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hydroxyethyl 1 piperazineethanesulfonic acid buffer hepes diluted from 1 m gibco  (Thermo Fisher)


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    Thermo Fisher hydroxyethyl 1 piperazineethanesulfonic acid buffer hepes diluted from 1 m gibco
    Hydroxyethyl 1 Piperazineethanesulfonic Acid Buffer Hepes Diluted From 1 M Gibco, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hepes+buffer+1+m+gibco/HEPES%2C+1%2E0M+buffer+soln%2E%2C+pH+6%2E5/pm42248284-69-13-21
    Average 94 stars, based on 1 article reviews
    hydroxyethyl 1 piperazineethanesulfonic acid buffer hepes diluted from 1 m gibco - by Bioz Stars, 2026-09
    94/100 stars

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    Recombinant:

    Article Title: An ex vivo human placental explant model for controlled exposure studies.
    Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human placental tissue UCSF N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum Sigma-Aldrich Cat#F0926 Penicillin-streptomycin (10,000 U/mL) Gibco Cat#15140122 Gentamicin (50 mg/mL) Gibco Cat#15750060 DPBS Gibco Cat#14190250 DMEM, high glucose Gibco Cat#11965092 Glutamine plus (200 mM) Atlanta Biologicals Cat#B90210 Nutridoma (1003) Roche Cat#11011375001 Sodium pyruvate (100 mM) Gibco Cat#11360070 HEPES buffer (1 M) Gibco Cat15630080 SYBRTM Green qPCR Master Mix Applied Biosystems Cat#43-676-59 10% Neutral Buffered Formalin RPI Cat#F10800–500.0 Ethanol 200PRF Decon Labs Cat#V1001 Software and algorithms LAS Science Microscope Software Leica https://www.leica-microsystems.com Other 0.22 μm pore size, sterile vacuum filtration system, 500 mL bottle top – – 50 mL conical tube – – 1.5 mL microcentrifuge tube – – Petri dish (150, 100, and 35 mm) – – 24-well plate – – Serological pipette (5, 10, and 25 mL) – – Measuring cylinders – – Pipette gun – – Single-channel pipette and pipette tips (10, 200, and 1,000 μL) – – Surgical scissors – – Micro scissors – – Forceps – – Ruler – – Water bath – – Ice bucket – – Sterile CO 2 incubator – – 4 ◦ C Fridge – – Dissection microscope – – Cytowash Reagent Final concentration Amount DMEM, high glucose 95.4% 477 mL Fetal bovine serum 2.5% 12.5 mL (Continued on next page) 4 STAR Protocols 6, 104055, September 19, 2025 ..

    Real-time Polymerase Chain Reaction:

    Article Title: An ex vivo human placental explant model for controlled exposure studies.
    Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human placental tissue UCSF N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum Sigma-Aldrich Cat#F0926 Penicillin-streptomycin (10,000 U/mL) Gibco Cat#15140122 Gentamicin (50 mg/mL) Gibco Cat#15750060 DPBS Gibco Cat#14190250 DMEM, high glucose Gibco Cat#11965092 Glutamine plus (200 mM) Atlanta Biologicals Cat#B90210 Nutridoma (1003) Roche Cat#11011375001 Sodium pyruvate (100 mM) Gibco Cat#11360070 HEPES buffer (1 M) Gibco Cat15630080 SYBRTM Green qPCR Master Mix Applied Biosystems Cat#43-676-59 10% Neutral Buffered Formalin RPI Cat#F10800–500.0 Ethanol 200PRF Decon Labs Cat#V1001 Software and algorithms LAS Science Microscope Software Leica https://www.leica-microsystems.com Other 0.22 μm pore size, sterile vacuum filtration system, 500 mL bottle top – – 50 mL conical tube – – 1.5 mL microcentrifuge tube – – Petri dish (150, 100, and 35 mm) – – 24-well plate – – Serological pipette (5, 10, and 25 mL) – – Measuring cylinders – – Pipette gun – – Single-channel pipette and pipette tips (10, 200, and 1,000 μL) – – Surgical scissors – – Micro scissors – – Forceps – – Ruler – – Water bath – – Ice bucket – – Sterile CO 2 incubator – – 4 ◦ C Fridge – – Dissection microscope – – Cytowash Reagent Final concentration Amount DMEM, high glucose 95.4% 477 mL Fetal bovine serum 2.5% 12.5 mL (Continued on next page) 4 STAR Protocols 6, 104055, September 19, 2025 ..

