Journal: Virulence

Article Title: Of mice and men: Interaction of Corynebacterium diphtheriae strains with murine and human phagocytes

doi: 10.1080/21505594.2019.1614384

Figure Lengend Snippet: Bacterial strains and cell lines used in this study.

Article Snippet: HEK-Blue 293 hTLR9 , human TLR9/NF-κB/SEAP reporter HEK293 cells , InvivoGen, San Diego, USA.

Techniques: Isolation, Construct

Journal: Virulence

Article Title: Of mice and men: Interaction of Corynebacterium diphtheriae strains with murine and human phagocytes

doi: 10.1080/21505594.2019.1614384

Figure Lengend Snippet: Bacterial strains and cell lines used in this study.

Article Snippet: HEK-Blue 293 hTLR9 , human TLR9/NF-κB/SEAP reporter HEK293 cells , InvivoGen, San Diego, USA.

Techniques: Isolation, Construct

Journal: International Journal of Inflammation

Article Title: NPT1220-312, a TLR2/TLR9 Small Molecule Antagonist, Inhibits Pro-Inflammatory Signaling, Cytokine Release, and NLRP3 Inflammasome Activation

doi: 10.1155/2022/2337363

Figure Lengend Snippet: NPT1220-312 selectively inhibits TLR2 and TLR9. NPT1220-312 inhibition of (a) TLR1/2 as activated by Pam 3 CSK 4 and TLR2/6 as activated by Pam 2 CSK 4 in HEK293 cells overexpressing hTLR2; (b) TLR3 as activated by Poly I:C in HEK293 cells overexpressing hTLR3; (c) TLR4 as activated by LPS in HEK293 cells overexpressing hTLR4; (d) TLR7 as activated by R848 and as activated by CL-264 in HEK293 cells overexpressing hTLR7; (e) TLR8 as activated by R848 in HEK293 cells overexpressing hTLR8; and (f) TLR9 as activated by ODN2006 in HEK293 cells overexpressing hTLR9. TNF α was incorporated as a control for non-specific inhibition of signaling in each assay.

Article Snippet: HEK-Blue hTLR9 SEAP reporter 293 cells, expressing the human TLR9 gene; RAW-Blue cells derived from RAW 264.7 macrophages, stably expressing the NF- κ B and AP-1-inducible SEAP reporter gene; and THP1-XBlue cells that stably express NF- κ B and the AP-1-inducible SEAP reporter gene were obtained from InvivoGen.

Techniques: Inhibition

Journal: International Journal of Molecular Sciences

Article Title: Newly Isolated Animal Pathogen Corynebacterium silvaticum Is Cytotoxic to Human Epithelial Cells

doi: 10.3390/ijms22073549

Figure Lengend Snippet: Colonization of epithelial cells. Adhesion of the toxigenic C. ulcerans strain KL756 and the non-toxigenic C. silvaticum isolate W25 to different human epithelial cell lines: ( a ) Detroit 562 cells, ( b ) HEK-Blue 293 hTLR2 cells, ( c ) HeLa cells. The respective cell line was seeded 24 h prior to infection and infected with bacteria at a multiplicity of infection (MOI) of 50 for 90 min. Columns and error bars represent the results and standard deviations of three independent biological replicates carried out with three technical replicates each ( n = 9). Adhesion efficiency was calculated based on the ratio of colony-forming units (CFU) prior to infection and CFU on the lysate plates after infection, multiplied by 100.

Article Snippet: HEK-Blue 293 hTLR9 , Human TLR9/NFкB/SEAP reporter HEK293 cells , Invivogen.

Techniques: Infection

Journal: International Journal of Molecular Sciences

Article Title: Newly Isolated Animal Pathogen Corynebacterium silvaticum Is Cytotoxic to Human Epithelial Cells

doi: 10.3390/ijms22073549

Figure Lengend Snippet: TLR2 receptor activation by C. glutamicum ATCC13032, C. ulcerans KL756 and C. silvaticum W25 . HEK-Blue 293 hTLR2 receptor cells were infected at a MOI of 100 (white), 10 (light grey) and 1 (dark grey) with viable (*) and UV-killed (†) bacteria. Heat-killed Listeria monocytogenes (HKLM) (blue) were used as positive and phosphate-buffered saline (PBS) (light blue) as negative control. Experiments were performed in three independent biological replicates each in technical triplicates and mean values and standard deviations are shown.

Article Snippet: HEK-Blue 293 hTLR9 , Human TLR9/NFкB/SEAP reporter HEK293 cells , Invivogen.

Techniques: Activation Assay, Infection, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: Newly Isolated Animal Pathogen Corynebacterium silvaticum Is Cytotoxic to Human Epithelial Cells

doi: 10.3390/ijms22073549

Figure Lengend Snippet: HEK 293 hTLR2 reporter cell assay. Cells were infected with viable (*) and UV-killed (†) bacteria at MOI 100, MOI 10 and MOI 1 for 16 h. Heat-killed L. monocytogenes (HKLM) served as positive and PBS as negative control.

Article Snippet: HEK-Blue 293 hTLR9 , Human TLR9/NFкB/SEAP reporter HEK293 cells , Invivogen.

Techniques: Infection, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: Newly Isolated Animal Pathogen Corynebacterium silvaticum Is Cytotoxic to Human Epithelial Cells

doi: 10.3390/ijms22073549

Figure Lengend Snippet: Bacterial strains, plasmids and cell lines used in this study.

Article Snippet: HEK-Blue 293 hTLR9 , Human TLR9/NFкB/SEAP reporter HEK293 cells , Invivogen.

Techniques: