hepes cacl2  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 81

    Structured Review

    Millipore hepes cacl2
    Hepes Cacl2, supplied by Millipore, used in various techniques. Bioz Stars score: 81/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes cacl2/product/Millipore
    Average 81 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hepes cacl2 - by Bioz Stars, 2020-02
    81/100 stars

    Images

    Related Articles

    Flow Cytometry:

    Article Title: Post - prandial rise of microvesicles in peripheral blood of healthy human donors
    Article Snippet: For flow cytometric assessment of the number of MVs 70 μL of PBS - citrate was added to 30 μL of the isolate. .. To remove the unbound antibodies the stained isolates were washed by the addition of 100 μl of HEPES CaCl2 (17570 g, 37°C, 30 minutes, Sigma 3K30).

    Transfection:

    Article Title: Functional Characterization of Two scFv-Fc Antibodies from an HIV Controller Selected on Soluble HIV-1 Env Complexes: A Neutralizing V3- and a Trimer-Specific gp41 Antibody
    Article Snippet: .. Expression and Detection of Env on 293T Cells by FACS 293T cells were transfected with plasmids M118 encoding JR-FL Env and pRC-CMV-Rev1b (Alex Balazs, Caltech) encoding Rev in HEPES/CaCl2 (Sigma). .. Two days after transfection, cells were washed and resuspended in PBS.

    Concentration Assay:

    Article Title: Post - prandial rise of microvesicles in peripheral blood of healthy human donors
    Article Snippet: For determination of the cellular origin of MVs the isolate (30 μL) resuspended in HEPES CaCl2 buffer (100 μL) was incubated for 15 minutes in the dark at the room temperature with monoclonal antibodies against platelet (mouse anti human CD42b-PE IgG1, clone HIP1, ref. No. 555473, BD Biosciences, 2.5 μL), platelet - endothelial (mouse anti human CD31-FITC IgG1, clone WM59, ref. No. 555445, BD Biosciences, 5 μL) or erythrocyte (mouse anti human CD235-FITC IgG2b, clone GA-R2, BD Biosciences, 5 μL, diluted 1:10 v/v) surface molecules or concentration-matched isotype antibodies (PE Mouse IgG1, clone MOPC-21, ref. No. 555749; FITC Mouse IgG1, clone MOPC-21, ref. No. 555748; FITC Mouse IgG2b, clone 27-35; ref. No. 555742, BD Biosciences). .. To remove the unbound antibodies the stained isolates were washed by the addition of 100 μl of HEPES CaCl2 (17570 g, 37°C, 30 minutes, Sigma 3K30).

    Article Title: Functional Characterization of Two scFv-Fc Antibodies from an HIV Controller Selected on Soluble HIV-1 Env Complexes: A Neutralizing V3- and a Trimer-Specific gp41 Antibody
    Article Snippet: Expression and Detection of Env on 293T Cells by FACS 293T cells were transfected with plasmids M118 encoding JR-FL Env and pRC-CMV-Rev1b (Alex Balazs, Caltech) encoding Rev in HEPES/CaCl2 (Sigma). .. 1.2×105 cells were transfered to FACS vials, washed and stained with antibodies (final concentration 2.7 µg/mL) for 1 h in the dark at 4°C.

    Incubation:

    Article Title: Post - prandial rise of microvesicles in peripheral blood of healthy human donors
    Article Snippet: For determination of the cellular origin of MVs the isolate (30 μL) resuspended in HEPES CaCl2 buffer (100 μL) was incubated for 15 minutes in the dark at the room temperature with monoclonal antibodies against platelet (mouse anti human CD42b-PE IgG1, clone HIP1, ref. No. 555473, BD Biosciences, 2.5 μL), platelet - endothelial (mouse anti human CD31-FITC IgG1, clone WM59, ref. No. 555445, BD Biosciences, 5 μL) or erythrocyte (mouse anti human CD235-FITC IgG2b, clone GA-R2, BD Biosciences, 5 μL, diluted 1:10 v/v) surface molecules or concentration-matched isotype antibodies (PE Mouse IgG1, clone MOPC-21, ref. No. 555749; FITC Mouse IgG1, clone MOPC-21, ref. No. 555748; FITC Mouse IgG2b, clone 27-35; ref. No. 555742, BD Biosciences). .. To remove the unbound antibodies the stained isolates were washed by the addition of 100 μl of HEPES CaCl2 (17570 g, 37°C, 30 minutes, Sigma 3K30).

    Expressing:

    Article Title: Functional Characterization of Two scFv-Fc Antibodies from an HIV Controller Selected on Soluble HIV-1 Env Complexes: A Neutralizing V3- and a Trimer-Specific gp41 Antibody
    Article Snippet: .. Expression and Detection of Env on 293T Cells by FACS 293T cells were transfected with plasmids M118 encoding JR-FL Env and pRC-CMV-Rev1b (Alex Balazs, Caltech) encoding Rev in HEPES/CaCl2 (Sigma). .. Two days after transfection, cells were washed and resuspended in PBS.

