hcov oc43 viruses  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC hcov oc43 viruses
    Hcov Oc43 Viruses, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov oc43 viruses/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov oc43 viruses - by Bioz Stars, 2024-04
    86/100 stars

    Images

    hcov nl63  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC hcov nl63
    Infectivity of pseudoviruses bearing wild type (WT), monobasic furin cleavage site (mFCS) and deleted furin cleavage site (ΔFCS) spike proteins <t>of</t> <t>HCoV-NL63</t> (A), -229E (C), and -HKU1 (E) in 293T-ACE2, 293T-ACE2-APN, and 293T-TMPRSS2. Dotted line indicates background level (1 × 10 4 RLU/ml). Data are shown as means and standard deviations from three independent experiments. The tests for two-group comparison (mutant spike vs WT spike) were analyzed using GraphPad Prism software. Asterisk* denotes significance: ****: p ≤ 0.0001 . Western blotting of HCoV-NL63 (B), -229E (D), -HKU1 (F) and SARS-CoV-2 pseudoviruses (B, D and F) bearing indicated spike proteins. HCoV-NL63 (B), -229E (D), -HKU1 (F) and SARS-CoV-2 spike proteins were probed using anti-NL63-S1 mouse mAb (9D3F3), anti-229E-S1 mouse mAb (24C9F7), anti-HKU1-S1 rabbit polyclonal sera and rabbit pAb (Sino Biological, 40589-T62) respectively, Full length S0 and furin processed S1 subunits were indicated using arrows. HIV p24 probed using mouse hybridoma (183-H12-5) is shown for pseudovirus control.
    Hcov Nl63, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov nl63/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov nl63 - by Bioz Stars, 2024-04
    86/100 stars

    Images

    1) Product Images from "Characterization of spike processing and entry mechanisms of seasonal human coronaviruses NL63, 229E and HKU1"

    Article Title: Characterization of spike processing and entry mechanisms of seasonal human coronaviruses NL63, 229E and HKU1

    Journal: bioRxiv

    doi: 10.1101/2024.04.12.589332

    Infectivity of pseudoviruses bearing wild type (WT), monobasic furin cleavage site (mFCS) and deleted furin cleavage site (ΔFCS) spike proteins of HCoV-NL63 (A), -229E (C), and -HKU1 (E) in 293T-ACE2, 293T-ACE2-APN, and 293T-TMPRSS2. Dotted line indicates background level (1 × 10 4 RLU/ml). Data are shown as means and standard deviations from three independent experiments. The tests for two-group comparison (mutant spike vs WT spike) were analyzed using GraphPad Prism software. Asterisk* denotes significance: ****: p ≤ 0.0001 . Western blotting of HCoV-NL63 (B), -229E (D), -HKU1 (F) and SARS-CoV-2 pseudoviruses (B, D and F) bearing indicated spike proteins. HCoV-NL63 (B), -229E (D), -HKU1 (F) and SARS-CoV-2 spike proteins were probed using anti-NL63-S1 mouse mAb (9D3F3), anti-229E-S1 mouse mAb (24C9F7), anti-HKU1-S1 rabbit polyclonal sera and rabbit pAb (Sino Biological, 40589-T62) respectively, Full length S0 and furin processed S1 subunits were indicated using arrows. HIV p24 probed using mouse hybridoma (183-H12-5) is shown for pseudovirus control.
    Figure Legend Snippet: Infectivity of pseudoviruses bearing wild type (WT), monobasic furin cleavage site (mFCS) and deleted furin cleavage site (ΔFCS) spike proteins of HCoV-NL63 (A), -229E (C), and -HKU1 (E) in 293T-ACE2, 293T-ACE2-APN, and 293T-TMPRSS2. Dotted line indicates background level (1 × 10 4 RLU/ml). Data are shown as means and standard deviations from three independent experiments. The tests for two-group comparison (mutant spike vs WT spike) were analyzed using GraphPad Prism software. Asterisk* denotes significance: ****: p ≤ 0.0001 . Western blotting of HCoV-NL63 (B), -229E (D), -HKU1 (F) and SARS-CoV-2 pseudoviruses (B, D and F) bearing indicated spike proteins. HCoV-NL63 (B), -229E (D), -HKU1 (F) and SARS-CoV-2 spike proteins were probed using anti-NL63-S1 mouse mAb (9D3F3), anti-229E-S1 mouse mAb (24C9F7), anti-HKU1-S1 rabbit polyclonal sera and rabbit pAb (Sino Biological, 40589-T62) respectively, Full length S0 and furin processed S1 subunits were indicated using arrows. HIV p24 probed using mouse hybridoma (183-H12-5) is shown for pseudovirus control.

