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Shanghai Kehua hcv rna diagnostic kit
Hcv Rna Diagnostic Kit, supplied by Shanghai Kehua, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hcv rna diagnostic kit/product/Shanghai Kehua
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
hcv rna diagnostic kit - by Bioz Stars, 2020-05
92/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: The correlation of HCV RNA and HCV core antigen in different genotypes of HCV. The correlation of HCV RNA and HCV core antigen in different genotypes of HCV
Article Snippet: .. 2.2.1 HCV RNA test The HCV RNA load was measured by the real‐time PCR (CobasZ‐480 Real‐time Fluorescence Quantitative PCR Instrument, Roche Pharmaceuticals Ltd., Branchburg, NJ, USA) according to the specification of HCV RNA Diagnostic Kit (Shanghai Kehua Bio‐engineering Co., Ltd, Shanghai, China). ..

Diagnostic Assay:

Article Title: The correlation of HCV RNA and HCV core antigen in different genotypes of HCV. The correlation of HCV RNA and HCV core antigen in different genotypes of HCV
Article Snippet: .. 2.2.1 HCV RNA test The HCV RNA load was measured by the real‐time PCR (CobasZ‐480 Real‐time Fluorescence Quantitative PCR Instrument, Roche Pharmaceuticals Ltd., Branchburg, NJ, USA) according to the specification of HCV RNA Diagnostic Kit (Shanghai Kehua Bio‐engineering Co., Ltd, Shanghai, China). ..

Fluorescence:

Article Title: The correlation of HCV RNA and HCV core antigen in different genotypes of HCV. The correlation of HCV RNA and HCV core antigen in different genotypes of HCV
Article Snippet: .. 2.2.1 HCV RNA test The HCV RNA load was measured by the real‐time PCR (CobasZ‐480 Real‐time Fluorescence Quantitative PCR Instrument, Roche Pharmaceuticals Ltd., Branchburg, NJ, USA) according to the specification of HCV RNA Diagnostic Kit (Shanghai Kehua Bio‐engineering Co., Ltd, Shanghai, China). ..

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    Shanghai Kehua hcv rna pcr fluorescence quantitative diagnostic kit
    SZA has no inhibitory effect on viral replication, assembly and release. ( a ) Huh7 cells were infected with HCVcc of JFH-1 (MOI = 1) and treated by 20 μg/ml of SZA for 4 h during indicated time periods as shown in the left panel. At 48 h post-infection, infected cells were performed IF. Results are plotted as % of HCVcc infection compared to DMSO treated group in parallel. ( b ) Antiviral activity of SZA on replication. JFH-1 <t>RNA</t> was electroporated into Huh7 cells. At 4 h after electroporation, the cells were treated with the indicated concentrations of SZA for 4 h at 37 o C. Concentrations of telaprevir are shown in brackets of the figure. Results are shown as relative <t>HCV</t> RNA level compared to untreated group. ( c ) BB7 replicon cells were incubated with the indicated concentrations of SZA or telaprevir for 4 h. Viral RNA levels were determined by qPCR 48 h after treatment. Concentrations of telaprevir are in brackets of the figure. Results are plotted as relative HCV RNA level compared to DMSO treated group. ( d ) Antiviral activity of SZA on assembly and release. Huh7 cells were electroporated with JFH-1 RNA of HCV, followed by 4 h treatment of the indicated concentrations of SZA. At 48 h after electroporation, cells were subjected to three cycles of freeze and thaw to test the intracellular viral infectivity; supernatants of the electroporated cells were collected for the detection of extracellular viral infectivity. 100 μM naringenin was introduced as a positive control. Results are shown as relative HCV infectivity compared to DMSO treated group. Data shown as mean ± SD of three independent experiments.
    Hcv Rna Pcr Fluorescence Quantitative Diagnostic Kit, supplied by Shanghai Kehua, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcv rna pcr fluorescence quantitative diagnostic kit/product/Shanghai Kehua
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    hcv rna pcr fluorescence quantitative diagnostic kit - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    92
    Shanghai Kehua hcv rna diagnostic kit
    SZA has no inhibitory effect on viral replication, assembly and release. ( a ) Huh7 cells were infected with HCVcc of JFH-1 (MOI = 1) and treated by 20 μg/ml of SZA for 4 h during indicated time periods as shown in the left panel. At 48 h post-infection, infected cells were performed IF. Results are plotted as % of HCVcc infection compared to DMSO treated group in parallel. ( b ) Antiviral activity of SZA on replication. JFH-1 <t>RNA</t> was electroporated into Huh7 cells. At 4 h after electroporation, the cells were treated with the indicated concentrations of SZA for 4 h at 37 o C. Concentrations of telaprevir are shown in brackets of the figure. Results are shown as relative <t>HCV</t> RNA level compared to untreated group. ( c ) BB7 replicon cells were incubated with the indicated concentrations of SZA or telaprevir for 4 h. Viral RNA levels were determined by qPCR 48 h after treatment. Concentrations of telaprevir are in brackets of the figure. Results are plotted as relative HCV RNA level compared to DMSO treated group. ( d ) Antiviral activity of SZA on assembly and release. Huh7 cells were electroporated with JFH-1 RNA of HCV, followed by 4 h treatment of the indicated concentrations of SZA. At 48 h after electroporation, cells were subjected to three cycles of freeze and thaw to test the intracellular viral infectivity; supernatants of the electroporated cells were collected for the detection of extracellular viral infectivity. 100 μM naringenin was introduced as a positive control. Results are shown as relative HCV infectivity compared to DMSO treated group. Data shown as mean ± SD of three independent experiments.
    Hcv Rna Diagnostic Kit, supplied by Shanghai Kehua, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcv rna diagnostic kit/product/Shanghai Kehua
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    hcv rna diagnostic kit - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

