Structured Review

Biacore heparan sulfate
A-B-A SPR competition analysis of binding of glycans to NHBA. Competition of: ( i) chondroitin sulfate D disaccharide (CS) with heparin (H) or <t>heparan</t> sulfate (HS); ( ii) H with CS or HS; (iii) HS with CS or H. Glycans were used at concentration 10x their respective K D s, and injected in the following order: glycan 1 (A) → glycan 1 and glycan 2 (B) → glycan 1 (A). PBS is used as a control.
Heparan Sulfate, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "The Bexsero Neisseria meningitidis serogroup B vaccine antigen NHBA is a high-affinity chondroitin sulfate binding protein"

Article Title: The Bexsero Neisseria meningitidis serogroup B vaccine antigen NHBA is a high-affinity chondroitin sulfate binding protein

Journal: Scientific Reports

doi: 10.1038/s41598-018-24639-x

A-B-A SPR competition analysis of binding of glycans to NHBA. Competition of: ( i) chondroitin sulfate D disaccharide (CS) with heparin (H) or heparan sulfate (HS); ( ii) H with CS or HS; (iii) HS with CS or H. Glycans were used at concentration 10x their respective K D s, and injected in the following order: glycan 1 (A) → glycan 1 and glycan 2 (B) → glycan 1 (A). PBS is used as a control.
Figure Legend Snippet: A-B-A SPR competition analysis of binding of glycans to NHBA. Competition of: ( i) chondroitin sulfate D disaccharide (CS) with heparin (H) or heparan sulfate (HS); ( ii) H with CS or HS; (iii) HS with CS or H. Glycans were used at concentration 10x their respective K D s, and injected in the following order: glycan 1 (A) → glycan 1 and glycan 2 (B) → glycan 1 (A). PBS is used as a control.

Techniques Used: SPR Assay, Binding Assay, Concentration Assay, Injection

SPR analysis of NHBA interactions with heparan sulfate, heparin and heparin disaccharides. Heparin and heparan sulfate polymers display complex, non-uniform sulfation patterns. The structures provided are representative of the major repeat units. #S: Sulfation at the # carbon. NS: No-sulfation on the specified monomer.
Figure Legend Snippet: SPR analysis of NHBA interactions with heparan sulfate, heparin and heparin disaccharides. Heparin and heparan sulfate polymers display complex, non-uniform sulfation patterns. The structures provided are representative of the major repeat units. #S: Sulfation at the # carbon. NS: No-sulfation on the specified monomer.

Techniques Used: SPR Assay

2) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

3) Product Images from "Host cell heparan sulfate glycosaminoglycans are ligands for OspF-related proteins of the Lyme disease spirochete"

Article Title: Host cell heparan sulfate glycosaminoglycans are ligands for OspF-related proteins of the Lyme disease spirochete

Journal: Cellular microbiology

doi: 10.1111/cmi.12448

B. burgdorferi producing ErpG binds to heparin and heparan sulfate
Figure Legend Snippet: B. burgdorferi producing ErpG binds to heparin and heparan sulfate

Techniques Used:

OspF-related proteins bind to heparan sulfate
Figure Legend Snippet: OspF-related proteins bind to heparan sulfate

Techniques Used:

Binding of ErpG-producing B. burgdorferi strain B314 to C6 glial cells is mediated by heparan sulfate
Figure Legend Snippet: Binding of ErpG-producing B. burgdorferi strain B314 to C6 glial cells is mediated by heparan sulfate

Techniques Used: Binding Assay

Recombinant ErpG binds to heparin and heparan sulfate
Figure Legend Snippet: Recombinant ErpG binds to heparin and heparan sulfate

Techniques Used: Recombinant

Recombinant ErpG proteins bind to heparan sulfate
Figure Legend Snippet: Recombinant ErpG proteins bind to heparan sulfate

Techniques Used: Recombinant

4) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

5) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

6) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

7) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

8) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

9) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

10) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

11) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

12) Product Images from "Insights into the role of sulfated glycans in cancer cell adhesion and migration through use of branched peptide probe"

Article Title: Insights into the role of sulfated glycans in cancer cell adhesion and migration through use of branched peptide probe

Journal: Scientific Reports

doi: 10.1038/srep27174

Flow cytometry of NT4 binding (grey) to PANC-1 cancer cells and inhibition by heparin (oblique lines), heparan sulfate (horizontal lines) and chondroitin sulfate (line).
Figure Legend Snippet: Flow cytometry of NT4 binding (grey) to PANC-1 cancer cells and inhibition by heparin (oblique lines), heparan sulfate (horizontal lines) and chondroitin sulfate (line).

