healthy peripheral blood mononuclear cells pbmcs  (Lonza)


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    Name:
    Human Peripheral Blood Mononuclear Cells
    Description:
    Mononuclear cells from human peripheral blood cryopreserved 10 million cells
    Catalog Number:
    4w-270
    Price:
    None
    Category:
    Primary and Stem Cells
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    Structured Review

    Lonza healthy peripheral blood mononuclear cells pbmcs
    Mononuclear cells from human peripheral blood cryopreserved 10 million cells
    https://www.bioz.com/result/healthy peripheral blood mononuclear cells pbmcs/product/Lonza
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    healthy peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2020-09
    99/100 stars

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    Centrifugation:

    Article Title: Porcupine Is Not Required for the Production of the Majority of Wnts from Primary Human Astrocytes and CD8+ T Cells
    Article Snippet: .. Culture and Activation of Human Peripheral Blood Mononuclear Cells (PBMCs) and Isolation of CD8+ T cells PBMCs were isolated from venous blood of healthy donors using lymphocyte separation medium (Lonza Biologics, Portsmouth, NH) and density centrifugation. .. PBMCs were then cultured at 1×106 cells mL of RPMI 1640, 10% heat-inactivated fetal bovine serum (FBS), 200 mM L-glutamine, and 1% penicillin/streptomycin (Lonza Biologics, Portsmouth, NH).

    In Vitro:

    Article Title: Kv1.3 Channel Blockade Modulates the Effector Function of B Cells in Granulomatosis with Polyangiitis
    Article Snippet: .. Briefly, peripheral blood mononuclear cells (PBMCs) were isolated from GPA patients, who produce ANCA upon in vitro induction , and stored in RPMI 1640 (Lonza, Basel, Switzerland) supplemented with 50 µg/mL gentamycin (GIBCO, Life Technologies, Grand Island, NY, USA), 10% fetal calf serum (FCS, Lonza), and 10% dimethyl sulfoxide (DMSO). ..

    Co-Culture Assay:

    Article Title: High expression of IDO1 and TGF-β1 during recurrence and post infection clearance with Chlamydia trachomatis, are independent of host IFN-γ response
    Article Snippet: .. Experiments were conducted in 48-well plates, where co-culture of 40,000 ECC1 cells/well and 8 × 105 female human PBMCs (Lonza, Australia) cells/well were seeded with RPMI-1640 medium (Sigma-Aldrich, Australia) containing 10% heat inactivated FCS (Life Technologies, Australia), 120 μg/ml streptomycin (Sigma-Aldrich, Australia), 50 μg/ml Gentamycin (Gibco, Australia), 37 °C, 5% CO2 . .. In addition, control plates with either ECC1 cells alone or human PBMCs alone were used.

    Isolation:

    Article Title: P2X7 Receptor Induces Tumor Necrosis Factor-α Converting Enzyme Activation and Release to Boost TNF-α Production
    Article Snippet: .. Human peripheral blood mononuclear cells were isolated following standard procedure ( ) and cultured for 16 h in RPMI 1640 medium (Lonza) with 10% of FCS, 2 mM Glutamax, and 100 U/ml penicillin–streptomycin (Life Technologies). .. After monocyte adherence, cells were washed and primed for 4 h with LPS (10 ng/ml), and then cells were washed or not with physiological buffer and incubated in the same buffer at 37°C with 3 mM of ATP for 20 min.

    Article Title: Porcupine Is Not Required for the Production of the Majority of Wnts from Primary Human Astrocytes and CD8+ T Cells
    Article Snippet: .. Culture and Activation of Human Peripheral Blood Mononuclear Cells (PBMCs) and Isolation of CD8+ T cells PBMCs were isolated from venous blood of healthy donors using lymphocyte separation medium (Lonza Biologics, Portsmouth, NH) and density centrifugation. .. PBMCs were then cultured at 1×106 cells mL of RPMI 1640, 10% heat-inactivated fetal bovine serum (FBS), 200 mM L-glutamine, and 1% penicillin/streptomycin (Lonza Biologics, Portsmouth, NH).

