hek293 tet on cells  (TaKaRa)


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    TaKaRa hek293 tet on cells
    Purification, biochemical and biophysical characterization of HTT-HAP40 complexes. (A) SEC elution profiles of HTT-HAP40 complexes differing in HTT polyQ length. The HTT-HAP40 complexes were purified from <t>HEK293-based</t> human cells co-expressing FLAG-tagged HTT with 17Q (17QHTT), 46Q (46QHTT) or 128Q (128QHTT), respectively, together with Strep-tagged HAP40. Cleared cell lysates were incubated with Strep-Tactin beads, washed and bound proteins were eluted with desthiobiotin prior to loading on a Superose 6 10/300 increase column. (B) Coomassie staining of the main peak of the different HTT-HAP40 eluates shown in (A). (C) Western Blot analyses of HTT-HAP40 eluates after affinity purification and SEC, using antibodies detecting HTT (upper two rows), polyQ (middle) and HAP40 (lower two rows). (D, E) Thermal unfolding of the different HTT-HAP40 complexes. Melting curves and melting points were obtained by differential scanning fluorimetry (DSF) (D) and nano differential scanning fluorimetry (nanoDSF) (E). Melting temperatures are indicated. One of three measurements is shown.
    Hek293 Tet On Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek293 tet on cells/product/TaKaRa
    Average 93 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    hek293 tet on cells - by Bioz Stars, 2022-12
    93/100 stars

    Images

    1) Product Images from "PolyQ expansion does not alter the Huntingtin-HAP40 complex"

    Article Title: PolyQ expansion does not alter the Huntingtin-HAP40 complex

    Journal: bioRxiv

    doi: 10.1101/2021.02.02.429316

    Purification, biochemical and biophysical characterization of HTT-HAP40 complexes. (A) SEC elution profiles of HTT-HAP40 complexes differing in HTT polyQ length. The HTT-HAP40 complexes were purified from HEK293-based human cells co-expressing FLAG-tagged HTT with 17Q (17QHTT), 46Q (46QHTT) or 128Q (128QHTT), respectively, together with Strep-tagged HAP40. Cleared cell lysates were incubated with Strep-Tactin beads, washed and bound proteins were eluted with desthiobiotin prior to loading on a Superose 6 10/300 increase column. (B) Coomassie staining of the main peak of the different HTT-HAP40 eluates shown in (A). (C) Western Blot analyses of HTT-HAP40 eluates after affinity purification and SEC, using antibodies detecting HTT (upper two rows), polyQ (middle) and HAP40 (lower two rows). (D, E) Thermal unfolding of the different HTT-HAP40 complexes. Melting curves and melting points were obtained by differential scanning fluorimetry (DSF) (D) and nano differential scanning fluorimetry (nanoDSF) (E). Melting temperatures are indicated. One of three measurements is shown.
    Figure Legend Snippet: Purification, biochemical and biophysical characterization of HTT-HAP40 complexes. (A) SEC elution profiles of HTT-HAP40 complexes differing in HTT polyQ length. The HTT-HAP40 complexes were purified from HEK293-based human cells co-expressing FLAG-tagged HTT with 17Q (17QHTT), 46Q (46QHTT) or 128Q (128QHTT), respectively, together with Strep-tagged HAP40. Cleared cell lysates were incubated with Strep-Tactin beads, washed and bound proteins were eluted with desthiobiotin prior to loading on a Superose 6 10/300 increase column. (B) Coomassie staining of the main peak of the different HTT-HAP40 eluates shown in (A). (C) Western Blot analyses of HTT-HAP40 eluates after affinity purification and SEC, using antibodies detecting HTT (upper two rows), polyQ (middle) and HAP40 (lower two rows). (D, E) Thermal unfolding of the different HTT-HAP40 complexes. Melting curves and melting points were obtained by differential scanning fluorimetry (DSF) (D) and nano differential scanning fluorimetry (nanoDSF) (E). Melting temperatures are indicated. One of three measurements is shown.

    Techniques Used: Purification, Expressing, Incubation, Staining, Western Blot, Affinity Purification, Nano Differential Scanning Fluorimetry

    2) Product Images from "Pharmacogenetic Aspects of the Interaction of AT1 Receptor Antagonists With ATP-Binding Cassette Transporter ABCG2"

    Article Title: Pharmacogenetic Aspects of the Interaction of AT1 Receptor Antagonists With ATP-Binding Cassette Transporter ABCG2

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2018.00463

    (A) Representative flow cytometric plots of cell surface-related ABCG2 fluorescence intensity of non-permeabilized, either untreated (non-induced) or doxycycline-treated (1 μg/mL; 24 h) HEK293-Tet-On cells transiently transfected with ABCG2 WT or ABCG2 variants, respectively. (B) Flow cytometric analysis of plasma membrane-related ABCG2 fluorescence intensity in non-permeabilized HEK293-Tet-On cells transiently transfected with ABCG2 WT or ABCG2 variant vectors, respectively. Data are shown as mean ± SD ( n = 3–18), the mean of ABCG2 WT was arbitrarily set to 100%. ∗ P
    Figure Legend Snippet: (A) Representative flow cytometric plots of cell surface-related ABCG2 fluorescence intensity of non-permeabilized, either untreated (non-induced) or doxycycline-treated (1 μg/mL; 24 h) HEK293-Tet-On cells transiently transfected with ABCG2 WT or ABCG2 variants, respectively. (B) Flow cytometric analysis of plasma membrane-related ABCG2 fluorescence intensity in non-permeabilized HEK293-Tet-On cells transiently transfected with ABCG2 WT or ABCG2 variant vectors, respectively. Data are shown as mean ± SD ( n = 3–18), the mean of ABCG2 WT was arbitrarily set to 100%. ∗ P

