hepes (Atlanta Biologicals).

Article Title: Cholesterol Side-Chain Cleavage Gene Expression in Theca Cells: Augmented Transcriptional Regulation and mRNA Stability in Polycystic Ovary Syndrome
Article Snippet: One hour prior to transfection, the cells were transferred into transfection medium containing, DME high-glucose medium containing 20 mM HEPES and 2% heat-inactivated calf serum (Atlanta Biologicals, Atlanta, GA), and moved to a 3% CO2 , 95% ambient air, 37°C incubator. .. The cells were then transfected with calcium phosphate precipitate containing 2 µg/dish of luciferase plasmid and 0.1 µg/dish of an expression vector for β-galactosidase, pSVβ-gal (Promega, Madison, WI) and 20 mM CaCl2 , per 30 mm well, as indicated.

Article Title: Stimulation of B lymphocytes by cmvIL-10 but not LAcmvIL-10
Article Snippet: Daudi human B cell lymphoblastoma cells were obtained from American Type Culture Collection (ATCC, Manassas, VA) and maintained in RPMI 1640 tissue culture medium (Mediatech, Inc., Herndon, VA) supplemented with 4.5 g/L glucose, 1.5 g/L sodium bicarbonate, 2 mM L-glutamine, 1 mM sodium pyruvate, 10 mM HEPES and 10% fetal bovine serum (FBS; Atlanta Biologicals, Atlanta, GA) in a 37°C incubator with a humidified 5% CO2 environment. .. LAcmvIL-10 and cmvIL-10 were purified by ion-exchange chromatography from lysates of bacterial cells expressing plasmid encoded histidine-tagged cytokine.

Modification:

Article Title: Transient cellular adhesion on poly(ethylene-glycol)-dimethacrylate hydrogels facilitates a novel stem cell bandage approach
Article Snippet: .. The isolated stromal cells were then cultured in 89% Dulbecco’s Modification Of Eagle’s Medium/ Ham’s F-12 50/50 mix with L-glutamine & 15mM HEPES (DMEM/F-12; Atlanta Biologicals), 10% Fetal bovine serum (FBS; Atlanta Biologicals), and 1% penicillin streptomycin solution (Pen/Strep; Corning), and incubated at 25°C and 5% CO2 . ..

Article Title: Foxp2 Inhibits Nkx2.1-Mediated Transcription of SP-C via Interactions with the Nkx2.1 Homeodomain
Article Snippet: Freshly isolated AT2 cells were plated in minimal defined serum-free medium (MDSF) consisting of Dulbecco's modified Eagle's medium and Ham's F12 nutrient mixture in a 1:1 ratio (DME-F12), supplemented with 1.25 mg/ml bovine serum albumin (BSA), 10 mM HEPES, 0.1 mM nonessential amino acids, 2.0 mM L-glutamine, 100 U/ml sodium penicillin G, and 100 μg/ml streptomycin. .. MLE-15 cells (J. Whitsett, University of Cincinnati [38]) were cultivated in HITES medium ((RPMI 1640 medium; Invitrogen, Carlsbad, CA) supplemented with 10 nM hydrocortisone, 5 μg/ml insulin, 5 μg/ml human transferrin, 10 nM β-estradiol, 5 μg/ml selenium, 2 mM L-glutamine, 10 mM HEPES, 100 U/ml penicillin, 100 μg/ml streptomycin; and 4% fetal bovine serum (FBS) (Atlanta Biologicals, Lawrenceville, GA)).

Article Title: Cholesterol Side-Chain Cleavage Gene Expression in Theca Cells: Augmented Transcriptional Regulation and mRNA Stability in Polycystic Ovary Syndrome
Article Snippet: Transient transfection of normal and PCOS theca cells Human theca cells isolated from normal cycling women and women with PCOS were transfected as previously described , , using the modified calcium phosphate method of Graham and Van der Eb . .. One hour prior to transfection, the cells were transferred into transfection medium containing, DME high-glucose medium containing 20 mM HEPES and 2% heat-inactivated calf serum (Atlanta Biologicals, Atlanta, GA), and moved to a 3% CO2 , 95% ambient air, 37°C incubator.

