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PrimerDesign Inc hcv rna
<t>HCV</t> <t>RNA</t> Load in Brain and Liver Tissue
Hcv Rna, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier"

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

Journal: Gastroenterology

doi: 10.1053/j.gastro.2011.11.028

HCV RNA Load in Brain and Liver Tissue
Figure Legend Snippet: HCV RNA Load in Brain and Liver Tissue

Techniques Used:

HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were
Figure Legend Snippet: HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

Techniques Used: Expressing, Infection

2) Product Images from "Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier"

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

Journal: Gastroenterology

doi: 10.1053/j.gastro.2011.11.028

HCV RNA Load in Brain and Liver Tissue
Figure Legend Snippet: HCV RNA Load in Brain and Liver Tissue

Techniques Used:

HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were
Figure Legend Snippet: HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

Techniques Used: Expressing, Infection

3) Product Images from "Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier"

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

Journal: Gastroenterology

doi: 10.1053/j.gastro.2011.11.028

HCV RNA Load in Brain and Liver Tissue
Figure Legend Snippet: HCV RNA Load in Brain and Liver Tissue

Techniques Used:

HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were
Figure Legend Snippet: HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

Techniques Used: Expressing, Infection

4) Product Images from "Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier"

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

Journal: Gastroenterology

doi: 10.1053/j.gastro.2011.11.028

HCV RNA Load in Brain and Liver Tissue
Figure Legend Snippet: HCV RNA Load in Brain and Liver Tissue

Techniques Used:

HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were
Figure Legend Snippet: HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

Techniques Used: Expressing, Infection

5) Product Images from "Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier"

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

Journal: Gastroenterology

doi: 10.1053/j.gastro.2011.11.028

HCV RNA Load in Brain and Liver Tissue
Figure Legend Snippet: HCV RNA Load in Brain and Liver Tissue

Techniques Used:

HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were
Figure Legend Snippet: HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

Techniques Used: Expressing, Infection

6) Product Images from "Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier"

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

Journal: Gastroenterology

doi: 10.1053/j.gastro.2011.11.028

HCV RNA Load in Brain and Liver Tissue
Figure Legend Snippet: HCV RNA Load in Brain and Liver Tissue

Techniques Used:

HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were
Figure Legend Snippet: HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

Techniques Used: Expressing, Infection

7) Product Images from "Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier"

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

Journal: Gastroenterology

doi: 10.1053/j.gastro.2011.11.028

HCV RNA Load in Brain and Liver Tissue
Figure Legend Snippet: HCV RNA Load in Brain and Liver Tissue

Techniques Used:

HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were
Figure Legend Snippet: HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

Techniques Used: Expressing, Infection

8) Product Images from "Hepatitis C virus infection of neuroepithelioma cell lines"

Article Title: Hepatitis C virus infection of neuroepithelioma cell lines

Journal: Gastroenterology

doi: 10.1053/j.gastro.2010.06.008

HCVcc infection of hepatoma and neuroepithelioma cells HCVcc J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T cells for 12h. Unbound virus was removed by washing and the infection measured 72h later by quantifying focus forming units/ml (FFU/ml) (A). Representative images of NS5A + J6/JFH infected Huh-7.5, MC-IXC and SK-PN-DW cells denoting comparable levels of NS5A expression, where the scale bar represents 10μm. (B) Cellular RNA was extracted from uninfected (UF) and J6/JFH infected cells after 12h and 72h in the presence or absence of anti-NS3 VX-950 (10μg/ml) or Interferon-α (IFN-α, 100 IU/ml) and the level of HCV RNA copies assessed by qPCR. (C) J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T for 12h, unbound virus removed by washing and the cells lysed for RNA purification after 48h (white), 72h (light grey) and 96h (dark grey). HCV RNA levels significantly increased between 48 and 72h post infection for all cell types tested. Cells were treated with anti-CD81 2s131 at 5μg/ml and HCV RNA copies assessed after 96h (black). Statistical comparisons were made using student's t-test, where * p
Figure Legend Snippet: HCVcc infection of hepatoma and neuroepithelioma cells HCVcc J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T cells for 12h. Unbound virus was removed by washing and the infection measured 72h later by quantifying focus forming units/ml (FFU/ml) (A). Representative images of NS5A + J6/JFH infected Huh-7.5, MC-IXC and SK-PN-DW cells denoting comparable levels of NS5A expression, where the scale bar represents 10μm. (B) Cellular RNA was extracted from uninfected (UF) and J6/JFH infected cells after 12h and 72h in the presence or absence of anti-NS3 VX-950 (10μg/ml) or Interferon-α (IFN-α, 100 IU/ml) and the level of HCV RNA copies assessed by qPCR. (C) J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T for 12h, unbound virus removed by washing and the cells lysed for RNA purification after 48h (white), 72h (light grey) and 96h (dark grey). HCV RNA levels significantly increased between 48 and 72h post infection for all cell types tested. Cells were treated with anti-CD81 2s131 at 5μg/ml and HCV RNA copies assessed after 96h (black). Statistical comparisons were made using student's t-test, where * p