    Techniques Used: Infection, Comparison, Mutagenesis, Software, Western Blot

    (A) Chloroquine sensitivity of HCoV-NL63 and SARS-CoV-2 pseudoviruses in 293T-ACE2 and 293T-ACE2-TMPRSS2 cells. (B) Camostat mesylate sensitivity of HCoV-NL63 and SARS-CoV-2 pseudoviruses in 293T-ACE2 and 293T-ACE2-TMPRSS2 cells. (C) Chloroquine sensitivity of HCoV-229E pseudoviruses in 293T-ACE2-APN cells. (D) Ammonium chloride sensitivity of HCoV-229E pseudoviruses in 293T-ACE2-APN cells. (E) Chloroquine sensitivity of HCoV-HKU1 and SARS-CoV-2 pseudoviruses in 293T-TMPRSS2 and 293T-ACE2 cells. (F) Camostat mesylate sensitivity of HCoV-HKU1 and SARS-CoV-2 pseudoviruses in 293T-TMPRSS2 and 293T-ACE2-TMPRSS2 cells respectively. For all experiments, cells were pretreated with indicated inhibitor for 2 h prior to pseudovirus infection in the presence of inhibitor for a period of 48 h. X-axis indicates log concentration of inhibitor. Y axis indicates percentage inhibition compared to pseudovirus infection without inhibitor treatment. Results shown are representative of three independent experiments.
    Figure Legend Snippet: (A) Chloroquine sensitivity of HCoV-NL63 and SARS-CoV-2 pseudoviruses in 293T-ACE2 and 293T-ACE2-TMPRSS2 cells. (B) Camostat mesylate sensitivity of HCoV-NL63 and SARS-CoV-2 pseudoviruses in 293T-ACE2 and 293T-ACE2-TMPRSS2 cells. (C) Chloroquine sensitivity of HCoV-229E pseudoviruses in 293T-ACE2-APN cells. (D) Ammonium chloride sensitivity of HCoV-229E pseudoviruses in 293T-ACE2-APN cells. (E) Chloroquine sensitivity of HCoV-HKU1 and SARS-CoV-2 pseudoviruses in 293T-TMPRSS2 and 293T-ACE2 cells. (F) Camostat mesylate sensitivity of HCoV-HKU1 and SARS-CoV-2 pseudoviruses in 293T-TMPRSS2 and 293T-ACE2-TMPRSS2 cells respectively. For all experiments, cells were pretreated with indicated inhibitor for 2 h prior to pseudovirus infection in the presence of inhibitor for a period of 48 h. X-axis indicates log concentration of inhibitor. Y axis indicates percentage inhibition compared to pseudovirus infection without inhibitor treatment. Results shown are representative of three independent experiments.

    Techniques Used: Infection, Concentration Assay, Inhibition

    hcov oc43 strain atcc vr 759  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC hcov oc43 strain atcc vr 759
    Analysis of interferon (‐response) genes upon coronavirus infection and effects of IFN‐λ1 supplementation on viral load. (a) Volcano plots depicting shared gene expression profiles of bronchial epithelial cell cultures infected with SARS‐CoV, SARS‐CoV‐2 or MERS‐CoV for (a) 24, (b) 48 hpi or (c) 72 hpi in comparison to HCoV‐229E <t>and</t> <t>HCoV‐OC43;</t> n = 4. Red dots indicate significantly upregulated genes, and blue dots indicate significantly downregulated genes. (d) Heatmap of the overlap of significantly changed genes during 24, 48 and 72 hpi infection with SARS‐CoV‐2, SARS‐CoV, MERS‐CoV or HCoV‐229E or HCoV‐OC43 and mock as uninfected control. (e) Gene set variation analysis (GSVA) was performed on interferon response genes after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. As HCoV‐OC43 infection was conducted at 33°C, SARS‐CoV‐2 infection at 33°C was included as control (pink). (f) IFN‐λ1 and (g) IFN‐α1 gene expression after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. The error bar indicates the standard deviation. Significant differences in increased gene expression over time of infection ( P < 0.05) are shown by the * symbol. Two‐way ANOVA followed by an unprotected Fisher's least significance difference test was conducted to test for significance. (h) ALI‐PBEC cultures were pretreated with 5 ng mL −1 interferon lambda (IFN‐λ1) for 60 min and subsequently infected with SARS‐CoV‐2 for 3 days with IFN‐λ1 present in the basal medium. Extracellular viral RNA copies are depicted in log scale. Data in h were analysed with a paired t ‐test and are depicted as mean ± SEM. n = 4 independent donors.
    Hcov Oc43 Strain Atcc Vr 759, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov oc43 strain atcc vr 759/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov oc43 strain atcc vr 759 - by Bioz Stars, 2024-04
    86/100 stars

    Images

    1) Product Images from "SARS ‐ C o V ‐2‐infected human airway epithelial cell cultures uniquely lack interferon and immediate early gene responses caused by other coronaviruses"

