heat stable phusion dna polymerase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher heat stable phusion dna polymerase
    Heat Stable Phusion Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heat stable phusion dna polymerase/product/Thermo Fisher
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    heat stable phusion dna polymerase - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    Construct:

    Article Title: A factor XIa-activatable hirudin-albumin fusion protein reduces thrombosis in mice without promoting blood loss
    Article Snippet: Three of four novel expression plasmids were constructed as follows. .. Plasmid pPICZ9ssHV3HSAH6 [ , ] was DNA-amplified using sense and antisense paired oligodeoxyribonucleotide primers in reactions catalysed by heat-stable Phusion DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA).

    Expressing:

    Article Title: A factor XIa-activatable hirudin-albumin fusion protein reduces thrombosis in mice without promoting blood loss
    Article Snippet: Paragraph title: Construction of expression plasmids and transformation of Pichia pastoris ... Plasmid pPICZ9ssHV3HSAH6 [ , ] was DNA-amplified using sense and antisense paired oligodeoxyribonucleotide primers in reactions catalysed by heat-stable Phusion DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA).

    Polymerase Chain Reaction:

    Article Title: A factor XIa-activatable hirudin-albumin fusion protein reduces thrombosis in mice without promoting blood loss
    Article Snippet: Plasmid pPICZ9ssHV3HSAH6 [ , ] was DNA-amplified using sense and antisense paired oligodeoxyribonucleotide primers in reactions catalysed by heat-stable Phusion DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA). .. Following PCR, products were restricted with XhoI and XbaI, gel-purified, and ligated to the 5237 bp XhoI-XbaI double digestion fragment of pPICZ9ssHV3HSAH6 .

    Transformation Assay:

    Article Title: A factor XIa-activatable hirudin-albumin fusion protein reduces thrombosis in mice without promoting blood loss
    Article Snippet: Paragraph title: Construction of expression plasmids and transformation of Pichia pastoris ... Plasmid pPICZ9ssHV3HSAH6 [ , ] was DNA-amplified using sense and antisense paired oligodeoxyribonucleotide primers in reactions catalysed by heat-stable Phusion DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA).

    Plasmid Preparation:

    Article Title: A factor XIa-activatable hirudin-albumin fusion protein reduces thrombosis in mice without promoting blood loss
    Article Snippet: .. Plasmid pPICZ9ssHV3HSAH6 [ , ] was DNA-amplified using sense and antisense paired oligodeoxyribonucleotide primers in reactions catalysed by heat-stable Phusion DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA). .. Primer sequences for each construct are given in Table .

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    Thermo Fisher high fidelity heat stable dna polymerase
    Characterization of Round 10 <t>aptamers</t> as inhibitors of FXIa-mediated amidolysis. ( A ) Means ± SEM (n = 3) of colour generation following FXIa-mediated amidolysis of chromogenic substrate S2366, in the presence (black bars) of Round 10 aptamers identified on the x axis or a scrambled negative control sequence (SCRAPT), or in the absence of added <t>DNA</t> molecules (white bar). ( B ) Mfold-generated predicted secondary structure of FELIAP. The positions of base substitutions between FELIAP and other Round 10-selected aptamers are indicated by arrows. ( C ) DNA sequence of Round 10 aptamers and SCRAPT. The sequence corresponding to the original 40 nucleotide hypervariable core of the aptamer library is shown, with base substitutions relative to FELIAP in bold.
    High Fidelity Heat Stable Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high fidelity heat stable dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high fidelity heat stable dna polymerase - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    94
    Thermo Fisher heat stable phusion dna polymerase
    Characterization of Round 10 <t>aptamers</t> as inhibitors of FXIa-mediated amidolysis. ( A ) Means ± SEM (n = 3) of colour generation following FXIa-mediated amidolysis of chromogenic substrate S2366, in the presence (black bars) of Round 10 aptamers identified on the x axis or a scrambled negative control sequence (SCRAPT), or in the absence of added <t>DNA</t> molecules (white bar). ( B ) Mfold-generated predicted secondary structure of FELIAP. The positions of base substitutions between FELIAP and other Round 10-selected aptamers are indicated by arrows. ( C ) DNA sequence of Round 10 aptamers and SCRAPT. The sequence corresponding to the original 40 nucleotide hypervariable core of the aptamer library is shown, with base substitutions relative to FELIAP in bold.
    Heat Stable Phusion Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heat stable phusion dna polymerase/product/Thermo Fisher
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    heat stable phusion dna polymerase - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    Image Search Results


    Characterization of Round 10 aptamers as inhibitors of FXIa-mediated amidolysis. ( A ) Means ± SEM (n = 3) of colour generation following FXIa-mediated amidolysis of chromogenic substrate S2366, in the presence (black bars) of Round 10 aptamers identified on the x axis or a scrambled negative control sequence (SCRAPT), or in the absence of added DNA molecules (white bar). ( B ) Mfold-generated predicted secondary structure of FELIAP. The positions of base substitutions between FELIAP and other Round 10-selected aptamers are indicated by arrows. ( C ) DNA sequence of Round 10 aptamers and SCRAPT. The sequence corresponding to the original 40 nucleotide hypervariable core of the aptamer library is shown, with base substitutions relative to FELIAP in bold.