Techniques Used: Infection, Expressing, Real-time Polymerase Chain Reaction, Purification

9) Product Images from "Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier"

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

Journal: Gastroenterology

doi: 10.1053/j.gastro.2011.11.028

HCV RNA Load in Brain and Liver Tissue
Figure Legend Snippet: HCV RNA Load in Brain and Liver Tissue

Techniques Used:

HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were
Figure Legend Snippet: HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

Techniques Used: Expressing, Infection

10) Product Images from "Hepatitis C virus infection of neuroepithelioma cell lines"

Article Title: Hepatitis C virus infection of neuroepithelioma cell lines

Journal: Gastroenterology

doi: 10.1053/j.gastro.2010.06.008

HCVcc infection of hepatoma and neuroepithelioma cells HCVcc J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T cells for 12h. Unbound virus was removed by washing and the infection measured 72h later by quantifying focus forming units/ml (FFU/ml) (A). Representative images of NS5A + J6/JFH infected Huh-7.5, MC-IXC and SK-PN-DW cells denoting comparable levels of NS5A expression, where the scale bar represents 10μm. (B) Cellular RNA was extracted from uninfected (UF) and J6/JFH infected cells after 12h and 72h in the presence or absence of anti-NS3 VX-950 (10μg/ml) or Interferon-α (IFN-α, 100 IU/ml) and the level of HCV RNA copies assessed by qPCR. (C) J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T for 12h, unbound virus removed by washing and the cells lysed for RNA purification after 48h (white), 72h (light grey) and 96h (dark grey). HCV RNA levels significantly increased between 48 and 72h post infection for all cell types tested. Cells were treated with anti-CD81 2s131 at 5μg/ml and HCV RNA copies assessed after 96h (black). Statistical comparisons were made using student's t-test, where * p
Figure Legend Snippet: HCVcc infection of hepatoma and neuroepithelioma cells HCVcc J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T cells for 12h. Unbound virus was removed by washing and the infection measured 72h later by quantifying focus forming units/ml (FFU/ml) (A). Representative images of NS5A + J6/JFH infected Huh-7.5, MC-IXC and SK-PN-DW cells denoting comparable levels of NS5A expression, where the scale bar represents 10μm. (B) Cellular RNA was extracted from uninfected (UF) and J6/JFH infected cells after 12h and 72h in the presence or absence of anti-NS3 VX-950 (10μg/ml) or Interferon-α (IFN-α, 100 IU/ml) and the level of HCV RNA copies assessed by qPCR. (C) J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T for 12h, unbound virus removed by washing and the cells lysed for RNA purification after 48h (white), 72h (light grey) and 96h (dark grey). HCV RNA levels significantly increased between 48 and 72h post infection for all cell types tested. Cells were treated with anti-CD81 2s131 at 5μg/ml and HCV RNA copies assessed after 96h (black). Statistical comparisons were made using student's t-test, where * p

Techniques Used: Infection, Expressing, Real-time Polymerase Chain Reaction, Purification

11) Product Images from "Paracrine Signals From Liver Sinusoidal Endothelium Regulate Hepatitis C Virus Replication"

Article Title: Paracrine Signals From Liver Sinusoidal Endothelium Regulate Hepatitis C Virus Replication

Journal: Hepatology (Baltimore, Md.)

doi: 10.1002/hep.26571

Elevated BMP4 in chronic liver disease. Liver biopsy protein lysates (30 μg) (A) or RNA (B,C) from normal, HCV-infected or alcohol-related liver disease (each n = 6) were immunoblotted for BMP4 (A) and qRT-PCR used to quantify BMP4 expression (B). Western blot depicts three representative samples and BMP4 expression is shown relative to normal samples and represents the mean value of all samples. Statistical comparison was made using the Kruskal-Wallis test with Dunn's correction where * P
Figure Legend Snippet: Elevated BMP4 in chronic liver disease. Liver biopsy protein lysates (30 μg) (A) or RNA (B,C) from normal, HCV-infected or alcohol-related liver disease (each n = 6) were immunoblotted for BMP4 (A) and qRT-PCR used to quantify BMP4 expression (B). Western blot depicts three representative samples and BMP4 expression is shown relative to normal samples and represents the mean value of all samples. Statistical comparison was made using the Kruskal-Wallis test with Dunn's correction where * P