Techniques Used: Flow Cytometry, Cytometry, Binding Assay, Inhibition

Biacore analysis of NT4-GAGs binding and affinity. ( A ) Sensorgrams of GAGs (10 μg/mL) binding to surface immobilized NT4. ( B–D ) Heparin ( B ), heparan sulfate ( C ) and chondroitin sulfate ( D ) binding to NT4. Different concentrations of GAGs were injected over biotin-conjugated NT4, previously immobilized on a SA sensor chip. ( E) Kinetics constants and affinity values of GAGs binding to NT4 peptide.
Figure Legend Snippet: Biacore analysis of NT4-GAGs binding and affinity. ( A ) Sensorgrams of GAGs (10 μg/mL) binding to surface immobilized NT4. ( B–D ) Heparin ( B ), heparan sulfate ( C ) and chondroitin sulfate ( D ) binding to NT4. Different concentrations of GAGs were injected over biotin-conjugated NT4, previously immobilized on a SA sensor chip. ( E) Kinetics constants and affinity values of GAGs binding to NT4 peptide.

Techniques Used: Binding Assay, Injection, Chromatin Immunoprecipitation

13) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

14) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

15) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

16) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

17) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

18) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

19) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

20) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

21) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

22) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

23) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

24) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

25) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

26) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

27) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

28) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

29) Product Images from "Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *"

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

Journal:

doi: 10.1074/jbc.M109.002840

A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
Figure Legend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

Techniques Used: SPR Assay, Binding Assay, Injection

Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin
Figure Legend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

Techniques Used:

Related Articles

Inhibition:

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *
Article Snippet: .. The binding of the truncated α5β1 ectodomain was abolished by heparin (100% inhibition) and partially inhibited (26%) by chondroitin sulfate and heparan sulfate (20%), whereas carboxymethyl dextran did not inhibit significantly the interaction (10%). ..

other:

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *
Article Snippet: The direct interaction between integrins and heparan sulfate at the cell surface might thus promote endostatin localization to lipid rafts.

Binding Assay:

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *
Article Snippet: .. The binding of the truncated α5β1 ectodomain was abolished by heparin (100% inhibition) and partially inhibited (26%) by chondroitin sulfate and heparan sulfate (20%), whereas carboxymethyl dextran did not inhibit significantly the interaction (10%). ..

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *
Article Snippet: .. Several arginine residues of endostatin (Arg24 , Arg53 , and Arg139 ) participate in the binding to the αvβ3 integrin in both models and to heparan sulfate ( , ). .. These results suggest that the αvβ3 integrin and heparan sulfate bind to the same site, or to substantially overlapped sites, on endostatin and that endostatin is not able to bind simultaneously to the αvβ3 integrin and heparan sulfate.

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *
Article Snippet: .. All of these data will contribute to a better understanding of the mechanism of endostatin binding to integrins and heparan sulfate at the surface of endothelial cells. ..

Article Title: The Bexsero Neisseria meningitidis serogroup B vaccine antigen NHBA is a high-affinity chondroitin sulfate binding protein
Article Snippet: .. Competition between chondroitin sulfate, heparan sulfate and heparin for NHBA binding To investigate the nature of binding sites on NHBA, a competition SPR assay was performed with the three main GAG substrates of NHBA using the A-B-A function of the Biacore S200 software. ..

Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *
Article Snippet: .. Although it seemed unlikely that endostatin could bind simultaneously to integrins and to heparan sulfate because Arg27 and Arg139 residues participate in the binding of endostatin to both integrins and heparan sulfate, we tried to determine whether heparin was able to inhibit the binding of integrins to endostatin. .. Interestingly, it turned out by SPR binding assays that α5β1 ( A ), αvβ3, and αvβ5 integrins (data not shown) also bound to immobilized heparin or heparan sulfate by SPR binding assays.

SPR Assay:

Article Title: Host cell heparan sulfate glycosaminoglycans are ligands for OspF-related proteins of the Lyme disease spirochete
Article Snippet: .. Interactions of Erp proteins with heparan sulfate were analyzed by a SPR technique using a Biacore 3000 (GE Healthcare, Piscataway, NJ). .. Ten μg of biotinylated heparan sulfate was conjugated to an SA chip (GE Healthcare, Piscataway, NJ).

