heated 60°c cell lysis solution  (Qiagen)

 
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    Name:
    Cell Lysis Solution
    Description:
    For purification of archive quality DNA from a wide range of sample types Kit contents 125 ml Cell Lysis Solution Benefits Archive quality DNA for long term storage Reproducible DNA purification Convenient scalable purification proced
    Catalog Number:
    158906
    Price:
    87
    Category:
    Puregene Accessories
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    Structured Review

    Qiagen heated 60°c cell lysis solution
    Cell Lysis Solution
    For purification of archive quality DNA from a wide range of sample types Kit contents 125 ml Cell Lysis Solution Benefits Archive quality DNA for long term storage Reproducible DNA purification Convenient scalable purification proced
    https://www.bioz.com/result/heated 60°c cell lysis solution/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    heated 60°c cell lysis solution - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Sample Preservation, DNA or RNA Extraction and Data Analysis for High-Throughput Phytoplankton Community Sequencing
    Article Snippet: .. Cellular DNA was extracted from frozen and Lugol preserved cells using DNeasy Plant Mini Kit (Qiagen, United States), Power Water DNA Isolation Kit (MoBio Laboratories, Inc., Carlsbad, CA, United States), and Power Biofilm DNA Isolation Kit (MoBio Laboratories, Inc., Carlsbad, CA, United States). .. To determine, if addition of mechanical cell destruction would improve the cell lysis and consequently DNA yield, DNeasy Plant Mini Kit DNA + mech. extraction was done using manufacturer’s instruction with additional mechanical treatments.

    Transfection:

    Article Title: Nuclear Factor I transcription factors regulate IGF binding protein 5 gene transcription in human osteoblasts
    Article Snippet: .. TE85 and MG63 cells were transiently transfected at 50% confluency in 6 well plates with 0.5 μg of IGFBP5 promoter-Luc plasmid DNA and 0.125 to 1μg of each murine Nfi expression vector, using SuperFect (Qiagen) for TE85 cells or TurboFectin 8.0 (OriGene Technologies) for MG63 cells. .. Total plasmid DNA was adjusted by addition of pCH.

    Article Title: In Vitro and in Vivo Anti-tumor Activity of miR-221/222 Inhibitors in Multiple Myeloma
    Article Snippet: .. 24 hours after transfection cells were collected and used for total RNA (tRNA) extraction by Trizol lysis buffer and column purification with RNeasy kit (Qiagen, Hilden, Germany). .. A total of 300 ng RNA were used as starting material for preparing the hybridization target by using the Ambion® WT Expression Kit (Ambion, Life Techologies).

    Article Title: In Vivo Activity of MiR-34a Mimics Delivered by Stable Nucleic Acid Lipid Particles (SNALPs) against Multiple Myeloma
    Article Snippet: .. 24 hours after transfection cells were collected and used for total RNA (tRNA) extraction by Trizol lysis buffer and column purification with RNeasy kit (Qiagen, Hilden, Germany). .. A total of 300 ng RNA were used as starting material for preparing the hybridization target by using the Ambion® WT Expression Kit (Ambion, Life Techologies).

    Magnetic Beads:

    Article Title: High-throughput genotyping of a full voltage-gated sodium channel gene via genomic DNA using target capture sequencing and analytical pipeline MoNaS to discover novel insecticide resistance mutations
    Article Snippet: .. The whole body of a single insect was homogenized in a PCR well containing 50 μl of the Lysis & Binding Solution and zirconia beads (ø 2 mm; Nikkato) in TissueLyser II (QIAGEN) at 25 Hz for 30 s. After that, the samples were centrifuged at 2000 × g for 1 min to precipitate large debris, and each supernatant was transferred to a new well containing 50 μl of the Lysis & Binding Solution and 5 μl of DNA-binding Magnetic Beads. ..

    Purification:

    Article Title: In Vitro and in Vivo Anti-tumor Activity of miR-221/222 Inhibitors in Multiple Myeloma
    Article Snippet: .. 24 hours after transfection cells were collected and used for total RNA (tRNA) extraction by Trizol lysis buffer and column purification with RNeasy kit (Qiagen, Hilden, Germany). .. A total of 300 ng RNA were used as starting material for preparing the hybridization target by using the Ambion® WT Expression Kit (Ambion, Life Techologies).

    Article Title: In Vivo Activity of MiR-34a Mimics Delivered by Stable Nucleic Acid Lipid Particles (SNALPs) against Multiple Myeloma
    Article Snippet: .. 24 hours after transfection cells were collected and used for total RNA (tRNA) extraction by Trizol lysis buffer and column purification with RNeasy kit (Qiagen, Hilden, Germany). .. A total of 300 ng RNA were used as starting material for preparing the hybridization target by using the Ambion® WT Expression Kit (Ambion, Life Techologies).

    Expressing:

    Article Title: Nuclear Factor I transcription factors regulate IGF binding protein 5 gene transcription in human osteoblasts
    Article Snippet: .. TE85 and MG63 cells were transiently transfected at 50% confluency in 6 well plates with 0.5 μg of IGFBP5 promoter-Luc plasmid DNA and 0.125 to 1μg of each murine Nfi expression vector, using SuperFect (Qiagen) for TE85 cells or TurboFectin 8.0 (OriGene Technologies) for MG63 cells. .. Total plasmid DNA was adjusted by addition of pCH.

    Polymerase Chain Reaction:

    Article Title: High-throughput genotyping of a full voltage-gated sodium channel gene via genomic DNA using target capture sequencing and analytical pipeline MoNaS to discover novel insecticide resistance mutations
    Article Snippet: .. The whole body of a single insect was homogenized in a PCR well containing 50 μl of the Lysis & Binding Solution and zirconia beads (ø 2 mm; Nikkato) in TissueLyser II (QIAGEN) at 25 Hz for 30 s. After that, the samples were centrifuged at 2000 × g for 1 min to precipitate large debris, and each supernatant was transferred to a new well containing 50 μl of the Lysis & Binding Solution and 5 μl of DNA-binding Magnetic Beads. ..