    Article Title: SARS ‐ C o V ‐2‐infected human airway epithelial cell cultures uniquely lack interferon and immediate early gene responses caused by other coronaviruses

    Journal: Clinical & Translational Immunology

    doi: 10.1002/cti2.1503

    Analysis of interferon (‐response) genes upon coronavirus infection and effects of IFN‐λ1 supplementation on viral load. (a) Volcano plots depicting shared gene expression profiles of bronchial epithelial cell cultures infected with SARS‐CoV, SARS‐CoV‐2 or MERS‐CoV for (a) 24, (b) 48 hpi or (c) 72 hpi in comparison to HCoV‐229E and HCoV‐OC43; n = 4. Red dots indicate significantly upregulated genes, and blue dots indicate significantly downregulated genes. (d) Heatmap of the overlap of significantly changed genes during 24, 48 and 72 hpi infection with SARS‐CoV‐2, SARS‐CoV, MERS‐CoV or HCoV‐229E or HCoV‐OC43 and mock as uninfected control. (e) Gene set variation analysis (GSVA) was performed on interferon response genes after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. As HCoV‐OC43 infection was conducted at 33°C, SARS‐CoV‐2 infection at 33°C was included as control (pink). (f) IFN‐λ1 and (g) IFN‐α1 gene expression after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. The error bar indicates the standard deviation. Significant differences in increased gene expression over time of infection ( P < 0.05) are shown by the * symbol. Two‐way ANOVA followed by an unprotected Fisher's least significance difference test was conducted to test for significance. (h) ALI‐PBEC cultures were pretreated with 5 ng mL −1 interferon lambda (IFN‐λ1) for 60 min and subsequently infected with SARS‐CoV‐2 for 3 days with IFN‐λ1 present in the basal medium. Extracellular viral RNA copies are depicted in log scale. Data in h were analysed with a paired t ‐test and are depicted as mean ± SEM. n = 4 independent donors.
    Figure Legend Snippet: Analysis of interferon (‐response) genes upon coronavirus infection and effects of IFN‐λ1 supplementation on viral load. (a) Volcano plots depicting shared gene expression profiles of bronchial epithelial cell cultures infected with SARS‐CoV, SARS‐CoV‐2 or MERS‐CoV for (a) 24, (b) 48 hpi or (c) 72 hpi in comparison to HCoV‐229E and HCoV‐OC43; n = 4. Red dots indicate significantly upregulated genes, and blue dots indicate significantly downregulated genes. (d) Heatmap of the overlap of significantly changed genes during 24, 48 and 72 hpi infection with SARS‐CoV‐2, SARS‐CoV, MERS‐CoV or HCoV‐229E or HCoV‐OC43 and mock as uninfected control. (e) Gene set variation analysis (GSVA) was performed on interferon response genes after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. As HCoV‐OC43 infection was conducted at 33°C, SARS‐CoV‐2 infection at 33°C was included as control (pink). (f) IFN‐λ1 and (g) IFN‐α1 gene expression after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. The error bar indicates the standard deviation. Significant differences in increased gene expression over time of infection ( P < 0.05) are shown by the * symbol. Two‐way ANOVA followed by an unprotected Fisher's least significance difference test was conducted to test for significance. (h) ALI‐PBEC cultures were pretreated with 5 ng mL −1 interferon lambda (IFN‐λ1) for 60 min and subsequently infected with SARS‐CoV‐2 for 3 days with IFN‐λ1 present in the basal medium. Extracellular viral RNA copies are depicted in log scale. Data in h were analysed with a paired t ‐test and are depicted as mean ± SEM. n = 4 independent donors.

    Techniques Used: Infection, Expressing, Comparison, Standard Deviation

    hcov oc43  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC hcov oc43
    Hcov Oc43, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov oc43/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov oc43 - by Bioz Stars, 2024-04
    86/100 stars

    Images

    hcov oc43  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC hcov oc43
    Hcov Oc43, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov oc43/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov oc43 - by Bioz Stars, 2024-04
    86/100 stars

    Images

    hcov 229e strain vr 740  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC hcov 229e strain vr 740
    Hcov 229e Strain Vr 740, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov 229e strain vr 740/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov 229e strain vr 740 - by Bioz Stars, 2024-04
    86/100 stars

    Images

    hcov oc43 strain vr 1558  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC hcov oc43 strain vr 1558
    Hcov Oc43 Strain Vr 1558, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov oc43 strain vr 1558/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov oc43 strain vr 1558 - by Bioz Stars, 2024-04
    86/100 stars

    Images

    hcov 229e strain vr 740  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC hcov 229e strain vr 740
    Hcov 229e Strain Vr 740, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov 229e strain vr 740/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov 229e strain vr 740 - by Bioz Stars, 2024-04
    86/100 stars