Article Title: IQGAP1 Is a Novel CXCR2-Interacting Protein and Essential Component of the "Chemosynapse"
Article Snippet: Briefly, HL-60 cells were cultured in RPMI 1640 medium supplemented with 25 mM HEPES (pH 7.4), 10% fetal bovine serum (Atlanta Biologicals, Atlanta, GA), L-glutamine, 100 units/ml pen/strep (Mediatech, Inc., Herndon, VA). .. HEK293 cells were maintained in Dulbecco's Modified Eagle's Media with 10% fetal bovine serum and changed to serum free medium prior to assay.

Derivative Assay:

Article Title: Suppression of autophagy and antigen presentation by Mycobacterium tuberculosis PE_PGRS47
Article Snippet: Cells were seeded at 2 × 106 per 100 mm in bacteriological Petri dishes (VWR) in 10 ml of complete RPMI-1640 medium (Invitrogen) supplemented with 10 mM HEPES, 2 mM L-glutamine, 55 μM 2-mercaptoethanol, 50 μg ml−1 penicillin/streptomycin and 10% ultralow IgG heat-inactivated (56 °C, 30 min) fetal bovine serum (FBS; Atlanta Biologicals) and murine granulocyte-macrophage colony-stimulating factor (GM-CSF) (20 ng ml−1 ) (Peprotech). .. Bone marrow macrophages (BMMs) were derived according to previously published methods .

Transfection:

Article Title: Novel allosteric agonists of M1 muscarinic acetylcholine receptors induce brain region-specific responses that correspond with behavioral effects in animal models
Article Snippet: To generate a tetracycline (TET)-inducible human M1 mAChR stable cell line, TREx CHO cells expressing tet a repressor (Invitrogen) were transfected with human M1 -pcDNA5/TO expression plasmid, and subsequently went under hygromycin (450 μg/ml) selection in the presence of blasticidin (10 μg/ml) for maintaining tet repressors. .. TREx CHO cell line was cultured in the medium containing 10% Tetacycline-tested FBS, 10 mM Hepes (Atlanta Biologicals).

Article Title: Cholesterol Side-Chain Cleavage Gene Expression in Theca Cells: Augmented Transcriptional Regulation and mRNA Stability in Polycystic Ovary Syndrome
Article Snippet: .. One hour prior to transfection, the cells were transferred into transfection medium containing, DME high-glucose medium containing 20 mM HEPES and 2% heat-inactivated calf serum (Atlanta Biologicals, Atlanta, GA), and moved to a 3% CO2 , 95% ambient air, 37°C incubator. .. The cells were then transfected with calcium phosphate precipitate containing 2 µg/dish of luciferase plasmid and 0.1 µg/dish of an expression vector for β-galactosidase, pSVβ-gal (Promega, Madison, WI) and 20 mM CaCl2 , per 30 mm well, as indicated.

Buffer Exchange:

Article Title: Development of Inhibitor-Directed Enzyme Prodrug Therapy (IDEPT) for Prostate Cancer
Article Snippet: The recovered IDEPT agent was subjected to an additional buffer exchange step using an additional Zeba Spin Desalting Column. .. Complete HyClone classical medium consisted of HyClone RPMI 1640 (Thermo Scientific, Rockford, IL) containing 2.05 mM l -glutamine and 25 mM Hepes, supplemented with 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA), 1× Penicillin–Streptomycin–Glutamine (Gibco, Life Technologies Corp., Grand Island, NY, 1 U/mL penicillin, 1 μg/mL streptomycin, 292 μg/mL l -glutamine final), and 1× sodium pyruvate (Gibco, Life Technologies Corp., Grand Island, NY, 1 mM final).

Cell Culture:

Article Title: The Phagocytosis of Crystalline Silica Particles by Macrophages
Article Snippet: .. The mouse alveolar macrophage cell line MH-S (ATCC CRL-2019) and mouse peritoneal macrophage cell line RAW 264.7 (ATCC TIC-71) were cultured at 37°C with 5% CO2 in RPMI-1640 media (Media Tech, Pittsburgh, PA) supplemented with 2 mM L-glutamine, 1 mM sodium pyruvate, 10 mM HEPES, 55 μM 2-mercaptoethanol, 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA), 100 μg/ml ampicillin, and 100 μg/ml dihydrostreptomycin sulfate. .. Crystalline silica: Alpha-quartz particles (Min-U-Sil 5; US Silica, Berkeley Springs, WV) Spherical silica (Allsphere silica; Alltech Associates, Deerfield, IL).

