heat inactivated endotoxin free fetal bovine serum fbs  (GE Healthcare)

 
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    GE Healthcare heat inactivated endotoxin free fetal bovine serum fbs
    Effects of Aβ on intracellular lysosomal pH value in primary microglia. Microglial cells were harvested and maintained in the 96-well plate, with <t>DMEM-F12</t> containing 2% <t>FBS</t> at least for 2 h for the return of a resting state. After treated with the indicated concentration of Aβ for the indicated time, microglia cells were rinsed with live cell imaging solution. Intracellular pH of microglia was detected using fluorescent dye-based pHrodo Red AM intracellular pH indicator. The wavelength of 560 and 580 nm fluorescent light for excitation and emission was used in a Gemini EM microtiter plate reader. Forskolin (100 μM), a powerful protein kinase A (PKA) activator, capable of enhancing lysosomal acidification in intact cell, was served as control. Intracellular pH values were determined by the value of the arbitrary pH ratios of light excited at 560 nm and emitted at 580 nm with pH calibration curve kit (Invitrogen). Data were collected from 3 independent experiments with 8-paralleling wells. * P
    Heat Inactivated Endotoxin Free Fetal Bovine Serum Fbs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heat inactivated endotoxin free fetal bovine serum fbs/product/GE Healthcare
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    heat inactivated endotoxin free fetal bovine serum fbs - by Bioz Stars, 2021-04
    97/100 stars

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    1) Product Images from "Amyloid β-Induced Redistribution of Transcriptional Factor EB and Lysosomal Dysfunction in Primary Microglial Cells"

    Article Title: Amyloid β-Induced Redistribution of Transcriptional Factor EB and Lysosomal Dysfunction in Primary Microglial Cells

    Journal: Frontiers in Aging Neuroscience

    doi: 10.3389/fnagi.2017.00228

    Effects of Aβ on intracellular lysosomal pH value in primary microglia. Microglial cells were harvested and maintained in the 96-well plate, with DMEM-F12 containing 2% FBS at least for 2 h for the return of a resting state. After treated with the indicated concentration of Aβ for the indicated time, microglia cells were rinsed with live cell imaging solution. Intracellular pH of microglia was detected using fluorescent dye-based pHrodo Red AM intracellular pH indicator. The wavelength of 560 and 580 nm fluorescent light for excitation and emission was used in a Gemini EM microtiter plate reader. Forskolin (100 μM), a powerful protein kinase A (PKA) activator, capable of enhancing lysosomal acidification in intact cell, was served as control. Intracellular pH values were determined by the value of the arbitrary pH ratios of light excited at 560 nm and emitted at 580 nm with pH calibration curve kit (Invitrogen). Data were collected from 3 independent experiments with 8-paralleling wells. * P
    Figure Legend Snippet: Effects of Aβ on intracellular lysosomal pH value in primary microglia. Microglial cells were harvested and maintained in the 96-well plate, with DMEM-F12 containing 2% FBS at least for 2 h for the return of a resting state. After treated with the indicated concentration of Aβ for the indicated time, microglia cells were rinsed with live cell imaging solution. Intracellular pH of microglia was detected using fluorescent dye-based pHrodo Red AM intracellular pH indicator. The wavelength of 560 and 580 nm fluorescent light for excitation and emission was used in a Gemini EM microtiter plate reader. Forskolin (100 μM), a powerful protein kinase A (PKA) activator, capable of enhancing lysosomal acidification in intact cell, was served as control. Intracellular pH values were determined by the value of the arbitrary pH ratios of light excited at 560 nm and emitted at 580 nm with pH calibration curve kit (Invitrogen). Data were collected from 3 independent experiments with 8-paralleling wells. * P

    Techniques Used: Concentration Assay, Live Cell Imaging

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    Article Snippet: Cell culture Rat glomerular MCs were obtained from China Center for Type Culture Collection (no. HBYZ-1; CCTCC, WuHan, CN). .. Cells were cultured in DMEM low glucose medium (Hyclone, Logan, UT) with 10 % heat-inactivated FBS (Hyclone, Logan, UT) and 1 % antibiotic solution (Sigma-Aldrich, St. Louis, MO). .. Monolayer rat glomerular MCs were rendered quiescent in DMEM low glucose medium containing 0 % fetal bovine serum for 24 h. After treated with or without 30 mM D-glucose, mannitol or L-glucose (Sigma-Aldrich, St. Louis, MO), the cells were harvested for the extraction of protein and RNA.

