hek293 tet on cells  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    TaKaRa hek293 tet on cells
    Establishment of <t>HEK293</t> <t>TET-on</t> HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.
    Hek293 Tet On Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek293 tet on cells/product/TaKaRa
    Average 93 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    hek293 tet on cells - by Bioz Stars, 2022-08
    93/100 stars

    Images

    1) Product Images from "Differential Roles of Hypoxia-Inducible Factor 1? (HIF-1?) and HIF-2? in Hypoxic Gene Regulation"

    Article Title: Differential Roles of Hypoxia-Inducible Factor 1? (HIF-1?) and HIF-2? in Hypoxic Gene Regulation

    Journal: Molecular and Cellular Biology

    doi: 10.1128/MCB.23.24.9361-9374.2003

    Establishment of HEK293 TET-on HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.
    Figure Legend Snippet: Establishment of HEK293 TET-on HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.

    Techniques Used: Expressing, Northern Blot, Concentration Assay

    2) Product Images from "Differential Roles of Hypoxia-Inducible Factor 1? (HIF-1?) and HIF-2? in Hypoxic Gene Regulation"

    Article Title: Differential Roles of Hypoxia-Inducible Factor 1? (HIF-1?) and HIF-2? in Hypoxic Gene Regulation

    Journal: Molecular and Cellular Biology

    doi: 10.1128/MCB.23.24.9361-9374.2003

    Establishment of HEK293 TET-on HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.
    Figure Legend Snippet: Establishment of HEK293 TET-on HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.

    Techniques Used: Expressing, Northern Blot, Concentration Assay

    3) Product Images from "Pharmacogenetic Aspects of the Interaction of AT1 Receptor Antagonists With ATP-Binding Cassette Transporter ABCG2"

    Article Title: Pharmacogenetic Aspects of the Interaction of AT1 Receptor Antagonists With ATP-Binding Cassette Transporter ABCG2

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2018.00463

    (A) Representative flow cytometric plots of cell surface-related ABCG2 fluorescence intensity of non-permeabilized, either untreated (non-induced) or doxycycline-treated (1 μg/mL; 24 h) HEK293-Tet-On cells transiently transfected with ABCG2 WT or ABCG2 variants, respectively. (B) Flow cytometric analysis of plasma membrane-related ABCG2 fluorescence intensity in non-permeabilized HEK293-Tet-On cells transiently transfected with ABCG2 WT or ABCG2 variant vectors, respectively. Data are shown as mean ± SD ( n = 3–18), the mean of ABCG2 WT was arbitrarily set to 100%. ∗ P
    Figure Legend Snippet: (A) Representative flow cytometric plots of cell surface-related ABCG2 fluorescence intensity of non-permeabilized, either untreated (non-induced) or doxycycline-treated (1 μg/mL; 24 h) HEK293-Tet-On cells transiently transfected with ABCG2 WT or ABCG2 variants, respectively. (B) Flow cytometric analysis of plasma membrane-related ABCG2 fluorescence intensity in non-permeabilized HEK293-Tet-On cells transiently transfected with ABCG2 WT or ABCG2 variant vectors, respectively. Data are shown as mean ± SD ( n = 3–18), the mean of ABCG2 WT was arbitrarily set to 100%. ∗ P

    Techniques Used: Flow Cytometry, Fluorescence, Transfection, Variant Assay

    (A) Analysis of ABCG2 mRNA expression in transiently transfected HEK293-Tet-On cells. Data are shown as mean ± SD ( n = 3–6), the mean of ABCG2 wild-type (WT) was arbitrarily set to 100%. ∗∗ P
    Figure Legend Snippet: (A) Analysis of ABCG2 mRNA expression in transiently transfected HEK293-Tet-On cells. Data are shown as mean ± SD ( n = 3–6), the mean of ABCG2 wild-type (WT) was arbitrarily set to 100%. ∗∗ P

