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Toyobo hcv rna
Hcv Rna, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hcv rna/product/Toyobo
Average 92 stars, based on 4 article reviews
Price from $9.99 to $1999.99
hcv rna - by Bioz Stars, 2020-05
92/100 stars

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Polymerase Chain Reaction:

Article Title: Impact of IL-10 (-1082) Promoter-Single Nucleotide Polymorphism on the Outcome of Hepatitis C Virus Genotype 4 Infection
Article Snippet: .. Patients were treated by either pegylated or short-acting interferon along with ribavirin, (ii) 14 HCV RNA− spontaneously resolved patients (self-limiting HCV infection), patients had at least three negative HCV RNA PCR, six months apart with positive HCV-Ab on many occasions. .. The resolved HCV cases were identified as being negative for circulating HCV RNA and positive for the confirmatory test for the presence of anti-HCV antibodies by use of enzyme linked immunosorbent assay (ELISA) 3rd generation (CTK-Biotech, USA) according to the manufacturer’s protocols.

Real-time Polymerase Chain Reaction:

Article Title: Oxidative status and the response to pegylated-interferon alpha2a plus ribavirin in chronic genotype 4 HCV hepatitis
Article Snippet: .. HCV RNA was detected by commercially available Toyobo RNA-direct real time PCR kit on SLAN Real Time PCR Detection System, LG Lifescience, Korea. .. The HCV genotype was defined by the reverse line probe assay (INNO-LIPA v.1.0, innogenetics, Ghent, Belgium) according to the manufacturer's instruction.

Article Title: Interactive Effects of Immunoglobulin Gamma and Human Leucocyte Antigen Genotypes on Response to Interferon Based Therapy of Hepatitis C Virus
Article Snippet: .. HCV RNA was detected by commercially available Toyobo RNA-direct real time PCR kit on SLAN Real Time PCR Detection System, LG Lifescience, Korea. .. The HCV genotype was defined by the reverse line probe assay (INNO-LIPA v.1.0, innogenetics, Ghent, Belgium) according to the manufacturer’s instruction.

Article Title: Impact of IL-10 (-1082) Promoter-Single Nucleotide Polymorphism on the Outcome of Hepatitis C Virus Genotype 4 Infection
Article Snippet: .. Patients were grouped according to the outcome of HCV infection as follows: (1) 50 HCV RNA+ (chronic non-responder HCV) patients, having circulating HCV RNA detected by Toyobo RNA-direct real-time PCR on light cycler-LG Lifescience, Korea, and (2) 50 HCV RNA− (resolved HCV) cases, that were divided into (i) 36 HCV RNA− sustained responder patients who had a negative HCV RNA six months after completing 48 weeks therapy. .. Patients were treated by either pegylated or short-acting interferon along with ribavirin, (ii) 14 HCV RNA− spontaneously resolved patients (self-limiting HCV infection), patients had at least three negative HCV RNA PCR, six months apart with positive HCV-Ab on many occasions.

Article Title: Impact of Apo E gene polymorphism on HCV therapy related outcome in a cohort of HCV Egyptian patients
Article Snippet: .. HCV RNA was determined by Toyobo RNA-direct real time PCR kit on SLAN Real Time PCR Detection System, LG Lifescience, Korea. .. The HCV genotype was defined by the reverse line probe assay (INNO-LIPA v.1.0, innogenetics, Ghent, Belgium) according to the manufacturer’s instructions.

Article Title: Impact of IL-10 (-1082) Promoter-Single Nucleotide Polymorphism on the Outcome of Hepatitis C Virus Genotype 4 Infection
Article Snippet: .. For detection of the presence of hepatitis C viral particles, HCV RNA was detected by commercially available Toyobo RNA-direct real-time PCR kit on SLAN Real-Time PCR Detection System, LG Lifescience, Korea. .. For genotyping of HCV, a reverse line probe assay, GEN-C Reverse Hybridization Strip Assay (NLM diagnostic, Cat. no. AC004, Italy) was used, in which amplicons obtained through RT-PCR of HCV RNA 5′-UTR region isolated from the patient’s plasma and a volume of denaturing solution equal to that of the amplicon to be used for typing (30 μL) were mixed with hybridization buffer prewarmed to 45°C.