Article Title: Novel allosteric agonists of M1 muscarinic acetylcholine receptors induce brain region-specific responses that correspond with behavioral effects in animal models
Article Snippet: .. TREx CHO cell line was cultured in the medium containing 10% Tetacycline-tested FBS, 10 mM Hepes (Atlanta Biologicals). .. For all calcium assays, humanM1 -TREx CHO or hM1 -CHO cells were seeded at a density of 50,000 cells/well in clear-bottomed, black-walled 96-well plates (Costar® Corning Incorporated, Corning, NY) in media containing a range of TET concentrations.

Article Title: Transient cellular adhesion on poly(ethylene-glycol)-dimethacrylate hydrogels facilitates a novel stem cell bandage approach
Article Snippet: .. The isolated stromal cells were then cultured in 89% Dulbecco’s Modification Of Eagle’s Medium/ Ham’s F-12 50/50 mix with L-glutamine & 15mM HEPES (DMEM/F-12; Atlanta Biologicals), 10% Fetal bovine serum (FBS; Atlanta Biologicals), and 1% penicillin streptomycin solution (Pen/Strep; Corning), and incubated at 25°C and 5% CO2 . ..

Article Title: Pro-Inflammatory Responses in Human Monocytes are ?1-Adrenergic Receptor Subtype Dependent a-Adrenergic Receptor Subtype Dependent a b
Article Snippet: .. A human monocytic cell line, THP-1 (ATCC, Manassas, VA) was propagated using standard cell culture conditions (37° C/5% CO2 ) in RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose and 10 mM HEPES (complete media), supplemented with 10% heat inactivated fetal bovine serum (FBS; Atlanta Biologicals, Lawrenceville, GA). .. Confluent THP-1 cells (106 cells/mL) were washed in serum-free complete medium, allowed to become quiescent for 30 min before pre-incubating with or without AR antagonists or inhibitors of PKA for 60 min prior to addition of the selective β-AR agonist, Iso (Sigma-Aldrich, St. Louis, MO) and/or an inflammatory receptor agonist, LPS (Sigma-Aldrich, St. Louis, MO).

Article Title: IQGAP1 Is a Novel CXCR2-Interacting Protein and Essential Component of the "Chemosynapse"
Article Snippet: .. Briefly, HL-60 cells were cultured in RPMI 1640 medium supplemented with 25 mM HEPES (pH 7.4), 10% fetal bovine serum (Atlanta Biologicals, Atlanta, GA), L-glutamine, 100 units/ml pen/strep (Mediatech, Inc., Herndon, VA). ..

Article Title: Vaccination Strategies to Enhance Local Immunity and Protection Against Mycobacterium tuberculosis
Article Snippet: .. Cells were cultured in complete medium, containing RPMI1640 (Invitrogen), 100 mM sodium pyruvate, 100 U/mL penicillin/streptomycin, 100 mM minimal non-essential amino acids, 10 mM HEPES, 50 μM 2-mercaptoethanol, and 10% heat inactivated fetal bovine serum (Atlanta Biologicals). .. Splenocytes were cultured in 24-well plates at 6 × 106 cells/well with either peptide CFP1071–90 (10 μg/ml), CFP10 (5 μg/ml), PPD (5 μg/ml) or in complete medium only as a negative control.

Article Title: Suppression of autophagy and antigen presentation by Mycobacterium tuberculosis PE_PGRS47
Article Snippet: Paragraph title: Primary cell culture and cell lines ... Cells were seeded at 2 × 106 per 100 mm in bacteriological Petri dishes (VWR) in 10 ml of complete RPMI-1640 medium (Invitrogen) supplemented with 10 mM HEPES, 2 mM L-glutamine, 55 μM 2-mercaptoethanol, 50 μg ml−1 penicillin/streptomycin and 10% ultralow IgG heat-inactivated (56 °C, 30 min) fetal bovine serum (FBS; Atlanta Biologicals) and murine granulocyte-macrophage colony-stimulating factor (GM-CSF) (20 ng ml−1 ) (Peprotech).