Techniques Used: Infection, Quantitative RT-PCR, Expressing, Western Blot

LSEC secreted factors increase hepatocellular HCV replication. Conditioned media (CM) was collected from LSEC seeded at 4 × 10 4 /cm 2 for 24 hours in the absence of VEGF-A and was diluted 1:2 with fresh media and used to treat Huh-7.5 (A), or PHH (B) for 18 hours prior to infecting with HCV JFH-1. CM was replenished following infection. HCV infection was enumerated by quantifying NS5A expressing cells or HCV RNA levels as indicated and the data expressed relative to mock-treated cells. In replicate experiments with Huh-7.5, the number of cells following mock or CM treatment was quantified after 48 hours (C). Huh-7.5 supporting a JFH-1 subgenomic replicon were treated with CM for 48 hours and HCV RNA quantified (D). Data are mean ±1 SD of n = 4 donor LSEC CM. Statistical comparisons were made with the Mann-Whitney U test where * P
Figure Legend Snippet: LSEC secreted factors increase hepatocellular HCV replication. Conditioned media (CM) was collected from LSEC seeded at 4 × 10 4 /cm 2 for 24 hours in the absence of VEGF-A and was diluted 1:2 with fresh media and used to treat Huh-7.5 (A), or PHH (B) for 18 hours prior to infecting with HCV JFH-1. CM was replenished following infection. HCV infection was enumerated by quantifying NS5A expressing cells or HCV RNA levels as indicated and the data expressed relative to mock-treated cells. In replicate experiments with Huh-7.5, the number of cells following mock or CM treatment was quantified after 48 hours (C). Huh-7.5 supporting a JFH-1 subgenomic replicon were treated with CM for 48 hours and HCV RNA quantified (D). Data are mean ±1 SD of n = 4 donor LSEC CM. Statistical comparisons were made with the Mann-Whitney U test where * P

Techniques Used: Infection, Expressing, MANN-WHITNEY

Endothelial BMP4 expression is regulated by way of VEGFR-2. LSEC were starved of VEGF overnight and stimulated with VEGF-A, PlGF, or VEGF-E (all 10 ng/mL) for 8 hours, lysed, and RNA prepared for qRT-PCR analysis of BMP4 mRNA (A). Conditioned media (CM) from LSEC treated with the above ligands was collected and used to treat Huh-7.5 cells for 18 hours prior to infecting with HCV JFH-1 (B). Data are mean ±1 SD of n = 4 donor LSEC. Statistical comparisons were made with the Kruskal-Wallis test with Dunn's correction as appropriate and where ** P
Figure Legend Snippet: Endothelial BMP4 expression is regulated by way of VEGFR-2. LSEC were starved of VEGF overnight and stimulated with VEGF-A, PlGF, or VEGF-E (all 10 ng/mL) for 8 hours, lysed, and RNA prepared for qRT-PCR analysis of BMP4 mRNA (A). Conditioned media (CM) from LSEC treated with the above ligands was collected and used to treat Huh-7.5 cells for 18 hours prior to infecting with HCV JFH-1 (B). Data are mean ±1 SD of n = 4 donor LSEC. Statistical comparisons were made with the Kruskal-Wallis test with Dunn's correction as appropriate and where ** P

Techniques Used: Expressing, Quantitative RT-PCR

BMP4 is negatively regulated by VEGF and stimulates HCV replication. LSEC were starved of VEGF overnight and stimulated with VEGF-A as indicated for 8 hours and lysed to quantify BMP4 mRNA (A) or protein (B). Conditioned media (CM) from untreated or VEGF-A treated LSEC were incubated with a neutralizing anti-BMP4 antibody (10 μg/mL) and screened for its effect on Huh-7.5 permissivity (C). Huh-7.5 were treated with recombinant BMP4 for 48 hours and the cells assessed for their proliferative capacity (D) and permissivity to support HCV JFH-1 infection. Infection was enumerated by quantifying NS5A-expressing cells (E) or HCV RNA (F). Primary hepatocytes were treated with BMP4 (100 ng/mL) for 18 hours, inoculated with HCV JFH-1, and infection assessed by measuring HCV RNA (G). Data are mean ±1 SD of n = 4 donor LSEC. Statistical comparisons were made with the Kruskal-Wallis test with Dunn's correction (A,C,E,F), or the Mann-Whitney U test (G) and where * P
Figure Legend Snippet: BMP4 is negatively regulated by VEGF and stimulates HCV replication. LSEC were starved of VEGF overnight and stimulated with VEGF-A as indicated for 8 hours and lysed to quantify BMP4 mRNA (A) or protein (B). Conditioned media (CM) from untreated or VEGF-A treated LSEC were incubated with a neutralizing anti-BMP4 antibody (10 μg/mL) and screened for its effect on Huh-7.5 permissivity (C). Huh-7.5 were treated with recombinant BMP4 for 48 hours and the cells assessed for their proliferative capacity (D) and permissivity to support HCV JFH-1 infection. Infection was enumerated by quantifying NS5A-expressing cells (E) or HCV RNA (F). Primary hepatocytes were treated with BMP4 (100 ng/mL) for 18 hours, inoculated with HCV JFH-1, and infection assessed by measuring HCV RNA (G). Data are mean ±1 SD of n = 4 donor LSEC. Statistical comparisons were made with the Kruskal-Wallis test with Dunn's correction (A,C,E,F), or the Mann-Whitney U test (G) and where * P

