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Figure Lengend Snippet: In vivo biodistribution and uPAR targeted accumulation of eEVs functionalized PLGA-PEG-antimiRNA nanococktail in syngeneic 4T1 subcutaneous tumors in nude mice (nu/nu). (a) In vivo fluorescence imaging showed the whole-body biodistribution and 4T1-tumor specific accumulation of ICG labeled Sc-uPA and uPA nanococktail formulations administered via tail vein injection on Days 0, 6 and 12, and imaged on Days 2, 7 and 15 using a Lago (Spectral Imaging system) in vivo imaging system. (b) Photoacoustic imaging of 4T1 tumors for the accumulation of ICG labeled eEV-uPA-PLGA-PEG-antimiRNAs on Day 16. (c) Ex vivo fluorescence imaging showed the uPAR mediated 4T1 tumor-specific accumulation of eEV-uPA-PLGA-PEG-antimiRNA-21 nanococktail formulations on Day 17. The uPA and Sc-uPA nanococktail formulations injected nude mice were sacrificed on Day 17, and the organs (liver, spleen, kidneys, heart, lungs, and brain) were collected for ex vivo imaging. (d) The biodistribution and tumor-specific accumulation of ICG, antimiRNA-21, and antimiRNA-10b in nude mice injected with uPA and Sc-uPA functionalized eEV-PLGA-PEG-antimiRNA-21 nanococktail formulations. Lago fluorescence imaging and TaqMan real-time qRT-PCR were used for organ-specific biodistribution based on the quantification of ICG and antimiRNAs. The endogenous expression of sno234 was used as an internal control to normalize the qRT-PCR results. We used 5 animals (n = 5) in each group while repeating the experiment twice in the study (n = 10).

Article Snippet: To further investigate the tumor accumulation of 4T1-eEVs-PNCs, we used a hemispherical Photoacoustic (PAI) imaging system (Nexus 128 scanner, Endra Inc., Ann Arbor, MI) as described previously.

Techniques: In Vivo, Fluorescence, Imaging, Labeling, Injection, In Vivo Imaging, Ex Vivo, Quantitative RT-PCR, Expressing, Control