Article Title: Lysosomal Membrane Permeabilization is an Early Event in Sigma-2 Receptor Ligand Mediated Cell Death in Pancreatic Cancer
Article Snippet: .. Cell Culture Cell lines were maintained in RPMI media (GIBCO) supplemented with L-glutamine (2 mM), (HEPES) (1 mM), pyruvate (1 mM), sodium bicarbonate (0.075 % w/v), penicillin and streptomycin (100 IU/mL), amphotericin (0.25 μg/mL), and 10 % fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA). ..

other:

Article Title: Use of Positron Emission Tomography to Target Prostate Cancer Gene Therapy by Oncolytic Herpes Simplex Virus
Article Snippet: The androgen-sensitive prostate cancer cell line CWR22 (a kind gift of Dr. Zohar Dotan, Memorial Sloan Kettering Cancer Center (MSKCC), New York, NY) was grown in phenol red-free RPMI supplemented with 2mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate, 100 U/ml penicillin, 100 mg/ml streptomycin, and 10% charcoal dextran treated fetal bovine serum (Atlanta Biologicals, Norcross, GA).

Protein Concentration:

Article Title: Development of Inhibitor-Directed Enzyme Prodrug Therapy (IDEPT) for Prostate Cancer
Article Snippet: The recovered protein was then sterilized using a 0.22 μm syringe filter, and protein concentration was determined by the Bio-Rad Protein Assay (Bradford method) (Bio-Rad, Hercules, CA) using a bovine serum albumin standard. .. Complete HyClone classical medium consisted of HyClone RPMI 1640 (Thermo Scientific, Rockford, IL) containing 2.05 mM l -glutamine and 25 mM Hepes, supplemented with 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA), 1× Penicillin–Streptomycin–Glutamine (Gibco, Life Technologies Corp., Grand Island, NY, 1 U/mL penicillin, 1 μg/mL streptomycin, 292 μg/mL l -glutamine final), and 1× sodium pyruvate (Gibco, Life Technologies Corp., Grand Island, NY, 1 mM final).

Recombinant:

Article Title: Memory Responses in Human Immunodeficiency Virus Type 1-Infected Individuals with Long-Term Viral Load Suppression Are Independent of CD4 Cell Nadir
Article Snippet: The HIV-1 recombinant viral peptide gag p24 (IIIB) was obtained from Immunodiagnostics, Inc. (Woburn, Mass.). .. Complete medium consisted of RPMI with antibiotics, l -glutamine, minimal essential medium with nonessential amino acids, minimal essential medium with sodium pyruvate, and 10 mM HEPES plus 10% normal human serum, type AB+ (Atlanta Biologicals, Norcross, Ga.).

Article Title: Stimulation of B lymphocytes by cmvIL-10 but not LAcmvIL-10
Article Snippet: Daudi human B cell lymphoblastoma cells were obtained from American Type Culture Collection (ATCC, Manassas, VA) and maintained in RPMI 1640 tissue culture medium (Mediatech, Inc., Herndon, VA) supplemented with 4.5 g/L glucose, 1.5 g/L sodium bicarbonate, 2 mM L-glutamine, 1 mM sodium pyruvate, 10 mM HEPES and 10% fetal bovine serum (FBS; Atlanta Biologicals, Atlanta, GA) in a 37°C incubator with a humidified 5% CO2 environment. .. Purified recombinant human IL-4, IL-10 and cmvIL-10 were purchased from R & D Systems (Minneapolis, MN).

Crocin Bleaching Assay:

Article Title: Transient cellular adhesion on poly(ethylene-glycol)-dimethacrylate hydrogels facilitates a novel stem cell bandage approach
Article Snippet: The cells were resuspended in appropriate growth media and the live nucleated cells were counted on a Cellometer Vision CBA cell counter (Nexcelom Bioscience) using an AO/PI dye. .. The isolated stromal cells were then cultured in 89% Dulbecco’s Modification Of Eagle’s Medium/ Ham’s F-12 50/50 mix with L-glutamine & 15mM HEPES (DMEM/F-12; Atlanta Biologicals), 10% Fetal bovine serum (FBS; Atlanta Biologicals), and 1% penicillin streptomycin solution (Pen/Strep; Corning), and incubated at 25°C and 5% CO2 .

Ion Exchange Chromatography:

Article Title: Stimulation of B lymphocytes by cmvIL-10 but not LAcmvIL-10
Article Snippet: Daudi human B cell lymphoblastoma cells were obtained from American Type Culture Collection (ATCC, Manassas, VA) and maintained in RPMI 1640 tissue culture medium (Mediatech, Inc., Herndon, VA) supplemented with 4.5 g/L glucose, 1.5 g/L sodium bicarbonate, 2 mM L-glutamine, 1 mM sodium pyruvate, 10 mM HEPES and 10% fetal bovine serum (FBS; Atlanta Biologicals, Atlanta, GA) in a 37°C incubator with a humidified 5% CO2 environment. .. LAcmvIL-10 and cmvIL-10 were purified by ion-exchange chromatography from lysates of bacterial cells expressing plasmid encoded histidine-tagged cytokine.

