DSMZ
helicobacter hepaticus infection Helicobacter Hepaticus Infection, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/helicobacter hepaticus infection/product/DSMZ Average 86 stars, based on 1 article reviews
helicobacter hepaticus infection - by Bioz Stars,
2025-02
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DSMZ
hepaticus Hepaticus, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/hepaticus/product/DSMZ Average 86 stars, based on 1 article reviews
hepaticus - by Bioz Stars,
2025-02
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Janvier Labs
helicobacter hepaticus Helicobacter Hepaticus, supplied by Janvier Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/helicobacter hepaticus/product/Janvier Labs Average 86 stars, based on 1 article reviews
helicobacter hepaticus - by Bioz Stars,
2025-02
86/100 stars
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DSMZ
helicobacter hepaticus Helicobacter Hepaticus, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/helicobacter hepaticus/product/DSMZ Average 86 stars, based on 1 article reviews
helicobacter hepaticus - by Bioz Stars,
2025-02
86/100 stars
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Corning Life Sciences
c hepaticus hv10 t ∆ pglb ![]() C Hepaticus Hv10 T ∆ Pglb, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/c hepaticus hv10 t ∆ pglb/product/Corning Life Sciences Average 86 stars, based on 1 article reviews
c hepaticus hv10 t ∆ pglb - by Bioz Stars,
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New England Biolabs
c hepaticus hv10 t wt ![]() C Hepaticus Hv10 T Wt, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/c hepaticus hv10 t wt/product/New England Biolabs Average 86 stars, based on 1 article reviews
c hepaticus hv10 t wt - by Bioz Stars,
2025-02
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Journal: mBio
Article Title: Development of tools for the genetic manipulation of Campylobacter and their application to the N- glycosylation system of Campylobacter hepaticus, an emerging pathogen of poultry
doi: 10.1128/mbio.01101-24
Figure Lengend Snippet: The genetic organization of C. hepaticus HV10 T protein N -glycosylation locus . Open reading frames predicted to be involved in sugar biosynthesis are illustrated in green, flippase in orange, glycosyltransferases in yellow, oligosaccharyltransferase in black, and pglG in white, as it has no known function in the C. jejuni N- glycosylation pathway . The red arrow represents the predicted putative promoter of galE .
Article Snippet: A 96-well High Bind Microplate (
Techniques:
Journal: mBio
Article Title: Development of tools for the genetic manipulation of Campylobacter and their application to the N- glycosylation system of Campylobacter hepaticus, an emerging pathogen of poultry
doi: 10.1128/mbio.01101-24
Figure Lengend Snippet: Efficiency of electro-transformation of C. hepaticus and C. bilis strains with shuttle vector pJBM3 isolated from E. coli NEB 5-alpha or C. hepaticus HV10 T
Article Snippet: A 96-well High Bind Microplate (
Techniques: Plasmid Preparation, Isolation, Transformation Assay
Journal: mBio
Article Title: Development of tools for the genetic manipulation of Campylobacter and their application to the N- glycosylation system of Campylobacter hepaticus, an emerging pathogen of poultry
doi: 10.1128/mbio.01101-24
Figure Lengend Snippet: Schematic diagram of the construction of pglB mutants by homologous recombination. Relevant chromosomal genes are shown. The diagram illustrates the process by which the wild-type (WT) pglB gene from C. hepaticus HV10 T was deleted by replacement with the aph(3’)-IIIa on pCH_ pglB _SV by a double crossover recombination event utilizing 1,103 and 1,120 bp homologous left and right flanking regions (blue) of pglB, respectively. The primers used to screen for double crossover events are provided in purple on the C. hepaticus HV10 T ∆ pglB::kan chromosome.
