heat inactivated fetal bovine serum fbs  (Thermo Fisher)


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    Name:
    Fetal Bovine Serum qualified
    Description:
    Gibco fetal bovine sera offers excellent value for basic cell culture specialty research and specific assays earning the trust of researchers with consistent quality and award winning support that helps meet your research needs and budget requirementsSera Category SecureOrigin New ZealandEndotoxin level 10 EU ml Hemoglobin level 30 mg dl levels routinely 25 mg dl
    Catalog Number:
    10091130
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cell Culture|Mammalian Cell Culture
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    Thermo Fisher heat inactivated fetal bovine serum fbs
    Gibco fetal bovine sera offers excellent value for basic cell culture specialty research and specific assays earning the trust of researchers with consistent quality and award winning support that helps meet your research needs and budget requirementsSera Category SecureOrigin New ZealandEndotoxin level 10 EU ml Hemoglobin level 30 mg dl levels routinely 25 mg dl
    https://www.bioz.com/result/heat inactivated fetal bovine serum fbs/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    heat inactivated fetal bovine serum fbs - by Bioz Stars, 2021-03
    99/100 stars

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    Cell Culture:

    Article Title: Glioma-Associated Oncogene Homolog Inhibitors Have the Potential of Suppressing Cancer Stem Cells of Breast Cancer
    Article Snippet: Cell Culture MDA-MB-231 cells were purchased from Bioresource Collection and Research Center, Taiwan. .. Cells were cultured in L15 (Hyclone, South Logan, UT, USA) containing 10% fetal bovine serum (FBS), 100 unit/mL penicillin, 100 mg/mL streptomycin and 0.1 mM NEAA (Gibco, Carlsbad, CA, USA) in a humidified atmosphere without CO2 at 37 °C. ..

    Article Title: Extracellular Caspase-8 Dependent Apoptosis on HeLa Cancer Cells and MRC-5 Normal Cells by ICD-85 (Venom Derived Peptides)
    Article Snippet: Also, is the apoptotic inducing activity of ICD-85 on cancer cells differs from normal cells? .. Materials and Methods Chemicals The Dulbecco's Modified Eagle Medium (DMEM) as cell culture medium, Fetal Bovine Serum (FBS), penicillin and streptomycin were purchased from Gibco BRL (Life Technologies, Paisley, Scotland). ..

    Modification:

    Article Title: 15-Lipoxygenase-1-mediated metabolism of docosahexaenoic acid is required for syndecan-1 signaling and apoptosis in prostate cancer cells
    Article Snippet: COX-1 small interfering RNA (siRNA) (Cat. No. SI00301511), COX-2 siRNA (Cat. No. SI00301525), 15-LOX-1-siRNA (Cat. No. SI00022449), 15-LOX-2 siRNA (Cat. No. SI00022498), 12-LOX-siRNA (Cat. No. SI00294679), 5-LOX-siRNA (Cat. No. SI00294714) and control siRNA with no known human target (Cat. No. 1027280) were purchased from Qiagen (Austin, TX). .. PC3, LNCaP and DU145 cells were purchased from the American Type Culture Collection (Manassas, VA) and maintained in advanced Dulbecco’s modified Eagle’s medium (Invitrogen, Grand Island, NY) containing 1% fetal bovine serum (FBS) (PC3 cells), RPMI 1640 medium (Invitrogen) with 10% FBS (LNCaP cells) or Eagle’s minimum essential medium (Invitrogen) containing 10% FBS (DU145 cells). ..