    Images

    hcov oc43 strain vr 1558  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC hcov oc43 strain vr 1558
    In vivo antiviral efficacy studies of compounds 14a and 14b . a Schematic diagram of in vivo antiviral potency study process of compounds 14a and 14b in K18-hACE2 mice challenged with SARS-CoV-2 XBB 1.16 variant. b Viral RNA copy numbers in the lung tissues of SARS-CoV-2 XBB 1.16 variant infected mice after two doses treatment with vehicle, compounds 14a and 14b . c Body weight changes <t>of</t> <t>HCoV-OC43-infected</t> mice after vehicle or compound 14b treatment. d Survival rate of HCoV-OC43-infected mice after vehicle or compound 14b treatment. Results are shown as mean ± SEM. ** P < 0.01, by two-tailed, unpaired t test
    Hcov Oc43 Strain Vr 1558, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov oc43 strain vr 1558/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov oc43 strain vr 1558 - by Bioz Stars, 2024-04
    86/100 stars

    Images

    1) Product Images from "Structure-based design of pan-coronavirus inhibitors targeting host cathepsin L and calpain-1"

    Article Title: Structure-based design of pan-coronavirus inhibitors targeting host cathepsin L and calpain-1

    Journal: Signal Transduction and Targeted Therapy

    doi: 10.1038/s41392-024-01758-8

    In vivo antiviral efficacy studies of compounds 14a and 14b . a Schematic diagram of in vivo antiviral potency study process of compounds 14a and 14b in K18-hACE2 mice challenged with SARS-CoV-2 XBB 1.16 variant. b Viral RNA copy numbers in the lung tissues of SARS-CoV-2 XBB 1.16 variant infected mice after two doses treatment with vehicle, compounds 14a and 14b . c Body weight changes of HCoV-OC43-infected mice after vehicle or compound 14b treatment. d Survival rate of HCoV-OC43-infected mice after vehicle or compound 14b treatment. Results are shown as mean ± SEM. ** P < 0.01, by two-tailed, unpaired t test
    Figure Legend Snippet: In vivo antiviral efficacy studies of compounds 14a and 14b . a Schematic diagram of in vivo antiviral potency study process of compounds 14a and 14b in K18-hACE2 mice challenged with SARS-CoV-2 XBB 1.16 variant. b Viral RNA copy numbers in the lung tissues of SARS-CoV-2 XBB 1.16 variant infected mice after two doses treatment with vehicle, compounds 14a and 14b . c Body weight changes of HCoV-OC43-infected mice after vehicle or compound 14b treatment. d Survival rate of HCoV-OC43-infected mice after vehicle or compound 14b treatment. Results are shown as mean ± SEM. ** P < 0.01, by two-tailed, unpaired t test

    Techniques Used: In Vivo, Variant Assay, Infection, Two Tailed Test

    hcov oc43  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC hcov oc43
    (A) Change <t>in</t> <t>HCoV-OC43</t> infectivity (PFU mL -1 ) in BHK-21 cell cultures along time in logarithm scales. The solid line represents the fit to the biphasic linear model shown in Eq. 1. (B) HCoV-OC43 infectivity decay in complete fresh media. The solid line represents the fit to a simple linear model. In both panels, the three experimental replicates are represented by different symbols.
    Hcov Oc43, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov oc43/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov oc43 - by Bioz Stars, 2024-04
    86/100 stars

    Images

    1) Product Images from "Decay of HCoV-OC43 infectivity is lower in cell debris-containing media than in fresh culture media"

    Article Title: Decay of HCoV-OC43 infectivity is lower in cell debris-containing media than in fresh culture media

    Journal: microPublication Biology

    doi: 10.17912/micropub.biology.001092

    (A) Change in HCoV-OC43 infectivity (PFU mL -1 ) in BHK-21 cell cultures along time in logarithm scales. The solid line represents the fit to the biphasic linear model shown in Eq. 1. (B) HCoV-OC43 infectivity decay in complete fresh media. The solid line represents the fit to a simple linear model. In both panels, the three experimental replicates are represented by different symbols.
    Figure Legend Snippet: (A) Change in HCoV-OC43 infectivity (PFU mL -1 ) in BHK-21 cell cultures along time in logarithm scales. The solid line represents the fit to the biphasic linear model shown in Eq. 1. (B) HCoV-OC43 infectivity decay in complete fresh media. The solid line represents the fit to a simple linear model. In both panels, the three experimental replicates are represented by different symbols.