    Lysis:

    Article Title: Gene Catchr--Gene Cloning And Tagging for Caenorhabditis elegans using yeast Homologous Recombination: a novel approach for the analysis of gene expression
    Article Snippet: .. Yeast plasmid rescue Plasmid DNA was prepared from yeast by either of two methods: (i) cell wall lysis in zymolyase (ICN)followed by standard miniprep (Qiagen, Inc.) or (ii) phenol:chloroform extraction coupled with glass beads (0.5 mm, BioSpec Products, Inc.) as described previously ( ) with minor modifications: after vortexing the yeast cells in the presence of lysis buffer and glass beads, 200 µl of TE, pH 8.0, was added, the samples were spun for 5 min and the aqueous phase collected. .. NaOAc (3M) (30 µl) and 1 ml of 100% ethanol were added to the aqueous phase, the samples were spun for 10 min, and the supernatant removed.

    Article Title: Hepatic HMOX1 Expression Positively Correlates with Bach-1 and miR-122 in Patients with HCV Mono and HIV/HCV Coinfection
    Article Snippet: .. Briefly, frozen samples were homogenized in 300 µl of Lysis/Binding Solution using a TissueRuptor homogenizer (Qiagen). .. RNA was eluted in 100 µl RNase-free water and quantified using PicoDrop spectrophotometer.

    Article Title: In Vitro and in Vivo Anti-tumor Activity of miR-221/222 Inhibitors in Multiple Myeloma
    Article Snippet: .. 24 hours after transfection cells were collected and used for total RNA (tRNA) extraction by Trizol lysis buffer and column purification with RNeasy kit (Qiagen, Hilden, Germany). .. A total of 300 ng RNA were used as starting material for preparing the hybridization target by using the Ambion® WT Expression Kit (Ambion, Life Techologies).

    Article Title: High-throughput genotyping of a full voltage-gated sodium channel gene via genomic DNA using target capture sequencing and analytical pipeline MoNaS to discover novel insecticide resistance mutations
    Article Snippet: .. The whole body of a single insect was homogenized in a PCR well containing 50 μl of the Lysis & Binding Solution and zirconia beads (ø 2 mm; Nikkato) in TissueLyser II (QIAGEN) at 25 Hz for 30 s. After that, the samples were centrifuged at 2000 × g for 1 min to precipitate large debris, and each supernatant was transferred to a new well containing 50 μl of the Lysis & Binding Solution and 5 μl of DNA-binding Magnetic Beads. ..

    Article Title: In Vivo Activity of MiR-34a Mimics Delivered by Stable Nucleic Acid Lipid Particles (SNALPs) against Multiple Myeloma
    Article Snippet: .. 24 hours after transfection cells were collected and used for total RNA (tRNA) extraction by Trizol lysis buffer and column purification with RNeasy kit (Qiagen, Hilden, Germany). .. A total of 300 ng RNA were used as starting material for preparing the hybridization target by using the Ambion® WT Expression Kit (Ambion, Life Techologies).

    Binding Assay:

    Article Title: High-throughput genotyping of a full voltage-gated sodium channel gene via genomic DNA using target capture sequencing and analytical pipeline MoNaS to discover novel insecticide resistance mutations
    Article Snippet: .. The whole body of a single insect was homogenized in a PCR well containing 50 μl of the Lysis & Binding Solution and zirconia beads (ø 2 mm; Nikkato) in TissueLyser II (QIAGEN) at 25 Hz for 30 s. After that, the samples were centrifuged at 2000 × g for 1 min to precipitate large debris, and each supernatant was transferred to a new well containing 50 μl of the Lysis & Binding Solution and 5 μl of DNA-binding Magnetic Beads. ..

    Plasmid Preparation:

    Article Title: Gene Catchr--Gene Cloning And Tagging for Caenorhabditis elegans using yeast Homologous Recombination: a novel approach for the analysis of gene expression
    Article Snippet: .. Yeast plasmid rescue Plasmid DNA was prepared from yeast by either of two methods: (i) cell wall lysis in zymolyase (ICN)followed by standard miniprep (Qiagen, Inc.) or (ii) phenol:chloroform extraction coupled with glass beads (0.5 mm, BioSpec Products, Inc.) as described previously ( ) with minor modifications: after vortexing the yeast cells in the presence of lysis buffer and glass beads, 200 µl of TE, pH 8.0, was added, the samples were spun for 5 min and the aqueous phase collected. .. NaOAc (3M) (30 µl) and 1 ml of 100% ethanol were added to the aqueous phase, the samples were spun for 10 min, and the supernatant removed.

    Article Title: Nuclear Factor I transcription factors regulate IGF binding protein 5 gene transcription in human osteoblasts
    Article Snippet: .. TE85 and MG63 cells were transiently transfected at 50% confluency in 6 well plates with 0.5 μg of IGFBP5 promoter-Luc plasmid DNA and 0.125 to 1μg of each murine Nfi expression vector, using SuperFect (Qiagen) for TE85 cells or TurboFectin 8.0 (OriGene Technologies) for MG63 cells. .. Total plasmid DNA was adjusted by addition of pCH.

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  • About
  • News
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  • Team
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  • Contact
  • Bioz Stars
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  • 99
    Qiagen heated 60°c cell lysis solution
    Heated 60°C Cell Lysis Solution, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heated 60°c cell lysis solution/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    heated 60°c cell lysis solution - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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