    Article Title: MELK is an oncogenic kinase essential for metastasis, mitotic progression, and programmed death in lung carcinoma
    Article Snippet: Emricasan, OTSSP167, and Z-DEVD-FMK were purchased from Targetmol (Shanghai, China). .. Fetal bovine serum (FBS) and Dulbecco’s Modified Eagle Medium (DMEM) were bought from Gibco (Grand Island, NY, USA). .. The goat-anti-rabbit secondary antibody, rabbit monoclonal N-cadherin, E-cadherin, Vimentin, Slug, Beta-catenin, PCNA, SPAG5, CDC25C, phospho-T48 CDC25C, phospho-S216 CDC25C, PLK1, phospho-T210 PLK1, CDK1, phospho-T161 CDK1, phospho-Y48 CDK1, caspase 3, cleaved caspase 3 (cle-caspase 3), and cleaved PARP1 (cle-PARP1) were obtained from Cell Signaling Technology (Danvers, MA,USA).

    Article Title: Pseudolaric acid B attenuates atopic dermatitis-like skin lesions by inhibiting interleukin-17-induced inflammation
    Article Snippet: The purity of PB was > 98% determined by HPLC analysis . .. Dulbecco’s modified Eagle’s medium (DMEM), RPMI-1640 medium, and fetal bovine serum (FBS) were purchased from Gibco BRL (Grand Island, NY, USA). .. PD, DMSO, DNFB (≥99% pure), monensin, ionomycin, phorbol-12-myristate-13-acetate (PMA) and GW9662 were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Article Title: Extracellular Caspase-8 Dependent Apoptosis on HeLa Cancer Cells and MRC-5 Normal Cells by ICD-85 (Venom Derived Peptides)
    Article Snippet: Also, is the apoptotic inducing activity of ICD-85 on cancer cells differs from normal cells? .. Materials and Methods Chemicals The Dulbecco's Modified Eagle Medium (DMEM) as cell culture medium, Fetal Bovine Serum (FBS), penicillin and streptomycin were purchased from Gibco BRL (Life Technologies, Paisley, Scotland). ..

    Article Title: Regulation of Villin by Wnt5a/Ror2 Signaling in Human Intestinal Cells
    Article Snippet: Fetally derived, non-transformed, human intestinal epithelial cells (HIEC) were obtained from Dr. Boudreau (University de Sherbrooke, Sherbrooke, QC, Canada). .. The HT29 and L-cells were grown in Dulbecco’s modified eagle media (DMEM) supplemented with 10% fetal bovine serum (FBS), and 0.05% Penstrep (all purchased from Invitrogen, Carlsbad, CA, USA). .. The non-transformed fetal HIECs were cultured in OPTI-MEM supplemented with 4% FBS (Invitrogen), 0.05% l -glutamine (Invitrogen), 0.02% EGF (Sigma, Oakville ON, Canada), and 0.05% HEPES (BioShop, Burlington, ON, Canada).

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    Thermo Fisher heat inactivated fetal bovine serum fbs
    TGF-β production was increased in metallothionein-injected mice. Lymph node cells obtained on day 34 post mortem were cultured in <t>RPMI</t> medium supplemented with 1·25% <t>FBS</t> in the absence (□) or presence (▪) of 250 μg/ml bovine CII for 3 days. The culture supernatants were collected and analysed for TGF-β levels using cytokine sandwich ELISA. The result of a representative experiment out of three performed is shown.
    Heat Inactivated Fetal Bovine Serum Fbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heat inactivated fetal bovine serum fbs/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    TGF-β production was increased in metallothionein-injected mice. Lymph node cells obtained on day 34 post mortem were cultured in RPMI medium supplemented with 1·25% FBS in the absence (□) or presence (▪) of 250 μg/ml bovine CII for 3 days. The culture supernatants were collected and analysed for TGF-β levels using cytokine sandwich ELISA. The result of a representative experiment out of three performed is shown.

    Journal: Clinical and Experimental Immunology

    Article Title: Metallothionein suppresses collagen-induced arthritis via induction of TGF-? and down-regulation of proinflammatory mediators

    doi: 10.1046/j.1365-2249.2002.01922.x

    Figure Lengend Snippet: TGF-β production was increased in metallothionein-injected mice. Lymph node cells obtained on day 34 post mortem were cultured in RPMI medium supplemented with 1·25% FBS in the absence (□) or presence (▪) of 250 μg/ml bovine CII for 3 days. The culture supernatants were collected and analysed for TGF-β levels using cytokine sandwich ELISA. The result of a representative experiment out of three performed is shown.

