hct116 fetal bovine serum  (Millipore)

 
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    Name:
    Fetal Bovine Serum
    Description:

    Catalog Number:
    F6765
    Price:
    None
    Applications:
    FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture. Charcoal stripped FBS is often used instead of FCS to minimize the level of androgen and other hormones provided in 10% serum supplemented media.
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    Structured Review

    Millipore hct116 fetal bovine serum
    Fetal Bovine Serum

    https://www.bioz.com/result/hct116 fetal bovine serum/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hct116 fetal bovine serum - by Bioz Stars, 2021-04
    98/100 stars

    Images

    1) Product Images from "Iron Chelators and Exogenic Photosensitizers. Synergy through Oxidative Stress Gene Expression"

    Article Title: Iron Chelators and Exogenic Photosensitizers. Synergy through Oxidative Stress Gene Expression

    Journal: Journal of Cancer

    doi: 10.7150/jca.17959

    Lipid peroxidation of the HCT116 cell line after incubation with TSCs, PSs and their combinations. Results are shown as the mean ± SD of three independent measurements. Data were analyzed using one-way ANOVA with Bonferroni's post-hoc test: *p
    Figure Legend Snippet: Lipid peroxidation of the HCT116 cell line after incubation with TSCs, PSs and their combinations. Results are shown as the mean ± SD of three independent measurements. Data were analyzed using one-way ANOVA with Bonferroni's post-hoc test: *p

    Techniques Used: Incubation

    mRNA expression of MnSOD and CAT in the HCT116 cell line after incubation with TSCs, PSs and their combinations. Results are shown as the mean ± SD of three independent measurements. Data were analyzed using the Student's t-test: *p
    Figure Legend Snippet: mRNA expression of MnSOD and CAT in the HCT116 cell line after incubation with TSCs, PSs and their combinations. Results are shown as the mean ± SD of three independent measurements. Data were analyzed using the Student's t-test: *p

    Techniques Used: Expressing, Incubation

    Cellular localization of TSCs; MS168 (a), Dp44mT (b), 3-AP (c) and PSs; chlorin c (d), Foscan (e) in the HCT116 cell line. First panel - fluorescence of the investigated compounds alone, middle panel - colocalization with mitochondria marker (MitoTracker® Orange for TSC's and Green for PS's), last panel - merge. Scale bar represents 50 μm.
    Figure Legend Snippet: Cellular localization of TSCs; MS168 (a), Dp44mT (b), 3-AP (c) and PSs; chlorin c (d), Foscan (e) in the HCT116 cell line. First panel - fluorescence of the investigated compounds alone, middle panel - colocalization with mitochondria marker (MitoTracker® Orange for TSC's and Green for PS's), last panel - merge. Scale bar represents 50 μm.

    Techniques Used: Fluorescence, Marker

    Related Articles

    Labeling:

    Article Title: A dual regulatory circuit consisting of S-adenosylmethionine decarboxylase protein and its reaction product controls expression of the paralogous activator prozyme in Trypanosoma brucei
    Article Snippet: Cycloheximide (Sigma) was used at 50 μg/mL [ ]. .. For 2 H7 -leucine labeling conditions, cell lines were plated into leucine-free HMI-19 medium (prepared with custom-ordered leucine-free IMDM (Invitrogen)) with 10% dialyzed FBS and supplemented with either 10 μM 2 H7 -leucine for SRM analysis or for growth studies supplemented with sterile-filtered leucine (Sigma) dissolved in ddH2 O to the desired concentrations. .. For methionine-limiting conditions, cell lines were cultured in methionine-free HMI-19 medium (prepared from custom-ordered methionine-free IMDM (Invitrogen)) supplemented with 10% F BS.

    Incubation:

    Article Title: Bovine Colostrum Increases Pore-Forming Claudin-2 Protein Expression but Paradoxically Not Ion Permeability Possibly by a Change of the Intestinal Cytokine Milieu
    Article Snippet: Measurement of the Permeability for Uncharged Macromolecules For studying TJ permeability to uncharged macromolecules, differentiated Caco-2 cells obtained 14 days post confluent (TER at least 300 Ω * cm2 ) were grown on cell culture inserts (6.4 mm, 0.3 cm2 , BD Falcon™, Germany) in 24 well plates. .. After 48 h culture with 5% BC or 5% FBS-enriched control media, 4 kDa fluorescein isothiocyanate-dextrane (4 kDa FITC-Dx; 2 mg/ml, Sigma, Germany) was applied to the apical well and cells were incubated for another 4 h. Unidirectional flux of 4 kDa FITC-Dx (excitation 495 nm, emission: 525) was measured in the basal well using a fluorescence plate reader (Tecan i control infinite 200, Germany). ..

    Article Title: Role of Bone Marrow-Derived Monocytes/Macrophages in the Repair of Mucosal Damage Caused by Irradiation and/or Anticancer Drugs in Colitis Model
    Article Snippet: For further analysis, BMCs were stained with 10 μ M CFDA-SE (Carboxyfluorescein diacetate, succinimidyl ester; Molecular probes; Invitrogen) for 15 min at 37°C. .. Preparation of Damaged Mucosa and Identification of Injected BMCs Dissected mucosa was incubated in calcium and magnesium-free HBSS (GIBCO) containing 2.5% heat-inactivated FBS and 1 mM DTT (Sigma-Aldrich) to remove mucus. .. The mucosa was then incubated in HBSS containing 1 mM EDTA (GIBCO) for 45 min at 37°C.

    Article Title: The Paramyxovirus Simian Virus 5 V Protein Slows Progression of the Cell Cycle
    Article Snippet: .. The cells were initially blocked in DMEM–10% FBS–P/S with 2 mM thymidine (Sigma-Aldrich) for 12 to 14 h, the cells were released from the first block by incubation with DMEM–10% FBS–P/S for 8 h, and then the cells were blocked by incubation with DMEM–10% FBS–P/S with 0.4 mM mimosine (Sigma-Aldrich) for 12 to 14 h. After synchronization the cells were either infected with SV5, mock infected, or transfected with expression plasmids. .. For the SV5 infections, HeLa T4 cells were infected with SV5 at an MOI of 3 or 10 PFU/cell.

    Fluorescence:

    Article Title: Bovine Colostrum Increases Pore-Forming Claudin-2 Protein Expression but Paradoxically Not Ion Permeability Possibly by a Change of the Intestinal Cytokine Milieu
    Article Snippet: Measurement of the Permeability for Uncharged Macromolecules For studying TJ permeability to uncharged macromolecules, differentiated Caco-2 cells obtained 14 days post confluent (TER at least 300 Ω * cm2 ) were grown on cell culture inserts (6.4 mm, 0.3 cm2 , BD Falcon™, Germany) in 24 well plates. .. After 48 h culture with 5% BC or 5% FBS-enriched control media, 4 kDa fluorescein isothiocyanate-dextrane (4 kDa FITC-Dx; 2 mg/ml, Sigma, Germany) was applied to the apical well and cells were incubated for another 4 h. Unidirectional flux of 4 kDa FITC-Dx (excitation 495 nm, emission: 525) was measured in the basal well using a fluorescence plate reader (Tecan i control infinite 200, Germany). ..

    Cell Culture:

    Article Title: Cytochalasin-B-Inducible Nanovesicle Mimics of Natural Extracellular Vesicles That Are Capable of Nucleic Acid Transfer
    Article Snippet: .. Intracellular FAM-ON delivery, mediated by EVs, MDNVs, or CINVs, was examined under three different conditions: (1) HEK 293 cells cultured in DMEM + 10% EV-depleted FBS; (2) HEK 293 cells cultured in Opti-MEM with 1 mM sodium pyruvate (Sigma, USA) and 5 mM HEPES (pH 7.3 ± 0.3, Sigma, USA); (3) HEK 293 cells cultured in Opti-MEM with 1 mM sodium pyruvate, 5 mM HEPES, and 10% EV-depleted FBS. .. Under each tested condition, 2–5 independent replicates were performed for HEK293 incubation with EVs, 2–4 independent replicates for MDNVs, and 2–3 independent replicates for CINVs.