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    SZA has no inhibitory effect on viral replication, assembly and release. ( a ) Huh7 cells were infected with HCVcc of JFH-1 (MOI = 1) and treated by 20 μg/ml of SZA for 4 h during indicated time periods as shown in the left panel. At 48 h post-infection, infected cells were performed IF. Results are plotted as % of HCVcc infection compared to DMSO treated group in parallel. ( b ) Antiviral activity of SZA on replication. JFH-1 RNA was electroporated into Huh7 cells. At 4 h after electroporation, the cells were treated with the indicated concentrations of SZA for 4 h at 37 o C. Concentrations of telaprevir are shown in brackets of the figure. Results are shown as relative HCV RNA level compared to untreated group. ( c ) BB7 replicon cells were incubated with the indicated concentrations of SZA or telaprevir for 4 h. Viral RNA levels were determined by qPCR 48 h after treatment. Concentrations of telaprevir are in brackets of the figure. Results are plotted as relative HCV RNA level compared to DMSO treated group. ( d ) Antiviral activity of SZA on assembly and release. Huh7 cells were electroporated with JFH-1 RNA of HCV, followed by 4 h treatment of the indicated concentrations of SZA. At 48 h after electroporation, cells were subjected to three cycles of freeze and thaw to test the intracellular viral infectivity; supernatants of the electroporated cells were collected for the detection of extracellular viral infectivity. 100 μM naringenin was introduced as a positive control. Results are shown as relative HCV infectivity compared to DMSO treated group. Data shown as mean ± SD of three independent experiments.

    Journal: Scientific Reports

    Article Title: A Schisandra-Derived Compound Schizandronic Acid Inhibits Entry of Pan-HCV Genotypes into Human Hepatocytes

    doi: 10.1038/srep27268

    Figure Lengend Snippet: SZA has no inhibitory effect on viral replication, assembly and release. ( a ) Huh7 cells were infected with HCVcc of JFH-1 (MOI = 1) and treated by 20 μg/ml of SZA for 4 h during indicated time periods as shown in the left panel. At 48 h post-infection, infected cells were performed IF. Results are plotted as % of HCVcc infection compared to DMSO treated group in parallel. ( b ) Antiviral activity of SZA on replication. JFH-1 RNA was electroporated into Huh7 cells. At 4 h after electroporation, the cells were treated with the indicated concentrations of SZA for 4 h at 37 o C. Concentrations of telaprevir are shown in brackets of the figure. Results are shown as relative HCV RNA level compared to untreated group. ( c ) BB7 replicon cells were incubated with the indicated concentrations of SZA or telaprevir for 4 h. Viral RNA levels were determined by qPCR 48 h after treatment. Concentrations of telaprevir are in brackets of the figure. Results are plotted as relative HCV RNA level compared to DMSO treated group. ( d ) Antiviral activity of SZA on assembly and release. Huh7 cells were electroporated with JFH-1 RNA of HCV, followed by 4 h treatment of the indicated concentrations of SZA. At 48 h after electroporation, cells were subjected to three cycles of freeze and thaw to test the intracellular viral infectivity; supernatants of the electroporated cells were collected for the detection of extracellular viral infectivity. 100 μM naringenin was introduced as a positive control. Results are shown as relative HCV infectivity compared to DMSO treated group. Data shown as mean ± SD of three independent experiments.