    Article Snippet: Cells were cultured in 96 well plates at a density of 1 × 106 cells/ml (200 μl/well) in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum (FBS), 2 m ml -glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, and 5 × 10−5 M 2-mercaptoethanol (Life Technologies, Rockville, MD, USA).

    Techniques: Injection, Mouse Assay, Cell Culture, Sandwich ELISA

    Roscovitine reduces glioma cell viability and potentiates the cytotoxic effects of TMZ on glioma cells in vitro . ( A ) Effect of RSV on cell viability in U87, U373 human glioma cell lines and C6, rat glioma cell line. The cells were treated with increasing concentrations of RSV for 72 h and cell viability was assessed by MTT assay. ( B ) Effect of TMZ and RSV co treatment on cell viability of U87 and C6 glioma cell lines. Cells were treated first with indicated concentrations of RSV followed by co-treatment with 100 µM TMZ. After 72 h incubation with the drugs cell viability was measured by MTT assay. ( C ) Long term colony formation assay for U87 and C6 cells after treatment with either TMZ, RSV or TMZ + RSV. The cells were treated with indicated concentrations of drug(s) for 72 h. After that medium was replaced with fresh DMEM supplemented with 10% FBS and cells were allowed to grow for 14 days with media change every third day. On 14 th day, colonies were fixed and visualized by staining with 0.05% crystal violet for 30 min. *p

    Journal: Scientific Reports

    Article Title: Roscovitine effectively enhances antitumor activity of temozolomide in vitro and in vivo mediated by increased autophagy and Caspase-3 dependent apoptosis

    doi: 10.1038/s41598-019-41380-1

    Figure Lengend Snippet: Roscovitine reduces glioma cell viability and potentiates the cytotoxic effects of TMZ on glioma cells in vitro . ( A ) Effect of RSV on cell viability in U87, U373 human glioma cell lines and C6, rat glioma cell line. The cells were treated with increasing concentrations of RSV for 72 h and cell viability was assessed by MTT assay. ( B ) Effect of TMZ and RSV co treatment on cell viability of U87 and C6 glioma cell lines. Cells were treated first with indicated concentrations of RSV followed by co-treatment with 100 µM TMZ. After 72 h incubation with the drugs cell viability was measured by MTT assay. ( C ) Long term colony formation assay for U87 and C6 cells after treatment with either TMZ, RSV or TMZ + RSV. The cells were treated with indicated concentrations of drug(s) for 72 h. After that medium was replaced with fresh DMEM supplemented with 10% FBS and cells were allowed to grow for 14 days with media change every third day. On 14 th day, colonies were fixed and visualized by staining with 0.05% crystal violet for 30 min. *p

    Article Snippet: Cells were routinely cultured in Dulbecco’s Modified Eagles Medium (DMEM) supplemented with 10% heat- inactivated fetal bovine serum (FBS) (Gibco, NY, USA), penicillin (100 U/ml) and streptomycin (100 mg/ml) (Invitrogen Life Technologies, CA, USA) and maintained at 37 °C in a 5% CO2 humidified incubator (Thermo Fisher Scientific, OH, USA).