Article Snippet: A 96-well High Bind Microplate (
Techniques: Homologous Recombination
Journal: mBio
Article Title: Development of tools for the genetic manipulation of Campylobacter and their application to the N- glycosylation system of Campylobacter hepaticus, an emerging pathogen of poultry
doi: 10.1128/mbio.01101-24
Figure Lengend Snippet: The effect of pglB mutagenesis on SBA binding profiles to N- glycans present in C. hepaticus HV10 T ∆ pglB::kan mutants. Mutant 3 was independently derived from mutants 7, 8, 9, and 10. ( A ) Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and ( B ) SBA lectin blotting of C. hepaticus HV10 T (WT) and C. hepaticus HV10 T ∆ pglB::kan mutants of whole cell lysates containing 35–40 μg of protein. Whole cell lysates were separated by 8%–16% SDS-PAGE and either developed with SimplyBlue SafeStain or transferred to PVDF membranes for lectin blotting.
Article Snippet: A 96-well High Bind Microplate (
Techniques: Mutagenesis, Binding Assay, Derivative Assay, Polyacrylamide Gel Electrophoresis, SDS Page
Journal: mBio
Article Title: Development of tools for the genetic manipulation of Campylobacter and their application to the N- glycosylation system of Campylobacter hepaticus, an emerging pathogen of poultry
doi: 10.1128/mbio.01101-24
Figure Lengend Snippet: Plasmid map of shuttle vector pJBM5.2 used to complement C. hepaticus HV10 T ∆ pglB::kan mutant 7. Genetic symbols represent: Rep, replication initiation protein; HP, hypothetical protein; Mob, mobilization protein; OriV, Campylobacter origin of replication; ampR, Ampicillin resistance gene; ori, E.coli origin of replication from pJBM5; galE putative promoter; pglB, pglA, pglC, pglD, pglE, and pglF from C. hepaticus HV10 T ; and tet(O ), tetracycline resistance gene from C. hepaticus 84B. The position of the primers used to construct pJBM5.2 by Gibson assembly is shown in purple.
Article Snippet: A 96-well High Bind Microplate (
Techniques: Plasmid Preparation, Mutagenesis, Construct
Journal: mBio
Article Title: Development of tools for the genetic manipulation of Campylobacter and their application to the N- glycosylation system of Campylobacter hepaticus, an emerging pathogen of poultry
doi: 10.1128/mbio.01101-24
Figure Lengend Snippet: Complementation of C. hepaticus HV10 T ∆ pglB::kan mutant 7 in trans with pJBM5.2, expressing the pgl locus genes including and downstream of pglB and excluding pglG . ( A ) SBA lectin blot binding profiles to N- glycans present in C. hepaticus HV10 T , C. hepaticus HV10 T ∆ pglB::kan mutant 7, C. hepaticus HV10 T ∆ pglB::kan mutant 7(pJBM4) (empty vector control), and two clones from separate isogenic complements (clones 1 and 2 were derived independently of clones 3 and 4) of C. hepaticus HV10 T ∆ pglB::kan mutant 7 clones expressing functional pgl genes encoded on pJBM5.2. Whole cell lysates contained 27 µg of protein and were separated by 4%–20% SDS-PAGE and either developed with SimplyBlue SafeStain or transferred to PVDF membranes for lectin blotting. The corresponding SDS-PAGE gel to demonstrate equal loading of proteins across samples is provided in Fig. S8. ( B ) Quantitative analysis of SBA binding to GalNAc present in whole cell lysates. ELISA results represent SBA binding to whole cell lysates of C. hepaticus HV10 T , C. hepaticus HV10 T ∆ pglB::kan mutant 7, C. hepaticus HV10 T ∆ pglB::kan mutant 7(pJBM4), C. hepaticus HV10 T ∆ pglB::kan mutant 7(pJBM5.2), and Salmonella Typhimurium 82/6915 ∆ aroA (STM1) (negative control for N -glycosylation) measured at OD 450 . Gray bars represent the standard deviation of n = 4 technical replicates for each strain. Statistical differences between groups were analyzed using a one-way ANOVA with Tukey’s post hoc analysis and are indicated as: ns, no statistically significant difference ( P -value > 0.05); and **** indicate statistically significant differences with P -values < 0.0001. For all Tukey’s multiple comparisons, see Table S1.
