heat inactivated fetal bovine serum fbs  (Thermo Fisher)

Journal: Clinical and Experimental Immunology

Article Title: Metallothionein suppresses collagen-induced arthritis via induction of TGF-? and down-regulation of proinflammatory mediators

doi: 10.1046/j.1365-2249.2002.01922.x

Figure Lengend Snippet: TGF-β production was increased in metallothionein-injected mice. Lymph node cells obtained on day 34 post mortem were cultured in RPMI medium supplemented with 1·25% FBS in the absence (□) or presence (▪) of 250 μg/ml bovine CII for 3 days. The culture supernatants were collected and analysed for TGF-β levels using cytokine sandwich ELISA. The result of a representative experiment out of three performed is shown.

Article Snippet: Cells were cultured in 96 well plates at a density of 1 × 106 cells/ml (200 μl/well) in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum (FBS), 2 m ml -glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin, and 5 × 10−5 M 2-mercaptoethanol (Life Technologies, Rockville, MD, USA).

Techniques: Injection, Mouse Assay, Cell Culture, Sandwich ELISA

Journal: Scientific Reports

Article Title: Roscovitine effectively enhances antitumor activity of temozolomide in vitro and in vivo mediated by increased autophagy and Caspase-3 dependent apoptosis

doi: 10.1038/s41598-019-41380-1

Figure Lengend Snippet: Roscovitine reduces glioma cell viability and potentiates the cytotoxic effects of TMZ on glioma cells in vitro . ( A ) Effect of RSV on cell viability in U87, U373 human glioma cell lines and C6, rat glioma cell line. The cells were treated with increasing concentrations of RSV for 72 h and cell viability was assessed by MTT assay. ( B ) Effect of TMZ and RSV co treatment on cell viability of U87 and C6 glioma cell lines. Cells were treated first with indicated concentrations of RSV followed by co-treatment with 100 µM TMZ. After 72 h incubation with the drugs cell viability was measured by MTT assay. ( C ) Long term colony formation assay for U87 and C6 cells after treatment with either TMZ, RSV or TMZ + RSV. The cells were treated with indicated concentrations of drug(s) for 72 h. After that medium was replaced with fresh DMEM supplemented with 10% FBS and cells were allowed to grow for 14 days with media change every third day. On 14 th day, colonies were fixed and visualized by staining with 0.05% crystal violet for 30 min. *p

Article Snippet: Cells were routinely cultured in Dulbecco’s Modified Eagles Medium (DMEM) supplemented with 10% heat- inactivated fetal bovine serum (FBS) (Gibco, NY, USA), penicillin (100 U/ml) and streptomycin (100 mg/ml) (Invitrogen Life Technologies, CA, USA) and maintained at 37 °C in a 5% CO2 humidified incubator (Thermo Fisher Scientific, OH, USA).

Techniques: In Vitro, MTT Assay, Incubation, Colony Assay, Staining

Journal: FEBS Open Bio

Article Title: Polychlorinated biphenyls impair dibutyryl cAMP-induced astrocytic differentiation in rat C6 glial cell line

doi: 10.1016/j.fob.2013.10.008

Figure Lengend Snippet: Effects of A1254 exposure on GFAP immunoreactivity in dbcAMP differentiating C6 cells. C6 cells were subjected to different treatments, fixed and then immunocytochemistry was performed as detailed in Section 2.4 . C6 cells were cultured in DMEM with 10% FBS (A–C) or kept in serum-free DMEM in presence (G–I) or absence (D–F) of 0.1% (v/v) DMSO (vehicle). Cells exposed to: 3 μM A1254 (J–L); 9 μM A1254 (M–O); 1 mM dbcAMP (P–R). dbcAMP-differentiating cells exposed to A1254 3 μM (S–U) or 9 μM (V–X). After the treatments, cells were subjected to GFAP immunostaining (green) (A, D, G, J, M, P, S and V). DAPI-nuclear stain (blue) of the same field (B, E, H, K, N, Q, T and W). Merge for composite images (C, F, I, L, O, R, U and X). All the treatments were performed for 24 h under serum-free conditions in presence of 0.1% (v/v) DMSO used as vehicle for A1254. Scale bar = 50 μm. (For interpretation of color in Fig. 3, the reader is referred to the web version of this article.)

Article Snippet: 2.2 Cell cultures and treatments The C6 rat glial cell line [ ] (American Type Culture Collection, ATTC CCL-107) was cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Invitrogen, Life Technologies, Italy), 1.5 mM l -glutamine, 100 units/ml penicillin, and 100 μg/ml streptomycin under humidified atmosphere of 5% CO2 at 37 °C.

Techniques: Immunocytochemistry, Cell Culture, Immunostaining, Staining

Journal: Lipids in Health and Disease

Article Title: Contribution of Pyk2 pathway and reactive oxygen species (ROS) to the anti-cancer effects of eicosapentaenoic acid (EPA) in PC3 prostate cancer cells

doi: 10.1186/s12944-019-1122-4

Figure Lengend Snippet: Effect of EPA on EPA on PC3 ( a ) cell migration and ( b ) invasion. Double-chambered cell culture dishes with a transwell insert separating the two chambers were used to evaluate PC3 cell migration and invasion. Cells were seeded in the upper chamber, which was uncoated (migration) or coated (invasion) with Matrigel, while the lower chamber was filled with DMEM containing 10% FBS. Data represent mean + SEM ( n = 3). * P

Article Snippet: Cell culture PC3 cells (Riken BRC Cell Bank, Tsukuba, Japan) were grown in DMEM (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/mL of penicillin, and 100 μg/mL of streptomycin (Gibco).

Techniques: Migration, Cell Culture