Article Title: The Bexsero Neisseria meningitidis serogroup B vaccine antigen NHBA is a high-affinity chondroitin sulfate binding protein
Article Snippet: .. Competition between chondroitin sulfate, heparan sulfate and heparin for NHBA binding To investigate the nature of binding sites on NHBA, a competition SPR assay was performed with the three main GAG substrates of NHBA using the A-B-A function of the Biacore S200 software. ..

Software:

Article Title: The Bexsero Neisseria meningitidis serogroup B vaccine antigen NHBA is a high-affinity chondroitin sulfate binding protein
Article Snippet: .. Competition between chondroitin sulfate, heparan sulfate and heparin for NHBA binding To investigate the nature of binding sites on NHBA, a competition SPR assay was performed with the three main GAG substrates of NHBA using the A-B-A function of the Biacore S200 software. ..

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  • 88
    Biacore hspg fgf2 fgfr1 complex formation
    Effect of Ac-ARPCA-NH 2 on the <t>HSPG/FGF2/FGFR1</t> complex and FGFR signaling. (A) HSPG-deficient, FGFR1-transfected CHO cells were added to wild-type CHO-K1 monolayers in serum-free medium with FGF2 (1.66 nM) in the presence of Ac-ARPCA-NH 2 (d) or Ac-ARP S A-NH 2 (○) peptides. After 2 hrs of incubation at 37°C, the cells bound to the monolayer were counted under an inverted microscope. Experiments were performed in triplicate and were repeated twice with similar results. (B) GM7373 cells were treated with vehicle (a), 0.17 nM FGF2 (b), or FGF2 plus Ac-ARPCA-NH 2 (c) or Ac-ARP S A-NH 2 (•) peptides (both at 1.0 μM). After 10 min., cell extracts were analysed by Western blotting using anti-phospho-ERK 1/2 and anti-ERK 2 antibodies. (C) Sensogram overlay showing the binding of FGF2 (150 nM or 50 nM, respectively) to heparin-coated ( upper panel ) or sFGFR1(IIIc)/Fc chimera-coated ( lower panel ) BIAcore sensor chips in the absence ( _____ ) or in the presence of Ac-ARPCA-NH 2 (- - -) or Ac-ARP S A-NH 2 ( …… ) peptides (both at 1.0 μM). The response (in RU) was recorded as a function of time.
    Hspg Fgf2 Fgfr1 Complex Formation, supplied by Biacore, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Biacore heparan sulfate
    A-B-A SPR competition analysis of binding of glycans to NHBA. Competition of: ( i) chondroitin sulfate D disaccharide (CS) with heparin (H) or <t>heparan</t> sulfate (HS); ( ii) H with CS or HS; (iii) HS with CS or H. Glycans were used at concentration 10x their respective K D s, and injected in the following order: glycan 1 (A) → glycan 1 and glycan 2 (B) → glycan 1 (A). PBS is used as a control.
    Heparan Sulfate, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heparan sulfate/product/Biacore
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    Effect of Ac-ARPCA-NH 2 on the HSPG/FGF2/FGFR1 complex and FGFR signaling. (A) HSPG-deficient, FGFR1-transfected CHO cells were added to wild-type CHO-K1 monolayers in serum-free medium with FGF2 (1.66 nM) in the presence of Ac-ARPCA-NH 2 (d) or Ac-ARP S A-NH 2 (○) peptides. After 2 hrs of incubation at 37°C, the cells bound to the monolayer were counted under an inverted microscope. Experiments were performed in triplicate and were repeated twice with similar results. (B) GM7373 cells were treated with vehicle (a), 0.17 nM FGF2 (b), or FGF2 plus Ac-ARPCA-NH 2 (c) or Ac-ARP S A-NH 2 (•) peptides (both at 1.0 μM). After 10 min., cell extracts were analysed by Western blotting using anti-phospho-ERK 1/2 and anti-ERK 2 antibodies. (C) Sensogram overlay showing the binding of FGF2 (150 nM or 50 nM, respectively) to heparin-coated ( upper panel ) or sFGFR1(IIIc)/Fc chimera-coated ( lower panel ) BIAcore sensor chips in the absence ( _____ ) or in the presence of Ac-ARPCA-NH 2 (- - -) or Ac-ARP S A-NH 2 ( …… ) peptides (both at 1.0 μM). The response (in RU) was recorded as a function of time.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Fibroblast growth factor 2-antagonist activity of a long-pentraxin 3-derived anti-angiogenic pentapeptide