Techniques Used: Incubation, Recombinant, Infection, Expressing, MANN-WHITNEY

12) Product Images from "Hepatitis C virus infection of neuroepithelioma cell lines"

Article Title: Hepatitis C virus infection of neuroepithelioma cell lines

Journal: Gastroenterology

doi: 10.1053/j.gastro.2010.06.008

HCVcc infection of hepatoma and neuroepithelioma cells HCVcc J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T cells for 12h. Unbound virus was removed by washing and the infection measured 72h later by quantifying focus forming units/ml (FFU/ml) (A). Representative images of NS5A + J6/JFH infected Huh-7.5, MC-IXC and SK-PN-DW cells denoting comparable levels of NS5A expression, where the scale bar represents 10μm. (B) Cellular RNA was extracted from uninfected (UF) and J6/JFH infected cells after 12h and 72h in the presence or absence of anti-NS3 VX-950 (10μg/ml) or Interferon-α (IFN-α, 100 IU/ml) and the level of HCV RNA copies assessed by qPCR. (C) J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T for 12h, unbound virus removed by washing and the cells lysed for RNA purification after 48h (white), 72h (light grey) and 96h (dark grey). HCV RNA levels significantly increased between 48 and 72h post infection for all cell types tested. Cells were treated with anti-CD81 2s131 at 5μg/ml and HCV RNA copies assessed after 96h (black). Statistical comparisons were made using student's t-test, where * p
Figure Legend Snippet: HCVcc infection of hepatoma and neuroepithelioma cells HCVcc J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T cells for 12h. Unbound virus was removed by washing and the infection measured 72h later by quantifying focus forming units/ml (FFU/ml) (A). Representative images of NS5A + J6/JFH infected Huh-7.5, MC-IXC and SK-PN-DW cells denoting comparable levels of NS5A expression, where the scale bar represents 10μm. (B) Cellular RNA was extracted from uninfected (UF) and J6/JFH infected cells after 12h and 72h in the presence or absence of anti-NS3 VX-950 (10μg/ml) or Interferon-α (IFN-α, 100 IU/ml) and the level of HCV RNA copies assessed by qPCR. (C) J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T for 12h, unbound virus removed by washing and the cells lysed for RNA purification after 48h (white), 72h (light grey) and 96h (dark grey). HCV RNA levels significantly increased between 48 and 72h post infection for all cell types tested. Cells were treated with anti-CD81 2s131 at 5μg/ml and HCV RNA copies assessed after 96h (black). Statistical comparisons were made using student's t-test, where * p

Techniques Used: Infection, Expressing, Real-time Polymerase Chain Reaction, Purification

13) Product Images from "Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier"

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

Journal: Gastroenterology

doi: 10.1053/j.gastro.2011.11.028

HCV RNA Load in Brain and Liver Tissue
Figure Legend Snippet: HCV RNA Load in Brain and Liver Tissue

Techniques Used:

HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were
Figure Legend Snippet: HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

Techniques Used: Expressing, Infection

14) Product Images from "Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier"

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

Journal: Gastroenterology

doi: 10.1053/j.gastro.2011.11.028

HCV RNA Load in Brain and Liver Tissue
Figure Legend Snippet: HCV RNA Load in Brain and Liver Tissue

Techniques Used:

HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were
Figure Legend Snippet: HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

Techniques Used: Expressing, Infection

15) Product Images from "Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier"

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

Journal: Gastroenterology

doi: 10.1053/j.gastro.2011.11.028

HCV RNA Load in Brain and Liver Tissue
Figure Legend Snippet: HCV RNA Load in Brain and Liver Tissue

Techniques Used:

HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were
Figure Legend Snippet: HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

Techniques Used: Expressing, Infection

16) Product Images from "Hepatitis C virus infection of neuroepithelioma cell lines"