Article Snippet: A 96-well High Bind Microplate (
Techniques: Mutagenesis, Expressing, Binding Assay, Plasmid Preparation, Control, Clone Assay, Derivative Assay, Functional Assay, SDS Page, Enzyme-linked Immunosorbent Assay, Negative Control, Standard Deviation
Journal: mBio
Article Title: Development of tools for the genetic manipulation of Campylobacter and their application to the N- glycosylation system of Campylobacter hepaticus, an emerging pathogen of poultry
doi: 10.1128/mbio.01101-24
Figure Lengend Snippet: Bacterial strains and plasmids used in this study
Article Snippet: A 96-well High Bind Microplate (
Techniques: Plasmid Preparation, Mutagenesis, Clone Assay, Isolation
Journal: mBio
Article Title: Development of tools for the genetic manipulation of Campylobacter and their application to the N- glycosylation system of Campylobacter hepaticus, an emerging pathogen of poultry
doi: 10.1128/mbio.01101-24
Figure Lengend Snippet: The genetic organization of C. hepaticus HV10 T protein N -glycosylation locus . Open reading frames predicted to be involved in sugar biosynthesis are illustrated in green, flippase in orange, glycosyltransferases in yellow, oligosaccharyltransferase in black, and pglG in white, as it has no known function in the C. jejuni N- glycosylation pathway . The red arrow represents the predicted putative promoter of galE .
Article Snippet: Genomic DNA was isolated from
Techniques:
Journal: mBio
Article Title: Development of tools for the genetic manipulation of Campylobacter and their application to the N- glycosylation system of Campylobacter hepaticus, an emerging pathogen of poultry
doi: 10.1128/mbio.01101-24
Figure Lengend Snippet: Efficiency of electro-transformation of C. hepaticus and C. bilis strains with shuttle vector pJBM3 isolated from E. coli NEB 5-alpha or C. hepaticus HV10 T
Article Snippet: Genomic DNA was isolated from
Techniques: Plasmid Preparation, Isolation, Transformation Assay
Journal: mBio
Article Title: Development of tools for the genetic manipulation of Campylobacter and their application to the N- glycosylation system of Campylobacter hepaticus, an emerging pathogen of poultry
doi: 10.1128/mbio.01101-24
Figure Lengend Snippet: Schematic diagram of the construction of pglB mutants by homologous recombination. Relevant chromosomal genes are shown. The diagram illustrates the process by which the wild-type (WT) pglB gene from C. hepaticus HV10 T was deleted by replacement with the aph(3’)-IIIa on pCH_ pglB _SV by a double crossover recombination event utilizing 1,103 and 1,120 bp homologous left and right flanking regions (blue) of pglB, respectively. The primers used to screen for double crossover events are provided in purple on the C. hepaticus HV10 T ∆ pglB::kan chromosome.
Article Snippet: Genomic DNA was isolated from
Techniques: Homologous Recombination
Journal: mBio
Article Title: Development of tools for the genetic manipulation of Campylobacter and their application to the N- glycosylation system of Campylobacter hepaticus, an emerging pathogen of poultry
doi: 10.1128/mbio.01101-24
Figure Lengend Snippet: The effect of pglB mutagenesis on SBA binding profiles to N- glycans present in C. hepaticus HV10 T ∆ pglB::kan mutants. Mutant 3 was independently derived from mutants 7, 8, 9, and 10. ( A ) Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and ( B ) SBA lectin blotting of C. hepaticus HV10 T (WT) and C. hepaticus HV10 T ∆ pglB::kan mutants of whole cell lysates containing 35–40 μg of protein. Whole cell lysates were separated by 8%–16% SDS-PAGE and either developed with SimplyBlue SafeStain or transferred to PVDF membranes for lectin blotting.