Isolation:

Article Title: Transient cellular adhesion on poly(ethylene-glycol)-dimethacrylate hydrogels facilitates a novel stem cell bandage approach
Article Snippet: .. The isolated stromal cells were then cultured in 89% Dulbecco’s Modification Of Eagle’s Medium/ Ham’s F-12 50/50 mix with L-glutamine & 15mM HEPES (DMEM/F-12; Atlanta Biologicals), 10% Fetal bovine serum (FBS; Atlanta Biologicals), and 1% penicillin streptomycin solution (Pen/Strep; Corning), and incubated at 25°C and 5% CO2 . ..

Article Title: Foxp2 Inhibits Nkx2.1-Mediated Transcription of SP-C via Interactions with the Nkx2.1 Homeodomain
Article Snippet: AT2 cell purity ( > 90%) was assessed by staining freshly isolated cells with an antibody (Ab) to p180 lamellar membrane protein (Covance Research Products, Berkeley, CA). .. MLE-15 cells (J. Whitsett, University of Cincinnati [38]) were cultivated in HITES medium ((RPMI 1640 medium; Invitrogen, Carlsbad, CA) supplemented with 10 nM hydrocortisone, 5 μg/ml insulin, 5 μg/ml human transferrin, 10 nM β-estradiol, 5 μg/ml selenium, 2 mM L-glutamine, 10 mM HEPES, 100 U/ml penicillin, 100 μg/ml streptomycin; and 4% fetal bovine serum (FBS) (Atlanta Biologicals, Lawrenceville, GA)).

Article Title: Cholesterol Side-Chain Cleavage Gene Expression in Theca Cells: Augmented Transcriptional Regulation and mRNA Stability in Polycystic Ovary Syndrome
Article Snippet: Transient transfection of normal and PCOS theca cells Human theca cells isolated from normal cycling women and women with PCOS were transfected as previously described , , using the modified calcium phosphate method of Graham and Van der Eb . .. One hour prior to transfection, the cells were transferred into transfection medium containing, DME high-glucose medium containing 20 mM HEPES and 2% heat-inactivated calf serum (Atlanta Biologicals, Atlanta, GA), and moved to a 3% CO2 , 95% ambient air, 37°C incubator.

Article Title: Vaccination Strategies to Enhance Local Immunity and Protection Against Mycobacterium tuberculosis
Article Snippet: Paragraph title: Isolation of splenocytes and lung mononuclear cells ... Cells were cultured in complete medium, containing RPMI1640 (Invitrogen), 100 mM sodium pyruvate, 100 U/mL penicillin/streptomycin, 100 mM minimal non-essential amino acids, 10 mM HEPES, 50 μM 2-mercaptoethanol, and 10% heat inactivated fetal bovine serum (Atlanta Biologicals).

Flow Cytometry:

Article Title: Enhancement of hematopoiesis and lymphopoiesis in diet-induced obese mice
Article Snippet: Bone marrow was harvested from both femurs of each mouse into Harvest Buffer (HBSS; Invitrogen), 10 mM Hepes, 4 mM sodium bicarbonate, and 4% heat-inactivated FBS (pH 7.2; Atlanta Biologicals) as described previously ( ). .. After red blood cell lysis, bone marrow cells were resuspended in Label Buffer (HBSS, 10 mM Hepes, 4 mM sodium bicarbonate, 2% heat-inactivated FBS, 0.15% sodium azide, pH 7.2) for counting and flow cytometry analysis.

Purification:

Article Title: Stimulation of B lymphocytes by cmvIL-10 but not LAcmvIL-10
Article Snippet: Daudi human B cell lymphoblastoma cells were obtained from American Type Culture Collection (ATCC, Manassas, VA) and maintained in RPMI 1640 tissue culture medium (Mediatech, Inc., Herndon, VA) supplemented with 4.5 g/L glucose, 1.5 g/L sodium bicarbonate, 2 mM L-glutamine, 1 mM sodium pyruvate, 10 mM HEPES and 10% fetal bovine serum (FBS; Atlanta Biologicals, Atlanta, GA) in a 37°C incubator with a humidified 5% CO2 environment. .. Purified recombinant human IL-4, IL-10 and cmvIL-10 were purchased from R & D Systems (Minneapolis, MN).