    Article Title: GADD34 inhibits activation-induced apoptosis of macrophages through enhancement of autophagy
    Article Snippet: RAW 264.7 cells were cultured in DMEM (Sigma, D5766) supplemented with 10% heat-inactivated FBS (Equitech-Bio Inc.). .. THP1 cells were cultured in RPMI1640 (Sigma, R8758) supplemented with 10% heat-inactivated FBS (HyClone). .. Bone marrow derived macrophages (BMDMs) were prepared from GADD34 KO mice or wild-type (WT) mice.

    Article Title: Induced myelomonocytic differentiation in leukemia cells is accompanied by noncanonical transcription factor expression
    Article Snippet: No human primary tissues were obtained or used in this study—all parent cell lines were established approximately 30 years ago and are purchasable from ATCC. .. Cells were cultured in RPMI 1640 medium (Invitrogen, Carlsbad, CA) supplemented with 5% (HL60) or 10% (K562, NB4, U937) heat-inactivated fetal bovine serum (FBS; Hyclone, Logan, UT) and 1% antibiotic–antimycotic (Invitrogen) and maintained in a 5% CO2 humidified environment at 37 °C. ..

    Article Title: Hypomethylation associated enhanced transcription of trefoil factor-3 mediates tamoxifen-stimulated oncogenicity of ER+ endometrial carcinoma cells
    Article Snippet: To generate an in vitro model of chronic TAM stimulation, Ishikawa and ECC-1 cells were first exposed to 0.5μM of TAM for six weeks and then to 1μM of TAM for twelve weeks. .. Ishikawa and ECC-1 cells were cultured in DMEM (Hyclone, USA) supplemented with 10% heat inactivated fetal bovine serum (FBS) (Hyclone, USA), 100 IU/mL penicillin and 100μg/mL streptomycin (Invitrogen, USA) as per ATCC propagation instructions. ..

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    Article Snippet: .. All cell lines were cultured in DMEM (Hyclone, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Hyclone, USA), 100 IU/mL penicillin and 100 μg/mL streptomycin (Invitrogen, USA) as recommended. .. Huh7, Hep3B and HepG2 cells with forced expression of TFF3 or siRNA-mediated depletion of TFF3 were transfected with FuGENE 6 transfection reagent (Promega, USA) as previously described [ ].

    Derivative Assay:

    Article Title: Unique induction of p21WAF1/CIP1expression by vinorelbine in androgen-independent prostate cancer cells
    Article Snippet: Cell culture LNCaP cells were purchased from the American Type Culture Collection (Rockville, MD, USA) and maintained in RPMI-1640 (GIBCO, Gaithersburg, MD, USA) containing 10% heat-inactivated bovine serum (FBS). .. The AI LNCaP subline derived from LNCaP cells was maintained in RPMI-1640 medium containing 10% charcoal-stripped, heat-inactivated FBS (CSFBS) (Hyclone Laboratories, Inc., Logan, UT, USA) and 5 μ g ml−1 of insulin as described previously ( ). ..