    Techniques Used: Infection

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    ATCC hcov oc43 viruses
    Hcov Oc43 Viruses, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov oc43 viruses/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov oc43 viruses - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    86
    ATCC hcov nl63
    Infectivity of pseudoviruses bearing wild type (WT), monobasic furin cleavage site (mFCS) and deleted furin cleavage site (ΔFCS) spike proteins <t>of</t> <t>HCoV-NL63</t> (A), -229E (C), and -HKU1 (E) in 293T-ACE2, 293T-ACE2-APN, and 293T-TMPRSS2. Dotted line indicates background level (1 × 10 4 RLU/ml). Data are shown as means and standard deviations from three independent experiments. The tests for two-group comparison (mutant spike vs WT spike) were analyzed using GraphPad Prism software. Asterisk* denotes significance: ****: p ≤ 0.0001 . Western blotting of HCoV-NL63 (B), -229E (D), -HKU1 (F) and SARS-CoV-2 pseudoviruses (B, D and F) bearing indicated spike proteins. HCoV-NL63 (B), -229E (D), -HKU1 (F) and SARS-CoV-2 spike proteins were probed using anti-NL63-S1 mouse mAb (9D3F3), anti-229E-S1 mouse mAb (24C9F7), anti-HKU1-S1 rabbit polyclonal sera and rabbit pAb (Sino Biological, 40589-T62) respectively, Full length S0 and furin processed S1 subunits were indicated using arrows. HIV p24 probed using mouse hybridoma (183-H12-5) is shown for pseudovirus control.
    Hcov Nl63, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov nl63/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov nl63 - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    86
    ATCC hcov oc43 strain atcc vr 759
    Analysis of interferon (‐response) genes upon coronavirus infection and effects of IFN‐λ1 supplementation on viral load. (a) Volcano plots depicting shared gene expression profiles of bronchial epithelial cell cultures infected with SARS‐CoV, SARS‐CoV‐2 or MERS‐CoV for (a) 24, (b) 48 hpi or (c) 72 hpi in comparison to HCoV‐229E <t>and</t> <t>HCoV‐OC43;</t> n = 4. Red dots indicate significantly upregulated genes, and blue dots indicate significantly downregulated genes. (d) Heatmap of the overlap of significantly changed genes during 24, 48 and 72 hpi infection with SARS‐CoV‐2, SARS‐CoV, MERS‐CoV or HCoV‐229E or HCoV‐OC43 and mock as uninfected control. (e) Gene set variation analysis (GSVA) was performed on interferon response genes after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. As HCoV‐OC43 infection was conducted at 33°C, SARS‐CoV‐2 infection at 33°C was included as control (pink). (f) IFN‐λ1 and (g) IFN‐α1 gene expression after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. The error bar indicates the standard deviation. Significant differences in increased gene expression over time of infection ( P < 0.05) are shown by the * symbol. Two‐way ANOVA followed by an unprotected Fisher's least significance difference test was conducted to test for significance. (h) ALI‐PBEC cultures were pretreated with 5 ng mL −1 interferon lambda (IFN‐λ1) for 60 min and subsequently infected with SARS‐CoV‐2 for 3 days with IFN‐λ1 present in the basal medium. Extracellular viral RNA copies are depicted in log scale. Data in h were analysed with a paired t ‐test and are depicted as mean ± SEM. n = 4 independent donors.
    Hcov Oc43 Strain Atcc Vr 759, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov oc43 strain atcc vr 759/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov oc43 strain atcc vr 759 - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    86
    ATCC hcov oc43
    Analysis of interferon (‐response) genes upon coronavirus infection and effects of IFN‐λ1 supplementation on viral load. (a) Volcano plots depicting shared gene expression profiles of bronchial epithelial cell cultures infected with SARS‐CoV, SARS‐CoV‐2 or MERS‐CoV for (a) 24, (b) 48 hpi or (c) 72 hpi in comparison to HCoV‐229E <t>and</t> <t>HCoV‐OC43;</t> n = 4. Red dots indicate significantly upregulated genes, and blue dots indicate significantly downregulated genes. (d) Heatmap of the overlap of significantly changed genes during 24, 48 and 72 hpi infection with SARS‐CoV‐2, SARS‐CoV, MERS‐CoV or HCoV‐229E or HCoV‐OC43 and mock as uninfected control. (e) Gene set variation analysis (GSVA) was performed on interferon response genes after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. As HCoV‐OC43 infection was conducted at 33°C, SARS‐CoV‐2 infection at 33°C was included as control (pink). (f) IFN‐λ1 and (g) IFN‐α1 gene expression after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. The error bar indicates the standard deviation. Significant differences in increased gene expression over time of infection ( P < 0.05) are shown by the * symbol. Two‐way ANOVA followed by an unprotected Fisher's least significance difference test was conducted to test for significance. (h) ALI‐PBEC cultures were pretreated with 5 ng mL −1 interferon lambda (IFN‐λ1) for 60 min and subsequently infected with SARS‐CoV‐2 for 3 days with IFN‐λ1 present in the basal medium. Extracellular viral RNA copies are depicted in log scale. Data in h were analysed with a paired t ‐test and are depicted as mean ± SEM. n = 4 independent donors.