    doi: 10.1111/j.1582-4934.2009.00855.x

    Figure Lengend Snippet: Effect of Ac-ARPCA-NH 2 on the HSPG/FGF2/FGFR1 complex and FGFR signaling. (A) HSPG-deficient, FGFR1-transfected CHO cells were added to wild-type CHO-K1 monolayers in serum-free medium with FGF2 (1.66 nM) in the presence of Ac-ARPCA-NH 2 (d) or Ac-ARP S A-NH 2 (○) peptides. After 2 hrs of incubation at 37°C, the cells bound to the monolayer were counted under an inverted microscope. Experiments were performed in triplicate and were repeated twice with similar results. (B) GM7373 cells were treated with vehicle (a), 0.17 nM FGF2 (b), or FGF2 plus Ac-ARPCA-NH 2 (c) or Ac-ARP S A-NH 2 (•) peptides (both at 1.0 μM). After 10 min., cell extracts were analysed by Western blotting using anti-phospho-ERK 1/2 and anti-ERK 2 antibodies. (C) Sensogram overlay showing the binding of FGF2 (150 nM or 50 nM, respectively) to heparin-coated ( upper panel ) or sFGFR1(IIIc)/Fc chimera-coated ( lower panel ) BIAcore sensor chips in the absence ( _____ ) or in the presence of Ac-ARPCA-NH 2 (- - -) or Ac-ARP S A-NH 2 ( …… ) peptides (both at 1.0 μM). The response (in RU) was recorded as a function of time.

    Article Snippet: To assess whether the inhibition of HSPG/FGF2/FGFR1 complex formation by ARPCA is due to its ability to hamper HSPG/FGF2 and/or FGF2/FGFR1 interactions, the peptide was investigated for the capacity to prevent the binding of free FGF2 to heparin or to a sFGFR1(IIIc)/Fc chimera both immobilized to BIAcore sensor chips.

    Techniques: Transfection, Incubation, Inverted Microscopy, Western Blot, Binding Assay

    Effect of Ac-ARPCA-NH 2 on the biological activity of the FGF2/FGFR system. (A) GM 7373 cells were treated with FGF2 (0.55 nM), FGF1 (1.66 nM), FGF8b (1.66 nM), VEGF (0.7 nM), EGF (0.6 nM), DAG (15 μM), TPA (8.0 nM) or 10% FCS in the absence or in the presence of Ac-ARPCA-NH 2 (66 μM). (B) CHO cells overexpressing FGFR1, FGFR2, FGFR3 or FGFR4 were treated with FGF2 (0.55 nM) in the absence or in the presence of Ac-ARPCA-NH 2 (black bars) or Ac-ARP S A-NH 2 (open bars) (both peptides at 300 μM). In both assays, cells were trypsinized and counted 24 hrs after the stimulus. Data (mean ± S.D. of triplicate observations) are expressed as percentage of cell proliferation measured in the absence of the peptide under test.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Fibroblast growth factor 2-antagonist activity of a long-pentraxin 3-derived anti-angiogenic pentapeptide

    doi: 10.1111/j.1582-4934.2009.00855.x

    Figure Lengend Snippet: Effect of Ac-ARPCA-NH 2 on the biological activity of the FGF2/FGFR system. (A) GM 7373 cells were treated with FGF2 (0.55 nM), FGF1 (1.66 nM), FGF8b (1.66 nM), VEGF (0.7 nM), EGF (0.6 nM), DAG (15 μM), TPA (8.0 nM) or 10% FCS in the absence or in the presence of Ac-ARPCA-NH 2 (66 μM). (B) CHO cells overexpressing FGFR1, FGFR2, FGFR3 or FGFR4 were treated with FGF2 (0.55 nM) in the absence or in the presence of Ac-ARPCA-NH 2 (black bars) or Ac-ARP S A-NH 2 (open bars) (both peptides at 300 μM). In both assays, cells were trypsinized and counted 24 hrs after the stimulus. Data (mean ± S.D. of triplicate observations) are expressed as percentage of cell proliferation measured in the absence of the peptide under test.