    Article Title: Kv1.3 Channel Blockade Modulates the Effector Function of B Cells in Granulomatosis with Polyangiitis
    Article Snippet: .. Briefly, peripheral blood mononuclear cells (PBMCs) were isolated from GPA patients, who produce ANCA upon in vitro induction , and stored in RPMI 1640 (Lonza, Basel, Switzerland) supplemented with 50 µg/mL gentamycin (GIBCO, Life Technologies, Grand Island, NY, USA), 10% fetal calf serum (FCS, Lonza), and 10% dimethyl sulfoxide (DMSO). ..

    Cell Culture:

    Article Title: P2X7 Receptor Induces Tumor Necrosis Factor-α Converting Enzyme Activation and Release to Boost TNF-α Production
    Article Snippet: .. Human peripheral blood mononuclear cells were isolated following standard procedure ( ) and cultured for 16 h in RPMI 1640 medium (Lonza) with 10% of FCS, 2 mM Glutamax, and 100 U/ml penicillin–streptomycin (Life Technologies). .. After monocyte adherence, cells were washed and primed for 4 h with LPS (10 ng/ml), and then cells were washed or not with physiological buffer and incubated in the same buffer at 37°C with 3 mM of ATP for 20 min.

    Activation Assay:

    Article Title: Porcupine Is Not Required for the Production of the Majority of Wnts from Primary Human Astrocytes and CD8+ T Cells
    Article Snippet: .. Culture and Activation of Human Peripheral Blood Mononuclear Cells (PBMCs) and Isolation of CD8+ T cells PBMCs were isolated from venous blood of healthy donors using lymphocyte separation medium (Lonza Biologics, Portsmouth, NH) and density centrifugation. .. PBMCs were then cultured at 1×106 cells mL of RPMI 1640, 10% heat-inactivated fetal bovine serum (FBS), 200 mM L-glutamine, and 1% penicillin/streptomycin (Lonza Biologics, Portsmouth, NH).

    other:

    Article Title: High expression of IDO1 and TGF-β1 during recurrence and post infection clearance with Chlamydia trachomatis, are independent of host IFN-γ response
    Article Snippet: In addition, control plates with either ECC1 cells alone or human PBMCs alone were used.

    Infection:

    Article Title: High expression of IDO1 and TGF-β1 during recurrence and post infection clearance with Chlamydia trachomatis, are independent of host IFN-γ response
    Article Snippet: .. In addition, infection of human PBMCs alone with live C. trachomatis , without the azithromycin treatment, resulted in significantly reduced, yet apparent infectivity (p < 0.0001). .. The number of chlamydial IFUs measured from ECC1 and PBMCs co-culture was significantly reduced in comparison to infected ECC1 culture alone (p < 0.0001) by almost two logs.

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  • 99
    Lonza healthy human peripheral blood
    Healthy Human Peripheral Blood, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/healthy human peripheral blood/product/Lonza
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    healthy human peripheral blood - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    pbmcs  (Lonza)
    93
    Lonza pbmcs
    Expression of CD107a after PMA and ionomycin stimulation on NK, NKT, and T cells from healthy donors is reduced by incubation with, CB-PPP, CB-PL, and CB-PR. Data represents analysis of <t>CD3–</t> CD56 dim, CD3– CD56 bright NK cells, CD3+ CD56+ NKT cells and CD3+ CD56– T cells in adult donor <t>PBMCs</t> ( n = 4) after incubation with CB-PRP derived samples or complete media only. Results show percentage of maximum expression relative to complete media for each cell type following 2 h stimulation with PMA and ionomycin. CB-PRP preparations investigated were cord blood platelet poor plasma (CB-PPP; n = 10), cord blood platelet lysate (CB-PL; n = 10), or cord blood platelet releasate (CB-PR; n = 10). PBMCs were incubated with each preparation diluted 50:50 with media and supplemented with IL-2, or with complete media and IL-2 only for 48 h prior to antibody staining and flow cytometry analysis. Each experiment was repeated with four different PBMC donors and data points represent donor means. Statistical analysis was performed using non-parametric one-way ANOVA (Kruskal-Wallis test with Dunn's post-hoc test for unpaired samples and Friedman's for paired samples). * p
    Pbmcs, supplied by Lonza, used in various techniques. Bioz Stars score: 93/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmcs/product/Lonza
    Average 93 stars, based on 168 article reviews
    Price from $9.99 to $1999.99
    pbmcs - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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    Expression of CD107a after PMA and ionomycin stimulation on NK, NKT, and T cells from healthy donors is reduced by incubation with, CB-PPP, CB-PL, and CB-PR. Data represents analysis of CD3– CD56 dim, CD3– CD56 bright NK cells, CD3+ CD56+ NKT cells and CD3+ CD56– T cells in adult donor PBMCs ( n = 4) after incubation with CB-PRP derived samples or complete media only. Results show percentage of maximum expression relative to complete media for each cell type following 2 h stimulation with PMA and ionomycin. CB-PRP preparations investigated were cord blood platelet poor plasma (CB-PPP; n = 10), cord blood platelet lysate (CB-PL; n = 10), or cord blood platelet releasate (CB-PR; n = 10). PBMCs were incubated with each preparation diluted 50:50 with media and supplemented with IL-2, or with complete media and IL-2 only for 48 h prior to antibody staining and flow cytometry analysis. Each experiment was repeated with four different PBMC donors and data points represent donor means. Statistical analysis was performed using non-parametric one-way ANOVA (Kruskal-Wallis test with Dunn's post-hoc test for unpaired samples and Friedman's for paired samples). * p