Infection:

Article Title: Impact of IL-10 (-1082) Promoter-Single Nucleotide Polymorphism on the Outcome of Hepatitis C Virus Genotype 4 Infection
Article Snippet: .. Patients were treated by either pegylated or short-acting interferon along with ribavirin, (ii) 14 HCV RNA− spontaneously resolved patients (self-limiting HCV infection), patients had at least three negative HCV RNA PCR, six months apart with positive HCV-Ab on many occasions. .. The resolved HCV cases were identified as being negative for circulating HCV RNA and positive for the confirmatory test for the presence of anti-HCV antibodies by use of enzyme linked immunosorbent assay (ELISA) 3rd generation (CTK-Biotech, USA) according to the manufacturer’s protocols.

Article Title: Impact of IL-10 (-1082) Promoter-Single Nucleotide Polymorphism on the Outcome of Hepatitis C Virus Genotype 4 Infection
Article Snippet: .. Patients were grouped according to the outcome of HCV infection as follows: (1) 50 HCV RNA+ (chronic non-responder HCV) patients, having circulating HCV RNA detected by Toyobo RNA-direct real-time PCR on light cycler-LG Lifescience, Korea, and (2) 50 HCV RNA− (resolved HCV) cases, that were divided into (i) 36 HCV RNA− sustained responder patients who had a negative HCV RNA six months after completing 48 weeks therapy. .. Patients were treated by either pegylated or short-acting interferon along with ribavirin, (ii) 14 HCV RNA− spontaneously resolved patients (self-limiting HCV infection), patients had at least three negative HCV RNA PCR, six months apart with positive HCV-Ab on many occasions.

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    Toyobo hcv rna levels
    Six representative conserved PQS sites, CD and Tm melting curves. ( A ) Six conserved PQS (core/E1/NS3/NS4B/NS5A/NS5B) sites of the <t>HCV</t> 1a H77 genome. ( B ) Synthetic <t>RNA</t> PQS samples (8.0 μM) annealed in 10 mM Tris–HCl buffer (pH 7.0) containing 100 mM KCl were heated to 90°C and cooled to RT at a rate of 1°C min –1 . The CD spectra were recorded at 25°C with a scan range from 320 to 200 nm. ( C ) The T m thermal stability of each G4 RNAs was measured by recording the molar ellipticity at 264 nm as a function of temperature. Tm values for each oligonucleotide were calculated as described in Materials and Methods.
    Hcv Rna Levels, supplied by Toyobo, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcv rna levels/product/Toyobo
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    hcv rna levels - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

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    Six representative conserved PQS sites, CD and Tm melting curves. ( A ) Six conserved PQS (core/E1/NS3/NS4B/NS5A/NS5B) sites of the HCV 1a H77 genome. ( B ) Synthetic RNA PQS samples (8.0 μM) annealed in 10 mM Tris–HCl buffer (pH 7.0) containing 100 mM KCl were heated to 90°C and cooled to RT at a rate of 1°C min –1 . The CD spectra were recorded at 25°C with a scan range from 320 to 200 nm. ( C ) The T m thermal stability of each G4 RNAs was measured by recording the molar ellipticity at 264 nm as a function of temperature. Tm values for each oligonucleotide were calculated as described in Materials and Methods.

    Journal: Nucleic Acids Research

    Article Title: Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication

    doi: 10.1093/nar/gky1177

    Figure Lengend Snippet: Six representative conserved PQS sites, CD and Tm melting curves. ( A ) Six conserved PQS (core/E1/NS3/NS4B/NS5A/NS5B) sites of the HCV 1a H77 genome. ( B ) Synthetic RNA PQS samples (8.0 μM) annealed in 10 mM Tris–HCl buffer (pH 7.0) containing 100 mM KCl were heated to 90°C and cooled to RT at a rate of 1°C min –1 . The CD spectra were recorded at 25°C with a scan range from 320 to 200 nm. ( C ) The T m thermal stability of each G4 RNAs was measured by recording the molar ellipticity at 264 nm as a function of temperature. Tm values for each oligonucleotide were calculated as described in Materials and Methods.

    Article Snippet: The relative mRNA expression of HCV RNA levels at 5′ UTR and NCL mRNA were quantitated using RT-qPCR with the SYBR Green real-time PCR Master Mix kit (Toyobo, Osaka, Japan).