    Article Snippet: Total RNA of the serum was extracted using TRIzol LS reagent (Invitrogen, Carlsbad, CA), and HCV RNA level was detected by HCV RNA-PCR-Fluorescence Quantitative Diagnostic Kit (Kehua Bio-engineering Corporation, Shanghai, China).

    Techniques: Infection, Activity Assay, Electroporation, Incubation, Real-time Polymerase Chain Reaction, Positive Control

    SZA impairs virion-cell membrane fusion during HCV post-binding process. ( a ) The effect of SZA on viral internalization. Huh7 cells were incubated with HCVcc at 4 °C for 90 min for virus binding in the presence of SZA (20 μg/ml). Half of the cells were lysed to determine the bound viral particles, and the other cells were placed in a 37 o C incubator for 30 min to allow the internalization of viral particles followed by the trypsinization for 1 h on ice to remove the non-internalized virions. Quantities of the bound and the internalized viral particles were determined by RT-qPCR. Heparin was applied as a positive control for virus binding. Results are plotted as relative HCV RNA level compared to DMSO treated group during binding. ( b ) Huh7 cells were incubated with HCVcc of JFH-1 at 4 o C for 1.5 h to allow virus binding. The plate was then placed in a 37 o C incubator for synchronized virus entry. SZA (20 μg/ml), bafilomycin A1 (10 nM) or heparin (200 μg/ml) were added at different time points for 4 h treatment (red dotted lines in the right panel). At 48 h post-infection, HCVcc infection was analyzed by IF. Results are plotted as % of HCVcc infection compared to DMSO treated group of −1.5–2.5 h. Data shown as mean ± SD of three independent experiments in the above figures. (c) The effect of SZA on virion-cell fusion. HCV virion-cell fusion was measured by DiD fluorescence dequenching in Huh7 cells treated with SZA (20 μg/ml) in a kinetic mode. NH 4 Cl (10 mM) was introduced as a positive control. Results are plotted as relative fluorescence units after subtraction of the background (uninfected culture). Data shown as mean ± SD of triplicate wells from one independent experiment. **p

    Journal: Scientific Reports

    Article Title: A Schisandra-Derived Compound Schizandronic Acid Inhibits Entry of Pan-HCV Genotypes into Human Hepatocytes

    doi: 10.1038/srep27268

    Figure Lengend Snippet: SZA impairs virion-cell membrane fusion during HCV post-binding process. ( a ) The effect of SZA on viral internalization. Huh7 cells were incubated with HCVcc at 4 °C for 90 min for virus binding in the presence of SZA (20 μg/ml). Half of the cells were lysed to determine the bound viral particles, and the other cells were placed in a 37 o C incubator for 30 min to allow the internalization of viral particles followed by the trypsinization for 1 h on ice to remove the non-internalized virions. Quantities of the bound and the internalized viral particles were determined by RT-qPCR. Heparin was applied as a positive control for virus binding. Results are plotted as relative HCV RNA level compared to DMSO treated group during binding. ( b ) Huh7 cells were incubated with HCVcc of JFH-1 at 4 o C for 1.5 h to allow virus binding. The plate was then placed in a 37 o C incubator for synchronized virus entry. SZA (20 μg/ml), bafilomycin A1 (10 nM) or heparin (200 μg/ml) were added at different time points for 4 h treatment (red dotted lines in the right panel). At 48 h post-infection, HCVcc infection was analyzed by IF. Results are plotted as % of HCVcc infection compared to DMSO treated group of −1.5–2.5 h. Data shown as mean ± SD of three independent experiments in the above figures. (c) The effect of SZA on virion-cell fusion. HCV virion-cell fusion was measured by DiD fluorescence dequenching in Huh7 cells treated with SZA (20 μg/ml) in a kinetic mode. NH 4 Cl (10 mM) was introduced as a positive control. Results are plotted as relative fluorescence units after subtraction of the background (uninfected culture). Data shown as mean ± SD of triplicate wells from one independent experiment. **p

    Article Snippet: Total RNA of the serum was extracted using TRIzol LS reagent (Invitrogen, Carlsbad, CA), and HCV RNA level was detected by HCV RNA-PCR-Fluorescence Quantitative Diagnostic Kit (Kehua Bio-engineering Corporation, Shanghai, China).

