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DSMZ helicobacter hepaticus infection
Helicobacter Hepaticus Infection, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC acttttatgtcccctgttgact 514 493 beta 2 microglobulin s atgatgctgcttacatgtctc 261
Acttttatgtcccctgttgact 514 493 Beta 2 Microglobulin S Atgatgctgcttacatgtctc 261, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DSMZ helicobacter hepaticus infection
Helicobacter Hepaticus Infection, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DSMZ helicobacter hepaticus
Helicobacter Hepaticus, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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XpressBio helicobacter hepaticus
The detection limit of the multiplex real-time PCR (mRT-PCR) assay. The detection limit of the assay was evaluated using 10-fold serially diluted plasmid DNA. Each serially diluted control DNA, ranging from 10 6 copies to 1 copy per reaction, was used to determine the detection limit of the RT-PCR assay. In the RT-PCR assay, the amplification curve of the specific probe for detecting SeV (A) , Mycoplama spp. (B) , R. pneumotropicus (C) , R. heylii (D) , <t>Helicobacter</t> spp. (E) , MNV (F) , MHV (G) , Salmonella spp. (H) , S. aureus (I) , S. moniliformis (J) , C. kutscheri (K) , and P. aeruginosa (L) is shown. The overall detection limit of this assay for each control DNA ranged from approximately 1 to 100 copy DNA per reaction. C T was plotted against the input of the quantity of each DNA (repeated 10 times). The linearity was generated by plotting the log quantity of each DNA versus the corresponding C T value, and the coefficient of determination of the linear regression was 0.993–1.0, with a slope ranging from −3.193 to −3.934. The fluorescence intensity is displayed on the Y -axis ( R 2 = reporter signal/passive reference signal). RFU, relative fluorescence unit; R 2 , fluorescence units.
Helicobacter Hepaticus, supplied by XpressBio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC accession nucleic amino species source number acid acid helicobacter hepaticus atcc 51449
The detection limit of the multiplex real-time PCR (mRT-PCR) assay. The detection limit of the assay was evaluated using 10-fold serially diluted plasmid DNA. Each serially diluted control DNA, ranging from 10 6 copies to 1 copy per reaction, was used to determine the detection limit of the RT-PCR assay. In the RT-PCR assay, the amplification curve of the specific probe for detecting SeV (A) , Mycoplama spp. (B) , R. pneumotropicus (C) , R. heylii (D) , <t>Helicobacter</t> spp. (E) , MNV (F) , MHV (G) , Salmonella spp. (H) , S. aureus (I) , S. moniliformis (J) , C. kutscheri (K) , and P. aeruginosa (L) is shown. The overall detection limit of this assay for each control DNA ranged from approximately 1 to 100 copy DNA per reaction. C T was plotted against the input of the quantity of each DNA (repeated 10 times). The linearity was generated by plotting the log quantity of each DNA versus the corresponding C T value, and the coefficient of determination of the linear regression was 0.993–1.0, with a slope ranging from −3.193 to −3.934. The fluorescence intensity is displayed on the Y -axis ( R 2 = reporter signal/passive reference signal). RFU, relative fluorescence unit; R 2 , fluorescence units.
Accession Nucleic Amino Species Source Number Acid Acid Helicobacter Hepaticus Atcc 51449, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Envigo helicobacter hepaticus suspension
The detection limit of the multiplex real-time PCR (mRT-PCR) assay. The detection limit of the assay was evaluated using 10-fold serially diluted plasmid DNA. Each serially diluted control DNA, ranging from 10 6 copies to 1 copy per reaction, was used to determine the detection limit of the RT-PCR assay. In the RT-PCR assay, the amplification curve of the specific probe for detecting SeV (A) , Mycoplama spp. (B) , R. pneumotropicus (C) , R. heylii (D) , <t>Helicobacter</t> spp. (E) , MNV (F) , MHV (G) , Salmonella spp. (H) , S. aureus (I) , S. moniliformis (J) , C. kutscheri (K) , and P. aeruginosa (L) is shown. The overall detection limit of this assay for each control DNA ranged from approximately 1 to 100 copy DNA per reaction. C T was plotted against the input of the quantity of each DNA (repeated 10 times). The linearity was generated by plotting the log quantity of each DNA versus the corresponding C T value, and the coefficient of determination of the linear regression was 0.993–1.0, with a slope ranging from −3.193 to −3.934. The fluorescence intensity is displayed on the Y -axis ( R 2 = reporter signal/passive reference signal). RFU, relative fluorescence unit; R 2 , fluorescence units.
Helicobacter Hepaticus Suspension, supplied by Envigo, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC helicobacter hepaticus atcc 51449
The detection limit of the multiplex real-time PCR (mRT-PCR) assay. The detection limit of the assay was evaluated using 10-fold serially diluted plasmid DNA. Each serially diluted control DNA, ranging from 10 6 copies to 1 copy per reaction, was used to determine the detection limit of the RT-PCR assay. In the RT-PCR assay, the amplification curve of the specific probe for detecting SeV (A) , Mycoplama spp. (B) , R. pneumotropicus (C) , R. heylii (D) , <t>Helicobacter</t> spp. (E) , MNV (F) , MHV (G) , Salmonella spp. (H) , S. aureus (I) , S. moniliformis (J) , C. kutscheri (K) , and P. aeruginosa (L) is shown. The overall detection limit of this assay for each control DNA ranged from approximately 1 to 100 copy DNA per reaction. C T was plotted against the input of the quantity of each DNA (repeated 10 times). The linearity was generated by plotting the log quantity of each DNA versus the corresponding C T value, and the coefficient of determination of the linear regression was 0.993–1.0, with a slope ranging from −3.193 to −3.934. The fluorescence intensity is displayed on the Y -axis ( R 2 = reporter signal/passive reference signal). RFU, relative fluorescence unit; R 2 , fluorescence units.
Helicobacter Hepaticus Atcc 51449, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/helicobacter hepaticus atcc 51449/product/ATCC
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ATCC hypothetical protein
( A ) Organization of genes within operon containing flgV (red) in H. pylori 26695. The smaller arrows upstream of aroQ and within flhG indicate identified transcriptional start sites in H. pylori 26695 . Flagellar genes in the operon are flhF (dark blue), flhG (purple), fliA (green), fliM (dark gold), and fliY (black). Other genes are aroQ (gold), folK (light blue), and three genes of unknown function (HP1036, yellow; HP1029, dark olive; and HP1028, navy blue). ( B ) Synteny of flhFGflgV in representative genera of Campylobacterota. Homologous genes are color coded as indicated in panel A. Genes absent from the H. pylori 26695 flhFGflgV operon include pepQ (light green), potential flgJ homolog (dark red), potential flgN homolog (dark orange), <t>hypothetical</t> protein (white), fliE (light blue), flgB (pink), and fliK (peach). In A. butzleri , flhFGflgV appears to be part of a larger operon of ∼30 flagellar and chemotaxis genes. ( C ) Tertiary structures of FlgV proteins from H. pylori , C. jejuni , and A. butzleri predicted by the AlphaFold2 tool in ChimeraX . The N-terminus (N) and C-terminus (C) of the protein are indicated. Regions in blue indicate a high confidence for predicted structure, while regions in yellow indicate a lower confidence for predicted structure.
Hypothetical Protein, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The detection limit of the multiplex real-time PCR (mRT-PCR) assay. The detection limit of the assay was evaluated using 10-fold serially diluted plasmid DNA. Each serially diluted control DNA, ranging from 10 6 copies to 1 copy per reaction, was used to determine the detection limit of the RT-PCR assay. In the RT-PCR assay, the amplification curve of the specific probe for detecting SeV (A) , Mycoplama spp. (B) , R. pneumotropicus (C) , R. heylii (D) , Helicobacter spp. (E) , MNV (F) , MHV (G) , Salmonella spp. (H) , S. aureus (I) , S. moniliformis (J) , C. kutscheri (K) , and P. aeruginosa (L) is shown. The overall detection limit of this assay for each control DNA ranged from approximately 1 to 100 copy DNA per reaction. C T was plotted against the input of the quantity of each DNA (repeated 10 times). The linearity was generated by plotting the log quantity of each DNA versus the corresponding C T value, and the coefficient of determination of the linear regression was 0.993–1.0, with a slope ranging from −3.193 to −3.934. The fluorescence intensity is displayed on the Y -axis ( R 2 = reporter signal/passive reference signal). RFU, relative fluorescence unit; R 2 , fluorescence units.