    Software:

    Article Title: An ex vivo human placental explant model for controlled exposure studies.
    Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human placental tissue UCSF N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum Sigma-Aldrich Cat#F0926 Penicillin-streptomycin (10,000 U/mL) Gibco Cat#15140122 Gentamicin (50 mg/mL) Gibco Cat#15750060 DPBS Gibco Cat#14190250 DMEM, high glucose Gibco Cat#11965092 Glutamine plus (200 mM) Atlanta Biologicals Cat#B90210 Nutridoma (1003) Roche Cat#11011375001 Sodium pyruvate (100 mM) Gibco Cat#11360070 HEPES buffer (1 M) Gibco Cat15630080 SYBRTM Green qPCR Master Mix Applied Biosystems Cat#43-676-59 10% Neutral Buffered Formalin RPI Cat#F10800–500.0 Ethanol 200PRF Decon Labs Cat#V1001 Software and algorithms LAS Science Microscope Software Leica https://www.leica-microsystems.com Other 0.22 μm pore size, sterile vacuum filtration system, 500 mL bottle top – – 50 mL conical tube – – 1.5 mL microcentrifuge tube – – Petri dish (150, 100, and 35 mm) – – 24-well plate – – Serological pipette (5, 10, and 25 mL) – – Measuring cylinders – – Pipette gun – – Single-channel pipette and pipette tips (10, 200, and 1,000 μL) – – Surgical scissors – – Micro scissors – – Forceps – – Ruler – – Water bath – – Ice bucket – – Sterile CO 2 incubator – – 4 ◦ C Fridge – – Dissection microscope – – Cytowash Reagent Final concentration Amount DMEM, high glucose 95.4% 477 mL Fetal bovine serum 2.5% 12.5 mL (Continued on next page) 4 STAR Protocols 6, 104055, September 19, 2025 ..

    Microscopy:

    Article Title: An ex vivo human placental explant model for controlled exposure studies.
    Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human placental tissue UCSF N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum Sigma-Aldrich Cat#F0926 Penicillin-streptomycin (10,000 U/mL) Gibco Cat#15140122 Gentamicin (50 mg/mL) Gibco Cat#15750060 DPBS Gibco Cat#14190250 DMEM, high glucose Gibco Cat#11965092 Glutamine plus (200 mM) Atlanta Biologicals Cat#B90210 Nutridoma (1003) Roche Cat#11011375001 Sodium pyruvate (100 mM) Gibco Cat#11360070 HEPES buffer (1 M) Gibco Cat15630080 SYBRTM Green qPCR Master Mix Applied Biosystems Cat#43-676-59 10% Neutral Buffered Formalin RPI Cat#F10800–500.0 Ethanol 200PRF Decon Labs Cat#V1001 Software and algorithms LAS Science Microscope Software Leica https://www.leica-microsystems.com Other 0.22 μm pore size, sterile vacuum filtration system, 500 mL bottle top – – 50 mL conical tube – – 1.5 mL microcentrifuge tube – – Petri dish (150, 100, and 35 mm) – – 24-well plate – – Serological pipette (5, 10, and 25 mL) – – Measuring cylinders – – Pipette gun – – Single-channel pipette and pipette tips (10, 200, and 1,000 μL) – – Surgical scissors – – Micro scissors – – Forceps – – Ruler – – Water bath – – Ice bucket – – Sterile CO 2 incubator – – 4 ◦ C Fridge – – Dissection microscope – – Cytowash Reagent Final concentration Amount DMEM, high glucose 95.4% 477 mL Fetal bovine serum 2.5% 12.5 mL (Continued on next page) 4 STAR Protocols 6, 104055, September 19, 2025 ..

    Pore Size:

    Article Title: An ex vivo human placental explant model for controlled exposure studies.
    Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human placental tissue UCSF N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum Sigma-Aldrich Cat#F0926 Penicillin-streptomycin (10,000 U/mL) Gibco Cat#15140122 Gentamicin (50 mg/mL) Gibco Cat#15750060 DPBS Gibco Cat#14190250 DMEM, high glucose Gibco Cat#11965092 Glutamine plus (200 mM) Atlanta Biologicals Cat#B90210 Nutridoma (1003) Roche Cat#11011375001 Sodium pyruvate (100 mM) Gibco Cat#11360070 HEPES buffer (1 M) Gibco Cat15630080 SYBRTM Green qPCR Master Mix Applied Biosystems Cat#43-676-59 10% Neutral Buffered Formalin RPI Cat#F10800–500.0 Ethanol 200PRF Decon Labs Cat#V1001 Software and algorithms LAS Science Microscope Software Leica https://www.leica-microsystems.com Other 0.22 μm pore size, sterile vacuum filtration system, 500 mL bottle top – – 50 mL conical tube – – 1.5 mL microcentrifuge tube – – Petri dish (150, 100, and 35 mm) – – 24-well plate – – Serological pipette (5, 10, and 25 mL) – – Measuring cylinders – – Pipette gun – – Single-channel pipette and pipette tips (10, 200, and 1,000 μL) – – Surgical scissors – – Micro scissors – – Forceps – – Ruler – – Water bath – – Ice bucket – – Sterile CO 2 incubator – – 4 ◦ C Fridge – – Dissection microscope – – Cytowash Reagent Final concentration Amount DMEM, high glucose 95.4% 477 mL Fetal bovine serum 2.5% 12.5 mL (Continued on next page) 4 STAR Protocols 6, 104055, September 19, 2025 ..