    Techniques Used: Expressing, Transfection

    PhA efflux assay: concentration-dependent inhibition of ABCG2-mediated PhA efflux by telmisartan (A,B) , candesartan cilexetil (C,D) , and irbesartan (E,F) in HEK293-Tet-On cells transiently transfected with ABCG2 WT or ABCG2 variants (Y469A, M483F, and Y570A), respectively. Data are shown as mean ± SD ( n = 4–14). ∗ P
    Figure Legend Snippet: PhA efflux assay: concentration-dependent inhibition of ABCG2-mediated PhA efflux by telmisartan (A,B) , candesartan cilexetil (C,D) , and irbesartan (E,F) in HEK293-Tet-On cells transiently transfected with ABCG2 WT or ABCG2 variants (Y469A, M483F, and Y570A), respectively. Data are shown as mean ± SD ( n = 4–14). ∗ P

    Techniques Used: Concentration Assay, Inhibition, Transfection

    PhA efflux assay: concentration-dependent inhibition of ABCG2-mediated PhA efflux by telmisartan (A,B) , candesartan cilexetil (C,D) , losartan (E,F) , and irbesartan (G,H) in HEK293-Tet-On cells transiently transfected with ABCG2 WT or ABCG2 variants (F489L and R482G), respectively. Data are shown as mean ± SD ( n = 4–16). ∗ P
    Figure Legend Snippet: PhA efflux assay: concentration-dependent inhibition of ABCG2-mediated PhA efflux by telmisartan (A,B) , candesartan cilexetil (C,D) , losartan (E,F) , and irbesartan (G,H) in HEK293-Tet-On cells transiently transfected with ABCG2 WT or ABCG2 variants (F489L and R482G), respectively. Data are shown as mean ± SD ( n = 4–16). ∗ P

    Techniques Used: Concentration Assay, Inhibition, Transfection

    4) Product Images from "Continuous, real-time bioimaging of chemical bioavailability and toxicology using autonomously bioluminescent human cell lines"

    Article Title: Continuous, real-time bioimaging of chemical bioavailability and toxicology using autonomously bioluminescent human cell lines

    Journal: Proceedings of SPIE--the International Society for Optical Engineering

    doi: 10.1117/12.2015030

    Continuous real-time monitoring of the bioluminescent response to doxycycline treatment of the HEK293 Tet- lux reporter cells.
    Figure Legend Snippet: Continuous real-time monitoring of the bioluminescent response to doxycycline treatment of the HEK293 Tet- lux reporter cells.

    Techniques Used:

    5) Product Images from "PolyQ expansion does not alter the Huntingtin-HAP40 complex"

    Article Title: PolyQ expansion does not alter the Huntingtin-HAP40 complex

    Journal: bioRxiv

    doi: 10.1101/2021.02.02.429316

    Purification, biochemical and biophysical characterization of HTT-HAP40 complexes. (A) SEC elution profiles of HTT-HAP40 complexes differing in HTT polyQ length. The HTT-HAP40 complexes were purified from HEK293-based human cells co-expressing FLAG-tagged HTT with 17Q (17QHTT), 46Q (46QHTT) or 128Q (128QHTT), respectively, together with Strep-tagged HAP40. Cleared cell lysates were incubated with Strep-Tactin beads, washed and bound proteins were eluted with desthiobiotin prior to loading on a Superose 6 10/300 increase column. (B) Coomassie staining of the main peak of the different HTT-HAP40 eluates shown in (A). (C) Western Blot analyses of HTT-HAP40 eluates after affinity purification and SEC, using antibodies detecting HTT (upper two rows), polyQ (middle) and HAP40 (lower two rows). (D, E) Thermal unfolding of the different HTT-HAP40 complexes. Melting curves and melting points were obtained by differential scanning fluorimetry (DSF) (D) and nano differential scanning fluorimetry (nanoDSF) (E). Melting temperatures are indicated. One of three measurements is shown.
    Figure Legend Snippet: Purification, biochemical and biophysical characterization of HTT-HAP40 complexes. (A) SEC elution profiles of HTT-HAP40 complexes differing in HTT polyQ length. The HTT-HAP40 complexes were purified from HEK293-based human cells co-expressing FLAG-tagged HTT with 17Q (17QHTT), 46Q (46QHTT) or 128Q (128QHTT), respectively, together with Strep-tagged HAP40. Cleared cell lysates were incubated with Strep-Tactin beads, washed and bound proteins were eluted with desthiobiotin prior to loading on a Superose 6 10/300 increase column. (B) Coomassie staining of the main peak of the different HTT-HAP40 eluates shown in (A). (C) Western Blot analyses of HTT-HAP40 eluates after affinity purification and SEC, using antibodies detecting HTT (upper two rows), polyQ (middle) and HAP40 (lower two rows). (D, E) Thermal unfolding of the different HTT-HAP40 complexes. Melting curves and melting points were obtained by differential scanning fluorimetry (DSF) (D) and nano differential scanning fluorimetry (nanoDSF) (E). Melting temperatures are indicated. One of three measurements is shown.