Journal: Frontiers in Veterinary Science

Article Title: Performance of three multiplex real-time PCR assays for simultaneous detection of 12 infectious pathogens in mice affected with respiratory and digestive diseases

doi: 10.3389/fvets.2024.1421427

Figure Lengend Snippet: The detection limit of the multiplex real-time PCR (mRT-PCR) assay. The detection limit of the assay was evaluated using 10-fold serially diluted plasmid DNA. Each serially diluted control DNA, ranging from 10 6 copies to 1 copy per reaction, was used to determine the detection limit of the RT-PCR assay. In the RT-PCR assay, the amplification curve of the specific probe for detecting SeV (A) , Mycoplama spp. (B) , R. pneumotropicus (C) , R. heylii (D) , Helicobacter spp. (E) , MNV (F) , MHV (G) , Salmonella spp. (H) , S. aureus (I) , S. moniliformis (J) , C. kutscheri (K) , and P. aeruginosa (L) is shown. The overall detection limit of this assay for each control DNA ranged from approximately 1 to 100 copy DNA per reaction. C T was plotted against the input of the quantity of each DNA (repeated 10 times). The linearity was generated by plotting the log quantity of each DNA versus the corresponding C T value, and the coefficient of determination of the linear regression was 0.993–1.0, with a slope ranging from −3.193 to −3.934. The fluorescence intensity is displayed on the Y -axis ( R 2 = reporter signal/passive reference signal). RFU, relative fluorescence unit; R 2 , fluorescence units.

Article Snippet: 5 , Helicobacter hepaticus , Xpressbio , Tissue , N/A , N/A , N/A , N/A , 25.28 , N/A , N/A , N/A , N/A , N/A , N/A , N/A.