    Sterility:

    Article Title: An ex vivo human placental explant model for controlled exposure studies.
    Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human placental tissue UCSF N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum Sigma-Aldrich Cat#F0926 Penicillin-streptomycin (10,000 U/mL) Gibco Cat#15140122 Gentamicin (50 mg/mL) Gibco Cat#15750060 DPBS Gibco Cat#14190250 DMEM, high glucose Gibco Cat#11965092 Glutamine plus (200 mM) Atlanta Biologicals Cat#B90210 Nutridoma (1003) Roche Cat#11011375001 Sodium pyruvate (100 mM) Gibco Cat#11360070 HEPES buffer (1 M) Gibco Cat15630080 SYBRTM Green qPCR Master Mix Applied Biosystems Cat#43-676-59 10% Neutral Buffered Formalin RPI Cat#F10800–500.0 Ethanol 200PRF Decon Labs Cat#V1001 Software and algorithms LAS Science Microscope Software Leica https://www.leica-microsystems.com Other 0.22 μm pore size, sterile vacuum filtration system, 500 mL bottle top – – 50 mL conical tube – – 1.5 mL microcentrifuge tube – – Petri dish (150, 100, and 35 mm) – – 24-well plate – – Serological pipette (5, 10, and 25 mL) – – Measuring cylinders – – Pipette gun – – Single-channel pipette and pipette tips (10, 200, and 1,000 μL) – – Surgical scissors – – Micro scissors – – Forceps – – Ruler – – Water bath – – Ice bucket – – Sterile CO 2 incubator – – 4 ◦ C Fridge – – Dissection microscope – – Cytowash Reagent Final concentration Amount DMEM, high glucose 95.4% 477 mL Fetal bovine serum 2.5% 12.5 mL (Continued on next page) 4 STAR Protocols 6, 104055, September 19, 2025 ..

    Suction Filtration:

    Article Title: An ex vivo human placental explant model for controlled exposure studies.
    Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human placental tissue UCSF N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum Sigma-Aldrich Cat#F0926 Penicillin-streptomycin (10,000 U/mL) Gibco Cat#15140122 Gentamicin (50 mg/mL) Gibco Cat#15750060 DPBS Gibco Cat#14190250 DMEM, high glucose Gibco Cat#11965092 Glutamine plus (200 mM) Atlanta Biologicals Cat#B90210 Nutridoma (1003) Roche Cat#11011375001 Sodium pyruvate (100 mM) Gibco Cat#11360070 HEPES buffer (1 M) Gibco Cat15630080 SYBRTM Green qPCR Master Mix Applied Biosystems Cat#43-676-59 10% Neutral Buffered Formalin RPI Cat#F10800–500.0 Ethanol 200PRF Decon Labs Cat#V1001 Software and algorithms LAS Science Microscope Software Leica https://www.leica-microsystems.com Other 0.22 μm pore size, sterile vacuum filtration system, 500 mL bottle top – – 50 mL conical tube – – 1.5 mL microcentrifuge tube – – Petri dish (150, 100, and 35 mm) – – 24-well plate – – Serological pipette (5, 10, and 25 mL) – – Measuring cylinders – – Pipette gun – – Single-channel pipette and pipette tips (10, 200, and 1,000 μL) – – Surgical scissors – – Micro scissors – – Forceps – – Ruler – – Water bath – – Ice bucket – – Sterile CO 2 incubator – – 4 ◦ C Fridge – – Dissection microscope – – Cytowash Reagent Final concentration Amount DMEM, high glucose 95.4% 477 mL Fetal bovine serum 2.5% 12.5 mL (Continued on next page) 4 STAR Protocols 6, 104055, September 19, 2025 ..