Article Title: Hepatitis C virus infection of neuroepithelioma cell lines

Journal: Gastroenterology

doi: 10.1053/j.gastro.2010.06.008

HCVcc infection of hepatoma and neuroepithelioma cells HCVcc J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T cells for 12h. Unbound virus was removed by washing and the infection measured 72h later by quantifying focus forming units/ml (FFU/ml) (A). Representative images of NS5A + J6/JFH infected Huh-7.5, MC-IXC and SK-PN-DW cells denoting comparable levels of NS5A expression, where the scale bar represents 10μm. (B) Cellular RNA was extracted from uninfected (UF) and J6/JFH infected cells after 12h and 72h in the presence or absence of anti-NS3 VX-950 (10μg/ml) or Interferon-α (IFN-α, 100 IU/ml) and the level of HCV RNA copies assessed by qPCR. (C) J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T for 12h, unbound virus removed by washing and the cells lysed for RNA purification after 48h (white), 72h (light grey) and 96h (dark grey). HCV RNA levels significantly increased between 48 and 72h post infection for all cell types tested. Cells were treated with anti-CD81 2s131 at 5μg/ml and HCV RNA copies assessed after 96h (black). Statistical comparisons were made using student's t-test, where * p
Figure Legend Snippet: HCVcc infection of hepatoma and neuroepithelioma cells HCVcc J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T cells for 12h. Unbound virus was removed by washing and the infection measured 72h later by quantifying focus forming units/ml (FFU/ml) (A). Representative images of NS5A + J6/JFH infected Huh-7.5, MC-IXC and SK-PN-DW cells denoting comparable levels of NS5A expression, where the scale bar represents 10μm. (B) Cellular RNA was extracted from uninfected (UF) and J6/JFH infected cells after 12h and 72h in the presence or absence of anti-NS3 VX-950 (10μg/ml) or Interferon-α (IFN-α, 100 IU/ml) and the level of HCV RNA copies assessed by qPCR. (C) J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T for 12h, unbound virus removed by washing and the cells lysed for RNA purification after 48h (white), 72h (light grey) and 96h (dark grey). HCV RNA levels significantly increased between 48 and 72h post infection for all cell types tested. Cells were treated with anti-CD81 2s131 at 5μg/ml and HCV RNA copies assessed after 96h (black). Statistical comparisons were made using student's t-test, where * p

Techniques Used: Infection, Expressing, Real-time Polymerase Chain Reaction, Purification

17) Product Images from "Hepatitis C virus infection of neuroepithelioma cell lines"

Article Title: Hepatitis C virus infection of neuroepithelioma cell lines

Journal: Gastroenterology

doi: 10.1053/j.gastro.2010.06.008

HCVcc infection of hepatoma and neuroepithelioma cells HCVcc J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T cells for 12h. Unbound virus was removed by washing and the infection measured 72h later by quantifying focus forming units/ml (FFU/ml) (A). Representative images of NS5A + J6/JFH infected Huh-7.5, MC-IXC and SK-PN-DW cells denoting comparable levels of NS5A expression, where the scale bar represents 10μm. (B) Cellular RNA was extracted from uninfected (UF) and J6/JFH infected cells after 12h and 72h in the presence or absence of anti-NS3 VX-950 (10μg/ml) or Interferon-α (IFN-α, 100 IU/ml) and the level of HCV RNA copies assessed by qPCR. (C) J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T for 12h, unbound virus removed by washing and the cells lysed for RNA purification after 48h (white), 72h (light grey) and 96h (dark grey). HCV RNA levels significantly increased between 48 and 72h post infection for all cell types tested. Cells were treated with anti-CD81 2s131 at 5μg/ml and HCV RNA copies assessed after 96h (black). Statistical comparisons were made using student's t-test, where * p
Figure Legend Snippet: HCVcc infection of hepatoma and neuroepithelioma cells HCVcc J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T cells for 12h. Unbound virus was removed by washing and the infection measured 72h later by quantifying focus forming units/ml (FFU/ml) (A). Representative images of NS5A + J6/JFH infected Huh-7.5, MC-IXC and SK-PN-DW cells denoting comparable levels of NS5A expression, where the scale bar represents 10μm. (B) Cellular RNA was extracted from uninfected (UF) and J6/JFH infected cells after 12h and 72h in the presence or absence of anti-NS3 VX-950 (10μg/ml) or Interferon-α (IFN-α, 100 IU/ml) and the level of HCV RNA copies assessed by qPCR. (C) J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T for 12h, unbound virus removed by washing and the cells lysed for RNA purification after 48h (white), 72h (light grey) and 96h (dark grey). HCV RNA levels significantly increased between 48 and 72h post infection for all cell types tested. Cells were treated with anti-CD81 2s131 at 5μg/ml and HCV RNA copies assessed after 96h (black). Statistical comparisons were made using student's t-test, where * p

Techniques Used: Infection, Expressing, Real-time Polymerase Chain Reaction, Purification

18) Product Images from "Hepatitis C virus infection of neuroepithelioma cell lines"