Techniques: Multiplex Assay, Real-time Polymerase Chain Reaction, Plasmid Preparation, Control, Reverse Transcription Polymerase Chain Reaction, Amplification, Generated, Fluorescence

Analytical specificity of the mRT-PCR assay with 42 strains.

Journal: Frontiers in Veterinary Science

Article Title: Performance of three multiplex real-time PCR assays for simultaneous detection of 12 infectious pathogens in mice affected with respiratory and digestive diseases

doi: 10.3389/fvets.2024.1421427

Figure Lengend Snippet: Analytical specificity of the mRT-PCR assay with 42 strains.

Article Snippet: 5 , Helicobacter hepaticus , Xpressbio , Tissue , N/A , N/A , N/A , N/A , 25.28 , N/A , N/A , N/A , N/A , N/A , N/A , N/A.

Techniques: Virus

Results of single and multiple infections in 102 clinical samples.

Journal: Frontiers in Veterinary Science

Article Title: Performance of three multiplex real-time PCR assays for simultaneous detection of 12 infectious pathogens in mice affected with respiratory and digestive diseases

doi: 10.3389/fvets.2024.1421427

Figure Lengend Snippet: Results of single and multiple infections in 102 clinical samples.

Article Snippet: 5 , Helicobacter hepaticus , Xpressbio , Tissue , N/A , N/A , N/A , N/A , 25.28 , N/A , N/A , N/A , N/A , N/A , N/A , N/A.

Techniques: Infection

( A ) Organization of genes within operon containing flgV (red) in H. pylori 26695. The smaller arrows upstream of aroQ and within flhG indicate identified transcriptional start sites in H. pylori 26695 . Flagellar genes in the operon are flhF (dark blue), flhG (purple), fliA (green), fliM (dark gold), and fliY (black). Other genes are aroQ (gold), folK (light blue), and three genes of unknown function (HP1036, yellow; HP1029, dark olive; and HP1028, navy blue). ( B ) Synteny of flhFGflgV in representative genera of Campylobacterota. Homologous genes are color coded as indicated in panel A. Genes absent from the H. pylori 26695 flhFGflgV operon include pepQ (light green), potential flgJ homolog (dark red), potential flgN homolog (dark orange), hypothetical protein (white), fliE (light blue), flgB (pink), and fliK (peach). In A. butzleri , flhFGflgV appears to be part of a larger operon of ∼30 flagellar and chemotaxis genes. ( C ) Tertiary structures of FlgV proteins from H. pylori , C. jejuni , and A. butzleri predicted by the AlphaFold2 tool in ChimeraX . The N-terminus (N) and C-terminus (C) of the protein are indicated. Regions in blue indicate a high confidence for predicted structure, while regions in yellow indicate a lower confidence for predicted structure.

Journal: PLOS ONE

Article Title: FlgV forms a flagellar motor ring that is required for optimal motility of Helicobacter pylori

doi: 10.1371/journal.pone.0287514

Figure Lengend Snippet: ( A ) Organization of genes within operon containing flgV (red) in H. pylori 26695. The smaller arrows upstream of aroQ and within flhG indicate identified transcriptional start sites in H. pylori 26695 . Flagellar genes in the operon are flhF (dark blue), flhG (purple), fliA (green), fliM (dark gold), and fliY (black). Other genes are aroQ (gold), folK (light blue), and three genes of unknown function (HP1036, yellow; HP1029, dark olive; and HP1028, navy blue). ( B ) Synteny of flhFGflgV in representative genera of Campylobacterota. Homologous genes are color coded as indicated in panel A. Genes absent from the H. pylori 26695 flhFGflgV operon include pepQ (light green), potential flgJ homolog (dark red), potential flgN homolog (dark orange), hypothetical protein (white), fliE (light blue), flgB (pink), and fliK (peach). In A. butzleri , flhFGflgV appears to be part of a larger operon of ∼30 flagellar and chemotaxis genes. ( C ) Tertiary structures of FlgV proteins from H. pylori , C. jejuni , and A. butzleri predicted by the AlphaFold2 tool in ChimeraX . The N-terminus (N) and C-terminus (C) of the protein are indicated. Regions in blue indicate a high confidence for predicted structure, while regions in yellow indicate a lower confidence for predicted structure.

Article Snippet: hypothetical protein [ Helicobacter hepaticus ATCC 51449] , 43% , 43.14% , 118 , 1e-09 , WP_011115985.1.

Techniques: Chemotaxis Assay

C. jejuni FlgV homologs in various genera of Campylobacterota identified by blastp analysis.

Journal: PLOS ONE

Article Title: FlgV forms a flagellar motor ring that is required for optimal motility of Helicobacter pylori

doi: 10.1371/journal.pone.0287514

Figure Lengend Snippet: C. jejuni FlgV homologs in various genera of Campylobacterota identified by blastp analysis.

Article Snippet: hypothetical protein [ Helicobacter hepaticus ATCC 51449] , 43% , 43.14% , 118 , 1e-09 , WP_011115985.1.

Techniques: