hcv 1a armored rna  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hcv 1a armored rna
    Association between IFN Response, <t>HCV</t> <t>RNA</t> Levels, and Effect on Host Gene Expression in HCV-Infected Mice Intrahepatic HCV RNA levels were determined by quantitative RT-PCR as described in Materials and Methods. Total number of genes showing increased expression in HCV-infected mice relative to donor-matched uninfected mice is shown in gray bars, while the percent of induced genes that are IFN regulated is shown using black triangles.
    Hcv 1a Armored Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcv 1a armored rna/product/Thermo Fisher
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    hcv 1a armored rna - by Bioz Stars, 2020-05
    85/100 stars

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    intrahepatic viral load

    Images

    1) Product Images from "Host-Specific Response to HCV Infection in the Chimeric SCID-beige/Alb-uPA Mouse Model: Role of the Innate Antiviral Immune Response"

    Article Title: Host-Specific Response to HCV Infection in the Chimeric SCID-beige/Alb-uPA Mouse Model: Role of the Innate Antiviral Immune Response

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.0020059

    Association between IFN Response, HCV RNA Levels, and Effect on Host Gene Expression in HCV-Infected Mice Intrahepatic HCV RNA levels were determined by quantitative RT-PCR as described in Materials and Methods. Total number of genes showing increased expression in HCV-infected mice relative to donor-matched uninfected mice is shown in gray bars, while the percent of induced genes that are IFN regulated is shown using black triangles.
    Figure Legend Snippet: Association between IFN Response, HCV RNA Levels, and Effect on Host Gene Expression in HCV-Infected Mice Intrahepatic HCV RNA levels were determined by quantitative RT-PCR as described in Materials and Methods. Total number of genes showing increased expression in HCV-infected mice relative to donor-matched uninfected mice is shown in gray bars, while the percent of induced genes that are IFN regulated is shown using black triangles.

    Techniques Used: Expressing, Infection, Mouse Assay, Quantitative RT-PCR

    2) Product Images from "Host-Specific Response to HCV Infection in the Chimeric SCID-beige/Alb-uPA Mouse Model: Role of the Innate Antiviral Immune Response"

    Article Title: Host-Specific Response to HCV Infection in the Chimeric SCID-beige/Alb-uPA Mouse Model: Role of the Innate Antiviral Immune Response

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.0020059

    Association between IFN Response, HCV RNA Levels, and Effect on Host Gene Expression in HCV-Infected Mice Intrahepatic HCV RNA levels were determined by quantitative RT-PCR as described in Materials and Methods. Total number of genes showing increased expression in HCV-infected mice relative to donor-matched uninfected mice is shown in gray bars, while the percent of induced genes that are IFN regulated is shown using black triangles.
    Figure Legend Snippet: Association between IFN Response, HCV RNA Levels, and Effect on Host Gene Expression in HCV-Infected Mice Intrahepatic HCV RNA levels were determined by quantitative RT-PCR as described in Materials and Methods. Total number of genes showing increased expression in HCV-infected mice relative to donor-matched uninfected mice is shown in gray bars, while the percent of induced genes that are IFN regulated is shown using black triangles.

    Techniques Used: Expressing, Infection, Mouse Assay, Quantitative RT-PCR

    Related Articles

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Genomic Analysis Reveals a Potential Role for Cell Cycle Perturbation in HCV-Mediated Apoptosis of Cultured Hepatocytes
    Article Snippet: .. Probes used for analysis (Applied Biosystems): Human genes: eukaryotic 18S rRNA (Catalogue No. Hs99999901_s1); ATF3 (catalogue No. Hs00910173_ml), MKi67 (catalogue No. Hs01032443_ml), MCM4 (catalogue No. Hs00381539_ml), MCM6 (catalogue No. Hs00195504_ml), TGIF1 (catalogue No. Hs00545014_ml), CAT (catalogue No. Hs00156308_ml), SMAD7 (catalogue No. Hs00998193_ml), GADD45A (catalogue No. Hs00169255_ml), GADD45B (catalogue No. Hs00169587_ml) Primer and probe sets for absolute quantification of intrahepatic viral load were designed based on sequences of HCV 1a armored RNA (Ambion Diagnostics, Austin, TX) using Primer Express (version 3). .. A standard curve was made from six serial dilutions of HCV 1a armored RNA (Ambion Diagnostics) with a known viral copy number.

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    Thermo Fisher in vitro synthetic biotin hcv 1a core rna g4
    NCL-R 3–4 C can stabilize target core <t>RNA</t> G4 and inhibit the trap by the corresponding ASO. ( A ) Schematic depiction of the inhibition of FRET. ( B ) Time course of the fluorescence intensity ( F – F 0 ) of a dual-labelled probe with increasing amounts of NCL-R 3–4 C. The final concentrations of <t>HCV</t> core RNA G4-dual and AS-HCV core RNA G4 were 200 nM and 2.0 mM, respectively. F – F 0 = (real time fluorescence intensity – initial fluorescence intensity)/initial fluorescence intensity. ( C ) G4 structure of HCV core RNA G4 evidenced by 1 H NMR. The final concentrations of HCV core RNA G4 and ASO were 0.5 and 0.1 mM, respectively.
    In Vitro Synthetic Biotin Hcv 1a Core Rna G4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in vitro synthetic biotin hcv 1a core rna g4/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    in vitro synthetic biotin hcv 1a core rna g4 - by Bioz Stars, 2020-05
    93/100 stars
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    NCL-R 3–4 C can stabilize target core RNA G4 and inhibit the trap by the corresponding ASO. ( A ) Schematic depiction of the inhibition of FRET. ( B ) Time course of the fluorescence intensity ( F – F 0 ) of a dual-labelled probe with increasing amounts of NCL-R 3–4 C. The final concentrations of HCV core RNA G4-dual and AS-HCV core RNA G4 were 200 nM and 2.0 mM, respectively. F – F 0 = (real time fluorescence intensity – initial fluorescence intensity)/initial fluorescence intensity. ( C ) G4 structure of HCV core RNA G4 evidenced by 1 H NMR. The final concentrations of HCV core RNA G4 and ASO were 0.5 and 0.1 mM, respectively.