    Journal: Frontiers in Immunology

    Article Title: Cord Blood Platelet Rich Plasma Derivatives for Clinical Applications in Non-transfusion Medicine

    doi: 10.3389/fimmu.2020.00942

    Figure Lengend Snippet: Expression of CD107a after PMA and ionomycin stimulation on NK, NKT, and T cells from healthy donors is reduced by incubation with, CB-PPP, CB-PL, and CB-PR. Data represents analysis of CD3– CD56 dim, CD3– CD56 bright NK cells, CD3+ CD56+ NKT cells and CD3+ CD56– T cells in adult donor PBMCs ( n = 4) after incubation with CB-PRP derived samples or complete media only. Results show percentage of maximum expression relative to complete media for each cell type following 2 h stimulation with PMA and ionomycin. CB-PRP preparations investigated were cord blood platelet poor plasma (CB-PPP; n = 10), cord blood platelet lysate (CB-PL; n = 10), or cord blood platelet releasate (CB-PR; n = 10). PBMCs were incubated with each preparation diluted 50:50 with media and supplemented with IL-2, or with complete media and IL-2 only for 48 h prior to antibody staining and flow cytometry analysis. Each experiment was repeated with four different PBMC donors and data points represent donor means. Statistical analysis was performed using non-parametric one-way ANOVA (Kruskal-Wallis test with Dunn's post-hoc test for unpaired samples and Friedman's for paired samples). * p

    Article Snippet: For assessment of cellular function of CD3- CD56bright/dim NK cells, CD3+ CD56+ NKT cell and CD3+ CD56– T cells, healthy adult donor PBMCs were plated in RPMI (Lonza, Slough, UK) containing 10% heat-inactivated fetal calf serum (FCS) supplemented with 1% penicillin and streptomycin (complete media) and containing human IL-2.

    Techniques: Expressing, Incubation, Derivative Assay, Staining, Flow Cytometry

    Viability of CD3- CD56 dim, CD3- CD56 bright NK cells, CD3+ CD56+ NKT cells, and CD3+ CD56- T cells from adult donor PBMCs ( n = 3) after incubation with cord blood plasma preparations or complete media only. Results show percentage of live cells (Annexin V negative, 7-AAD negative) for each cell type. Cord blood plasma preparations investigated were cord blood platelet poor plasma (CB-PPP; n = 10), cord blood platelet lysate (CB-PL; n = 10), or cord blood platelet releasate (CB-PR; n = 10). PBMCs were incubated with each preparation diluted 50:50 with media and supplemented with IL-2, or with complete media and IL-2 only for 48 h prior to antibody staining and flow cytometry analysis. Each experiment was repeated with four different PBMC donors and data points represent donor means. Statistical analysis was performed using non-parametric one-way ANOVA (Kruskal-Wallis test with Dunn's post-hoc test for unpaired samples and Friedman's for paired samples). * p