    Techniques Used: Purification, Expressing, Incubation, Staining, Western Blot, Affinity Purification, Nano Differential Scanning Fluorimetry

    6) Product Images from "Differential Roles of Hypoxia-Inducible Factor 1? (HIF-1?) and HIF-2? in Hypoxic Gene Regulation"

    Article Title: Differential Roles of Hypoxia-Inducible Factor 1? (HIF-1?) and HIF-2? in Hypoxic Gene Regulation

    Journal: Molecular and Cellular Biology

    doi: 10.1128/MCB.23.24.9361-9374.2003

    Establishment of HEK293 TET-on HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.
    Figure Legend Snippet: Establishment of HEK293 TET-on HIF-1αDPA and HIF-2αDPA clones. (A) HIF-α mRNA expression is tightly regulated by doxycycline. Northern blot analysis of HIF-1α and HIF-2α mRNA expression in HEK293 TET-on HIF-1αDPA clone 130 (left) and HEK293 TET-on HIF-2αDPA clone 63 (right) treated with different amounts of doxycycline for 20 h. (B) Doxycycline (1 μg/ml for 20 h) induced HIF-1α mRNA and protein expression in two independent HEK293 TET-on HIF-1αDPA clones (left) and HIF-2α mRNA and protein expression in two independent HEK293 TET-on HIF-2αDPA clones (right). Dox. conc., concentration of doxycycline.

    Techniques Used: Expressing, Northern Blot, Concentration Assay

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    TaKaRa hek293 cells
    Rescue effect of SNX2 proteins on influenza A/DkPen PA inhibitory activity to host gene expression. The <t>HEK293</t> cells were grown in a 24-well plate (5 × 10 4 cells/well) for 24 h in standard culture conditions and transfected with constant amounts of pCAGGS-PA(D) (1 ng/well) and pSEAP (20 ng/well) plasmids and indicated amounts of the SNX2 plasmids in the figure. After 24 h of transfection, the reporter SEAP activities in culture media was detected with a commercial kit
    Hek293 Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek293 cells/product/TaKaRa
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hek293 cells - by Bioz Stars, 2022-08
    97/100 stars
      Buy from Supplier

    Image Search Results


    Rescue effect of SNX2 proteins on influenza A/DkPen PA inhibitory activity to host gene expression. The HEK293 cells were grown in a 24-well plate (5 × 10 4 cells/well) for 24 h in standard culture conditions and transfected with constant amounts of pCAGGS-PA(D) (1 ng/well) and pSEAP (20 ng/well) plasmids and indicated amounts of the SNX2 plasmids in the figure. After 24 h of transfection, the reporter SEAP activities in culture media was detected with a commercial kit

    Journal: Molecular Biology Reports

    Article Title: Human sorting nexin 2 protein interacts with Influenza A virus PA protein and has a negative regulatory effect on the virus replication

    doi: 10.1007/s11033-021-06906-9

    Figure Lengend Snippet: Rescue effect of SNX2 proteins on influenza A/DkPen PA inhibitory activity to host gene expression. The HEK293 cells were grown in a 24-well plate (5 × 10 4 cells/well) for 24 h in standard culture conditions and transfected with constant amounts of pCAGGS-PA(D) (1 ng/well) and pSEAP (20 ng/well) plasmids and indicated amounts of the SNX2 plasmids in the figure. After 24 h of transfection, the reporter SEAP activities in culture media was detected with a commercial kit

    Article Snippet: The proteins were found to be efficiently synthesized in transfected HEK293 cells (data not shown).