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    GE Healthcare heat inactivated endotoxin free fetal bovine serum fbs
    Effects of Aβ on intracellular lysosomal pH value in primary microglia. Microglial cells were harvested and maintained in the 96-well plate, with <t>DMEM-F12</t> containing 2% <t>FBS</t> at least for 2 h for the return of a resting state. After treated with the indicated concentration of Aβ for the indicated time, microglia cells were rinsed with live cell imaging solution. Intracellular pH of microglia was detected using fluorescent dye-based pHrodo Red AM intracellular pH indicator. The wavelength of 560 and 580 nm fluorescent light for excitation and emission was used in a Gemini EM microtiter plate reader. Forskolin (100 μM), a powerful protein kinase A (PKA) activator, capable of enhancing lysosomal acidification in intact cell, was served as control. Intracellular pH values were determined by the value of the arbitrary pH ratios of light excited at 560 nm and emitted at 580 nm with pH calibration curve kit (Invitrogen). Data were collected from 3 independent experiments with 8-paralleling wells. * P
    Heat Inactivated Endotoxin Free Fetal Bovine Serum Fbs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heat inactivated endotoxin free fetal bovine serum fbs/product/GE Healthcare
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    heat inactivated endotoxin free fetal bovine serum fbs - by Bioz Stars, 2021-04
    97/100 stars
    		  Buy from Supplier
    endotoxin free fetal bovine serum fbs  (GE Healthcare)
    97
    GE Healthcare endotoxin free fetal bovine serum fbs
    Effects of Aβ on intracellular lysosomal pH value in primary microglia. Microglial cells were harvested and maintained in the 96-well plate, with <t>DMEM-F12</t> containing 2% <t>FBS</t> at least for 2 h for the return of a resting state. After treated with the indicated concentration of Aβ for the indicated time, microglia cells were rinsed with live cell imaging solution. Intracellular pH of microglia was detected using fluorescent dye-based pHrodo Red AM intracellular pH indicator. The wavelength of 560 and 580 nm fluorescent light for excitation and emission was used in a Gemini EM microtiter plate reader. Forskolin (100 μM), a powerful protein kinase A (PKA) activator, capable of enhancing lysosomal acidification in intact cell, was served as control. Intracellular pH values were determined by the value of the arbitrary pH ratios of light excited at 560 nm and emitted at 580 nm with pH calibration curve kit (Invitrogen). Data were collected from 3 independent experiments with 8-paralleling wells. * P
    Endotoxin Free Fetal Bovine Serum Fbs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endotoxin free fetal bovine serum fbs/product/GE Healthcare
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    endotoxin free fetal bovine serum fbs - by Bioz Stars, 2021-04
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Effects of Aβ on intracellular lysosomal pH value in primary microglia. Microglial cells were harvested and maintained in the 96-well plate, with DMEM-F12 containing 2% FBS at least for 2 h for the return of a resting state. After treated with the indicated concentration of Aβ for the indicated time, microglia cells were rinsed with live cell imaging solution. Intracellular pH of microglia was detected using fluorescent dye-based pHrodo Red AM intracellular pH indicator. The wavelength of 560 and 580 nm fluorescent light for excitation and emission was used in a Gemini EM microtiter plate reader. Forskolin (100 μM), a powerful protein kinase A (PKA) activator, capable of enhancing lysosomal acidification in intact cell, was served as control. Intracellular pH values were determined by the value of the arbitrary pH ratios of light excited at 560 nm and emitted at 580 nm with pH calibration curve kit (Invitrogen). Data were collected from 3 independent experiments with 8-paralleling wells. * P

    Journal: Frontiers in Aging Neuroscience

    Article Title: Amyloid β-Induced Redistribution of Transcriptional Factor EB and Lysosomal Dysfunction in Primary Microglial Cells

    doi: 10.3389/fnagi.2017.00228

    Figure Lengend Snippet: Effects of Aβ on intracellular lysosomal pH value in primary microglia. Microglial cells were harvested and maintained in the 96-well plate, with DMEM-F12 containing 2% FBS at least for 2 h for the return of a resting state. After treated with the indicated concentration of Aβ for the indicated time, microglia cells were rinsed with live cell imaging solution. Intracellular pH of microglia was detected using fluorescent dye-based pHrodo Red AM intracellular pH indicator. The wavelength of 560 and 580 nm fluorescent light for excitation and emission was used in a Gemini EM microtiter plate reader. Forskolin (100 μM), a powerful protein kinase A (PKA) activator, capable of enhancing lysosomal acidification in intact cell, was served as control. Intracellular pH values were determined by the value of the arbitrary pH ratios of light excited at 560 nm and emitted at 580 nm with pH calibration curve kit (Invitrogen). Data were collected from 3 independent experiments with 8-paralleling wells. * P

    Article Snippet: DMEM-F12 and heat-inactivated endotoxin-free fetal bovine serum (FBS) were purchased from Hyclone (Logan, UT).

    Techniques: Concentration Assay, Live Cell Imaging