    Techniques: Binding Assay, Incubation, Quantitative RT-PCR, Positive Control, Infection, Fluorescence

    SZA does not affect virus integrity, binding or entry factors expression. ( a ) Concentrated HCVcc of JFH-1 strain was incubated with SZA (20 μg/ml) or DMSO at 37 °C for 2 h before the compound was removed by filters. Iodixanol gradient ultracentrifugation was then performed. Each of the ten gradient fractions was weighed to calculate density (left panel). HCV RNA levels were determined by RT-qPCR (middle panel) and viral infectivity by reinfection of naïve Huh7 cells (right panel). ( b ) The effect of SZA on HCV binding. Huh7 cells were incubated with HCVcc at 4 o C for 2 h to facilitate virus binding. Indicated concentrations of either SZA or heparin was added. The quantity of bound viral particles was determined by RT-qPCR. Results are plotted as % of binding compared to untreated group. Data shown as mean ± SD of three independent experiments in the above figures. ( c ) The effect of SZA on the expression levels of cellular entry factors of HCV. Huh7 cells were incubated with SZA (50 μg/ml) for 4 h, and the expression levels of SRB1, CLDN1 and OCLN were determined by western blotting, ( d ) and CD81 by flow cytometry 24 h post-incubation.

    Journal: Scientific Reports

    Article Title: A Schisandra-Derived Compound Schizandronic Acid Inhibits Entry of Pan-HCV Genotypes into Human Hepatocytes

    doi: 10.1038/srep27268

    Figure Lengend Snippet: SZA does not affect virus integrity, binding or entry factors expression. ( a ) Concentrated HCVcc of JFH-1 strain was incubated with SZA (20 μg/ml) or DMSO at 37 °C for 2 h before the compound was removed by filters. Iodixanol gradient ultracentrifugation was then performed. Each of the ten gradient fractions was weighed to calculate density (left panel). HCV RNA levels were determined by RT-qPCR (middle panel) and viral infectivity by reinfection of naïve Huh7 cells (right panel). ( b ) The effect of SZA on HCV binding. Huh7 cells were incubated with HCVcc at 4 o C for 2 h to facilitate virus binding. Indicated concentrations of either SZA or heparin was added. The quantity of bound viral particles was determined by RT-qPCR. Results are plotted as % of binding compared to untreated group. Data shown as mean ± SD of three independent experiments in the above figures. ( c ) The effect of SZA on the expression levels of cellular entry factors of HCV. Huh7 cells were incubated with SZA (50 μg/ml) for 4 h, and the expression levels of SRB1, CLDN1 and OCLN were determined by western blotting, ( d ) and CD81 by flow cytometry 24 h post-incubation.

    Article Snippet: Total RNA of the serum was extracted using TRIzol LS reagent (Invitrogen, Carlsbad, CA), and HCV RNA level was detected by HCV RNA-PCR-Fluorescence Quantitative Diagnostic Kit (Kehua Bio-engineering Corporation, Shanghai, China).

    Techniques: Binding Assay, Expressing, Incubation, Quantitative RT-PCR, Infection, Western Blot, Flow Cytometry, Cytometry

    SZA diminishes HCV infection in transgenic mice. ( a ) ICR R+ mice were pretreated with DMSO (untreated) (n = 12) or SZA (n = 20) (5 mg per kg body weight per day) for 2 weeks before infection and 1 week after infection through intraperitoneal injection. Non-transgenic mice (n = 3) with ICR background was also injected with virus (blank). The blood samples were taken at different time points during the course of HCV infection (1/2/4/6 weeks post-infection). HCV RNA levels in different mice serum samples were tested using RT-qPCR. Data shown as mean ± SD of mice from different groups from one experiment. **p

    Journal: Scientific Reports

    Article Title: A Schisandra-Derived Compound Schizandronic Acid Inhibits Entry of Pan-HCV Genotypes into Human Hepatocytes

    doi: 10.1038/srep27268

    Figure Lengend Snippet: SZA diminishes HCV infection in transgenic mice. ( a ) ICR R+ mice were pretreated with DMSO (untreated) (n = 12) or SZA (n = 20) (5 mg per kg body weight per day) for 2 weeks before infection and 1 week after infection through intraperitoneal injection. Non-transgenic mice (n = 3) with ICR background was also injected with virus (blank). The blood samples were taken at different time points during the course of HCV infection (1/2/4/6 weeks post-infection). HCV RNA levels in different mice serum samples were tested using RT-qPCR. Data shown as mean ± SD of mice from different groups from one experiment. **p

    Article Snippet: Total RNA of the serum was extracted using TRIzol LS reagent (Invitrogen, Carlsbad, CA), and HCV RNA level was detected by HCV RNA-PCR-Fluorescence Quantitative Diagnostic Kit (Kehua Bio-engineering Corporation, Shanghai, China).