    Hcov Oc43, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov oc43/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov oc43 - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    86
    ATCC hcov 229e strain vr 740
    Analysis of interferon (‐response) genes upon coronavirus infection and effects of IFN‐λ1 supplementation on viral load. (a) Volcano plots depicting shared gene expression profiles of bronchial epithelial cell cultures infected with SARS‐CoV, SARS‐CoV‐2 or MERS‐CoV for (a) 24, (b) 48 hpi or (c) 72 hpi in comparison to HCoV‐229E <t>and</t> <t>HCoV‐OC43;</t> n = 4. Red dots indicate significantly upregulated genes, and blue dots indicate significantly downregulated genes. (d) Heatmap of the overlap of significantly changed genes during 24, 48 and 72 hpi infection with SARS‐CoV‐2, SARS‐CoV, MERS‐CoV or HCoV‐229E or HCoV‐OC43 and mock as uninfected control. (e) Gene set variation analysis (GSVA) was performed on interferon response genes after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. As HCoV‐OC43 infection was conducted at 33°C, SARS‐CoV‐2 infection at 33°C was included as control (pink). (f) IFN‐λ1 and (g) IFN‐α1 gene expression after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. The error bar indicates the standard deviation. Significant differences in increased gene expression over time of infection ( P < 0.05) are shown by the * symbol. Two‐way ANOVA followed by an unprotected Fisher's least significance difference test was conducted to test for significance. (h) ALI‐PBEC cultures were pretreated with 5 ng mL −1 interferon lambda (IFN‐λ1) for 60 min and subsequently infected with SARS‐CoV‐2 for 3 days with IFN‐λ1 present in the basal medium. Extracellular viral RNA copies are depicted in log scale. Data in h were analysed with a paired t ‐test and are depicted as mean ± SEM. n = 4 independent donors.
    Hcov 229e Strain Vr 740, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov 229e strain vr 740/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov 229e strain vr 740 - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    86
    ATCC hcov oc43 strain vr 1558
    Analysis of interferon (‐response) genes upon coronavirus infection and effects of IFN‐λ1 supplementation on viral load. (a) Volcano plots depicting shared gene expression profiles of bronchial epithelial cell cultures infected with SARS‐CoV, SARS‐CoV‐2 or MERS‐CoV for (a) 24, (b) 48 hpi or (c) 72 hpi in comparison to HCoV‐229E <t>and</t> <t>HCoV‐OC43;</t> n = 4. Red dots indicate significantly upregulated genes, and blue dots indicate significantly downregulated genes. (d) Heatmap of the overlap of significantly changed genes during 24, 48 and 72 hpi infection with SARS‐CoV‐2, SARS‐CoV, MERS‐CoV or HCoV‐229E or HCoV‐OC43 and mock as uninfected control. (e) Gene set variation analysis (GSVA) was performed on interferon response genes after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. As HCoV‐OC43 infection was conducted at 33°C, SARS‐CoV‐2 infection at 33°C was included as control (pink). (f) IFN‐λ1 and (g) IFN‐α1 gene expression after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. The error bar indicates the standard deviation. Significant differences in increased gene expression over time of infection ( P < 0.05) are shown by the * symbol. Two‐way ANOVA followed by an unprotected Fisher's least significance difference test was conducted to test for significance. (h) ALI‐PBEC cultures were pretreated with 5 ng mL −1 interferon lambda (IFN‐λ1) for 60 min and subsequently infected with SARS‐CoV‐2 for 3 days with IFN‐λ1 present in the basal medium. Extracellular viral RNA copies are depicted in log scale. Data in h were analysed with a paired t ‐test and are depicted as mean ± SEM. n = 4 independent donors.
    Hcov Oc43 Strain Vr 1558, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcov oc43 strain vr 1558/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hcov oc43 strain vr 1558 - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Infectivity of pseudoviruses bearing wild type (WT), monobasic furin cleavage site (mFCS) and deleted furin cleavage site (ΔFCS) spike proteins of HCoV-NL63 (A), -229E (C), and -HKU1 (E) in 293T-ACE2, 293T-ACE2-APN, and 293T-TMPRSS2. Dotted line indicates background level (1 × 10 4 RLU/ml). Data are shown as means and standard deviations from three independent experiments. The tests for two-group comparison (mutant spike vs WT spike) were analyzed using GraphPad Prism software. Asterisk* denotes significance: ****: p ≤ 0.0001 . Western blotting of HCoV-NL63 (B), -229E (D), -HKU1 (F) and SARS-CoV-2 pseudoviruses (B, D and F) bearing indicated spike proteins. HCoV-NL63 (B), -229E (D), -HKU1 (F) and SARS-CoV-2 spike proteins were probed using anti-NL63-S1 mouse mAb (9D3F3), anti-229E-S1 mouse mAb (24C9F7), anti-HKU1-S1 rabbit polyclonal sera and rabbit pAb (Sino Biological, 40589-T62) respectively, Full length S0 and furin processed S1 subunits were indicated using arrows. HIV p24 probed using mouse hybridoma (183-H12-5) is shown for pseudovirus control.