    Article Snippet: To assess whether the inhibition of HSPG/FGF2/FGFR1 complex formation by ARPCA is due to its ability to hamper HSPG/FGF2 and/or FGF2/FGFR1 interactions, the peptide was investigated for the capacity to prevent the binding of free FGF2 to heparin or to a sFGFR1(IIIc)/Fc chimera both immobilized to BIAcore sensor chips.

    Techniques: Activity Assay

    Hypothesis of interaction between Ac-ARPCA-NH 2 and FGF2. The global minimum conformation of Ac-ARPCA-NH 2 peptide (in atom type in the figure) was superimposed to the crystal structure of FGFR1 (PDB code: 1FQ9) in the β-sheet region 164–170 of the hydrophobic domain D2 of the receptor (FGFR1 residues in purple: Leu165, Ala167 and Pro169). The best superimposition was obtained between the following amino acid pairs of the peptide and receptor, respectively: methyl group of the acetyl cap with Leu165, Ala 1 with Ala167 and Ala5 with Pro169. As shown in the figure, the peptide could mimic the highly conserved b-sheet portion of the receptor interacting with FGF2 (FGF2 residues in green: Tyr24, Phe31, Tyr103, Leu140 and Met142). The hydrophobic characteristics of FGF2 in this region are represented by orange surfaces.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Fibroblast growth factor 2-antagonist activity of a long-pentraxin 3-derived anti-angiogenic pentapeptide

    doi: 10.1111/j.1582-4934.2009.00855.x

    Figure Lengend Snippet: Hypothesis of interaction between Ac-ARPCA-NH 2 and FGF2. The global minimum conformation of Ac-ARPCA-NH 2 peptide (in atom type in the figure) was superimposed to the crystal structure of FGFR1 (PDB code: 1FQ9) in the β-sheet region 164–170 of the hydrophobic domain D2 of the receptor (FGFR1 residues in purple: Leu165, Ala167 and Pro169). The best superimposition was obtained between the following amino acid pairs of the peptide and receptor, respectively: methyl group of the acetyl cap with Leu165, Ala 1 with Ala167 and Ala5 with Pro169. As shown in the figure, the peptide could mimic the highly conserved b-sheet portion of the receptor interacting with FGF2 (FGF2 residues in green: Tyr24, Phe31, Tyr103, Leu140 and Met142). The hydrophobic characteristics of FGF2 in this region are represented by orange surfaces.

    Article Snippet: To assess whether the inhibition of HSPG/FGF2/FGFR1 complex formation by ARPCA is due to its ability to hamper HSPG/FGF2 and/or FGF2/FGFR1 interactions, the peptide was investigated for the capacity to prevent the binding of free FGF2 to heparin or to a sFGFR1(IIIc)/Fc chimera both immobilized to BIAcore sensor chips.

    Techniques:

    A-B-A SPR competition analysis of binding of glycans to NHBA. Competition of: ( i) chondroitin sulfate D disaccharide (CS) with heparin (H) or heparan sulfate (HS); ( ii) H with CS or HS; (iii) HS with CS or H. Glycans were used at concentration 10x their respective K D s, and injected in the following order: glycan 1 (A) → glycan 1 and glycan 2 (B) → glycan 1 (A). PBS is used as a control.

    Journal: Scientific Reports

    Article Title: The Bexsero Neisseria meningitidis serogroup B vaccine antigen NHBA is a high-affinity chondroitin sulfate binding protein

    doi: 10.1038/s41598-018-24639-x

    Figure Lengend Snippet: A-B-A SPR competition analysis of binding of glycans to NHBA. Competition of: ( i) chondroitin sulfate D disaccharide (CS) with heparin (H) or heparan sulfate (HS); ( ii) H with CS or HS; (iii) HS with CS or H. Glycans were used at concentration 10x their respective K D s, and injected in the following order: glycan 1 (A) → glycan 1 and glycan 2 (B) → glycan 1 (A). PBS is used as a control.

    Article Snippet: Competition between chondroitin sulfate, heparan sulfate and heparin for NHBA binding To investigate the nature of binding sites on NHBA, a competition SPR assay was performed with the three main GAG substrates of NHBA using the A-B-A function of the Biacore S200 software.

    Techniques: SPR Assay, Binding Assay, Concentration Assay, Injection

    SPR analysis of NHBA interactions with heparan sulfate, heparin and heparin disaccharides. Heparin and heparan sulfate polymers display complex, non-uniform sulfation patterns. The structures provided are representative of the major repeat units. #S: Sulfation at the # carbon. NS: No-sulfation on the specified monomer.