    Techniques: Infection, Transgenic Assay, Mouse Assay, Injection, Quantitative RT-PCR

    Gel electrophoresis of PCR and RT-PCR products . Lane M, DNA marker; Products amplified from pACYC-MS2-2V are represented in lanes 1-6: lane1, positive control; lane 2, RT-PCR product of RNA extracted from armored RNA; lanes 3-6, the 4 negative controls (H 2 O, H 2 O after extraction and RT, RNA extracted from armored RNAs without RT, and armored RNAs without extraction and RT); lanes 7-12 represent the same treatments, but amplified from pACYC-MS2-IC-2V.

    Journal: Virology Journal

    Article Title: Failure to detect Xenotropic murine leukaemia virus-related virus in Chinese patients with chronic fatigue syndrome

    doi: 10.1186/1743-422X-7-224

    Figure Lengend Snippet: Gel electrophoresis of PCR and RT-PCR products . Lane M, DNA marker; Products amplified from pACYC-MS2-2V are represented in lanes 1-6: lane1, positive control; lane 2, RT-PCR product of RNA extracted from armored RNA; lanes 3-6, the 4 negative controls (H 2 O, H 2 O after extraction and RT, RNA extracted from armored RNAs without RT, and armored RNAs without extraction and RT); lanes 7-12 represent the same treatments, but amplified from pACYC-MS2-IC-2V.

    Article Snippet: The purified armored RNAs were calibrated against the World Health Organization (WHO) Second International Standard for HCV RNA (National Institute for Biological Standards and Controls [NIBSC], code 96/798, UK), using an HCV RNA PCR fluorescence quantitative diagnostic kit (Shanghai, Kehua) [ ].

    Techniques: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Marker, Amplification, Positive Control

    Construction of CIC by overlapping PCR . During the first-round of PCR, 3 fragments (A, B and C: VP62 33-486 nt, VP62 486-1149, and the HCV 5'UTR) were amplified from plasmid VP62 (kindly provided by Lombardi) and pNCCL-HCV (constructed by our laboratory) using primers Gag-1S and Gag-1A, Gag-2S and Gag-2A, and HCV-S and HCV-A. In the first overlapping PCR, fragment D was amplified from fragments A and B using primers Gag-1S and Gag-2A; the internal probe-binding sequences were introduced into fragment D. Fragment CIC was obtained by a second overlapping PCR using primers Gag-1S and HCV-A to amplify from fragments D and C.

    Journal: Virology Journal

    Article Title: Failure to detect Xenotropic murine leukaemia virus-related virus in Chinese patients with chronic fatigue syndrome

    doi: 10.1186/1743-422X-7-224

    Figure Lengend Snippet: Construction of CIC by overlapping PCR . During the first-round of PCR, 3 fragments (A, B and C: VP62 33-486 nt, VP62 486-1149, and the HCV 5'UTR) were amplified from plasmid VP62 (kindly provided by Lombardi) and pNCCL-HCV (constructed by our laboratory) using primers Gag-1S and Gag-1A, Gag-2S and Gag-2A, and HCV-S and HCV-A. In the first overlapping PCR, fragment D was amplified from fragments A and B using primers Gag-1S and Gag-2A; the internal probe-binding sequences were introduced into fragment D. Fragment CIC was obtained by a second overlapping PCR using primers Gag-1S and HCV-A to amplify from fragments D and C.

    Article Snippet: The purified armored RNAs were calibrated against the World Health Organization (WHO) Second International Standard for HCV RNA (National Institute for Biological Standards and Controls [NIBSC], code 96/798, UK), using an HCV RNA PCR fluorescence quantitative diagnostic kit (Shanghai, Kehua) [ ].

    Techniques: Polymerase Chain Reaction, Amplification, Plasmid Preparation, Construct, Binding Assay