    Staining:

    Article Title: Post - prandial rise of microvesicles in peripheral blood of healthy human donors
    Article Snippet: .. To remove the unbound antibodies the stained isolates were washed by the addition of 100 μl of HEPES CaCl2 (17570 g, 37°C, 30 minutes, Sigma 3K30). .. Flow cytometry analysis Analysis was performed by the Altra Flow Cytometer (Beckman Coulter Inc., Fullerton, CA).

    Article Title: Functional Characterization of Two scFv-Fc Antibodies from an HIV Controller Selected on Soluble HIV-1 Env Complexes: A Neutralizing V3- and a Trimer-Specific gp41 Antibody
    Article Snippet: Expression and Detection of Env on 293T Cells by FACS 293T cells were transfected with plasmids M118 encoding JR-FL Env and pRC-CMV-Rev1b (Alex Balazs, Caltech) encoding Rev in HEPES/CaCl2 (Sigma). .. 1.2×105 cells were transfered to FACS vials, washed and stained with antibodies (final concentration 2.7 µg/mL) for 1 h in the dark at 4°C.

    FACS:

    Article Title: Functional Characterization of Two scFv-Fc Antibodies from an HIV Controller Selected on Soluble HIV-1 Env Complexes: A Neutralizing V3- and a Trimer-Specific gp41 Antibody
    Article Snippet: .. Expression and Detection of Env on 293T Cells by FACS 293T cells were transfected with plasmids M118 encoding JR-FL Env and pRC-CMV-Rev1b (Alex Balazs, Caltech) encoding Rev in HEPES/CaCl2 (Sigma). .. Two days after transfection, cells were washed and resuspended in PBS.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Millipore annexin buffer
    Apoptosis, loss of ΔΨ m, and ROS production are caspase dependent. HeLa cells were treated with actinomycin D or UV (as indicated) in the presence or absence of 50 μM of zVAD-fmk, harvested, and stained with <t>annexin</t> V–FITC and PI to assess cell death, TMRE to measure ΔΨm, and 2-HE to measure ROS production, and then analyzed by flow cytometry.
    Annexin Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin buffer/product/Millipore
    Average 91 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    annexin buffer - by Bioz Stars, 2020-02
    91/100 stars
      Buy from Supplier

    78
    Millipore oocyte ringers hco3
    Apoptosis, loss of ΔΨ m, and ROS production are caspase dependent. HeLa cells were treated with actinomycin D or UV (as indicated) in the presence or absence of 50 μM of zVAD-fmk, harvested, and stained with <t>annexin</t> V–FITC and PI to assess cell death, TMRE to measure ΔΨm, and 2-HE to measure ROS production, and then analyzed by flow cytometry.
    Oocyte Ringers Hco3, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oocyte ringers hco3/product/Millipore
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    oocyte ringers hco3 - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    79
    Millipore lipolysis buffer
    Apoptosis, loss of ΔΨ m, and ROS production are caspase dependent. HeLa cells were treated with actinomycin D or UV (as indicated) in the presence or absence of 50 μM of zVAD-fmk, harvested, and stained with <t>annexin</t> V–FITC and PI to assess cell death, TMRE to measure ΔΨm, and 2-HE to measure ROS production, and then analyzed by flow cytometry.
    Lipolysis Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipolysis buffer/product/Millipore
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lipolysis buffer - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    80
    Millipore electrophysiology
    Apoptosis, loss of ΔΨ m, and ROS production are caspase dependent. HeLa cells were treated with actinomycin D or UV (as indicated) in the presence or absence of 50 μM of zVAD-fmk, harvested, and stained with <t>annexin</t> V–FITC and PI to assess cell death, TMRE to measure ΔΨm, and 2-HE to measure ROS production, and then analyzed by flow cytometry.
    Electrophysiology, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrophysiology/product/Millipore
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    electrophysiology - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    Image Search Results


    Apoptosis, loss of ΔΨ m, and ROS production are caspase dependent. HeLa cells were treated with actinomycin D or UV (as indicated) in the presence or absence of 50 μM of zVAD-fmk, harvested, and stained with annexin V–FITC and PI to assess cell death, TMRE to measure ΔΨm, and 2-HE to measure ROS production, and then analyzed by flow cytometry.

    Journal: The Journal of Cell Biology

    Article Title: Caspase-mediated loss of mitochondrial function and generation of reactive oxygen species during apoptosis

    doi: 10.1083/jcb.200208089

    Figure Lengend Snippet: Apoptosis, loss of ΔΨ m, and ROS production are caspase dependent. HeLa cells were treated with actinomycin D or UV (as indicated) in the presence or absence of 50 μM of zVAD-fmk, harvested, and stained with annexin V–FITC and PI to assess cell death, TMRE to measure ΔΨm, and 2-HE to measure ROS production, and then analyzed by flow cytometry.

    Article Snippet: The pellet was resuspended in 30 μl of annexin buffer (Hepes 10 mM, NaCl 150 mM, KCl 5 mM, MgCl2 1 mM, CaCl2 1.8 mM) and then divided into three groups: one third each for ΔΨm measurement (as described above), ROS measurement using 2 μM of 2-HE in MIB buffer, and cell death measurement using annexin V–FITC (Calbiochem).

    Techniques: Staining, Flow Cytometry, Cytometry