    Techniques: In Vitro, MTT Assay, Incubation, Colony Assay, Staining

    Effects of A1254 exposure on GFAP immunoreactivity in dbcAMP differentiating C6 cells. C6 cells were subjected to different treatments, fixed and then immunocytochemistry was performed as detailed in Section 2.4 . C6 cells were cultured in DMEM with 10% FBS (A–C) or kept in serum-free DMEM in presence (G–I) or absence (D–F) of 0.1% (v/v) DMSO (vehicle). Cells exposed to: 3 μM A1254 (J–L); 9 μM A1254 (M–O); 1 mM dbcAMP (P–R). dbcAMP-differentiating cells exposed to A1254 3 μM (S–U) or 9 μM (V–X). After the treatments, cells were subjected to GFAP immunostaining (green) (A, D, G, J, M, P, S and V). DAPI-nuclear stain (blue) of the same field (B, E, H, K, N, Q, T and W). Merge for composite images (C, F, I, L, O, R, U and X). All the treatments were performed for 24 h under serum-free conditions in presence of 0.1% (v/v) DMSO used as vehicle for A1254. Scale bar = 50 μm. (For interpretation of color in Fig. 3, the reader is referred to the web version of this article.)

    Journal: FEBS Open Bio

    Article Title: Polychlorinated biphenyls impair dibutyryl cAMP-induced astrocytic differentiation in rat C6 glial cell line

    doi: 10.1016/j.fob.2013.10.008

    Figure Lengend Snippet: Effects of A1254 exposure on GFAP immunoreactivity in dbcAMP differentiating C6 cells. C6 cells were subjected to different treatments, fixed and then immunocytochemistry was performed as detailed in Section 2.4 . C6 cells were cultured in DMEM with 10% FBS (A–C) or kept in serum-free DMEM in presence (G–I) or absence (D–F) of 0.1% (v/v) DMSO (vehicle). Cells exposed to: 3 μM A1254 (J–L); 9 μM A1254 (M–O); 1 mM dbcAMP (P–R). dbcAMP-differentiating cells exposed to A1254 3 μM (S–U) or 9 μM (V–X). After the treatments, cells were subjected to GFAP immunostaining (green) (A, D, G, J, M, P, S and V). DAPI-nuclear stain (blue) of the same field (B, E, H, K, N, Q, T and W). Merge for composite images (C, F, I, L, O, R, U and X). All the treatments were performed for 24 h under serum-free conditions in presence of 0.1% (v/v) DMSO used as vehicle for A1254. Scale bar = 50 μm. (For interpretation of color in Fig. 3, the reader is referred to the web version of this article.)

    Article Snippet: 2.2 Cell cultures and treatments The C6 rat glial cell line [ ] (American Type Culture Collection, ATTC CCL-107) was cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Invitrogen, Life Technologies, Italy), 1.5 mM l -glutamine, 100 units/ml penicillin, and 100 μg/ml streptomycin under humidified atmosphere of 5% CO2 at 37 °C.

    Techniques: Immunocytochemistry, Cell Culture, Immunostaining, Staining

    Effect of EPA on EPA on PC3 ( a ) cell migration and ( b ) invasion. Double-chambered cell culture dishes with a transwell insert separating the two chambers were used to evaluate PC3 cell migration and invasion. Cells were seeded in the upper chamber, which was uncoated (migration) or coated (invasion) with Matrigel, while the lower chamber was filled with DMEM containing 10% FBS. Data represent mean + SEM ( n = 3). * P

    Journal: Lipids in Health and Disease

    Article Title: Contribution of Pyk2 pathway and reactive oxygen species (ROS) to the anti-cancer effects of eicosapentaenoic acid (EPA) in PC3 prostate cancer cells

    doi: 10.1186/s12944-019-1122-4

    Figure Lengend Snippet: Effect of EPA on EPA on PC3 ( a ) cell migration and ( b ) invasion. Double-chambered cell culture dishes with a transwell insert separating the two chambers were used to evaluate PC3 cell migration and invasion. Cells were seeded in the upper chamber, which was uncoated (migration) or coated (invasion) with Matrigel, while the lower chamber was filled with DMEM containing 10% FBS. Data represent mean + SEM ( n = 3). * P

    Article Snippet: Cell culture PC3 cells (Riken BRC Cell Bank, Tsukuba, Japan) were grown in DMEM (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/mL of penicillin, and 100 μg/mL of streptomycin (Gibco).

    Techniques: Migration, Cell Culture