    Article Title: Establishment of fast-growing serum-free immortalised cells from Chinese hamster lung tissues for biopharmaceutical production
    Article Snippet: .. Primary cell culture Primary cultures were performed by disseminating the lung tissue of female Chinese hamsters (Charles River Laboratories Japan, Inc., Kanagawa, Japan) that had been cut into 1-mm squares into IMDM (Sigma-Aldrich, St. Louis, MO, USA) containing 20% FBS (#172012, lot 12E183, Sigma-Aldrich). .. Cells were cultured in 100-mm dishes at 37 °C with 5% CO2.

    Injection:

    Article Title: Role of Bone Marrow-Derived Monocytes/Macrophages in the Repair of Mucosal Damage Caused by Irradiation and/or Anticancer Drugs in Colitis Model
    Article Snippet: For further analysis, BMCs were stained with 10 μ M CFDA-SE (Carboxyfluorescein diacetate, succinimidyl ester; Molecular probes; Invitrogen) for 15 min at 37°C. .. Preparation of Damaged Mucosa and Identification of Injected BMCs Dissected mucosa was incubated in calcium and magnesium-free HBSS (GIBCO) containing 2.5% heat-inactivated FBS and 1 mM DTT (Sigma-Aldrich) to remove mucus. .. The mucosa was then incubated in HBSS containing 1 mM EDTA (GIBCO) for 45 min at 37°C.

    Modification:

    Article Title: Amino Acids and mTOR Mediate Distinct Metabolic Checkpoints in Mammalian G1 Cell Cycle
    Article Snippet: Cells and cell culture conditions BJ hTERT, MCF7, MDA-MB-231, and Panc-1 cells were obtained from the American Tissue Type Culture Collection. .. All the cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Sigma). ..

    Blocking Assay:

    Article Title: The Paramyxovirus Simian Virus 5 V Protein Slows Progression of the Cell Cycle
    Article Snippet: .. The cells were initially blocked in DMEM–10% FBS–P/S with 2 mM thymidine (Sigma-Aldrich) for 12 to 14 h, the cells were released from the first block by incubation with DMEM–10% FBS–P/S for 8 h, and then the cells were blocked by incubation with DMEM–10% FBS–P/S with 0.4 mM mimosine (Sigma-Aldrich) for 12 to 14 h. After synchronization the cells were either infected with SV5, mock infected, or transfected with expression plasmids. .. For the SV5 infections, HeLa T4 cells were infected with SV5 at an MOI of 3 or 10 PFU/cell.

    Infection:

    Article Title: The Paramyxovirus Simian Virus 5 V Protein Slows Progression of the Cell Cycle
    Article Snippet: .. The cells were initially blocked in DMEM–10% FBS–P/S with 2 mM thymidine (Sigma-Aldrich) for 12 to 14 h, the cells were released from the first block by incubation with DMEM–10% FBS–P/S for 8 h, and then the cells were blocked by incubation with DMEM–10% FBS–P/S with 0.4 mM mimosine (Sigma-Aldrich) for 12 to 14 h. After synchronization the cells were either infected with SV5, mock infected, or transfected with expression plasmids. .. For the SV5 infections, HeLa T4 cells were infected with SV5 at an MOI of 3 or 10 PFU/cell.

    Transfection:

    Article Title: The Paramyxovirus Simian Virus 5 V Protein Slows Progression of the Cell Cycle
    Article Snippet: .. The cells were initially blocked in DMEM–10% FBS–P/S with 2 mM thymidine (Sigma-Aldrich) for 12 to 14 h, the cells were released from the first block by incubation with DMEM–10% FBS–P/S for 8 h, and then the cells were blocked by incubation with DMEM–10% FBS–P/S with 0.4 mM mimosine (Sigma-Aldrich) for 12 to 14 h. After synchronization the cells were either infected with SV5, mock infected, or transfected with expression plasmids. .. For the SV5 infections, HeLa T4 cells were infected with SV5 at an MOI of 3 or 10 PFU/cell.