    Journal: Frontiers in Immunology

    Article Title: Cord Blood Platelet Rich Plasma Derivatives for Clinical Applications in Non-transfusion Medicine

    doi: 10.3389/fimmu.2020.00942

    Figure Lengend Snippet: Viability of CD3- CD56 dim, CD3- CD56 bright NK cells, CD3+ CD56+ NKT cells, and CD3+ CD56- T cells from adult donor PBMCs ( n = 3) after incubation with cord blood plasma preparations or complete media only. Results show percentage of live cells (Annexin V negative, 7-AAD negative) for each cell type. Cord blood plasma preparations investigated were cord blood platelet poor plasma (CB-PPP; n = 10), cord blood platelet lysate (CB-PL; n = 10), or cord blood platelet releasate (CB-PR; n = 10). PBMCs were incubated with each preparation diluted 50:50 with media and supplemented with IL-2, or with complete media and IL-2 only for 48 h prior to antibody staining and flow cytometry analysis. Each experiment was repeated with four different PBMC donors and data points represent donor means. Statistical analysis was performed using non-parametric one-way ANOVA (Kruskal-Wallis test with Dunn's post-hoc test for unpaired samples and Friedman's for paired samples). * p

    Article Snippet: For assessment of cellular function of CD3- CD56bright/dim NK cells, CD3+ CD56+ NKT cell and CD3+ CD56– T cells, healthy adult donor PBMCs were plated in RPMI (Lonza, Slough, UK) containing 10% heat-inactivated fetal calf serum (FCS) supplemented with 1% penicillin and streptomycin (complete media) and containing human IL-2.

    Techniques: Incubation, Staining, Flow Cytometry

    Expression of NKG2D on NK, NKT and T cells from healthy donors is reduced by incubation with, CB-PPP, CB-PL, and CB-PR. Data represents analysis of CD3– CD56 dim, CD3– CD56 bright NK cells, CD3+ CD56+ NKT cells and CD3+ CD56– T cells in adult donor PBMCs ( n = 4) after incubation with CB-PRP preparations or complete media only. Results show percentage of maximum expression relative to complete media for each cell type from unstimulated cultures. CB-PRP preparations investigated were cord blood platelet poor plasma (CB-PPP; n = 10), cord blood platelet lysate (CB-PL; n = 10), or cord blood platelet releasate (CB-PR; n = 10). PBMCs were incubated with each preparation diluted 50:50 with media and supplemented with IL-2, or with complete media and IL-2 only for 48 h prior to antibody staining and flow cytometry analysis. Each experiment was repeated with four different PBMC donors and data points represent donor means. Statistical analysis was performed using non-parametric one-way ANOVA (Kruskal-Wallis test with Dunn's post-hoc test for unpaired samples and Friedman's for paired samples). * p

    Journal: Frontiers in Immunology

    Article Title: Cord Blood Platelet Rich Plasma Derivatives for Clinical Applications in Non-transfusion Medicine

    doi: 10.3389/fimmu.2020.00942

    Figure Lengend Snippet: Expression of NKG2D on NK, NKT and T cells from healthy donors is reduced by incubation with, CB-PPP, CB-PL, and CB-PR. Data represents analysis of CD3– CD56 dim, CD3– CD56 bright NK cells, CD3+ CD56+ NKT cells and CD3+ CD56– T cells in adult donor PBMCs ( n = 4) after incubation with CB-PRP preparations or complete media only. Results show percentage of maximum expression relative to complete media for each cell type from unstimulated cultures. CB-PRP preparations investigated were cord blood platelet poor plasma (CB-PPP; n = 10), cord blood platelet lysate (CB-PL; n = 10), or cord blood platelet releasate (CB-PR; n = 10). PBMCs were incubated with each preparation diluted 50:50 with media and supplemented with IL-2, or with complete media and IL-2 only for 48 h prior to antibody staining and flow cytometry analysis. Each experiment was repeated with four different PBMC donors and data points represent donor means. Statistical analysis was performed using non-parametric one-way ANOVA (Kruskal-Wallis test with Dunn's post-hoc test for unpaired samples and Friedman's for paired samples). * p

    Article Snippet: For assessment of cellular function of CD3- CD56bright/dim NK cells, CD3+ CD56+ NKT cell and CD3+ CD56– T cells, healthy adult donor PBMCs were plated in RPMI (Lonza, Slough, UK) containing 10% heat-inactivated fetal calf serum (FCS) supplemented with 1% penicillin and streptomycin (complete media) and containing human IL-2.

