hek293 gnti  (ATCC)


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    ATCC hek293 gnti
    Hek293 Gnti, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hek293s gnti strain  (ATCC)


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    ATCC hek293s gnti strain
    Hek293s Gnti Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hek293s gnti  (ATCC)


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    ATCC hek293s gnti
    Hek293s Gnti, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hek293s gnti  (ATCC)


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    ATCC hek293s gnti
    ( A ) Western blot (detecting the myc-tag attached to the C-termini of respective constructs) of CALHM subunits expressed upon co-transfection of <t>HEK293S</t> GnTI − cells with pairs of CALHM subunits. CALHM channels were isolated by affinity purification of one subunit containing a fusion to Venus and an SBP-tag (bait, shown at higher molecular weight). The resulting samples contain a mix of homomers of the purified subunit and heteromers (with the second subunit not containing Venus and SBP tags, prey at lower molecular weight). The ratio of pray/bait is displayed based on the integration of the intensity of the displayed Western blots. The experiment has been carried out once. Molecular weights are indicated. ( B–E ) Representative patch-clamp electrophysiology recordings (whole-cell configuration) of indicated CALHM subunits expressed in HEK-293 cells measured in buffers containing 2 mM Ca 2+ on the extracellular side. ( B ) Comparison of currents from mock-transfected cells (left) and cells expressing CALHM1 (right). The inset shows the voltage protocol. ( C ) Current response from cells co-transfected with DNA coding for CALHM1 and CALHM3 subunits. ( D ) Current response from cells transfected with CALHM2 (left) or CALHM4 (right) subunits. ( E ) Current response from cells co-transfected with CALHM2 and CALHM4 subunits. ( F ) Mean current density of recordings of different CALHM constructs (measured at 100 mV, 400 ms after the voltage step), errors are SEM for n>2 and differences from the mean in case of n=2, values from individual recordings are shown as circles. Figure 1—source data 1. Uncropped images of immunoblots and raw data plotted in . Figure 1—source data 2. Raw recordings displayed and plotted in .
    Hek293s Gnti, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek293s gnti/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hek293s gnti - by Bioz Stars, 2024-07
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    Images

    1) Product Images from "Structural features of heteromeric channels composed of CALHM2 and CALHM4 paralogs"

    Article Title: Structural features of heteromeric channels composed of CALHM2 and CALHM4 paralogs

    Journal: eLife

    doi: 10.7554/eLife.96138

    ( A ) Western blot (detecting the myc-tag attached to the C-termini of respective constructs) of CALHM subunits expressed upon co-transfection of HEK293S GnTI − cells with pairs of CALHM subunits. CALHM channels were isolated by affinity purification of one subunit containing a fusion to Venus and an SBP-tag (bait, shown at higher molecular weight). The resulting samples contain a mix of homomers of the purified subunit and heteromers (with the second subunit not containing Venus and SBP tags, prey at lower molecular weight). The ratio of pray/bait is displayed based on the integration of the intensity of the displayed Western blots. The experiment has been carried out once. Molecular weights are indicated. ( B–E ) Representative patch-clamp electrophysiology recordings (whole-cell configuration) of indicated CALHM subunits expressed in HEK-293 cells measured in buffers containing 2 mM Ca 2+ on the extracellular side. ( B ) Comparison of currents from mock-transfected cells (left) and cells expressing CALHM1 (right). The inset shows the voltage protocol. ( C ) Current response from cells co-transfected with DNA coding for CALHM1 and CALHM3 subunits. ( D ) Current response from cells transfected with CALHM2 (left) or CALHM4 (right) subunits. ( E ) Current response from cells co-transfected with CALHM2 and CALHM4 subunits. ( F ) Mean current density of recordings of different CALHM constructs (measured at 100 mV, 400 ms after the voltage step), errors are SEM for n>2 and differences from the mean in case of n=2, values from individual recordings are shown as circles. Figure 1—source data 1. Uncropped images of immunoblots and raw data plotted in . Figure 1—source data 2. Raw recordings displayed and plotted in .
    Figure Legend Snippet: ( A ) Western blot (detecting the myc-tag attached to the C-termini of respective constructs) of CALHM subunits expressed upon co-transfection of HEK293S GnTI − cells with pairs of CALHM subunits. CALHM channels were isolated by affinity purification of one subunit containing a fusion to Venus and an SBP-tag (bait, shown at higher molecular weight). The resulting samples contain a mix of homomers of the purified subunit and heteromers (with the second subunit not containing Venus and SBP tags, prey at lower molecular weight). The ratio of pray/bait is displayed based on the integration of the intensity of the displayed Western blots. The experiment has been carried out once. Molecular weights are indicated. ( B–E ) Representative patch-clamp electrophysiology recordings (whole-cell configuration) of indicated CALHM subunits expressed in HEK-293 cells measured in buffers containing 2 mM Ca 2+ on the extracellular side. ( B ) Comparison of currents from mock-transfected cells (left) and cells expressing CALHM1 (right). The inset shows the voltage protocol. ( C ) Current response from cells co-transfected with DNA coding for CALHM1 and CALHM3 subunits. ( D ) Current response from cells transfected with CALHM2 (left) or CALHM4 (right) subunits. ( E ) Current response from cells co-transfected with CALHM2 and CALHM4 subunits. ( F ) Mean current density of recordings of different CALHM constructs (measured at 100 mV, 400 ms after the voltage step), errors are SEM for n>2 and differences from the mean in case of n=2, values from individual recordings are shown as circles. Figure 1—source data 1. Uncropped images of immunoblots and raw data plotted in . Figure 1—source data 2. Raw recordings displayed and plotted in .