Staining:

Article Title: Foxp2 Inhibits Nkx2.1-Mediated Transcription of SP-C via Interactions with the Nkx2.1 Homeodomain
Article Snippet: AT2 cell purity ( > 90%) was assessed by staining freshly isolated cells with an antibody (Ab) to p180 lamellar membrane protein (Covance Research Products, Berkeley, CA). .. MLE-15 cells (J. Whitsett, University of Cincinnati [38]) were cultivated in HITES medium ((RPMI 1640 medium; Invitrogen, Carlsbad, CA) supplemented with 10 nM hydrocortisone, 5 μg/ml insulin, 5 μg/ml human transferrin, 10 nM β-estradiol, 5 μg/ml selenium, 2 mM L-glutamine, 10 mM HEPES, 100 U/ml penicillin, 100 μg/ml streptomycin; and 4% fetal bovine serum (FBS) (Atlanta Biologicals, Lawrenceville, GA)).

Mouse Assay:

Article Title: Vaccination Strategies to Enhance Local Immunity and Protection Against Mycobacterium tuberculosis
Article Snippet: Mice were sacrificed, their organs were harvested and passed through a 40 μm strainer (BD Biosciences) followed by red blood cell lysis. .. Cells were cultured in complete medium, containing RPMI1640 (Invitrogen), 100 mM sodium pyruvate, 100 U/mL penicillin/streptomycin, 100 mM minimal non-essential amino acids, 10 mM HEPES, 50 μM 2-mercaptoethanol, and 10% heat inactivated fetal bovine serum (Atlanta Biologicals).

Article Title: Enhancement of hematopoiesis and lymphopoiesis in diet-induced obese mice
Article Snippet: Mice were anesthetized with isoflurane, blood was collected by cardiac puncture, and serum was collected for additional analysis. .. Bone marrow was harvested from both femurs of each mouse into Harvest Buffer (HBSS; Invitrogen), 10 mM Hepes, 4 mM sodium bicarbonate, and 4% heat-inactivated FBS (pH 7.2; Atlanta Biologicals) as described previously ( ).

Article Title: Suppression of autophagy and antigen presentation by Mycobacterium tuberculosis PE_PGRS47
Article Snippet: Briefly, bone marrow was obtained from tibiae and femurs of six- to eight-week-old female C57BL/6 mice. .. Cells were seeded at 2 × 106 per 100 mm in bacteriological Petri dishes (VWR) in 10 ml of complete RPMI-1640 medium (Invitrogen) supplemented with 10 mM HEPES, 2 mM L-glutamine, 55 μM 2-mercaptoethanol, 50 μg ml−1 penicillin/streptomycin and 10% ultralow IgG heat-inactivated (56 °C, 30 min) fetal bovine serum (FBS; Atlanta Biologicals) and murine granulocyte-macrophage colony-stimulating factor (GM-CSF) (20 ng ml−1 ) (Peprotech).

Plasmid Preparation:

Article Title: Novel allosteric agonists of M1 muscarinic acetylcholine receptors induce brain region-specific responses that correspond with behavioral effects in animal models
Article Snippet: To generate a tetracycline (TET)-inducible human M1 mAChR stable cell line, TREx CHO cells expressing tet a repressor (Invitrogen) were transfected with human M1 -pcDNA5/TO expression plasmid, and subsequently went under hygromycin (450 μg/ml) selection in the presence of blasticidin (10 μg/ml) for maintaining tet repressors. .. TREx CHO cell line was cultured in the medium containing 10% Tetacycline-tested FBS, 10 mM Hepes (Atlanta Biologicals).

Article Title: Cholesterol Side-Chain Cleavage Gene Expression in Theca Cells: Augmented Transcriptional Regulation and mRNA Stability in Polycystic Ovary Syndrome
Article Snippet: One hour prior to transfection, the cells were transferred into transfection medium containing, DME high-glucose medium containing 20 mM HEPES and 2% heat-inactivated calf serum (Atlanta Biologicals, Atlanta, GA), and moved to a 3% CO2 , 95% ambient air, 37°C incubator. .. The cells were then transfected with calcium phosphate precipitate containing 2 µg/dish of luciferase plasmid and 0.1 µg/dish of an expression vector for β-galactosidase, pSVβ-gal (Promega, Madison, WI) and 20 mM CaCl2 , per 30 mm well, as indicated.