    Techniques: Activity Assay, Expressing, Transfection

    Characteristic cellular localization of GCAP1-GFP co-expressed with RetGC1. A , fluorescence of GCAP1-GFP in HEK293 cells expressed in the absence ( a ) and presence ( b-d ) of RetGC1, nc , nuclei; ni, nucleoli; a and b, fluorescence of GCAP1-GFP; c , immunofluorescence

    Journal:

    Article Title: Binding of Guanylyl Cyclase Activating Protein 1 (GCAP1) to Retinal Guanylyl Cyclase (RetGC1)

    doi: 10.1074/jbc.M801899200

    Figure Lengend Snippet: Characteristic cellular localization of GCAP1-GFP co-expressed with RetGC1. A , fluorescence of GCAP1-GFP in HEK293 cells expressed in the absence ( a ) and presence ( b-d ) of RetGC1, nc , nuclei; ni, nucleoli; a and b, fluorescence of GCAP1-GFP; c , immunofluorescence

    Article Snippet: Co-localization of the E75Q/E111Q/E155Q mutant with RetGC1 in HEK293 cells was consistent with the ability of the Mg2+ -liganded GCAP1, the physiological form of GCAP1 in light-adapted photoreceptor cells , to activate the cyclase ( ).

    Techniques: Fluorescence, Immunofluorescence

    Properties of dsRed-RetGC1 and GCAP1-GFP. A , activation of non-tagged RetGC1 in HEK293 cell membranes by purified non-tagged (□) or GFP-tagged (○) GCAP1; B , Ca 2+ sensitivity of the non-tagged RetGC1 reconstituted with the non-tagged

    Journal:

    Article Title: Binding of Guanylyl Cyclase Activating Protein 1 (GCAP1) to Retinal Guanylyl Cyclase (RetGC1)

    doi: 10.1074/jbc.M801899200

    Figure Lengend Snippet: Properties of dsRed-RetGC1 and GCAP1-GFP. A , activation of non-tagged RetGC1 in HEK293 cell membranes by purified non-tagged (□) or GFP-tagged (○) GCAP1; B , Ca 2+ sensitivity of the non-tagged RetGC1 reconstituted with the non-tagged

    Article Snippet: Co-localization of the E75Q/E111Q/E155Q mutant with RetGC1 in HEK293 cells was consistent with the ability of the Mg2+ -liganded GCAP1, the physiological form of GCAP1 in light-adapted photoreceptor cells , to activate the cyclase ( ).

    Techniques: Activation Assay, Purification

    The effects of influenza A virus PA proteins on the expression of SEAP reporter in transiently transfected HEK293 cells. A. The cells expressing native PA proteins. B. The cells expressing chimeric PA proteins. C. The cells expressing influenza PA proteins with WSN PB2 and PB1. D. The cells expressing influenza PA proteins with DkPen, PB2 and PB1. Total plasmid DNA adjusted to 250 ng/well with pCAGGS plasmid DNA (Niwa et al., 1991).

    Journal: Turkish Journal of Biology

    Article Title: Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase

    doi: 10.3906/biy-2005-73

    Figure Lengend Snippet: The effects of influenza A virus PA proteins on the expression of SEAP reporter in transiently transfected HEK293 cells. A. The cells expressing native PA proteins. B. The cells expressing chimeric PA proteins. C. The cells expressing influenza PA proteins with WSN PB2 and PB1. D. The cells expressing influenza PA proteins with DkPen, PB2 and PB1. Total plasmid DNA adjusted to 250 ng/well with pCAGGS plasmid DNA (Niwa et al., 1991).

    Article Snippet: In contrast to viral RdRP complex, more aggressive changes at the gene transcript level were observed in the cells expressing the PA subunit alone compared to control HEK293 cells.

    Techniques: Expressing, Transfection, Plasmid Preparation

    The heatmaps and Venn diagram of the genes related to the interferon response in HEK293 cells infected with influenza A viruses (WSN or DkPen) and noninfected cells (HEK293).

