He4, supplied by Novoprotein, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Average 88 stars, based on 3 article reviews
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1) Product Images from "Kallikrein family proteases KLK6 and KLK7 are potential early detection and diagnostic biomarkers for serous and papillary serous ovarian cancer subtypes"
Article Title: Kallikrein family proteases KLK6 and KLK7 are potential early detection and diagnostic biomarkers for serous and papillary serous ovarian cancer subtypes
Journal: Journal of Ovarian Research
Figure Legend Snippet: Overexpression of KLK6 and KLK7 genes was observes in OVC cell lines. (A) Established OVC cell lines representing different ages, stages and subtypes were selected and tested by end-point PCR for expression of known OVC genes (CA-125, HE4, and CEA) relative to normal ovarian epithelial cell lines. Amplified cDNAs were qualitatively compared following electrophoretic separation as ethidium bromide-stained bands on agarose gels. (B) Gene expression of KLK6 and KLK7 in OVC cell lines (solid black bars) and normal ovary cell lines (N) was analyzed by qRT-PCR and normalized against a “primary-like” normal ovarian cell line (IOSE523; solid gray bar). IOSE523 cells begin to senesce after twenty passages while FIOSE118 cells are immortal. The mean fold change represents triplicate measurements, and standard error bars are shown.
Techniques Used: Over Expression, Polymerase Chain Reaction, Expressing, Amplification, Staining, Quantitative RT-PCR
Figure Legend Snippet: CA-125 and HE4 levels were both increased in only 58% of serum samples of early stage serous carcinoma patients as measured by ELISA. CA-125 (A) and HE4 (B) production was measured by ELISA. Levels of these proteins were calculated as averages of triplicates. The line across the figures is the low threshold for protein up-regulation. An asterisk (*) indicates that this particular patient demonstrated no up-regulation in both CA-125 and HE4.
Techniques Used: Enzyme-linked Immunosorbent Assay
2) Product Images from "HIV-Enhancing Factors Are Secreted by Reproductive Epithelia upon Inoculation with Bacterial Vaginosis-Associated Bacteria"
Article Title: HIV-Enhancing Factors Are Secreted by Reproductive Epithelia upon Inoculation with Bacterial Vaginosis-Associated Bacteria
Journal: Protein and peptide letters
Figure Legend Snippet: Several Proteins are Upregulated by End1 Epithelia in Response to A. vaginae stimulation The following volumes of CM were concentrated, resolved, and immunoblotted for protein detection: 300 µl for Lipocalin 2; 400 µl for Cyclophilin A; 450 µl for Trappin-2 and 2000 µl for HE4. Recombinant standards (labeled as ng/lane) were resolved alongside CM to estimate protein content. One representative of three independent experiments for each analyte is shown.
Techniques Used: Recombinant, Labeling
Figure Legend Snippet: Recombinant Proteins Enhance HIV Infection in the Presence of A. vaginae -Inoculated End1 CM Recombinant proteins lipocalin 2, cyclophilin A, trappin-2 or HE4 were added to the TZM-bl reporter assay at 0.1–100 ng/ml, in the presence of A) mock-inoculated End1 CM, or B) A. vaginae -inoculated End1 CM. CM was added at 4X concentration in all conditions. Asterisks (*=p
Techniques Used: Recombinant, Infection, Reporter Assay, Concentration Assay