    Techniques: Expressing, Incubation, Staining, Flow Cytometry

    NK cells retain IL-2/IL-15 responsiveness and effector function, but an adaptive “memory-like” subset is lacking. (A) Flow cytometry plot of CD16 and CD56 expression in CD3 − CD19 − lymphocytes (patient B1), representative of four independent experiments. (B) Histograms of granzyme B and perforin content in CD56 bright versus CD56 dim NK cells. Healthy control in blue, patient B1 in red. Experiment displayed representative of three independent experiments. (C) STAT5 phosphorylation in NK cells (B1) in response to IL-2, IL-7, and IL-15 stimulation. (D) CD107a expression (degranulation) in healthy control and patient B1 NK cells co-cultured with K562 cells after 12 h of priming with IL-2 or IL-15 or left unprimed. (E) Percentage of 7-AAD–positive, i.e., dead, K562 cells as a measure of cytotoxicity when co-cultured with healthy control (blue circles) PBMCs or patient B1 (red squares) PBMCs. (F) IFNγ production in response to the indicated stimuli in control NK cells (blue circles) or patient B1 NK cells (red squares). (G) Expression of NKG2C and CD57 on NK cells of CMV + control and patient B1. (H) Summary graph displaying the percentage of CD57 + positivity within the NKG2C + and NKG2C − NK cell subsets (mean ± SD) in patient A1 (red triangles) and B1 (red squares). Data representative of six independent experiments. (I) FACS plots of FcεRIγ, PLZF, and SYK expression in CMV − and CMV + healthy controls and well as patient B1, gated on NKG2C-expressing NK cells. (J) Summary graphs showing the percentage of NKG2C + NK cells from patient A1 (red triangles) and B1 (red squares) down-regulating the indicated proteins. ****, P

    Journal: The Journal of Experimental Medicine

    Article Title: Human interleukin-2 receptor β mutations associated with defects in immunity and peripheral tolerance

    doi: 10.1084/jem.20182304

    Figure Lengend Snippet: NK cells retain IL-2/IL-15 responsiveness and effector function, but an adaptive “memory-like” subset is lacking. (A) Flow cytometry plot of CD16 and CD56 expression in CD3 − CD19 − lymphocytes (patient B1), representative of four independent experiments. (B) Histograms of granzyme B and perforin content in CD56 bright versus CD56 dim NK cells. Healthy control in blue, patient B1 in red. Experiment displayed representative of three independent experiments. (C) STAT5 phosphorylation in NK cells (B1) in response to IL-2, IL-7, and IL-15 stimulation. (D) CD107a expression (degranulation) in healthy control and patient B1 NK cells co-cultured with K562 cells after 12 h of priming with IL-2 or IL-15 or left unprimed. (E) Percentage of 7-AAD–positive, i.e., dead, K562 cells as a measure of cytotoxicity when co-cultured with healthy control (blue circles) PBMCs or patient B1 (red squares) PBMCs. (F) IFNγ production in response to the indicated stimuli in control NK cells (blue circles) or patient B1 NK cells (red squares). (G) Expression of NKG2C and CD57 on NK cells of CMV + control and patient B1. (H) Summary graph displaying the percentage of CD57 + positivity within the NKG2C + and NKG2C − NK cell subsets (mean ± SD) in patient A1 (red triangles) and B1 (red squares). Data representative of six independent experiments. (I) FACS plots of FcεRIγ, PLZF, and SYK expression in CMV − and CMV + healthy controls and well as patient B1, gated on NKG2C-expressing NK cells. (J) Summary graphs showing the percentage of NKG2C + NK cells from patient A1 (red triangles) and B1 (red squares) down-regulating the indicated proteins. ****, P

    Article Snippet: Flow cytometry–based STAT phosphorylation assay At the National Institutes of Health, PBMCs were thawed in XVIVO media (Lonza) with 10% human AB serum (Sigma-Aldrich), pelleted, washed with XVIVO, and resuspended in XVIVO media with 1% human AB serum at the concentration of 106 cells/ml.

    Techniques: Flow Cytometry, Cytometry, Expressing, Cell Culture, FACS