    Expressing:

    Article Title: The Paramyxovirus Simian Virus 5 V Protein Slows Progression of the Cell Cycle
    Article Snippet: .. The cells were initially blocked in DMEM–10% FBS–P/S with 2 mM thymidine (Sigma-Aldrich) for 12 to 14 h, the cells were released from the first block by incubation with DMEM–10% FBS–P/S for 8 h, and then the cells were blocked by incubation with DMEM–10% FBS–P/S with 0.4 mM mimosine (Sigma-Aldrich) for 12 to 14 h. After synchronization the cells were either infected with SV5, mock infected, or transfected with expression plasmids. .. For the SV5 infections, HeLa T4 cells were infected with SV5 at an MOI of 3 or 10 PFU/cell.

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    • hct116 fetal bovine serum  (Millipore)
    98
    Millipore hct116 fetal bovine serum
    Lipid peroxidation of the <t>HCT116</t> cell line after incubation with TSCs, PSs and their combinations. Results are shown as the mean ± SD of three independent measurements. Data were analyzed using one-way ANOVA with Bonferroni's post-hoc test: *p
    Hct116 Fetal Bovine Serum, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hct116 fetal bovine serum/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hct116 fetal bovine serum - by Bioz Stars, 2021-04
    98/100 stars
    		  Buy from Supplier

    Image Search Results


    Lipid peroxidation of the HCT116 cell line after incubation with TSCs, PSs and their combinations. Results are shown as the mean ± SD of three independent measurements. Data were analyzed using one-way ANOVA with Bonferroni's post-hoc test: *p

    Journal: Journal of Cancer

    Article Title: Iron Chelators and Exogenic Photosensitizers. Synergy through Oxidative Stress Gene Expression

    doi: 10.7150/jca.17959

    Figure Lengend Snippet: Lipid peroxidation of the HCT116 cell line after incubation with TSCs, PSs and their combinations. Results are shown as the mean ± SD of three independent measurements. Data were analyzed using one-way ANOVA with Bonferroni's post-hoc test: *p

    Article Snippet: The cells were grown as monolayer cultures in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 12% (HCT116) Fetal Bovine Serum (Sigma) and standard antibiotics in 75 cm2 flasks (Nunc).

    Techniques: Incubation

    mRNA expression of MnSOD and CAT in the HCT116 cell line after incubation with TSCs, PSs and their combinations. Results are shown as the mean ± SD of three independent measurements. Data were analyzed using the Student's t-test: *p

    Journal: Journal of Cancer

    Article Title: Iron Chelators and Exogenic Photosensitizers. Synergy through Oxidative Stress Gene Expression

    doi: 10.7150/jca.17959

    Figure Lengend Snippet: mRNA expression of MnSOD and CAT in the HCT116 cell line after incubation with TSCs, PSs and their combinations. Results are shown as the mean ± SD of three independent measurements. Data were analyzed using the Student's t-test: *p

    Article Snippet: The cells were grown as monolayer cultures in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 12% (HCT116) Fetal Bovine Serum (Sigma) and standard antibiotics in 75 cm2 flasks (Nunc).

    Techniques: Expressing, Incubation

    Cellular localization of TSCs; MS168 (a), Dp44mT (b), 3-AP (c) and PSs; chlorin c (d), Foscan (e) in the HCT116 cell line. First panel - fluorescence of the investigated compounds alone, middle panel - colocalization with mitochondria marker (MitoTracker® Orange for TSC's and Green for PS's), last panel - merge. Scale bar represents 50 μm.

    Journal: Journal of Cancer

    Article Title: Iron Chelators and Exogenic Photosensitizers. Synergy through Oxidative Stress Gene Expression

    doi: 10.7150/jca.17959

    Figure Lengend Snippet: Cellular localization of TSCs; MS168 (a), Dp44mT (b), 3-AP (c) and PSs; chlorin c (d), Foscan (e) in the HCT116 cell line. First panel - fluorescence of the investigated compounds alone, middle panel - colocalization with mitochondria marker (MitoTracker® Orange for TSC's and Green for PS's), last panel - merge. Scale bar represents 50 μm.

    Article Snippet: The cells were grown as monolayer cultures in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 12% (HCT116) Fetal Bovine Serum (Sigma) and standard antibiotics in 75 cm2 flasks (Nunc).

    Techniques: Fluorescence, Marker