Article Title: Hepatitis C virus infection of neuroepithelioma cell lines

Journal: Gastroenterology

doi: 10.1053/j.gastro.2010.06.008

HCVcc infection of hepatoma and neuroepithelioma cells HCVcc J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T cells for 12h. Unbound virus was removed by washing and the infection measured 72h later by quantifying focus forming units/ml (FFU/ml) (A). Representative images of NS5A + J6/JFH infected Huh-7.5, MC-IXC and SK-PN-DW cells denoting comparable levels of NS5A expression, where the scale bar represents 10μm. (B) Cellular RNA was extracted from uninfected (UF) and J6/JFH infected cells after 12h and 72h in the presence or absence of anti-NS3 VX-950 (10μg/ml) or Interferon-α (IFN-α, 100 IU/ml) and the level of HCV RNA copies assessed by qPCR. (C) J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T for 12h, unbound virus removed by washing and the cells lysed for RNA purification after 48h (white), 72h (light grey) and 96h (dark grey). HCV RNA levels significantly increased between 48 and 72h post infection for all cell types tested. Cells were treated with anti-CD81 2s131 at 5μg/ml and HCV RNA copies assessed after 96h (black). Statistical comparisons were made using student's t-test, where * p
Figure Legend Snippet: HCVcc infection of hepatoma and neuroepithelioma cells HCVcc J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T cells for 12h. Unbound virus was removed by washing and the infection measured 72h later by quantifying focus forming units/ml (FFU/ml) (A). Representative images of NS5A + J6/JFH infected Huh-7.5, MC-IXC and SK-PN-DW cells denoting comparable levels of NS5A expression, where the scale bar represents 10μm. (B) Cellular RNA was extracted from uninfected (UF) and J6/JFH infected cells after 12h and 72h in the presence or absence of anti-NS3 VX-950 (10μg/ml) or Interferon-α (IFN-α, 100 IU/ml) and the level of HCV RNA copies assessed by qPCR. (C) J6/JFH was allowed to infect Huh-7.5, MC-IXC, SK-PN-DW and 293T for 12h, unbound virus removed by washing and the cells lysed for RNA purification after 48h (white), 72h (light grey) and 96h (dark grey). HCV RNA levels significantly increased between 48 and 72h post infection for all cell types tested. Cells were treated with anti-CD81 2s131 at 5μg/ml and HCV RNA copies assessed after 96h (black). Statistical comparisons were made using student's t-test, where * p

Techniques Used: Infection, Expressing, Real-time Polymerase Chain Reaction, Purification

19) Product Images from "Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier"

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

Journal: Gastroenterology

doi: 10.1053/j.gastro.2011.11.028

HCV RNA Load in Brain and Liver Tissue
Figure Legend Snippet: HCV RNA Load in Brain and Liver Tissue

Techniques Used:

HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were
Figure Legend Snippet: HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

Techniques Used: Expressing, Infection

20) Product Images from "Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier"

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

Journal: Gastroenterology

doi: 10.1053/j.gastro.2011.11.028

HCV RNA Load in Brain and Liver Tissue
Figure Legend Snippet: HCV RNA Load in Brain and Liver Tissue

Techniques Used:

HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were
Figure Legend Snippet: HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

Techniques Used: Expressing, Infection

21) Product Images from "Hepatitis C virus infection of cholangiocarcinoma cell lines"

Article Title: Hepatitis C virus infection of cholangiocarcinoma cell lines

Journal: The Journal of General Virology

doi: 10.1099/vir.0.000090

HCVcc infection of cholangiocarcinoma cells. (a) Sk-ChA-1 cells were inoculated with HCVcc strain SA13/JFH (titre of 10 6 IU ml −1 based on Huh-7 cells) in the presence or absence of anti-HCV Ig (100 µg ml −1 ), anti-CD81 antibody (clone 2s131, 10 µg ml −1 ), protease inhibitor Telaprevir (1 µg ml −1 ) and interferon-α (10 IU ml −1 ). Data are presented as f.f.u. ml −1 calculated as NS5A antigen-expressing cells. (b) Sk-ChA-1, but not CC-LP-1, cells support HCV SA13/JFH RNA replication at comparable levels to PHH. HCV RNA levels were normalized to Huh-7 cells. (c) Cholangiocarcinoma cells do not express miR-122 but control miR-210 was detected. (d) Sk-ChA-1 cells secrete low levels of ApoE, whereas we failed to detect ApoE from CC-LP-1 cells, where the dotted line indicates the ELISA cut-off point. N = 3 independent experiments.
Figure Legend Snippet: HCVcc infection of cholangiocarcinoma cells. (a) Sk-ChA-1 cells were inoculated with HCVcc strain SA13/JFH (titre of 10 6 IU ml −1 based on Huh-7 cells) in the presence or absence of anti-HCV Ig (100 µg ml −1 ), anti-CD81 antibody (clone 2s131, 10 µg ml −1 ), protease inhibitor Telaprevir (1 µg ml −1 ) and interferon-α (10 IU ml −1 ). Data are presented as f.f.u. ml −1 calculated as NS5A antigen-expressing cells. (b) Sk-ChA-1, but not CC-LP-1, cells support HCV SA13/JFH RNA replication at comparable levels to PHH. HCV RNA levels were normalized to Huh-7 cells. (c) Cholangiocarcinoma cells do not express miR-122 but control miR-210 was detected. (d) Sk-ChA-1 cells secrete low levels of ApoE, whereas we failed to detect ApoE from CC-LP-1 cells, where the dotted line indicates the ELISA cut-off point. N = 3 independent experiments.