Article Title: Stimulation of B lymphocytes by cmvIL-10 but not LAcmvIL-10
Article Snippet: Daudi human B cell lymphoblastoma cells were obtained from American Type Culture Collection (ATCC, Manassas, VA) and maintained in RPMI 1640 tissue culture medium (Mediatech, Inc., Herndon, VA) supplemented with 4.5 g/L glucose, 1.5 g/L sodium bicarbonate, 2 mM L-glutamine, 1 mM sodium pyruvate, 10 mM HEPES and 10% fetal bovine serum (FBS; Atlanta Biologicals, Atlanta, GA) in a 37°C incubator with a humidified 5% CO2 environment. .. LAcmvIL-10 and cmvIL-10 were purified by ion-exchange chromatography from lysates of bacterial cells expressing plasmid encoded histidine-tagged cytokine.

Selection:

Article Title: Novel allosteric agonists of M1 muscarinic acetylcholine receptors induce brain region-specific responses that correspond with behavioral effects in animal models
Article Snippet: The resulting hygromycin resistant polyclones were further plated into a 96 well plate for monoclonal selection. .. TREx CHO cell line was cultured in the medium containing 10% Tetacycline-tested FBS, 10 mM Hepes (Atlanta Biologicals).

In Vitro:

Article Title: Development of Inhibitor-Directed Enzyme Prodrug Therapy (IDEPT) for Prostate Cancer
Article Snippet: Paragraph title: In Vitro Cytotoxicity Assays ... Complete HyClone classical medium consisted of HyClone RPMI 1640 (Thermo Scientific, Rockford, IL) containing 2.05 mM l -glutamine and 25 mM Hepes, supplemented with 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA), 1× Penicillin–Streptomycin–Glutamine (Gibco, Life Technologies Corp., Grand Island, NY, 1 U/mL penicillin, 1 μg/mL streptomycin, 292 μg/mL l -glutamine final), and 1× sodium pyruvate (Gibco, Life Technologies Corp., Grand Island, NY, 1 mM final).

Lysis:

Article Title: Cholesterol Side-Chain Cleavage Gene Expression in Theca Cells: Augmented Transcriptional Regulation and mRNA Stability in Polycystic Ovary Syndrome
Article Snippet: One hour prior to transfection, the cells were transferred into transfection medium containing, DME high-glucose medium containing 20 mM HEPES and 2% heat-inactivated calf serum (Atlanta Biologicals, Atlanta, GA), and moved to a 3% CO2 , 95% ambient air, 37°C incubator. .. To increase transfection efficiency, 6 h following transfection, the cells were glycerol shocked with a solution containing 15% glycerol in 25 mM Hepes buffer containing 140 mM NaCl and 0.5 mM Na2 HPO4 , and transferred into transfection media with and without 20 µM forskolin for 48 h. The cells were then were harvested with trypsin, pelleted, and resuspended in reporter lysis buffer (Promega).

Article Title: Vaccination Strategies to Enhance Local Immunity and Protection Against Mycobacterium tuberculosis
Article Snippet: Mice were sacrificed, their organs were harvested and passed through a 40 μm strainer (BD Biosciences) followed by red blood cell lysis. .. Cells were cultured in complete medium, containing RPMI1640 (Invitrogen), 100 mM sodium pyruvate, 100 U/mL penicillin/streptomycin, 100 mM minimal non-essential amino acids, 10 mM HEPES, 50 μM 2-mercaptoethanol, and 10% heat inactivated fetal bovine serum (Atlanta Biologicals).

Article Title: Enhancement of hematopoiesis and lymphopoiesis in diet-induced obese mice
Article Snippet: Bone marrow was harvested from both femurs of each mouse into Harvest Buffer (HBSS; Invitrogen), 10 mM Hepes, 4 mM sodium bicarbonate, and 4% heat-inactivated FBS (pH 7.2; Atlanta Biologicals) as described previously ( ). .. After red blood cell lysis, bone marrow cells were resuspended in Label Buffer (HBSS, 10 mM Hepes, 4 mM sodium bicarbonate, 2% heat-inactivated FBS, 0.15% sodium azide, pH 7.2) for counting and flow cytometry analysis.