    Techniques:

    NCL-R 3–4 C can stabilize target core RNA G4 and inhibit the trap by the corresponding ASO. ( A ) Schematic depiction of the inhibition of FRET. ( B ) Time course of the fluorescence intensity ( F – F 0 ) of a dual-labelled probe with increasing amounts of NCL-R 3–4 C. The final concentrations of HCV core RNA G4-dual and AS-HCV core RNA G4 were 200 nM and 2.0 mM, respectively. F – F 0 = (real time fluorescence intensity – initial fluorescence intensity)/initial fluorescence intensity. ( C ) G4 structure of HCV core RNA G4 evidenced by 1 H NMR. The final concentrations of HCV core RNA G4 and ASO were 0.5 and 0.1 mM, respectively.

    Journal: Nucleic Acids Research

    Article Title: Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication

    doi: 10.1093/nar/gky1177

    Figure Lengend Snippet: NCL-R 3–4 C can stabilize target core RNA G4 and inhibit the trap by the corresponding ASO. ( A ) Schematic depiction of the inhibition of FRET. ( B ) Time course of the fluorescence intensity ( F – F 0 ) of a dual-labelled probe with increasing amounts of NCL-R 3–4 C. The final concentrations of HCV core RNA G4-dual and AS-HCV core RNA G4 were 200 nM and 2.0 mM, respectively. F – F 0 = (real time fluorescence intensity – initial fluorescence intensity)/initial fluorescence intensity. ( C ) G4 structure of HCV core RNA G4 evidenced by 1 H NMR. The final concentrations of HCV core RNA G4 and ASO were 0.5 and 0.1 mM, respectively.

    Article Snippet: The relative mRNA expression of HCV RNA levels at 5′ UTR and NCL mRNA were quantitated using RT-qPCR with the SYBR Green real-time PCR Master Mix kit (Toyobo, Osaka, Japan).

    Techniques: Allele-specific Oligonucleotide, Inhibition, Fluorescence, Nuclear Magnetic Resonance

    The effects of G4 ligands on the colocalization of NCL and HCV core RNA G4 in Huh7.5.1 cells by confocal immunofluorescence analysis. Confocal immunofluorescence images with magnification 63×.

    Journal: Nucleic Acids Research

    Article Title: Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication

    doi: 10.1093/nar/gky1177

    Figure Lengend Snippet: The effects of G4 ligands on the colocalization of NCL and HCV core RNA G4 in Huh7.5.1 cells by confocal immunofluorescence analysis. Confocal immunofluorescence images with magnification 63×.

    Article Snippet: The relative mRNA expression of HCV RNA levels at 5′ UTR and NCL mRNA were quantitated using RT-qPCR with the SYBR Green real-time PCR Master Mix kit (Toyobo, Osaka, Japan).

    Techniques: Immunofluorescence

    Direct interaction and colocalization between HCV core RNA G4 and NCL in Huh7.5.1 cells. ( A ) RT-qPCR and Western blot analysis of HCV RNA and core protein expression. ( B ) G4 pull-down and Western blot. The fourth lane represents cell lysates directly used for western blot. β-actin was used as an internal control. ( C ) Colocalization of HCV core RNA G4 with NCL in Huh7.5.1 cells by confocal immunofluorescence. ( D ) Analysis of colocalization of wild-type HCV and modified G4 HCV with NCL in Huh7.5.1 cells by confocal immunofluorescence. Images in C and D with magnification 63×.

    Journal: Nucleic Acids Research

    Article Title: Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication

    doi: 10.1093/nar/gky1177

    Figure Lengend Snippet: Direct interaction and colocalization between HCV core RNA G4 and NCL in Huh7.5.1 cells. ( A ) RT-qPCR and Western blot analysis of HCV RNA and core protein expression. ( B ) G4 pull-down and Western blot. The fourth lane represents cell lysates directly used for western blot. β-actin was used as an internal control. ( C ) Colocalization of HCV core RNA G4 with NCL in Huh7.5.1 cells by confocal immunofluorescence. ( D ) Analysis of colocalization of wild-type HCV and modified G4 HCV with NCL in Huh7.5.1 cells by confocal immunofluorescence. Images in C and D with magnification 63×.

    Article Snippet: The relative mRNA expression of HCV RNA levels at 5′ UTR and NCL mRNA were quantitated using RT-qPCR with the SYBR Green real-time PCR Master Mix kit (Toyobo, Osaka, Japan).

    Techniques: Quantitative RT-PCR, Western Blot, Expressing, Immunofluorescence, Modification