Techniques Used: Infection, Protease Inhibitor, Expressing, Enzyme-linked Immunosorbent Assay

Related Articles

TUNEL Assay:

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier
Article Snippet: .. Disruption of BBB integrity can lead to an infiltration of pathogens, cytokines, and immune cells to the brain parenchyma as reported for HIV-1 and West Nile viruses., hCMEC/D3 infection led to increased HCV RNA and antigen expression over time, with a cytopathic effect that associated with TUNEL staining and increased permeability to the paracellular permeability marker FD-70. .. These data support a model in which HCV infection may compromise BBB integrity, with implications for brain homeostasis in HCV infection.

Amplification:

Article Title: Hepatitis C virus infection of neuroepithelioma cell lines
Article Snippet: .. Purified cellular RNA samples (Qiagen) were amplified for HCV RNA (Primer Design Ltd) in a single tube RT-PCR in accordance with the manufacturer's guidelines (CellsDirect kit, Invitrogen) and fluorescence monitored in a MxPro 3000 real time PCR machine (Stratagene). .. In all reactions the housekeeping gene GAPDH was included as an internal endogenous control for amplification efficiency and for RNA quantification (primer-limited endogenous control, ABI).

Fluorescence:

Article Title: Hepatitis C virus infection of neuroepithelioma cell lines
Article Snippet: .. Purified cellular RNA samples (Qiagen) were amplified for HCV RNA (Primer Design Ltd) in a single tube RT-PCR in accordance with the manufacturer's guidelines (CellsDirect kit, Invitrogen) and fluorescence monitored in a MxPro 3000 real time PCR machine (Stratagene). .. In all reactions the housekeeping gene GAPDH was included as an internal endogenous control for amplification efficiency and for RNA quantification (primer-limited endogenous control, ABI).

Infection:

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier
Article Snippet: .. Disruption of BBB integrity can lead to an infiltration of pathogens, cytokines, and immune cells to the brain parenchyma as reported for HIV-1 and West Nile viruses., hCMEC/D3 infection led to increased HCV RNA and antigen expression over time, with a cytopathic effect that associated with TUNEL staining and increased permeability to the paracellular permeability marker FD-70. .. These data support a model in which HCV infection may compromise BBB integrity, with implications for brain homeostasis in HCV infection.

Purification:

Article Title: Hepatitis C virus infection of neuroepithelioma cell lines
Article Snippet: .. Purified cellular RNA samples (Qiagen) were amplified for HCV RNA (Primer Design Ltd) in a single tube RT-PCR in accordance with the manufacturer's guidelines (CellsDirect kit, Invitrogen) and fluorescence monitored in a MxPro 3000 real time PCR machine (Stratagene). .. In all reactions the housekeeping gene GAPDH was included as an internal endogenous control for amplification efficiency and for RNA quantification (primer-limited endogenous control, ABI).

Real-time Polymerase Chain Reaction:

Article Title: Hepatitis C virus infection of neuroepithelioma cell lines
Article Snippet: .. Purified cellular RNA samples (Qiagen) were amplified for HCV RNA (Primer Design Ltd) in a single tube RT-PCR in accordance with the manufacturer's guidelines (CellsDirect kit, Invitrogen) and fluorescence monitored in a MxPro 3000 real time PCR machine (Stratagene). .. In all reactions the housekeeping gene GAPDH was included as an internal endogenous control for amplification efficiency and for RNA quantification (primer-limited endogenous control, ABI).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Hepatitis C virus infection of neuroepithelioma cell lines
Article Snippet: .. Purified cellular RNA samples (Qiagen) were amplified for HCV RNA (Primer Design Ltd) in a single tube RT-PCR in accordance with the manufacturer's guidelines (CellsDirect kit, Invitrogen) and fluorescence monitored in a MxPro 3000 real time PCR machine (Stratagene). .. In all reactions the housekeeping gene GAPDH was included as an internal endogenous control for amplification efficiency and for RNA quantification (primer-limited endogenous control, ABI).

other:

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier
Article Snippet: HCV RNA was first detected in hCMEC/D3 cells at 24 hours and levels increased significantly by 48 hours, in parallel with the increasing number of NS5A-expressing cells ( ).