    Techniques Used: Western Blot, Construct, Cotransfection, Isolation, Affinity Purification, Molecular Weight, Purification, Patch Clamp, Comparison, Transfection, Expressing


    Figure Legend Snippet:

    Techniques Used: Recombinant, Modification, Expressing, Plasmid Preparation, Clone Assay, Western Blot, Software, Sample Prep, Protein Purification, Purification, Selection, Suspension, Binding Assay

    hek293s gnti  (ATCC)


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    ATCC hek293s gnti
    ( A ) Western blot (detecting the myc-tag attached to the C-termini of respective constructs) of CALHM subunits expressed upon co-transfection of <t>HEK293S</t> GnTI − cells with pairs of CALHM subunits. CALHM channels were isolated by affinity purification of one subunit containing a fusion to Venus and an SBP-tag (bait, shown at higher molecular weight). The resulting samples contain a mix of homomers of the purified subunit and heteromers (with the second subunit not containing Venus and SBP tags, prey at lower molecular weight). The ratio of pray/bait is displayed based on the integration of the intensity of the displayed Western blots. The experiment has been carried out once. Molecular weights are indicated. ( B–E ) Representative patch-clamp electrophysiology recordings (whole-cell configuration) of indicated CALHM subunits expressed in HEK-293 cells measured in buffers containing 2 mM Ca 2+ on the extracellular side. ( B ) Comparison of currents from mock-transfected cells (left) and cells expressing CALHM1 (right). The inset shows the voltage protocol. ( C ) Current response from cells co-transfected with DNA coding for CALHM1 and CALHM3 subunits. ( D ) Current response from cells transfected with CALHM2 (left) or CALHM4 (right) subunits. ( E ) Current response from cells co-transfected with CALHM2 and CALHM4 subunits. ( F ) Mean current density of recordings of different CALHM constructs (measured at 100 mV, 400 ms after the voltage step), errors are SEM for n>2 and differences from the mean in case of n=2, values from individual recordings are shown as circles. Figure 1—source data 1. Uncropped images of immunoblots and raw data plotted in . Figure 1—source data 2. Raw recordings displayed and plotted in .
    Hek293s Gnti, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek293s gnti/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hek293s gnti - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Structural features of heteromeric channels composed of CALHM2 and CALHM4 paralogs"

    Article Title: Structural features of heteromeric channels composed of CALHM2 and CALHM4 paralogs