    Journal: Turkish Journal of Biology

    Article Title: Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase

    doi: 10.3906/biy-2005-73

    Figure Lengend Snippet: The heatmaps and Venn diagram of the genes related to the interferon response in HEK293 cells infected with influenza A viruses (WSN or DkPen) and noninfected cells (HEK293).

    Article Snippet: In contrast to viral RdRP complex, more aggressive changes at the gene transcript level were observed in the cells expressing the PA subunit alone compared to control HEK293 cells.

    Techniques: Infection

    The heatmaps and Venn diagram of the interferon response genes in HEK293 cells transiently transected with plasmid DNAs expressing influenza A virus RdRP enzyme (3P).

    Journal: Turkish Journal of Biology

    Article Title: Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase

    doi: 10.3906/biy-2005-73

    Figure Lengend Snippet: The heatmaps and Venn diagram of the interferon response genes in HEK293 cells transiently transected with plasmid DNAs expressing influenza A virus RdRP enzyme (3P).

    Article Snippet: In contrast to viral RdRP complex, more aggressive changes at the gene transcript level were observed in the cells expressing the PA subunit alone compared to control HEK293 cells.

    Techniques: Plasmid Preparation, Expressing

    The heatmaps and Venn diagram of the interferon response genes in HEK293 cells transiently transected with plasmid DNAs expressing the PA subunit of influenza A virus RdRP.

    Journal: Turkish Journal of Biology

    Article Title: Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase

    doi: 10.3906/biy-2005-73

    Figure Lengend Snippet: The heatmaps and Venn diagram of the interferon response genes in HEK293 cells transiently transected with plasmid DNAs expressing the PA subunit of influenza A virus RdRP.

    Article Snippet: In contrast to viral RdRP complex, more aggressive changes at the gene transcript level were observed in the cells expressing the PA subunit alone compared to control HEK293 cells.

    Techniques: Plasmid Preparation, Expressing

    Western blot analysis of native actin beta and HA-actin proteins in transiently transfected HEK293 cells. The cells were cotransfected with pCHA-ACTB plasmid and a plasmid expressing deleted PA protein (pCAGGS-cPA/DkPen, pCAGGS-cPA/WSN, pCAGGS-nPA/DkPen or pCAGGS-nPA/WSN). Actin beta and viral PA proteins were separated on 10% polyacrylamide gel and immunoblotted with monoclonal mouse anti-HA (for HA-ACTB), monoclonal anti actin (for ACTB and HA-ACTB) and rabbit polyclonal anti-PA antibodies.

    Journal: Turkish Journal of Biology

    Article Title: Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase

    doi: 10.3906/biy-2005-73

    Figure Lengend Snippet: Western blot analysis of native actin beta and HA-actin proteins in transiently transfected HEK293 cells. The cells were cotransfected with pCHA-ACTB plasmid and a plasmid expressing deleted PA protein (pCAGGS-cPA/DkPen, pCAGGS-cPA/WSN, pCAGGS-nPA/DkPen or pCAGGS-nPA/WSN). Actin beta and viral PA proteins were separated on 10% polyacrylamide gel and immunoblotted with monoclonal mouse anti-HA (for HA-ACTB), monoclonal anti actin (for ACTB and HA-ACTB) and rabbit polyclonal anti-PA antibodies.

    Article Snippet: In contrast to viral RdRP complex, more aggressive changes at the gene transcript level were observed in the cells expressing the PA subunit alone compared to control HEK293 cells.

    Techniques: Western Blot, Transfection, Plasmid Preparation, Expressing

    The expression profiles of interferon response genes in HEK293 cells infected with influenza A viruses (WSN or DkPen) and noninfected cells (HEK293).

    Journal: Turkish Journal of Biology

    Article Title: Expression profiles of interferon-related genes in cells infected with influenza A viruses or transiently transfected with plasmids encoding viral RNA polymerase

    doi: 10.3906/biy-2005-73

    Figure Lengend Snippet: The expression profiles of interferon response genes in HEK293 cells infected with influenza A viruses (WSN or DkPen) and noninfected cells (HEK293).

    Article Snippet: In contrast to viral RdRP complex, more aggressive changes at the gene transcript level were observed in the cells expressing the PA subunit alone compared to control HEK293 cells.

    Techniques: Expressing, Infection