    Journal: Nucleic Acids Research

    Article Title: Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication

    doi: 10.1093/nar/gky1177

    Figure Lengend Snippet: NCL-R 3–4 C can stabilize target core RNA G4 and inhibit the trap by the corresponding ASO. ( A ) Schematic depiction of the inhibition of FRET. ( B ) Time course of the fluorescence intensity ( F – F 0 ) of a dual-labelled probe with increasing amounts of NCL-R 3–4 C. The final concentrations of HCV core RNA G4-dual and AS-HCV core RNA G4 were 200 nM and 2.0 mM, respectively. F – F 0 = (real time fluorescence intensity – initial fluorescence intensity)/initial fluorescence intensity. ( C ) G4 structure of HCV core RNA G4 evidenced by 1 H NMR. The final concentrations of HCV core RNA G4 and ASO were 0.5 and 0.1 mM, respectively.

    Article Snippet: First, in vitro synthetic biotin-HCV 1a core RNA G4 (5′-biotin-GGGCUGCGGGUGGGCGGGA-3′) ( ) or G4-modified RNA (5′-biotin-GG A CUGCG U GUG A GCGGGA-3′, with the underlined letters representing modifications at G269A, G275U and G279A) (10 μg), was incubated in 10 mM Tris–HCl (pH 7.0) buffer containing 100 mM KCl to form the G4 structure and then incubated with streptavidin-agarose (Thermo Fisher, 20347) (20 μl) at 4°C for 2 h. It was then washed 3 times with PBS at 4°C.

    Techniques: Allele-specific Oligonucleotide, Inhibition, Fluorescence, Nuclear Magnetic Resonance

    The effects of G4 ligands on the colocalization of NCL and HCV core RNA G4 in Huh7.5.1 cells by confocal immunofluorescence analysis. Confocal immunofluorescence images with magnification 63×.

    Journal: Nucleic Acids Research

    Article Title: Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication

    doi: 10.1093/nar/gky1177

    Figure Lengend Snippet: The effects of G4 ligands on the colocalization of NCL and HCV core RNA G4 in Huh7.5.1 cells by confocal immunofluorescence analysis. Confocal immunofluorescence images with magnification 63×.

    Article Snippet: First, in vitro synthetic biotin-HCV 1a core RNA G4 (5′-biotin-GGGCUGCGGGUGGGCGGGA-3′) ( ) or G4-modified RNA (5′-biotin-GG A CUGCG U GUG A GCGGGA-3′, with the underlined letters representing modifications at G269A, G275U and G279A) (10 μg), was incubated in 10 mM Tris–HCl (pH 7.0) buffer containing 100 mM KCl to form the G4 structure and then incubated with streptavidin-agarose (Thermo Fisher, 20347) (20 μl) at 4°C for 2 h. It was then washed 3 times with PBS at 4°C.

    Techniques: Immunofluorescence

    Direct interaction and colocalization between HCV core RNA G4 and NCL in Huh7.5.1 cells. ( A ) RT-qPCR and Western blot analysis of HCV RNA and core protein expression. ( B ) G4 pull-down and Western blot. The fourth lane represents cell lysates directly used for western blot. β-actin was used as an internal control. ( C ) Colocalization of HCV core RNA G4 with NCL in Huh7.5.1 cells by confocal immunofluorescence. ( D ) Analysis of colocalization of wild-type HCV and modified G4 HCV with NCL in Huh7.5.1 cells by confocal immunofluorescence. Images in C and D with magnification 63×.

    Journal: Nucleic Acids Research

    Article Title: Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication

    doi: 10.1093/nar/gky1177

    Figure Lengend Snippet: Direct interaction and colocalization between HCV core RNA G4 and NCL in Huh7.5.1 cells. ( A ) RT-qPCR and Western blot analysis of HCV RNA and core protein expression. ( B ) G4 pull-down and Western blot. The fourth lane represents cell lysates directly used for western blot. β-actin was used as an internal control. ( C ) Colocalization of HCV core RNA G4 with NCL in Huh7.5.1 cells by confocal immunofluorescence. ( D ) Analysis of colocalization of wild-type HCV and modified G4 HCV with NCL in Huh7.5.1 cells by confocal immunofluorescence. Images in C and D with magnification 63×.

    Article Snippet: First, in vitro synthetic biotin-HCV 1a core RNA G4 (5′-biotin-GGGCUGCGGGUGGGCGGGA-3′) ( ) or G4-modified RNA (5′-biotin-GG A CUGCG U GUG A GCGGGA-3′, with the underlined letters representing modifications at G269A, G275U and G279A) (10 μg), was incubated in 10 mM Tris–HCl (pH 7.0) buffer containing 100 mM KCl to form the G4 structure and then incubated with streptavidin-agarose (Thermo Fisher, 20347) (20 μl) at 4°C for 2 h. It was then washed 3 times with PBS at 4°C.

    Techniques: Quantitative RT-PCR, Western Blot, Expressing, Immunofluorescence, Modification