    Journal: Scientific Reports

    Article Title: The Bexsero Neisseria meningitidis serogroup B vaccine antigen NHBA is a high-affinity chondroitin sulfate binding protein

    doi: 10.1038/s41598-018-24639-x

    Figure Lengend Snippet: SPR analysis of NHBA interactions with heparan sulfate, heparin and heparin disaccharides. Heparin and heparan sulfate polymers display complex, non-uniform sulfation patterns. The structures provided are representative of the major repeat units. #S: Sulfation at the # carbon. NS: No-sulfation on the specified monomer.

    Article Snippet: Competition between chondroitin sulfate, heparan sulfate and heparin for NHBA binding To investigate the nature of binding sites on NHBA, a competition SPR assay was performed with the three main GAG substrates of NHBA using the A-B-A function of the Biacore S200 software.

    Techniques: SPR Assay

    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

    Journal:

    Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

    doi: 10.1074/jbc.M109.002840

    Figure Lengend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

    Article Snippet: All of these data will contribute to a better understanding of the mechanism of endostatin binding to integrins and heparan sulfate at the surface of endothelial cells.

    Techniques: SPR Assay, Binding Assay, Injection

    Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

    Journal:

    Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

    doi: 10.1074/jbc.M109.002840

    Figure Lengend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

    Article Snippet: All of these data will contribute to a better understanding of the mechanism of endostatin binding to integrins and heparan sulfate at the surface of endothelial cells.

    Techniques:

    B. burgdorferi producing ErpG binds to heparin and heparan sulfate

    Journal: Cellular microbiology

    Article Title: Host cell heparan sulfate glycosaminoglycans are ligands for OspF-related proteins of the Lyme disease spirochete

    doi: 10.1111/cmi.12448

    Figure Lengend Snippet: B. burgdorferi producing ErpG binds to heparin and heparan sulfate

    Article Snippet: Interactions of Erp proteins with heparan sulfate were analyzed by a SPR technique using a Biacore 3000 (GE Healthcare, Piscataway, NJ).

    Techniques:

    OspF-related proteins bind to heparan sulfate

    Journal: Cellular microbiology

    Article Title: Host cell heparan sulfate glycosaminoglycans are ligands for OspF-related proteins of the Lyme disease spirochete

    doi: 10.1111/cmi.12448

    Figure Lengend Snippet: OspF-related proteins bind to heparan sulfate

    Article Snippet: Interactions of Erp proteins with heparan sulfate were analyzed by a SPR technique using a Biacore 3000 (GE Healthcare, Piscataway, NJ).

    Techniques:

    Binding of ErpG-producing B. burgdorferi strain B314 to C6 glial cells is mediated by heparan sulfate

    Journal: Cellular microbiology

    Article Title: Host cell heparan sulfate glycosaminoglycans are ligands for OspF-related proteins of the Lyme disease spirochete

    doi: 10.1111/cmi.12448

    Figure Lengend Snippet: Binding of ErpG-producing B. burgdorferi strain B314 to C6 glial cells is mediated by heparan sulfate

    Article Snippet: Interactions of Erp proteins with heparan sulfate were analyzed by a SPR technique using a Biacore 3000 (GE Healthcare, Piscataway, NJ).

    Techniques: Binding Assay

    Recombinant ErpG binds to heparin and heparan sulfate

    Journal: Cellular microbiology

    Article Title: Host cell heparan sulfate glycosaminoglycans are ligands for OspF-related proteins of the Lyme disease spirochete

    doi: 10.1111/cmi.12448

    Figure Lengend Snippet: Recombinant ErpG binds to heparin and heparan sulfate

    Article Snippet: Interactions of Erp proteins with heparan sulfate were analyzed by a SPR technique using a Biacore 3000 (GE Healthcare, Piscataway, NJ).

    Techniques: Recombinant

    Recombinant ErpG proteins bind to heparan sulfate

    Journal: Cellular microbiology

    Article Title: Host cell heparan sulfate glycosaminoglycans are ligands for OspF-related proteins of the Lyme disease spirochete

    doi: 10.1111/cmi.12448

    Figure Lengend Snippet: Recombinant ErpG proteins bind to heparan sulfate

    Article Snippet: Interactions of Erp proteins with heparan sulfate were analyzed by a SPR technique using a Biacore 3000 (GE Healthcare, Piscataway, NJ).

    Techniques: Recombinant