    Ubiquitin Proteomics:

    Article Title: An ex vivo human placental explant model for controlled exposure studies.
    Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human placental tissue UCSF N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum Sigma-Aldrich Cat#F0926 Penicillin-streptomycin (10,000 U/mL) Gibco Cat#15140122 Gentamicin (50 mg/mL) Gibco Cat#15750060 DPBS Gibco Cat#14190250 DMEM, high glucose Gibco Cat#11965092 Glutamine plus (200 mM) Atlanta Biologicals Cat#B90210 Nutridoma (1003) Roche Cat#11011375001 Sodium pyruvate (100 mM) Gibco Cat#11360070 HEPES buffer (1 M) Gibco Cat15630080 SYBRTM Green qPCR Master Mix Applied Biosystems Cat#43-676-59 10% Neutral Buffered Formalin RPI Cat#F10800–500.0 Ethanol 200PRF Decon Labs Cat#V1001 Software and algorithms LAS Science Microscope Software Leica https://www.leica-microsystems.com Other 0.22 μm pore size, sterile vacuum filtration system, 500 mL bottle top – – 50 mL conical tube – – 1.5 mL microcentrifuge tube – – Petri dish (150, 100, and 35 mm) – – 24-well plate – – Serological pipette (5, 10, and 25 mL) – – Measuring cylinders – – Pipette gun – – Single-channel pipette and pipette tips (10, 200, and 1,000 μL) – – Surgical scissors – – Micro scissors – – Forceps – – Ruler – – Water bath – – Ice bucket – – Sterile CO 2 incubator – – 4 ◦ C Fridge – – Dissection microscope – – Cytowash Reagent Final concentration Amount DMEM, high glucose 95.4% 477 mL Fetal bovine serum 2.5% 12.5 mL (Continued on next page) 4 STAR Protocols 6, 104055, September 19, 2025 ..

    Transferring:

    Article Title: An ex vivo human placental explant model for controlled exposure studies.
    Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human placental tissue UCSF N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum Sigma-Aldrich Cat#F0926 Penicillin-streptomycin (10,000 U/mL) Gibco Cat#15140122 Gentamicin (50 mg/mL) Gibco Cat#15750060 DPBS Gibco Cat#14190250 DMEM, high glucose Gibco Cat#11965092 Glutamine plus (200 mM) Atlanta Biologicals Cat#B90210 Nutridoma (1003) Roche Cat#11011375001 Sodium pyruvate (100 mM) Gibco Cat#11360070 HEPES buffer (1 M) Gibco Cat15630080 SYBRTM Green qPCR Master Mix Applied Biosystems Cat#43-676-59 10% Neutral Buffered Formalin RPI Cat#F10800–500.0 Ethanol 200PRF Decon Labs Cat#V1001 Software and algorithms LAS Science Microscope Software Leica https://www.leica-microsystems.com Other 0.22 μm pore size, sterile vacuum filtration system, 500 mL bottle top – – 50 mL conical tube – – 1.5 mL microcentrifuge tube – – Petri dish (150, 100, and 35 mm) – – 24-well plate – – Serological pipette (5, 10, and 25 mL) – – Measuring cylinders – – Pipette gun – – Single-channel pipette and pipette tips (10, 200, and 1,000 μL) – – Surgical scissors – – Micro scissors – – Forceps – – Ruler – – Water bath – – Ice bucket – – Sterile CO 2 incubator – – 4 ◦ C Fridge – – Dissection microscope – – Cytowash Reagent Final concentration Amount DMEM, high glucose 95.4% 477 mL Fetal bovine serum 2.5% 12.5 mL (Continued on next page) 4 STAR Protocols 6, 104055, September 19, 2025 ..

    Dissection:

    Article Title: An ex vivo human placental explant model for controlled exposure studies.
    Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human placental tissue UCSF N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum Sigma-Aldrich Cat#F0926 Penicillin-streptomycin (10,000 U/mL) Gibco Cat#15140122 Gentamicin (50 mg/mL) Gibco Cat#15750060 DPBS Gibco Cat#14190250 DMEM, high glucose Gibco Cat#11965092 Glutamine plus (200 mM) Atlanta Biologicals Cat#B90210 Nutridoma (1003) Roche Cat#11011375001 Sodium pyruvate (100 mM) Gibco Cat#11360070 HEPES buffer (1 M) Gibco Cat15630080 SYBRTM Green qPCR Master Mix Applied Biosystems Cat#43-676-59 10% Neutral Buffered Formalin RPI Cat#F10800–500.0 Ethanol 200PRF Decon Labs Cat#V1001 Software and algorithms LAS Science Microscope Software Leica https://www.leica-microsystems.com Other 0.22 μm pore size, sterile vacuum filtration system, 500 mL bottle top – – 50 mL conical tube – – 1.5 mL microcentrifuge tube – – Petri dish (150, 100, and 35 mm) – – 24-well plate – – Serological pipette (5, 10, and 25 mL) – – Measuring cylinders – – Pipette gun – – Single-channel pipette and pipette tips (10, 200, and 1,000 μL) – – Surgical scissors – – Micro scissors – – Forceps – – Ruler – – Water bath – – Ice bucket – – Sterile CO 2 incubator – – 4 ◦ C Fridge – – Dissection microscope – – Cytowash Reagent Final concentration Amount DMEM, high glucose 95.4% 477 mL Fetal bovine serum 2.5% 12.5 mL (Continued on next page) 4 STAR Protocols 6, 104055, September 19, 2025 ..