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  • 93
    Atlanta Biologicals hepes koh
    Doubling times of adult zebrafish liver cell line (ZFL) cells during adaptation to growth in UltraMEM™-ITES. ZFL cells were exchanged into decreasing percentages of L15-FBS and increasing percentages of UltraMEM™-ITES. Medium substitutions were made every second passage. Cell counts were taken every 2–8 days. Doubling times were calculated for ZFL growing in L15-FBS alone (blue diamonds), in L15-FBS supplemented with a range of UltraMEM™-ITES concentrations (red squares), or in UltraMEM™-ITES, <t>HEPES-KOH</t> supplemented with 2 mM taurine (green triangles).
    Hepes Koh, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes koh/product/Atlanta Biologicals
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hepes koh - by Bioz Stars, 2020-03
    93/100 stars
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    98
    Atlanta Biologicals hepes
    Doubling times of adult zebrafish liver cell line (ZFL) cells during adaptation to growth in UltraMEM™-ITES. ZFL cells were exchanged into decreasing percentages of L15-FBS and increasing percentages of UltraMEM™-ITES. Medium substitutions were made every second passage. Cell counts were taken every 2–8 days. Doubling times were calculated for ZFL growing in L15-FBS alone (blue diamonds), in L15-FBS supplemented with a range of UltraMEM™-ITES concentrations (red squares), or in UltraMEM™-ITES, <t>HEPES-KOH</t> supplemented with 2 mM taurine (green triangles).
    Hepes, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 98/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes/product/Atlanta Biologicals
    Average 98 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    hepes - by Bioz Stars, 2020-03
    98/100 stars
      Buy from Supplier

    91
    Atlanta Biologicals m hepes
    Doubling times of adult zebrafish liver cell line (ZFL) cells during adaptation to growth in UltraMEM™-ITES. ZFL cells were exchanged into decreasing percentages of L15-FBS and increasing percentages of UltraMEM™-ITES. Medium substitutions were made every second passage. Cell counts were taken every 2–8 days. Doubling times were calculated for ZFL growing in L15-FBS alone (blue diamonds), in L15-FBS supplemented with a range of UltraMEM™-ITES concentrations (red squares), or in UltraMEM™-ITES, <t>HEPES-KOH</t> supplemented with 2 mM taurine (green triangles).
    M Hepes, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m hepes/product/Atlanta Biologicals
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    m hepes - by Bioz Stars, 2020-03
    91/100 stars
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    Doubling times of adult zebrafish liver cell line (ZFL) cells during adaptation to growth in UltraMEM™-ITES. ZFL cells were exchanged into decreasing percentages of L15-FBS and increasing percentages of UltraMEM™-ITES. Medium substitutions were made every second passage. Cell counts were taken every 2–8 days. Doubling times were calculated for ZFL growing in L15-FBS alone (blue diamonds), in L15-FBS supplemented with a range of UltraMEM™-ITES concentrations (red squares), or in UltraMEM™-ITES, HEPES-KOH supplemented with 2 mM taurine (green triangles).

    Journal: Marine Drugs

    Article Title: Taurine Biosynthesis in a Fish Liver Cell Line (ZFL) Adapted to a Serum-Free Medium

    doi: 10.3390/md15060147

    Figure Lengend Snippet: Doubling times of adult zebrafish liver cell line (ZFL) cells during adaptation to growth in UltraMEM™-ITES. ZFL cells were exchanged into decreasing percentages of L15-FBS and increasing percentages of UltraMEM™-ITES. Medium substitutions were made every second passage. Cell counts were taken every 2–8 days. Doubling times were calculated for ZFL growing in L15-FBS alone (blue diamonds), in L15-FBS supplemented with a range of UltraMEM™-ITES concentrations (red squares), or in UltraMEM™-ITES, HEPES-KOH supplemented with 2 mM taurine (green triangles).

    Article Snippet: Cells were maintained in Leibovitz’s L-15 medium (Cellgro, Manassas, VA, USA), supplemented with 20 mM HEPES-KOH, pH 7, 2 mM sodium pyruvate, 100 units·mL−1 of penicillin and streptomycin, and supplemented with 9% fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA, USA) (L15-FBS) in 100 mm tissue culture plates at 28 °C, without sodium bicarbonate and CO2 ([ ]).

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