    Journal: eLife

    doi: 10.7554/eLife.96138

    ( A ) Western blot (detecting the myc-tag attached to the C-termini of respective constructs) of CALHM subunits expressed upon co-transfection of HEK293S GnTI − cells with pairs of CALHM subunits. CALHM channels were isolated by affinity purification of one subunit containing a fusion to Venus and an SBP-tag (bait, shown at higher molecular weight). The resulting samples contain a mix of homomers of the purified subunit and heteromers (with the second subunit not containing Venus and SBP tags, prey at lower molecular weight). The ratio of pray/bait is displayed based on the integration of the intensity of the displayed Western blots. The experiment has been carried out once. Molecular weights are indicated. ( B–E ) Representative patch-clamp electrophysiology recordings (whole-cell configuration) of indicated CALHM subunits expressed in HEK-293 cells measured in buffers containing 2 mM Ca 2+ on the extracellular side. ( B ) Comparison of currents from mock-transfected cells (left) and cells expressing CALHM1 (right). The inset shows the voltage protocol. ( C ) Current response from cells co-transfected with DNA coding for CALHM1 and CALHM3 subunits. ( D ) Current response from cells transfected with CALHM2 (left) or CALHM4 (right) subunits. ( E ) Current response from cells co-transfected with CALHM2 and CALHM4 subunits. ( F ) Mean current density of recordings of different CALHM constructs (measured at 100 mV, 400 ms after the voltage step), errors are SEM for n>2 and differences from the mean in case of n=2, values from individual recordings are shown as circles. Figure 1—source data 1. Uncropped images of immunoblots and raw data plotted in . Figure 1—source data 2. Raw recordings displayed and plotted in .
    Figure Legend Snippet: ( A ) Western blot (detecting the myc-tag attached to the C-termini of respective constructs) of CALHM subunits expressed upon co-transfection of HEK293S GnTI − cells with pairs of CALHM subunits. CALHM channels were isolated by affinity purification of one subunit containing a fusion to Venus and an SBP-tag (bait, shown at higher molecular weight). The resulting samples contain a mix of homomers of the purified subunit and heteromers (with the second subunit not containing Venus and SBP tags, prey at lower molecular weight). The ratio of pray/bait is displayed based on the integration of the intensity of the displayed Western blots. The experiment has been carried out once. Molecular weights are indicated. ( B–E ) Representative patch-clamp electrophysiology recordings (whole-cell configuration) of indicated CALHM subunits expressed in HEK-293 cells measured in buffers containing 2 mM Ca 2+ on the extracellular side. ( B ) Comparison of currents from mock-transfected cells (left) and cells expressing CALHM1 (right). The inset shows the voltage protocol. ( C ) Current response from cells co-transfected with DNA coding for CALHM1 and CALHM3 subunits. ( D ) Current response from cells transfected with CALHM2 (left) or CALHM4 (right) subunits. ( E ) Current response from cells co-transfected with CALHM2 and CALHM4 subunits. ( F ) Mean current density of recordings of different CALHM constructs (measured at 100 mV, 400 ms after the voltage step), errors are SEM for n>2 and differences from the mean in case of n=2, values from individual recordings are shown as circles. Figure 1—source data 1. Uncropped images of immunoblots and raw data plotted in . Figure 1—source data 2. Raw recordings displayed and plotted in .

    Techniques Used: Western Blot, Construct, Cotransfection, Isolation, Affinity Purification, Molecular Weight, Purification, Patch Clamp, Comparison, Transfection, Expressing


    Figure Legend Snippet:

    Techniques Used: Recombinant, Modification, Expressing, Plasmid Preparation, Clone Assay, Western Blot, Software, Sample Prep, Protein Purification, Purification, Selection, Suspension, Binding Assay

    hek293s gnti cells  (ATCC)


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    ATCC hek293s gnti cells
    Hek293s Gnti Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hek293s gnti  (ATCC)


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    ATCC hek293s gnti
    Hek293s Gnti, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    c hek293s gnti suspension cells  (ATCC)


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    ATCC c hek293s gnti suspension cells
    C Hek293s Gnti Suspension Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hek293s gnti  (ATCC)


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    ATCC hek293s gnti
    Hek293s Gnti, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cell lines sf9 atcc crl 1711 hek293s gnti atcc crl  (ATCC)


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    ATCC cell lines sf9 atcc crl 1711 hek293s gnti atcc crl
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    ATCC hek293 gnti
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