    Concentration Assay:

    Article Title: An ex vivo human placental explant model for controlled exposure studies.
    Article Snippet: .. MATERIALS AND EQUIPMENT REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human placental tissue UCSF N/A Chemicals, peptides, and recombinant proteins Fetal bovine serum Sigma-Aldrich Cat#F0926 Penicillin-streptomycin (10,000 U/mL) Gibco Cat#15140122 Gentamicin (50 mg/mL) Gibco Cat#15750060 DPBS Gibco Cat#14190250 DMEM, high glucose Gibco Cat#11965092 Glutamine plus (200 mM) Atlanta Biologicals Cat#B90210 Nutridoma (1003) Roche Cat#11011375001 Sodium pyruvate (100 mM) Gibco Cat#11360070 HEPES buffer (1 M) Gibco Cat15630080 SYBRTM Green qPCR Master Mix Applied Biosystems Cat#43-676-59 10% Neutral Buffered Formalin RPI Cat#F10800–500.0 Ethanol 200PRF Decon Labs Cat#V1001 Software and algorithms LAS Science Microscope Software Leica https://www.leica-microsystems.com Other 0.22 μm pore size, sterile vacuum filtration system, 500 mL bottle top – – 50 mL conical tube – – 1.5 mL microcentrifuge tube – – Petri dish (150, 100, and 35 mm) – – 24-well plate – – Serological pipette (5, 10, and 25 mL) – – Measuring cylinders – – Pipette gun – – Single-channel pipette and pipette tips (10, 200, and 1,000 μL) – – Surgical scissors – – Micro scissors – – Forceps – – Ruler – – Water bath – – Ice bucket – – Sterile CO 2 incubator – – 4 ◦ C Fridge – – Dissection microscope – – Cytowash Reagent Final concentration Amount DMEM, high glucose 95.4% 477 mL Fetal bovine serum 2.5% 12.5 mL (Continued on next page) 4 STAR Protocols 6, 104055, September 19, 2025 ..

    other:

    Article Title: Protocol for biomimetic tumoroid models by plastic compression using centrifugation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Sodium hydroxide Honeywell/Fluka Cat# 011-002-00-6 MEM (10x) Gibco Cat# 11570566 Collagen (rat tail) type I First Link Cat# 60-30-810 HEPES buffer (1 M) Gibco Cat# 15630-056 RPMI 1640 + L-glutamine Gibco Cat# 21875-034 Fetal bovine serum (FBS) Gibco Cat# 11550356 Penicillin-streptomycin solution Gibco Cat# 11548876 PBS Gibco Cat# 10010023 Trypsin-EDTA Gibco Cat# 25200056 Trypan blue Invitrogen Cat# 10702404 Critical commercial assays PrestoBlue cell viability reagent Invitrogen Cat# A13261 (Continued on next page) 2 STAR Protocols 6, 103718, June 20, 2025 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Raw and analyzed This paper N/A Experimental models: Cell lines MCF-7 ECACC Cat# 86012803 MDA-MB-231 ECACC Cat# 92020424 Software and algorithms ImageJ Open-source https://imagej.net/ij/download.html GraphPad Prism 10 Dotmatics https://www.graphpad.com/updates Other Centrifuge 5910 R Eppendorf Cat# 16622842 FreeZone 2.5 L 50 C benchtop freeze dryer Labconco Cat# 700202050 Kinexus Prime pro+ rheometer Netzsch N/A Zeiss Axio Observer Zeiss Apotome.2 Tecan plate reader Tecan Infinite M Plex 50 mL Falcon tubes Corning Cat# 430829 96-well culture plates Corning Cat# 3599 24-well culture plates Corning Cat# 3524 Surgical forceps DBIO GmbH Cat# DBF1001



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