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier
Article Snippet: HCV RNA was detected in at least one brain region from 4 of 10 HCV-infected subjects, independent of HIV coinfection status.

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier
Article Snippet: It is worth noting that 6 HCV-infected subjects had no detectable viral RNA in the brain, despite having comparable levels of viral RNA in the liver and plasma ( ) to 4 subjects with no detectable HCV RNA in the brain.

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier
Article Snippet: HCV RNA was detected in brain tissue from 4 of 10 HCV-infected individuals, independent of human immunodeficiency virus (HIV) status ( ).

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier
Article Snippet: Quantification of HCV RNA from matched samples of white and grey matter, cerebellum, medulla, liver, and plasma revealed that, in clinical samples with detectable brain HCV, the viral load was 1000- to 10,000-fold lower in brain compared with liver from the same subject.

Expressing:

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier
Article Snippet: .. Disruption of BBB integrity can lead to an infiltration of pathogens, cytokines, and immune cells to the brain parenchyma as reported for HIV-1 and West Nile viruses., hCMEC/D3 infection led to increased HCV RNA and antigen expression over time, with a cytopathic effect that associated with TUNEL staining and increased permeability to the paracellular permeability marker FD-70. .. These data support a model in which HCV infection may compromise BBB integrity, with implications for brain homeostasis in HCV infection.

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier
Article Snippet: .. HCV RNA and NS5A expression in Huh-7 cells increased over time ( ). .. At 48 hours, the level of HCV RNA in Huh-7 cells was approximately 1000-fold higher than in hCMEC/D3 cells ( ).

Marker:

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier
Article Snippet: .. Disruption of BBB integrity can lead to an infiltration of pathogens, cytokines, and immune cells to the brain parenchyma as reported for HIV-1 and West Nile viruses., hCMEC/D3 infection led to increased HCV RNA and antigen expression over time, with a cytopathic effect that associated with TUNEL staining and increased permeability to the paracellular permeability marker FD-70. .. These data support a model in which HCV infection may compromise BBB integrity, with implications for brain homeostasis in HCV infection.

Staining:

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier
Article Snippet: .. Disruption of BBB integrity can lead to an infiltration of pathogens, cytokines, and immune cells to the brain parenchyma as reported for HIV-1 and West Nile viruses., hCMEC/D3 infection led to increased HCV RNA and antigen expression over time, with a cytopathic effect that associated with TUNEL staining and increased permeability to the paracellular permeability marker FD-70. .. These data support a model in which HCV infection may compromise BBB integrity, with implications for brain homeostasis in HCV infection.

Permeability:

Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier
Article Snippet: .. Disruption of BBB integrity can lead to an infiltration of pathogens, cytokines, and immune cells to the brain parenchyma as reported for HIV-1 and West Nile viruses., hCMEC/D3 infection led to increased HCV RNA and antigen expression over time, with a cytopathic effect that associated with TUNEL staining and increased permeability to the paracellular permeability marker FD-70. .. These data support a model in which HCV infection may compromise BBB integrity, with implications for brain homeostasis in HCV infection.

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    HCV RNA Load in Brain and Liver Tissue

    Journal: Gastroenterology

    Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

    doi: 10.1053/j.gastro.2011.11.028

    Figure Lengend Snippet: HCV RNA Load in Brain and Liver Tissue

    Article Snippet: Quantification of HCV RNA from matched samples of white and grey matter, cerebellum, medulla, liver, and plasma revealed that, in clinical samples with detectable brain HCV, the viral load was 1000- to 10,000-fold lower in brain compared with liver from the same subject.

    Techniques:

    HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

    Journal: Gastroenterology

    Article Title: Hepatitis C Virus Infects the Endothelial Cells of the Blood-Brain Barrier

    doi: 10.1053/j.gastro.2011.11.028

    Figure Lengend Snippet: HCV RNA and antigen expression in brain endothelial and hepatoma cells. hCMEC/D3 and Huh-7 cells were infected with HCVcc SA13/JFH for12hours, and unbound virus was removed by washing. ( A ) HCV RNA copies and ( B ) the frequency of NS5A-positive cells were

    Article Snippet: Quantification of HCV RNA from matched samples of white and grey matter, cerebellum, medulla, liver, and plasma revealed that, in clinical samples with detectable brain HCV, the viral load was 1000- to 10,000-fold lower in brain compared with liver from the same subject.

    Techniques: Expressing, Infection