Article Snippet: Genomic DNA was isolated from
Techniques: Mutagenesis, Binding Assay, Derivative Assay, Polyacrylamide Gel Electrophoresis, SDS Page
Journal: mBio
Article Title: Development of tools for the genetic manipulation of Campylobacter and their application to the N- glycosylation system of Campylobacter hepaticus, an emerging pathogen of poultry
doi: 10.1128/mbio.01101-24
Figure Lengend Snippet: Plasmid map of shuttle vector pJBM5.2 used to complement C. hepaticus HV10 T ∆ pglB::kan mutant 7. Genetic symbols represent: Rep, replication initiation protein; HP, hypothetical protein; Mob, mobilization protein; OriV, Campylobacter origin of replication; ampR, Ampicillin resistance gene; ori, E.coli origin of replication from pJBM5; galE putative promoter; pglB, pglA, pglC, pglD, pglE, and pglF from C. hepaticus HV10 T ; and tet(O ), tetracycline resistance gene from C. hepaticus 84B. The position of the primers used to construct pJBM5.2 by Gibson assembly is shown in purple.
Article Snippet: Genomic DNA was isolated from
Techniques: Plasmid Preparation, Mutagenesis, Construct
Journal: mBio
Article Title: Development of tools for the genetic manipulation of Campylobacter and their application to the N- glycosylation system of Campylobacter hepaticus, an emerging pathogen of poultry
doi: 10.1128/mbio.01101-24
Figure Lengend Snippet: Complementation of C. hepaticus HV10 T ∆ pglB::kan mutant 7 in trans with pJBM5.2, expressing the pgl locus genes including and downstream of pglB and excluding pglG . ( A ) SBA lectin blot binding profiles to N- glycans present in C. hepaticus HV10 T , C. hepaticus HV10 T ∆ pglB::kan mutant 7, C. hepaticus HV10 T ∆ pglB::kan mutant 7(pJBM4) (empty vector control), and two clones from separate isogenic complements (clones 1 and 2 were derived independently of clones 3 and 4) of C. hepaticus HV10 T ∆ pglB::kan mutant 7 clones expressing functional pgl genes encoded on pJBM5.2. Whole cell lysates contained 27 µg of protein and were separated by 4%–20% SDS-PAGE and either developed with SimplyBlue SafeStain or transferred to PVDF membranes for lectin blotting. The corresponding SDS-PAGE gel to demonstrate equal loading of proteins across samples is provided in Fig. S8. ( B ) Quantitative analysis of SBA binding to GalNAc present in whole cell lysates. ELISA results represent SBA binding to whole cell lysates of C. hepaticus HV10 T , C. hepaticus HV10 T ∆ pglB::kan mutant 7, C. hepaticus HV10 T ∆ pglB::kan mutant 7(pJBM4), C. hepaticus HV10 T ∆ pglB::kan mutant 7(pJBM5.2), and Salmonella Typhimurium 82/6915 ∆ aroA (STM1) (negative control for N -glycosylation) measured at OD 450 . Gray bars represent the standard deviation of n = 4 technical replicates for each strain. Statistical differences between groups were analyzed using a one-way ANOVA with Tukey’s post hoc analysis and are indicated as: ns, no statistically significant difference ( P -value > 0.05); and **** indicate statistically significant differences with P -values < 0.0001. For all Tukey’s multiple comparisons, see Table S1.
Article Snippet: Genomic DNA was isolated from
Techniques: Mutagenesis, Expressing, Binding Assay, Plasmid Preparation, Control, Clone Assay, Derivative Assay, Functional Assay, SDS Page, Enzyme-linked Immunosorbent Assay, Negative Control, Standard Deviation
Journal: mBio
Article Title: Development of tools for the genetic manipulation of Campylobacter and their application to the N- glycosylation system of Campylobacter hepaticus, an emerging pathogen of poultry
doi: 10.1128/mbio.01101-24
Figure Lengend Snippet: Bacterial strains and plasmids used in this study
Article Snippet: Genomic DNA was isolated from
Techniques: Plasmid Preparation, Mutagenesis, Clone Assay, Isolation