    Techniques Used: Flow Cytometry, Fluorescence, Transfection, Variant Assay

    (A) Analysis of ABCG2 mRNA expression in transiently transfected HEK293-Tet-On cells. Data are shown as mean ± SD ( n = 3–6), the mean of ABCG2 wild-type (WT) was arbitrarily set to 100%. ∗∗ P
    Figure Legend Snippet: (A) Analysis of ABCG2 mRNA expression in transiently transfected HEK293-Tet-On cells. Data are shown as mean ± SD ( n = 3–6), the mean of ABCG2 wild-type (WT) was arbitrarily set to 100%. ∗∗ P

    Techniques Used: Expressing, Transfection

    PhA efflux assay: concentration-dependent inhibition of ABCG2-mediated PhA efflux by telmisartan (A,B) , candesartan cilexetil (C,D) , and irbesartan (E,F) in HEK293-Tet-On cells transiently transfected with ABCG2 WT or ABCG2 variants (Y469A, M483F, and Y570A), respectively. Data are shown as mean ± SD ( n = 4–14). ∗ P
    Figure Legend Snippet: PhA efflux assay: concentration-dependent inhibition of ABCG2-mediated PhA efflux by telmisartan (A,B) , candesartan cilexetil (C,D) , and irbesartan (E,F) in HEK293-Tet-On cells transiently transfected with ABCG2 WT or ABCG2 variants (Y469A, M483F, and Y570A), respectively. Data are shown as mean ± SD ( n = 4–14). ∗ P

    Techniques Used: Concentration Assay, Inhibition, Transfection

    PhA efflux assay: concentration-dependent inhibition of ABCG2-mediated PhA efflux by telmisartan (A,B) , candesartan cilexetil (C,D) , losartan (E,F) , and irbesartan (G,H) in HEK293-Tet-On cells transiently transfected with ABCG2 WT or ABCG2 variants (F489L and R482G), respectively. Data are shown as mean ± SD ( n = 4–16). ∗ P
    Figure Legend Snippet: PhA efflux assay: concentration-dependent inhibition of ABCG2-mediated PhA efflux by telmisartan (A,B) , candesartan cilexetil (C,D) , losartan (E,F) , and irbesartan (G,H) in HEK293-Tet-On cells transiently transfected with ABCG2 WT or ABCG2 variants (F489L and R482G), respectively. Data are shown as mean ± SD ( n = 4–16). ∗ P

    Techniques Used: Concentration Assay, Inhibition, Transfection

    3) Product Images from "Continuous, real-time bioimaging of chemical bioavailability and toxicology using autonomously bioluminescent human cell lines"

    Article Title: Continuous, real-time bioimaging of chemical bioavailability and toxicology using autonomously bioluminescent human cell lines

    Journal: Proceedings of SPIE--the International Society for Optical Engineering

    doi: 10.1117/12.2015030

    Continuous real-time monitoring of the bioluminescent response to doxycycline treatment of the HEK293 Tet- lux reporter cells.
    Figure Legend Snippet: Continuous real-time monitoring of the bioluminescent response to doxycycline treatment of the HEK293 Tet- lux reporter cells.

    Techniques Used:

    4) Product Images from "Differential Roles of Hypoxia-Inducible Factor 1? (HIF-1?) and HIF-2? in Hypoxic Gene Regulation"

    Article Title: Differential Roles of Hypoxia-Inducible Factor 1? (HIF-1?) and HIF-2? in Hypoxic Gene Regulation

    Journal: Molecular and Cellular Biology

    doi: 10.1128/MCB.23.24.9361-9374.2003

    Establishment of HEK293 TET-on HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.
    Figure Legend Snippet: Establishment of HEK293 TET-on HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.

    Techniques Used: Expressing, Northern Blot, Concentration Assay

    5) Product Images from "Differential Roles of Hypoxia-Inducible Factor 1? (HIF-1?) and HIF-2? in Hypoxic Gene Regulation"

    Article Title: Differential Roles of Hypoxia-Inducible Factor 1? (HIF-1?) and HIF-2? in Hypoxic Gene Regulation

    Journal: Molecular and Cellular Biology

    doi: 10.1128/MCB.23.24.9361-9374.2003

    Establishment of HEK293 TET-on HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.
    Figure Legend Snippet: Establishment of HEK293 TET-on HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.

    Techniques Used: Expressing, Northern Blot, Concentration Assay

    6) Product Images from "Differential Roles of Hypoxia-Inducible Factor 1? (HIF-1?) and HIF-2? in Hypoxic Gene Regulation"

    Article Title: Differential Roles of Hypoxia-Inducible Factor 1? (HIF-1?) and HIF-2? in Hypoxic Gene Regulation

    Journal: Molecular and Cellular Biology

    doi: 10.1128/MCB.23.24.9361-9374.2003

    Establishment of HEK293 TET-on HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.
    Figure Legend Snippet: Establishment of HEK293 TET-on HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.

    Techniques Used: Expressing, Northern Blot, Concentration Assay

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    TaKaRa hek293t cells
    Hek293t Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek293t cells/product/TaKaRa
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    hek293t cells - by Bioz Stars, 2022-12
    95/100 stars
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