    Journal: Scientific Reports

    Article Title: Selection and characterization of a DNA aptamer inhibiting coagulation factor XIa

    doi: 10.1038/s41598-017-02055-x

    Figure Lengend Snippet: Characterization of Round 10 aptamers as inhibitors of FXIa-mediated amidolysis. ( A ) Means ± SEM (n = 3) of colour generation following FXIa-mediated amidolysis of chromogenic substrate S2366, in the presence (black bars) of Round 10 aptamers identified on the x axis or a scrambled negative control sequence (SCRAPT), or in the absence of added DNA molecules (white bar). ( B ) Mfold-generated predicted secondary structure of FELIAP. The positions of base substitutions between FELIAP and other Round 10-selected aptamers are indicated by arrows. ( C ) DNA sequence of Round 10 aptamers and SCRAPT. The sequence corresponding to the original 40 nucleotide hypervariable core of the aptamer library is shown, with base substitutions relative to FELIAP in bold.

    Article Snippet: The pool of selected aptamers was then PCR-amplified using a high-fidelity heat-stable DNA polymerase (Phusion; Thermo Fisher Scientific) in an asymmetric PCR protocol in which 13-fold more primer A than primer B was employed; the ssDNA sense strand was then purified by preparative agarose gel electrophoresis using a 2% (w/vol) agarose gel and an Ultrafree-DA centrifugal filter unit (Millipore Sigma, Billerica, MA) to produce the amplified aptamer library and conclude the first selection round (Round 1).

    Techniques: Negative Control, Sequencing, Generated

    Characterization of truncated derivatives of FELIAP as inhibitors of FXIa-mediated amidolysis. ( A ) Extent of truncation analysis. Sequences to the left of bolded lines crossing the Mfold-generated predicted secondary structure of FELIAP are identified as FELIAP_X, where X = the length of the truncated aptamer (32, 38, 42, 49, 55, 64, or 74 for full-length FELIAP. ( B ) Tabular depiction of truncated aptamers relative to FELIAP; for instance, FELIAP_32 (relative sequence 22–53) is identical to FELIAP except for the deletion of 21 nucleotides from the 5′end and 21 nucleotides from the 3′ end of FELIAP. ( C ) Means ± SEM (n = 3) of colour generation following FXIa-mediated amidolysis of chromogenic substrate S2366, in the presence (black bars) of SCRAPT, FELIAP, or truncated derivatives of FELIAP identified in panels A and B, or in the absence of of added DNA molecules (white bar).

    Journal: Scientific Reports

    Article Title: Selection and characterization of a DNA aptamer inhibiting coagulation factor XIa

    doi: 10.1038/s41598-017-02055-x

    Figure Lengend Snippet: Characterization of truncated derivatives of FELIAP as inhibitors of FXIa-mediated amidolysis. ( A ) Extent of truncation analysis. Sequences to the left of bolded lines crossing the Mfold-generated predicted secondary structure of FELIAP are identified as FELIAP_X, where X = the length of the truncated aptamer (32, 38, 42, 49, 55, 64, or 74 for full-length FELIAP. ( B ) Tabular depiction of truncated aptamers relative to FELIAP; for instance, FELIAP_32 (relative sequence 22–53) is identical to FELIAP except for the deletion of 21 nucleotides from the 5′end and 21 nucleotides from the 3′ end of FELIAP. ( C ) Means ± SEM (n = 3) of colour generation following FXIa-mediated amidolysis of chromogenic substrate S2366, in the presence (black bars) of SCRAPT, FELIAP, or truncated derivatives of FELIAP identified in panels A and B, or in the absence of of added DNA molecules (white bar).

    Article Snippet: The pool of selected aptamers was then PCR-amplified using a high-fidelity heat-stable DNA polymerase (Phusion; Thermo Fisher Scientific) in an asymmetric PCR protocol in which 13-fold more primer A than primer B was employed; the ssDNA sense strand was then purified by preparative agarose gel electrophoresis using a 2% (w/vol) agarose gel and an Ultrafree-DA centrifugal filter unit (Millipore Sigma, Billerica, MA) to produce the amplified aptamer library and conclude the first selection round (Round 1).

    Techniques: Generated, Sequencing