    Journal: bioRxiv

    Article Title: Characterization of spike processing and entry mechanisms of seasonal human coronaviruses NL63, 229E and HKU1

    doi: 10.1101/2024.04.12.589332

    Figure Lengend Snippet: Infectivity of pseudoviruses bearing wild type (WT), monobasic furin cleavage site (mFCS) and deleted furin cleavage site (ΔFCS) spike proteins of HCoV-NL63 (A), -229E (C), and -HKU1 (E) in 293T-ACE2, 293T-ACE2-APN, and 293T-TMPRSS2. Dotted line indicates background level (1 × 10 4 RLU/ml). Data are shown as means and standard deviations from three independent experiments. The tests for two-group comparison (mutant spike vs WT spike) were analyzed using GraphPad Prism software. Asterisk* denotes significance: ****: p ≤ 0.0001 . Western blotting of HCoV-NL63 (B), -229E (D), -HKU1 (F) and SARS-CoV-2 pseudoviruses (B, D and F) bearing indicated spike proteins. HCoV-NL63 (B), -229E (D), -HKU1 (F) and SARS-CoV-2 spike proteins were probed using anti-NL63-S1 mouse mAb (9D3F3), anti-229E-S1 mouse mAb (24C9F7), anti-HKU1-S1 rabbit polyclonal sera and rabbit pAb (Sino Biological, 40589-T62) respectively, Full length S0 and furin processed S1 subunits were indicated using arrows. HIV p24 probed using mouse hybridoma (183-H12-5) is shown for pseudovirus control.

    Article Snippet: Pseudoviruses bearing the desired spike glycoprotein of HCoV-NL63, -229E, -HKU1 and SARS-CoV-2 and carrying a firefly luciferase (FLuc) reporter gene were produced by cotransfecting HEK-293T (ATCC, Cat no: CRL-11268) cells with 5 μg of pCMVΔR8.2, 5 μg of pHR’CMVLuc and 0.5 μg of pVRC spike or its corresponding mutant expression plasmids.

    Techniques: Infection, Comparison, Mutagenesis, Software, Western Blot

    (A) Chloroquine sensitivity of HCoV-NL63 and SARS-CoV-2 pseudoviruses in 293T-ACE2 and 293T-ACE2-TMPRSS2 cells. (B) Camostat mesylate sensitivity of HCoV-NL63 and SARS-CoV-2 pseudoviruses in 293T-ACE2 and 293T-ACE2-TMPRSS2 cells. (C) Chloroquine sensitivity of HCoV-229E pseudoviruses in 293T-ACE2-APN cells. (D) Ammonium chloride sensitivity of HCoV-229E pseudoviruses in 293T-ACE2-APN cells. (E) Chloroquine sensitivity of HCoV-HKU1 and SARS-CoV-2 pseudoviruses in 293T-TMPRSS2 and 293T-ACE2 cells. (F) Camostat mesylate sensitivity of HCoV-HKU1 and SARS-CoV-2 pseudoviruses in 293T-TMPRSS2 and 293T-ACE2-TMPRSS2 cells respectively. For all experiments, cells were pretreated with indicated inhibitor for 2 h prior to pseudovirus infection in the presence of inhibitor for a period of 48 h. X-axis indicates log concentration of inhibitor. Y axis indicates percentage inhibition compared to pseudovirus infection without inhibitor treatment. Results shown are representative of three independent experiments.

    Journal: bioRxiv

    Article Title: Characterization of spike processing and entry mechanisms of seasonal human coronaviruses NL63, 229E and HKU1

    doi: 10.1101/2024.04.12.589332

    Figure Lengend Snippet: (A) Chloroquine sensitivity of HCoV-NL63 and SARS-CoV-2 pseudoviruses in 293T-ACE2 and 293T-ACE2-TMPRSS2 cells. (B) Camostat mesylate sensitivity of HCoV-NL63 and SARS-CoV-2 pseudoviruses in 293T-ACE2 and 293T-ACE2-TMPRSS2 cells. (C) Chloroquine sensitivity of HCoV-229E pseudoviruses in 293T-ACE2-APN cells. (D) Ammonium chloride sensitivity of HCoV-229E pseudoviruses in 293T-ACE2-APN cells. (E) Chloroquine sensitivity of HCoV-HKU1 and SARS-CoV-2 pseudoviruses in 293T-TMPRSS2 and 293T-ACE2 cells. (F) Camostat mesylate sensitivity of HCoV-HKU1 and SARS-CoV-2 pseudoviruses in 293T-TMPRSS2 and 293T-ACE2-TMPRSS2 cells respectively. For all experiments, cells were pretreated with indicated inhibitor for 2 h prior to pseudovirus infection in the presence of inhibitor for a period of 48 h. X-axis indicates log concentration of inhibitor. Y axis indicates percentage inhibition compared to pseudovirus infection without inhibitor treatment. Results shown are representative of three independent experiments.

    Article Snippet: Pseudoviruses bearing the desired spike glycoprotein of HCoV-NL63, -229E, -HKU1 and SARS-CoV-2 and carrying a firefly luciferase (FLuc) reporter gene were produced by cotransfecting HEK-293T (ATCC, Cat no: CRL-11268) cells with 5 μg of pCMVΔR8.2, 5 μg of pHR’CMVLuc and 0.5 μg of pVRC spike or its corresponding mutant expression plasmids.

    Techniques: Infection, Concentration Assay, Inhibition

    Analysis of interferon (‐response) genes upon coronavirus infection and effects of IFN‐λ1 supplementation on viral load. (a) Volcano plots depicting shared gene expression profiles of bronchial epithelial cell cultures infected with SARS‐CoV, SARS‐CoV‐2 or MERS‐CoV for (a) 24, (b) 48 hpi or (c) 72 hpi in comparison to HCoV‐229E and HCoV‐OC43; n = 4. Red dots indicate significantly upregulated genes, and blue dots indicate significantly downregulated genes. (d) Heatmap of the overlap of significantly changed genes during 24, 48 and 72 hpi infection with SARS‐CoV‐2, SARS‐CoV, MERS‐CoV or HCoV‐229E or HCoV‐OC43 and mock as uninfected control. (e) Gene set variation analysis (GSVA) was performed on interferon response genes after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. As HCoV‐OC43 infection was conducted at 33°C, SARS‐CoV‐2 infection at 33°C was included as control (pink). (f) IFN‐λ1 and (g) IFN‐α1 gene expression after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. The error bar indicates the standard deviation. Significant differences in increased gene expression over time of infection ( P < 0.05) are shown by the * symbol. Two‐way ANOVA followed by an unprotected Fisher's least significance difference test was conducted to test for significance. (h) ALI‐PBEC cultures were pretreated with 5 ng mL −1 interferon lambda (IFN‐λ1) for 60 min and subsequently infected with SARS‐CoV‐2 for 3 days with IFN‐λ1 present in the basal medium. Extracellular viral RNA copies are depicted in log scale. Data in h were analysed with a paired t ‐test and are depicted as mean ± SEM. n = 4 independent donors.

    Journal: Clinical & Translational Immunology

    Article Title: SARS ‐ C o V ‐2‐infected human airway epithelial cell cultures uniquely lack interferon and immediate early gene responses caused by other coronaviruses

    doi: 10.1002/cti2.1503

    Figure Lengend Snippet: Analysis of interferon (‐response) genes upon coronavirus infection and effects of IFN‐λ1 supplementation on viral load. (a) Volcano plots depicting shared gene expression profiles of bronchial epithelial cell cultures infected with SARS‐CoV, SARS‐CoV‐2 or MERS‐CoV for (a) 24, (b) 48 hpi or (c) 72 hpi in comparison to HCoV‐229E and HCoV‐OC43; n = 4. Red dots indicate significantly upregulated genes, and blue dots indicate significantly downregulated genes. (d) Heatmap of the overlap of significantly changed genes during 24, 48 and 72 hpi infection with SARS‐CoV‐2, SARS‐CoV, MERS‐CoV or HCoV‐229E or HCoV‐OC43 and mock as uninfected control. (e) Gene set variation analysis (GSVA) was performed on interferon response genes after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. As HCoV‐OC43 infection was conducted at 33°C, SARS‐CoV‐2 infection at 33°C was included as control (pink). (f) IFN‐λ1 and (g) IFN‐α1 gene expression after 24, 48 and 72 hpi ( n = 4) in highly pathogenic (orange) and low pathogenic (blue) coronavirus‐infected cultures. The error bar indicates the standard deviation. Significant differences in increased gene expression over time of infection ( P < 0.05) are shown by the * symbol. Two‐way ANOVA followed by an unprotected Fisher's least significance difference test was conducted to test for significance. (h) ALI‐PBEC cultures were pretreated with 5 ng mL −1 interferon lambda (IFN‐λ1) for 60 min and subsequently infected with SARS‐CoV‐2 for 3 days with IFN‐λ1 present in the basal medium. Extracellular viral RNA copies are depicted in log scale. Data in h were analysed with a paired t ‐test and are depicted as mean ± SEM. n = 4 independent donors.

    Article Snippet: Next‐generation sequencing was performed, and the isolate was mapped to the HCoV‐OC43 strain ATCC VR‐759 (GenBank: AY585228.1).

    Techniques: Infection, Expressing, Comparison, Standard Deviation